Dentistry Commons^™

In Vitro And In Vivo Effects Of Concentrated Growth Factor On Cells And Tissues, Fahimeh Tabatabaei, Zahra Aghamohammadi, Lobat Tayebi

School of Dentistry Faculty Research and Publications

This article reviews the biological outcome of the concentrated growth factor (CGF), a new platelet derivative used for tissue regeneration, in published articles related to the use of this product in basic and clinical studies. An electronic literature research using PubMed and SCOPUS was performed using combination of keywords: “concentrated growth factor” (OR “CGF”), AND “stem cells,” AND “cells” OR “cell proliferation” OR “cell migration” OR “cell differentiation,” AND “repair” OR “survival” OR “revitalization,” AND “tissue” OR “bone.” Forty-five articles that were published between 2012 and 2020 met the inclusion criteria. These studies have used CGF as fresh solid form, …

Extra Nodal Rosai-Dorfman Disease Originating In The Nasal And Paranasal Complex And Gnathic Bones: A Systematic Analysis Of Seven Cases And Review Of Literature, Junu Ojha, Yeshwant B. Rawal, Jason L. Hornick, Kelly Magliocca, David R. Montgomery, Robert D. Foss, Kevin R. Torske, Brent Accurso

School of Dentistry Faculty Research and Publications

Rosai-Dorfman disease (RDD) is a benign, self-limiting histiocytosis of unknown etiology. The classic form of the condition includes a painless cervical lymphaenopathy [sic] accompanied by fever, weight loss and an elevated ESR. Extra nodal RDD (ENRDD) is most frequent in the head and neck. Thirty-eight cases of ENRDD have been described. Seven cases of ENRDD were identified in our pathology biopsy services. The demographic and clinical information was tabulated logically on the basis of age, gender, location and presence or absence of symptoms, treatment and follow-up. Radiographic and histopathological features were also examined. The findings in these cases were correlated …

Biomedical Applications Of Tio2 Nanostructures: Recent Advances, Sevda Jafari, Baharak Mahyad, Hadi Hashemzadeh, Sajjad Janfaza, Tooba Gholikhani, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Titanium dioxide (TiO₂) nanostructures are one of the most plentiful compounds that have emerged in various fields of technology such as medicine, energy and biosensing. Various TiO₂ nanostructures (nanotubes [NTs] and nanowires) have been employed in photoelectrochemical (PEC) biosensing applications, greatly enhancing the detection of targets. TiO₂ nanostructures, used as reinforced material or coatings for the bare surface of titanium implants, are excellent additive materials to compensate titanium implants deficiencies—like poor surface interaction with surrounding tissues—by providing nanoporous surfaces and hierarchical structures. These nanostructures can also be loaded by diversified drugs—like osteoporosis drugs, anticancer and antibiotics—and …

Manufacturing And Characterization Of Mechanical, Biological And Dielectric Properties Of Hydroxyapatite-Barium Titanate Nanocomposite Scaffolds, Mina Tavangar, Fatemeh Heidari, Raziye Hayati, Fahimeh Tabatabaei, Daryoosh Vashaee, Lobat Tayebi

School of Dentistry Faculty Research and Publications

In this research, hydroxyapatite (HA)-based ceramics were produced as suitable ceramic implants for orthopedic applications. To improve the physical, mechanical, electrical and biological properties of pure HA, we developed composite scaffolds of HA-barium titanate (BT) by cold isostatic pressing and sintering. Microstructure, crystal phases, and molecular structure were analyzed by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Bulk density values were measured using the Archimedes method. The effect of different percentages of BT on cell proliferation, viability, and ALP activity of dental pulp stem cells (DPSCs) was assessed …

The Incidence Of Root Canal Therapy After Full-Coverage Restorations: A 10-Year Retrospective Study, Abby Yavorek, Pradeep Bhagavatula, Kadam Patel, Aniko Szabo, Mohamed Ibrahim

School of Dentistry Faculty Research and Publications

Introduction

The process of restoring a tooth with a crown leaves many opportunities for pulpal irritation. The objective of this study was to identify and analyze the factors that contribute to the incidence of nonsurgical root canal therapy (NS-RCT) after the delivery of single-unit full-coverage restorations.

