Dentistry Commons^™

Carbosilane Dendrimers: Drug And Gene Delivery Applications, Navid Rabiee, Shokooh Ahmadvand, Sepideh Ahmadi, Yousef Fatahi, Rassoul Dinarvand, Mojtaba Bagherzadeh, Mohammad Rabiee, Mohammadreza Tahriri, Lobat Tayebi, Michael R. Hamblin

School of Dentistry Faculty Research and Publications

Carbosilane dendrimers are a particular type of dendrimer structure that has been used as delivery vehicles for drugs and nucleic acids. They have a defined structure according to their generation number, and their terminal groups can be rendered cationic or anionic. The cationic charges can address the limitation of electrostatic repulsion between the negatively charged phosphate groups of nucleic acids and negatively charged cell membranes. Specific drugs can be loaded into the central part of the dendrimer or attached at the exterior, and the overall positive charge may improve the efficacy of anti-inflammatory drugs. One promising feature of dendrimers is …

Three-Dimensional In Vitro Oral Mucosa Models Of Fungal And Bacterial Infections, Fahimeh Tabatabaei, Keyvan Moharamzadeh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Oral mucosa is the target tissue for many microorganisms involved in periodontitis and other infectious diseases affecting the oral cavity. Three-dimensional (3D) in vitro and ex vivo oral mucosa equivalents have been used for oral disease modeling and investigation of the mechanisms of oral bacterial and fungal infections. This review was conducted to analyze different studies using 3D oral mucosa models for the evaluation of the interactions of different microorganisms with oral mucosa. In this study, based on our inclusion criteria, 43 articles were selected and analyzed. Different types of 3D oral mucosa models of bacterial and fungal infections were …

Bonding Crowns And Bridges With Resin Cement, Nathaniel C. Lawson, Sharukh Khajotia, Ana K. Bedran-Russo, Kevin Frazier, Jacob Park, Ariene A. Leme-Kraus, Olivia Urquhart

School of Dentistry Faculty Research and Publications

Background

Bonding crowns and bridges with resin cement can improve retention and reinforcement of the restoration. However, there is variation in the steps taken by different practitioners to achieve this goal.

Methods

The authors developed a survey on bonding dental crowns and bridges with resin cement and distributed it electronically to the American Dental Association Clinical Evaluators (ACE) Panel on May 22, 2020. The survey remained open for 2 weeks. Descriptive data analysis was conducted using SAS Version 9.4.

Results

A total of 326 panelists responded to the survey, and 86% of respondents who place crowns or bridges use resin …

Fibroblast Encapsulation In Gelatin Methacryloyl (Gelma) Versus Collagen Hydrogel As Substrates For Oral Mucosa Tissue Engineering, Fahimeh Tabatabaei, Keyvan Moharamzadeh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Purpose

Over the past decades, a variety of biomaterials have been investigated in terms of their suitability for oral mucosa tissue engineering. The aim of this study was to compare collagen and GelMA hydrogels as connective tissue scaffolds for fibroblasts and as substrates for seeding and culture of oral epithelial keratinocyte cells.

Methods

Human primary oral fibroblast and keratinocyte cells were isolated from gingival biopsies. The mixture of fibroblasts with GelMA or collagen gel were aliquoted within six-well tissue culture plate inserts and cross-linked using visible light or reconstitution buffer/heat, respectively. The viability of fibroblasts in the hydrogels was investigated …

Aptamer-Conjugated Plga Nanoparticles For Delivery And Imaging Of Cancer Therapeutic Drugs, Maryam Hashemi, Azarmidokht Shamshiri, Majid Saeedi, Lobat Tayebi, Rezvan Yazdian-Robati

