Medicine and Health Sciences Commons^™

The Effects Of Submaximal Exercise Training On Cardiovascular Functions And Physical Capacity In Dogs With Myxomatous Mitral Valve Disease, Saikaew Sutayatram, Chollada Buranakarl, Anusak Kijtawornrat, Kumpanart Soontornvipart, Pakit Boonpala, Prapawadee Pirintr

The Thai Journal of Veterinary Medicine

Myxomatous mitral valve disease (MMVD), an important disease in senile small breed dogs, leads to deterioration of cardiovascular function and impairment of functional capacity resulting in poor quality of life. Submaximal treadmill running has been used as both cardiovascular training and testing worldwide in humans and recently in animals. The objectives of this study were to examine the effects of submaximal endurance training (ET) by treadmill running on safety, adverse events, cardiovascular adaptations, hematology, functional capacity, and the feasibility of submaximal treadmill running test and 6-minute walk test (6-MWT) in dogs with MMVD. Six adult beagle dogs with MMVD, stage …

Effects Of Coenzyme Q10 Supplementation On Cardiac Troponin I Level, Heart Rate Variability, And Echocardiographic Profiles In Canine With Myxomatous Degenerative Mitral Valve Disease: A Pilot Study, Kittipong Tachampa, Tuchakorn Lertwanakarn, Panupong Atchariyasakchai, Vichayanee Pumpitakkul, Supapatt Kireewan, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

The clinical benefits of Coenzyme Q10 (CoQ10) have been well documented in heart failure patients. In veterinary medicine, however, the clinical benefits of CoQ10 have not been well established in dogs with heart diseases. This study aimed to determine the effects of CoQ10 supplementation on 1) preventing myocardial injury 2) cardiac function 3) autonomic balance in dogs with myxomatous degenerative mitral valve disease (MMVD). Thirteen dogs with MMVD at ACVIM stage C were recruited in this study. Dogs were further divided into group I (Body weight [BW] < 6 kg.; n = 7) and group II (BW ≥ 6 kg.; n = 6). Cardiac troponin I (cTnI), echocardiographic examination, and 2 hours of Holter recording were performed on Day 0 and after receiving CoQ10 at 100 mg/dog twice daily for 28 days. CoQ10 caused a reduction of cTnI level in 71% of the dogs. Median cTnI tended to cause tended to decline but not significantly. Systolic function [i.e. fractional shortening (FS) and ejection fraction (EF)] increased significantly by 8 % and 9% (P < 0.05), respectively in group I after CoQ10 supplementation. Other echocardiographic parameters were not altered in either group. HRV analysis revealed no change in autonomic function and balance. cTnI negatively correlated with FS (P < 0.01) and positively correlated with LF/HF ratio (P < 0.05). In conclusion, this study demonstrated the clinical benefits of CoQ10 supplementation in dogs with MMVD. CoQ10 improves cardiac function in small dogs with MMVD. Finally, supplementation of CoQ10 in dogs should be based on body weight.

Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Classical swine fever virus (CSFV) contributes to economic loss of swine production in endemic countries. Serum neutralization and enzyme-linked immunosorbent assay (ELISA) are serological tests commonly used for monitoring CSFV antibody status with serum samples. This experiment evaluated the detection of CSFV antibody in oral fluid samples in commercial ELISA test kit by in vitro study of negative oral fluid mixed with serum of known CSFV serum neutralizing (SN) titer and in vivo oral fluid samples obtained from experimental animals. Correlations of SN titer and S/P ratio of ELISA were observed with the in vitro oral fluid samples, indicating the …

Molecular Characterization Of Trichuris Ovis From Goats In Hunan Province Of China, Jia-Min Tang, Lu-Lin Song, Lin-Feng Nie, Fen Li

The Thai Journal of Veterinary Medicine

Trichuris ovis (Nematoda: Trichuridae) is a widespread nematode that can infect a broad range of animal hosts, such as goats, sheep and oryx. This parasite is of vital importance as a pathogen, but its population genetics, molecular epidemiology and biology are still not well understood. In this study, T. ovis isolates were collected from goats in Hunan province, China, and sequences variation in cox1 gene were examined. A portion of cox1 gene from each collected adult T. ovis individual was separately amplified using the PCR method, and the amplicons were sequenced from both directions. The sequences of pcox1 gene were …

