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Digestive System Diseases Commons

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Full-Text Articles in Digestive System Diseases

Mechanism Of Toxt-Dependent Transcriptional Activation At The Vibrio Cholerae Tcpa Promoter, Robin R. Hulbert, Ronald K. Taylor Oct 2002

Mechanism Of Toxt-Dependent Transcriptional Activation At The Vibrio Cholerae Tcpa Promoter, Robin R. Hulbert, Ronald K. Taylor

Dartmouth Scholarship

The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the mechanism of ToxT-dependent transcriptional activation at tcpA. Deletion analysis showed that a region from −95 to +2 was sufficient for ToxT binding and activation, both of which were simultaneously lost when the deletion was extended to −63. A collection of point mutations generated by error-prone PCR revealed two small regions required …


Health And Development: Infectious Intestinal Diseases In A Transborder Region (United States-Mexico), Flavia Bianchi Jan 2002

Health And Development: Infectious Intestinal Diseases In A Transborder Region (United States-Mexico), Flavia Bianchi

Theses and Dissertations (Comprehensive)

Intestinal infectious diseases are considered the northern Mexican border’s most obvious challenge facing children. The Mexico-US Border region is a 200-kilometer border zone that extends 100 kilometers on either side of an imaginary borderline and stretches 3,141 kilometers from the Pacific Ocean to the Gulf of Mexico. In 2000, the Pan American Health Organization (PAHO), the World Health Organization’s representative for the Americas, published a study of the top causes of mortality between 1995 and 1997 to estimate the absolute burden of mortality in the primary Mexican and American border community, collectively known as the Sister Cities. Using the PAHO …