Medical Sciences Commons^™

Microscopy Of Rat Thoracic Aorta In Chronic Ethanol Intake, Feral Öztürk, Ni̇gar Vardi, Nureddi̇n Cengi̇z, Mukaddes Eşrefoğlu, Ali̇ Otlu

Turkish Journal of Medical Sciences

The aim of this study was to determine the histological changes of the thoracic aorta in rats fed on alcohol for a period of 24 weeks. Twenty male Wistar rats were divided into two groups. The ethanol group (n=10) was fed on a modified liquid diet (MLD) containing 7.2% ethanol for 24 weeks. The control group (n=10) was fed on MLD without ethanol throughout the experiment. At the end of the 24 weeks, the carotid arteries of the rats were cannulated and their blood pressures were measured. Ethanol levels measured by using the NAD+/NADH enzyme-spectrophotometric method. Rats were killed by …

Cholesterol Has An Inflammatory Influence In The Effects Of Fats On Metabolism In Endotoxemic Rats, Hali̇t Tanju Besler, Robert Francis Grimble

Turkish Journal of Medical Sciences

We showed that butter suppresses metabolic responses to endotoxin in Wistar rats. Responses included increases in plasma caeruloplasmin and liver zinc and protein concentrations, and decreases in plasma zinc and albumin concentration. However, butter lost its suppressive effect at dietary concentrations above 90g/kg diet. The present study examined whether this phenomenon was due to the total dietary cholesterol intake rather than the intrinsic fatty acid composition of butter. Rats received diets, for four weeks, containing either 100g/kg corn oil, 90 g/kg butter, 190g/kg butter, or 90 g/kg butter supplemented with cholesterol to render the latter two diets identical in cholesterol …

Clinical And Laboratory Correlates Of Frontal Intermittent Rhythmic Delta Activity (Firda), Füsun Korkmaz Alehan, Nathan Watemberg

Turkish Journal of Medical Sciences

This study was carried out to determine whether certain clinical, laboratory, or radiological parameters predispose patients to develop FIRDA in acute situations. Charts of patients in whom FIRDA was detected on an EEG on hospital admission were reviewed. Demographic data, clinical history, physical findings, laboratory results and neuroimaging studies were recorded. Sixty-eight patients with a median age of 56 years were included. Chronic illness was present in 78%. Normal background activity was observed in only 17% of the cases. Epileptiform discharges were uncommon. Abnormal neurological findings were detected in over two thirds of patients. Renal function impairment was present in …

Transcutaneous Measurement Of Bilirubin By Icterometer During Phototherapy On A Bilibed, İpek Akman, Çi̇ğdem Arikan, Hülya Bi̇lgen, Si̇bel Kalaça, Eren Özek

Turkish Journal of Medical Sciences

AIM: We aimed to find the correlation between bilirubin levels measured spectrophotometrically and transcutaneously by an icterometer and investigate if the number of serum bilirubin measurements can be reduced by using an icterometer during phototherapy on a bilibed. METHODS: The study group consisted of 52 babies with total serum bilirubin values less than 18 mg/dl, who were treated by phototherapy at the bilibed in the Neonatology Unit of Marmara University Hospital. Eighty-nine measurements were made from the study population. During phototherapy on the bilibed, the face of the baby was not affected by phototherapy light, allowing transcutaneous bilirubinometry to be …

Pneumopericardium Associated With A Steady Cough, Ahmet Özyazicioğlu, Ahmet Yavuz Balci

Turkish Journal of Medical Sciences

YAZICIOĞLU Ahmet Yavuz BALCI Department of Cardiovascular Surgery, Faculty of Medicine, Atatürk University, Erzurum, Turkey

Analyses Of Surface Dose From High Energy Photon Beams For Different Clinical Setup Parameters, Nadi̇r Küçük, Ayhan Kiliç, Gönül Kemi̇kler, Lütfi̇ Özkan, Kayihan Engi̇n