Ecg Quiz, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

A nine year old intact male Miniature Pinscher weighing 4.8 kilogram was presented to veterinarian with signs of severe coughing and exercise intolerance. He was diagnosed of heart disease and previously described the angiotensin converting enzyme inhibitor and diuretic for a period of 6 months. Neither signs of syncope nor seizure was found. Physical examination revealed the dog was depressed and lethargy. The electrocardiography was performed and shown in Figure 1. The blood test showed normal complete blood count with normal kidney and liver blood profiles. The blood parasite was also negative. The thoracic radiograph revealed the whole heart enlargement …

What Is Your Diagnosis?, Ninlawan Thammasiri, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

The patient was showed up to the diagnostic imaging unit, The Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University due to the chief complains of left forelimb swelling after the dog was hit by car for one day duration.

September 2018, Swosu Bulldog Wellness Committee

SWOSU BULLDOG WELLNESS

Walking Club
The SWOSU Walking Club is meeting every MWF, 12:10 pm, at the Wellness Center. Fit exercising into your workday with a 30-40-minute walk and receive the benefits of social support, stress relief, and renewed energy.

SWOSU Fall Pool Hours
Monday-Friday
6:00-8:00 a.m. 12:00-2:00 p.m.
5:00-7:00 p.m. (except Tuesday)

Water Aerobics
Monday-Wednesday 6:00–7:00 p.m.
Thursday 5:00-6:00 p.m.

Giant Bilateral Adrenal Myelolipomas In Two Chinese Families With Congenital Adrenal Hyperplasia, Qiuli Liu, Lin-Ang Wang, Jian Su, Dali Tong, Weihua Lan, Luofu Wang, Gaolei Liu, Jun Zhang, Victor Wei Zhang, Dianzheng Zhang, Rongrong Chen, Qingyi Zhu, Jun Jiang

PCOM Scholarly Works

CONTEXT AND OBJECTIVES: Congenital adrenal hyperplasia (CAH) is one of the most prevalent, and potentially severe, genetic inborn errors of steroid synthesis directly affecting metabolism. Most patients are diagnosed and treated at an early age. There have been very limited reports of adults with CAH-associated adrenal myelolipomas. We aimed to analyze two families with CAH-associated giant adrenal myelolipomas caused by defects in CYP21A2 and CYP17A1 genes.

PARTICIPANTS AND METHODS: A total of 14 individuals from two unrelated families were identified with either CYP21A2 or CYP17A1 mutations. Of note, 5 patients were found with adrenal myelolipomas. Total DNA isolated from the …

Bridging Theory To Practice: Utilizing The Culture-Centered Approach (Cca) To Address Gaps In Community Based Participatory Research (Cbpr) Processes, Sydney Dillard, Agaptus Anaele, Rati Kumar, Raihan Jamil

All Works

The purpose of this study is to provide recommendations to bridging the "theoretical" with the "practical" in developing community-based participatory research (CBPR) health communication projects. As illustrated through a review of several case studies from health campaigns using CBPR, often times the theoretical orientations of CBPR become secondary to its praxis, with unspoken motives and agendas become motivating factors in guiding the initiatives. These motives may come in the form of funding organization priorities, funded grant proposal constraints, and the desire to continue relationships that are fostered in the development of CBPR projects. In response, this essay reintroduces the culture-centered …

A Disturbance In The Force: Cellular Stress Sensing By The Mitochondrial Network, Robert Gilkerson

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

As a highly dynamic organellar network, mitochondria are maintained as an organellar network by delicately balancing fission and fusion pathways. This homeostatic balance of organellar dynamics is increasingly revealed to play an integral role in sensing cellular stress stimuli. Mitochondrial fission/fusion balance is highly sensitive to perturbations such as loss of bioenergetic function, oxidative stress, and other stimuli, with mechanistic contribution to subsequent cell-wide cascades including inflammation, autophagy, and apoptosis. The overlapping activity with m-AAA protease 1 (OMA1) metallopeptidase, a stress-sensitive modulator of mitochondrial fusion, and dynamin-related protein 1 (DRP1), a regulator of mitochondrial fission, are key factors that shape …

Early Investigations And Recent Advances In Intraperitoneal Immunotherapy For Peritoneal Metastasis., Anusha Thadi, Marian Khalili, William F. Morano, Scott D. Richard, Steven C. Katz, Wilbur B. Bowne

