Medicine and Health Sciences Commons^™

By Utilizing Technology Can Nursing Students Gain More Confidence And Decrease Anxiety When Communicating With Chronically Ill Patients About Their Sexual Relationship?, Sheri Rickman Patrick, Alice Butzlaff

Faculty Research, Scholarly, and Creative Activity

Introduction
Effective communication is an essential part of nursing care. Nurses need to effectively communicate with patients, families, providers and staff. The purpose of this study was to show how the use of technology (i.e., video conferencing on an electronic device) could assist nursing students to gain more confidence and reduce anxiety when discussing difficult topics such as sexual intimacy.
Methods
Pre-licensure nursing students were recruited to participate as part of regular class activities. A pre- and post-survey asking about their confidence and anxiety in communicating with patients was completed; then subjects were randomly placed into groups of 4 to …

Molecular Characterization Of Trypanosoma Vivax In Tsetse Flies Confirms The Presence Of The Virulent Tvv4 Genotype In Kenya: Potential Implications For The Control Of Trypanosomiasis In Shimba Hills, Faith I. Ebhodaghe, Armanda D.S. Bastos, Kevin O. Kidambasi, Shewit Kalayou, Daniel K. Masiga, Michael N. Okal

All Peer-Reviewed Publications

Trypanosoma vivax is a vector-borne protozoan parasite of livestock endemic to Africa and South America. To date, fifteen genotypes of the parasite have been described in vertebrate and insect hosts in East Africa. However, information regarding T. vivax diversity remains limited in many endemic countries in the sub-region, including Kenya. Such information could deepen insight into the local epidemiology of animal trypanosomiasis in Shimba Hills, a wildlife area in southeast Kenya where T. vivax is endemic and infects livestock. We employed two-gene conventional-PCR-sequencing and phylogenetic analysis to characterize T. vivax genotypes in tsetse flies collected between November 2018 and September …

Effect Of Finishing And Polishing Techniques On The Fit Accuracy And Dimensions Of Conventional And Cad-Cam Removable Partial Denture Frameworks, Majed Altoman

Loma Linda University Electronic Theses, Dissertations & Projects

Rapid advancements in computer-aided design and computer-aided manufacturing (CAD-CAM) in removable dental prosthodontics have offered new modalities for fabrication of removable partial denture frameworks. The standard tessellation language (STL) files of the scans are used to digitally design the removable partial denture (RPD) framework, which are then printed in cobalt-chromium (Co-Cr) alloy and finished and polished according to manufacturers’ recommendations. There are few studies compare the fit accuracy of the cast DPD framework versus the CAD-CAM RPD frameworks which found that CAD-RP frameworks exhibited the highest discrepancies, using different methods of evaluation. Good fit between the denture base and the …

Accuracy Of Digital Indirect Bonding Technique Utilizing 3d-Printed Trays, Phillip Ha

Loma Linda University Electronic Theses, Dissertations & Projects

Purpose: This study investigated the bracket placement accuracy of a digital indirect bonding (dIDB) technique utilizing 3D-printed trays and the effects of dental crowding and tooth type on bracket placement accuracy.

Material and Methods: Thirty digital mandibular models of various amounts of arch length discrepancy (ALD) were categorized into 3 groups according to the amount of ALD. Digital bracket placement was performed from second molar to second molar using Indirect Bonding Studio (3ShapeTM, Copenhagen, Denmark) and this virtual model was used as the control. Indirect bonding trays were designed using Appliance Designer (3ShapeTM) and 3D-printed with the SprintRayTM …

Global Trends In Cancer Nanotechnology: A Qualitative Scientific Mapping Using Content-Based And Bibliometric Features For Machine Learning Text Classification, Nuwan Gedara, Xuan Xu, Robert Delong, Santosh Aryal, Majid Jaberi-Douraki

