Pathology Commons^™

Clinical Evaluation Of Three Sample-To-Answer Platforms For The Detection Of Sars-Cov-2, W Zhen, E Smith, R Manji, D Schron, Gj Berry

Journal Articles

No abstract provided.

Analytical And Clinical Comparison Of Three Nucleic Acid Amplification Tests For Sars-Cov-2 Detection., E Smith, W Zhen, R Manji, D Schron, S Duong, Gj Berry

Journal Articles

No abstract provided.

3d Printed Alternative To The Standard Synthetic Flocked Nasopharyngeal Swabs Used For Covid-19 Testing., S. J. Decker, T. A. Goldstein, J. M. Ford, M. N. Teng, R. S. Pugliese, G. J. Berry, O. Bloom, D. A. Breining, J. Crawford, K. Kim, +12 Additional Authors

Journal Articles

BACKGROUND:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, can be detected in respiratory samples by Real-time Reverse Transcriptase (RT)-PCR or other molecular methods. Accessibility of diagnostic testing for COVID-19 has been limited by intermittent shortages of supplies required for testing, including flocked nasopharyngeal (FLNP) swabs. METHODS:We developed a 3D-printed nasopharyngeal (3DP) swab as a replacement of the FLNP swab. The performance of 3DP and FLNP swabs were compared in a clinical trial of symptomatic patients at three clinical sites (n=291) using three SARS-CoV-2 EUA tests: a modified version of the CDC Real-time Reverse Transcriptase (RT)-PCR Diagnostic …

Development Of A New Multiplex Real-Time Rt-Pcr Assay For Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) Detection., W. Zhen, G. J. Berry

Journal Articles

This research describes the development of a new multiplex real-time RT-PCR test for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with primers designed to amplify a 108 bp target on the spike surface glycoprotein (S gene) and a hydrolysis TaqMan probe designed to specifically detect SARS-CoV-2. The limit of detection (LOD) and clinical performance of this new assay were evaluated. A LOD study with inactivated virus exhibited performance equal to the modified CDC assay, with a final LOD of 1301 ± 13 genome equivalents/mL for the Northwell Health Laboratories laboratory-developed test (NWHL LDT) versus 1249 ± 14 genome …

Histopathological Evaluation Of Placentas After Diagnosis Of Maternal Sars-Cov-2 Infection., M. Gulersen, L. Prasannan, H. T. Tam, C. N. Metz, B. Rochelson, N. Meirowitz, W. Shan, M. Edelman, K. A. Millington

Journal Articles

Background:The impact of maternal SARS-CoV-2 infection on placental histopathology is not well known. Objectives:To determine if significant placental histopathological changes occur after diagnosis of SARS-CoV-2 infection in pregnancy and whether these changes are correlated with the presence or absence of symptoms associated with infection. Study Design:Retrospective cohort study of women diagnosed with SARS-CoV-2 infection who delivered at a single center from April 9th to April 27th, 2020, and had placental specimens reviewed by pathology. Women with singleton gestations and laboratory-confirmed SARS-CoV-2 infection were eligible for inclusion. Historical controls selected from a cohort of women who delivered 6 months prior to …

Rapid Emergence Of Sars-Cov-2 In The Greater New York Metropolitan Area: Geolocation, Demographics, Positivity Rates, And Hospitalization For 46,793 Persons Tested By Northwell Health., S. B. Reichberg, P. P. Mitra, A. Haghamad, G. Ramrattan, J. M. Crawford, G. J. Berry, K. W. Davidson, A. Drach, S. Duong, S. Juretschko, N. I. Maria, Y. Yang, Y. C. Ziemba, Northwell Covid-19 Research Consortium

Journal Articles

BACKGROUND:In March 2020, the greater New York metropolitan area became an epicenter for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The initial evolution of case incidence has not been well characterized. METHODS:Northwell Health Laboratories tested 46,793 persons for SARS-CoV-2 from March 4 through April 10. The primary outcome measure was a positive reverse-transcription-polymerase-chain-reaction (RT-PCR) test for SARS-CoV-2. The secondary outcomes included patient age, sex, and race if stated; dates the specimen was obtained and the test result; clinical practice site sources; geo-location of patient residence; and hospitalization. RESULTS:From March 8 through April 10, a total of 26,735 of 46,793 …

