Organisms Commons^™

Summary Of Medic-Rhizobium Field Experiments, J G. Howieson

Experimental Summaries - Plant Research

83ME9 - Nutrition of R. meliloti in acid soil. - Meaningful results only available in 1984/85 Summary of Medic-Rhizobium Field Experiments 1983/84. 83ME87 - Effect of pH of culture conditions on survival in, and colonisation of, an acid soil by R. meliloti. - Meaningful results available 1984/85. (82ME22, 82ME22A, 82ME23) Survival and colonisation of acid soils by 18 strains of R. meliloti in association with 6 Medicago hosts. - Results discussed in this summary.

The Absence Of Extrachromosomal Dna In Methanogenic Bacteria, Nancy Ann Kurkinen

Dissertations and Theses

Five species of methanogenic bacteria were analyzed for the presence of plasmid DNA. Several procedures for the detection and isolation of covalently closed circular plasmid DNA were modified for use with the methanogens.

Petroleum-Utilizing Bacteria: The Biological Line Defense Against Oil Pollution, Steven W. Sokolowski

Biological Sciences Theses & Dissertations

A silica gel-petroleum (SGP) medium was developed which is reliable, approximately the same cost as that of the corresponding agar-based petroleum medium, and can be made sufficiently firm to use in streaking or the spread-plate technique. Advantages of the medium include: the incorporation of all of the major ions of seawater in accordance with Dittmar's Law (the salinity of which can easily be varied from 0 to 35 parts per thousand); a wide workable pH range (5.5 to 11.6); negligable pH drift(~ 0.2 pH units after 166 days); the elimination of all but negligable amounts of syneresis, the ability to …

The Development Of Techniques For The Enumeration Of Selected Pathogenic Bacteria In Lynnhaven Estuarine Sediments, Mark T. Frank

Biological Sciences Theses & Dissertations

Methodologies for the improved recovery of selected pathogenic bacteria from estuarine sediments were investigated. Laboratory and field studies were conducted to document the sensitivity of the techniques for isolating Salmonella species, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. Vibrio parahaemolyticus-like organisms were enriched in a seawater yeast extract broth and isolated from Thiosulfate Citrate Bile Salts Sucrose plated media. Enrichment procedures with and without elevated temperatures and subsequent transfers to selective and differential plated media have been examined for the isolation of Salmonella species and P. aeruginosa. These pathogens were initially assayed for utilizing primary enrichments of asparagine …

Gingival Condition In Areas Of Minimal And Appreciable Widths Of Keratinized Gingiva, Myles C. Miyasato

Loma Linda University Electronic Theses, Dissertations & Projects

This investigation evaluated the gingival condition of areas with minimal (less than or equal to 1.0 millimeter) and appreciable (greater than or equal to 2.0 millimeters) widths of keratinized gingiva.

Sixteen dental, dental hygiene, and dental assisting students and dental faculty who had contralateral or unilateral areas of minimal and appreciable widths of keratinized gingiva on the mid-buccal surface of mandibular bicuspids were examined. Gingival exudate, plaque, gingival inflammation based on color change and/or swelling and bleeding on probing were evaluated. The results showed that all areas examined were plaque free. Three of 32 areas showed clinical gingival inflammation and …

The Bacteriology Of The Biliary Tract: A Preliminary Report., J. A. Sapala, J. L. Ponka, T. R. Neblett

Henry Ford Hospital Medical Journal

The bacterial flora of acute biliary tract disease has not been completely elucidated. The organisms identified most often were coliforms. Anaerobic culture technics were not precise enough to provide proper identification. In a 12-month study of 250 patients operated upon for inflammatory gallbladder disease, culture specimens were collected in a most strict and rigorous fashion. It was hoped that a more exact understanding of the relationship between bacteria and inflammatory biliary tract disease might be appreciated. Important technics were developed for gathering specimens, delivery to the laboratory and culturing. More meaningful results are to be expected In the future.

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

The Lasting Antimicrobial Qualities Of Three Root Canal Sealing Materials : Grossman's Sealer, Rickert's Sealer, And N2 Permanent, Lee R. Ingersoll

Loma Linda University Electronic Theses, Dissertations & Projects

An in vitro study using polyethylene tubes filled with Grossman's sealer, Rickert's sealer, and N2 permanent was conducted over a six month period in an effort to determine their lasting antimicrobial properties against eight different microorganisms.

