Pharmacy and Pharmaceutical Sciences Commons^™

The Efficacy Of Cannabis As A Substitute Addiction For Opiates, Clark Furlong

Theses and Graduate Projects

Background: Approaches to opiate addiction focus on abstinence and/or a harm reduction model that often utilizes substitute medications. Methadone and suboxone are the most prescribed drugs for opiate maintenance therapy. The long-term side effect profile and health consequences are undesirable. Research is looking for healthier alternatives.

Purpose: Opiate maintenance therapy participants have a mortality rate greater than twelve times that of the normal population, we seek to discover if cannabis can be a useful/safer alternative.

Methods: Following a comprehensive literature review a 7-question online survey was designed with SurveyMonkey to gauge current Augsburg students’ perception of cannabis as an alternative …

Enhancing The Anticancer Effects Of Apiaceae Spice Phytochemicals., Jared Lee Scott

Electronic Theses and Dissertations

Breast cancer (BC) is a leading contributor to the death of women and is the most common type of cancer. Many chemotherapy drugs are developed from plants, and some plant extracts can exhibit significant anticancer activities while also having less toxic side effects. The Apiaceae plant family consists of several species that are used as culinarily spices which have demonstrated antioxidant, chemopreventive, and anticancer activities. The encapsulation of anticancer phytochemicals into naturally produced membrane-bound nanoparticles known as exosomes could improve their systemic distribution and efficacy. Exosomes are involved in cell-to-cell communication through the delivery of proteins, nucleic acids, and small …

Microencapsulation Of Cosmos Caudatus Kunth Extract Using Sodium Alginate And In Vitro Antioxidant Activity Test, Safina Samara Nur Fadlila, Oktavia Eka Puspita, Zubaidah Ningsih, Anna Safitri

Makara Journal of Science

This study aims to encapsulate Cosmos caudatus K. leaf extract in sodium alginate crosslinked with calcium chloride (CaCl₂) as coating materials through freeze-drying. Antioxidant assays were performed to evaluate the applicability of the microcapsules as antidiabetic medicines. Their characteristics, antioxidant activity, and encapsulation efficiency (EE) were also examined. Results indicated that the concentration of sodium alginate and pH influenced the manufacturing process of the microcapsules. The highest EE was obtained at pH 6 and alginate concentration of 2% (w/v). The IC₅₀ for the antioxidant activity of the microcapsules was 139.96 ± 1.094 μg/mL. Scanning electron microscopy confirmed …

Molecular Docking Of The Interaction Between Citrus Amblycarpa Extract Contents And Inflammatory Proteins Of Hepatic Steatosis, Lena Rosida, Dewi Indah Noviana Pratiwi, Roselina Panghiyangani, Juliyatin Putri Utami, Nasrul Idhafi, Muhammad Febriansyah, Andi Azizah Maulidia Budiarman

Makara Journal of Science

Citrus amblycarpa possesses various pharmacological activities, such as antioxidant, anticancer, antitumor, hepatoprotective, anti-inflammatory, antidiabetic, antiviral, antibacterial, and antifungal. The main active compounds in C. amblycarpa (including gamma (γ)-aminobutyric acid [GABA], hesperidin, naringin, neoeriocitrin, poncirin, quercetin, and rutin) show potential to interact with the inflammatory proteins in hepatic steatosis (such as nuclear factor kappa beta [NF-kB], tumor necrosis alpha [TNF-alpha], interleukin-6 [IL-6], c-Jun NH2-terminal kinase [JNK], and adiponectin). Molecular docking simulations were performed using Swiss Dock (http://www.swissdock.ch/), and analysis and visualization were conducted using Discovery Studio 4.1. Rutin, poncirin, hesperidin, and neoeriocitrin exhibit high affinities to NF-κB, TNF-alpha, IL-6, and adiponectin …

Efficacy Of Ferula L. Species Extracts From Tajikistan Against Influenza Viruses, Saidbeg Satorov, Sulhiy Mavlonazarova, Ahdrey P. Bogoyavlenskiy, Salomuddin D. Yusufi, Vyacheslav Dushenkov

Publications and Research

Efficacy of Ferula L. Species Extracts from Tajikistan Against Influenza Viruses One of the challenges facing both the domestic and international pharmaceutical industries is the limited selection and suboptimal efficacy of available antiviral drugs. The high mutational variability of influenza viruses contributes to rapid acquisition of drug resistance, which diminishes or nullifies the effectiveness of existing therapies, thereby necessitating the exploration for novel therapeutics. This study aimed to assess the antiviral activities of three species of the genus Ferula— indigenous to the Republic of Tajikistan—against influenza strains A/Vladivostok/2/09 (H1N1) and A/Almaty/8/98 (H3N2). The investigation revealed that phytochemical constituents—specifically, root …