Histopathologic Evaluation Of Placentas After Diagnosis Of Maternal Severe Acute Respiratory Syndrome Coronavirus 2 Infection., M. Gulersen, L. Prasannan, H. Tam Tam, C. N. Metz, B. Rochelson, N. Meirowitz, W. Shan, M. Edelman, K. A. Millington

Journal Articles

Background: The impact of maternal severe acute respiratory syndrome coronavirus 2 infection on placental histopathology is not well known.

Objective: To determine if any significant placental histopathologic changes occur after the diagnosis of severe acute respiratory syndrome coronavirus 2 infection during pregnancy and whether these changes are correlated with the presence or absence of symptoms associated with the infection.

Study Design: A retrospective cohort study of women diagnosed as having severe acute respiratory syndrome coronavirus 2 infection who delivered at a single center from April 9, 2020 to April 27, 2020, and had placental specimens reviewed by the Department of …

Ass1 Overexpression: A Hallmark Of Sonic Hedgehog Hepatocellular Adenomas; Recommendations For Clinical Practice., M. Sala, D. Gonzales, T. Leste-Lasserre, N. Dugot-Senant, V. Paradis, S. Di Tommaso, J. Dupuy, V. Pitard, C. Fan, F. Saltel, +26 Additional Authors

Journal Articles

Until recently, 10% of hepatocellular adenomas (HCAs) remained unclassified (UHCA). Among the UHCAs, the sonic hedgehog HCA (shHCA) was defined by focal deletions that fuse the promoter of Inhibin beta E chain with GLI1. Prostaglandin D2 synthase was proposed as immunomarker. In parallel, our previous work using proteomic analysis showed that most UHCAs constitute a homogeneous subtype associated with overexpression of argininosuccinate synthase (ASS1). To clarify the use of ASS1 in the HCA classification and avoid misinterpretations of the immunohistochemical staining, the aims of this work were to study (1) the link between shHCA and ASS1 overexpression and (2) the …

The Value Proposition For Pathologists: A Population Health Approach, B. S. Ducatman, A. M. Ducatman, J. M. Crawford, M. Laposata, F. Sanfilippo

Journal Articles

© The Author(s) 2020. The transition to a value-based payment system offers pathologists the opportunity to play an increased role in population health by improving outcomes and safety as well as reducing costs. Although laboratory testing itself accounts for a small portion of health-care spending, laboratory data have significant downstream effects in patient management as well as diagnosis. Pathologists currently are heavily engaged in precision medicine, use of laboratory and pathology test results (including autopsy data) to reduce diagnostic errors, and play leading roles in diagnostic management teams. Additionally, pathologists can use aggregate laboratory data to monitor the health of …

Multiple Myeloma In A Young Female Presenting With Neurological Symptoms., M. Lee, X. Zhang, V. Nguyen, M. Hartley-Brown

Journal Articles

Background:Multiple myeloma is overall the 14th most common malignancy but is rarely seen in those younger than 35 years. Those in the younger age group have been shown to have a more aggressive course but reportedly have had similar responses to treatment compared to older cohorts. Extramedullary plasmacytomas are discrete soft tissue masses of neoplastic monoclonal plasma cells that often occur in patients with multiple myeloma. Case:This case entails a young female with new diagnosis of multiple myeloma and multiple extramedullary plasmacytomas presenting with neurological symptoms. She was treated with CyBorD regimen but was refractory and progressed. Treatment was changed …

Molecular Testing For Acute Respiratory Tract Infections: Clinical And Diagnostic Recommendations From The Idsa's Diagnostics Committee., K. E. Hanson, M. M. Azar, R. Banerjee, A. Chou, R. C. Colgrove, C. C. Ginocchio, M. K. Hayden, M. Holodiny, S. Jain, Diagnostics Committee Of The Infectious Diseases S, +4 Additional Authors

