Medicine and Health Sciences Commons^™

Role Of Gp120 Glycosylation In Sexual Transmission Of Hiv, Yingxue Sun, Adam Meadows, Najwa Zebian, Eric Arts, Carole Creuzenet

Western Research Forum

Background:

In chronic HIV patients, the viral populations are genetically diverse due to mutations introduced by the viral reverse transcriptase during HIV replication. However, more than 80% new infections result from single transmission founder (TF) viruses; therefore, targeting the TFs is key to control AIDS worldwide.

Gp120 is a glycosylated envelope protein required for HIV infection, propagation, and transmission. Glycans on gp120 influence HIV infectivity through their interactions with lectins, the carbohydrate-binding immune proteins in the host mucosa. To transmit sexually, viruses must overcome the lectin traps to access more target T cells.

Hypothesis:

TF viruses are less likely to …

The Healthesteps™ Lifestyle Prescription Program To Improve Physical Activity And Modifiable Risk Factors For Chronic Disease: A Pragmatic Randomized Controlled Trial., Dawn P. Gill, W Blunt, N C Boa Sorte Silva, C Stiller-Moldovan, G Y Zou, R J Petrella

Family Medicine Publications

BACKGROUND: Our objective was to determine the influence of the HealtheSteps™ lifestyle prescription program on physical activity and modifiable risk factors for chronic disease in individuals at risk.

METHODS: One hundred eighteen participants were recruited from 5 sites in Southwestern Ontario, Canada and randomized to either the intervention (HealtheSteps™ program, n = 59) or a wait-list control group (n = 59). The study comprised three phases: an Active Phase (0 to 6 months) consisted of bi-monthly in-person lifestyle coaching with access to a suite of eHealth technology supports (Heathesteps app, telephone coaching and a private HealtheSteps™ social network) followed by …

Transcriptional Profiling Unveils Type I And Ii Interferon Networks In Blood And Tissues Across Diseases., Akul Singhania, Christine M Graham, Leona Gabryšová, Lúcia Moreira-Teixeira, Evangelos Stavropoulos, Jonathan M Pitt, Probir Chakravarty, Annika Warnatsch, William J Branchett, Laura Conejero, Jing-Wen Lin, Sophia Davidson, Mark S Wilson, Gregory Bancroft, Jean Langhorne, Eva Frickel, Abdul K Sesay, Simon L Priestnall, Eleanor Herbert, Marianna Ioannou, Qian Wang, Ian R Humphreys, Jonathan Dodd, Peter J M Openshaw, Katrin D Mayer-Barber, Dragana Jankovic, Alan Sher, Clare M Lloyd, Nicole Baldwin, Damien Chaussabel, Venizelos Papayannopoulos, Andreas Wack, Jacques Banchereau, Virginia M Pascual, Anne O'Garra

Faculty Research 2019

Understanding how immune challenges elicit different responses is critical for diagnosing and deciphering immune regulation. Using a modular strategy to interpret the complex transcriptional host response in mouse models of infection and inflammation, we show a breadth of immune responses in the lung. Lung immune signatures are dominated by either IFN-γ and IFN-inducible, IL-17-induced neutrophil- or allergy-associated gene expression. Type I IFN and IFN-γ-inducible, but not IL-17- or allergy-associated signatures, are preserved in the blood. While IL-17-associated genes identified in lung are detected in blood, the allergy signature is only detectable in blood CD4⁺ effector cells. Type I IFN-inducible …

Prevalence And Correlates Of Diagnosed And Undiagnosed Hypertension In The Indigenous Kuna Population Of Panamá, Daniel R. Hanna, Rebekah J. Walker, Brittany L. Smalls, Jennifer A. Campbell, Aprill Z. Dawson, Leonard E. Egede

Center for Health Services Research Faculty Publications

Background: To determine the prevalence of hypertension and investigate sociodemographic correlates in an indigenous Kuna community living on the San Blas islands of Panama.