Comparative Analysis Of Polyphenol Content, Antioxidant Activity And Antimicrobial Properties In Endemic And Widespread Allium Species Of Tajikistan, Fazila Mirzoeva, Saidbeg Satorov, Salomuddin Usufi, Vyacheslav Dushenkov, Shukhratdzhon Shukhratdzhon

Publications and Research

In Tajikistan, the Allium genus is notably prolific, with over 80 species documented, and new species continually being identified in the mountainous regions. The Gorno- Badakhshan Autonomous Region (GBAO) of Tajikistan, within the Pamir Mountains, is significant for its 31 distinct Allium species, including endemics like A. afghanicum Wendelbo and A. schugnanicum Vved. The distribution of these species is often geographically restricted, and their biological activities, especially their antimicrobial properties, remain largely unexplored, presenting significant opportunities for future phytochemical and pharmacological research. This scholarly inquiry is aimed at contributing substantially to the academic discourse on the therapeutic capacities of Allium …

Phytochemical Assay And In – Vitro Cytotoxicity Assessment Of Cassava Peel (Manihot Esculenta) From Ethanol And Ethyl Acetate Extract Against Cervical Cancer Cells (Hela), Alice Hari Soejono, Surya Dwira, Puji Sari

Indonesian Journal of Medical Chemistry and Bioinformatics

Introduction: Cervical cancer is a type of cancer that develops in a woman’s cervix. It is a result of HPV (Human Papilloma Virus) and is one of the deadliest types of cancer. Some cervical cancer management including chemotherapy, which until recently continues to develop to cure the condition, is relatively expensive and comes with multiple different side effects.

Method: Manihot esculenta undergoes multilevel maceration with the solvent of ethanol and ethyl acetate. This leads to the formation of ethanol extract and ethyl acetate extract of Manihot esculenta that is analyzed through phytochemical assay and thin layer chromatography (TLC) to determine …

Proteomic Characterization And Cytotoxic Potential Of Proteins From Cuscuta (Cuscuta Epithymum (L.) Crude Herbal Product Against Mcf-7 Human Breast Cancer Cell Line, Umaima Akhtar, Yamna Khurshid, Bishoy El-Aarag, Basir Syed, Ishtiaq A. Khan, Keykavous Parang, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

The burden of breast cancer, the second leading cause of death worldwide, is increasing at an alarming rate. Cuscuta, used in traditional medicine for different ailments, including cancer, is known for containing phytochemicals that exhibit anticancer activity; however, the bioactivities of proteins from this plant remain unexplored. This study aimed to screen the cytotoxic potential of proteins from the crude herbal product of Cuscuta epithymum(L.) (CE) harvested from the host plants Alhagi maurorum and Medicago sativa.

Methods

The proteins from CE were extracted using a salting-out method, followed by fractionation with a gel filtration chromatography column. Gel-free shotgun …

Investigating Effects Of Co-Culturing With Vibrio Fischeri On Secondary Metabolite Production Of Hawaiian Bobtail Squid Symbionts, Mariam A. Zedan

Honors Scholar Theses

Microbes are a fruitful source of natural product drug discovery since many of their secondary metabolites show defensive activity and have unique structures. However, extracting and elucidating the structure of those compounds can be challenging in vitro due to particular environmental and nutritional requirements of some bacteria. Those requirements may include proximity to other organisms that the microbes have symbiotic relationships with. Under those conditions, the organism may activate silent biosynthetic gene clusters (BGCs) responsible for production of certain secondary metabolites not typically expressed otherwise. One method of BGC induction is co-culturing with other microbes since this mimics the natural …

Antioxidant Activities And Protective Effects Of Shorea Macrophylla Leaf And Bark Extracts, Salfarina Ramli, Nik Nor Sabrina Sukri, Nur Adhwa’ Faqihah Zulkifli, Donna Maretta Ariestanti, Chee Yan Choo, Hasseri Halim, Nurhuda Manshoor, Richard Johari James