Journal Articles

The clinical signs and symptoms of acute respiratory tract infections (RTIs) are not pathogen specific. Highly sensitive and specific nucleic acid amplification tests have become the diagnostic reference standard for viruses and translation of bacterial assays from basic research to routine clinical practice represents an exciting advance in respiratory medicine. Most recently, molecular diagnostics have played an essential role in the global health response to the novel coronavirus pandemic. How best to use newer molecular tests for RTI in combination with clinical judNorthwell Healthnt and traditional methods can be bewildering given the plethora of available assays and rapidly evolving technologies. …

Reevaluation Of The Us Pathologist Workforce Size, S. J. Robboy, D. Gross, J. Y. Park, E. Kittrie, J. M. Crawford, R. L. Johnson, M. B. Cohen, D. S. Karcher, R. D. Hoffman, W. S. Black-Schaffer, +1 Additional Author

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No abstract provided.

Molecular Pathology Economics 101: An Overview Of Molecular Diagnostics Coding, Coverage, And Reimbursement A Report Of The Association For Molecular Pathology, A. N. Sireci, J. L. Patel, L. Joseph, M. C. Hiemenz, O. C. Rosca, S. K. Caughron, S. A. Thibault-Sennett, T. L. Burke, D. L. Aisner

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No abstract provided.

Evaluation Of Concordance Between Original Death Certifications And An Expert Panel Process In The Determination Of Sudden Unexplained Death In Childhood, L. G. Crandall, J. H. Lee, D. Friedman, K. Lear, K. Maloney, J. K. Pinckard, P. Lin, T. Andrew, A. K. Williamson, O. Devinsky, +8 Additional Authors

Journal Articles

Importance: The true incidence of sudden unexplained death in childhood (SUDC), already the fifth leading category of death among toddlers by current US Centers for Disease Control and Prevention estimates, is potentially veiled by the varied certification processes by medicolegal investigative offices across the United States. Objective: To evaluate the frequency of SUDC incidence, understand its epidemiology, and assess the consistency of death certification among medical examiner and coroner offices in the US death investigation system. Design, Setting, and Participants: In this case series, 2 of 13 forensic pathologists (FPs) conducted masked reviews of 100 cases enrolled in the SUDC …

Performance Evaluation Of The Luminex Aries® C. Difficile Assay In Comparison To Two Other Molecular Assays Within A Multi-Hospital Health Care Center., S. Juretschko, R. Manji, R. Khare, S. Das, S. Dunbar

Journal Articles

Clostridioides difficile infections (CDI) remain a serious issue in the U.S. Both fast and accurate diagnosis of CDI is paramount to achieve immediate infection control initiation, triaging and isolation, as well as appropriate antibiotic treatment. However, both, over- and under-diagnosis can lead to adverse patient outcomes, such as unnecessary administration of antibiotics or unwanted spread of spores in any hospital setting, respectively. In this prospective study, we evaluated the FDA-cleared ARIES® C. difficile Assay and compared its performance and workflow characteristics to the BD MAX Cdiff and Xpert C.difficile/Epi assays. Out of 302 samples tested, 55 (18.2%) samples were positive, …

Systemic P-Anca Vasculitis With Fatal Outcome, Arising In The Setting Of Methimazole Use., S. Hacking, N. N. Uppal, N. Khan, M. Ionescu, V. Bijol

Journal Articles

Here we report a fatal case of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) due to methimazole use in a 64-year-old woman. She was initially hospitalized for abdominal pain and possible colitis, and subsequently developed hematuria, renal failure, and hemoptysis. The serologic work-up revealed positive antinuclear antibody (ANA) and perinuclear-antineutrophilic cytoplasm antibodies (p-ANCA), with positive antimyeloperoxidase. Three weeks following admission, the patient was found to be pulseless, and expired. At autopsy, microscopic review included widespread transmural necrotizing vasculitis and crescentic glomerulonephritis in the kidney, and diffuse pulmonary alveolar hemorrhage; focal coronary artery intimal vasculitis and necrotizing pericarditis were also noted. Several …