Turkish Journal of Medical Sciences

Surface and build-up region doses of 6, 15 and 25 MV photon beams were investigated for several clinical setup parameters. Evaluated setups included open fields, physical wedge (PW) fields, virtual wedge (VW) fields and blocked fields. The effects of field size, acrylic block tray, source-to-surface distance (SSD) and off-axis distance on surface dose were determined for each of these setups. Siemens Mevatron MD2 (6-15 MV) and Mevatron-KD2 (6-25 MV) linear accelerators were used to measure both the surface and build-up region doses. The surface dose increased as field size increased in open fields. There was no significant difference between 6 …

Radioimmune Imaging Of Diabetic Foot Infection -Tc-99m-Labelled Antigranulocyte Antibody In Combination With Tc-99m-Methylene Diphosphonate Bone Scintigraphy, Hripsime Artjun Bohchelian, Anelia Dimitrova Klisarova, Lidia Asenova Koeva

Turkish Journal of Medical Sciences

The aim of this study was to evaluate the clinical efficacy of immunoscintigraphy with Tc-99m-labelled antigranulocyte antibody in combination with Tc-99m-methylene-diphosphonate (MDP) bone scanning in diabetic foot infection. The study group comprised 42 Type 2 diabetics with foot infection. Pre- and postprandial blood glucose level, HbA1c, urinary glucose, microalbuminuria, total cholesterol, LDL-cholesterol, HDL-cholesterol, and bacteriological cultures were analyzed. Investigation of transcutaneous oxygen tension, vibration sensation, electromyo-graphy, and Doppler ankle/brachial systolic blood pressure index was carried out. Radiographs centered on the feet were performed. All patients underwent bone scintigraphy with 370 MBq Tc-99m-MDP three hours after injection in 3 projections. Radioimmune …

Prevalence Of Asthma And Asthma-Like And Allergic Symptoms In The Urban Adult Population Of Elazığ, Tuncer Tuğ, Yasemi̇n Açik, Esra Tuğ

Turkish Journal of Medical Sciences

The aim of this study was to determine the prevalence of asthma, asthma-like symptoms and atopy in the adult population of the city of Elazığ. A specific questionnaire, adopted from the European Community Respiratory Health Survey including social status and smoking tendencies of people was filled in by 2454 randomly chosen (1088 male, 1366 female) subjects. The cumulative prevalence of asthma and asthma-like symptoms for males, females and the general population was 22.0%, 28.7%, and 25.7%, respectively. The periodic prevalences of them were 17.3%, 20.5%, and 19.1%, while rates of previously diagnosed asthma were 2.6%, 3.5%, and 3.1%. Subjects suffering …

A Simple Comparison Between Simultaneous Kidney-Pancreas (Skptx) And Kidney-Alone (Ktx) Transplants In Type I Diabetic Nephropaty, Tanju Acar, Arshad Mahmood

Turkish Journal of Medical Sciences

Simultaneous kidney-pancreas transplantation (SKPtx) is an established procedure at many transplant centres for the treatment of end-stage renal disease from type I diabetes mellitus. A successful transplant normalises glucose metabolism and may prevent or reverse some of the complications of diabetes. Concerning graft survival and morbidity rates, the usefulness of this procedure is still questioned by some authors (1,2). We undertook a retrospective study to investigate the differences between survival rates of SKPtx and kidney alone transplant (Ktx) recipients.We also evaluated morbidity rates and complications in both groups. There were no significant differences in 12 and 36 month survival rates. …

Detection Of Human Cytomegalovirus Gene In Formalin-Fixed Paraffin-Embedded Fetal Tissues, Si̇bel Gündeş, Richard Mo, Nomi Konomi, Jaucheng Tzeng, Rhoda Sperling, Xiaoping Li, David Zhang

