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Full-Text Articles in Cells
Inducible Gene Expression In Cell Cultures And In Microencapsulated Cells, Yong Yu
Inducible Gene Expression In Cell Cultures And In Microencapsulated Cells, Yong Yu
Loma Linda University Electronic Theses, Dissertations & Projects
The mifepristone-regulated transcriptional activation system provides a means to exogenously control gene expression in transformed mammalian cells in a temporal and spatial fashion. Such a promoter activation system consists of three components: The first of these is a steroidal inducer drug, mifepristone. This drug binds to the second component, a chimeric transcription factor complex, consisting of the mutant human progesterone receptor fused to the yeast GAL4 DNA-binding domain and the Herpes simplex virus protein VP 16 activation domain. The third component is a synthetic promoter, consisting of a series of GAL4 recognition sequences upstream of the adenovirus major late E1B …
Regulation Of The Insulin-Like Growth Factor System By Retinoic Acid In Human Osteoblast Cells, Yuehua Zhou
Regulation Of The Insulin-Like Growth Factor System By Retinoic Acid In Human Osteoblast Cells, Yuehua Zhou
Loma Linda University Electronic Theses, Dissertations & Projects
Retinoic acid (RA) is an important regulator of growth and differentiation in many cell types, including bone. However, its effects on human osteoblast cell growth and differentiation have not been well studied. Therefore, I investigated the effect of RA on proliferation and differentiation of normal human bone cells (HBCs) and human osteosarcoma, SaOS-2 cells. RA decreased baseline as well as serum-stimulated proliferation in normal HBCs. To determine the effect of RA on differentiation, expression of several osteoblastic differentiation markers were studied. RA decreased type I procollagen mRNA levels and l,25(OH)2D3-stimulated osteocalcin and alkaline phosphatase (ALP) mRNA …
Stab Wounds To Rat Brains : Demyelination, Remyelination And The Cellular Response, Particularly Of Oligodendrocytes, Xie Dangci
Loma Linda University Electronic Theses, Dissertations & Projects
The present body of knowledge concerning the results of central nervous system injury is incomplete and controversial. The role of oligodendrocytes, the cells involved in phagocytosis, the stages of demyelination, and the evidence for remyelination are of interest. Following stab wounds to rat brain cortex, corpus callosum and hippocampus, the animals were examined at 4,7,11,18,23,29,45,60 and 70 days postoperation (dpo). To help in the identification of cells immunocytochemical markers for astrocytes and immature oligodendrocytes were used. These were labels for glial fibrillary acidic protein (GFAP) and myelin/oligodendrocyte specific protein (MOSP) respectively. Light and electron microscopy were correlated using conventionally prepared …