Methods

Insurance claims from 88,409 crown placements in the Delta Dental of Wisconsin insurance database were analyzed from the years 2008–2017. The Cox regression model was used to analyze the effect of the predictor variables on the survival of the tooth. Untoward events were defined as NS-RCT, tooth extraction, retreatment of root canal, or …

Bismuth-Based Nanomaterials: Recent Advances In Tumor Targeting And Synergistic Cancer Therapy Techniques, Samireh Badrigilan, Jalal Choupani, Hashem Khanbabaei, Mojtaba Hoseini-Ghahfarokhi, Thomas J. Webster, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Despite all of the efforts in the field of cancer therapy, the heterogeneous properties of tumor cells induce an insufficient therapeutic outcome when treated with conventional monotherapies, necessitating a shift in cancer treatment from monotherapy to combination therapy for complete cancer treatment. Multifunctional bismuth (Bi)-based nanomaterials (NMs) with therapeutic functions hold great promise for the fields of cancer diagnosis and therapy based on their low toxicity, X-ray sensitive capabilities, high atomic number, near-infrared driven semiconductor properties, and low cost. Herein, a comprehensive review of recent advances in various medicinal aspects of Bi-based NMs is presented including: evaluation of in-tumor site …

Turkish Paediatric Dentists’ Knowledge, Experiences And Attitudes Regarding Child Physical Abuse, Nermin Özgür, Elif Ballikaya, H. Cem Güngör, Atilla S. Ataç

School of Dentistry Faculty Research and Publications

Objectives

Numerous studies have shown that the education of health professionals is essential to effectively respond to child abuse. The present study aimed to evaluate Turkish paediatric dentists' knowledge, experiences and attitudes regarding child physical abuse.

Materials and methods

An electronic questionnaire was e‐mailed to 518 paediatric dentists. Participants’ knowledge of diagnostic indicators of child physical abuse, and their past experiences, attitudes and self‐assessment of educational needs were evaluated.

Results

The response rate was 40.9% (n = 212). Participants who completed their undergraduate education before 1997 received significantly less education on child physical abuse than participants who completed their …

Vibrational And Sonochemical Characterization Of Ultrasonic Endodontic Activating Devices For Translation To Clinical Efficacy, Erfan Dashtimoghadam, Alexander Johnson, Farahnaz Fahimipour, Mohamadali Malakoutian, Jessica Vargas, Jose Gonzalez, Mohamed Ibrahim, John Baeten, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Passive activation of endodontic irrigants provides improved canal disinfection, smear layer removal, and better subsequent sealing. Although evidence suggests that passive activating endodontic devices increase the effectiveness of irrigation, no study exists to quantitatively compare and validate vibrational characteristics and cavitation produced by different ultrasonic endodontic devices. The current study aims to compare the efficiency of various commercially available ultrasonic endodontic activating devices (i.e., EndoUltra™, EndoChuck, Irrisafe™, and PiezoFlow®). The passive endodontic activating devices were characterized in terms of tip displacement and cavitation performance using scanning laser vibrometry (SLV) and sonochemical analysis, respectively. The obtained results showed that …

Critical-Sized Bone Defects Regeneration Using A Bone-Inspired 3d Bilayer Collagen Membrane In Combination With Leukocyte And Platelet-Rich Fibrin Membrane (L-Prf): An In Vivo Study, Farahnaz Fahimipour, Farshid Bastami, Ahad Khoshzaban, Shahrbanoo Jahangir, Mohamadreza Baghaban Eslaminejad, Fahimeh Kahyyatan, Hannaneh Safiaghdam, Yeganeh Sadooghi, Majid Safa, Tahereh S. Jafarzadeh Kashi, Erfan Dashtimoghadam, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Objectives

We aim to develop a 3D-bilayer collagen (COL) membrane reinforced with nano beta-tricalcium-phosphate (nβ-TCP) particles and to evaluate its bone regeneration in combination with leukocyte-platelet-rich fibrin (L-PRF) in vivo.

