Bacteria Commons^™

Phanerochaete Chrysosporium Pretreatment Of Biomass To Enhance Solvent Production In Subsequent Bacterial Solid-Substrate Cultivation, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

This study investigated pretreatment of corn stover using solid substrate cultivation (SSC) of Phanerochaete chrysosporium (P. chrysosporium) to improve subsequent accessibility to cellulose. Thereafter, Clostridium thermocellum (C. thermocellum) was directly inoculated onto the pretreated biomass to accomplish hydrolysis, followed by solventogenesis by introducing Clostridium beijerinckii (C. beijerinckii).

An enzyme suite containing laccase, lignin peroxidase and manganese peroxidase activity was detected during the cultivation of P. chrysosporium on corn stover within 288 h at an initial moisture content 𝓌_H2O = 80% (mass of water/total mass). Incubation factors, such as substrate moisture content and cultivation …

The Effects That Liquid And Solid Cattle Manure Have On The Water Quality Of Drainage Ditches In Putnam County, Ohio, Janelle Horstman

Honors Projects

Lake Erie has experienced harmful algal blooms with increased frequency since the mid-1990s due to excess nutrients from Rivers, such as the Maumee River, and largely agricultural watersheds. Nonpoint source pollution from agriculture contributes to eutrophication, algal blooms, and the degradation of water quality. This creates stress on aquatic fauna, reduced aesthetic quality, odor, and limits of the water for usage of drinking, recreation, and industry. This research paper asks what the contributions of having access to manure application records, soil records, and information about antibiotics have on what is known about manure management and antibiotic resistance, which has been …

Modeling Fecal Bacteria In Oregon Coastal Streams Using Spatially Explicit Watershed Characteristics, Paul Bryce Pettus

Dissertations and Theses

Pathogens, such as Escherichia coli and fecal coliforms, are causing the majority of water quality impairments in U.S., making up ~87% of this grouping's violations. Predicting and characterizing source, transport processes, and microbial survival rates is extremely challenging, due to the dynamic nature of each of these components. This research built upon current analytical methods that are used as exploratory tools to predict pathogen indicator counts across regional scales. Using a series of non-parametric methodologies, with spatially explicit predictors, 6657 samples from non-estuarine lotic streams were analyzed to make generalized predictions of regional water quality. 532 frequently sampled sites in …

Downstream Pathway Activation In Cultured Hepatocytes Following Treatment With Francisella Tularensis, Nicole Wakefield

Undergraduate Theses and Capstone Projects

Francisella tularensis, the intracellular pathogen that causes tularemia, was investigated to determine how it infects and replicates within mammalian hepatocytes. To date, it has been shown that hepatocytes can be infected by F. tularensis and that this causes considerable change in the protein phosphorylation state of several vital signaling molecules within the host cell. Protein kinase pathways can be mapped as signatures of infection. Hepatocytes tend to be susceptible to infection by F. tularensis, thus stimulating internal signaling. The Francisella strains used were selected with the goal of producing a model that can be used to elucidate the cell signaling …

In Vitro Antimicrobial Efficacy Of Calcium Hydroxides In Root Dentin, Josef W. Lubisich

Loma Linda University Electronic Theses, Dissertations & Projects

Enterococcus faecalis is the most commonly isolated bacteria in failed root canal treatment. Endodontic intracanal medicaments are commonly tested using standardized bovine cylinders infected with bacteria. The literature is not clear on whether calcium hydroxides are able to remove E. faecalis from the dentinal tubules. One reason for this is that there are several varying forms of calcium hydroxide commonly used. The purpose of the present experiment was to determine whether four commonly used calcium hydroxide products were able to reduce E. faecalis from four incremental samples of dentin and to determine if there was a statistically significant difference between …

Comparing The Use Of A Manual And A Power Toothbrush By 3-To-4 Year-Old Children, Lorena Delgado Salcedo

Loma Linda University Electronic Theses, Dissertations & Projects

PURPOSE: To compare the ability of three-to four-year-old children in performing toothbrushing with manual versus battery-powered toothbrushes with and without oral hygiene instructions.

METHODS: This study was a randomized, controlled, examiner-blind, 4-period crossover design performed on 50 healthy children, ages 3 to 4 years old. Children were assigned either a manual or power toothbrush at visit one and alternated between the two types of toothbrushes each week, for four weeks. Plaque was scored before and after brushing using the Greene and Vermillion -Simplified Oral Hygiene Index. ^18,19 At their last two visits of the study, the children received brushing instructions …

A Pilot Study Testing A Proprietary Sealant For Plaque Reduction, Jennifer Rowland

Loma Linda University Electronic Theses, Dissertations & Projects

Enamel demineralization due to increased plaque accumulation is a well-recognized problem associated with fixed orthodontic appliances. The purpose of this study was to evaluate the efficacy of a proprietary product to be marketed by 3M Unitek for the reduction of plaque accumulation and thereby reduce enamel demineralization.

