Pharmacy and Pharmaceutical Sciences Commons^™

Research News: 2014, University Of Mississippi. School Of Pharmacy

Research News: Grants and Publications (2000-2014)

Grants and contracts recently awarded

Efficacy Of Female Rat Models In Translational Cardiovascular Aging Research, Kevin M. Rice, J. C. Fannin, C. Gillette, Eric Blough

Pharmaceutical Science and Research

Cardiovascular disease is the leading cause of death in women in the United States. Aging is a primary risk factor for the development of cardiovascular disease as well as cardiovascular-related morbidity and mortality. Aging is a universal process that all humans undergo; however, research in aging is limited by cost and time constraints. Therefore, most research in aging has been done in primates and rodents; however it is unknown how well the effects of aging in rat models translate into humans. To compound the complication of aging gender has also been indicated as a risk factor for various cardiovascular diseases. …

Hemagglutinin-Based Polyanhydride Nanovaccines Against H5n1 Influenza Elicit Protective Virus Neutralizing Titers And Cell-Mediated Immunity., Kathleen A. Ross, Hyelee Loyd, Wuwei Wu, Lucas Huntimer, Shaheen Ahmed, Anthony R. Sambol, Scott Broderick, Zachary Flickinger, Krishna Rajan, Tatiana K. Bronich, Surya Mallapragada, Michael J. Wannemuehler, Susan Carpenter, Balaji Narasimhan

Journal Articles: Pharmaceutical Sciences

H5N1 avian influenza is a significant global concern with the potential to become the next pandemic threat. Recombinant subunit vaccines are an attractive alternative for pandemic vaccines compared to traditional vaccine technologies. In particular, polyanhydride nanoparticles encapsulating subunit proteins have been shown to enhance humoral and cell-mediated immunity and provide protection upon lethal challenge. In this work, a recombinant H5 hemagglutinin trimer (H5₃) was produced and encapsulated into polyanhydride nanoparticles. The studies performed indicated that the recombinant H5₃ antigen was a robust immunogen. Immunizing mice with H5₃ encapsulated into polyanhydride nanoparticles induced high neutralizing antibody titers and enhanced CD4(+) T …

Efek Fraksi N-Heksana Kulit Hylocereus Polyhizus Terhadap Kadar Malondialdehida Tikus Stres Oksidatif, Wirna Maya Sari, Sri Wahdaningsih, Eka Kartika Untari

Pharmaceutical Sciences and Research

Excessive free radical production will lead to oxidative stress. One of the indicators used to determine stress oxidative is malondialdehyde (MDA). Hylocereus polyhizus peel is potential used natural exogenous antioxidant. The aim of this research was to study the effect of n-hexane fraction of H.polyhizus peel on MDA level and to determine the dose that could reduce the MDA level. H.polyhizus peel was macerated with chloroform, fractionated with n-Hexane. Rats were divided into 7 groups : normal, negative, positive control vitamin E (18 mg/ kgbw), positive control quercetin (4 mg/200gbw), H.polyhizus peel extract group treated with dose I (10mg/ 200gbw), …

Antibakteri Fraksi N-Heksana Kulit Hylocereus Polyrhizus Terhadap Staphylococcus Epidermidis Dan Propionibacterium Acnes, Sri Wahdaningsih, Eka Kartika Untari, Yunita Fauziah

Pharmaceutical Sciences and Research

Acne can be caused by Staphylococcus epidermidis and Propionibacterium acnes. By using antibiotic for the acne theraphy in a long term period can cause resistance, organ detriment and imunohypersensitivity. Red dragon fruit’s peel (Hylocereus polyrhizus) is one of natural materials which can be used for alternative antibacterial. This study aims to determine the antibacterial activity of n-Hexane fraction of red dragon fruit’s peel against S. epidermidis dan P. acnes by the method of disc diffusion Kirby-Bauer. Red dragon fruit’s peel powder macerated within chloroform, and macerate then fractionated with n-Hexane. Phytochemical screening result red dragon fruit’s peel contains terpenoid and …

Efek Fraksi N-Heksana Kulit Hylocereus Polyrhizus Terhadapaktivitas Katalase Tikus Stres Oksidatif, Eka Kartika Untari, Sri Wahdaningsih, Agustia Damayanti