Differentiating Noninvasive Follicular Thyroid Neoplasm With Papillary-Like Nuclear Features From Classic Papillary Thyroid Carcinoma: Analysis Of Cytomorphologic Descriptions Using A Novel Machine-Learning Approach., S. Maleki, A. Zandvakili, S. Gera, S. D. Khutti, A. Gersten, S. N. Khader

Journal Articles

Background:Recent studies show various cytomorphologic features that can assist in the differentiation of classic papillary thyroid carcinoma (cPTC) from noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP). Differentiating these two entities changes the clinical management significantly. We evaluated the performance of support vector machine (SVM), a machine learning algorithm, in differentiating cases of NIFTP and encapsulated follicular variant of papillary thyroid carcinoma with no capsular or lymphovascular invasion (EFVPTC) from cases of cPTC with the use of microscopic descriptions. SVM is a supervised learning algorithm used in classification problems. It assigns the input data to one of two categories …

Primary Follicular Lymphoma Of The Bladder Mimicking Inflammatory Condition: Case Report And Review Of The Literature, N. Haghi, X. Zhang, J. Kreshover

Journal Articles

© 2019 The Authors Primary lymphoma of the bladder is rare and may present with nonspecific symptoms and infection. Primary follicular lymphoma of the bladder is extremely rare with only a dozen reported cases. We present one case clinically mimicking an inflammatory process. A 79 year-old woman presents with recurrent urinary tract infections, urinary incontinence and frequency. Cystoscopic examination demonstrated a raised but flat lesion, suspicious for inflammatory lesion. Biopsy revealed a diagnosis of follicular lymphoma, grade 1–2. The presentation of primary follicular lymphoma of the bladder may be nonspecific, therefore it is important to consider this diagnosis in patients …

Comparison Of The Panther Fusion And Bd Max Gbs Assays For Detection Of Group B Streptococcus In Prenatal Screening Specimens., G. J. Berry, F. Zhang, R. Manji, S. Juretschko

Journal Articles

Streptococcus agalactiae, or Group B Streptococcus (GBS) is the cause of early and late-onset GBS disease in neonates and can present as septicemia, meningitis, and pneumonia. Our objective was to compare the performance of two FDA-approved nucleic acid amplification tests (NAATs), the Panther Fusion and BD MAX™ systems, for detection of group B Streptococcus (GBS) in vaginal-rectal screening specimens. A total of 510 vaginal-rectal prepartum specimens were tested simultaneously in both NAATs following broth enrichment. Assay agreement was calculated using the kappa statistics. Overall agreement between assays was 99.0% (505/510; 95% CI: 0.951 to 0.997; kappa = 0.974). Discordant results …

A Comparison Of Daxx (Death Domain Associated Protein 6) In Different Endometrial Carcinoma Histotypes, C. Jin, S. Hacking, M. K. Komforti, M. Nasim

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No abstract provided.

Familial Inheritance Of The 3q29 Microdeletion Syndrome: Case Report And Review, W. A. Khan, N. Cohen, S. A. Scott, E. M. Pereira

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No abstract provided.

The Conscientious Autopsy., A. K. Williamson

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No abstract provided.

Practical Guidance For Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections, C. L. Charlton, E. Babady, C. C. Ginocchio, T. F. Hatchette, R. C. Jerris, Y. Li, M. Loeffelholz, Y. S. Mccarter, M. B. Miller, S. J. Drews, +4 Additional Authors

Journal Articles

No abstract provided.

Cytogenomic Identification And Long-Read Single Molecule Real-Time (Smrt) Sequencing Of A Bardet-Biedl Syndrome 9 (Bbs9) Deletion, J. Reiner, L. Pisani, W. Qiao, R. Singh, Y. Yang, L. Shi, W. A. Khan, R. Sebra, N. Cohen, S. A. Scott, +3 Additional Authors

Journal Articles

No abstract provided.

