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Articles 121 - 124 of 124
Full-Text Articles in Medical Cell Biology
Aldosterone Stimulates K Secretion Across Mammalian Colon Independent Of Na Absorption, Gerhard Rechkemmer, Dan R. Halm
Aldosterone Stimulates K Secretion Across Mammalian Colon Independent Of Na Absorption, Gerhard Rechkemmer, Dan R. Halm
Neuroscience, Cell Biology & Physiology Faculty Publications
K transport across guinea pig (Cavia porcellus) distal colon was measured in vitro using isotopically determined unidirectional fluxes. Aldosterone stimulated electrogenic Na absorption, as measured by amiloride-sensitive short-circuit current (Isc), and reduced net K absorption from +2.5 ± 0.2 µEq/cm2 per hr to +0.8 ± 0.3 µEq/cm2 per hr (mean ± SEM). Amiloride addition to the mucosal solution did not enhance net K absorption, as expected if inhibiting active Na absorption would reduce active K secretion as in the distal nephron. The amiloride-insensitive Isc was -1.0 ± 0.2 µEq/cm2 per hr (mean ± SEM) …
Electrophysiology Of Flounder Intestinal Mucosa. I. Conductance Properties Ofthe Cellular And Paracellular Pathways, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell
Electrophysiology Of Flounder Intestinal Mucosa. I. Conductance Properties Ofthe Cellular And Paracellular Pathways, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell
Neuroscience, Cell Biology & Physiology Faculty Publications
We evaluated the conductances for ion flow across the cellular and paracellular pathways of flounder intestine using microelectrode techniques and ion-replacement studies. Apical membrane conductance properties are dominated by the presence of Ba-sensitive K channels. An elevated mucosal solution K concentration, [K]m, depolarized the apical membrane potential (ψa) and, at [K]m less than 40 mM, the K dependence of ψa was abolished by 1-2 mM mucosal Ba. The basolateral membrane displayed Cl conductance behavior, as evidenced by depolarization of the basolateral membrane potential (ψb) with reduced serosal Cl concentrations, [Cl]s. …
Electrophysiology Of Flounder Intestinal Mucosa. Ii. Relation Of The Electrical Potential Profile To Coupled Nacl Absorption, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell
Electrophysiology Of Flounder Intestinal Mucosa. Ii. Relation Of The Electrical Potential Profile To Coupled Nacl Absorption, Dan R. Halm, Edward J. Krasny Jr., Raymond A. Frizzell
Neuroscience, Cell Biology & Physiology Faculty Publications
We characterized the hyperpolarization of the electrical potential profile of flounder intestinal cells that accompanies inhibition of NaCl cotransport. Several observations indicate that hyperpolarization of ψa and ψb (Δψa,b) results from inhibition of NaCl entry across the apical membrane: (a) the response was elicited by replacement of mucosal solution Cl or Na by nontransported ions, and (b) mucosal bumetanide or serosal cGMP, inhibitors of NaCl influx, elicited Δψa,b and decreased the transepithelial potential (ψt) in parallel. Regardless of initial values, ψa and ψb approached the equilibrium potential for K (EK …
Metabolism In Preimplantation Mouse Embryos, Harry M. Weitlauf, Gary L. Nieder
Metabolism In Preimplantation Mouse Embryos, Harry M. Weitlauf, Gary L. Nieder
Neuroscience, Cell Biology & Physiology Faculty Publications
The ability of preimplantation mouse embryos to utilize glucose oxidatively is controlled, in part at least, at the level of glycolysis. Various experimental observations are reviewed that indicate the regulatory mechanism in delayed implanting blastocysts involves the classic negative allosteric feedback of high levels of ATP on phosphofructokinase while the situation in 2-cell embryos appears to be more complicated. That is, in addition to the usual negative effect of ATP and citrate on phosphofructokinase, there appears to be a modification of hexokinase that prevents phosphorylation of adequate amounts of glucose and results in low levels of fructose-6-phosphate at the 2-cell …