Medicine and Health Sciences Commons^™

Effects Of Superovulated Heifer Diet Type And Quantity On Relative Mrna Abundances And Pyruvate Metabolism In Recovered Embryos., C Wrenzycki, P De Sousa, E W Overström, R T Duby, D Herrmann, A J Watson, H Niemann, D O'Callaghan, M P Boland

Obstetrics & Gynaecology Publications

This study investigated the effects of quantity and type of diet fed to superovulated donor heifers on molecular and metabolic indices of embryonic development. These effects included the relative abundances of mRNAs for the alpha 1 subunit of Na/K-ATPase and the antioxidant enzyme Cu/Zn-SOD, as well as pyruvate utilization in bovine morulae and blastocysts developed in vivo. Heifers were fed a daily ration of either grass silage and a citrus-beet pulp-based concentrate or grass silage and a barley-based concentrate for 116 days, both at 3 kg per day or ad libitum. In embryos derived from heifers fed the pulp-based diets, …

Acute Pneumonia Associated With Chlamydiapneumoniae Infection: A Case Report, S. Likitnukul, P. Nunthapisud, S. Tumwasorn

Chulalongkorn Medical Journal

No abstract provided.

Gallstone Pancreatitis: An Update, S. Treeprasertsuk, P. Tangkijvanich

Chulalongkorn Medical Journal

No abstract provided.

New Era Of National Higher Education, N. Kamolvarin

Chulalongkorn Medical Journal

No abstract provided.

Four Clinical Tests Of Sacroiliac Joint Dysfunction: The Association Of Test Results With Innominate Torsion Among Patients With And Without Low Back Pain, Pamela Levangie

All PTHMS Faculty Publications

Background and Purpose. The purpose of this study was to assess the association between innominate torsion (asymmetric anteroposterior positioning of the pelvic innominates) and the Gillet, standing forward flexion, sitting forward flexion, and supine-to-sit tests. Subjects. A sample of 21- to 50-year-old patients with low back pain (n=150) and a comparison group of patients with upper-extremity impairments (n=138) were recruited from outpatient physical therapy facilities. Methods. The association of single and combined test results with innominate torsion (calculated from pelvic landmark data) and with presence or absence of low back pain were estimated via odds ratios, sensitivities, specificities, and predictive …

Physical Violence During Pregnancy: Maternal Complications And Birth Outcomes, Vilma E. Cokkinides, Ann L. Coker, Maureen Sanderson, Cheryl Addy, Lesa Bethea

CRVAW Faculty Journal Articles

Objective: To assess the association between physical violence during the 12 months before delivery and maternal complications and birth outcomes.

Methods: We used population-based data from 6143 women who delivered live-born infants between 1993 and 1995 in South Carolina. Data on women's physical violence during pregnancy were based on self-reports of partner-inflicted physical hurt and being involved in a physical fight. Outcome data included maternal antenatal hospitalizations, labor and delivery complications, low birth weights, and preterm births. Odds ratios and 95% confidence intervals were calculated to measure the associations between physical violence, maternal morbidity, and birth outcomes.

Results: The prevalence …

Pregnant Rat Uterus Expresses High Levels Of The Type 3 Iodothyronine Deiodinase, Valerie Anne Galton, Elena Martinez, Arturo Hernandez, Emily A. St. Germain, Joanne M. Bates, Donald L. St. Germain

Dartmouth Scholarship

Although thyroid hormones are critically important for the coordination of morphogenic processes in the fetus and neonate, premature exposure of the embryo to levels of the hormones present in the adult is detrimental and can result in growth retardation, malformations, and even death. We report here that the pregnant rat uterus expresses extremely high levels of the type 3 iodothyronine deiodinase (D3), which inactivates thyroxine and 3,3′,5-triiodothyronine by 5-deiodination. Both D3 mRNA and activity were present at the implantation site as early as gestational day 9 (E9), when expression was localized using in situ hybridization to uterine mesometrial and antimesometrial …

The Frequency Of Primary Ovarian Neoplasmsat King Chulalongkorn Memorial Hospitalduring 1990-1997, P. Trivijitsilp, S. Triratanachat, S. Niruthsard, K. Tantayaporn

Chulalongkorn Medical Journal

No abstract provided.