Turkish Journal of Medical Sciences

NDEŞ^1 Richard MO^1 Nomi KONOMI^1 Jaucheng TZENG^2 Rhoda SPERLING^3 Xiaoping LI^2 David ZHANG^2 Departments of Microbiology^1, Pathology^2, Obstetrics and Gynecology^3, Mount Sinai School of Medicine, New York, New York 10021

In Vitro Esterase Activity Of Carbonic Anhydrase On Total Esterase Activity Level In Serum, Muhammet Fevzi̇ Polat, Barbaros Nalbantoğlu

Turkish Journal of Medical Sciences

The in vitro esterase activity of carbonic anhydrase on total esterase activity level was investigated. This effect was obtained by subtracting the value of net esterase from the value of total esterase in sera with normal (95.22 ± 8.87) and high glucose levels (208.58 ± 87.90). Total esterase activity, net esterase activity and esterase activity of carbonic anhydrase were 20.2 ± 1.9, 18.1 ± 1.8 and 2.1 ± 0.7 U/min/mL serum in sera with a normal glucose level, and 21.3 ± 2.8, 17.9 ± 2.9 and 3.4 ± 1.2 U/min/mL serum in sera with a high glucose level, respectively. Thus, …

The Effect Of Simvastatin Treatment On Plasma Ubiquinone, Blood Atp Concentrations, Total Antioxidant Capacity And Muscle Related Markers, Emel Alteki̇n, Banu Önvural, Canan Çoker, Sema Güneri̇, Sezer Çalişkan

Turkish Journal of Medical Sciences

It has been hypothesized that treating hypercholesterolemic patients with statins will lead not only to a reduction in cholesterol, but also to inhibited synthesis of other compounds that derive from the synthetic pathway of cholesterol. One important by-product is ubiquinone (CQ), which has a pivotal role in mitochondrial electron transport and antioxidant activity. We therefore investigated the effect of 2 months of simvastatin treatment (20 mg/day) on blood ATP concentration, plasma total antioxidant capacity and ubiquinone levels in 17 hypercholesterolemic patients (age range: 40 to 65). To evaluate the possible muscle-related side effects, plasma CK activity and myoglobin concentrations were …

Influence Of Cigarette Smoking On Thyroid Gland Volume: An Ultrasonographic Approach, Fatma Gül Aksoy, Özcan Kesi̇m

Turkish Journal of Medical Sciences

The aim of the present prospective study was to investigate the influence of smoking on ultrasonographically determined thyroid gland volume and echo-texture in a large randomly selected group of 500 healthy subjects of both sexes from a non-iodine deficient area. An accurate ultrasonographic technique and the Brunn method were used to determine the thyroid volume of each volunteer. The examiners had no idea of the smoking habits of the volunteers. In 257 non-smokers the mean value of the right thyroid lobe volume and the left thyroid lobe volume were 7.97 ± 5.27 ml and 6,94 ± 4.82 ml respectively, whereas …

A Case Of Mad Honey Poisoning Presenting With Convulsion: Intoxication Instead Of Alternative Therapy, Embi̇ya Di̇lber, Mukaddes Kalyoncu, Ni̇lgün Yariş, Ayşenur Ökten

Turkish Journal of Medical Sciences

İLBER Mukaddes KALYONCU Nilgün YARIŞ Ayşenur ÖKTEN Department of Pediatrics, Faculty of Medicine, Karadeniz Technical University, Trabzon - Turkey

The Occurrence Of Retinopathy In 11 Eyes After A Solar Eclipse, 1999, Nuray Akyol, Turgut Yilmaz, Burak Turgut

Turkish Journal of Medical Sciences

To present the visual outcome of patients with solar retinopathy and evaluate the effects of treatment on the visual prognosis. One hundred and eighty-eight patients with visual disturbances applied for ophthalmic examination following the solar eclipse on August 11, 1999. All patients underwent routine ophthalmologic examination; those with fundus changes also underwent fundus fluorescein angiography, Amsler grid and computerised perimetry. Among them, nine patients (11 eyes) were evaluated as having solar retinopathy with decreased visual acuity and discernible fundus findings. Mean age was 21.5 (17-34) in this group. Five patients were given treatment, three were on oral methyl prednisolon and …

