Organisms Commons^™

First Proof Of Concept Of Sustainable Metabolite Production From High Solids Fermentation Of Lignocellulosic Biomass Using A Bacterial Co-Culture And Cycling Flush System, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

To improve the lignocellulose conversion for ABE in high solids fermentation, this study explored the feasibility of cycling the process through the cellulolytic or/and solventogenic phases via intermittent flushing of the fermentation media. Five different flushing strategies (varying medium ingredients, inoculum supplement and cycling through phases) were investigated. Flushing regularly throughout the cellulolytic phase is necessary because re-incubation at 65 °C significantly improved glucose availability by at least 6-fold. The solvents accumulation was increased by 4-fold using corn stover (3-fold using miscanthus) over that produced by flushing only through the solventogenic phase. In addition, cycling process was simplified by re-incubating …

Silence Is The Loudest Sound, Emma Christian

Honors College

Rhinoceros poaching is an act of killing a rhinoceros in order to take its horn, which is then used for human consumption or for cultural traditions. Both the Asian and the African rhinoceros are targets because of the demand from China, Vietnam, Yemen, and other countries around the world. Traditional Chinese Medicine practice is the main demand for rhinoceros horn and this demand in rhinoceros horn has caused an increase in the black market. Conservation of the rhinoceros is decreasing primarily because the price of rhinoceros horn is more than double the average household income in South Africa, thus making …

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO₂ capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO₂ source. Algae productivity of 39 g/(m² day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m² day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …

Phanerochaete Chrysosporium Pretreatment Of Biomass To Enhance Solvent Production In Subsequent Bacterial Solid-Substrate Cultivation, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

This study investigated pretreatment of corn stover using solid substrate cultivation (SSC) of Phanerochaete chrysosporium (P. chrysosporium) to improve subsequent accessibility to cellulose. Thereafter, Clostridium thermocellum (C. thermocellum) was directly inoculated onto the pretreated biomass to accomplish hydrolysis, followed by solventogenesis by introducing Clostridium beijerinckii (C. beijerinckii).

An enzyme suite containing laccase, lignin peroxidase and manganese peroxidase activity was detected during the cultivation of P. chrysosporium on corn stover within 288 h at an initial moisture content 𝓌_H2O = 80% (mass of water/total mass). Incubation factors, such as substrate moisture content and cultivation …

Regional Dust Storm Modeling For Health Services: The Case Of Valley Fever, William A. Sprigg, Slobodan Nickovic, John N. Galgiani, Goran Pejanovic, Slavo Petkovic, Mirjam Vujadinovic, Ana Vukovic, Milan Dacic, Scott Dibiase, Anup K. Prasad, Hesham El-Askary

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

On 5 July 2011, a massive dust storm struck Phoenix, Arizona (USA), raising concerns for increased cases of valley fever (coccidioidomycosis, or, cocci). A quasi-operational experimental airborne dust forecast system predicted the event and provides model output for continuing analysis in collaboration with public health and air quality communities. An objective of this collaboration was to see if a signal in cases of valley fever in the region could be detected and traced to the storm - an American haboob. To better understand the atmospheric life cycle of cocci spores, the DREAM dust model (also herein, NMME-DREAM) was modified to …

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO₂ mitigation strategy is the use of microalgae for mitigation of CO₂. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO₃ based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO₃ based media. …

Influence Of Flue Gas Components On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus, Czarena Crofcheck, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to the increase in combustion of fossil fuels. One possible alternative strategy is the use of microalgae for CO₂ capture and recycling. Major components in coal-derived flue gas that may accumulate and effect algae growth include both sulfur oxides and fly ash. However, in practical application, sulfur oxides will be converted quickly to the acid product (H₂SO₄) in the aerobic aqueous conditions of algae cultivation. In this article, the influence of elevated H₂SO₄ levels and the presence of coal-derived fly ash …

Cyanobacteria Dominance In The Oligohaline Waters Of Back Bay, Virginia, Harold G. Marshall

Biological Sciences Faculty Publications

Back Bay and its flora have historically been influenced by the interaction of freshwater flow in combination with frequent intrusion of saline water into its basin. These events have resulted in a dynamic environmental setting influencing the abundance and composition of its phytoplankton community. Dominating these oligohaline waters is a diverse representation and high abundance of freshwater filamentous and colonial cyanobacteria. These include the nonheterocystous Planktolyngbya contorta, Planktolyngbya limnetica, and Pseudanabaena limnetica, taxa implicated as bloom producers in Bay waters with N:P molar ratios ranging from 23:1 to 74:1.