Igf Paracrine And Autocrine Interactions Between Conceptus And Oviduct., A J Watson, M E Westhusin, Q A Winger

Obstetrics & Gynaecology Publications

Development in vitro is influenced by embryo density, serum, somatic cell co-culture and the production of 'embryotrophic' paracrine and autocrine factors. Research in our laboratory has focussed principally on the insulin-like growth factor (IGF) family. We have demonstrated that pre-attachment bovine and ovine embryos express mRNAs encoding a number of growth factor ligand and receptor genes including all members of the IGF ligand and receptor family throughout this developmental interval. In addition, early embryos express mRNAs encoding IGF-binding proteins (IGFBPs) 2-5 from the one-cell to the blastocyst stage and IGFBP5 mRNA at the blastocyst stage. Cultured bovine blastocysts release up …

The Role Of Plasma Endothelin-1 In Preeclampsia, Yalçın Ki̇mya, Candan Cengi̇z, Hakan Ozan, Levent Büyükuysal, Sami Aydin, Serhat Tatlikazan

Turkish Journal of Medical Sciences

Endothelin-1 levels in normal and preeclamptic pregnant women were determined serially to investigate the correlation between renal function tests and the severity markers of preeclampsia. Plasma endothelin-1 levels were determined in 45 serial plasma samples from 15 pregnant women who subsequently developed preeclampsia, and in 43 plasma samples from 25 women with uncomplicated pregnancies retrospectively. Renal function tests, complete blood counts, urinalysis and blood pressure measurements were performed during antenatal follow-ups of these patients. No correlations were set between plasma endothelin-1 levels and renal function tests, thrombocyte and white blood cell counts, proteinuria, systolic and diastolic blood pressures. There was …

Aneuploidy Frequencies In Semen Fractions From Ten Oligoasthenoteratozoospermic Patients Donating Sperm For Intracytoplasmic Sperm Injection, Jerome Pfeffer, Myung-Geol Pang, Stanton F. Hoegerman, Christopher J. Osgood, M. W. Stacey, Jacob Mayer, Sergio Oehninger, William G. Kearns

Biological Sciences Faculty Publications

Objective: To determine aneuploidy frequencies in pellet and swim-up semen fractions from 10 infertile men with severe oligoasthenoteratozoospermia (OAT) who were donating sperm for intracytoplasmic sperm injection and to determine whether the swim-up isolation method would successfully separate aneuploid from haploid sperm.

Design: Prospective study.

Setting: Infertility clinic and molecular genetics laboratory.

Patient(s): Ten patients with severe OAT.

Intervention(s): Cytogenetic analyses by fluorescence in situ hybridization to determine aneuploidy frequencies for chromosomes 1, 13, 18, 21, X, and Y in sperm from swim-up and pellet fractions.

Main Outcome Measure(s): Gametic aneuploidy was scored in sperm fractions separated by the swim-up …

Erythrocyte, Saliva And Serum Arginase Activity With Serum Urea And Estrogen Levels Inakkaraman And Ivesi Sheep In Pregnancy And Postpartum, Sema Temi̇zer Ozan, Sema Yaralioğlu Gürgöze, Tülay İleri̇ Büyükoğlu, İhsan Hali̇feoğlu

Turkish Journal of Veterinary & Animal Sciences

Arginase (L-arginine amidino hydrolase E.C. 3.5.3.1) is an enzyme catalyzing the last stage of the Krebs-Henseleit cycle and, thus, converting arginine into urea and ornithine. Arginase also plays a part in protein and polyamine synthesis and is affected by hormones in terms of metabolic status. In the present study, erythrocyte, saliva, and serum arginase activity together with serum urea and estrogen levels were examined in Akkaraman and Ivesi sheep in pregnancy and postpartum. Comparisons were then made between the species. In pregnancy the urea level in saliva increased. However, the urea level in serum decreased. No clear difference was observed …

Determination Of Pregnancy, Embryonic-Fetal Mortality, Ovarium Functions And Uterus Diameter In Cows By Ultrasonography, Seli̇m Aslan, German Wesenauer

Turkish Journal of Veterinary & Animal Sciences

In this study 57 cows at the puerperal stage (between 15-45 days) and at early pregnancy period (between 8-58 days) were subjected to clinical and Ultrasonography check at 10-day intervals. On the 8 th day of post insemination (p.ins.) areas of nidation ranging from 0,5-1,5 cm were established by means of US (n=53) and on 40 animals (70,2%) the development of these areas were followed until the 58th day of pregnancy. In the other 13 cows (22,8%), begining from early embriyonic featal deaths (18) days and late embriyonic featal deaths (28 days) were established ultrasonographycally. 4 animals (7,0%) that no …

Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz

Obstetrics & Gynaecology Publications

In the present study the abundance of mRNAs for eukaryotic translation initiation factors eIF-1A (formerly known as eIF-4C), -2alpha, -4A, -4E, and -5 was examined in in vivo-derived mouse embryos throughout preimplantation development using a semiquantitative reverse transcription-polymerase chain reaction assay. Although the mRNA profile for each gene is unique, only mRNA for eIF-1A transiently increases during embryonic gene activation (EGA) at the 2-cell stage, and this was confirmed by an independent hybridization-based assay. In in vitro-developed bovine embryos, mRNA for eIF-1A was transiently detected at the 8-cell stage, when the major activation of the genome occurs in this species. …

Na/K-Atpase-Mediated 86rb+ Uptake And Asymmetrical Trophectoderm Localization Of Alpha1 And Alpha3 Na/K-Atpase Isoforms During Bovine Preattachment Development., D H Betts, L C Barcroft, A J Watson

Obstetrics & Gynaecology Publications

This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-subunit polypeptides were detected by immunofluorescence to encircle the entire cell margins of each blastomere of inseminated zygotes, cleavage stage embryos, and morulae. Immunofluorescent localization of alpha1-subunit polypeptide in bovine blastocysts revealed an alpha1 immunofluorescence signal confined to the basolateral membrane margins of the trophectoderm and encircling the cell periphery of each inner cell mass (ICM) cell. In contrast, alpha …

Experiencing Physical Violence During Pregnancy: Prevalence And Correlates, Vilma E. Cokkinides, Ann L. Coker

CRVAW Faculty Journal Articles

Violence during pregnancy directly impacts the mental and physical health of pregnant women. We assessed the prevalence and correlates of physical violence around the time of pregnancy in a representative sample of 6,718 women in South Carolina. Physical violence, defined as "being physically hurt by husband or partner" or "being involved in a physical fight" was reported by 10.9% of recently pregnant women. These were correlates of violence: experiencing increased numbers of stressful life events, being unmarried, having increased parity, being on Medicaid, and having an unwanted pregnancy. Screening to identify violence in pregnancy in health care settings is vital …

Exercise And Pregnancy: A Guide For Health Care Professionals, Stacy L. Ripplinger

Physical Therapy Scholarly Projects

Health and fitness has become nationwide trends resulting in people of all ages making wiser choices regarding nutrition and exercise. Pregnant women are also participating in this fitness revolution to help ease the discomforts of pregnancy, maintain fitness levels, and regain their shape after delivery. These women often turn to health care providers, including physical therapists, for guidance. The purpose of this independent study, accomplished by a review of the literature, is to provide physical therapists and general health care practitioners an overview of maternal body changes, nutritional requirements, and emotional changes that occur during pregnancy, and how these factors …

The Effects Of Shock Waves On The Rat Fetus, Orhan Yalçin, Lütfi Tahmaz, Zuhal Yumbul, İdris Bi̇lgi̇ç

Turkish Journal of Medical Sciences

There is an ongoing controversy regarding shock wave lithotripsy (SWL) contraindicated during pregnancy (1, 2). While the effects of shock waves on a variety of cells in both clinical and experimental studies are well documented, their effects on the fetus remain to be clarified. In this study, we investigated the effects of SWL on the rat fetus. On the 8th, 11th, 14th and 17th days of pregnancy, 100, 250 or 500 shock waves were produced with a Multimed 2001 ELMED Systems Lithotriptor. In ongoing pregnancy, the effects of SWL on fetuses ranged from mild in early pregnancy to moderate and …

Initial Multicenter Experience With Double Nucleoside Therapy For Human Immunodeficiency Virus Infection During Pregnancy., N S Silverman, D H Watts, J Hitti, D M Money, E Livingston, J Axelrod, J M Ernest, D Robbins, M M Divito

Department of Obstetrics and Gynecology Faculty Papers

OBJECTIVE: To study maternal and neonatal effects of combination nucleoside analog therapy administered to human immunodeficiency virus (HIV)-infected pregnant women for maternal indications.

METHODS: A multicenter, prospective observational study was undertaken at six perinatal centers in the United States and Canada that supported regional referral programs for the treatment of HIV-infected pregnant women. Demographic, laboratory, and pregnancy outcome data were collected for 39 women whose antiretroviral treatment regimens were expanded to include more than one nucleoside analog for maternal indications. The 40 newborns were monitored at pediatric referral centers through at least three months of age to ascertain their HIV …

Determinants Of Antepartum Human Immunodeficiency Virus Testing In A Non-Medicaid Obstetric Population., E Cardonick, S Daly, M Dooley, K Elles, N S Silverman

Department of Obstetrics and Gynecology Faculty Papers

OBJECTIVE: To determine voluntary human immunodeficiency virus (HIV) testing rates and factors influencing testing in a private obstetric practice.

