Medical Sciences Commons^™

Conjugated Bilirubin Measurement Re-Examined: A New 'Approach'., Aidan Kelleher

Theses

Conjugated bilirubin is an important but difficult metabolite to measure accurately, elevated levels of conjugated bilirubin in the serum are indicative of obstructive jaundice and therefore can be used to discriminate this disorder from other forms of unconjugated hyper bilirubinaemia.

Aneuploidy Frequencies In Semen Fractions From Ten Oligoasthenoteratozoospermic Patients Donating Sperm For Intracytoplasmic Sperm Injection, Jerome Pfeffer, Myung-Geol Pang, Stanton F. Hoegerman, Christopher J. Osgood, M. W. Stacey, Jacob Mayer, Sergio Oehninger, William G. Kearns

Biological Sciences Faculty Publications

Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.

Design: Prospective study.

Setting: Infertility clinic and molecular genetics laboratory.

Patient(s): Ten patients with severe OAT.

Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.

Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up …

The Effect Of Fish Oil On Liver And Plasma Mda And Antioxidant Status Of Rats, Nuriye Mete, Birgül Işik, Levent Erdi̇nç, Fuat Gürkan

Turkish Journal of Medical Sciences

The question how fish oil (FO) and soybean oil (SO) change the state of antioxidant and the lipid peroxidation in liver and plasma of rats and the protective role of vitamin E were investigated. Six-week-old forty male Sprague-Dawley rats were divided into five groups. One of the groups fed a control diet, another one fed SO, while the other three groups fed FO diet at the level of 10% Vitamin E supplementation was maintained to three different levels 3, 50, 150 IU/per kg diet in the FO diets and 3 IU vitamin E per kg of the SO diet. At …

Quantification Of Total Collagen In Rabbit Tendonby The Sirius Red Method, Dilek Taşkiran, Emin Taşkiran, Hüseyin Yercan, Fatma Z. Kutay

Turkish Journal of Medical Sciences

No abstract provided.

Effect Of Psychological Stress On Nasal Immunity, Erdal Seren, Abdülcemal Ümit Işik, Mehmet İmamoglu, Osman Bahadir, Mehmet Arif Köroğlu, Yavuz Tekeli̇oğlu

Turkish Journal of Medical Sciences

Stress activates the hypotalamic-pituitary- adrenal axis, increases circulating glucocorticoids, and is associated with alterations in immune functions. Nasal mucosa is richly innervated by sympathetic nerve fiber and nasal patency changes in response to various sympathetic stimuli. These fibres form close neuroeffector junctions with lymphocytes and macrophages. Nasal lavage and peripheral blood samples were collected from healthy medical students one hour before their exams and a week later, after the declaration of results of the exams were announced. We determined and compared the distribution of T lymphocyte subsets in students under psychological stress The T cell: natural killer cell, T helper …

Genitourinary Abnormalities In Children With Urinary Tract Infections, Ayşe Balat, L. Leighton Hill

Turkish Journal of Medical Sciences

Urinary-tract infections (UTI) are common infectious diseases in childhood. The presence of UTI may be an indicator of a serious genitourinary (GU) abnormality that requires medical intervention. We retrospectively reviewed the charts of children discharged with a diagnosis of UTI from the Texas Children's Hospital, Houston, Texas, between July 1, 1991 and June 30, 1994. The purpose of the study was to determine how many patients had underlying GU abnormalities, the specific type of abnormality, and the microorganism causing the UTI. During the 3-year period, there were 48,382 discharges from the hospital, and 1,221 (2.5%) of those discharges were patients …

Effect Of Exercise On Blood Antioxidant Status And Erythrocyte Lipid Peroxidation: Role Of Dietary Supplementation Of Vitamin E, Mustafa Kelle, Hüda Di̇ken, Abdurrahman Şermet, Mukadder Atmaca, Cemi̇l Tümer

Turkish Journal of Medical Sciences

We tested the effects of moderate physical exercise on the blood antioxidant capacity and erythrocyte lipid peroxidation in 21 Wistar albino rats. Erythrocyte superoxide dismutase (SOD) activity increased significantly (p

Administration Of Granulocyte Colonystimulating Factor (G-Csf) In Experimentallyinfected Rats, Ömer Cevi̇t, Ruhi̇ye Cevi̇t, Gökhan Köylüoğlu, Di̇lara İçağasioğlu, Asim Gülteki̇n, Fatoş Tanzer