From the study it was observed that N2 permanent possesses a long-lasting antimicrobial effect, but this effect appears to diminish greatly with time. Further investigation is needed to determine whether or not the effect is actually permanent in nature. The N2 permanent was also shown to have broader antimicrobial effectiveness against the eight microorganisms tested. N2 permanent produced zones of inhibition against five of …

Experimentation To Develop Procedures To Be Used In The Investigation Of The Effects Of Restriction On The Unmodified Dna Of The Bacteriophage Lamda, John Haynes

Honors Theses

The results of experimentation approximately twenty years ago with variuos bacteriophages by Ralston and Krueger¹⁶, Anderson and Felix¹, Luria and Human¹², and by Bertani and Weigle² have shown that the host range of a given phage depends directly on the bacterial strain on which the phage has last propagated. This event came to be known as host-induced modification or host-controlled variation. As this phage host range is controlled by the host environment and can be altered from one generation to another it has, according to Luria, made microbiology "the last stronghold of Lamarckism" …

Proteolytic Enzymes Of Lactic Streptococci And Their Use In Improving Body And Texture Of Direct Acid Cottage Cheese, Gulamnabi Y. Vahora

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Proteolytic activity of Streptococcus cremoris HP, P₂ and AM₂; Streptococcus lactis-C₂, ML₈ and a commercial mixed culture was determined in skimmilk and whey by measuring the increase in Absorbency at 274.5 nm of a pH 4.6 soluble filtrate, following incubation in a 2.5% casein solution. Proteolytic activity, rate of acid production and growth rate decreased in order of C₂, HP, mixed culture, AM₂, ML₈ and P₂ at a regulated pH (6.1 - 6.2) and 32 C. Maximum proteolytic activity for all cultures was found during the stationary …

Metabolic Role Of Sulfates And Sulfides Producing Bacteria In Pollution Of Waters, M. I. H. Aleem

KWRRI Research Reports

Cytochrome transport particles from Nitrobacter catalyzed nitrite, ascorbate as well as NADH oxidation with concomitant phosphate esterification yielding P/0 ratios of 1.0, 0.6 and 2.0 respectively. Phosphorylation coupled to nitrite oxidation was not effected by rotenone, amytal or antimycin while 50 and 70% inhibition of the NADH-linked phosphorylation was observed in the presence of HOQNO and rotenone respectively. Cell-free extracts from Nitrobacter also catalyzed an energy-dependent reduction of NAD⁺ by nitrite. The reduction of cytochrome c by NŌ₂ was energy-dependent which involved the reversal of electrons from cytochrome a₁. The subsequent energy-linked reduction of the flavoproteins …

The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay

Honors Theses

Induction of Entner-Duodoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but not glucose, induced synthesis of gluconokinase and 6-phosphogluconate dehydratase in both mutants. High constitute levels of 2-keto-3-deoxy-6-phosphogluconate aldolase were found when both mutants were grown on glucose. Growth of parent and both mutant strains on glycerol also resulted in high levels of Entner-Doudoroff pathway enzymes. It was concluded …

The Duration Of The Effect Of Different Carbohydrates On Neutrophilic Phagocytosis, Judy L. Reeser

Loma Linda University Electronic Theses, Dissertations & Projects

In order to determine the duration of the effect of glucose, fructose, sucrose, honey, starch, and orange juice on neutrophilic phagocytosis, 10 subjects underwent a complete series of carbohydrate tolerance tests. Blood samples were drawn at specific time periods following ingestion of 100 grams of each carbohydrate. Blood was then analyzed for glucose and phagocytic index (the mean number of bacteria engulfed per leukocyte); a red blood cell and white blood cell count and differential white cell count were done also on about 1/2 of the samples. In addition, 7 other subjects participated in a fasting study in which no …

Molecular Weight Determinations Of Some Salmonella Potsdam Bacteriophage Dna's, Winston H. Richards