Overexpression Of Β1 Integrin Contributes To Polarity Reversal And A Poor Prognosis Of Breast Invasive Micropapillary Carcinoma, B. Liu, X. Zheng, F. Meng, Y. Han, Y. Song, F. Liu, S. Li, L. Zhang, X. Zhang, L. Fu, +1 Additional Author

Journal Articles

© Liu et al. Invasive micropapillary carcinoma (IMPC) of the breast is a highly aggressive breast cancer. Polarity reversal exemplified by cluster growth is hypothesized to contribute to the invasiveness and metastasis of IMPC. In this study, we demonstrate that levels of β1 integrin and Rac1 expression were greater in breast IMPC than in invasive breast carcinoma of no specific type and paraneoplastic benign breast tissue. We show that silencing β1 integrin expression using the β1 integrin inhibitor AIIB2 partially restored polarity in IMPC primary cell clusters and downregulated Rac1. Thus, overexpression of β1 integrin upregulates Rac1. Univariate analysis showed …

A Primary Breast Cancer With Distinct Foci Of Estrogen Receptor-Alpha Positive And Negative Cells Derived From The Same Clonal Origin As Revealed By Whole Exome Sequencing, K. Kyker-Snowman, B. Erlanger Avigdor, M. Nasim, A. Cimino-Mathews, S. J. Wheelan, P. Argani, B. H. Park

Journal Articles

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. Background/purpose: Tumor heterogeneity is a now well-recognized phenomenon that can affect the classification, prognosis and treatment of human cancers. Heterogeneity is often described in primary breast cancers based upon histologic subtypes, hormone- and HER2-receptor status, and immunolabeling for various markers, which can be seen within a single tumor as mixed cellular populations, or as separate discrete foci. Experimental design/methods: Here, we present a case report of a patient’s primary breast cancer that had two separate but adjacent histologic components, one that was estrogen receptor (ER) positive, and the other ER …

Stromal Infiltration Of Tumor-Associated Macrophages Conferring Poor Prognosis Of Patients With Basal-Like Breast Carcinoma, M. Yang, Z. Li, M. Ren, S. Li, L. Zhang, X. Zhang, F. Liu

Journal Articles

© Ivyspring International Publisher. Aims: Tumor associated macrophages (TAMs) play a critical role in the initiation and progression of breast cancer. However, their prognostic significance in the molecular subtype of basal-like breast cancer (BLBC) is poorly understood. The aim of this study was to investigate the extent and patterns of TAMs in BLBC and their associations with clinicopathological features and patient survival. Methods and Results: We evaluated TAMs in 200 cases of BLBC by immunohistochemistry using the M2 macrophage marker CD163 and the pan-macrophage marker CD68 in tumor nest and stroma, and assessed their prognostic significance. The study demonstrated that …

Phosphoglycerate Mutase 1 Is Highly Expressed In C6 Glioma Cells And Human Astrocytoma, Z. Liu, J. Ding, C. Du, N. Xu, E. Wang, J. Li, Y. Wang, J. Yu

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No abstract provided.

Automated Real-Time Collection Of Pathogen-Specific Diagnostic Data: Syndromic Infectious Disease Epidemiology, L. Meyers, C. C. Ginocchio, A. N. Faucett, F. S. Nolte, P. H. Gesteland, A. Leber, D. Janowiak, V. Donovan, S. Juretschko, M. A. Poritz, +23 Additional Authors

Journal Articles

© Lindsay Meyers, Christine C Ginocchio, Aimie N Faucett, Frederick S Nolte, Per H Gesteland, Amy Leber, Diane Janowiak,. Background: Health care and public health professionals rely on accurate, real-time monitoring of infectious diseases for outbreak preparedness and response. Early detection of outbreaks is improved by systems that are comprehensive and specific with respect to the pathogen but are rapid in reporting the data. It has proven difficult to implement these requirements on a large scale while maintaining patient privacy. Objective: The aim of this study was to demonstrate the automated export, aggregation, and analysis of infectious disease diagnostic test …

Pleural Effusion In A 7-Year-Old Child - With Unique Cells, N. Gupta, K. Das

Journal Articles

No abstract provided.