Pharmaceutical Sciences and Research

Production of excess free radicals cause oxidative damage and degenerative diseases. Catalase enzyme is one of the endogenous intracellular antioxidant that can be reduced by oxidative stress conditions. The peel of red dragon fruit (Hylocereus polyrhizus) as a potent exogenous antioxidants could support the internal enzyme activity to prevent free radicals. The aim of this research was to determine the effect of n-hexane fraction of H.polyrhizus’s peel and the dose that could increase activity of catalase. The peel was maserated with chloroform, then fractionated by n-hexane. Stress condition was achieved by five days fasting and swimming for 10 mins/day to …

Formulasi Krim Antioksidan Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas (L.) Lamk) Sebagai Anti Aging, Damaranie Dipahayu, Widji Soeratri, Mangestuti Agil

Pharmaceutical Sciences and Research

No abstract provided.

Uji Stabilitas Fisik Formula Krim Yang Mengandung Ekstrak Kacang Kedelai (Glycine Max), Rosmala Dewi, Effionora Anwar, Yunita Ks

Pharmaceutical Sciences and Research

Soybean extract has known for its antiaging potential. The addition of soybean extract was predicted can influence the physical stability of the cream. Therefore, this research was aimed to test the physical stability of cream contained soybean extract in different concentration (2%, 4%, 6%, and 8%). The physical stability test including the storage for eight weeks at room temperature, high temperature (40°±2°C), and low temperature (4°±2°C). Stability parameters were the organoleptic observation, pH, globul size, viscosity, and cycling test. Those four cream formula showed good stability in organoleptic, pH, globul size, viscosity, and cycling test.

Design And Development Of A Novel Class Of Cell Cycle Cdk Inhibitors Targeting The Cyclin Binding Groove Utilizing The Replace Strategy, Padmavathy Nandha Premnath

Theses and Dissertations

Inhibition of CDK2 activity in G1 and S phases of the cell cycle can promote selective apoptosis of cancer cells through the E2F1 pathway. Currently available CDK inhibitors target the ATP binding pocket and result in lack of specificity for the cell cycle vs. the transcriptional CDKs. It has been shown that a peptide HAKRRLIF derived from the tumor suppressor p21 binds to the cyclin binding groove (CBG) and selectively inhibits cell cycle CDKs (CDK2/Cyclin A, CDK2/Cyclin E and CDK4/Cyclin D). The CBG is unique to cell cycle CDKs hence targeting this site avoids the inhibition of transcriptional CDKs can …

Effect Of Formulation Variables On Preparation Of Celecoxib Loaded Polylactide-Co-Glycolide Nanoparticles, Dustin L. Cooper, Sam Harirforoosh

Pharmacy Faculty Articles and Research

Polymer based nanoparticle formulations have been shown to increase drug bioavailability and/or reduce drug adverse effects. Nonsteroidal anti-inflammatory drugs (e.g. celecoxib) reduce prostaglandin synthesis and cause side effects such as gastrointestinal and renal complications. The aim of this study was to formulate celecoxib entrapped poly lactide-co-glycolide based nanoparticles through a solvent evaporation process using didodecyldimethylammonium bromide or poly vinyl alcohol as stabilizer. Nanoparticles were characterized for zeta potential, particle size, entrapment efficiency, and morphology. Effects of stabilizer concentration (0.1, 0.25, 0.5, and 1% w/v), drug amount (5, 10, 15, and 20 mg), and emulsifier (lecithin) on nanoparticle characterization were examined …

Pharmacist Consult Service In Geriatric Trauma Patients, Vanessa M. Gleason, Pharm D., Rohit A. Moghe, Pharm D., Msph, Cde, Doris Warner, Msn, Rn, Cen, Michael S. Weinstein, Md, Facs

Pharmacy Presentations, Posters, and Grand Rounds

Introduction

• Geriatric patients (> 65 years of age) have a 3-5% incremental increase in annual occurrence of trauma making it a top five leading cause of death in this population.

• The mortality rate ranges from 15-30%, which is 4-5 times higher than younger patients.

• In 2011, data from the Pennsylvania Trauma Systems Foundation (PTSF) demonstrated nearly 32% of injured patients statewide and nearly 50% of the trauma patients at Thomas Jefferson University Hospital (TJUH) were > 65 years.

• Most geriatric patients have comorbidities that are treated with many medications, which may predispose them for falls.