Sixty four bovine teeth were divided equally into an untreated control group and a group treated with the proprietary sealant product. Both groups were subsequently immersed in a Streptococcus mutans culture. Plaque deposits from each group were removed, suspended in trypticase soy broth, serially diluted, and plated on trypticase soy agar at 1, …

Indicator Bacteria Concentrations Of Two Northwest Arkansas Streams In Relation To Flow And Season, Dwayne R. Edwards, Mark S. Coyne, Tommy C. Daniel, P. F. Vendrell, J. F. Murdoch, P. A. Moore Jr.

Biosystems and Agricultural Engineering Faculty Publications

Concentrations of indicator bacteria such as fecal coliform (FC) and fecal streptococcus (FS) are often used to assess the suitability of waters for their intended use(s) and to allocate resources for water quality improvement measures. There is evidence, however, that concentrations of FC and FS can be influenced by variables such as season and flow rate during sampling, which could lead to biased results. The objective of this study was to assess the impacts of season and flow rate on concentrations of FC and FS. Fecal indicator bacteria concentrations were measured for approximately three years at five sites on two …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …

The Determination Of Antibody Levels In Endodontic Patients Against Eleven Microorganisms Using Ifa And Elisa Techniques, William C. Payne

Loma Linda University Electronic Theses, Dissertations & Projects

Endodontic (root canal) infections are generally considered to be caused by the invasion of microorganisms which destroy pulp tissue and extend to the periapical region. No definitive study has identified a specific bacterium or group of bacteria as etiological agents. We quantitively measured antibodies present in the serum of 30 normal individuals and patients with either chronic (n = 30) or acute (n = 30) periapical lesions with the indirect fluorescent antibody (IFA) and enzyme-linked immunosorbent assay (ELISA) for IgG and IgM against 11 oral microorganisms routinely isolated from such lesions. The results were analyzed statistically. Analysis of variance of …

Movement Of Bacteria Through Macropores To Ground Water, M. Scott Smith, Grant W. Thomas, Robert E. White

KWRRI Research Reports

Effects of soil type, flow rate, antecedent soil moisture and other factors on transport of E. coli through soils was measured on disturbed and intact columns 20 cm in diameter by 25 to 30 cm in depth. Added E. coli were distinguished from indigenous microbes using an antibiotic resistance marker. Transport of Cl^- and ³H₂O was also measured. Up to 96 percent of the bacteria irrigated onto the surface of intact columns were recovered in the effluent. Soil structure appeared to be related to the extent of transport. Columns prepared from mixed, repacked soil were much …

Summary Of Medic-Rhizobium Field Experiments, J G. Howieson

Experimental Summaries - Plant Research

83ME9 - Nutrition of R. meliloti in acid soil. - Meaningful results only available in 1984/85 Summary of Medic-Rhizobium Field Experiments 1983/84. 83ME87 - Effect of pH of culture conditions on survival in, and colonisation of, an acid soil by R. meliloti. - Meaningful results available 1984/85. (82ME22, 82ME22A, 82ME23) Survival and colonisation of acid soils by 18 strains of R. meliloti in association with 6 Medicago hosts. - Results discussed in this summary.

Petroleum-Utilizing Bacteria: The Biological Line Defense Against Oil Pollution, Steven W. Sokolowski

Biological Sciences Theses & Dissertations

A silica gel-petroleum (SGP) medium was developed which is reliable, approximately the same cost as that of the corresponding agar-based petroleum medium, and can be made sufficiently firm to use in streaking or the spread-plate technique. Advantages of the medium include: the incorporation of all of the major ions of seawater in accordance with Dittmar's Law (the salinity of which can easily be varied from 0 to 35 parts per thousand); a wide workable pH range (5.5 to 11.6); negligable pH drift(~ 0.2 pH units after 166 days); the elimination of all but negligable amounts of syneresis, the ability to …

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

Metabolic Role Of Sulfates And Sulfides Producing Bacteria In Pollution Of Waters, M. I. H. Aleem

KWRRI Research Reports

Cytochrome transport particles from Nitrobacter catalyzed nitrite, ascorbate as well as NADH oxidation with concomitant phosphate esterification yielding P/0 ratios of 1.0, 0.6 and 2.0 respectively. Phosphorylation coupled to nitrite oxidation was not effected by rotenone, amytal or antimycin while 50 and 70% inhibition of the NADH-linked phosphorylation was observed in the presence of HOQNO and rotenone respectively. Cell-free extracts from Nitrobacter also catalyzed an energy-dependent reduction of NAD⁺ by nitrite. The reduction of cytochrome c by NŌ₂ was energy-dependent which involved the reversal of electrons from cytochrome a₁. The subsequent energy-linked reduction of the flavoproteins …

Algal Growth And Decomposition: Effects On Water Quality, Edward G. Foree, John S. Tapp Jr.

KWRRI Research Reports

The chemical composition of algae grown in batch culture depends mainly on environmental conditions, nutrient availability, presence of predators, cell age, and species. The effects of nutrient availability and cell age on the composition of three unialgal cultures (algae + bacteria) and one hetergeneous culture (algae + bacteria + microscopic animals) were evaluated. The cultures were grown in batch culture under both nutrient-abundant and nutrient deficient conditions and the changes in compositions were observed. Luxurious uptake where nutrients are incorporated into cellular protoplasm at levels greater than those necessary for growth, and super-luxurious uptake, where some nutrients are stored rather …