Loma Linda University Electronic Theses, Dissertations & Projects

Two group A phages, P9c/2 and P4/2, were studied. Their density, sedimentation velocity, and biochemical properties were compared. Their densities were determined by density gradient centrifugation in 38.3% cesium chloride. Sedimentation velocity was used to determine their molecular weights. To accomplish this, the DNA of P9c/2 and P4/2 was labeled with tritium and T₂ DNA, labeled with ³²P, was used as a standard. The DNA of P9c/2 and P4/2 was separately mixed with T₂ DNA and centrifuged in a 0-15% sucrose gradient. The molecular weights were calculated using the formula D₂/D₁= (M_{2 …}

Algal Growth And Decomposition: Effects On Water Quality, Edward G. Foree, John S. Tapp Jr.

KWRRI Research Reports

The chemical composition of algae grown in batch culture depends mainly on environmental conditions, nutrient availability, presence of predators, cell age, and species. The effects of nutrient availability and cell age on the composition of three unialgal cultures (algae + bacteria) and one hetergeneous culture (algae + bacteria + microscopic animals) were evaluated. The cultures were grown in batch culture under both nutrient-abundant and nutrient deficient conditions and the changes in compositions were observed. Luxurious uptake where nutrients are incorporated into cellular protoplasm at levels greater than those necessary for growth, and super-luxurious uptake, where some nutrients are stored rather …

The Overwintering Of The Fireblight Bacteria Outside Of Living Tissue In Utah, Sherman V. Thomson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this investigation was to determine the sites of overwintering of Erwinia amylovora (Burrill) Winslow et al. outside of living tissue in Utah. Bacterial isolates were taken from the surfaces of blighted twigs and mummified fruits remaining on the tree; also from internal dead tissue of blighted twigs and mummies, and from soil beneath blighted trees. Each isolate was tested for agglutination with antiserum specific for Erwina amylovora, susceptibility to Erwinia amylovora bacteriophage, production of symptoms in pear seedlings and development of bacterial ooze on green pears.

Both virulent and avirulent isolates were taken from all sites tested. …

Mastitis : Prevention And Control. 3. Implementing The Mastitis Control Programme, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

FARMERS who have followed the first two articles in this series should by now have established the recommended milking routine and be familiar with it.

A Survey Of Typical And Atypical Mycobacteria Isolated From Human Specimens, Joseph Paul Truant

Henry Ford Hospital Medical Journal

No abstract provided.

Unusual Bacterial Isolates Obtained From A Variety Of Clinical Specimens, J. P. Truant

Henry Ford Hospital Medical Journal

No abstract provided.

Absence Of Pasteurella Pestis In The Wildlife Of Arkansas, R. Reece Corey, Leo J. Paulissen, Delbert Swartz

Journal of the Arkansas Academy of Science

No abstract provided.

A Study Of The Bacteriological Effectiveness Of Oral Thermometer Procedures In Three Selected Hospitals, Esther Mae Rose

Loma Linda University Electronic Theses, Dissertations & Projects

Because pathogens such as streptococcus and staphylococcus have been transferred in many hospitals, the source of the infections is of concern to doctors, nurses, and other medical personnel. As shown by the review of literature, good techniques are necessary to stop the spread of disease.

It was the purpose of this study to investigate one facet of patient safety by: (1) the culture of thermometers ready for use in three selected hospitals; (2) to determine the effectiveness of the Group, Individual, and Central Supply methods of disinfection currently practiced in the three selected hospitals.

The method for the experiment of …

An Evaluation Of Two Preoperative Skin Preparation Procedures Used In The Operating Room Of A Selected Hospital, Maxine Darling

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to find out the immediate and residual antibacterial effects of the two preoperative skin preparation procedures most commonly used in the operating room of this selected hospital.

This study was conducted on one hundred selected surgical patients from the general medical and surgical units of this hospital. The preoperative skin preparation Procedure A (ether-alcohol-tincture of zephiran) was compared with the preoperative skin preparation Procedure B (3 per cent hexachlorophene solution). Data for the comparison were gathered by taking four cultures at specified times. The cultures were taken from the operative sites on the surgical …

The Staphylococci, Joseph Paul Truant

Henry Ford Hospital Medical Journal

No abstract provided.