• Although …

Pharmacy Student-Led Evaluation Of Patient Willingness For Smoking Cessation Utilizing Various Smoking Cessation Methods, Anita Peña, Matthew Stryker, Angela Nagel, Nabila Ahmed-Sarwar

Pharmacy Faculty/Staff Publications

Purpose: The ever-growing variety of methods available for smoking cessation have the potential to lead to confusion influencing a patient’s willingness to quit smoking. The aim of this study is to investigate if a change in patient motivation and product preference has occurred after receiving education on the various smoking cessation products

Methods : An informative poster was created to highlight different smoking cessation methods .A questionnaire was developed to assess patient motivation to quit utilizing the ‘Readiness to Quit Ladder’ and patient experiences with or preference between various smoking cessation methods.1The poster was displayed in various pharmacy settings, including …

Retrospective Study To Identify Predictors Of Withdrawal In Pediatric Intensive Care Patients On Long-Term Sedation, Lindsay Fakete Pharmd, Jenny Boucher Pharmd, Jd, Bcps, Scott Wheatley Md

Department of Pharmacy

No abstract provided.

Exploring The Attitudes, Knowledge, And Receptivity Of Community-Working Pharmacists Regarding Two Over-The-Counter Medications (Otcs), Robin Spielmann, John Redwanski

Pharmacy Practice Faculty Posters

Many patients go without access to contemporary or traditional Western medicine due to lack of quality health insurance. Thus, more patients are turning to other less expensive, alternative forms of treatment. Our analysis of several different community-pharmacies in Maine, Indiana, Ohio, and Kentucky showed that many practicing pharmacists know little about these types of alternative therapies, and make little effort answering questions and/or making a safe recommendation about these products. Two OTCs were chosen with purpose: Calcium with Vitamin D and Grape Seed Extract. A convenience sample of pharmacies and pharmacists were selected based on travel time and location. Pharmacists …

Study Of Novel Nanoparticle Transport And Drug Release For Cancer Treatment., Christopher Gene England 1988-

Electronic Theses and Dissertations

Nano-scale particles sized 10—400 nm administered systemically preferentially extravasate from tumor vasculature due to the enhanced permeability and retention effect. Therapeutic success remains elusive, however, because of inhomogeneous particle distribution within tumor tissue. Insufficient tumor vascularization limits particle transport and also results in avascular hypoxic regions with non-proliferating cells, which can regenerate tissue after nanoparticle-delivered cytotoxicity or thermal ablation. In this study, gold nanoparticles were functionalized with phosphatidylcholine (two-layer) or phosphatidylcholine and HDL (three-layer) in the formation of “layered” nanoparticles. The diffusivity of both two- and three layered colloidal gold nanoparticles and silica gold nanoshells were examined in 3D cell …

Extracellular Matrix Proteins And The Liver-Lung Axis In Disease., Veronica L. Massey

Electronic Theses and Dissertations

No abstract provided.

Top 10 Medicinal Plants And Their Groundbreaking Impact On Modern Medicine, Jordan N. Patterson, Joan Marie Lausier

Senior Honors Projects

This paper describes ten plants that having been groundbreaking drugs in the past century that were all derived from plants. I chose to do my project on this topic because of my family’s history with natural medicine. In 1999 my mother was diagnosed with Bile Duct cancer and the doctors gave her only a couple of weeks to live. The doctors recommended chemotherapy and radiation, but the results seemed negative for the situation. After some extensive research, my parents found St. Georges Medicinal Wellness Center located in the tiny village of Bad Aibling, Germany. This hospital along with offering traditional …

The Differential Effects Of Fructose And Glucose On Advanced Glycation End-Product Formation And Cellular Damage In Vitro, Regina Blackley, Matthew Stryker, Susan Denapoli, Melinda E. Lull

Doctoral External Publications

The protective mechanisms of microglia cells help to maintain central nervous system (CNS) homeostasis and function.1 Microglia are innate immune cells that constantly survey their surrounding CNS microenvironment for pathogens, damaged cells, and inflammatory molecules. Sugars from our diet, including glucose and fructose, combine with endogenous proteins non-enzymatically and form advanced glycation-end products (AGEs).2 AGEs are shown to produce reactive oxygen species, leading to inflammation and cellular damage that may be mediated by microglia.3 Fructose consumption has become increasingly prevalent within the American diet, as it is a lower cost sweetener.4 Microglia become activated and phagocytic in the presence of …

Altered Cell-Surface Receptor Levels Result From Fructose Advanced Glycation End Product-Induced Inflammation, Matthew Stryker, Regina Blackley, Melinda E. Lull

Doctoral External Publications

Objective: As a result of the heightened reactivity fructose demonstrates compared to glucose and our current knowledge of glucose advanced glycation end-products, the aim of this research was to further elucidate the proinflammatory pathways involved in the response to fru-AGE exposure, including the effects of fru-AGEs on cell-surface receptor expression. We hypothesized that once microglia were activated in response to fru-AGE exposure, there would be an increase in the expression of RAGE and TLR4 to facilitate the proinflammatory cascade.