Methods: Data was collected from adults using a paper-based survey using a cross sectional study design. Blood pressure was measured, and hypertension defined at two cut-points: 130/80 mmHg and 140/90 mmHg. Individuals with undiagnosed hypertension had a blood pressure measurement that indicated hypertension, however, the individual had not been told by a doctor they had hypertension. Whereas individuals with diagnosed hypertension had been told by a healthcare provider that they had hypertension. Univariate tests compared diagnosed …

The Role Of The Community Health Delivery System In The Health And Well-Being Of Justice-Involved Women: A Narrative Review, Sharla A. Smith, Glen P. Mays, Tracie C. Collins, Megha Ramaswamy

Health Management and Policy Faculty Publications

Background: Over seven million imprisoned and jailed women are released into the community each year and many are ill-equipped to meet the challenges of re-integration. Upon release into their community, women are faced with uncertain barriers and challenges using community services to improve their health and well-being and reuniting with families. Few studies have identified and described the barriers of the community health delivery system (CHDS)- a complex set of social, justice, and healthcare organizations that provide community services aimed to improve the health and well-being (i.e. safety, health, the success of integration, and life satisfaction) of justice-involved women. We …

Epilepsy Diagnosis And Management Of Children In Kenya: Review Of Current Literature, Pauline Samia, Jane Hassell, Jessica-Anne Hudson, Symon M. Kariuki, Charles R. Newton, Jo M. Wilmshurst

Paediatrics and Child Health, East Africa

Introduction: The growing impact of non-communicable diseases in low- to middle-income countries makes epilepsy a key research priority. We evaluated peer-reviewed published literature on childhood epilepsy specific to Kenya to identify knowledge gaps and inform future priorities.

Methodology: A literature search utilizing the terms “epilepsy” OR “seizure” as exploded subject headings AND “Kenya” was conducted. Relevant databases were searched, generating 908 articles. After initial screening to remove duplications, irrelevant articles, and publications older than 15 years, 154 papers remained for full-article review, which identified 35 publications containing relevant information. Data were extracted from these reports on epidemiology, etiology, clinical features, …

Developing Interactive Curricular Elements With Teams Of Faculty, Staff And Students, Betsy J. Becker, Sara Bills, Robert H. Fuchs, Kellie Gossman, Tessa Wells, Peggy Moore

Posters and Presentations: Physical Therapy

Students are continually searching for new resources to augment their learning. Online resources have become highly favored, especially by the millennial age group, for convenience, self-paced content, and versatility across learning styles. It has been reported that e-learning platforms are as effective as traditional face-to-face instruction, but may be best utilized when used as an adjunctive resource for teaching psychomotor skills.1,2 In a study about student confidence, results showed an increase in self-efficacy following the use of online learning materials.3 Blended learning, the next generation of web-based education, mixes advances in technology with creative faculty to fuse effective delivery methods …

Phospholipases D: Making Sense Of Redundancy And Duplication, Andrew J. Morris

Internal Medicine Faculty Publications

Why have two genes when one would suffice? Evolutionary pressure means that biology, unlike government, is generally intolerant of wasted effort. Therefore, when multiple genes exist presumably they are there to provide some benefit to the organism even if that benefit is not immediately obvious to us scientists. A recent report from Raghu and colleagues (Biosci. Rep. (2018) 38, pii: BSR20181690) [1] sheds some light on one possible reason for the existence of two Phospholipases D genes in chordates when only one is present in invertebrates.

Keefektifan Achievement Motivation Training Untuk Meningkatkan Motivasi Berprestasi Siswa Sekolah Menengah Pertama, Edwindhana Mareza Putra, Dany M. Handarini

Jurnal Kajian Bimbingan dan Konseling

The particular study aimed to determine the effectiveness of achievement motivation training (AMT) in increasing the achievement motivation of junior high school students. The study used an interrupted time-series design which conducted on an intact group consisting of eight subjects. Data measurements carried out three times in the pre-test and post-test conditions with six forms of achievement motivation scale. The AMT intervention in the psychoeducation frame consists of two strategies, namely achievement thinking and action strategies which are carried out in 12 sessions. The results showed that there is a difference between achievement motivation scores before and after AMT interventions.