School of Dentistry Faculty Research and Publications

Most problems associated with chemotherapeutic agents involve non-specific cytotoxicity, low intratumoral accumulation and drug resistance. Targeted drug delivery systems (TDDS) based on nanoparticles (NPs) are a new strategy for better therapeutic efficiency, along with reduction of side effects commonly seen with cancer drugs. Poly (lactic-co-glycolic acid) (PLGA), as one of the furthest developed synthetic polymer, has gained significant attention because of excellent properties—including biodegradability and biocompatibility, controlled release of drug, protection of drug or gene from decomposition and ability to modify surface with targeting agents for both cancer diagnosis and therapy. Aptamers are single-stranded RNA or DNA that can fold …

Shedding Light On The Role Of Keratinocyte-Derived Extracellular Vesicles On Skin-Homing Cells, Golara Nasiri, Negar Azarpira, Aliakbar Alizadeh, Sanaz Goshtasbi, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Extracellular vesicles (EVs) are secretory lipid membranes with the ability to regulate cellular functions by exchanging biological components between different cells. Resident skin cells such as keratinocytes, fibroblasts, melanocytes, and inflammatory cells can secrete different types of EVs depending on their biological state. These vesicles can influence the physiological properties and pathological processes of skin, such as pigmentation, cutaneous immunity, and wound healing. Since keratinocytes constitute the majority of skin cells, secreted EVs from these cells may alter the pathophysiological behavior of other skin cells. This paper reviews the contents of keratinocyte-derived EVs and their impact on fibroblasts, melanocytes, and …

A Review On The Biodistribution, Pharmacokinetics And Toxicity Of Bismuth-Based Nanomaterials, Samireh Badrigilan, Fatemeh Heydarpanahi, Jalal Choupani, Mehdi Jaymand, Hadi Samadian, Mojtaba Hoseini-Ghahfarokhi, Thomas J. Webster, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Here, bismuth-based nanomaterials (Bi-based NMs) are introduced as promising theranostic agents to enhance image contrast as well as for the therapeutic gain for numerous diseases. However, understanding the interaction of such novel developed nanoparticles (NPs) within a biological environment is a requisite for the translation of any promising agent from the lab bench to the clinic. This interaction delineates the fate of NPs after circulation in the body. In an ideal setting, a nano-based therapeutic agent should be eliminated via the renal clearance pathway, meanwhile it should have specific targeting to a diseased organ to reach an effective dose and …

Evaluation Of Mechanical And Biocompatibility Properties Of Hydroxyapatite/Manganese Dioxide Nanocomposite Scaffolds For Bone Tissue Engineering Application, Fatemeh Azizi, Fatemeh Heidari, Farahnaz Fahimipour, Mohammad Sajjadnejad, Daryoosh Vashaee, Lobat Tayebi

School of Dentistry Faculty Research and Publications

The aim of this research was to evaluate the mechanical properties, biocompatibility, and degradation behavior of scaffolds made of pure hydroxyapatite (HA) and HA‐modified by MnO₂ for bone tissue engineering applications. HA and MnO₂ were developed using sol‐gel and precipitation methods, respectively. The scaffolds properties were characterized using X‐ray diffraction (XRD), Fourier transform spectroscopy (FTIR), scanning electron microcopy (SEM), energy dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). The interaction of scaffold with cells was assessed using in vitro cell proliferation and alkaline phosphatase (ALP) assays. The obtained results indicate that the HA/ MnO₂ scaffolds possess higher …

Magnetic Dual-Template Molecularly Imprinted Polymer Based On Syringe-To-Syringe Magnetic Solid-Phase Microextraction For Selective Enrichment Of P-Coumaric Acid And Ferulic Acid From Pomegranate, Grape, And Orange Samples, Ebrahim Alipanahpour Dil, Mehrorang Ghaedi, Arash Asfaram, Fatemah Mehrabi, Ardeshir Shokrollahi, Amir Abbas Matin, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Magnetic dual-template molecularly imprinted polymer (Fe₃O₄@SiO₂-MDMIP) was prepared to enrich and determine both p-Coumaric acid (p-CA) and ferulic acid (FA) based on syringe-to-syringe magnetic solid-phase microextraction (SS-MSPME). The obtained MDMIP was characterized and recognized, and then its adsorbing performance was studied. Based on the results, the Fe₃O₄@SiO₂-MDMIP indicated selective recognition towards p-CA and FA with large adsorption capacity. The optimization of MDMIP–SS-MSPME conditions (pH, Fe₃O₄@SiO₂-MDMIP mass, NaCl concentration, number of cycle, and elution volume) were conducted using the central composite design (CCD). …

