Medicine and Health Sciences Commons^™

Neutralization Of Il-33 Modifies The Type 2 And Type 3 Inflammatory Signature Of Viral Induced Asthma Exacerbation, Kristi J. Warren, Jill A. Poole, Jenea M. Sweeter, Jane M. Devasure, John D. Dickinson, R. Stokes Peebles, Todd A. Wyatt

Journal Articles: Environmental, Agricultural & Occupational Health

BACKGROUND: Respiratory viral infections are one of the leading causes of need for emergency care and hospitalizations in asthmatic individuals, and airway-secreted cytokines are released within hours of viral infection to initiate these exacerbations. IL-33, specifically, contributes to these allergic exacerbations by amplifying type 2 inflammation. We hypothesized that blocking IL-33 in RSV-induced exacerbation would significantly reduce allergic inflammation.

METHODS: Sensitized BALB/c mice were challenged with aerosolized ovalbumin (OVA) to establish allergic inflammation, followed by RSV-A2 infection to yield four treatment groups: saline only (Saline), RSV-infected alone (RSV), OVA alone (OVA), and OVA-treated with RSV infection (OVA-RSV). Lung outcomes included …

Inhibitory Molecules Pd-1, Cd73 And Cd39 Are Expressed By Cd8+ T Cells In A Tissue-Dependent Manner And Can Inhibit T Cell Responses To Stimulation, Corinne J. Smith, Christopher M. Snyder

Department of Microbiology and Immunology Faculty Papers

The salivary gland is an important tissue for persistence and transmission of multiple viruses. Previous work showed that salivary gland tissue-resident CD8+ T cells elicited by viruses were poorly functional ex vivo. Using a model of persistent murine cytomegalovirus (MCMV) infection, we now show that CD8+ T cells in the salivary gland and other non-lymphoid tissues of mice express multiple molecules associated with T cell exhaustion including PD-1, CD73 and CD39. Strikingly however, these molecules were expressed independently of virus or antigen. Rather, PD-1-expressing T cells remained PD-1+ after migration into tissues regardless of infection, while CD73 was activated on …

Rabies Postexposure Prophylaxis In The United States: Opportunities To Improve Access, Coordination, And Delivery, Gavin T. Howington, Huy-Binh Nguyen, P. Brandon Bookstaver, Peter Akpunonu, Joshua T. Swan

Pharmacy Practice and Science Faculty Publications

No abstract provided.

Epiglottic Squamous Cell Carcinoma., John Carter, Jessica Ball, George Mckean, Samir Patel

Oncology Articles

No abstract provided.

A Novel Behavioral Model In Initiation And Sustenance Of Toothbrushing Behavior Among Dental And Medical Students In India: An Exploratory Analysis, Dhiraj Panjwani, Mithun Pai, Shweta Yellapurkar, Aayush Poddar, Gururaghavendran Rajesh, Manoj Sharma

Environmental & Global Health Faculty Research

Purpose: Toothbrushing holds key importance in practical, competent, and efficacious design to limit the formation of dental plaque on the dental tissues, in turn, leading to a decrease in dental diseases. The recent addition to this growing knowledge in health behavior research is the multi-Theory model (MTM) which addresses two crucial modules of health behavior: initiation and sustenance. In spite of reinforcing the habit of brushing twice daily, a lack of sustenance is found among young adults; hence, the objectives were to explore the substratal structure of MTM and to test the fit of MTM questionnaire in dental and medical …

Gut Integrity And Duodenal Enteropathogen Burden In Undernourished Children With Environmental Enteric Dysfunction, Zehra Jamil, Najeeha Talat Iqbal, Romana Idress, Zubair Ahmad, Kamran Sadiq, Junaid Iqbal, Sana Syed, Aneeta Hotwani, Furqan Kabir, Kumail Ahmed, Sheraz Ahmed, Fayyaz Umrani, Fatima Aziz, Adil Kalam, Syed Asad Ali

Department of Biological & Biomedical Sciences

Environmental enteric dysfunction (EED) is a subclinical condition of intestinal inflammation, barrier dysfunction and malabsorption associated with growth faltering in children living in poverty. This study explores association of altered duodenal permeability (lactulose, rhamnose and their ratio) with higher burden of enteropathogen in the duodenal aspirate, altered histopathological findings and higher morbidity (diarrhea) that is collectively associated with linear growth faltering in children living in EED endemic setting. In a longitudinal birth cohort, 51 controls (WHZ > 0, HAZ > -1.0) and 63 cases (WHZ< -2.0, refractory to nutritional intervention) were recruited. Anthropometry and morbidity were recorded on monthly bases up to 24 months of age. Dual sugar assay of urine collected after oral administration of lactulose and rhamnose was assessed in 96 children from both the groups. Duodenal histopathology (n = 63) and enteropathogen analysis of aspirate via Taqman array card (n = 60) was assessed in only cases. Giardia was the most frequent pathogen and was associated with raised L:R ratio (p = 0.068). Gastric microscopy was more sensitive than duodenal aspirate in H. pylori detection. Microscopically confirmed H. pylori negatively correlated with HAZ at 24 months (r = -0.313, p = 0.013). Regarding histopathological parameters, goblet cell reduction significantly correlated with decline in dual sugar excretion (p< 0.05). Between cases and controls, there were no significant differences in the median (25th, 75th percentile) of urinary concentrations (μg/ml) of lactulose [27.0 (11.50, 59.50) for cases vs. 38.0 (12.0, 61.0) for controls], rhamnose [66.0 (28.0, 178.0) vs. 86.5 (29.5, 190.5)] and L:R ratio [0.47 (0.24, 0.90) vs. 0.51 (0.31, 0.71)] respectively. In multivariable regression model, 31% of variability in HAZ at 24 months of age among cases and controls was explained by final model including dual sugars. In conclusion, enteropathogen burden is associated with altered histopathological features and intestinal permeability. In cases and controls living in settings of endemic enteropathy, intestinal permeability test may predict linear growth. However, for adoption as a screening tool for EED, further validation is required due to its complex intestinal pathophysiology.

Cyclic Amp Is Dispensable For Allorecognition In Dictyostelium Cells Overexpressing Pka-C, Shigenori Hirose, Mariko Katoh-Kurasawa, Gad Shaulsky

Faculty, Staff and Students Publications

Allorecognition and tissue formation are interconnected processes that require signaling between matching pairs of the polymorphic transmembrane proteins TgrB1 and TgrC1 in Dictyostelium. Extracellular and intracellular cAMP signaling are essential to many developmental processes. The three adenylate cyclase genes, acaA, acrA and acgA are required for aggregation, culmination and spore dormancy, respectively, and some of their functions can be suppressed by activation of the cAMP-dependent protein kinase PKA. Previous studies have suggested that cAMP signaling might be dispensable for allorecognition and tissue formation, while others have argued that it is essential throughout development. Here, we show that allorecognition and tissue …

An Assessment Of The Social Determinants Of Health In An Urban Emergency Department, Edgardo Ordonez, Katherine Dowdell, Natasha M Navejar, Deepa Dongarwar, Aya Itani, Hamisu M Salihu

Faculty, Staff and Students Publications

INTRODUCTION: Social determinants of health (SDOH) have significant impacts on patients who seek care in the emergency department (ED). We administered a social needs screening tool and needs assessment survey to assess SDOH and evaluate for trends in the population of patients visiting our ED.

METHODS: A survey was distributed via convenience sampling to adult ED patients to capture self-reported demographic information and data about social needs. We categorized the questions related to SDOH based on the International Classification of Diseases, Tenth Revision, Clinical Modification coding format and created a composite variable called "SDOH Strata" based on the SDOH Index …

Discovery Of A New Cdk4/6 And Pi3k/Akt Multiple Kinase Inhibitor Aminoquinol For The Treatment Of Hepatocellular Carcinoma., Zhong-Kun Xia, Wei Wang, Jian-Ge Qiu, Xi-Nan Shi, Hong-Jian Li, Rong Chen, Kun-Bin Ke, Chao Dong, Ying Zhu, Shi-Guo Wu, Rong-Ping Zhang, Zhuo-Ran Meng, Hui Zhao, Peng Gu, Kwong-Sak Leung, Man-Hon Wong, Xiao-Dong Liu, Feng-Mei Zhou, Jian-Ying Zhang, Ya-Ting Yao, Si-Jia Wang, Chun-Yang Zhang, Yan-Ru Qin, Marie Chia-Mi Lin, Bing-Hua Jiang

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Background: Hepatocellular carcinoma (HCC) is a lethal malignancy lacking effective treatment. The Cyclin-dependent kinases 4/6 (CDK4/6) and PI3K/AKT signal pathways play pivotal roles in carcinogenesis and are promising therapeutic targets for HCC. Here we identified a new CDK4/6 and PI3K/AKT multi-kinase inhibitor for the treatment of HCC.

Methods: Using a repurposing and ensemble docking methodology, we screened a library of worldwide approved drugs to identify candidate CDK4/6 inhibitors. By MTT, apoptosis, and flow cytometry analysis, we investigated the effects of candidate drug in reducing cell-viability,inducing apoptosis, and causing cell-cycle arrest. The drug combination and thermal proteomic profiling (TPP) method were …

Medical Nutrition Therapy For Diabetes, Cheryl Marco, Rd, Cde

Department of Family & Community Medicine Presentations and Grand Rounds

No abstract provided.

Using The Multi-Theory Model (Mtm) Of Health Behavior Change To Explain The Correlates Of Mammography Screening Among Asian American Women, Manoj Sharma, Chia-Liang Dai, Kavita Batra, Ching-Chen Chen, Jennifer R. Pharr, Courtney Coughenour, Asma Awan, Hannah Catalano

Environmental & Global Health Faculty Research

Globally, breast cancer is the most common malignancy affecting women. The incidence of breast cancer has been growing among Asian American women. Mammography is a screening procedure that provides early diagnosis for the timely treatment to reduce premature mortality due to breast cancer. However, there are no national data available that summarize the rates of mammography screening among Asian American women. Some small-scale studies have reported low rates of mammography uptake among Asian American women. This cross-sectional study utilized the fourth-generation, multi-theory model (MTM) of health behavior change to explain the correlates of mammography screening among Asian American women between …

A Retrospective Review On Antibiotic Use In Acute Watery Diarrhea In Children In A Tertiary Care Hospital Of Karachi, Pakistan, Sonia Qureshi, Shahzadi Resham, Mariam Hashmi, Abdullah B. Naveed, Zoya Haq, Syed Asad Ali

Department of Paediatrics and Child Health

Introduction: Responsible for at least one in nine pediatric deaths, diarrheal diseases are the leading, global cause of death. Further abetted by improper antibiotic use in a hospital setting, children with acute watery diarrhea can see prolonged hospital stays, and unwanted adverse effects such as antibiotic resistance. Hence, this study is aimed to identify the association between antibiotic usage for the treatment of acute watery diarrhea in children, and the impact this line of management has on the duration of their hospital stay.
Methods: A retrospective review was conducted at the department of Pediatric of Aga Khan University Hospital (AKUH) …

Blood's Concentration Of Lead And Arsenic Associated With Anemia In Peruvian Children, Ana Maria Linares, Jason M. Unrine, Amanda Thaxton Wigging, Juan C. Tantalean, Vlad C. Radulescu

Nursing Faculty Publications

This exploratory, descriptive cohort study (N = 60) determined lead (Pb) and arsenic (As) blood concentrations in Peruvian children and their association with hematological parameters of iron-deficient anemia (IDA) and anthropometric measurement. The mean age of children was 10.8 months (SD = 4.7) and ranged from 3 to 24 months old. Anemia (Hb levels below 10.5 g/dL) was found in 20% of this cohort. Additionally, microcytosis (MCV < 70 fL) was present in 54%, and hypochromia (MCH < 23 pg) in 42% of the group of children. Chi-square analysis showed that 88% of the children with anemia also had microcytosis and hypochromia (p < 0.001). Pb and As were detected in 100% of the infants’ blood samples, and the concentrations were significantly higher in older infants than in younger ones. Pb and As were not associated with the sex, anthropomorphic parameters, or infant hemogram changes. Infants who received iron supplementation were 87% less likely to have low Hb compared with those who did not (OR = 0.13, 95% CI = 0.02–0.88, p = 0.04). Herbal tea intake was significantly associated with microcytosis and hypochromia. Our finding uncovered that hematological parameters for anemia are modified in Peruvian children with high levels of microcytosis …

Significant Regional Differences In Antibiotic Use Across 576 Us Hospitals And 11 701 326 Adult Admissions, 2016-2017, Katherine E Goodman, Sara E Cosgrove, Lisa Pineles, Laurence S Magder, Deverick J Anderson, Elizabeth Dodds Ashley, Ronald E Polk, Hude Quan, William E Trick, Keith F Woeltje, Surbhi Leekha, Anthony D Harris

2020-Current year OA Pubs

BACKGROUND: Quantifying the amount and diversity of antibiotic use in United States hospitals assists antibiotic stewardship efforts but is hampered by limited national surveillance. Our study aimed to address this knowledge gap by examining adult antibiotic use across 576 hospitals and nearly 12 million encounters in 2016-2017.

METHODS: We conducted a retrospective study of patients aged ≥ 18 years discharged from hospitals in the Premier Healthcare Database between 1 January 2016 and 31 December 2017. Using daily antibiotic charge data, we mapped antibiotics to mutually exclusive classes and to spectrum of activity categories. We evaluated relationships between facility and case-mix …

Disability Injustice: A Latino’S Creative Autoethnographic Testimonio On The Organizational Culture Of Higher Education, Leonel A. Diaz Jr.

Organization, Information and Learning Sciences ETDs

Using creative autoethnographic testimonio (CAT), a story is told about the injustices within the learning environment and work environment of higher education toward a person with disabilities: sleep apnea, learning disabilities, negative mental health. The author explores the health difficulties of addressing sleep deprivation while attending graduate school and working full-time as a professional. With sleep apnea impacting his health, his mental health declines. As his health declines, there is an increase in discrimination, hostility, oppression, bullying, and toxic masculinity. Initially, the medical system dismisses his declining health and refuses to look further into it. Once he receives medical care …

Main Concept, Sequencing, And Story Grammar (Mssg) Analyses Of The Cinderella Story In Latent Aphasia, Janet B. Adams

Speech and Hearing Sciences ETDs

Commonly used standardized tests, like the Western Aphasia Battery-Revised (WAB-R), are not sensitive to higher level discourse deficits, leading to certain individuals not meeting diagnostic criteria for aphasia. Consequently, individuals with aphasia are excluded from receiving potentially beneficial services and/or from being included in research. In a large sample of persons with stroke-induced aphasia, this study analyzed discourse samples using Main Concept, Sequencing, and Story Grammar (MSSG) Analyses to examine macrostructural discourse characteristics of persons with latent aphasia (PWLAs) compared to persons with no underlying brain injury (PNBIs) and persons classified as having anomic aphasia (PWAAs) by the WAB-R. A …

Understanding Covid-19 Dynamics And The Effects Of Interventions In The Philippines: A Mathematical Modelling Study, Jamie M. Caldwell, Elvira P. De Lara-Tuprio, Timothy Robin Y. Teng, Ma. Regina Justina E. Estuar, Raymond Francis R. Sarmiento, Milinda Abayawardana B. Eng, Robert Neil F. Leong, Richard T. Gray, James G. Wood, Linh-Vi Le, Emma S. Mcbryde, Romain Ragonnet, James M. Trauer

Mathematics Faculty Publications

Background

COVID-19 initially caused less severe outbreaks in many low- and middle-income countries (LMIC) compared with many high-income countries; possibly because of differing demographics; socioeconomics; surveillance; and policy responses. Here; we investigate the role of multiple factors on COVID-19 dynamics in the Philippines; a LMIC that has had a relatively severe COVID-19 outbreak.

Methods

We applied an age-structured compartmental model that incorporated time-varying mobility; testing; and personal protective behaviors (through a “Minimum Health Standards” policy; MHS) to represent the first wave of the Philippines COVID-19 epidemic nationally and for three highly affected regions (Calabarzon; Central Visayas; and the National Capital …

Nih Portfolio Of Unintentional Injury Research Among Racial And Ethnic Minority Children: Current Landscape And Future Opportunities, Maria Isabel Roldos, Tilda Farhat, Thomas Moehlman

Publications and Research

Unintentional injuries (UIs) caused by accidental suffocation, burns, drowning, falls, poisoning, and motor vehicle accidents are the leading causes of morbidity and mortality among children (Dellinger and Gilchrist. Am J Lifestyle Med; 2017). Notable racial and ethnic disparities exist in accidental suffocation among infants and in motor vehicle injuries (MVI) among youth. The purpose of this study is to examine the National Institutes of Health's funded research projects addressing UIs, using a socioecological framework, and to determine whether funded projects align with key priorities for unintentional injuries among racial and ethnic minorities as identified by the research community. Between 2011 …

Increasing The Use Of Permethrin To Prevent Zika Infections Among University Students, Islam Khalil, Laura Yut, Kulsum Agha, Leticia Furlani Bodanese, Stephanie Barajas, Jhonsley Frederic, Andrea Catalina Silva, William W. Darrow

Florida Public Health Review

Background. In summer 2016, Miami-Dade County, Florida, declared a public health emergency when reports of locally acquired Zika infections were confirmed. Officials at a large public university in the county warned students of the risks and advocated the use of repellents and permethrin to prevent mosquito bites. A subsequent study showed few students (2.9%) sprayed their clothes with permethrin. Purpose. In the absence of a safe and effective vaccine, a team of Master of Public Health students sought to determine if a brief educational intervention might increase permethrin use. Methods. Students living in six dormitories were chosen as the …

Robotic Assistance In Unicompartmental Knee Arthroplasty Results In Superior Early Functional Recovery And Is More Likely To Meet Patient Expectations., Meredith P. Crizer, Amer Haffar, Andrew Battenberg, Mikayla Mcgrath, Ryan Sutton, Jess H Lonner

Rothman Institute Papers

Robotic technology has reduced the errors of implant alignment in unicompartmental knee arthroplasty (UKA), but its impact on functional recovery after UKA is poorly defined. The purpose of this study was to compare early functional recovery, pain levels, and satisfaction in UKA performed with either robotic assistance or conventional methods. A retrospective analysis was performed on 89 matched consecutive patients who underwent outpatient UKA by a single physician using either conventional instruments (n = 39) or robotic methods (n = 50), with otherwise identical perioperative protocols. Outcomes studied included Lower Extremity Functional Score (LEFS), new Knee Society Score …

Capacity Building For A New Multicenter Network Within The Echo Idea States Pediatric Clinical Trials Network., Robert D Annett, Scott Bickel, John C Carlson, Kelly Cowan, Sara Cox, Mark J Fisher, J Dean Jarvis, Alberta S Kong, Jessica S Kosut, Kurtis R Kulbeth, Abbot Laptook, Pearl A Mcelfish, Mary M Mcnally, Lee Pachter, Do, Barbara A Pahud, Lee A Pyles, Jennifer Shaw, Kari Simonsen, Jessica Snowden, Christine B Turley, Andrew M Atz

Department of Medicine Faculty Papers

Introduction: Research capacity building is a critical component of professional development for pediatrician scientists, yet this process has been elusive in the literature. The ECHO IDeA States Pediatric Clinical Trials Network (ISPCTN) seeks to implement pediatric trials across medically underserved and rural populations. A key component of achieving this objective is building pediatric research capacity, including enhancement of infrastructure and faculty development. This article presents findings from a site assessment inventory completed during the initial year of the ISPCTN.

Methods: An assessment inventory was developed for surveying ISPCTN sites. The inventory captured site-level activities designed to increase clinical trial research …

Human Apobec3 Variations And Viral Infection, Shiva Sadeghpour, Saeideh Khodaee, Mostafa Rahnama, Hamzeh Rahimi, Diako Ebrahimi

Plant Pathology Faculty Publications

Human APOBEC3 (apolipoprotein B mRNA-editing catalytic polypeptide-like 3) enzymes are capable of inhibiting a wide range of endogenous and exogenous viruses using deaminase and deaminase-independent mechanisms. These enzymes are essential components of our innate immune system, as evidenced by (a) their strong positive selection and expansion in primates, (b) the evolution of viral counter-defense mechanisms, such as proteasomal degradation mediated by HIV Vif, and (c) hypermutation and inactivation of a large number of integrated HIV-1 proviruses. Numerous APOBEC3 single nucleotide polymorphisms, haplotypes, and splice variants have been identified in humans. Several of these variants have been reported to be associated …

Rapid Development Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Purulent Pericarditis In The Setting Of Endocarditis, Samiullah Arshad, Naoki Misumida

Internal Medicine Faculty Publications

Methicillin-resistant Staphylococcus aureus (MRSA) purulent pericarditis is a rare but potentially fatal complication of MRSA bacteremia. We describe a case of a 27-year-old patient with active intravenous drug use, who presented with fever, chills, and dyspnea and was found to have tricuspid valve endocarditis. Echocardiogram on admission showed no pericardial effusion. The patient became hypotensive, with worsening dyspnea, in the following 3 days. A computed tomography scan of the chest was repeated and showed a large pericardial effusion. The patient underwent pericardiocentesis and pericardial drain placement. Antibiotics were continued, with resolution of effusion. Early pericardiocentesis of a large purulent pericardial …

Predictors Of Permanent Pacemaker Implantation In Patients Undergoing Transcatheter Aortic Valve Replacement - A Systematic Review And Meta-Analysis., Waqas Ullah, Salman Zahid, Syeda Ramsha Zaidi, Deepika Sarvepalli, Shujaul Haq, Sohaib Roomi, Maryam Mukhtar, Muhammad Atif Khan, Smitha Narayana Gowda, Nicholas Ruggiero, Alec Vishnevsky, David L. Fischman

Abington Jefferson Health Papers

Background As transcatheter aortic valve replacement (TAVR) technology expands to healthy and lower-risk populations, the burden and predictors of procedure-related complications including the need for permanent pacemaker (PPM) implantation needs to be identified.

Methods and Results Digital databases were systematically searched to identify studies reporting the incidence of PPM implantation after TAVR. A random- and fixed-effects model was used to calculate unadjusted odds ratios (OR) for all predictors. A total of 78 studies, recruiting 31 261 patients were included in the final analysis. Overall, 6212 patients required a PPM, with a mean of 18.9% PPM per study and net rate …

Acute Lung Injury In Cholinergic-Deficient Mice Supports Anti-Inflammatory Role Of Α7 Nicotinic Acetylcholine Receptor, Nathalia M. Pinheiro, Rosana Banzato, Lolanda Tiberio, Marco A. M. Prado, Vivian F. Prado, Ayman K. Hamouda, Carla M. Prado

Pharmacy Faculty Publications and Presentations

(1) Background: The lung cholinergic pathway is important for controlling pulmonary inflammation in acute lung injury, a condition that is characterized by a sudden onset and intense inflammation. This study investigated changes in the expression levels of nicotinic and muscarinic acetylcholine receptors (nAChR and mAChR) in the lung during acute lung injury. (2) Methods: acute lung injury (ALI) was induced in wild-type and cholinergic-deficient (VAChT-KDHOM) mice using intratracheal lipopolysaccharide (LPS) instillation with or without concurrent treatment with nicotinic ligands. Bronchoalveolar lavage fluid was collected to evaluate markers of inflammation, and then the lung was removed and processed for isolation of …

Use Of Standardized Test Results By Faculty And Students For Advanced Practice Nurse Certification Success, Dinorah Martinez-Anderson

Nursing Theses and Dissertations

The utilization of standardized testing as a recognized educational strategy to measure pre-professional readiness in Advanced Practice Nursing (APN) graduate programs has increased. Research supports such use for predicting licensure success for undergraduate education, however when related to the use in graduate nursing education, it is limited. Teaching and learning practices of APN faculty and students have not been comprehensively explored. This study examined the use of standardized testing results by faculty and students in APN graduate education that contributes to student national certification success. The Reconceptualization of Vygotsky’s Theory of Zone of Proximal Development (RVZPD) underpins the study. An …

Severe Covid-19 Is Characterized By An Impaired Type I Interferon Response And Elevated Levels Of Arginase Producing Granulocytic Myeloid Derived Suppressor Cells, Matthew J. Dean, Juan B. Ochoa, Maria Dulfary Sanchez-Pino, Jovanny Zabaleta, Jone Garai, Luis Del Valle, Dorota Wyczechowska, Lyndsey Buckner Baiamonte, Phaethon Philbrook, Rinku Majumder, Richard S. Vander Heide, Logan Dunkenberger, Ramesh Puttalingaiah Thylur, Bobby Nossaman, W. Mark Roberts, Andrew G. Chapple, Jiande Wu, Chindo Hicks, Jack Collins, Brian Luke

School of Medicine Faculty Publications

COVID-19 ranges from asymptomatic in 35% of cases to severe in 20% of patients. Differences in the type and degree of inflammation appear to determine the severity of the disease. Recent reports show an increase in circulating monocytic-myeloid-derived suppressor cells (M-MDSC) in severe COVID 19 that deplete arginine but are not associated with respiratory complications. Our data shows that differences in the type, function and transcriptome of granulocytic-MDSC (G-MDSC) may in part explain the severity COVID-19, in particular the association with pulmonary complications. Large infiltrates by Arginase 1+ G-MDSC (Arg+G-MDSC), expressing NOX-1 and NOX-2 (important for production of reactive oxygen …

The Role Of Interspecies Recombination In The Evolution Of Antibiotic-Resistant Pneumococci, Joshua C. D'Aeth, Mark Pg Van Der Linden, Lesley Mcgee, Herminia De Lencastre, Paul Turner, Jae-Hoon Song, Stephanie W. Lo, Rebecca A. Gladstone, Gps Consortium, Sadia Shakoor

Department of Pathology and Laboratory Medicine

Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1-type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking …

Longitudinal Association Between Parental-To-Child-Maltreatment And Self-Reported Generalized Anxiety Disorder Symptoms In Pakistani Adolescents, Maryam Pyar Ali Lakhdir, Ghazal Peerwani, Salman Muhammad Soomar, Apsara Ali, Salima Farooq, Naureen Akber Ali, Asif Khaliq, Muhammad Masood Kadir, Syed Iqbal Azam

Community Health Sciences

Background: Parent-to-child maltreatment is considered one of the risk factors for Generalized Anxiety Disorder (GAD) symptoms, but this hypothesis has not been adequately tested in Pakistani settings.
Aim: This study aimed to examine the association between parent-to-child maltreatment and the risk of developing GAD symptoms among adolescents.
Methods: The association of none to rare, occasionally, and frequently parent-to-child maltreatment with the incidence of GAD symptoms was investigated in a sample of 800 adolescents aged 11-17 years who were followed for two years. Parent-to-child maltreatment was assessed using ICAST-C (International child abuse screening tool). GAD Symptoms were determined by SCARED (Screen …

Factors Influencing Rural Women’S Physical Activity Behaviors: A Social Ecological Perspective, Karen Lynn Webb

Nursing ETDs

ABSTRACT

Chronic disease and poor mental health are disproportionally impacting adults living in rural America. Regular physical activity has been shown to promote overall well-being and prevent many chronic diseases. Yet, research shows that many rural adults do not engage in enough physical activity to meet the recommended levels put forth by the federal government. The purpose of this descriptive qualitative study was to explore and describe the experiences of physical activity as perceived by rural women in their own words. Data were collected through semi-structured individual interviews of 20 women aged 25 to 34 living in McDowell County, West …