Turkish Journal of Medical Sciences

We investigated the effect of antibiotic or G-CSF plus antibiotic therapy given to E. coli septic rats. In our study, 80 rats were inoculated transperitoneally with 1 ml of a solution containing 10 7 colony forming units per milliliter of E. coli. Rats were divided into four treatment groups before being inoculaded with E. coli in a random fashion. Therapy was then started with cefotaxime (150 mg/kg intraperitonelally) and either Granulocyte-Colony Stimulation Factor (G-CSF; 30 µg/kg intarperitoneally) or placebo 24 hours after inoculation of E. coli for 3 consective days. Group A 1 , sepsis-antibiotics group, had a mortality rate …

A Novel Clinical And Laboratory Based Approach To Coronary Heart Disease: Relationship Between Angiography Findings, Antioxidant Enzymes And No, Bülent Sözmen, Fevzi̇ye Uysal, Dervi̇ş Gazi̇tepe, Leyla Aslan, Eser Yildirim Sözmen

Turkish Journal of Medical Sciences

Atherosclerosis is the most common manifestation of cardiovascular disease. However the exact mechanisms that take place in its pathogenesis have not yet been completely understood. Free radicals and lipid peroxidation may play an important role in the pathogenesis of atherosclerosis and endothelial dysfunction also may be an aggravating factor in this process. Unfortunately all of the previous studies have focused on the atherosclerotic tissue. Our aim was to establish a relationship between the antioxidant enzymes and plasma nitrate as an index of whole-body, endogenous nitric oxide production in coronary heart disease and also the relationship between these parameters and the …

Urinary Serotonin And 5-Hydroxyindolacetic Acid Levels In Preeclampsia, Pervi̇n Vural, Cemi̇l Akgül, Mukaddes Canbaz, Alkan Yildirim

Turkish Journal of Medical Sciences

The role of serotonin in the pathogenesis of hypertension which is one of the main symptoms of preeclampsia is of growing importance. The levels of serotonin (5-Hydroxytryptamin or 5-HT) and its metabolite, 5-Hydroxyindolacetic acid (5-HIAA) in twenty-four hour urine samples taken from severe preeclamptic and normotensive pregnant women were determined by colon chromotography and spectrophotometry. In preeclamptic pregnant women there was a significant increase in 5-HT levels (P

Cytogenetic And Phenotypic Findings In Turkish Patients With Fanconi's Anemia, Hati̇ce Ilgin, Ayşe Nurten Akarsu, Fatma Işik Bökesoy

Turkish Journal of Medical Sciences

Fanconi's anemia is an autosomal recessive disorder with manifestation of chromosomal instability inducible by alkylating agents. Since 1989 we studied 85 patients with suspected Fanconi's anemia (FA) to confirm diagnosis. Cytogenetic analyses were performed on spontaneous and induced cultures of peripheral blood samples. Induction was carried with mitomycin-C (MMC) or diepoxybutane (DEB). The normal range of spontaneous, MMC and DEB-induced breaks were 0.00-0.10, 0.00-0.16 and 0.00-0.16 per cell respectively. Fanconi's anemia was confirmed when breaks were above the normal threshold. According to this criterion 59 patients were confirmed as having FA. Some of the siblings were also investigated to exclude …

The Effect Of Race On The Exposure Of Theinternal Auditory Canal Via The Retrosigmoidapproach In Turkish Subjects, Eni̇s Alpi̇n Güneri̇, Handan Çakmakçi, Ahmet Ömer İki̇z, Keri̇m Ceryan

Turkish Journal of Medical Sciences

Hearing conservation in acoustic neuroma surgery via the retrosigmoid approach depends upon a good exposure of the internal auditory canal from behind avoiding labyrinthine injury. The accessibility is influenced largely by the individual characteristics of the temporal bone and the variability of the surgical landmarks is the result of different skull base shapes among different races. The aim of this study was to evaluate the effect of race on the exposure of the internal auditory canal of Turkish subjects via the retrosigmoid approach. Axial temporal bone CT scans of 40 Turkish individuals were studied by measuring the relevant parameters and …

A Patient With Three Simultaneously Occurring Malignancies, Bahar Kiliçarslan, Gülay Özbi̇li̇m, Gökbay Elpek, Şeyda Karaveli̇, Mi̇ne Üner

Turkish Journal of Medical Sciences

No abstract provided.