Biomineralization, Strength And Cytocompatibility Improvement Of Bredigite Scaffolds Through Doping/Coating, R. Keihan, A. R. Ghorbani, E. Salahinejad, E. Sharifi, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Coprecipitation-derived, sacrificial polymeric (urethane) foam-fabricated bredigite (Ca₇MgSi₄O₁₆) scaffolds were processed by individual and combined treatments of fluoride doping and poly (lactic-co-glycolic acid) (PLGA) coating and then studied in terms of structure, mechanical strength, bioactivity and cell biocompatibility in vitro. According to scanning electron microscopy and Archimedes porosimetry, the geometrical characteristics of pores for all the scaffolds are in the appropriate range for hard tissue regeneration applications. The apatite-formation ability of the samples immersed in a simulated body fluid is improved by doping for both the bare and coated conditions, based on …

Effect Of Dentin Biomodification Delivered By Experimental Acidic And Neutral Primers On Resin Adhesion, Lívia Tosi Trevelin, Yvette Alania, Mathew Mathew, Rasika S. Phansalkar, Shao-Nong Chen, Guido F. Pauli, Ana K. Bedran-Russo

School of Dentistry Faculty Research and Publications

Objectives

Proanthocyanidins (PACs) are biocompounds mimicking native collagen cross-links. The effective and practical delivery of any biocompound is pivotal for clinical usage. The aim was to investigate the dentin biomodification and effective formation of dentin–resin biointerfaces of two highly bioactive PAC-rich extracts, Vitis vinifera (Vv) and Camellia sinensis (Cs), delivered using neutral (NP) or acidic (AP) rinse-out primer approaches.

Methods

The depth of dentin demineralization (optical profilometry), dentin biomodification (apparent modulus of elasticity, collagen auto-fluorescence) and properties of dentin–resin interfaces (microtensile bond strength - μTBS, and micro-permeability) were investigated. NP consisted of either 15% Vv or Cs applied for …

Effect Of Fabrication Method On The Structure And Properties Of A Nanostructured Nickel-Free Stainless Steel, L. Heidari, A. Tangestani, M. J. Hadianfard, Daryoosh Vashaee, Lobat Tayebi

School of Dentistry Faculty Research and Publications

An ASTM F2581 nanostructured stainless steel was fabricated by two different powder metallurgy routes; Hot Powder Forging (HPF) and Binder Assisted Extrusion (BAE) methods. Their structure and mechanical properties were investigated and compared. In both fabrication methods, the alloy powder was made by using main alloying elements through mechanical alloying, along with the addition of a sintering aid. In the BAE method, a paste was prepared by mixing alloy powders with polymer followed by cold extrusion, polymer removal, and sintering. In the HPF method, the alloy powders were hot forged under high pressure. The structure and the size of the …

Left Ventricular Global And Regional Deformation In Arrhythmic Myxomatous Bileaflet Mitral Valve Prolapse Syndrome, Lakshmi Muthukumar, Arshad Jahangir, M. Faud Jan, Patrycja Galazka, Matt Umland, Mckenzie R. Schweitzer, Ana Cristina Perez Moreno, Maharaj Singh, Bijoy K. Khandheria, A. Jamil Tajik

School of Dentistry Faculty Research and Publications

No abstract provided.

