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- (gRNA) Packaging (1)
- Actin;contractility;microscopy;non-muscle myosin;super-resolution (1)
- Allergic asthma;Immunoglobulin E;Memory B cells (1)
- Antibody response (1)
- CD8+ T cells (1)
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- Dimerization Initiation Sites (1)
- Epstein-Barr Virus (1)
- Footprinting (1)
- Genomic RNA (1)
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- IL-17A signaling (1)
- IL-17RC (1)
- Latent proteins (1)
- Mason-Pfizer Monkey Virus (1)
- Mouse Mammary Tumor Virus (1)
- Mouse mammary tumor virus (MMTV) (1)
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- PI3K/mTOR signaling (1)
- Packaging Signal RNAs Reveal Pal Helix Loops (1)
- Pr77ᴳᵃᵍ (1)
- Primary infection (1)
- Protein expression (1)
- Purine-rich sequence. (1)
- RNA packaging (1)
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- Retrovirus (1)
- Validated Secondary Structures (1)
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Articles 1 - 8 of 8
Full-Text Articles in Medical Microbiology
Elucidating The Mechanism Of Interleukin-17a Signaling In The Pathogenesis Of West Nile Virus, Farzana Nazneen
Elucidating The Mechanism Of Interleukin-17a Signaling In The Pathogenesis Of West Nile Virus, Farzana Nazneen
Dissertations
West Nile Virus (WNV), a neurotropic flavivirus transmitted by mosquitoes, is a leading cause of viral encephalitis in the United States and remains a significant public health concern. Unfortunately, no vaccine or therapeutic intervention is available against WNV infection. Previous studies, including ours, demonstrated that interleukin-17A (IL-17A) signaling increases the cytotoxic potential of CD8+ T cells to facilitate WNV and parasite clearance; however, the molecular mechanism is not understood. IL-17 receptor C (IL-17RC) is an obligatory co-receptor with IL-17 receptor A (IL-17RA) for signaling induced by IL-17A, IL-17A/F, and IL-17F. In this study, we found that IL-17RC deficient ( …
Quantifying Non-Muscle Myosin 2 Filament Dynamics, Melissa Ann Quintanilla
Quantifying Non-Muscle Myosin 2 Filament Dynamics, Melissa Ann Quintanilla
Dissertations
Non-muscle myosin 2 is a motor protein that, when assembled into filaments, works on the actin cytoskeleton to produce contractile forces within cells. This type of myosin dynamically assembles within cells to exert forces necessary for a wide variety of functions including cell division, migration, and multicellular movement. The ability to dynamically assemble contractile networks, and the ability to control the assembly and placement of these myosin filaments in both space and time, is therefore required throughout cell physiology. We used a suite of dynamic, quantitative imaging approaches to identify deterministic factors that drive myosin filament appearance and amplification as …
Lung Resident Memory B Cells Maintain Allergic Ige Responses In The Respiratory Tract, Alexander James Nelson
Lung Resident Memory B Cells Maintain Allergic Ige Responses In The Respiratory Tract, Alexander James Nelson
Dissertations
Immunoglobulin E (IgE) is the primary cause of allergic asthma. Allergen-specific IgE can be detected in the airway of allergic asthma patients even in the absence of circulating IgE, suggesting that IgE can be synthesized locally in the respiratory tract. However, the source of IgE-producing B cells, as well as how IgE responses arise and persist at mucosal sites are not fully understood. To address these questions, we modeled allergic asthma in mice by repeated intranasal administration of ragweed pollen and ovalbumin (OVA). We found that allergen exposure induced the production of local IgE in the airway and formation of …
Exploring The Dynamics Of Primary Epsteinbarr Virus Infection Using A Novel Rabbit, Narendran Reguraman
Exploring The Dynamics Of Primary Epsteinbarr Virus Infection Using A Novel Rabbit, Narendran Reguraman
Dissertations
Epstein-Barr virus (EBV) is a common herpesvirus asymptomatically carried by over 90% of the world population. However, in certain circumstances, the virus can lead to life-threatening diseases. Although the virus was isolated over 55 years ago, several fundamental aspects of the biology of EBV and the mechanism by which this virus induces pathology remain unknown. One major obstacle has been the lack of a suitable small animal model for EBV infection. We have recently shown that healthy rabbits are susceptible to EBV infection, and the virus establishes long-term latent infection typically seen in humans. The main objective of this dissertation …
Insights Into The Packaging Of Mouse Mammary Tumor Virus (Mmtv) Genomic Rna By Identifying Pr77ᴳᴬᴳ Binding Sites Involved During Its Selective Encapsidation, Akhil Chameettachal
Insights Into The Packaging Of Mouse Mammary Tumor Virus (Mmtv) Genomic Rna By Identifying Pr77ᴳᴬᴳ Binding Sites Involved During Its Selective Encapsidation, Akhil Chameettachal
Dissertations
Selective encapsidation and/or packaging of retroviral genomic RNA (gRNA) by Gag during retrovirus assembly is a crucial step for generating infectious virus particles. Despite having been studied extensively, the mechanism by which the retroviral Gag precursor selects and packages the retroviral genome remains largely unclear. Therefore, to understand the molecular mechanism(s) of mouse mammary tumor virus (MMTV) gRNA packaging, as a first step, expression of full-length recombinant Pr77Gag -His6-tag fusion protein in bacteria was done. The recombinant Gag protein was then purified from the soluble fractions of bacterial cultures using immobilized metal affinity chromatography (IMAC) and size exclusion chromatography …
Shape Validated Secondary Structures Of Mason-Pfizer Monkey Virus (Mpmv) And Mouse Mammary Tumor Virus (Mmtv) Packaging Signal Rnas Reveal Pal Helix Loops Functioning As Dimerization Initiation Sites (Dis) Controlling Their Genomic Rna (Grna) Packaging, Suriya Jahan Aktar
Dissertations
The “diploid” genome of retroviruses consists of two strands of RNA that are non-covalently linked as a “dimer” at their 5’ ends. The ubiquitous presence of a dimeric genome among retroviruses suggests that dimerization plays a crucial role in gRNA packaging during viral life-cycle. For almost all retroviruses, determinants of gRNA dimerization and packaging, which are for the most part physically and genetically indistinguishable, reside at the 5’ end of the gRNAs and have been shown to assume higher order structures. Employing a combination of genetic and structural prediction approaches, we have earlier shown that Mason-Pfizer monkey virus (MPMV) and …
The Effect Of Visible Light On Carotenogenesis Of Arthrosporulating Trichophyton Mentagrophytes, Ruth C. Mock
The Effect Of Visible Light On Carotenogenesis Of Arthrosporulating Trichophyton Mentagrophytes, Ruth C. Mock
Dissertations
No abstract provided.
Ultrastructure And Chemical Composition Of Microconidial Walls Of Trichophyton Mentagrophytes, Christine Da-Ruh Wu
Ultrastructure And Chemical Composition Of Microconidial Walls Of Trichophyton Mentagrophytes, Christine Da-Ruh Wu
Dissertations
No abstract provided.