Oral Biology and Oral Pathology Commons^™

Effects Of Jagged1 On Mirna Expression In Dental Stem Cells, Promphakkon Kulthanaamondhita

Chulalongkorn University Theses and Dissertations (Chula ETD)

Notch signaling is activated in dental pulp tissues after direct pulp capping treatment in vivo, implying a role in tertiary dentin formation. Furthermore, Notch signaling also participates in periodontal ligament development and maturation. However, its mechanism of action remains to be elucidated. The present study investigated the expression profile of miRNAs in Notch-activated human dental stem pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs), examined their potential cellular targets and validated the functions of miRNAs in modulating the odonto / osteogenic properties. DPSCs and PDLSCs were treated with indirect immobilized Jagged1. The miRNA expression profile was examined using …

Probing The Porphyromonas Gingivalis Nitrosative Stress Response Network, Alexandra Galina

Theses and Dissertations

Objective: To persist in the oral cavity, the perio-pathogen Porphyromonas gingivalis must have an efficient nitrosative stress response mechanism. To better understand how P. gingivalis responds to nitrite (NO₂^-) we must determine what genes are involved in the nitrosative stress response network.

Methods: Through genomic screening, a list of genes was generated that were hypothesized to be part of the nitrosative stress response network and believed to be involved in nitrate/nitrite metabolism or redox state of the cells. Protein coding regions were replaced with the ermF sequence by allelic exchange mutagenesis. 15 mutants were created …

Effects Of ⍺-Lactalbumin Hydrolysate On Human Oral Squamous Cell Carcinoma Cell Lines, Agustina Putri Kintan Arini

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background : Alpha-lactalbumin, the protein from human and bovine milk has been found to be promising as an alternative of anticancer agent Objective: To investigate the effects of lactalbumin enzymatic hydrolysate (LAH) on cell proliferation, migration, and mRNA expression of MMP on human squamous cell carcinoma (hSCC) cell lines, in vitro. Materials and Methods: Tongue (HSC-4 and 7) and pharyngeal (HN-30 and 31) hSCC cell lines were treated with a two-fold dilution of LAH (0.39-100 mg/ml). Cell viability and cell proliferation were examined by MTT assay. Cell migration was investigated by a scratch wound healing assay. Gene expression of metastasis-associated …

Comorbidity Of Hypertension And Chronic Renal Failure And Their Impact On Oral Complications: A Cross-Sectional Study., Dalia Ghalwash, Sara Abdelwahed, Asmaa Abobakr

Dentistry

The present study aimed to investigate the influence of both end-stage renal diseases (ESRD) and hypertension (HTN) on the various oral manifestations and periodontal condition, to evaluate and compare the oral manifestations and periodontal health status in hypertensive patients with ESRD versus control, and to explore any possible correlation of the oral findings with systemic disease status. This was a cross-sectional study which included two equal groups of patients, Group I were hypertensive patients with ESRD and Group II were hypertensive patients only. All patients were hypertensive and were on antihypertensive medications. Regarding the incidence of oral lesions in both …

Enhancing Dental Aligners With Direct 3d Printing Manufacturing, Erin Clark, Lauren Ickes, Tyler Madison

Williams Honors College, Honors Research Projects

Replacement of thermoforming with direct 3d printed aligners, molding appliances for dental and alveolar movement. Currently the process involved requires 3d printing models and plastic thermoforming, trimming and polishing to fabricate the end product. If possible a product that could be 3d printed that had stress retention, crack resistance, and stain resistance properties delivered in thickness between .030 and .040 inches.

Rehabilitation Of A Patient With Single Complete Tooth-Supported Overdenture Against The Unmodified Natural Dentition Using Functionally Generated Path Technique: A Case Report, Akanksha Shinde, Nilesh Bulbule, Varun Bhatt, Gayathri Balachandran, Amit Jagtap

International Arab Journal of Dentistry

Meyer’s proposed chew-in technique also called functionally generated path (FGP) technique, is indicated for the restoration of an edentulous arch that is opposed by natural teeth as it does seem to provide the most accurate method of recording occlusal patterns, also help to minimize the effects of negative factors, and to overcome the deficiencies of articulators. As stated by Rudd and Morrow, this technique is especially suitable for tooth-supported complete dentures, due to inherent stability of tooth-supported record bases helps in recording jaw relationships accurately. Whenever a single complete denture is opposed by natural dentition, problems observed are abrasion of …