Enhanced Susceptibility To In Vitro Oxidation Of Apolipoprotein B-Containing Lipoproteins And Antioxidant Status In Patients With Coronary Artery Disease, Zehra Serdar, Emre Sarandöl, Melahat Di̇ri̇can, Akin Serdar, Di̇lek Yeşi̇lbursa, Asuman Tokullugi̇l

Turkish Journal of Medical Sciences

Lipoprotein oxidation may play a pivotal role in the pathogenesis of atherosclerosis, and vitamin E and b-carotene have been proposed to prevent the development and progression of atherosclerosis. The aim of this study was to investigate the oxidative susceptibility of apolipoprotein B (apo B)-containing lipoproteins and their relation with vitamin E and total carotene levels. The susceptibility of apo B-containing lipoproteins to oxidation, plasma malondialdehyde (MDA), serum vitamin E, total carotene, lipid and lipoprotein levels were studied in 177 patients with coronary artery disease (CAD) and 75 non-CAD subjects. All subjects (n = 252) had undergone coronary catheterization. Student's unpaired …

The Relation Of Cerebrospinal Fluid Nitric Oxide Levels To Prognosis And Differential Diagnosis Of Meningitis, Kasim Çeti̇n, Serpi̇l Erol, Hülya Aksoy, Mehmet Akin Taşyaran

Turkish Journal of Medical Sciences

This study was designed to investigate the role of nitric oxide (NO) in the differential diagnosis of bacterial, tuberculous and viral meningitis, and the relation between cerebrospinal fluid (CSF) NO levels and meningitis prognosis. Twenty patients with bacterial meningitis, 9 with tuberculous meningitis, 11 with viral meningitis/meningoencephalitis and 21 control patients were included in the study. CSF NO levels were investigated by measuring the levels of nitrite with a colorimetric test. Mean CSF nitrite levels were 3.9 ± 2.0 µmol/l in bacterial meningitis, 2.7 ± 1.9 µmol/l in tuberculous meningitis, 1.9 ± 1.7 µmol/l in viral meningitis/meningoencephalitis and 1.4 ± …

Individual Cdr3 Regions As Targets For Detection Of Clonal Cells In Acute Lymphocytic Leukemia, Hakan Savli

Turkish Journal of Medical Sciences

VLI University of Helsinki Transplantation Laboratory, Bone Marrow Transplantation Research Team, Helsinki - FINLAND

Oxidative Stress In Mothers And Their Newborns In Different Types Of Labour, İdri̇s Mehmetoğlu Akkuş, Ali̇ Kart, Osman Çağlayan, Meti̇n Çapar, Recep Gökçe

Turkish Journal of Medical Sciences

HMETOĞLU (AKKUŞ)^1 Ali KART^1 Osman ÇAĞLAYAN^1 Metin ÇAPAR^2 Recep GÖKÇE^1 Departments of 1Biochemistry, 2Obstetrics and Gynaecology, Faculty of Medicine, Selçuk University. Konya - Turkey

High-Resolution Physical Map For Chromosome 16q12.1-Q13, The Blau Syndrome Locus, Xiaoju Wang, Helena Kuivaniemi, Gina Bonavita, Charlene J. Williams, Gerard Tromp

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

The Blau syndrome (MIM 186580), an autosomal dominant granulomatous disease, was previously mapped to chromosome 16p12-q21. However, inconsistent physical maps of the region and consequently an unknown order of microsatellite markers, hampered us from further refining the genetic locus for the Blau syndrome. To address this problem, we constructed our own high-resolution physical map for the Blau susceptibility region.