Pharmaceutical Sciences and Research

Shorea macrophylla, also known as the ‘Engkabang’ tree or Light Red Meranti, is renowned for its role in reforestation efforts. Despite lacking records of traditional use, the reported biological activities of other species within the Shorea genus spark curiosity about the potential biological activities of this plant. Therefore, this study aims to explore the antioxidant capabilities of S. macrophylla leaf and bark extracts, along with their protective effects against oxidative stress using a brine shrimp model. In the evaluation of antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays were employed. Extracts were incubated in …

A New Approach To Balance Dental Fear And Anxiety By Using Bachtm Flower Therapy, Irina Maria Gheorghiu, Alexandru Andrei Iliescu, Stana Paunica, Marina Giurgiu, Brandusa Mocanu, George Alexandru Popescu, Anca Silvia Dumitriu

Journal of Mind and Medical Sciences

Treatments in dentistry currently consist of an interdisciplinary approach, including (but not necessarily limited to) the holistic perspective. The different fields of allopathic and complementary medicine are used together to ensure not only a high-quality restorative treatment, but also to provide patients with psychological and emotional support. This perspective also applies to dental anxiety, which consists of complex (emotional, vegetative and psychomotor) manifestations. One of the most well-known complementary therapies for reducing dental fear and anxiety is BachTM Flower Therapy. Even if the mechanism of action of this therapy is not yet scientifically documented, notable results have been and continue …

Antioxidant, Tyrosinase Inhibition Activity, And In Vitro Spf Evaluation Of Pepino Fruit Extract (Solanum Muricatum Aiton) In Different Solvent Types And Concentrations, Nining Sugihartini, Girly Risma Firsty, Wahyu Kumil Laila, Sri Mulyaningsih, Ichwan Ridwan Rais

Pharmaceutical Sciences and Research

Solvent is a key factor that affects the effectiveness of active compound extraction from plant materials (simplisia). This study aimed to determine the optimal type and concentration of solvent used in the extraction of pepino fruit based on the parameter of antioxidant and tyrosinase inhibition activity, as well as Sun Protection Factor (SPF) value. The extraction was carried out using the maceration method with ethanol or ethyl acetate as the solvent, each at concentrations of 50%, 70%, and 96%, respectively. The antioxidant activity of the extracts was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The inhibition of tyrosinase and …

Beyond Mitragynine: Composition Survey And Stability Assessment Of Kratom Tea Ordered Via Food Delivery Platforms In Bangkok, Thailand, Yuta Tokuda, Apinya Tubtimrattana, Nat Tansrisawad, Kornvalee Meesilpavikkai, Parath Thirati

Chulalongkorn Medical Journal

Background: Kratom is a native tree to Southeast Asia. Kratom leaves have long been used medicinally and recreationally due to their stimulative and opioid-like effects attributed to high endogenous levels of mitragynine and related alkaloids. Kratom is widely consumed as tea and is now publicly sold, including on food delivery platforms, after recent decriminalization despite selling of kratom products being still considered illegal by other acts.

Objectives: To assess the formulas of kratom teas sold on Thai food delivery platform together with their consistency and the stability of refrigerated teas.

Methods: Kratom alkaloids, mitragynine and 7-hydroxymitragynine, and additive contents of …

Chemical And Biological Investigation Of The Effects Of Vinegar Baking Sambucus Canadensis Anthocyanins, Elizabeth Martin

ATU Scholars Symposium

New treatments for cancer and bacterial infections are needed, especially because most patients acquire resistance to conventional first-line treatments. Many natural product metabolites exhibit potent activity. Sambucus sp., elderberry, and Salvia sp., or sage, are well-known shrubs for their therapeutic benefits. This study aims to analyze how secondary metabolites are extracted from the native American Elderberry, Sambucus canadensis, and Sage, Salvia officinalis. It also aims to investigate the process of vinegar baking of medicinal plants to enhance the chemical space and increase the biological properties. Vinegar baked and non-vinegar baked elderberries and sage were utilized to test this theory. …

The Quantification Of Essential Oils And Bixin In Annatoo Seeds, Alycia C. Johnson, Susan Oxley

Posters - 2024

Bixa orellana (also known as annatto or achiote) originates from South America but it can be found nearly everywhere in the world. The seeds of this species make for an excellent natural coloring. It is applied to makeup, food, and textiles; some of the biggest commercial industries.1 Of the 50+ phytochemicals identified, there are a large number of essential oils and other compounds that have been shown to have beneficial properties. Four of these compounds will be used to determine which traditional processing method results in the biggest loss of phytochemicals. Annatto is often either fried, left whole, or powdered …

