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Articles 1 - 4 of 4
Full-Text Articles in Cells
Microfabrication Of An Optical Chamber For Exposing Living Cells To Pulsed Electric Fields, Saleh Sheikh
Microfabrication Of An Optical Chamber For Exposing Living Cells To Pulsed Electric Fields, Saleh Sheikh
Electrical & Computer Engineering Theses & Dissertations
I have fabricated a chamber which enables the application of Nanosecond Pulsed Electric Fields (nsPEFs) to living cells while monitoring their real time fluorescence with an inverted microscope. The No. I thickness (150µm nominal) coverslip glass substrate used for fabrication allows the use of high numerical aperture oil immersion objectives for imaging intracellular organelles. Electrodes are fabricated by first evaporating a chromium adhesion layer, followed by a nickel seed layer. Subsequently, an ultra-thick positive photoresist is applied and patterned to create a 30µm thick electroplating mold. The electrodes are then electrodeposited into the mold from a nickel sulfamate plating bath. …
Probing The Role Of Nascent Helicity In Protein Function: P27kip1 As A Regulator Of The Cell Cycle, Steve Otieno
Probing The Role Of Nascent Helicity In Protein Function: P27kip1 As A Regulator Of The Cell Cycle, Steve Otieno
Theses and Dissertations (ETD)
p27kip1 binds to and regulates the activity of cyclin-dependent kinases (Cdks) which are the master timekeepers of the cell division cycle. Members of the p27 family of proteins, also including p21 and p57, are called cyclin-dependent kinase inhibitors (CKIs). The amino terminal domain of p27 inhibits Cdk activity and is referred to as the kinase inhibitory domain (KID). The KID is comprised of a cyclin-binding domain (D1) and a Cdk binding domain (D2) joined by a 22 residue linker domain (LH). Structural analysis of the KID in solution before binding its Cdk targets revealed that D1 and D2 are …
Fibrocystin/Polyductin, Found In The Same Protein Complex With Polycystin-2, Regulates Calcium Responses In Kidney Epithelia, Shizuan Wang, Jingjing Zhang, Surya M. Nauli, Xiaogang Li, Patrick G. Starremans, Ying Luo, Kristina A. Roberts, Jing Zhou
Fibrocystin/Polyductin, Found In The Same Protein Complex With Polycystin-2, Regulates Calcium Responses In Kidney Epithelia, Shizuan Wang, Jingjing Zhang, Surya M. Nauli, Xiaogang Li, Patrick G. Starremans, Ying Luo, Kristina A. Roberts, Jing Zhou
Pharmacy Faculty Articles and Research
Recent evidence suggests that fibrocystin/polyductin (FPC), polycystin-1 (PC1), and polycystin-2 (PC2) are all localized at the plasma membrane and the primary cilium, where PC1 and PC2 contribute to fluid How sensation and may function in the same mechanotransduction pathways. To further define the exact subcellular localization of FPC, the protein product encoded by the PKHD1 gene responsible for autosomal recessive polycystic kidney disease (PKD) in humans, and whether FPC has direct and/or indirect cross talk with PC2, which, in turn, is pivotal for the pathogenesis of autosomal dominant PKD, we performed double immunostaining and coimmunoprecipitation as well as a microfluorimetry …
The Expression And Function Of Ppar And Hif-1 In Human Melanoma, Caroline Mills
The Expression And Function Of Ppar And Hif-1 In Human Melanoma, Caroline Mills
Theses, Dissertations and Capstones
The first part of my dissertation focuses on the expression and function of PPARs in human melanoma. I found that the A375 cells were significantly growth inhibited in response to PGJ2 and troglitazone treatment. HEMn-LP showed significant growth inhibition in response to troglitazone. I found that PPARγ and PPARδ mRNA is present in both the SK-Mel 28 and A375 cells. The relative level of PPARα mRNA expression is highest in SK-Mel 28 cells, ~3 fold higher relative to both the normal human melanocytes and A375 cells. PPARγ protein was ~50% higher in both SK-Mel 28 and A375 cells relative to …