Educating Pharmacists And The Public About The Role Of Over-The-Counter Medications In The Management Of Autism Spectrum Disorders, Jillian M. Bankoff, Melinda E. Lull

Doctoral External Publications

Over-the-counter (OTC) medications are used by various populations as an adjunct to help improve various aspects of life, such as sleep cycles, disease prevention, and mood. The purpose of this study is to compile all of the available data from human trials of over-the-counter medications used in patients with Autism Spectrum Disorders (ASD). OTCs have been used in the treatment of ASD to minimize social impairments, suppress repetitive behaviors, and enhance quality of sleep to improve daytime behaviors. Ultimately, the main objectives of this project are to educate pharmacists and the public (both patients and their families) about the role …

Development And Implementation Of Peer-Led Weekly Review Sessions And The Assessment Of Their Impact On Student Learning, Alyssa L. Ashworth, Inlok Lam, Nicole E. Moore, Melinda E. Lull

Doctoral External Publications

Peer tutoring services for a pathophysiology and therapeutics course have historically been made available to pharmacy students, but they were greatly underutilized. The role of peer tutors had been exclusively to hold office hours during which students could ask questions or review material. Peer tutoring services were updated and expanded based on the needs of the students. The objectives of this project were to develop, implement, and assess the impact of peer-led organized weekly review sessions in a pathophysiology and therapeutics course.

An Examination Of The Effects Of Atorvastatin And Parathyroid Hormone On Osteoblast Activity, Inlok Lam, Andrea Traina, Melinda E. Lull

Doctoral External Publications

HMG-CoA reductase inhibitors, also known as statins, are a ubiquitous class of medication used for lowering cholesterol. In-vitro and animal studies have suggested that statins can activate osteoblast differentiation and have anabolic effects on bones; however, observational and experimental studies in humans have shown conflicting results.1-5 The exact mechanism of statins on bone growth is unknown; however, there are several hypotheses. The “Lipid Hypothesis” (Figure 1) suggests that lipid oxidation leads to activation of PPARγ, and production of isoprostanes including isoPGF2α and isoPGEα. PPARγ is associated with inhibition of osteoblast differentiation, while isoprostanes markers are associated with the induction of …

Formulation And Stability Of An Extemporaneously Compounded Oral Solution Of Chlorpromazine Hcl, Daniel L. Prohotsky, Katherine Juba, Fang Zhao

Pharmacy Faculty/Staff Publications

Chlorpromazine is a phenothiazine antipsychotic which is often used in hospice and palliative care to treat hiccups, delirium, and nausea. With the discontinuation of the commercial oral solution concentrate, there is a need to prepare this product by extemporaneous compounding. This study was initiated to identify an easy-to-prepare formulation for the compounding pharmacist. A stability study was also conducted to select the proper storage conditions and establish the beyond-use date. Chlorpromazine HCl powder and the Ora-Sweet® syrup vehicle were used to prepare the 100 mg/mL solution. Once the feasibility was established, a batch of the solution was prepared and packaged …

Lunasin Reduces The Melanoma Stem Cell Population In Vitro And Inhibits Tumor Proliferation In Vivo., Christopher Paul Shidal

Electronic Theses and Dissertations

Lunasin is a 44 amino acid peptide derived from the soybean seed that has been shown to have cancer chemopreventive and chemotherapeutic properties. In this study, we investigated the potential utility of lunasin as a chemotherapeutic in a melanoma model. Initial studies showed that lunasin has little activity against established melanoma cell lines in vitro using adherent culture methods; however, lunasin’s in vitro activity was significantly higher in non-adherent colony-forming assays in soft agar and oncosphere assays. These results led us to investigate whether lunasin selectively affects cancer initiating cells (CIC) that are known to be present in these melanoma …

Impact Of An Interprofessional Communication Course On Nursing, Medical, And Pharmacy Students’ Communication Skill Self-Efficacy Beliefs, Nicholas E. Hagemeier, Rick Hess, Kyle S. Hagen, Emily L. Sorah

ETSU Faculty Works

Objective. To describe an interprofessional communication course in an academic health sciences center and to evaluate and compare interpersonal and interprofessional communication self-efficacy beliefs of medical, nursing, and pharmacy students before and after course participation, using Bandura’s self-efficacy theory as a guiding framework.