Advancements In The Synthesis Of Polyoxygenated Oxepanes And Thiepanes For Applications To Natural Products, Aditya R. Pote, Shayne M. Weierbach, Mark W. Peczuh, Kyle M. Lambert

Chemistry & Biochemistry Faculty Publications

Oxepanes are central motifs and tenants of many biologically important molecules, and their synthetic construction often presents a challenge to chemists due to consequential entropic and enthalpic barriers that have limited the synthetic toolbox to access these seven-membered oxacycles. This review covers the breadth of synthetic methods to afford the oxepane/thiepane moiety, with a focus on polyoxygenated oxepanes and includes radical cyclizations, Lewis acid-mediated cyclizations, ring closing-metathesis, Nicholas-Ferrier rearrangement, homologations, and ring-expansion strategies. Implementation of these tactics towards sugar-based and non-sugar based (de novo) approaches is presented alongside their extensive application to the total synthesis of several complex polyoxygenated oxepane-containing …

Tracing Antibacterial Compounds From Kaledang (Artocarpus Lanceifolius Roxb.) Stem Bark, Hamsidar Hasan, Tety Tuloli, Syamsul Bahri, La Ode Aman

Pharmaceutical Sciences and Research

Artocarpus lanceifolius Roxb. or kaledang is a species of the mulberry family, Moraceae. Several extracts from its family have been reported to have antibacterial activity. Thus, it is necessary to determine compounds that have nature as antibacterial to be raw materials for antibacterial herbal medicines. The purpose of the study was to trace compounds that have antibacterial activity from Artocarpus lanceifolius stem bark. The study method was extraction by graded maceration, fractionation with column vacuum chromatography (CVC), isolation by radial chromatography, determination of antibacterial activity by agar diffusion method using the Gram positive and Gram negative bacteria. The characterization …

Antimicrobial Activity Of Crude Fractions Of Gossypium Barbadense Leaves And Isolation Of Stigmasterol, Maryam Saddiq, Ahmed Salisu, Kamaluddeen Kabir, Yusuf Hassan

Pharmaceutical Sciences and Research

Plants have been used in traditional medicine in different parts of the world to treat several diseases. Many studies have confirmed their medicinal values, which have led to the isolation and characterization of various potential drug candidates. This study focuses on the antimicrobial activity of crude fractions of Gossypium barbadense leaves and the isolation of active compound. The pulverized plant leave was soaked in ethanol (98% v/v), and a solvent-based fractionation was carried out using n-hexane, chloroform, ethyl acetate, and ethanol. The obtained crude fractions were screened for antimicrobial activity against four (4) clinical isolates: Escherichia coli, Pseudomonas …

Subchronic Toxicity Study Of Sterculia Rubiginosa Zoll. Ex Miq. Leaves Extract, Rini Prastiwi, Ester Hidayati, Riska Anggraini, Cut Mauliza, Ita Anggraini, Ema Dewanti

Pharmaceutical Sciences and Research

Ki hampelas (Sterculia rubiginosa Zoll. Ex Miq.) is traditionally used as an antiasthma. It also reported has antioxidant and nephroprotective activity. This study was conducted to evaluate the subchronic toxicity of the leaf extract of ki hampelas. The extract was orally administered to male and female Sprague-Dawley rats at doses of 50, 200, and 400 mg/kg bodyweight (BW) per day for 28 days. The rats were divided into four groups, consist of consist of normal group (Na CMC 0.5%), dose 1 (50 mg/kg BW), dose 2 (200 mg/kg BW), and dose 3 (400 mg/ kg BW) of extract. The …

Molecular Docking Analysis Of Acanthus Ilicifolius Compounds Toward Cul4b-Ddb1-Ahr-Erα Complex Protein For Antiosteoporosis Discovery, Binar Asrining Dhiani, Sarmoko Sarmoko, Retno Wahyuningrum, Akbar Yulianto

Pharmaceutical Sciences and Research

Osteoporosis represents a significant global public health issue, particularly among the aging population. Its incidence reaches 18.3% of the total population, with the highest prevalence observed in elderly postmenopausal women. A key factor in osteoporosis is the decreased expression level of estrogen receptor alpha (ERα), attributed to its degradation by the ubiquitin ligase protein complex Cullin4B (CUL4B), DNA damage binding 1 (DDB1), and aryl hydrocarbon receptor (AhR), collectively known as CUL4BAhR. Acanthus ilicifolius L contains compounds exhibiting antiosteoporosis activity, primarily by inhibiting osteoclastogenesis via RANKL. However, no reports exist of antiosteoporosis agents that act by inhibiting ERα degradation via CUL4BAhR. …