Results

We generated a high-resolution physical map that provides more than 90% coverage of a refined Blau susceptibility region. The map consists of four contigs of sequence tagged site-based bacterial artificial chromosomes with a total of 124 bacterial artificial …

Positive And Negative Roles Of Cd72 In B Cell Function., Hsin-Jung Wu, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Regulation of B cell activation depends on integration of signals transmitted by the B cell receptor (BCR) and a variety of co-receptors. CD72 is a B cell co-receptor that is expressed in all stages of B cell development except plasma cells. Ligation of CD72 enhances B cell growth and differentiation. Recently, the class IV semaphoring, CD100, has been identified as the natural ligand for CD72. Cytoplasmic domain of CD72 has been shown to be associated with SHP-1 leading to the proposal that the positive effects of CD72 on B cell response may result from sequestration of negative signals from BCR. …

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.

Increased Production Of Pro-Inflammatory Cytokines And Enhanced T Cell Responses After Activation Of Human Dendritic Cells With Il-1 And Cd40 Ligand, Amy Wesa, Anne Galy

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Various microbial, inflammatory and immune signals regulate the activation of dendritic cells (DC), determining their ability to interact with naïve T cells and to produce cytokines that direct T cell development. In particular, CD40L and IL-1 cooperatively activate DC to secrete high levels of IL-12. The immuno-stimulatory capacity of such DC is otherwise not well-defined prompting further characterization of the effects of IL-1 and family members on DC activation in comparison with other pro-inflammatory stimuli.

Results

Human DC co-activated in vitro by CD40L and IL-1β expressed numerous cytokine genes including IL-12β, IL-23 p19, IL-1β, IL-1α, IL-1Ra, IL-10, IL-6, …

Search For Intracranial Aneurysm Susceptibility Gene(S) Using Finnish Families, Jane M. Olson, Sompong Vongpunsawad, Helena Kuivaniemi, Antti Ronkainen, Juha Hernesniemi, Markku Ryynã¤Nen, Lee-Lian Kim, Gerard Tromp

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Cerebrovascular disease is the third leading cause of death in the United States, and about one-fourth of cerebrovascular deaths are attributed to ruptured intracranial aneurysms (IA). Epidemiological evidence suggests that IAs cluster in families, and are therefore probably genetic. Identification of individuals at risk for developing IAs by genetic tests will allow concentration of diagnostic imaging on high-risk individuals. We used model-free linkage analysis based on allele sharing with a two-stage design for a genome-wide scan to identify chromosomal regions that may harbor IA loci.

Methods

We previously estimated sibling relative risk in the Finnish population at between …

Ifn-G-Mediated Transcriptional Induction Of The Ido (Indolamine 2,3-Dioxygenase) Gene Requires Activity Of P68/Pkr Protein Kinase, Ni̇lüfer Gülmen İmi̇r, Ayşegül Nasircilar, Hakan Akça, Teki̇nalp Gelen, Mehmet Öğüş, Şeyda Karaveli̇, Osman Ni̇dai̇ Özeş

Turkish Journal of Medical Sciences

We investigated whether the activity of double-stranded RNA activated kinase (p68/PKR) is necessary for induction of IDO (indolamine 2,3 -dioxygenase) gene by IFN-\gamma. For this purpose, we planned to abrogate the function of cellular wt-PKR gene. Clones of ME180 cells expressing the dominant negative form of PKR were selected in the presence of neomycin. Then these cells were treated with IFN-\gamma and accumulation of IDO protein was determined by Western blot analysis. We found that clones expressing the dominant negative form of the PKR gene were not only resistant to IFN-\gamma-mediated killing, but were also incapable of inducing accumulation of …

Intraarticular Cyst Of The Knee, Gülay Yeği̇noğlu, Osman Aynaci, Ahmet Uğur Turhan