Design. First-year nursing (n=36), first-year medical (n=73), and second-year pharmacy students (n=83) enrolled in an interprofessional communication skills development course voluntarily completed a 33-item survey instrument based on Interprofessional Education Collaborative (IPEC) core competencies prior to and upon completion of the course during the fall semester of 2012.

Assessment. Nursing students entered the course with higher interpersonal …

Computer-Aided Drug Design And Discovery, Screening And Synthesis Of Small Molecule Inhibitors Of Nucleoside Transporters, Hilaire Colleen Playa

Theses and Dissertations (ETD)

Using prior biological data, pharmacophore models were made for hCNT1, hCNT3, hENT1, and hENT4. The hCNT3 and hCNT1 pharmacophore were used to select compounds for biological testing. The NBMPR analogue and dipyridamole analogue hENT1 pharmacophores were compared to each other and to a combined pharmacophore for hENT1. The dipyridamole analogue pharmacophore better predicted non-nucleoside small molecule inhibitors, and as such appears to be the better tool for aiding in the design of new small molecule inhibitors. The hCNT3 pharmacophore failed to select active compounds and as such must be redesigned. The hCNT1 pharmacophore succeeded in identifying two moderately active compounds …

Targeted Delivery Of Anti-Inflammatory And Anti-Oxidant Drugs For Treatment Of Chronic Obstructive Pulmonary Diseases, Yun Xiang

All Dissertations

Chronic obstructive pulmonary disease (COPD) was found to be the fourth leading cause of death in 2004, but current treatments can only relieve the symptoms and improve the quality of life for the patients. Glucocorticoids have been broadly applied in other inflammatory conditions with promising therapeutic effects, but performed poorly in COPD. The phenomenon called glucocorticoid resistance is responsible for glucocorticoid insensitivity in COPD. Researchers have proven that elevated oxidative stress in the lung of a COPD patient is one of the main causes of blocking the function of glucocorticoids. We hypothesize that reducing the oxidative stress in the lung …

Redesign Of Trans-Splicing Molecules For The Correction Of Dystrophia Myotonica Type 1 Toxic Rna Transcripts, Eleanor G. Harrison

Undergraduate Honors Theses

Dystrophia myotonica (DM1), one of the most common forms of muscular dystrophy, is caused by a repeated trinucleotide expansion in the DMPK gene. This mutation results in the accumulation of toxic cellular RNA transcripts. Spliceosome-mediated RNA trans-splicing (SMaRT) technology is a form of gene therapy that possesses the potential to correct these toxic RNA transcripts and thus cure the disease. Despite its promise, prior research applications of SMaRT technology to DM1 have been hampered by poor efficiency and have not been validated in a relevant model of the disease. In order to improve the efficiency of trans-splicing, this study examined …

Pharmacy Newsletter : December 2014, Pharmacy Department

Department of Pharmacy Archives

Content:

• New WHO Guide to Prevent and Control Cervical Cancer
• Analgesic use in Adult Patients with Advanced Chronic Liver Disease or Cirrhosis
• The Novel Treatment of Clostridium Diffi cile Associated Diarrhea (CDAD)
• 25th September, World Pharmacist Day-Celebration

The Role Of Claudins In Bone Metabolism, Fatima Zakariya Alshbool

Loma Linda University Electronic Theses, Dissertations & Projects

Osteoporosis, a major public health problem in the U.S., is characterized by low bone mass and structural deterioration of bone tissue. Since the imbalance between bone formation and bone resorption during bone remodeling has been documented to be a major factor in the pathogenesis of osteoporosis, it is crucial to identify novel genes and/or novel functions of known genes that regulate the formation and activity of bone forming osteoblasts and bone resorbing osteoclasts. To this end, recent evidence suggests a significant role for caudins (Cldns), which comprise a major group of tight junction proteins, in bone biology and development. This …