METHODS: Antepartum patients were offered HIV testing after completing a self-assessment questionnaire. Perceived risks and demographics were correlated with testing rates.

RESULTS: Overall, 348/600 (58%) women consented to HIV testing. In a univariate analysis, patients with "any" perceived risk(s) were more likely to be tested. Single women and those with an at-risk partner(s) or a history of sexually transmitted disease (STD) were more likely to desire testing. These factors remained independently associated with voluntary testing in a multivariate regression model. No …

Proceedings Of The Women's Health: Women's Lives Conference: May 3rd 1998, Lynne Hunt (Ed.)

Research outputs pre 2011

The Women's Health: Women's Lives Conference was held on 3 May 1998, at Joondalup Campus, Edith Cowan University. The principal aim of the Conference was to celebrate 10 years of the teaching of Women's Health and Women's Studies at ECU, by drawing together past and present students, university staff and members of the Western Australian community interested in women's issues.

Exercise During Pregnancy, Sue Merkel

Physical Therapy Scholarly Projects

Physical fitness and sports participation has increased in popularity over the years. This raises concerns as to whether exercising while pregnant is beneficial or harmful to the mother and her unborn child. Research has determined the maternal and fetal responses during various forms of exercise. This research has been used to help establish guidelines and recommendations for pregnant women. However, there continues to be controversy as to the exercise guidelines pregnant females need to follow. Women experience physical changes throughout their pregnancy which can lead to low back pain, abdominal or pelvic floor dysfunctions. These complications can be prevented or …

Book Review Of Michael D. Green, Bendectin And Birth Defects: The Challenges Of Mass Toxic Substance Litigation, Stefan Oglevee

RISK: Health, Safety & Environment (1990-2002)

Review of Michael D. Green, Bendectin and Birth Defects: The Challenges of Mass Toxic Substances Litigation (University of Pennsylvania Press 1996). Acknowledgements, index, preface, selected bibliography. LC 95-42306; ISBN 0-8122-3257-7 [368 pp. $29.95 Cloth. 1300 Blockley Hall, 418 Service Drive, Philadelphia PA 19104-6097.]

Labour And Birth Information Needs Of First Time Mothers In Malawi And Satisfaction With Information Received, Address M. Malata

Theses: Doctorates and Masters

Giving labour and birth information to first time mothers is one of the challenges facing the midwives in Malawi. There are many underlying health and soeioeconomic problems facing the people of Malawi particularly mothers in the child bearing age because of inadequate human and material resources in the health sector. This thesis presents findings of a study carried out in Malawi to explore labour and birth information given to first time mothers at hospitals and in the communities. Maternal satisfaction with the information received was determined. In addition labour and birth information needs of first time mothers in Malawi were …

Fulminant Hepatic Failure In Pregnant Women: Acute Fatty Liver Or Acute Viral Hepatitis?, S S. Hamid, S M. Jafri, H Khan, H Shah, Z Abbas, H Fields

Section of Gastroenterology

Background: Hepatitis E virus, which is endemic in our region, can cause severe liver dysfunction in pregnant women and this can be clinically confused with acute fatty liver of pregnancy.

Methods: We studied the clinical and laboratory data as well as the maternal and fetal outcomes of 12 pregnant women presenting with fulminant hepatic failure in order to determine the etiology of the disease. The clinical diagnoses were subsequently correlated with serologic assays for acute HEV infection. All patients were severely ill with deep jaundice, grade 3-4 encephalopathy and abnormal prothrombin times.

Results: A clinical diagnosis of acute viral …

A Correlative Study Of An At-Risk Population And Low Birth Weight/Infant Mortality In A Northeast Oklahoma City Community, Estacia D. Thrower

McCabe Thesis Collection

Studies show that numerous and complex variables influence pregnancy outcomes and infant mortality rates. These variables include demographic, medical, physical, environmental, behavioral, and attitudinal factors and prenatal care. Improving the health of mothers and infants is a national challenge. In 1987, more than 3.8 million infants were born in the United States. Of these, 38,408 died before their first birthday. Although the infant mortality rate is at an all-time low, the pace of progress has slowed. According to Dr. Robert Deppen of the Oklahoma State Health Department, "In 1992, Oklahoma ranked 29th in the United States for infant mortality and …

Disruption Of The Cbfa2 Gene Causes Necrosis And Hemorrhaging In The Central Nervous System And Blocks Definitive Hematopoiesis., Qing Wang, Terryl Stacy, Michael M Binder, Miguel Marin-Padilla, Arlene H. Sharpe, Nancy A. Speck