Time Dependent Changes In The Cristaneuroepithelium Resulting From Gentamicinototoxicity In The Chinchilla, Hasan Tanyeri̇, Ivan Lopez, Larry Hoffman

Turkish Journal of Medical Sciences

This experiment was conducted to investigate the time-dependent effects of gentamicin (GM) on the neuroepithelial morphology of the posterior crista ampullares (PCA) of the adult chinchilla. The time course of GM-induced HC pathology was documented through measurements of HC density as well as through HC morphologic evaluation. HC morphology was examined under light microscopy and HC density measurements were documented as a function of the time course of GM-induced ototoxicity. Six groups of animals (n=12) were treated with daily injections of 30 mg/kg of GM subcutaneously (SC) for six days and were sacrificed 1, 2, 3, 4, 6 and 8 …

The Evaluation Of Microanastomoses In Rats Withcolor Doppler Ultrasonography, Bahri̇ Üstünsöz, İbrahi̇m Somuncu, Mahmut Kömürcü, Mustafa Başşbozkurt, Sabri̇ Ateşalp, Alper Alemdaroğlu

Turkish Journal of Medical Sciences

Purpose: To evaluate the vascular patency of microanastomoses in rats with color Doppler ultrasonography. Materials and Methods: 15 superficial femoral and 15 carotid arterial end-to-end anastomoses were performed in 30 white rats. All arteries were evaluated pre- and post-operatively by color Doppler ultrasonography with a 7.5 mHz linear transducer (Acuson 128HP). All results were correlated with surgery after final Doppler evaluation. Results: The subjects were divided into three groups: (a) patent anastomosis; (b) partial occlusive anastomosis; (c) total occlusive anastomosis. The predictive value ratios for the patency group was 100% (15/15), that for the partial occlusive group was 67% (6/9) …

The In Vitro Effect Of Plasmodium Bergheiantigen Lysate On Natural Killer (Nk) Cellcytotoxic Activity, Cemaletti̇n Albayi̇, Ayşegül Yücel, Kemal Çeber, Turgut İmi̇r

Turkish Journal of Medical Sciences

Plasmodium berghei is the rodent equivalent of Plasmodium falciparum which causes cerebral malaria in humans. However, P. berghei does not cause cerebral malaria in BALB/c mice, instead it leads to parasitemia, fever and some degree of liver damage. Although different aspects of immunological response have been intensively investigated in malaria; this the first time the in vitro effect of P. berghei antigen lysate on murine NK cytotoxic activity was studied. It was observed that this parasite antigen lysate suppresed the cytolytic activity of NK cells in a dose-dependent manner. Because the suppression of NK cell activity in a dose-dependent manner …

Rate Of Carriage, Serotype Distribution And Penicilin Resistance Of Streptococcus Pneumoniae In Healty Children, Burçi̇n Şener, Sevtap Arikan, M. Alper Ergi̇n, Ayfer Günalp

Turkish Journal of Medical Sciences

The purpose of this study was to define carriage rates for Streptococcus pneumoniae in a given population in Ankara and also to determine the sertypes and penicilin resistance of these strains. Oropharyngeal swabs were taken from a total of 661 children between 1 and 11 years of age living in a province of Ankara between January 1995 and January 1997. Serotyping was performed by detection of the Quellung reaction. Penicilin susceptibilities of the isolates were screened by agar dilution method according to the guidlines of the National Committe for Clinical Laboratory Standards (NCCLS). The total rate of pneumococcal carriage in …

Generalized Bcg Lymphadenitis In An Infant:Diagnostic Dilemma With Lepromatous Leprosyand Gaucher Disease On Fine Needle Aspiration, Mustafa Hacimustafaoğlu, Mehmet Okan, Ömer Yerci̇, Suna Gedi̇koğlu, İrfan Kiriştioğlu, İbrahi̇m Ildirim

Turkish Journal of Medical Sciences

A two-year old male infant with generalized BCG lymphadenitis is presented. There was no obvious immunodeficiency disorder detected in the patient. BCG vaccine had been given at two months of age, and the lymphadenopathy developed at 21 months of age. Fine needle aspiration biopsy of the lymph node on Giemsa stain revealed macrophages very similar to Gaucher cells. These cells also resembled macrophages seen in lepromatous leprosy. They were engorged with acid fast bacilli and had streaked or wrinkled cytoplasm. Mycobacterium bovis was cultured from the lymph nodes and the patients improved with antituberculous therapy.

Opinions Of Assistant Doctors In Süleymandemirel University Medical Faculty Aboutstatistical Knowledge, Mustafa Öztürk, Veli̇ddi̇n Selçuk Engi̇n, Ahmet Nesi̇mi̇ Ki̇şi̇oğlu

Turkish Journal of Medical Sciences

In scientific investigations; statistical knowledge is essential for collection and analysis of data properly as well as correct interpretation of literature information. In this study that was made in March 1997, opinions of 46 assistant doctors in Süleyman Demirel University Medicine Faculty about statistical knowledge were investigated by a questionnaire. All respondents have agreed on the fact that comprehension of the statistical process in an article was necessary for throughout understanding. Besides, 43 of them (93.5%) admitted that their present statistical knowledge was few or absent at all. All assistant doctors stated that a statistical education program need to be …

Chromosomal Aberrations In Peripherallymphocytes Of A High-Voltage Power Linemanexposed To Electromagnetic Fields, M. Emi̇n Erdal, Nurten Erdal, Si̇bel Oğuzkan

Turkish Journal of Medical Sciences

No abstract provided.

