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Full-Text Articles in Medical Biotechnology
Endocannabinoids And Their Role In Renal Health Of African Americans, William L. Doss Iii, Md, Mba, Abpmr
Endocannabinoids And Their Role In Renal Health Of African Americans, William L. Doss Iii, Md, Mba, Abpmr
JCHP Collaborative Capstone and Research Exchange
Abstract
The current biomarkers used for detecting renal disease Creatinine has long been seen to be inadequate in identifying the extent of renal disease in African and African American populations. CB1 and CB2 receptors are ubiquitous in the human body and their expression is increased with inflammation in the body. In response to the increased expression, the hypothalamus produces endocannabinoids in response. As a result, endocannabinoids can be a biomarker in detecting renal disease where inflammation is a significant factor in renal injury.
Evaluating Bone Fracture Healing Utilizing Doppler Imaging Modes, Shear Wave Elastography, X-Ray And Dual-Energy X-Ray Absorptiometry In A Rabbit Model, Priscilla Machado, Rachel Blackman, Ji-Bin Liu, Flemming Forsberg, Traci Fox
Evaluating Bone Fracture Healing Utilizing Doppler Imaging Modes, Shear Wave Elastography, X-Ray And Dual-Energy X-Ray Absorptiometry In A Rabbit Model, Priscilla Machado, Rachel Blackman, Ji-Bin Liu, Flemming Forsberg, Traci Fox
JCHP Collaborative Capstone and Research Exchange
Abstract
The healing of a bone fracture is a complex, multistage process consisting of inflammation in the local tissue, angiogenesis, callus formation, and eventually, remodeling and restoration of the bone to its original morphology. It is estimated that 5-10% of fractures do not heal properly and exhibit non-union of the fractured bone segments, with long-term complications compared to properly healed fractures. There is great clinical benefit in the ability to have early detection of impending non-union fractures, and diagnostic ultrasound can be used to image formation of the callus in a healing fracture through the use of different imaging modes. …
Localization Of The Multidrug Efflux Pump, Cdr1, In The Fluconazole-Resistant Ent2 Mutant Candida Albicans Strain, Ty Moyer, Sean Chadwick, Scott Gygax, Paula Mccourt
Localization Of The Multidrug Efflux Pump, Cdr1, In The Fluconazole-Resistant Ent2 Mutant Candida Albicans Strain, Ty Moyer, Sean Chadwick, Scott Gygax, Paula Mccourt
JCHP Collaborative Capstone and Research Exchange
Abstract
Deletion of the ENT2 gene in Candida albicans causes the mutant strain to exhibit fluconazole resistance despite having susceptibility prior to this mutation. It was hypothesized that the gain of resistance was sourced by the disruption in endocytic functions, specifically the inability to properly recycle the drug efflux pump, Cdr1. To fully analyze this mutation's effects on the endocytic functions of C. albicans, the Cdr1 protein was tagged in a wild type strain with green fluorescent protein (GFP) and its localization was examined via fluorescence microscopy. To examine Cdr1 protein localization in the ent2 mutant strain, tagging of …
Design And Validation Of A Multiplex Qpcr Assay For The Diagnosis Of Bacterial Vaginosis, Morgan G. Shriver, Richard Sunday, Zi-Xuan Wang, Scott Gygax
Design And Validation Of A Multiplex Qpcr Assay For The Diagnosis Of Bacterial Vaginosis, Morgan G. Shriver, Richard Sunday, Zi-Xuan Wang, Scott Gygax
JCHP Collaborative Capstone and Research Exchange
Abstract
Current diagnostic criteria and treatment options for bacterial vaginosis (BV) commonly result in the misdiagnosis and/or mistreatment of the active infection, as well as subsequent reinfection with BV. This project aims to design and validate a multiplex qPCR assay as a means to accurately detect and quantify populations of dysbiotic Gardnerella vaginalis, Atopobium vaginae, Megasphaera Type 1 & Type 2, Bacterial vaginosis-associated bacterium 2 (BVAB2), as well as the commensal Lactobacillus crispatus, Lactobacillus jensenii, and Lactobacillus gasseri in vaginal specimen for the accurate diagnosis of BV. As these bacterial species are generally unable to be …
Engineered Car19 T-Cells To Demonstrate In Vitro Antileukemia Activity, Khushi Shah, Gian Zhang, Michael Milone
Engineered Car19 T-Cells To Demonstrate In Vitro Antileukemia Activity, Khushi Shah, Gian Zhang, Michael Milone
JCHP Collaborative Capstone and Research Exchange
Abstract
The Chimeric Antigen Receptor T-cells, also known as CAR T-cells, are a revolutionary approach to cancer treatment. They are engineered Tlymphocytes and recombinant receptors for specific antigens. They are mainly responsible for redirecting specificity, activation, and function of T lymphocytes and other immune cells in a single molecule. It attaches to the cancer cells, thereby leading to the destruction of the tumor. The CAR Tcells are made up of different domains, which include Antigen Binding Domain (CD19), Hinge, and Transmembrane Region, followed by Co- Stimulatory Domain (CD28) and T-cell Activation Domain (CD3). The Antigen Binding Domain recognizes the specific …
Capa: Quality Control Testing Of Laboratory Plates, Div Galdi, Bryn Mallon
Capa: Quality Control Testing Of Laboratory Plates, Div Galdi, Bryn Mallon
JCHP Collaborative Capstone and Research Exchange
Abstract
The purpose of a Corrective and Preventive Action (CAPA) investigation is to identify and address errors, deviations, and compliance issues to ensure the integrity, reliability, and quality of research and biomanufacturing. In the biotech industry, where identifying and rectifying root causes of errors is key to ensuring quality data and preventing future errors, CAPA plays a crucial role. The Jefferson Biotechnology (JBT) lab was asked to investigate the sterility of a biotech supplier’s microbiological agar plates, when a client reported contamination in their agar plates, suspecting contamination prior to sleeve opening. By attempting to reproduce our client's results through …
Empowering Students With 5s Organizational Skills For Research Laboratory Optimization, Paula Mccourt, Phd
Empowering Students With 5s Organizational Skills For Research Laboratory Optimization, Paula Mccourt, Phd
JCHP Collaborative Capstone and Research Exchange
Abstract
5S (Sort, Set-in-order, Shine, Standardize and Sustain) is a cost-effective organizational methodology frequently employed by biotech research labs and biomanufacturing to streamline optimization of production. Educational research labs offer experiential learning opportunities and serve as ideal environments for implementing 5S principles within undergraduate and graduate biotechnology curricula. In the Jefferson Biotechnology Program, the newly updated “Lab Design and Stewardship (BT303/503)” lecture and laboratory course, now integrates the 5S methodology into biotech student skill development. Participants in the BT303/503 course are instructed to improve workspaces within the biotechnology laboratory to bolster safety, efficiency, and inventory and equipment management. Through a …
Retention Of Respiratory Therapists: A Systems Thinking Approach, Jerin G. Juby, Dmgt, Rrt
Retention Of Respiratory Therapists: A Systems Thinking Approach, Jerin G. Juby, Dmgt, Rrt
JCHP Collaborative Capstone and Research Exchange
Abstract
Respiratory therapists are an integral part of the healthcare workforce in the United States. The healthcare workforce shortage accelerated by the COVID global pandemic has impacted respiratory therapy departments across the nation, with organizations struggling to hire and retain staff. The prevailing approaches in combating this are linear in nature and do not consider the complexity of the system. This mode of thinking does not adequately consider the characteristics and influences of the containing system, the many elements within the problematic organizational system, or their interdependencies and interactions. This study frames retention as a complex system problem wherein organizations …