Turkish Journal of Medical Sciences

GİNOĞLU^1 Osman AYNACI^2 Ahmet Uğur TURHAN^2 Department of ^1Anatomy, ^2Orthopedic Surgery, School of Medicine, Karadeniz Technical University, 61080 Trabzon - Turkey

Evaluation Of The Prevalence Of Temporomandibular Joint Disorder Syndrome In Dental School Students With Psychometric Analysis, Neci̇p Mutlu, Hasan Herken, Eni̇s Güray, Fati̇h Öz, Abdullah Kalayci

Turkish Journal of Medical Sciences

Aim: Despite the increasing volume of evidence implicating psychological factors in patients with temporomandibular joint (TMJ) disorder syndrome, these aspects of management are frequently neglected. In the diagnosis and treatment of this syndrome, clinical examination must be accompanied by an evaluation of the patients' emotional condition. In our study, the prevalence of TMJ disorders of dental students and the relation between TMJ disorder syndrome and the emotional condition of the patient were evaluated. Methods: The study group consisted of 156 males and 105 females: a total of 261 students. For the psychometric analysis the Minnesota Multiphasic Personality Inventory (MMPI), Symptom …

Comparison Of Antibiotic Resistance Patterns Of Uropathogenic Escherichia Coli In Outpatient And Inpatient Populations From 1990 To 1996 In Bemidji, Mn, Gail R. Conway, Patrick Guilfoile

Journal of the Minnesota Academy of Science

A retrospective study of antibiotic susceptibility was conducted on data from all uropathogenic Escherichia coli submitted to the MeritCare Clinic-Bemidji, MN and the North Country Regional Hospital-Bemidji, MN during 1990, 1993, and 1996. The proportion of antibiotic resistant strains was generally equal or higher in E. coli isolates from community-acquired infections than with E. coli isolates from nosocomial infections. Comparison with antibiotic prescriptions during 1990, 1993, and 1996 provided no clear relation between antibiotics prescribed for outpatient urinary tract infections and the proportion of E. coli strains resistant to those antibiotics. This suggests that factors other than selection for resistance …

Arsenic Trioxide Has Additive Cytotoxic Effects On Mcf-7 Breast Cancer Cell Line With Taxanes, Canfeza Sezgi̇n, Ulus Ali̇ Şanli, Rüçhan Uslu, Erdem Göker

Turkish Journal of Medical Sciences

Breast cancer is the most prevalent cancer in women and an important cause of both morbidity and mortality. Although long-term disease-free survival is offered to women with early stage disease by current treatment modality, some, 30-40% of patients will have metastatic disease. Treatment of patients with metastatic disease is a great challenge for oncologists. Therefore, new drugs and therapeutic options for women with metastatic breast cancer are urgently required. Arsenical compounds have cytotoxic effects on several cancer cell lines including promyelocytic leukemia, esophageal carcinoma, megakaryocytic leukemia, and malignant lymphocytic cell lines. In addition, recent studies showed that arsenic trioxide (As_2O_3) …

Protective Effect Of Nitric Oxide In Lung Injury Associated With Cerulein-Induced Acute Pancreatitis, Abdulkadi̇r Bedi̇rli̇, Erdoğan M. Sözüer, Recep Saraymen, Nai̇l Ersöz, Eser Kiliç

Turkish Journal of Medical Sciences

The aim of this study was to investigate the role of NO in the pathogenesis of lung injury associated with pancreatitis, and the relation between malondialdehyde (MDA) and myeloperoxidase (MPO). Forty Wistar male rats were divided into control or pancreatitis groups, and treated with saline, L-arginine (100 mg/kg i.v.), NO donor, or N-nitro-L-arginine methyl ester (L-NAME) (10 mg/kg i.v.), an inhibitor of NO synthase. Pancreatitis was induced in rats intravenously via the administration of cerulein (10 µg/kg/h) for 4 h). Eight hours after the induction of acute pancreatitis (AP), amylase and lipase in plasma significantly increased in all animals in …