Infantile Galactosialidosis Associated Withvitamin D Deficiency Rickets, Koray Boduroğlu, Ergül Tunçbi̇lek, Turgay Coşkun, Canan Uçar

Turkish Journal of Medical Sciences

Galactosialidosis is an autosomal recessive disease with combined deficiency of two lysosomal enzymes due to the lack of a protective protein. We report on a boy with infantile galactosialidosis who has an intermediate phenotype and vitamin D deficiency rickets. Due to the possible role of vitamin D deficiency in the pathogenesis of dysostosis multiplex we recommend that patients with lysosomal storage disease should be supplemented with vitamin D.

Nadph-Diaphorase-Reactive Neurons In Rat Basal Ganglia, Gülgün Kayalioğlu, Fi̇gen Gövsa, Mete Ertürk

Turkish Journal of Medical Sciences

NADPH-d reaction was performed on nine male Sprague-Dawley rats and morphometry of NADPH-d(+) cells was investigated in caudate-putamen, globus pallidus and ventral pallidum. In caudate-putamen we observed intensely stained NADPH-d (+) cells. Maximum diameter and minimum diameters of the NADPH-d (+) cells in caudate-putamen was measured as 19.19±0.04 \mum and 10.42±0.12 \mum, consequently. In globus pallidus, moderately stained NADPH-d (+) cells were observed. We measured the maximum and minimum diameters of the NADPH-d (+) cells as 19.93±0.24 \mum and 10.42±0.12 \mum, consequently. In ventral pallidum, medium-sized, moderately stained cells were present. Maximum and minimum diameters of the NADPH-d (+) cells …

Protein C, Protein S And Antithrombin Iii Levelsin A Rabbit Sepsis Model, Mehmet Totan, Cengi̇z Di̇lber, Davut Albayrak

Turkish Journal of Medical Sciences

The consumption of coagulation inhibitors will augment coagulation triggered excessively in sepsis. The determination of levels of these inhibitors may open new substitution treatment schedules to overcome the plateau of the achieved improvement ratio with antimicrobial treatment. Staphylococcus aureus ATCC 25923, 1x10 9 per kg day for two days was administered intravenously to 30 albino rabbits to induce sepsis. Clinical and hematological signs were determined and sepsis was determined with blood culture. The complete blood count, protein C, protein S and antithrombin III levels were examined at the baseline and after sepsis development. The mean proportions of baseline to sepsis …

Immunohistochemical Study On Thepcna-Immunoreactivity In The Uterus Of Ratsovariectomized Or Treated With Antiestrogenclomiphene Citrate, Leyla Canpolat, İbrahi̇m Hani̇fi̇ Özercan, Nurten Bozlak, Cengi̇z Doğan

Turkish Journal of Medical Sciences

PCNA (proliferating cell nuclear antigen) immunoreactivity in the uterus of mature rats was investigated after long term administration of clomiphene citrate and ovariectomy. In the luminal epithelium and glandular epithelium and endometrial stroma regions, few PCNA-immunoreactive cells were observed. PCNA reactivity was estimated for the luminal and glandular epithelium and for the stromal cells. Treatment of clomiphene citrate decreased the proliferation of the luminal epithelial cell and stromal cells to the same extent as ovariectomy. In both of these groups, the proportion of anti-PCNA positive cells in the glandular epithelium was significantly higher than in the luminal epihelium and stromal …

The Effects Of Insulin On Serum Levels Of Apo A-I Containing Lipoprotein Particles In Syndrome X Patients With Coronary Heart Disease, Meti̇n Yildirimkaya, Cumhur Kilinç, Mustafa Özkan, Kurtuluş Yilmaz, Yavuz Nari̇n

Turkish Journal of Medical Sciences

Syndrome X consists of low serum high density lipoprotein-cholesterol (HDL-C), raised serum triglyceride, glucose intolerance, increased blood pressure, abdominal obesity and insulin resistance which is associated with increased risk of coronary heart disease (CHD). Apolipoprotein (apo) A-I containing lipoprotein particles, lipoprotein A-I (Lp A-I) and lipoprotein A-I:A-II (Lp A-I:A-II.) may have different metabolic functions. The purpose of this study was to evaluate the relationship between insulin and apo A-I containing lipoproteins in syndrome X patients with or without CHD. We selected 38 male patients with syndrome X and divided into two groups: The one with CHD (n=21) and the other …