Dartmouth Scholarship

The CBFA2 (AML1) gene encodes a DNA-binding subunit of the heterodimeric core-binding factor. The CBFA2 gene is disrupted by the (8;21), (3;21), and (12;21) chromosomal translocations associated with leukemias and myelodysplasias in humans. Mice lacking a CBF alpha 2 protein capable of binding DNA die between embryonic days 11.5 and 12.5 due to hemorrhaging in the central nervous system (CNS), at the nerve/CNS interfaces of cranial and spinal nerves, and in somitic/intersomitic regions along the presumptive spinal cord. Hemorrhaging is preceded by symmetric, bilateral necrosis in these regions. Definitive erythropoiesis and myelopoiesis do not occur in Cbfa2-deficient embryos, and disruption …

Analysis Of The Effects Of Overexpression Of Metallothionein-I In Transgenic Mice On The Reproductive Toxicology Of Cadmium., T Dalton, Kai Fu, G C Enders, R D Palmiter, G K Andrews

Journal Articles: Pathology and Microbiology

Exposure to low levels of cadmium reduces fertility. In male mice spermatogenesis is highly sensitive to cadmium, whereas in females the peri-implantation period of pregnancy is sensitive. To examine the potential roles of the cadmium-binding protein, metallothionein (MT), in the reproductive toxicology of cadmium, we examined a transgenic mouse strain that overexpresses metallothionein-I (MT-I). These mice had dramatically increased steady-state levels of MT-I mRNA and MT in the testes and in the female reproductive tract during the peri-implantation period of pregnancy, and this overexpression occurred in a cell-specific and temporally regulated manner similar to that of the endogenous MT-I gene. …

Survey Of Omega-3 Fatty Acids In Diets Of Midwest Low-Income Pregnant Women, Nancy M. Lewis, Anne C. Widga, Janet S. Buck, Andrea M. Frederick

Department of Nutrition and Health Sciences: Faculty Publications

The objectives of this project were to detennine the omega-3 fatty acid (ω-3 FA) consumption of low-income pregnant women, and to identify food sources of ω-3 FA in their diets. Thirty women provided three days of dietary intake that were analyzed using the Food Processor Plus computer program. Mean daily consumption of ω-3 FA was 1.060 ± 0.030 g/day. The major form of the ω-3 FA was α-linolenic acid (α-LNA; 93%), with less from eicosapentanoic acid (EPA; 2%), and docosahexanoic acid (DHA; 5%). Foods that provided the most α-LNA were fats, oils, salad dressings, and milk products. EPA …

Preimplantation Development Of In Vitro-Matured And In Vitro-Fertilized Ovine Zygotes: Comparison Between Coculture On Oviduct Epithelial Cell Monolayers And Culture Under Low Oxygen Atmosphere., A J Watson, P H Watson, D Warnes, S K Walker, D T Armstrong, R F Seamark

Obstetrics & Gynaecology Publications

The roles of medium composition, serum source, embryo coculture, and culture under low O2 conditions on the development of in vitro-matured and in vitro-fertilized (IVMF) ovine zygotes were investigated in three separate experiments. In the first experiment, the proportion of cocultured IVMF zygotes developing to the blastocyst stage was significantly higher (38.0% vs. 3.5%; p < 0.05) than that of non-cocultured zygotes treated within three embryo culture media (TCM-199 + 10% fetal bovine serum [FBS]; bicarbonate-buffered, glucose-free synthetic oviduct fluid medium [mod-SOFM] + 10% FBS; and bicarbonate-buffered BSA-free Tyrode's salt solution [mod-TALP] + 10% FBS) under a 5% CO2 atmosphere in air. In a second experiment, a significantly higher (p < 0.05) proportion of cocultured zygotes placed in TCM-199 medium survived to the blastocyst stage (37.4% blastocysts vs. 23.4% in mod-SOFM). No significant effect of serum (FBS vs. human serum [HS]) was observed on embryonic development, but coculture was confirmed to exert a significant influence on development to the blastocyst stage. In the final experiment, survival of the embryo under a reduced oxygen (5% CO2:5% O2:90% N2) atmosphere was investigated. In contrast to results in the initial experiments, embryonic survival was significantly higher (p < 0.05) in the non-cocultured treatment groups (21.9% blastocysts vs. 0.4% for cocultured zygotes). Serum source also had a significant (p < 0.05) influence upon the development of non-cocultured zygotes: 32.3% of zygotes cultured with HS progressed to the blastocyst stage vs. 11.5% of zygotes cultured in FBS-supplemented medium. These results have characterized two distinct culture environments, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro.