Endocrinology, Diabetes, and Metabolism Commons^™

The Role Of The Insulin Receptor Tyrosine Kinase In Two Models Of Insulin Resistance, Nancy Barchus Block

MUSC Theses and Dissertations

Activation of the insulin receptor tyrosine kinase in vitro and in vivo was studied in two rat models of insulin resistance: hypercortisolemia and streptozotocin-induced diabetes. In control rats, intravenous insulin administration resulted in dose-dependent in vivo activation of the skeletal muscle insulin receptor kinase. Chronic cortisone treatment did not affect the kinase activity of receptors solubilized from muscle after activation with insulin in vivo or in vitro. In contrast, diabetes depressed skeletal muscle insulin receptor kinase activation in vivo by ~50%. Diabetes impaired the in vitro insulin stimulation of receptor kinase activity from skeletal muscle and liver towards the substrate …