The Transition Zone: Impact Of Riverbanks On Emergent Dragonfly Nymphs. Implications For Riverbank Restoration And Management, Kirsten Hope Martin

Antioch University Full-Text Dissertations & Theses

The use of riprap in the restoration and stabilization of riverine landscapes is an issue of concern for many ecologists. While current methods of bank stabilization, especially those involving the placement of rocks (riprap) along the waterline, are effective in controlling erosion their presence changes habitat components (slope, substrate composition, near-shore river velocity) at the river-land interface. The additional impacts of river current, water temperature, soil composition, slope, and water level fluctuation, may further imperil emerging nymphs. The purpose of this research is to document the effects of riprap, location (upriver or downriver of hydroelectric intake/outtake facilities), water level fluctuation, …

Liquid Chromatography-Tandem Mass Spectrometry For The Determination Of Methylprednisolone In Rat Plasma And Liver After Intravenous Administration Of Its Liver-Targeted Dextran Prodrug, Shuang-Qing Zhang, Helen R. Thorsheim, Suman Penugonda, Venkateswaran C. Pillai, Quentin R. Smith, Reza Mehvar

Pharmacy Faculty Articles and Research

A specific and sensitive liquid chromatography (LC)-tandem mass spectrometric method for quantitative determination of methylprednisolone (MP) in rat plasma and liver was developed and validated using triamcinolone acetonide as an internal standard. Liquid-liquid extraction using tert-butyl methyl ether was used to extract the drug and the internal standard from plasma and liver. The separation of MP was performed on a C(18) column with a mobile phase of acetonitrile:0.5% formic acid aqueous solution (85:15, v/v) over 4 min. The assay was based on the selected reaction monitoring transitions at m/z 375 -> 161 for MP in plasma, 375 -> 357 for …

Phytoplankton Composition Within The Tidal Freshwater-Oligohaline Regions Of The Rappahannock And Pamunkey Rivers In Virginia, Harold G. Marshall, Lubomira Burchardt

Biological Sciences Faculty Publications

The Rappahannock River is a major river system across north central Virginia prior to entering the Chesapeake Bay. In contrast, the Pamunkey River is smaller in size and joins the Mattoponi River to form the York River, which flows parallel to the Rappahannock before it also flows into Chesapeake Bay. A unique mixing area for both flora and environmental conditions exists in the tidal freshwater-oligohaline region of both rivers. This is a dynamic mixing section where freshwater and estuarine species are subject to the interaction of river flow and daily tidal rhythms. The phytoplankton composition in this region of the …

An Endophytic Fungus As A Source Of New Antifungal Compounds, Lisa Marie Jungbauer

Honors Theses, 1963-2015

Medical advances in society such as organ transplants, prolonged chemotherapy, and those that lengthen the lives of AIDS patients and the elderly increase the number of immunocompromised individuals(1). When the immune system is compromised, opportunistic fungi can flourish and become fatal. Current antifungal treatments are limited and often toxic(2,3). In addition, strains of fungi resistant to available antifungals are emerging(4,5). Fungi were selected as the source of potential new antifungal agents because fungal antagonism has been reported in most fungal ecosystems(6). Endophytic fungi, which inhabit the spaces between plant cells, are known producers of natural products and that assist plants …

Indicator Bacteria Concentrations Of Two Northwest Arkansas Streams In Relation To Flow And Season, Dwayne R. Edwards, Mark S. Coyne, Tommy C. Daniel, P. F. Vendrell, J. F. Murdoch, P. A. Moore Jr.

Biosystems and Agricultural Engineering Faculty Publications

Concentrations of indicator bacteria such as fecal coliform (FC) and fecal streptococcus (FS) are often used to assess the suitability of waters for their intended use(s) and to allocate resources for water quality improvement measures. There is evidence, however, that concentrations of FC and FS can be influenced by variables such as season and flow rate during sampling, which could lead to biased results. The objective of this study was to assess the impacts of season and flow rate on concentrations of FC and FS. Fecal indicator bacteria concentrations were measured for approximately three years at five sites on two …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …

Long Season Wheat, W. R. Smith, K. Anderson

Experimental Summaries - Plant Research

Agronomy of long season wheats, 87AL9, 87AL10, 87ES3, 87ES4, 87JE2, 87JE3, 87MT8, 87MT9 and 87NA9. Septoria control in long season wheat, 87E4 and 87MT10. Nitrogen response by long season wheat, 87ES5 and 87JE4. Phenology of long season wheats, 87E5 and 87MT11.