Keberfungsian Model Abc Tschudi Untuk Internalisasi Budaya Belajar Mahasiswa, Andi Mappiare-At, Lutfi Fauzan

Jurnal Kajian Bimbingan dan Konseling

This research aimed to find: (1) the difficulties possibly faced by subjects in managing personal construct when filling Tschudi ABC format; (2) key personal construct recognized by subjects in their present and desired positions; (3) the results of subjects’ learning culture internalization to the implementation of Tschudi ABC Model. This study used a qualitative approach with the type of Classroom Action Research, the empirical phenomenological variant. The observed subjects were determined by purposive sampling, taken from the students of Multicultural Counseling class. The research informants were determined through snowballing. The data collection was conducted via a medium participatory observation and …

Pengaruh Kecerdasan Dan Minat Pribadi Sosial Terhadap Penyesuaian Diri Mahasiswa Bimbingan Dan Konseling, Nina Permata Sari, Ririanti Rachmayanie

Jurnal Kajian Bimbingan dan Konseling

The objective of this research is to identify the influence of intelligence and social self interest on the adaptation skill of the department of guidance and counseling students. This quantitative research used the multiple regression analysis. It used purposive total sampling technique, with sample of 38 students of Department of Guidance and Counseling, Faculty of Teacher Training and Education, Universitas Lambung Mangkurat (ULM). The results show that intelligence and social self interest of the department of guidance and counseling students simultaneously influence the adaptation skill of 35.9%, while the remaining 64.1% is influenced by other factors.

Astragaloside Iv Protects Neurons From Microglia-Mediated Cell Damage Through Promoting Microglia Polarization., Jingwen Yu, Minfang Guo, Yanhua Li, Huiyu Zhang, Zhi Chai, Qing Wang, Yuqing Yan, Jiezhong Yu, Chunyun Liu, Guang-Xian Zhang, Ma Cungen

Department of Neurology Faculty Papers

Astragaloside IV (AST-IV) is a major active ingredient of astragalus, with a neuroprotective effect. The current study is aimed to investigate the impact of AST-IV on the M1/M2 microglial activation in response to lipopolysaccharide (LPS) stimulation, how AST-IV attenuated microglia-mediated neuronal damage, and the molecular mechanisms underlying AST-IV's protection of neurons against microglia-mediated neuronal damage. Our results showed that AST-IV partially protected microglia from death evoked by LPS and downregulated the release of pro-inflammatory (M1) mediators including interleukin (IL)-1β, IL-6, tumour necrosis factor α (TNF-α) and nitric oxide, as well as the expression of Toll-like receptors 4 (TLR4), MyD88, and …

Neural Foundations Of Ayres Sensory Integration, Shelly J. Lane, Zoe Mailloux, Sarah Schoen, Anita Bundy, Teresa A. May-Benson, L. Diane Parham, Susanne Smith Roley, Roseann C. Schaaf

Department of Occupational Therapy Faculty Papers

Sensory integration, now trademarked as Ayres Sensory Integration^® or ASI, is based on principles of neuroscience and provides a framework for understanding the contributions of the sensory and motor foundations of human behavior. The theory and practice of ASI continues to evolve as greater understanding of the neurobiology of human behavior emerges. In this paper we examine core constructs of ASI identified in the seminal work of Dr. Jean Ayres, and present current neuroscience research that underlies the main patterns of sensory integration function and dysfunction. We consider how current research verifies and clarifies Ayres’ propositions by describing functions …

Survival, Fidelity, And Dispersal Of Double-Crested Cormorants On Two Lake Michigan Islands, Christopher R. Ayers, Katie C. Hanson-Dorr, Ken Stromborg, Todd W. Arnold, Jacob S. Ivan, Brian S. Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Colony fidelity and dispersal can have important consequences on the population dynamics of colonial-nesting birds. We studied survival and inter-colony movements of Double-crested Cormorants (Phalacrocorax auritus; cormorants) nesting at Spider and Pilot islands, located 9 km apart in western Lake Michigan, during 2008–2014. We used live resighting and dead recovery data from both colonies, plus dead recoveries from throughout North America, in a multistate live and dead encounter model to estimate annual survival, inter-colony movements, plus temporary and permanent emigration to unmonitored sites. Annual survival averaged 0.37 (annual process variation, ˆ = 0.07) for hatch-year, 0.78 (ˆ= 0.08) for secondyear, …

Full Issue, Volume 7, Number 2, Benjamin Silliman, Scott Cummings, Donna J. Peterson, Rich Poling

Journal of Human Sciences and Extension

No abstract provided.