Determination Of Viable Myocardium In Patientswith Ischaemic Left Ventricular Dysfunction:Comparison Of Thallium-201 Single-Photonemission Computed Tomography With Nitrateand Dobutamine Echocardiography, Mustafa Cemri̇, Ridvan Yalçin, Bülent Boyaci, Özlem Kapucu, Tamer Atasever, Ati̇ye Çengel, Övsev Dörtlemez, Hali̇s Dörtlemez

Turkish Journal of Medical Sciences

The method of Thallium - 201- single - photon emission computed tomography (Tl-201 SPECT) have been commonly used to detect uncontractile but viable myocardium in patients with ischemic left ventricular dysfunction. The aim of this study was to establish the value of nitrate echocardiography (NE) and low dose dobutamine echocardiography (LDDE) to detect viable myocardium when compared to Tl-201 SPECT. Twently patients (16 M, 4F; mean age: 56.4±9.6 years) who had anterior myocardial infarction in the last three months were studied. Within the same week, but on different days NE, LDDE and Tl-201 SPECT procedures were performed to the patients. …

Neutrophil Chemotaxis In Acutely Infected Andclinically Stable Cystic Fibrosis Patients, Burçi̇n Şener, Gülşen Hasçeli̇k, Uğur Özçeli̇k, Ayfer Günalp, Ayhan Göçmen

Turkish Journal of Medical Sciences

Brochopulmonary infection in cystic fibrosis (CF) patients is associated with chronic progressive lung disease. The role of host factors, such as neutrophil functions, in the progressive pathologic processes in the respiratory tract of these patients, is poorly defined. Neutrophil chemotaxis is one of the first mechanisms of host defense to become activated after bacterial invasion has occurred. This study was aimed to evaluate the role of neutrophil chemotaxis in CF and also to determine whether an acute bacterial infection and the nutritional status can affect neutrophil chemotaxis or not. Twelve acutely infected and 12 clinically stable CF patients and 10 …

Psychosomatic Symptoms In Patients With Alopecia Areata And Vitiligo, İbrahi̇m Kökçam, Nevzat Akyar, Yunus Saral, Nalan K. Oğuzhanoğlu

Turkish Journal of Medical Sciences

Vitiligo and alopecia areata present an excellent opportunity to f ocus on the effect of impaired appearance on the lives of the persons with these diseases. Emotional stress has precipitated vitiligo and alopecia areata in some patients. Twenty-eight patients suffering from alopecia areata and vitiligo and twenty healthy persons at ages between ten and sixty underwent psychological tests of examination with special regard to verification of their psychiatric symptoms. The results were compared for all groups. The results revealed that depressive symptomatology in patients with vitiligo and alopecia areata was higher than in the control group (respectively, 55%, 36%, 15%). …

Characterization Of Catalytic Phenotype Of \Beta-Galactosidasefrom Laci Mutant, E. Coli Csh-36,As A Tool For The Management Of Lactoseintolerance, Ayşegül Ayyildiz

Turkish Journal of Medical Sciences

Objectives: Lactose intolerance is a common public health problem with 5-100% prevalance among different populations of the world. Use of \beta-galactosidase to reduce the lactose content in food or consumption of special products of milk or exogeneous lactase enzyme are among the approaches taken to manage lactose intolerance. Recombinant \beta -galactosidase as well as \beta -galactosidase derived from different sources have variances in their bioefficiency. Thermodynamic properties of \beta -galactosidase determines the industrial efficiency. In this \beta -galactosidase from E. coli CSH-36, a constitutive expressor of enzyme was investigated for enzyme biofunctionality. Methods: The enzyme was purified by cell lysis, …

Dose Dependent Changes In The Cristaneuroepitheium Resulting From Gentamicinototoxicity In The Chinchilla, Hasan Tanyeri̇, Ivan Lopez, Larry Hoffman

Turkish Journal of Medical Sciences

This experiment was conducted to investigate the dose-dependent effects of gentamicin (GM) on the neuroepithelial morphology of the posterior crista ampullares (PCA) of the adult chinchilla. The experiment focused on hair cell (HC) morphology to monitor GM's dose-dependent effects and also to define an ototoxically-efficient and safe dose what would subsequently be used in a time course study. Chinchillas (n=13) were treated with subcutaneous (SC) daily doses of 120 mg/kg, 60 mg/kg, and 30 mg/kg GM, respectively, for six days. Light microscopy observations were made from the excised PCA of two animals from each group that survided the complete treatment …