Exploring Rosella (Hibiscus Sabdariffa L.) Calyx Extracts As Natural Dye And Antioxidants In Lip Cream Product: Formulation And Evaluation, Donna Maretta Ariestanti, Abdul Mun'im, Joshita Djajadisastra, Riska Amalia Putri Hutami, Redhalfi Fadhila, Richard Johari James

Pharmaceutical Sciences and Research

Rosella, also known as Hibiscus sabdariffa L., is a plant that contains anthocyanin compounds in its calyx, which are known for their antioxidant properties. These compounds give the plant a captivating red color and can be used as natural dyes. This study aimed to exploit anthocyanins in rosella calyx as natural dyes and antioxidants in lip cream product. The formulations employed extracts from rosella calyx, a viscous extract from maceration in 96% ethanol (F1) and a commercially available powdered rosella extract (F2). Evaluation on the formulations demonstrated that both F1 and F2 effectively maintained the color integrity of the lip …

The Potential Of Herbal Combinations As Adjunctive Therapy For Standard Care Of Covid-19 Patients: A Lesson Learned From Indonesia, Anna Rozaliyani, Satria Pratama, Findra Setianingrum, Fenny Yunita, C. Martin Rumende

Pharmaceutical Sciences and Research

The pandemic of novel coronavirus disease (COVID-19) has become a global disaster that occurred between the end of 2019 and 2023. It caused immeasurable damage to almost all aspects of human life. The disease has opened a lot of new perspectives on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) origins and pathogenesis, the nature of the virus, the potential for new treatments, and new policies to overcome this pandemic. This review aims to know the potential of plants that are beneficial to combat the diseases, including immunomodulators in the management of COVID-19. Studies reporting COVID-19 with the keywords SARS-CoV-2, COVID-19, …

Antioxidant Activities Of Roja Herbal Tea: Combination Of Rosella Flower (Hibiscus Sabdariffa L.) And Ginger Rhizome (Zingiber Officinale Rosc.), Wahyu Widowati, Teresa Liliana Wargasetia, Teddy Marcus Zakaria, Michael Sebastian Gunadi, Nathanael Halim, Sherly Santiadi, Hanna Sari Widya Kusuma, Nida Firyal Fauziyah

Pharmaceutical Sciences and Research

Herbal teas and plant-based medicines have been widely used worldwide for their therapeutic and healing qualities. Rosella (Hibiscus sabdariffa L.) has various benefits in preventing digestive problems, liver disease, fever, and others. Ginger (Zingiber officinale Rosc.) or known as jahe in Indonesia is believed to benefit numerous clinical conditions linked to oxidative stress, including pancreatitis, hypertension, diabetic kidney disease, Alzheimer’s disease, and tumor development. They have been utilized in traditional medical practice to treat conditions such as fever, nausea, and headaches. In this study, we aimed to determine the antioxidant activities of rosella flower tea, ginger tea, and …

Study Of Anti-Hyperuricemia, Anti-Inflammatory, And Anti-Nociceptive Effects Of Hylocereus Undatus Stem Bark Extract In Animal Models, Tri Wahyuni, Sartika Harka Putri, Anton Bahtiar

Pharmaceutical Sciences and Research

Dragon fruit, known as Hylocereus undantus (HU), has traditionally been utilized in Banyuwangi, East Java, Indonesia, for addressing gout and rheumatism. Despite its empirical use, there is a lack of reported pharmacological research on this plant. This study aimed to explore the properties of HU extract in treating hyperuricemia, inflammation, and pain. Adenine-potassium oxonate-induced mice were employed to evaluate anti-hyperuricemia activity, measuring uric acid, blood urea nitrogen (BUN), and creatinine serum levels. Carrageenan-induced paw edema in rats was used to assess anti-inflammatory activity, while acetic acid-induced abdominal writhing and hot plate tests were conducted to investigate anti-nociceptive effects. The results …

Combined Risk Based Inspection And Fault Tree Analysis For Repetitive 3-Phase Line Piping Leakage At West Java Offshore Topside Facility, Dona Yuliati, Akhmad Herman Yuwono, D. Rizal Asral, Donanta Dhaneswara

