Medical Sciences Commons^™

Corticosterone Regulates Both Naturally Occurring And Cocaine‐Induced Dopamine Signaling By Selectively Decreasing Dopamine Uptake, Daniel S. Wheeler, Amanda L. Ebben, Beliz Kurtoglu, Marissa E. Lovell, Austin T. Bohn, Isabella A. Jasek, David A. Baker, John R. Mantsch, Paul J. Gasser, Robert A. Wheeler

Biomedical Sciences Faculty Research and Publications

Stressful and aversive events promote maladaptive reward‐seeking behaviors such as drug addiction by acting, in part, on the mesolimbic dopamine system. Using animal models, data from our laboratory and others show that stress and cocaine can interact to produce a synergistic effect on reward circuitry. This effect is also observed when the stress hormone corticosterone is administered directly into the nucleus accumbens (NAc), indicating that glucocorticoids act locally in dopamine terminal regions to enhance cocaine's effects on dopamine signaling. However, prior studies in behaving animals have not provided mechanistic insight. Using fast‐scan cyclic voltammetry, we examined the effect of systemic …

Retrospective Review Of Biofires Filmarray Gastrointestinal Panels Detection Of Multiple Pathogens In Adults Patients., Patrick Hickey Do, Eric Nellis Md, Anastasia Shnitser Md, Amy Slenker Md

Department of Medicine

No abstract provided.

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Department of Biochemistry: Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation …

Saccade Adaptation Deficits In Developmental Dyslexia Suggest Disruption Of Cerebellar-Dependent Learning, Edward G. Freedman, Sophie Molholm, Michael J. Gray, Daniel Belyusar, John J. Foxe

Publications and Research

Background: Estimates of the prevalence of developmental dyslexia in the general population range from 5% to as many as 10%. Symptoms include reading, writing, and language deficits, but the severity and mix of symptoms can vary widely across individuals. In at least some people with dyslexia, the structure and function of the cerebellum may be disordered. Saccadic adaptation requires proper function of the cerebellum and brainstem circuitry and might provide a simple, noninvasive assay for early identification and sub-phenotyping in populations of children who may have dyslexia.

Methods: Children between the ages of 7 and 15 served as participants in …

Spectrophotometric Analysis Of Thrombolytic Activity: Sata Assay, Masumeh Zamanlu, Morteza Eskandani, Reza Mohammadian, Nazila Entekhabi, Mohammad Rafi, Mehdi Farhoudi

Department of Neurology Faculty Papers

Introduction: Measurement of thrombolytic activity i.e. clot lysis is crucial for research and development of novel thrombolytics. It is also a key factor in assessment of the effectiveness of conventionally used thrombolytic agents in the clinic, which are the choice effective therapies for myocardial infarction and ischemic stroke. Previous methods used for the assessment of thrombolytic activity are often associated with some drawbacks such as being costly, time-consuming, complication and low accuracy. Here, we introduce a simple, economic, relatively accurate and fast method of spectrophotometric analysis of thrombolytic activity (SATA) assay, standardized by tissue plasminogen activator (tPA), which can quantitatively …

Xenobiotic-Induced Activation Of Human Aryl Hydrocarbon Receptor Target Genes In Drosophila Is Mediated By The Epigenetic Chromatin Modifiers, Angelina A. Akishina, J. E. Vorontsova, Roman O. Cherezov, Ilja B. Mertsalov, Olga G. Zatsepina, Mikhail S. Slezinger, Vladislav M. Panin, Svetlana Petruk, Grigori N. Enikolopov, Alexander M. Mazo, Olga B. Simonova, Boris A. Kuzin

Department of Biochemistry and Molecular Biology Faculty Papers

Aryl hydrocarbon receptor (AHR) is the key transcription factor that controls animal development and various adaptive processes. The AHR's target genes are involved in biodegradation of endogenous and exogenous toxins, regulation of immune response, organogenesis, and neurogenesis. Ligand binding is important for the activation of the AHR signaling pathway. Invertebrate AHR homologs are activated by endogenous ligands whereas vertebrate AHR can be activated by both endogenous and exogenous ligands (xenobiotics). Several studies using mammalian cultured cells have demonstrated that transcription of the AHR target genes can be activated by exogenous AHR ligands, but little is known about the effects of …

Effect Of Baseline Serum Calcium On Responses To Extended-Release Calcifediol (Erc) In Stage 3 - 4 Ckd, Nelson Kopyt Do, Fasn, Facp, Stephen A. Strugnell, Akhtar Ashfaq, Martin Petkovich, Charles W. Bishop

Department of Medicine

No abstract provided.