Background data

L-PRF has exhibited promising results as a cell carrier in bone regeneration in a number of clinical studies, however there are some studies that did not confirm the positive results of L-PRF application.

Methods

Mechanical & physiochemical characteristics of the COL/nβ-TCP membrane (1/2 & 1/4) were tested. Proliferation and osteogenic differentiation of seeded cells on bilayer collagen/nβ-TCP thick membrane was examined. Then, critical-sized calvarial defects in 8 …

Incorporation Of Functionalized Reduced Graphene Oxide/Magnesium Nanohybrid To Enhance The Osteoinductivity Capability Of 3d Printed Calcium Phosphate-Based Scaffolds, Meisam Omidi, Hossein Golzar, Dorsa Mohammadrezaei, Amir Yadegari, Morteza Rasoulianboroujeni, Mohadeseh Hashemi, Fatemeh Yazdian, Mohammad Shalbaf, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Improving bone regeneration is one of the most pressing problems facing bone tissue engineering (BTE) which can be tackled by incorporating different biomaterials into the fabrication of the scaffolds. The present study aims to apply the 3D-printing and freeze-drying methods to design an ideal scaffold for improving the osteogenic capacity of Dental pulp stem cells (DPSCs). To achieve this purpose, hybrid constructs consisted of 3D-printed Beta-tricalcium phosphate (β-TCP)-based scaffolds filled with freeze-dried gelatin/reduced graphene oxide-Magnesium-Arginine (GRMA) matrix were fabricated through a novel green method. The effect of different concentrations of Reduced graphene oxide-Magnesium-Arginine (RMA) (0, 0.25% and 0.75%wt) on the …

Structure, Wettability, Corrosion And Biocompatibility Of Nitinol Treated By Alkaline Hydrothermal And Hydrophobic Functionalization For Cardiovascular Applications, S. Rahimipour, E. Salahinejad, E. Sharifi, H. Nosrati, Lobat Tayebi

School of Dentistry Faculty Research and Publications

The main objective of this study is to hydrophobize nitinol (Ni-Ti alloy) for cardiovascular applications. For this purpose, medical nitinol samples were subjected to sodium hydroxide hydrothermal treatments at various temperatures, followed by hexadecyltrimethoxysilane (HDTMS) functionalization. Then, the structure, wettability, corrosion, cytocompatibility and cell adhesion of the prepared samples were evaluated. According to the results, porous blade-shaped layers of sodium titanate were formed on the substrate surface as a result of the alkaline treatment. These nano-rough features offered considerable hydrophobicity after HDTMS processing, where a maximum water contact angle of about 140° was obtained for the sample treated at 120 …

Mechanistic Assessment Of Functionalized Mesoporous Silica-Mediated Insulin Fibrillation, Mohsen Akbarian, Lobat Tayebi, Soliman Mohammadi-Samani, Fatemeh Farjadian

School of Dentistry Faculty Research and Publications

Insulin, which is a small protein hormone consisting of 51 amino acids, rapidly fibrillates under stressogenic conditions. This biotechnological/ medical problematic reaction quickly accelerates in the presence of some particles, while there are several other particles that slow down the kinetic process. To address the unexplored demand of the particles that modulate protein fibrillation, we have synthesized two amino-based particles and a chitosan-coated mesoporous silica particle (MS-NH₂, MS-3NH₂, and MS-chitosan) to investigate insulin fibrillation. While these particles were fairly similar in size, they are differ in their net positive charge and surface hydrophobicity. To monitor the …

Biodegradable Magnesium Bone Implants Coated With A Novel Bioceramic Nanocomposite, Mehdi Razavi, Mohammadhossein Fathi, Omid Savabi, Lobat Tayebi, Daryoosh Vashaee