Understanding The Implementation Of Integrated Care For People Living With Hiv, Vickie A. Lynn

USF Tampa Graduate Theses and Dissertations

Introduction: The integration of physical and behavioral health services is an innovative method of delivering health care services for persons dual or triply diagnosed with HIV, mental health and/or substance use disorders. Reducing the fragmented delivery of health services by integrating services into one setting is essential to providing holistic patient-centered care and address the complex health needs of this population. Although research shows integrated care improves health outcomes, little is known about the implementation of integrated care in real-world settings because the perspectives and experiences of those delivering and receiving integrated health and social services are underrepresented in the …

Keefektifan Program Adventure Based Counseling Untuk Meningkatkan Kebahagiaan Mahasiswa, Aprilia Setyowati, Siti Partini Suardiman, Mufied Fauziah

Jurnal Kajian Bimbingan dan Konseling

A happy individual tends to produce positive things in life in terms of social self; study; and career aspects. This research is to find out the effectiveness of adventure based counseling (ABC) program to improve happiness in students. This research was conducted using experimental design with one group pretest-posttest model. The subject of this research was eleven students in the third semester of study program of guidance and counseling, Universitas Ahmad Dahlan (UAD) who were selected using purposive sampling technique. The pretest and posttest data was collected using a scale which was developed based on Seligman theory. The data was …

Developing Learning Guidance And Counseling Program Based On Social And Emotional Learning In Senior High School, Muhammad Farozin, Luky Kurniawan

Jurnal Kajian Bimbingan dan Konseling

This research and development aims to develop a learning guidance and counseling program based on social and emotional learning (SEL) in senior high school, and find out the feasibility of the learning guidance and counseling program based on SEL. Approach used in this research was research and development. The development procedure refers to the procedure by Borg & Gall. The two subject groups of program validation in this research and development were two expert validators who were competent in the learning guidance and counseling program and the school counselor group. Data collection instruments used were expert validation questionnaire; practitioner validation …

Evaluation Of Clinical Outcomes In An Interdisciplinary Abdominal Pain Clinic: A Retrospective, Exploratory Review., Amanda D. Deacy, Craig A. Friesen, Vincent S. Staggs, Jennifer Verrill Schurman

Manuscripts, Articles, Book Chapters and Other Papers

Background: Pediatric functional gastrointestinal disorders (FGIDs) are common and well-accepted to be etiologically complex in terms of the contribution of biological, psychological, and social factors to symptom presentations. Nonetheless, despite its documented benefits, interdisciplinary treatment, designed to address all of these factors, for pediatric FGIDs remains rare. The current study hypothesized that the majority of pediatric patients seen in an interdisciplinary abdominal pain clinic (APC) would demonstrate clinical resolution of symptoms during the study period and that specific psychosocial variables would be significantly predictive of GI symptom improvement.

Aim: To evaluate outcomes with interdisciplinary treatment in pediatric patients with pain-related …

Galcanezumab In Episodic Migraine: Subgroup Analyses Of Efficacy By High Versus Low Frequency Of Migraine Headaches In Phase 3 Studies (Evolve-1 & Evolve-2)., Stephen D. Silberstein, Virginia L. Stauffer, Katie A. Day, Sarah Lipsius, Maria-Carmen Wilson

Department of Neurology Faculty Papers

BACKGROUND: Patients with high-frequency episodic migraine (HFEM) have a greater disease burden than those with low-frequency episodic migraine (LFEM). Acute treatment overuse increases the risk of migraine chronification in patients with HFEM. Galcanezumab, a humanized monoclonal antibody binding calcitonin gene-related peptide (CGRP), is effective for migraine prevention with a favorable safety profile. Here, we investigate whether there are differences in galcanezumab efficacy in patients with LFEM or with HFEM.