Pharmacy Faculty Publications and Presentations

This study presents a new way to investigate comprehensive trends in cancer nanotechnology research in different countries, institutions, and journals providing critical insights to prevention, diagnosis, and therapy. This paper applied the qualitative method of bibliometric analysis on cancer nanotechnology using the PubMed database during the years 2000-2021. Inspired by hybrid medical models and content-based and bibliometric features for machine learning models, our results show cancer nanotechnology studies have expanded exponentially since 2010. The highest production of articles in cancer nanotechnology is mainly from US institutions, with several countries, notably the USA, China, the UK, India, and Iran as concentrated …

Addressing And Inspiring Vaccine Confidence In Black, Indigenous, And People Of Color During The Coronavirus Disease 2019 Pandemic, Jasmine R Marcelin, Talia H Swartz, Fidelia Bernice, Vladimir Berthaud, Robbie Christian, Christopher Da Costa, Nada Fadul, Michelle Floris-Moore, Matifadza Hlatshwayo, Patrik Johansson, Ravina Kullar, Kimberly Manning, Edo-Abasi U. Mcgee, Christopher Medlin, Damani A Piggott, Uzma Syed, Jessica Snowden, Tina Tan, Jacinda C Abdul-Mutakabbir

PCOM Scholarly Works

During the coronavirus disease 2019 (COVID-19) pandemic, we have witnessed profound health inequities suffered by Black, Indigenous, and People of Color (BIPOC). These manifested as differential access to testing early in the pandemic, rates of severe disease and death 2-3 times higher than white Americans, and, now, significantly lower vaccine uptake compared with their share of the population affected by COVID-19. This article explores the impact of these COVID-19 inequities (and the underlying cause, structural racism) on vaccine acceptance in BIPOC populations, ways to establish trustworthiness of healthcare institutions, increase vaccine access for BIPOC communities, and inspire confidence in COVID-19 …

Investigating The Role Of Functional Polymorphism Of Maternal And Neonatal Vitamin D Binding Protein In The Context Of 25‐Hydroxyvitamin D Cutoffs As Determinants Of Maternal‐Neonatal Vitamin D Status Profiles In A Sunny Mediterranean Region, Spyridon N. Karras, Erdinç Dursun, Merve Alaylıoğlu, Duygu Gezen‐Ak, Cedric Annweiler, Fatme Al Anouti, Hana M.A. Fakhoury, Alkiviadis Bais, Dimitrios Kiortsis

All Works

Recent results indicate that dysregulation of vitamin D‐binding protein (VDBP) could be involved in the development of hypovitaminosis D, and it comprises a risk factor for adverse fetal, maternal and neonatal outcomes. Until recently, there was a paucity of results regarding the effect of maternal and neonatal VDBP polymorphisms on vitamin D status during pregnancy in the Mediterranean region, with a high prevalence of hypovitaminosis D. We aimed to evaluate the combined effect of maternal and neonatal VDBP polymorphisms and different maternal and neonatal 25‐hy-droxyvitamin D (25(OH)D) cut‐offs on maternal and neonatal vitamin D profile. Blood samples were obtained from …

Automatic Cerebrovascular Segmentation Methods - A Review, Fatma Taher, Neema Prakash

All Works

Cerebrovascular diseases are one of the serious causes for the increase in mortality rate in the world which affect the blood vessels and blood supply to the brain. In order, diagnose and study the abnormalities in the cerebrovascular system, accurate segmentation methods can be used. The shape, direction and distribution of blood vessels can be studied using automatic segmentation. This will help the doctors to envisage the cerebrovascular system. Due to the complex shape and topology, automatic segmentation is still a challenge to the clinicians. In this paper, some of the latest approaches used for segmentation of magnetic resonance angiography …

Coronavirus Disease 2019 (Covid-19): Multisystem Review Of Pathophysiology, Tanveer Mir, Talal Almas, Jasmeet Kaur, Mohammed Faisaluddin, David Song, Waqas Ullah, Sahil Mamtani, Hiba Rauf, Sunita Yadav, Sharaad Latchana, Nara Miriam Michaelson, Michael Connerney, Yasar Sattar

Division of Internal Medicine Faculty Papers & Presentations

Abstract Coronavirus disease-19 (COVID-19) pandemic is associated with high morbidity and mortality. COVID-19, which is caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2), affects multiple organ systems through a myriad of mechanisms. Afflicted patients present with a vast constellation of symptoms, from asymptomatic disease to life-threatening complications. The most common manifestations pertain to mild pulmonary symptoms, which can progress to respiratory distress syndrome and venous thromboembolism. However, in patients with renal failure, life-threatening cardiac abnormalities can ensue. Various mechanisms such as viral entry through Angiotensin receptor (ACE) affecting multiple organs and thus releasing pro-inflammatory markers have been postulated. …

Rabbit Control: Rhdv1 K5, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity factsheets

The national release of the Korean strain of rabbit haemorrhagic disease virus, RHDV1 K5, took place during the first week of March 2017. It was the first time in 20 years a new rabbit biocontrol agent had been released into Australia, and was part of a 20-year national biocontrol plan for rabbits. More than 600 release sites were selected, including 110 sites in Western Australia (WA).

RHDV1 K5 is a naturally occurring Korean variant of the rabbit haemorrhagic disease virus (RHDV). Its release was expected to boost management and help slow the increase in rabbit numbers. RHDV1 K5 does not …

In Vitro Biomechanical Comparison Of Clamped Suture Knot Techniques As A Model Of Extra-Capsular Stabilization Of Canine Cruciate Ligament Repair, Jung-Jin Lee, Mu-Young Kim, Hun-Young Yoon

The Thai Journal of Veterinary Medicine

Six clamped suture knot techniques were compared to identify the effects on the biomechanic properties used as a model of extra-capsular stabilization. Six clamped suture knot techniques included square knot formed by clamping the first throw with toothed mosquito forceps (SQ-TM), non-toothed mosquito forceps (SQ-NTM), or needle holder (SQ-NH) and surgeon's knot formed by clamping the first throw with toothed mosquito forceps (SG-TM), non-toothed mosquito forceps (SG-NTM), or needle holder (SG-NH). Monotonic loading test was performed on each suture-loop. Initial loop tension, ultimate load, load at 3 mm elongation, elongation at failure, stiffness, and failure mode were compared among techniques. …

Expression Profile Analysis Of Micrornas During The Oestrous Cycle Of Qira Black Sheep, Xi Chen, Hanying Chen, Song Jiang, Chaocheng Li, Xiancun Zeng

The Thai Journal of Veterinary Medicine

Ovarian follicular development is closely regulated by a multitude of genes and specific non-coding RNAs (ncRNAs), which are typically altered during the oestrous cycle. Identification of the microRNAs (miRNA) expression profiles in sheep ovaries during the oestrous cycle is a prerequisite to further understanding their functional status. This study presents a genome-wide analysis of miRNAs from three groups of Qira black sheep: oestrus (day 0), dioestrus (day 10) and pro-oestrus (day 14). A total of 545 conserved miRNAs and 206 novel miRNAs were identified. Furthermore, 229 differentially expressed miRNAs were screened in three comparisons and 16 miRNAs were found to …

Zinc Supplementation Improves Semen Quality In Boars, Supitcha Kaewma, Sanya Suphappornchai, Junpen Suwimonteerabutr, Nutthee Am-In, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

One of the causes of infertility in boars is nutritional deficiency. Interestingly, zinc has been used for improving semen quality in boars. Thus, the objective of this study was to investigate the effect of zinc as feed supplementation in order to improve semen quality in infertile boars. Three purebred Pietrain boars with a poor semen quality history were selected for the experiment. The semen samples were collected once a week for 4 weeks before the treatment period and the semen evaluated immediately. Fifteen grams (a total concentration of 150 ppm) of zinc were supplemented daily in the normal feed. The …

Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk

The Thai Journal of Veterinary Medicine

In female pigs, the anti-Müllerian hormone (AMH) produced by granulosa cells of developing follicles plays an important role in ovarian reserves of follicles and serum AMH levels are the biological marker of gilt reproductive performance. In the present study, we determined the AMH expression during follicular growth in replacement gilts culled due to anestrus by immunohistochemical technique. A total of 20 reproductive organs from crossbred Landrace  Yorkshire gilts were collected from swine commercial farms in Thailand and categorized by the reasons for culling, i.e., the anestrus group (n=12) and other causes not related to reproduction as the control group …

Evaluation Of Serum Calcium Differences In Hypertensive Crises And Control Patients: A Randomly Matched Case-Control Study, Ifeanyi Chukwu O. Onor, Rose M. Duchane, Casey J. Payne, Hannah Naquin Lambert, De Maurian M. Mitchner, Robbie A. Beyl, Anh T. Nguyen, Sarah E. Bilbe, Andrea Arriaga White, Mariah W. Johnson, Amber I. Faciane, Emmanuel Kouagou, Stephanie A. Hymel, Bria M. Wates, Asia D. Sanders, Phillip C.B. Vo, Jordan D. Bates, Raven J. Spooner, Christopher J. Gillard, John I. Okogbaa, Daniel F. Sarpong, Rim M. Hadgu, Samuel C. Okpechi, Gabriel I. Onor, Michael C. Okoronkwo, Mihran V. Naljayan, Shane G. Guillory, Shane E. Sanne

School of Medicine Faculty Publications

The role of calcium in blood pressure has been widely studied among hypertensive patients; however, no study has explored the role of calcium in hypertensive crises. The primary objective of this study is to evaluate the differences in serum calcium levels between hypertensive crises patients and a 1:1 random matched controls (age-, sex-, race-, diabetes, and body mass index matched). This study is a single-center, retrospective, chart review, case-control study of patients with hypertensive crises (case group) and patients without hypertensive crises (control group). Patients were included in the case group if they were 18 years of age or older …

Setting The Research Agenda For Nursing Administration And Leadership Science: A Delphi Study, Esther Maria Chipps, M Lindell Joseph, Catherine Alexander, Bret Lyman, Logan Mcginty, Heather Nelson-Brantley, Joy Parchment, Reynaldo R. Rivera, Mary Anne Schultz, Danielle M. Ward, Susan Weaver

Faculty Publications

Objective: The aim of this study was to identify and prioritize research topics for nursing administration and leadership science.

Background: Nursing administration and leadership research priorities should provide a framework for building the science needed to inform practice.

Methods: The Association for Leadership Science in Nursing (ALSN) and American Organization for Nursing Leadership (AONL) Foundation (AONL-F) for Nursing Leadership and Education collaborated on a Delphi study. Initial input on research priority items were received from ALSN and AONL members. National experts participated in a 3-round Delphi study.

Results: Top-ranked priorities included: 1) nurses' health, well-being, resiliency, and safety in the …

Devastating Effect Of Untreated Facial Squamous Cell Carcinoma, Nasim Khalfe, Ya Xu, Elizabeth May Schuele, Doris Lin

Faculty, Staff and Students Publications

Skin cancer develops primarily on areas of sun-exposed skin and can typically be treated if caught early. Here, we present a case of a 57-year-old man who presented with an extremely large facial squamous cell carcinoma with resulting perineural invasion and metastasis to parotid lymph nodes because he was unable to obtain timely treatment.

The 2021 Ipr Future Of Communications In Asia Report, Tina Mccorkindale, Sarah Crawshaw, Su Lin Yeo, Stephen Thomas, Alexis B. Fitzsimmons, Pang, A.