Calpain Inhibition Attenuates Adipose Tissue Inflammation And Fibrosis In Diet-Induced Obese Mice, Latha Muniappan, Aida Javidan, Weihua Jiang, Shayan Mohammadmoradi, Jessica J. Moorleghen, Wendy S. Katz, Anju Balakrishnan, Deborah A. Howatt, Venkateswaran Subramanian

Saha Cardiovascular Research Center Faculty Publications

Adipose tissue macrophages have been proposed as a link between obesity and insulin resistance. However, the mechanisms underlying these processes are not completely defined. Calpains are calcium-dependent neutral cysteine proteases that modulate cellular function and have been implicated in various inflammatory diseases. To define whether activated calpains influence diet-induced obesity and adipose tissue macrophage accumulation, mice that were either wild type (WT) or overexpressing calpastatin (CAST Tg), the endogenous inhibitor of calpains were fed with high (60% kcal) fat diet for 16 weeks. CAST overexpression did not influence high fat diet-induced body weight and fat mass gain throughout the study. …

Six Month Av Fistulae Outcomes Following Local Perianastomotic Delivery Of Sirolimuspreliminary Report From A Us Phase 3 Clinical Trial (Access Trial), Sriram Iyer, Nelson Kopyt Do, Fasn, Facp, Joseph J. Lee, Osman Khawar, Thomas Wooldridge, Nirav Gandhi, Robert I. Lynn, William Paulson, Maria Devita

Department of Medicine

No abstract provided.

Suppression Of Mrnas Encoding Cd63 Family Tetraspanins From The Carcinogenic Liver Fluke Opisthorchis Viverrini Results In Distinct Tegument Phenotypes., Sujittra Chaiyadet, Watchara Krueajampa, Wiphawi Hipkaeo, Yada Plosan, Supawadee Piratae, Javier Sotillo, Michael Smout, Banchob Sripa, Paul J Brindley, Alex Loukas, Thewarach Laha

Microbiology, Immunology, and Tropical Medicine Faculty Publications

The liver fluke Opisthorchis viverrini infects 10 million people in Southeast Asia and causes cholangiocarcinoma (CCA). Fluke secreted and tegumental proteins contribute to the generation of a tumorigenic environment and are targets for drug and vaccine-based control measures. Herein, we identified two tetraspanins belonging to the CD63 family (Ov-TSP-2 and Ov-TSP-3) that are abundantly expressed in the tegument proteome of O. viverrini. Ov-tsp-2 and tsp-3 transcripts were detected in all developmental stages of O. viverrini. Protein fragments corresponding to the large extracellular loop (LEL) of each TSP were produced in recombinant form and antibodies were raised in rabbits. Ov-TSP-2 and …

Week Of October 30, 2017, New York Medical College

InTouch

• School of Medicine Class of 2021 Officially Dons White Coats for the First Time
• NYMC Reaches Out to Puerto Rico
• REDCap Comes to NYMC
• Health Sciences Library Celebrates Faculty Authorship
• Symposium Focuses on Tick-Borne Disease
• NYMC Goes Pink

Bilateral Abducens Palsy Secondary To Metastatic Breast Cancer., Paul Secheresiu Do, Yehia Y. Mishriki Md

Department of Medicine

No abstract provided.

Anti-Yo Positive Paraneoplastic Cerebellar Syndrome In Recurrent Ovarian Carcinoma: A Unique Case To A Rare Phenomenon., Andrew Canakis, Thomas Quinn Do, Ryan Mayo Md

Department of Medicine

No abstract provided.

It's Just Diastolic Heart Failure, Right? A Lesson In Humility From A Patient With Ttr-Amyloid Cardiomyopathy., Nathan Brewster Do, Michael Sumner Do, Ross Biggs Do, Ronald S. Freudenberger Md

Department of Medicine

No abstract provided.

Ppi Induced Hypomagnesemic Hypoparathyroidism Leading To Posterio Reversible Encephalopathy Syndrome., Anam Malik Md, Shannon Davis Do, Yehia Y. Mishriki Md

Department of Medicine

No abstract provided.

A Case Of Refractory Primary Angiitis Of The Central Nervous System., Anam Malik Md, Samer Bolis Do, Susan Kim Md

Department of Medicine

No abstract provided.

Ampa-R Antibody Positive Autoimmune Encephalitis: An Under Recognized Cause Of Acute Mental Status Changes., Joseph Moran Do, George Prousi Md, Stacey Smith Md, Facp

Department of Medicine

No abstract provided.

Encephalopathy Secondary To Aggressive Cd5+ Dlbcl Versus Herpes Zoster Encephalitis., Kathryn Kass Md

Department of Medicine

No abstract provided.

Sick Sinus Syndrome And Chronic Chagas Cardiomyopathy., Sagar Vadhar Do, Benjamin Sanchez Md

Department of Medicine

No abstract provided.

A Seemingly Innocuous Headache Heralding Onset Of Rare Autoimmune Disorder., Thomas Quinn Do, Noor Salam Md

Department of Medicine

No abstract provided.

Novel Immunotherapy Treatments: The Adverse Effects Of Nivolumab., Angela Magdaleno Do, Wael Hanna Md

Department of Medicine

No abstract provided.

Induction Of Peripheral Tolerance In Ongoing Autoimmune Inflammation Requires Interleukin 27 Signaling In Dendritic Cells, Rodolfo Thome, Jason N. Moore, Elisabeth R. Mari, Javad Rasouli

Department of Neurology Faculty Papers

Peripheral tolerance to autoantigens is induced via suppression of self-reactive lymphocytes, stimulation of tolerogenic dendritic cells (DCs) and regulatory T (Treg) cells. Interleukin (IL)-27 induces tolerogenic DCs and Treg cells; however, it is not known whether IL-27 is important for tolerance induction. We immunized wild-type (WT) and IL-27 receptor (WSX-1) knockout mice with MOG_35-55 for induction of experimental autoimmune encephalomyelitis and intravenously (i.v.) injected them with MOG_35-55 after onset of disease to induce i.v. tolerance. i.v. administration of MOG^35-55 reduced disease severity in WT mice, but was ineffective in Wsx^-/- mice. IL-27 signaling in DCs was …

A Coal Miner With Weakness, Fatigue, Nausea, Fever, Chills, Night Sweats And Dyspnea, Larry Nichols

Marshall Journal of Medicine

Delayed or missed diagnoses are a constant hazard in primary care. This is the case report of a 51-year-old coal miner, who presented as an ambulatory outpatient on a Friday with weakness, fatigue, nausea, fever, chills, night sweats and dyspnea. Chest x-ray on Saturday showed pneumonia and pleural effusion, while blood testing showed renal failure, but these results were not known by his physician until Monday, when he was hospitalized with severe sepsis due to fatal Austrian syndrome of pneumococcal pneumonia, endocarditis and meningitis. Analysis of the delay of diagnosis in this case suggests the possibility that a weekend effect …

Gucy2c Maintains Intestinal Lgr5+ Stem Cells By Opposing Er Stress, Crystal Kraft, Jeff A. Rappaport, Adam E. Snook, Amanda M. Pattison, John P. Lynch, Scott A. Waldman

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Long-lived multipotent stem cells (ISCs) at the base of intestinal crypts adjust their phenotypes to accommodate normal maintenance and post-injury regeneration of the epithelium. Their long life, lineage plasticity, and proliferative potential underlie the necessity for tight homeostatic regulation of the ISC compartment. In that context, the guanylate cyclase C (GUCY2C) receptor and its paracrine ligands regulate intestinal epithelial homeostasis, including proliferation, lineage commitment, and DNA damage repair. However, a role for this axis in maintaining ISCs remains unknown. Transgenic mice enabling analysis of ISCs (Lgr5-GFP) in the context of GUCY2C elimination (Gucy2c^-/-) were combined with immunodetection techniques …

The Opioid Crisis., Jennifer Stephens Do

Department of Medicine

No abstract provided.

Inhibition Of Cholesterol Biosynthesis Through Rnf145-Dependent Ubiquitination Of Scap, Li Zhang, Prashant Rajbhandari, Christina Priest, Jaspreet Sandhu, Xiaohui Wu, Ryan E. Temel, Antonio Castrillo, Thomas Q. De Aguiar Vallim, Tamer Sallam, Peter Tontonoz

Saha Cardiovascular Research Center Faculty Publications

Cholesterol homeostasis is maintained through concerted action of the SREBPs and LXRs. Here, we report that RNF145, a previously uncharacterized ER membrane ubiquitin ligase, participates in crosstalk between these critical signaling pathways. RNF145 expression is induced in response to LXR activation and high-cholesterol diet feeding. Transduction of RNF145 into mouse liver inhibits the expression of genes involved in cholesterol biosynthesis and reduces plasma cholesterol levels. Conversely, acute suppression of RNF145 via shRNA-mediated knockdown, or chronic inactivation of RNF145 by genetic deletion, potentiates the expression of cholesterol biosynthetic genes and increases cholesterol levels both in liver and plasma. Mechanistic studies show …

Qi Assessment Of Attending Physician Burnout At Lehigh Valley Health Network (Lvhn) Using The Maslach Burnout Inventory (Mbi)., Pooja Shankar Md, Suzanne J. Templer Do

Department of Medicine

No abstract provided.

Theta-Burst Microstimulation In The Human Entorhinal Area Improves Memory Specificity, Ali S. Titiz, Michael R. H. Hill, Emily A. Mankin, Zahra M. Aghajan, Dawn Eliashiv, Natalia Tchemodanov, Uri Maoz, John Stern, Michelle E. Tran, Peter Schuette, Eric Behnke, Nanthia A. Suthana, Itzhak Fried

Psychology Faculty Articles and Research

The hippocampus is critical for episodic memory, and synaptic changes induced by long-term potentiation (LTP) are thought to underlie memory formation. In rodents, hippocampal LTP may be induced through electrical stimulation of the perforant path. To test whether similar techniques could improve episodic memory in humans, we implemented a microstimulation technique that allowed delivery of low-current electrical stimulation via 100 μm-diameter microelectrodes. As thirteen neurosurgical patients performed a person recognition task, microstimulation was applied in a theta-burst pattern, shown to optimally induce LTP. Microstimulation in the right entorhinal area during learning significantly improved subsequent memory specificity for novel portraits; participants …

Week Of October 23, 2017, New York Medical College

InTouch

• SHSP Hosts Special Dean's Lecture
• NYMC Ranks High in Low Student Loan Default Rates
• Department of Microbiology and Immunology Receives Generous Gift
• 19th Annual Race for Rehab Supports Achilles International
• Students Clubs Enrich Campus Life
• NYMC Has Heart
• D.P.T. Class of 2019 Celebrates National Physical Therapy Month

Epigenetic Impact Of Endocrine Disrupting Chemicals On Lipid Homeostasis And Atherosclerosis: A Pregnane X Receptor-Centric View, Robert N. Helsley, Changcheng Zhou

Pharmacology and Nutritional Sciences Faculty Publications

Despite the major advances in developing diagnostic techniques and effective treatments, atherosclerotic cardiovascular disease (CVD) is still the leading cause of mortality and morbidity worldwide. While considerable progress has been achieved to identify gene variations and environmental factors that contribute to CVD, much less is known about the role of “gene–environment interactions” in predisposing individuals to CVD. Our chemical environment has significantly changed in the last few decades, and there are more than 100,000 synthetic chemicals in the market. Recent large-scale human population studies have associated exposure to certain chemicals including many endocrine disrupting chemicals (EDCs) with increased CVD risk, …