METHODS: Data were pooled from two double-blind, placebo-controlled phase 3 trials; EVOLVE-1 and EVOLVE-2. Patients were 18-65 years old, experienced 4-14 monthly migraine headache days (MHDs) for ≥1 year prior, with …

Us Integrated Delivery Networks Perspective On Economic Burden Of Patients With Treatment-Resistant Depression: A Retrospective Matched-Cohort Study., Dominic Pilon, Holly Szukis, Kruti Joshi, David Singer, John J. Sheehan, Jennifer W. Wu, Patrick Lefebvre, Paul Greenberg

College of Population Health Faculty Papers

OBJECTIVE: Our objective was to assess healthcare resource utilization (HRU) and costs among patients with major depressive disorder (MDD) with and without treatment-resistant depression (TRD) and those without MDD in US Integrated Delivery Networks (IDNs).

METHODS: This was a retrospective matched-cohort study. The Optum© Integrated Claims Electronic Health Record de-identified database was used to identify adult patients with TRD (January 2011-June 2017) across US IDNs. TRD patients were propensity score matched 1:1 with non-TRD MDD and non-MDD patients on demographics. Rates of HRU and costs were compared up to 2 years following the first antidepressant pharmacy claim (or randomly imputed …

An Application Of The Modifiable Areal Unit Problem: Optimizing Cluster Method Parameters To Produce Predictive Data For Hiv Outbreaks, Connor J. Chato, Art Fy Poon

Western Research Forum

Background

A popular approach to study HIV outbreaks is to cluster cases based on genetic similarity. However, there is no widely-used statistical criterion which optimizes the parameters for sequence-based clustering methods. The relationship between a cluster-defining similarity threshold and it’s associated set of clusters can be analogized to the aggregation level in the Modifiable Areal Unit Problem (MAUP).

Hypothesis

Based on the selection of aggregation level for study partitions in MAUP, we present a statistical framework to optimize the similarity threshold for pairwise distance algorithm TN93 (http://github.com/veg/tn93). We hypothesize that defining this threshold includes case connections such that …

The Wet Bridge Transfer System: An Novel In Vitro Tool For Assessing Exogenous Surfactant As A Pulmonary Drug Delivery Vehicle, Brandon J. Baer

Western Research Forum

Background:

Due to its complex branching structure, direct drug delivery to the remote areas of the lung is a major challenge. Consequently, most therapies, such as those treating pulmonary infection and inflammation, must utilize large systemic dosing, with the potential for adverse side effects. A novel alternative strategy is to use exogenous surfactant, a material capable of distributing throughout the lung, as a pulmonary drug delivery vehicle.

Objective:

Utilize an in vitro transferring system to assess exogenous surfactant (BLES) as a pulmonary delivery vehicle for different therapeutics.

Methods:

An in vitro technique was developed to simultaneously study surfactant delivery and …

Psychometric Properties Of Standardized Balance Confidence, Fear Of Falling, And Falls-Efficacy Measures In People With Lower Limb Amputations, Jack Y. Wang, Susan W. Hunter, Ricardo Viana, Jeffrey D. Holmes, Michael Payne

Western Research Forum

Background:

In Canada, >50% of community-dwelling lower limb amputees (LLA) fall at least once each year, a rate that is almost twice that of community-dwelling older adults. While the physical consequences of falls may be readily apparent, psychological sequelae that follow may be just as, if not more, detrimental than an actual fall itself. Current measures of balance confidence show no change in LLA following discharge from rehabilitation. The limited detectable change may be due to content validity challenges of the measures as they were not developed for the unique challenges faced by LLA.

Objectives:

1) Review items from standardized …

Depression Affects Recovery Following Distal Radius Fracture: A Latent Class Analysis, Shirin Modarresi, David M. Walton, Joy C. Macdermid

Western Research Forum

Background: Most people recover within six months following distal radius fractures (DRFs) but some experience pain and disability for one year or longer. Therefore, it is important to understand the factors that can help predict recovery. According to the biopsychosocial model of pain, psychological aspects of a condition can play important roles in explaining recovery.