Synthesis Of Novel Reducing Agent For Formation Of Metronidazole-Capped Silver Nanoparticle And Evaluating Antibacterial Efficiency In Gram-Positive And Gram-Negative Bacteria, Fatemeh Farjadian, Amin Reza Akbarizadeh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

In this study, a new type of silver nanoparticles capped with metronidazolium based ionic liquid is synthesized. By this aim, metronidazole is altered to ionic-liquid type structure with citrate counter ion as reducing agent. The produced reducing agent was characterized using ¹HNMR and ¹³CNMR and FT-IR. The capability of metronidazolium-based reducing agent in formation and capping silver nanoparticles was examined in a chemical reaction. More specifically, synthesized silver nanoparticles were synthesized and capped with metronidazolium-citrate based ionic liquid, while the formation of particles in 48 h was monitored by UV-Vis spectroscopy. Fourier transform infrared spectroscopy showed the presence …

Adoptive Treg Cell-Based Immunotherapy: Frontier Therapeutic Aspects In Rheumatoid Arthritis, Mahdi Zavvar, Sara Assadiasl, Sina Zargaran, Maryam Akhtari, Behzad Poopak, Rassoul Dinarvand, Yousef Fatahi, Lobat Tayebi, Narjes Soleimanifar, Mohammad Hossein Nicknam

School of Dentistry Faculty Research and Publications

The major current focus on treating rheumatoid arthritis is to put an end to long-term treatments and instead, specifically block widespread immunosuppression by developing antigen-specific tolerance, while also permitting an intact immune response toward other antigens to occur. There have been promising preclinical findings regarding adoptive Treg cells immunotherapy with a critically responsible function in the prevention of autoimmunity, tissue repair and regeneration, which make them an attractive candidate to develop effective therapeutic approaches to achieve this interesting concept in many human immune-mediated diseases, such as rheumatoid arthritis. Ex vivo or invivo manipulation protocols are not only utilized to correct …

Rare A-Type, Spiro-Type, And Highly Oligomeric Proanthocyanidins From Pinus Massoniana, Bin Zhou, Yvette Alania, Mariana C. Reis, James Mcalpine, Ana K. Bedran-Russo, Guido F. Pauli, Shao-Nong Chen

School of Dentistry Faculty Research and Publications

An investigation of the dental bioactive proanthocyanidin (PAC) oligomer fractions led to three structurally distinct new PACs (1–3) from pine bark. Pinutwindoublin (1) is the first reported trimer with double A-type interflavanyl linkages (2α→O→5,4α→6 and 2α→O→7,4α→8). Pinuspirotetrin (2) represents the first reported PAC tetramer with a heterodimeric framework consisting of one spiro-type and one A-type dimer. Pinumassohexin (3) was elucidated as a mixed A + B-type hexamer that consists of a peanut-derived tetramer, peanut procyanidin E, and an A-type dimer (5). Compound 3 increased the modulus of elasticity of dentin …

Tri- And Tetrameric Proanthocyanidins With Dentin Bioactivities From Pinus Massoniana, Bin Zhou, Yvette Alania, Mariana C. Reis, Rasika S. Phansalkar, Joo-Won Nam, James Mcalpine, Shao-Nong Chen, Ana K. Bedran-Russo, Guido F. Pauli

School of Dentistry Faculty Research and Publications

Guided by dentin biomechanical bioactivity, this phytochemical study led to the elucidation of an extended set of structurally demanding proanthocyanidins (PACs). Unambiguous structure determination involved detailed spectroscopic and chemical characterization of four A-type dimers (2 and 4–6), seven trimers (10–16), and six tetramers (17–22). New outcomes confirm the feasibility of determining the absolute configuration of the catechol monomers in oligomeric PACs by one-dimensional (1D) and two-dimensional (2D) NMR. Electronic circular dichroism as well as phloroglucinolysis followed by mass spectrometry and chiral phase high-performance liquid chromatography (HPLC) analysis generated the necessary chiral reference data. In …

Synthesis And Characterization Of 3d-Printed Functionally Graded Porous Titanium Alloy, Ahmed Hindy, Farzam Farahmand, Fereydoun Pourdanesh, Maryam Torshabi, A. Hadi Al Janabi, Morteza Rasoulianboroujeni, Lobat Tayebi, Fahimeh Tabatabaei

School of Dentistry Faculty Research and Publications

This study aims to 3D print titanium alloy constructs incorporating gradient of porosities, from the fully dense core to the porous outer surface. Gradient porous specimens were prepared using selective laser melting (SLM). Fully dense specimens fabricated by SLM were used as the control group. Characterization of samples was done using X-ray tomography, uniaxial compression testing, and optical and scanning electron microscopes. The biocompatibility of fabricated samples was investigated using human periodontal ligament stem cells via assessment of cell attachment, viability, and proliferation by direct and indirect assays. The data were analyzed using ANOVA and Tukey’s post hoc test. Characterization …

Electrical And Mechanical Properties Of Bzt − Xbct Lead‐Free Piezoceramics, Raziye Hayati, Mohammad Fayazi, Hadi Diargar, Mohammad Kaveh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

In this study, lead‐free (1 − x)Ba(Zr_0.2Ti_0.8)O3 − x(Ba_0.7Ca_0.3)TiO₃ compositions are synthesized via conventional solid oxide route, and the ceramics are fabricated with normal sintering in air. The effects of composition fluctuations on dielectric, piezoelectric, and mechanical properties are investigated. The phase structure and the microstructure are analyzed with X‐ray diffraction and scanning electron microscopy. The best dielectric and piezoelectric properties of ε_r = 11 207 and d₃₃ = 330 pC/N were obtained for BZT−0.35BCT and BZT−0.5BCT ceramics, respectively. The mechanical behavior—in terms of Vickers hardness and compressive and …

The Adverse Effects Of Radiotherapy On The Structure Of Dental Hard Tissues And Longevity Of Dental Restoration, Miguel Angel Munoz, Carolina Garin-Correa, Wilfredo Gonzalez-Arriagada, Ximena Quintela Davila, Patricio Haberle, Ana K. Bedran-Russo, Issis Luque-Martinez

School of Dentistry Faculty Research and Publications

Purpose:

The main goal of this study was to evaluate the impact of different ionizing radiation doses on the mineral (carbonate/phosphate ratio, crystallinity index [CI]) and organic (amide III/phosphate, amide I sub-band ratios) structures, as well as the microhardness, of enamel and dentin, along with their influence on the bonding strength stability of the etch-and-rinse (ER) and self-etch (SE) dental adhesive strategies.

Materials and methods:

Enamel and dentin human tissue specimens were irradiated (with 0, 20, 40, and 70 Gy radiation doses, respectively) and sectioned to perform an attenuated total reflection-Fourier transform IR spectroscopy assay (ATR-FTIR) and the Vickers microhardness …

Recent Advances In Designing 5-Fluorouracil Delivery Systems: A Stepping Stone In The Safe Treatment Of Colorectal Cancer, Elaheh Entezar-Almahdi, Soliman Mohammadi-Samani, Lobat Tayebi, Fatemeh Farjadian

School of Dentistry Faculty Research and Publications

5-Fluorouracil (5-FU) has become one of the most widely employed antimetabolite chemotherapeutic agents in recent decades. It is considered a first line antineoplastic agent for the treatment of colorectal cancer. Unfortunately, chemotherapy with 5-FU has several limitations, including its short half-life, high cytotoxicity and low bioavailability. In order to overcome the drawbacks of 5-FU and enhance its therapeutic efficiency, many scientific groups have focused on designing a new delivery system to successfully deliver 5-FU to tumor sites. We provide a comprehensive review on different strategies to design effective delivery systems, including nanoformulations, drug-conjugate formulations and other strategies for the delivery …

Microfluidic Fabrication Of Microcarriers With Sequential Delivery Of Vegf And Bmp-2 For Bone Regeneration, Erfan Dashtimoghadam, Farahnaz Fahimipour, Nikita Tongas, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Wound instability and poor functional vascularization in bone tissue engineering lead to lack of tissue integration and ultimate failure of engineered grafts. In order to harness the regenerative potential of growth factors and stimulate bone healing, present study aims to design multifunctional cell therapy microcarriers with the capability of sequential delivery of essential growth factors, bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF). An on-chip double emulsion method was implemented to generate monodisperse VEGF encapsulated microcarriers. Bio-inspired poly(3,4-dihydroxyphenethylamine) (PDA) was then functionalized to the microcarriers surface for BMP-2 conjugation. The microcarriers were seeded with mesenchymal stem cells …

Dental Light-Curing Units: An American Dental Association Clinical Evaluators Panel Survey, Kevin Frazier, Ana K. Bedran-Russo, Nathaniel C. Lawson, Jacob Park, Sharukh Khajotia, Olivia Urquhart

School of Dentistry Faculty Research and Publications

Background

The ability to polymerize light-activated dental materials with dental light-curing units (DLCUs) has revolutionized dentistry. However, proper DLCU use is essential for ensuring the effectiveness and performance of these materials.

Methods

The authors developed an electronic cross-sectional survey in the American Dental Association Qualtrics Research Core platform. The survey included questions about DLCU use, unit type and selection, training, maintenance, technique, and safety measures. The authors deployed the survey to 809 American Dental Association Clinical Evaluators (ACE) panelists on October 9, 2019, and sent reminder links to nonrespondents 1 week later. They conducted exploratory and descriptive analyses using SAS …

Burgeoning Polymer Nano Blends For Improved Controlled Drug Release: A Review, Saeid Maghsoudi, Bahareh Taghavi Shahraki, Navid Rabiee, Yousef Fatahi, Rassoul Dinarvand, Maryam Tavakolizadeh, Sepideh Ahmadi, Mohammad Rabiee, Mojtaba Bagherzadeh, Ali Pourjavadi, Hassan Farhadnejad, Mohammadreza Tahriri, Thomas J. Webster, Lobat Tayebi

School of Dentistry Faculty Research and Publications

With continual rapid developments in the biomedical field and understanding of the important mechanisms and pharmacokinetics of biological molecules, controlled drug delivery systems (CDDSs) have been at the forefront over conventional drug delivery systems. Over the past several years, scientists have placed boundless energy and time into exploiting a wide variety of excipients, particularly diverse polymers, both natural and synthetic. More recently, the development of nano polymer blends has achieved noteworthy attention due to their amazing properties, such as biocompatibility, biodegradability and more importantly, their pivotal role in controlled and sustained drug release in vitro and in vivo. These compounds …

Aptamer Hybrid Nanocomplexes As Targeting Components For Antibiotic/Gene Delivery Systems And Diagnostics: A Review, Navid Rabiee, Sepideh Ahmadi, Zeynab Arab, Mojtaba Bagherzadeh, Moein Safarkhani, Behzad Nasseri, Mohammad Rabiee, Mohammadreza Tahriri, Thomas J. Webster, Lobat Tayebi

School of Dentistry Faculty Research and Publications

With the passage of time and more advanced societies, there is a greater emergence and incidence of disease and necessity for improved treatments. In this respect, nowadays, aptamers, with their better efficiency at diagnosing and treating diseases than antibodies, are at the center of attention. Here, in this review, we first investigate aptamer function in various fields (such as the detection and remedy of pathogens, modification of nanoparticles, antibiotic delivery and gene delivery). Then, we present aptamer-conjugated nanocomplexes as the main and efficient factor in gene delivery. Finally, we focus on the targeted co-delivery of genes and drugs by nanocomplexes, …

Clinical Case-Based Problem-Solving Examination In Periodontics, Swati Y. Rawal, Arndt Guentsch

School of Dentistry Faculty Research and Publications

No abstract provided.