Research Collection Lee Kong Chian School Of Business

The communication function within Asian-headquartered organizations is undergoing significant growth and changes that will be pivotal for its future success, according to a new Institute for Public Relations (IPR) research report. The “IPR Future of Communications in Asia” research report investigates key changes that will impact the future of the communications function. The analysis is based on interviews with 30 senior communications executives based in Asia in a variety of industries from 2019 to 2020, and three focus groups with nearly 20 senior communication leaders in late 2020.

Personality Pathology And The Dark Side Of Buyer–Supplier Relationships, Simon Croom, Katarina Fritzon, Nathan Brooks

School of Business: Faculty Scholarship

differences amongst the parties to any relationship, thereby ignoring the complex dynamics of human interaction and behaviors on the progress and performance in such relationships. This Notes & Debates paper takes the view that personality matters, particularly toxic personality traits, and argues that for purchasing & supply researchers a cross-disciplinary approach to the study of BSRs will advance our understanding. Our study brought together psychology and supply management researchers. We begin by arguing that much of the extant literature demotes behavior and personality to peripheral, exogenous, or even irrelevant to the study of BSRs. Second, by focusing on the emergent …

A Review Of Fixed Drug Eruption With A Special Focus On Generalized Bullous Fixed Drug Eruption., Hannah J. Anderson, Jason B. Lee, Md

Department of Dermatology and Cutaneous Biology Faculty Papers

Fixed drug eruption (FDE) is a cutaneous adverse drug reaction characterized by the onset of rash at a fixed location on the body each time a specific medication is ingested. With each recurrence, the eruption can involve additional sites. Lesions can have overlying vesicles and/or bullae, and when they cover a significant percentage of body surface area, the eruption is referred to as generalized bullous fixed drug eruption (GBFDE). Due to the widespread skin denudation that can be seen in this condition, GBFDE may be confused clinically with Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN). While treatments described for GBFDE include supportive …

Neutrophil-Rich, Noncollagenous 16a Domain-Negative Bullous Pemphigoid Associated With Psoriasis, Morgan E Sussman, Shoshana K Grossman, Sylvia Hsu, Jason B. Lee, Kiran Motaparthi

Department of Dermatology and Cutaneous Biology Faculty Papers

No abstract provided.

Student Research Mentorship Programme: A Novel Solution For Promoting Research In Medical School, Inaara Akbar, Russell S. Martins, Syeda Sadia Fatima

Medical College Documents

No abstract provided.

Association Of Maternal Prenatal Selenium Concentration And Preterm Birth: A Multicountry Meta-Analysis, Nagendra Monangi, Huan Xu, Waqasuddin Khan, Furqan Kabir, Muhammad Imran Nisar, Aneeta Hotwani, Usma Mehmood, Ambreen Nizar, Javairia Khalid, Fyezah Jehan

Department of Paediatrics and Child Health

Background: Selenium (Se), an essential trace mineral, has been implicated in preterm birth (PTB). We aimed to determine the association of maternal Se concentrations during pregnancy with PTB risk and gestational duration in a large number of samples collected from diverse populations.
Methods: Gestational duration data and maternal plasma or serum samples of 9946 singleton live births were obtained from 17 geographically diverse study cohorts. Maternal Se concentrations were determined by inductively coupled plasma mass spectrometry analysis. The associations between maternal Se with PTB and gestational duration were analysed using logistic and linear regressions. The results were then combined using …

Predicting Lymph Node Metastasis In Non-Small Cell Lung Cancer: Prospective External And Temporal Validation Of The Hal And Homer Models, Gabriela Martinez-Zayas, Francisco A Almeida, Lonny Yarmus, Daniel Steinfort, Donald R Lazarus, Michael J Simoff, Timothy Saettele, Septimiu Murgu, Tarek Dammad, D Kevin Duong, Lakshmi Mudambi, Joshua J Filner, Sofia Molina, Carlos Aravena, Jeffrey Thiboutot, Asha Bonney, Adriana M Rueda, Labib G Debiane, D Kyle Hogarth, Harmeet Bedi, Mark Deffebach, Ala-Eddin S Sagar, Joseph Cicenia, Diana H Yu, Avi Cohen, Laura Frye, Horiana B Grosu, Thomas Gildea, David Feller-Kopman, Roberto F Casal, Michael Machuzak, Muhammad H Arain, Sonali Sethi, George A Eapen, Louis Lam, Carlos A Jimenez, Manuel Ribeiro, Laila Z Noor, Atul Mehta, Juhee Song, Humberto Choi, Junsheng Ma, Liang Li, David E Ost

Faculty, Staff and Students Publications

BACKGROUND: Two models, the Help with the Assessment of Adenopathy in Lung cancer (HAL) and Help with Oncologic Mediastinal Evaluation for Radiation (HOMER), were recently developed to estimate the probability of nodal disease in patients with non-small cell lung cancer (NSCLC) as determined by endobronchial ultrasound-transbronchial needle aspiration (EBUS-TBNA). The objective of this study was to prospectively externally validate both models at multiple centers.

RESEARCH QUESTION: Are the HAL and HOMER models valid across multiple centers?

STUDY DESIGN AND METHODS: This multicenter prospective observational cohort study enrolled consecutive patients with PET-CT clinical-radiographic stages T1-3, N0-3, M0 NSCLC undergoing EBUS-TBNA staging. …

Cheyne-Stokes Respiration In A 17-Year-Old Boy Awaiting Heart Transplantation, Nooralam A Rai, Aliva De, Carin Lamm

The Texas Heart Institute Journal

Cheyne-Stokes respiration is a pattern of alternating central apnea and hyperpnea. It is well described in adults with congestive heart failure, but not in children.

We report the case of a 17-year-old boy whose systolic heart failure was complicated by Cheyne-Stokes respiration. He was given supportive therapy until heart transplant, after which his Cheyne-Stokes respiration clinically resolved. Clinicians should be aware of this uncommon condition in pediatric and adolescent patients who have advanced heart failure and irregular breathing.

A Narrative Review Of Interstitial Lung Disease In Anti-Synthetase Syndrome: A Clinical Approach, Naina Sawal, Sanjay Mukhopadhyay, Sheetal Rayancha, Alastair Moore, Puneet Garcha, Anupam Kumar, Viren Kaul

Faculty, Staff and Students Publications

Anti-synthetase syndrome (AS) is a rare autoimmune disorder characterized by the presence of aminoacyl-transfer RNA synthetase antibodies in conjunction with clinical features such as interstitial lung disease (ILD), Raynaud's phenomenon, nonerosive arthritis, and myopathy. AS distinguishes itself from other inflammatory myopathies by its significant lung involvement and rapidly progressive interstitial lung disease (AS-ILD), therefore the management of AS-ILD requires careful clinical, serologic and radiologic assessment. Glucocorticoids are considered the mainstay of therapy; however, additional immunosuppressive agents are often required to achieve disease control. Patient prognosis is highly dependent on early diagnosis and symptom recognition as the antibody profile is thought …

Treatment Options For Managing Obesity In Chronic Kidney Disease, Vishnu P Parvathareddy, Krishiv M Ella, Maulin Shah, Sankar D Navaneethan

Faculty, Staff and Students Publications

PURPOSE OF REVIEW: Obesity is a risk factor for the development and progression of chronic kidney disease (CKD). In this review, we provide a comprehensive overview of various management options (lifestyle intervention, medications, and bariatric surgery) to address obesity in those with CKD.

RECENT FINDINGS: Few clinical trials have examined the benefits of lifestyle modifications in those with preexisting CKD and suggest potential renal and cardiovascular benefits in this population. Yet, superiority of different dietary regimen to facilitate weight loss in CKD is unclear. Although medications could offer short-term benefits and assist weight loss, their safety and long-term benefits warrant …

Exogenous Inter-Α Inhibitor Proteins Prevent Cell Death And Improve Ischemic Stroke Outcomes In Mice, Louise D Mccullough, Meaghan Roy-O'Reilly, Yun-Ju Lai, Anthony Patrizz, Yan Xu, Juneyoung Lee, Aleah Holmes, Daniel C Kraushaar, Anjali Chauhan, Lauren H Sansing, Barbara S Stonestreet, Liang Zhu, Julia Kofler, Yow-Pin Lim, Venugopal Reddy Venna

Faculty, Staff and Students Publications

Inter-α inhibitor proteins (IAIPs) are a family of endogenous plasma and extracellular matrix molecules. IAIPs suppress proinflammatory cytokines, limit excess complement activation, and bind extracellular histones to form IAIP-histone complexes, leading to neutralization of histone-associated cytotoxicity in models of sepsis. Many of these detrimental processes also play critical roles in the pathophysiology of ischemic stroke. In this study, we first assessed the clinical relevance of IAIPs in stroke and then tested the therapeutic efficacy of exogenous IAIPs in several experimental stroke models. IAIP levels were reduced in both ischemic stroke patients and in mice subjected to experimental ischemic stroke when …

Structure And Dynamics Of Photoreceptor Sensory Cilia, Theodore G Wensel, Valencia L Potter, Abigail Moye, Zhixian Zhang, Michael A Robichaux

Faculty, Staff and Students Publications

The rod and cone photoreceptor cells of the vertebrate retina have highly specialized structures that enable them to carry out their function of light detection over a broad range of illumination intensities with optimized spatial and temporal resolution. Most prominent are their unusually large sensory cilia, consisting of outer segments packed with photosensitive disc membranes, a connecting cilium with many features reminiscent of the primary cilium transition zone, and a pair of centrioles forming a basal body which serves as the platform upon which the ciliary axoneme is assembled. These structures form a highway through which an enormous flux of …

Zfta-Rela Dictates Oncogenic Transcriptional Programs To Drive Aggressive Supratentorial Ependymoma, Amir Arabzade, Yanhua Zhao, Srinidhi Varadharajan, Hsiao-Chi Chen, Selin Jessa, Bryan Rivas, Austin J Stuckert, Minerva Solis, Alisha Kardian, Dana Tlais, Brian J Golbourn, Ann-Catherine J Stanton, Yuen San Chan, Calla Olson, Kristen L Karlin, Kathleen Kong, Robert Kupp, Baoli Hu, Sarah G Injac, Madeline Ngo, Peter R Wang, Luz A De León, Felix Sahm, Daisuke Kawauchi, Stefan M Pfister, Charles Y Lin, H Courtney Hodges, Irtisha Singh, Thomas F Westbrook, Murali M Chintagumpala, Susan M Blaney, Donald W Parsons, Kristian W Pajtler, Sameer Agnihotri, Richard J Gilbertson, Joanna Yi, Nada Jabado, Claudia L Kleinman, Kelsey C Bertrand, Benjamin Deneen, Stephen C Mack

Faculty, Staff and Students Publications

Over 60% of supratentorial (ST) ependymomas harbor a ZFTA-RELA (ZRfus) gene fusion (formerly C11orf95-RELA). To study the biology of ZRfus, we developed an autochthonous mouse tumor model using in utero electroporation (IUE) of the embryonic mouse brain. Integrative epigenomic and transcriptomic mapping was performed on IUE driven ZRfus tumors by CUT&RUN, ChIP, ATAC, and RNA sequencing and compared to human ZRfus driven ependymoma. In addition to direct canonical NF-κB pathway activation, ZRfus dictates a neoplastic transcriptional program and binds to thousands of unique sites across the genome that are enriched with Plagl family transcription factor (TF) motifs. ZRfus activates …