School of Dentistry Faculty Research and Publications

Magnesium (Mg) alloys are being investigated as a biodegradable metallic biomaterial because of their mechanical property profile, which is similar to the human bone. However, implants based on Mg alloys are corroded quickly in the body before the bone fracture is fully healed. Therefore, we aimed to reduce the corrosion rate of Mg using a double protective layer. We used a magnesium-aluminum-zinc alloy (AZ91) and treated its surface with micro-arc oxidation (MAO) technique to first form an intermediate layer. Next, a bioceramic nanocomposite composed of diopside, bredigite, and fluoridated hydroxyapatite (FHA) was coated on the surface of MAO treated AZ91 …

Mmp-7 And Mmp-9 Are Overexpressed In The Synovial Tissue From Severe Temporomandibular Joint Dysfunction, Carla Loreto, Veronica Filetti, Luis Eduardo Almeida, Giusy Rita Maria La Rosa, Rosalia Leonardi, Cristina Grippaudo, Antonino Lo Giudice

School of Dentistry Faculty Research and Publications

Matrix metalloproteinases (MMPs) are tissue-enzymes that play a key role during the remodeling process, such as in inflammatory diseases. MMP-7 and MMP-9 have been shown to be implicated in extracellular matrix homeostasis and in joint disc remodeling. The objective of this study was to determine the relation of MMP-7 and MMP-9 expression with severe temporomandibular joint dysfunction, in particular with anterior disk displacement without reduction (ADDwoR), using an immunohistochemical approach. Therefore, twenty human temporomandibular synovia in the test group and ten in the control group were collected. The results showed there was a statistically significant difference (P<0.001) for morphometric and densitometric analysis of both detected MMPs in control and test groups. In conclusion, MMP-7 and MMP-9 were overexpressed in the synovial tissue of patients with ADDwoR.

A Ferrofluidic Hydrophobic Deep Eutectic Solvent For The Extraction Of Doxycycline From Urine, Blood Plasma And Milk Samples Prior To Its Determination By High-Performance Liquid Chromatography-Ultraviolet, Ebrahim Alipanahpour Dil, Mehrorang Ghaedi, Arash Asafram, Lobat Tayebi, Fatemah Mehrabi

School of Dentistry Faculty Research and Publications

The ferrofluid phase was prepared according to mixing magnetic nanoparticle and the hydrophobic deep eutectic solvent as a green microextraction solvent. This new composite was applied for vortex-assisted dispersive liquid–liquid microextraction (VA-HDES-ferrofluid-DLLME) of doxycycline (DOC) residual extraction and determined through high-performance liquid chromatography-ultraviolet (HPLC-UV). The characterization of magnetic nanoparticle was investigated by XRD, TEM and FESEM. The dependency of DOC microextraction to main variables and their interaction and find optimum points were undertaken using response surface methodology with either central composite design (CCD). Thus, the optimum pH, ionic strength, ferrofluid volume and vortex time for DOC extraction are determined to …

Spontaneous Condyle-Like Development After Total Resection Of Mandible Giant Osteochondroma: Case Report And A Follow-Up For Five Years, Marco Antonio De Oliveira Filho, Luis Eduardo Almeida, Andrea Doetzer, Allan Giovanini, Osvaldo Malafaia

School of Dentistry Faculty Research and Publications

Osteochondroma manifests as a benign tumor that occurs as an abnormal bony development. This tumor is commonly asymptomatic and presents an exophytic outgrowth on bone surfaces, near synovial joints, a condition that invariably induces evident facial deformities. Treatment for this type of tumor usually involves a surgical approach promoting a total or partial resection of the affected anatomical area associated to prosthetic reconstruction of the bone area extracted. We present a case report about a giant mandibular condyle osteochondroma in a 37-year-old female patient. Her treatment involved a total condylectomy without immediate condylar reconstruction, which would be performed in a …

Recent Advances In Porphyrin-Based Nanocomposites For Effective Targeted Imaging And Therapy, Navid Rabiee, Mohammad Tavakkoli Yaraki, Soha Mokhtari Garakani, Shima Mokhtari Garakani, Sepideh Ahmadi, Aseman Lajevardi, Mojtaba Bagherzadeh, Mohammad Rabiee, Lobat Tayebi, Mohammadreza Tahriri, Michael R. Hamblin