Does The Method Of Visualization Impact The Performance Of A New Surgical Task In Novice Subjects?, Mikaeel Kassam, Srikant Chakravarthi, Austin Epping, Sarah Erpenbeck, Maharaj Singh, Melanie B. Fukui, Amin Kassam, Richard Rovin

School of Dentistry Faculty Research and Publications

Introduction

Evolution of optical technology from two-dimensional to three-dimensional (3D) systems has come with an associated loss of stereoscopy and 3D depth perception. This report compares performance of surgical tasks in unbiased subjects using these systems.

Methods

Untrained subjects were randomized into two groups, robotically operated video optical telescopic-microscope (ROVOT) or surgical microscope (microscope). Subjects sutured and tied knots. Completion time, NASA-Task Load Index (TLX), and galvanic skin responses were analyzed.

Results

Intergroup analysis of suture completion time indicated that microscope use was significantly faster compared to ROVOT, whether used first or second. Regardless of which methodology was used first, …

Three-Dimensional Bio-Printing And Bone Tissue Engineering: Technical Innovations And Potential Applications In Maxillofacial Reconstructive Surgery, Muhja Salah, Lobat Tayebi, Keyvan Moharamzadeh, Farhad B. Naini

School of Dentistry Faculty Research and Publications

Background

Bone grafting has been considered the gold standard for hard tissue reconstructive surgery and is widely used for large mandibular defect reconstruction. However, the midface encompasses delicate structures that are surrounded by a complex bone architecture, which makes bone grafting using traditional methods very challenging. Three-dimensional (3D) bioprinting is a developing technology that is derived from the evolution of additive manufacturing. It enables precise development of a scaffold from different available biomaterials that mimic the shape, size, and dimension of a defect without relying only on the surgeon’s skills and capabilities, and subsequently, may enhance surgical outcomes and, in …

Effect Of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium On Cerulein-Induced Acute Pancreatitis In Mice, Kamal Abdolmohammadi, Tayebeh Mahmoudi, Shahrzad Nojehdehi, Lobat Tayebi, Seyed Mahmoud Hashemi, Farshid Noorbakhsh, Alireza Abdollahi, Masoud Soleimani, Behrouz Nikbin, Mohammad Hossein Nicknam

School of Dentistry Faculty Research and Publications

Purpose: Acute pancreatitis (AP) is an inflammatory disorder distinguished by tissue injury and inflammation of the pancreas. Using paracrine potential of mesenchymal stem cells (MSCs) provides a useful clinical approach in treating inflammatory diseases. We investigated the therapeutic effects of adipose-derived MSC conditioned medium (CM) and hypoxia preconditioned adipose-derived MSC conditioned medium (HCM) in cerulein-induced AP in mice.

Methods: AP was induced in C57BL/6 mice by intraperitoneal injection of cerulein (75 μg/ kg/h × 7 times). One hour following the last injection of cerulein, mice were treated with intraperitoneal injection of CM and HCM (500 µL/mice/30 min × 3 times). …

Role Of Iron On Physical And Mechanical Properties Of Brushite Cements, And Interaction With Human Dental Pulp Stem Cells, Sahar Vahabzadeh, Sarah Fleck, Joshua Marble, Fahimeh Tabatabaei, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Improving the physical, mechanical and biological properties of brushite cements (BrC) is of a great interest for using them in bone and dental tissue engineering applications. The objective of this study was to incorporate iron (Fe) at different concentrations (0.25, 0.50, and 1.00 wt%) to BrC and study the role of Fe on phase composition, setting time, compressive strength, and interaction with human dental pulp stem cells (hDPSCs). Results showed that increase in Fe concentration increases the β-tricalcium phosphate (β-TCP)/dicalcium phosphate dihydrate (DCPD) ratio and prolongs the initial and final setting time due to effective role of Fe on stabilizing …