Grain Legumes Evaluation., G. H. Walton, T. R. Trent

Experimental Summaries - Plant Research

Lupin agronomy, 87AL14. Nitrogen fertilizer for legume crops, 87BA2. Pea variety evaluation, 87C59, 87M08, 87ME1, 87N012, 87SG8, 87KA7, 87N2. Interstate pea variety trials, 87N096, 87N099, 87KA6. Disease Resistance Pea Variety Testing, 87JE1. Grain legume species comparisons, 87A2, 87C2, 87KA37, 87M09, 87MA1, 87NA15. Legume species variety trials, 87LG2. Faba bean evaluation, 87MC34 and 87KA8. Faba bean 'synthetic' variety yield trial, 87MC36. Faba bean screening nursery, 87MC35. Preliminary agronomy of faba bean, chickpea and lentil, 87A21. Seeding date, 87A22. Legume species herbicide tolerance, 87KA82.

High Input Barley Production Systems In The High Rainfall Zone., G. J. Parlevliet

Experimental Summaries - Plant Research

Barley systems in the high rainfall zone effect of nitrogen fungicides and seeding rate on barley yield, 87BR2.

Fungicide Testing, M. J. Barbetti

Experimental Summaries - Plant Research

Fungicidal control of Phoma blackstem disease in medic, 86 KA 11. Medic cultivar susceptibility to Phoma blackstem disease, 85 KA 56. Role of Phoma seed infection in medic, 85 KA 57. Effect of soil pasteurization on damage caused by six fungal root pathogens, 86 PE 53. Susceptibility of 18 sub-clover cultivars to isolates of the clover scorch fungus, Kabatiella caulivora, 86 PE 54. Effect of plant age or growth stage of subterranean clover on susceptibility to infection by the clover scorch fungus, Kabatiella caulivora, 86 PE 55. Susceptibility of rapeseed lines to blackleg crown canker, 86 MT 1, 86 MT …

Foliar Wheat Diseases And Cereal Smuts., R. Loughman, A. E. Twigg

Experimental Summaries - Plant Research

Chemical control of leaf spots of wheat, 86BA41, 86MT49. Semi-natural inoculum for plant breeders plots, 86MD2. Chemical control of soil-borne flag smut, 86ME73, 86M032. Chemical control of barley loose smut, 86AL50, 86KA59, 86MT47. Chemical control of loose smut in barley with different levels of seed infections, 86MT48. Disease characterisation of cereal variety trial stage 4 sites.

Lupin Root Rot, Lupin Hypocotyl Rot, Rhizoctonia Coleoptile Rot Of Wheat, Rhizoctonia Root Rots In Long Term Wheat-Pasture Rotation Trials., M. Sweetingham

Experimental Summaries - Plant Research

Lupin Root Rot: 86Cl4, 86WH15, 86ME85, 86LG63, 85C63, 86WH14. Lupin Hypocotyl rot, 86BA49, 86BA21. Rhizoctonia coleoptile rot of wheat, 86BA19, 86BA20 Rhizoctonia root rots in long term wheat-pasture rotation trials.

Effect Of Pasture Spraying On Gall Development., V. Nieman

Experimental Summaries - Plant Research

Sprays with low rates of desiccant herbicide just after head emergence has been shown to control development of toxicity in the same year. The trial was conducted to compare the effect of herbicides with different modes of action on control of toxicity and seed set. 85KA65.

Disease Rating System., M Sweetingham

Experimental Summaries - Plant Research

Lupin hypocotyl rot trials, 85BA29, 85BA30, 85BA31, 85N054. Lupin root rot trials, 85WH31, 85WH32, 85WH52. Lupin root disease survey - Moora District. Common Root Rot studies, 82M26.

Studies On The Effects Of Nutrition And Tillage Systems On Cereal Root Diseases., R. F. Brennan

Experimental Summaries - Plant Research

Micronutrients (Cu, Zn) and take-all, 83ES39, 83ES40. Nitrogen and Phosphorus rates and take-all, 83ES41, 83ES42, 83ES43. Split applications of Nitrogen and take-all, 84N1. Take-all and Manganese/Ammonium interaction, 84E1. Split applications of Nitrogen and take-all, 84E2. Cu residual on wheat and take-all, 67E9, 67E8. Take-all and Manganese/Ammonium interaction, 84LG2. Cultivation depths and timing effects on Rhizoctonia, 84E24. Chemical fallow and cultivation effects on Rhizoctonia, 85E33. Effects of cultivation depths and machines on Rhizoctonia and yield, 85E29.

Root And Foliar Diseases Of Wheat On Sandplain In The Geraldton Region., J. Wilson

Experimental Summaries - Plant Research

A project funded by Wheat Industry Research Council and Wheat Industry Research Committee of W.A. Interactions between stubble, rotation, ripping and nitrogen, ECRS, 85C53. The effect of type of cultivation implement, and time of cultivation, ECRS, 85C54. The effect of fumigation, ECRS, 85C91. Residual ripping responses from 1984, 84C15.