Journal of Materials Exploration and Findings

Hydrocarbon releases might result in serious consequences in various aspects. In addition to the contribution to environmental pollution, repetitive leakages need high repair costs. This study aim is to minimize potential repetitive leakage for other typical 3-phase piping systems. We conducted the risk assessment by adopting Risk Based Inspection (RBI) API 581 to identify risk level, calculating piping lifetime, recommended inspection plan and mitigations. The most relevant root causes can be obtained through quantitative Fault Tree Analysis (FTA). Observation and investigation was taken from eight 3-phase piping systems that experienced repetitive leakages. It has been found that the risk level …

Nitrogen Gas Quenching Pressure Effect On Bs S155 Alloy Steel In Vacuum Furnace, Agus Mulyadi Hasanudin, Eddy Sumarno Siradj

Journal of Materials Exploration and Findings

The production of metal and alloy products requires the use of heat treatment, when during the heat treatment process, quenching is a crucial step. The quenching medium can be anything from water, a salt bath, oil, air and gas. In a vacuum furnace, pressurized gas, most frequently nitrogen (N₂) gas, serves as one of the quenching mediums. One of the drawbacks of the quenching process is the distortion and dimensional change of the parts. This paper aims to investigate the influence of nitrogen gas quenching pressure on the distortion and dimensional change of aerospace actuator gear planet parts …

Dietary Analysis For Hashimoto’S Thyroiditis: An Integrative Review, Evan Thompson, Alison Hultquist

Master of Science in Nursing Final Projects

Abstract

Hashimoto’s Thyroiditis (HT) is the leading cause of primary hypothyroidism in the United States. In HT, there is an infiltration by lymphocytes which leads to the production of autoantibodies against the thyroid gland. Throughout this integrative review, the aim was to evaluate the effectiveness of micronutrient supplementation and dietary management as adjunct treatments in HT. The purpose was to assist primary care providers in the development of a more holistic plan of care. Literature published within the past seven years was gathered and reviewed from PubMed, CINAHL, and Cochrane Library. Findings indicate that many patients with HT may benefit …

The Therapeutic Potential Of Nelumbo Nucifera: A Comprehensive Review Of Its Phytochemistry And Medicinal Properties, Sparsh Bhat, Suresh Joghee, Meenakshi S. Iyer

International Journal of Health and Allied Sciences

Nelumbo nucifera generally known as ‘sacred lotus’ is a plant with high medicinal value in traditional medicine system especially in Chinese medicine system which is having a wide distribution worldwide in Asian, Western European, American and Australian continent. All the parts of the plant have different medicinal value, as their extract contains various valuable chemical constituents which yield desired remedial effects against many diseases. The plant is used traditionally more compared to its commercial usage. It has also been used for dental and skin care products in many herbal formulations. In this review, data about its general description, its distribution, …

Piperine Encourages Apoptosis In Human Cervical Adenocarcinoma Cells Through Ros Generation, Dna Fragmentation, Caspase-3 Activation And Cell Cycle Arrest, Asif Jafri, Juhi Rais, Sudhir Kumar, Md Arshad

Research Symposium

Background: Cancer is one of the most common destructive diseases and the second leading cause of death in humans. Among cancer, cervical cancer is the second most common malignancy among women globally. Thus, there is a continuous need to search for chemotherapeutic chemicals or naturally occurring drugs to resolve this global health problem. Piperine (1-piperoylpeperdine) is present in the fruits of black pepper (Piper nigrum Linn.) and long pepper (Piper longum Linn.). It possesses several pharmacological properties and in the present study we have evaluated its anti-cancer potential on human cervical adenocarcinoma (HeLa) cells.

Methods: The anti-proliferative effect …

International Conference On Cancer Health Disparities, Juhi Rais, Asif Jafri, Neelam Shivnath, Habiba Khan, Md Arshad

Research Symposium

Background: Biochanin A, an isoflavone that is mainly present in red clover, has potent chemopreventive properties against many cancers. Ovarian carcinoma is fifth most common and deadliest gynaecological malignancy that causes the highest mortality in females worldwide. Hence a substantial need for new therapies for combating this gynaecological malignancy arises.

Methods: The present study aimed to investigate anti-cancerous potentials of biochanin A on cultured human ovarian carcinoma PA-1 cells through the cell viability assay, cellular apoptosis, disruption of mitochondrial membrane potential (MMP), involvement of ROS, cell cycle kinetics, and expression of apoptosis-related genes namely, p53, Bax, Bcl-2, Noxa and Puma. …