Objectives: To identify the recovery trajectories of patients with DRFs and to determine the degree to which depression affects these trajectories.

Methods: Recovery was assessed in 318 patients using the Patient-Rated Wrist Evaluation scale at baseline, three, six, and 12 months. Demographic information was collected …

Where On Ice? Algorithmically Deconstructing Nhl Shot Locations As A Method For Player Classification, Devan Becker, Douglas G. Woolford, Charmaine B. Dean

Western Research Forum

Where do hockey players shoot from? How does this vary from player to player? We present the results of a study that uses data-driven statistical methods to investigate these questions. The locations of shots by National Hockey League (NHL) players from 2011 to 2017 are analyzed using a combination of an image recognition algorithm and spatial statistical methodology. An unsupervised classifier is applied to output from a spatial point process model in order to determine which shot locations best characterize a given player. We define the number of regions a priori, but the image recognition algorithm chooses the shape …

Phylogenetic Estimates Of Hiv-1 Gp120 Indel Rates Across The Group M Subtypes, John Palmer, Art Poon

Western Research Forum

Insertions and deletions (indels) in the HIV-1 envelope glycoprotein gp120 play a significant role in the evolution of HIV pathogenesis and transmission fitness. While substitution rates in HIV-1 are well characterized by phylogenetic models, there is a lack of quantitative measures of indel rates in HIV-1. Here we use a dated-tip phylogenetic analysis of gp120 sequences to estimate indel rates for 7 subtypes and CRFs of HIV-1 group M.

We obtained and processed 26,359 HIV-1 gp120 sequences from the Los Alamos National Laboratory HIV Sequence database. After filtering these sequences, we extracted the conserved and variable regions from the remaining …

Mouse Performance On A Novel Touchscreen Continuous Performance Task Is Dependent On Signaling In The Prelimbic Cortex, Tyler D. Dexter, Daniel Palmer, Amy C. Reichelt, Anita Taksokhan, Lisa M. Saksida, Tim J. Bussey

Western Research Forum

Attention is the cognitive processing that facilitates the ability to target and attend to relevant environmental stimuli, while filtering out irrelevant or distracting stimuli. Control over selective attention is theorized to be dependent on organized neural communication that stems from the medial prefrontal cortex (mPFC). To evaluate selective and sustained attention, mice were trained on the novel touchscreen rodent continuous performance task (rCPT), a task designed to emulate the human CPT. In the rodent version, images are continuously presented on a touchscreen, where mice have been trained to selectively respond to one image type while suppressing responses to all others. …

Dairy Intake And Cognitive Function In Canadian Older Adults, Mariam R. Ismail Miss, Alan Salmoni

Western Research Forum

Background: Dietary intake is one of the modifiable factors that may affect older adults’ cognitive function in their later years. Few research has considered the potential role of dairy foods on cognitive function. Methods: Across-sectional study was undertaken in 2014. Cognitive function was assessed using The Montreal Cognitive Assessment (MoCA), Rey Complex Figure Test and Recognition Trial (RCFT), Trail-Making Test (TMT), Victoria Stroop Test (VST) and the Digit Span Test (DST). Dietary intake was assessed via estimated 5-day food intake records and analyzed for saturated fat, vitamin D and calcium. Results: A total of 32 participants (8 males and 24 …

Using Molecular Dynamics To Study Qs21 Interactions And Penetration Of Lipid-Cholesterol Bilayers, Sarai Guerrero, Mikko Karttunen

Western Research Forum

Saponins have been used as adjuvant agents for decades in vaccines and therapies, but none are as well studied or heavily used as QS-21. This achievement is notwithstanding the fact that QS-21 usage is limited by its stability, toxicity, and scarcity. These shortcomings have only pushed researchers to develop and experiment with artificial recreations of the saponin to harness its unique benefits. A considerable number of research hours have been poured into this topic, but like QS-21 there is a shortcoming here as well. The number of articles that look at QS-21 interactions with the bilayer or the conditions under …