Barley Agronomy., K. J. Young, B. R. Wylde, B. Raszyk, T. N. Khan

Experimental Summaries - Plant Research

Barley foliar diseases, effect on nitrogen response, 85ES25, 85E24, 85E25, 85MT37, 85ES29, 85MT38, 85E26, 85ES32, 85ES31, 85ES30, 85ES26, 85ES24, 85ES27, 85ES28, 85ES34, 85M27, 85SG27, 85MT42, 85C70,

Barley Foliage Diseases, Field Pea Diseases., T. N. Khan, K. Brain, M. Judges, K. J. Young, I. Pritchard, T. Sweeny, J. Hamblyn

Experimental Summaries - Plant Research

85BA22, 85MT31, 85N053, 85BA24, 85MT32, 85MT32, 85N052, 85N053, 85BA21, 85MT30, 85BA21, 85BA22, 85MT31, 85MT43, 85KA59, 85KA60, 85M29, 85MT44, 85E22, 85ES22, 85ES23, 85N051, 85BA25, 85WH27, 85C65, 85C69, 85C66, 85C67, 8SKA48, 8SMT34, 85KA47, 85MT33, 85M26, 85ME26, 85KA49, 85KA51.

A.General Notes and Highlights

BARLEY FOLIAGE DISEASES

B. Scald: Screening fungicides

c. Scald: Fungicidal control in Northam district

D. Scald: Effect of seeding rate

E. Scald & Mildew Effect of fungicides and rates

F. Scald & Mildew Seed borne infection and seed dressing

G. Scald & Mildew Effect of seed dressing and spraying

H. Scald & Mildew Effect of Erex and Baytan

I. Mildew: …

Low Tillering Cereals, Early Maturity Of Cereals, Cereal Variety And Reduced Branching Lupins., R. Delane, J. Hamblin

Experimental Summaries - Plant Research

Trial 85C62 - growth and water use of low tillering cereals. Trial 85C62 - yield potential of low tillering cereals. Trial WEUNI - response of low tillering breeding lines and standard varieties to sowing rate. Trial 85C45 - effect of flowering date, seeding rate, deep tillage and applied nitrogen on yield of barley. Trial 85C61 - wheat variety x management interaction on sandplain. Trial 85C55 - growth and water use of reduced branching lupins. Trial 85C56 - normal and reduced branching lupins - planting density response.

Effect Of Soil Type On Relative Crop Yields, Effect Of Seeding Time On Pea And Lupin Yields, Effect Of Seeding Rate On Field Pea Yields, Studies In Legume Nodulation And Response Of Cereals In The Year Following Legumes., R. French

Experimental Summaries - Plant Research

Trials 85M36, 85M37, 85M38, 85M39. Trials 8SJE17, 85KA62, 85N20, 85ME42, 85ME43, 85ME44, 85WH48. Trials 85KA63, 85M33. Trials 85M32, 85M34, 85M35. Trials 84M50, 84M51, 84M52.

Cercospora Disease Of Subterranean Clover, Root Rot, Cereal Smuts, Rapeseed Diseases, M J. Barbetti

Experimental Summaries - Plant Research

Cercospora disease of subterranean clover: - . Susceptibility to and losses from cercospora on sub-clover cultivars. Locality: Bibby Springs, Cervantes. (84M073, 84M075, 84M076). Subterranean clover root rot: - Seed treatments for root rot control. Locality: Albany (84AL39); Scott River (84 BU11). Fungicide drenches for root rot control. Locality: Perth (84PE22) using intact cores from Albany and Scott River. Cultivar screening for resistance to root rot fungi. Locality: Perth (84PE24) with field plantings at Albany and Scott River. Methyl bromide fumigation for control of sub-clover root rot. Locality: Vasse Research Station (84V15). Two sites. Cereal smuts – Fungicidal control of loose …

Studies On The Effects Of Nutrition And Tillage Systems On Cereal Root Diseases, R F. Brennan

Experimental Summaries - Plant Research

1. Split Applications of Nitrogen and Take-all 84N1. 2. Split Applications of Nitrogen and Take-all 84E2. 3. Split Applications of Nitrogen and Take-all 84MT4. 4. Split Applications of Nitrogen and Take-all 84LG1. 5. Nitrogen and Phosphorus Rates and Take-all 83ES41. 6. Nitrogen and Phosphorus Rates and Take-all 83ES42. 7. Nitrogen and Phosphorus Rates and Take-all 83ES43. 8. Micronutrients (Cu, Zn) and Take-all 83ES39. 9. Micronutrients (Cu, Zn) and Take-all 83ES40. 10. Cu Residual on Wheat and Take-all 67E9. 11. Cu Residual on wheat and Take-all 67E8. 12. Take-all and Manganese/Ammonium Interaction 84LG2. 13. Take-all and Manganese/Ammonium Interaction 84MT5. 14. Take-all …