School of Dentistry Faculty Research and Publications

Porphyrins are organic compounds that continue to attract much theoretical interest, and have been called the “pigments of life”. They have a wide role in photodynamic and sonodynamic therapy, along with uses in magnetic resonance, fluorescence and photoacoustic imaging. There is a vast range of porphyrins that have been isolated or designed, but few of them have real clinical applications. Due to the hydrophobic properties of porphyrins, and their tendency to aggregate by stacking of the planar molecules they are difficult to work with in aqueous media. Therefore encapsulating them in nanoparticles (NPs) or attachment to various delivery vehicles have …

Polymeric Scaffolds For Dental Pulp Tissue Engineering: A Review, Hossein E. Jazayeri, Su-Min Lee, Lauren Kuhn, Farahnaz Fahimipour, Mohammadreza Tahriri, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Objectives

The purpose of this review is to describe recent developments in pulp tissue engineering using scaffolds and/or stem cells. It is crucial to understand how this approach can revitalize damaged dentin-pulp tissue. Widespread scaffold materials, both natural and synthetic, and their fabrication methods, and stem-progenitor cells with the potential of pulp regeneration will be discussed.

Data and Sources

A review of literature was conducted through online databases, including MEDLINE by using the PubMed search engine, Scopus, and the Cochrane Library.

Study Selection

Studies were selected based on relevance, with a preference given to recent research, particularly from the past …

In Vitro Evaluation Of Different Protocols For Preventing Microleakage Of Fissure Sealants Placed Following Saliva Contamination, Hayrunnisa Şimşek, A. Rüya Yazici, H. Cem Güngör

School of Dentistry Faculty Research and Publications

Purpose: To evaluate the effect of different enamel conditioning protocols and their re-application on the microleakage of fissure sealants placed following saliva contamination.

Study design: The study included 156 human third molars in 16 subgroups (2×4×2) under two main groups (sealant type): Group A- hydrophobic resin sealant, 3M Clinpro^™ Sealant; Group B- hydrophilic resin sealant, Ultraseal XT Hydro. Each group was then divided according to the type of surface conditioning; 1- Er,Cr:YSSG laser etching, 2- acid-etching, 3- acid-etching+etch-and-rinse adhesive (Prime&Bond^® One Select) and 4- self-etching adhesive (Clearfil^™SE Bond). After contaminating the conditioned occlusal enamel surfaces with …

Current Trends And Future Perspectives Of Oral Pathology: Indian Scenario, Yeshwant B. Rawal

School of Dentistry Faculty Research and Publications

No abstract provided.

Multitarget Stool Dna Testing For The Prevention Of Colon Cancer: Outcomes In A Large Integrated Healthcare System, Nimish Vakil, Kristin Ciezki, Nadia Huq, Maharaj Singh

School of Dentistry Faculty Research and Publications

Background and Aims

Multitarget stool DNA (MT-sDNA) testing is used in primary care as a screening test for colon cancer. Test effectiveness and patient compliance were examined in clinical practice.

Methods

We assessed outcomes of MT-sDNA testing in a cohort study conducted in a large integrated healthcare system comprising 15 hospitals and 150 outpatient clinics using advanced electronic data capture (Clarity2 [Epic, Verona, Wisc, USA] and REDCap [Encinitas, Calif, USA]) followed by manual chart review to confirm MT-sDNA test results and to monitor the outcomes of subsequent colonoscopy.

Results

A total of 6835 MT-sDNA tests were performed over 1 year …

Hydrogen Peroxide Preconditioning Promotes Protective Effects Of Umbilical Cord Vein Mesenchymal Stem Cells In Experimental Pulmonary Fibrosis, Tayebeh Mahmoudi, Kamal Abdolmohammadi, Hamed Bashiri, Mehdi Mohammadi, Mohammad Jafar Rezaie, Fardin Fathi, Shohreh Fakhari, Mohammad Ali Rezaee, Ali Jalili, Mohammad Reza Rahmani, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Purpose

Idiopathic pulmonary fibrosis (IPF) is a progressive lung disorder with few available treatments. Mesenchymal stem cell therapy (MSCT), an innovative approach, has high therapeutic potential when used to treat IPF. According to recent data, preconditioning of MSCs can improve their therapeutic effects. Our research focuses on investigating the anti-inflammatory and antifibrotic effects of H₂O₂-preconditioned MSCs (p-MSCs) on mice with bleomycin-induced pulmonary fibrosis (PF).

Methods

Eight-week-old male C57BL/6 mice were induced with PF by intratracheal (IT) instillation of bleomycin (4 U/kg). Human umbilical cord vein-derived MSCs (hUCV-MSCs) were isolated and exposed to a sub-lethal concentration (15 …

Biomedical Applications Of Zeolitic Nanoparticles, With An Emphasis On Medical Interventions, Hossein Derakhshankhah, Samira Jafari, Sajad Sarvari, Ebrahim Barzegari, Faezeh Moakedi, Milad Ghorbani, Behrang Shiri Varnamkhasti, Mehdi Jaymand, Zhila Izadi, Lobat Tayebi

School of Dentistry Faculty Research and Publications

The advent of porous materials, in particular zeolitic nanoparticles, has opened up unprecedented putative research avenues in nanomedicine. Zeolites with intracrystal mesopores are low framework density aluminosilicates possessing a regular porous structure along with intricate channels. Their unique physiochemical as well as physiological parameters necessitate a comprehensive overview on their classifications, fabrication platforms, cellular/macromolecular interactions, and eventually their prospective biomedical applications through illustrating the challenges and opportunities in different integrative medical and pharmaceutical fields. More particularly, an update on recent advances in zeolite-accommodated drug delivery and the prevalent challenges regarding these molecular sieves is to be presented. In conclusion, strategies …

A Glassy Carbon Electrode Modified With Reduced Graphene Oxide And Gold Nanoparticles For Electrochemical Aptasensing Of Lipopolysaccharides From Escherichia Coli Bacteria, Mehrab Pourmadadi, Javad Shabani Shayeh, Meisam Omidi, Fatemeh Yazdian, Masoud Alebouyeh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

An electrochemical aptasensor is described for the voltammetric determination of lipopolysaccharide (LPS) from Escherichia coli 055:B5. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via reduced graphene oxide and gold nanoparticles (RGO/AuNPs). Fast Fourier transform infrared, X-ray diffraction and transmission electron microscopy were used to characterize the nanomaterials. Cyclic voltammetry, square wave voltammetry and electrochemical impedance spectroscopy were used to characterize the modified GCE. The results show that the modified electrode has a good selectivity for LPS over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) is used as …

Conductive Nanofibrous Chitosan/Pedot:Pss Tissue Engineering Scaffolds, Ali Abedi, Mahdi Hasanzadeh, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Design of a proper scaffold is the first step in fabrication of a tissue engineering product, which should be able to support cellular growth in in vitro conditions. This study focuses on the fabrication and characterization of chitosan (CS) scaffolds containing PEDOT:PSS, a conductive polymer. The scaffold is primarily designed for cardiac tissue engineering, although it can be used for other applications too. Chitosan scaffolds containing 0.3, 0.6 and 1 wt% of PEDOT:PSS are fabricated through electrospinning. The structure and morphology of scaffolds are characterized by scanning electron microscopy (SEM), 3D Laser Measuring Microscopy and Fourier-transform infrared spectroscopy (FTIR). The …

Synthesis And Application Of Ce-Doped Tio2 Nanoparticles Loaded On Activated Carbon For Ultrasound-Assisted Adsorption Of Basic Red 46 Dye, Ebrahim Alipanahpour Dil, Mehrorang Ghaedi, Arash Asfaram, Fatemah Mehrabi, Ali Akbar Bazrafshan, Lobat Tayebi

School of Dentistry Faculty Research and Publications

Ce was doped on TiO₂ nanoparticles (NPs), and this association was loaded on activated carbon (Ce-TiO₂-NPs-AC). The characterization was completed by FE-SEM, TEM, and XRD, and finally these NPs were used for the ultrasonic-assisted adsorption of Basic Red 46 (BR 46) from aqueous solution. An experimental model suggested by the central composite design (CCD)—as a branch of response surface methodology (RSM)—provides insight into the influence of variables, such as BR 46 concentration, pH, adsorbent mass, and sonication time, on BR 46 removal. Experimental results revealed that setting conditions at 25 mg L⁻¹ of BR 46, pH …

Design Of A New 3d‐Printed Joint Plug, Lobat Tayebi, Zhanfeng Cui, Hua Ye

School of Dentistry Faculty Research and Publications

This paper introduces a kit of parts as a novel three‐dimensional (3D)–printed joint plug, in which each of the parts function cooperatively to treat cartilage damage in joints of the human body (e.g., hips, wrists, elbow, knee, and ankle). Three required and one optional parts are involved in this plug. The first part is a 3D‐printed hard scaffold (bone portion) to accommodate bone cells, and the second is a 3D‐printed soft scaffold (cartilage portion) overlying the bone portion to accommodate chondrocytes. The third part of joint plug is a permeable membrane, termed film, to cover the entire plug to provide …

Matrix Metalloproteinases And Temporomandibular Joint Disorder: A Review Of The Literature, Logan Herm, Ardit Haxhia, Flavio Camejo, Lobat Tayebi, Luis Eduardo Almeida

School of Dentistry Faculty Research and Publications

Temporomandibular disorders (TMD) are progressive degenerative disorders that affect the components of the temporomandibular joint (TMJ), characterized by pain and limitations in function. Matrix metalloproteinases (MMP) are enzymes involved in physiological breakdown of tissue that can have a pathological effect from an increase in activity during inflammation. A PubMed search of the current literature (within the past 10 years) was conducted to identify human studies involving matrix metalloproteinases activity in TMJ components of patients with TMD. Two separate searches results in 34 studies, six of which met inclusion criteria. Immunohistochemistry and gene analysis were used to evaluate MMP expression in …

A Graphene Based–Biomimetic Molecularly Imprinted Polyaniline Sensor For Ultrasensitive Detection Of Human Cardiac Troponin T(Ctnt), Meysam Karimi, Mohammad Rabiee, Mohammadreza Tahriri, Reza Salarian, Lobat Tayebi

School of Dentistry Faculty Research and Publications

In the present work, a biomimetic nano-molecularly imprinted polymer (N-MIP) electrode based on a graphene screen-printed electrode was developed for the ultrasensitive detection of cardiac troponin T (cTnT). The biomimetic cavities for targeted sensing for analyte were fabricated by the electropolymerization of conductive co-polymer matrix of aniline and carboxylated aniline on the graphene oxide (GO) electrode, in the presence template protein (cTnT for cardiac troponin T probe) by cyclic voltammetry. The surface characterization of the sensor was performed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), and scanning electron microscopy (SEM). The best biomimetic surface nanotexture was obtained at …

Gene Therapy In Rheumatoid Arthritis: Strategies To Select Therapeutic Genes, Mahdi Zavvar, Sara Assadiasl, Narjes Soleimanifar, Fatemeh Dadgar Pakdel, Kamal Abdolmohammadi, Yousef Fatahi, Mohsen Abdolmaleki, Hamed Baghdadi, Lobat Tayebi, Mohammad Hossein Nicknam

School of Dentistry Faculty Research and Publications

Significant advances have been achieved in recent years to ameliorate rheumatoid arthritis (RA) in animal models using gene therapy approaches rather than biological treatments. Although biological agents serve as antirheumatic drugs with suppressing proinflammatory cytokine activities, they are usually accompanied by systemic immune suppression resulting from continuous or high systemic dose injections of biological agents. Therefore, gene transfer approaches have opened an interesting perspective to deliver one or multiple genes in a target‐specific or inducible manner for the sustained intra‐articular expression of therapeutic products. Accordingly, many studies have focused on gene transferring methods in animal models by using one of …