Effect Of Simvastatin On Stem Cell Isolated From Apical Papilla (Scap) In Vitro, Paak Rewthamrongsris

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background and Objective: Simvastatin, known for lipid-lowering, shows promise in modulating stem cell behavior. This research investigates simvastatin's impact on apical papilla stem cells (SCAPs) in vitro. Methods: SCAPs were isolated, characterized, and exposed to varying simvastatin doses. Viability and proliferation were assessed with LIVE/DEAD and MTT assays. Cell cycle and apoptosis were examined via flow cytometry, and migration capacity was evaluated through scratch tests. Alizarin Red S staining and qPCR were used for odonto/osteogenic differentiation analysis. Results: Simvastatin reduced SCAP proliferation on days 3 and 7 and decreased colony size and density. Enhanced apoptosis and increased G0 subpopulation were …

Knowledge And Attitude Towards Oral Health Related Behaviors Of International Students In Chulalongkorn University, During The Pandemic Of Covid-19, Isi Susanti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: To determine the association of knowledge and attitude toward oral healthcare behavior of overseas university students staying in Thailand between January 2020 and July 2022 and explore the experiences of their oral health problems. Methods: A cross-sectional study conducted using an online survey in English operated through the Google platform by convenience sampling among overseas Chulalongkorn University students. The sample size was calculated by the Yamane formula with a minimum sample size of 297 after adding 10% compensation. Descriptive statistics, Chisquare test, t-test, ANOVA, and Pearson correlations were employed using IBM SPSS version 29. Results: Of 311 overseas students, …

Autogenous Raw Tooth Particles Versus Xenograft For Alveolar Ridge Preservation: A Prospective Controlled Clinical Trial, Basel Mahardawi

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: The use of extracted teeth has been recently introduced as an option for bone grafting. However, the current method requires special machines and solutions, which poses significant time and cost. The aim of this study was to evaluate the clinical performance of autogenous Raw Tooth Particles (RTP), a grafting material made from a ground tooth using basic equipment, for alveolar ridge preservation. Materials and methods: 14 and 13 sites (23 patients) were included for the study and control group, respectively. The control group received Bio-Oss® xenograft. Radiographic measurements (in millimeters) were taken at the baseline and at the 4-month …

Studying Gene Function In Streptococcus Sanguinis Using Rna-Sequencing Analysis, Ahmed Ismail

Theses and Dissertations

The oral cavity hosts hundreds of bacterial species, amongst them are the Streptococci family. More specifically, S. sanguinis is a benign bacteria in the oral cavity to protect the tooth surface from S. mutans. which is a pathogen causing dental caries. While S. sanguinis is harmless in the oral cavity, it becomes pathogenic once entering the bloodstream and causing Infective Endocarditis (IE). To further study S. sanguinis and eventually develop an effective drug to treat IE caused by S. sanguinis, a gene expression study was conducted. In order to study gene expression, RNA-sequencing was conducted, and appropriate methods to remove …

Evaluating Repeated Dental Emergency Visits Related To Third Molar Pathologies In An Urgent Dental Care Center, Armando U. Rivera Dds, Linda Rasubala Dds, Phd, Gilbert H. Schulenberg Dds, Changyong Feng Phd, Hans Malmstrom Dds, Yan-Fang Ren Dds, Mph, Phd

The New York State Dental Journal

Individuals with symptomatic third molar pathologies frequently seek dental care at emergency departments or urgent dental care centers. This is becoming a dental public health problem, when repeated dental emergency appointments are sought for treatment of symptomatic contralateral wisdom teeth. Body: We designed a retrospective review study of adults 18 years and older with an initial dental emergency visit related to third molar pathology at a single dental urgent care center, at the University of Rochester. Conclusion: A sub-optimal oral health is a predictor for future emergency dental visits, due to pathology of remaining third molars.

Diagnosis And Management Of Nasopalatine Duct Cyst: A Report Of Two Cases And Literature Review, Feiyi Sun Dds, Harry Dym Dds

The New York State Dental Journal

The nasopalatine duct cyst (NPDC) is one of the most common non-odontogenic, non-neoplastic developmental cysts. It arises from the epithelial remnants of the nasopalatine duct within the incisive canal and crosses the midline of the anterior maxilla. Most cases are asymptomatic and either are discovered by chance on radiographs or present as a soft tissue swelling, pain, or drainage in the palate. The present study describes the diagnosis and surgical management of two NPDC cases at the Department of Oral and Maxillofacial Surgery at the Brooklyn Hospital Center.