Department of Obstetrics and Gynecology Faculty Papers

Peritoneal metastasis (PM) is an advanced stage malignancy largely refractory to modern therapy. Intraperitoneal (IP) immunotherapy offers a novel approach for the control of regional disease of the peritoneal cavity by breaking immune tolerance. These strategies include heightening T-cell response and vaccine induction of anti-cancer memory against tumor-associated antigens. Early investigations with chimeric antigen receptor T cells (CAR-T cells), vaccine-based therapies, dendritic cells (DCs) in combination with pro-inflammatory cytokines and natural killer cells (NKs), adoptive cell transfer, and immune checkpoint inhibitors represent significant advances in the treatment of PM. IP delivery of CAR-T cells has shown demonstrable suppression of tumors …

Comparison Of Dried Blood Spot, Buccal Swab, Cloacal Swab And Feces As Dna Sources To Identify Avian Sexes By Pcr, Sariya Asawakarn, Intuorn Teeranuwat, Natchaya Watcharaprapapong, Nutta Siriwatchaiporn, Panithan Somsai, Mullika Kuldee, Gunnaporn Suriyaphol, Sirakarnt Dhitavat

The Thai Journal of Veterinary Medicine

Absence of sexual dimorphism makes it difficult to determine bird’s sex. A number of both invasive and noninvasive methods as a DNA template of sampling for avian sexing can be used. The aim of this study was to compare the efficacy of different sources of DNA for avian sexing by PCR. Samples were collected from dried blood spots, buccal swabs, cloacal swabs and feces. The highest success rate for sexing determination was from the dried blood spots, accounting for 100%, while those from the buccal swabs, cloacal swabs and feces were 74%, 75.47% and 29.17%, respectively. The non-urate fecal samples …

Comparison Of Gts And Rapd Assays To Characterize Thai Mycoplasma Gallisepticum Strains, Arithat Limsatanun, Jiroj Sasipreeyajan, Somsak Pakpinyo

The Thai Journal of Veterinary Medicine

Mycoplasma gallisepticum (MG) is one of the important pathogens which have economic impact on the poultry industry worldwide. Molecular characterization is the effective method to study the relation among international strains and the epidemiology of MG transmission. In this study, 17 Thai MG strains were characterized by using 2 molecular assays including random amplified polymorphic DNA (RAPD) and gene-targeted sequencing (GTS) assays. Results showed that the RAPD assay could classify the Thai MG strains into 3 patterns.Most MG strains obtained from the same area were in the same pattern. Partial mgc2 gene was used to distinguish between the Thai MG …

E-Cadherin Localization In Oviduct And Uterine Horn Of Swamp Buffalo During Estrous Cycle, Paisan Tienthai

The Thai Journal of Veterinary Medicine

The main purpose of the present study was to investigate the localization of epithelial cadherin (E-cadherin) in the oviduct and uterine horn of swamp buffalo at the follicular and mid-luteal phases. E-cadherin is a transmembrane glycoprotein which plays a vital role in the mechanism of the maintenance epithelial architecture, cell growth and proliferation, cell to cell adhesion including cell to epithelium adhesion. Female reproductive tracts of swamp buffaloes (n=30) were collected from local abattoirs and categorized into follicular (n=15) and mid-luteal (n=15) phases. Tissue samples from the oviducts and uterine horns were regularly handled according to histological and immunohistochemical techniques. …

Molecular Epidemiology And Antimicrobial Resistance Of Salmonella Spp. Isolated From Broilers And Pigs At Slaughterhouses In Thailand And China, Wenjing Yang, Dusadee Phongaran, Teerarat Prasertsee, Rendong Fang, Patchara Phuektes, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

This study aimed to compare the antimicrobial resistance patterns and DNA restriction patterns by PulsedField Gel Electrophoresis (PFGE) between Salmonella spp. isolated from broilers and pigs in Thailand and China. One hundred and sixty six isolates were collected from broilers and pigs at slaughterhouses in Khon Kaen, Thailand (n=106) between February to August 2017 and in Chongqing, China (n=60) between March to October 2015. Antimicrobial susceptibility of the isolates was determined using the disk diffusion method with nine antimicrobial agents. Genotypic diversity of the isolates used PFGE of Xbal-digested chromosomal DNA to determine. Of 166 Salmonella isolates, 37 serotypes were …

Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri

The Thai Journal of Veterinary Medicine

The peroxisome proliferator-activated receptors (PPARs) belong to nuclear receptor superfamily acting as transcription factors related to lipid and glucose metabolism. There are 3 subtypes including PPARα, PPAR, and PPAR. PPARα and PPAR activations can inhibit tumor cell proliferation and differentiation in many cell types. However, the expressions of these receptors in canine mammary tissue are still unknown. This study was performed to investigate the expressions of PPARα and PPAR in normal, benign, and malignant canine mammary tissues. Twenty four bitches at the age of 5-15 years old, which undergone unilateral mastectomy, were used in the study. Mammary tissues were sectioned …

Syrian Hamster (Mesocricetus Auratus) As An Animal Model For Haplorchis Taichui Infection: Biology, Morphology, And Pathology, Jiraporn Songsri, Thidarut Boonmars, Sirintip Boonjaraspinyo, Ratchadawan Aukkanimart, Pranee Sriraj, Parichart Boueroy, Nadchanan Wongchalee

The Thai Journal of Veterinary Medicine

Haplorchis taichui is a trematode that can mature in the small intestine of mammals and birds, including humans. Larval stages occur in various invertebrate and cold-blooded vertebrate hosts. The purpose of this study was to investigate parasite growth, including worm recovery rate, the body size of adult worms, eggs per worm and eggs per gram of feces and pathological changes of the small intestine in experimental hamsters. In this study, 6- to 8-weekold male Syrian hamsters (Mesocricetus auratus) were each infected with 200 H.taichui metacercariae and then sacrificed at weeks 1, 2, 3, 4, 6 and 8 post-infection. The small …

The Electrocardiogram Of The Conscious Chinese Goose (Anser Cygnoides), Mehmet Kaya, Metin Çenesiz

The Thai Journal of Veterinary Medicine

This study was carried out on 24 healthy adult Chinese goose (Anser cygnoides) to determine normal electrocardiographic values and patterns in standard bipolar and augmented bipolar limb leads. The study between males and females was also performed to investigate changes in the electrocardiographic patterns related to sex. A regular sinus rhythm was observed in all birds. There was no significant difference between male and female geese except for PR interval, therefore, all data was pooled. All waves in lead I had very low amplitude or almost isoelectric. The P waves, in other leads, were mainly positive in lead II, III …

Development Of An Insoluble Hyaluronic Acid Membrane As An Effective Material For The Prevention Of Post-Thoracotomy Pleural Adhesions In Dogs, Akiko Uemura, Takashi Tanaka, Pinkarn Chantawong, Pitipat Kitpipatkun, Ryou Tanaka

The Thai Journal of Veterinary Medicine

A novel membrane was previously found to be effective in preventing post-thoracotomy pleural adhesions in dogs. The aim of the present study was to verify the animals’ clinical condition, the biological fate of the residual membrane, the presence or absence of pleural adhesions, and any inflammatory reactions over a 10-week observation period following membrane implantation. The animals used were two male beagles. The experimental membrane, an insoluble hyaluronic acid membrane containing glycerin, was implanted under general anesthesia above the visceral pleura in the left pleural cavity of each dog after exposure to air for 30 min. A drainage tube was …

Long-Term Effect Of Low-Dose Imatinib Therapy For Pulmonary Hypertension Due To Chronic Degenerative Mitral Valve Disease In Six Dogs, Zi Ping Leong, Shinji Arita, Yoshiaki Hikasa

The Thai Journal of Veterinary Medicine

Six dogs were diagnosed with pulmonary hypertension (PH) secondary to chronic degenerative mitral valve disease (CDMVD) on echocardiography. Imatinib (3 mg/kg, every 24 hours, PO) was initiated without any changes to the background therapy to treat the PH. Follow-up evaluations at 1, 3, 5 and 6 months revealed substantial clinical and hemodynamic improvements. One dog showed deterioration after the imatinib withdrawal to necessitate a restart therapy. No side effects were observed throughout the 6-month treatment course. Low-dose imatinib may provide a promising treatment alternative for CDMVD-associated PH in dogs.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

A nine-year-old, female Shih Tzu had presented with left buphthalmic eye. She had a history of chronic glaucoma (left eye), which had been treating with topical hypotensive medication for several months. Her right eye is normal.

Crispr-Cas9 Mediated Epitope Tagging Provides Accurate And Versatile Assessment Of Myocardin--Brief Report, Qing Lyu, Vidhi Dhagia, Yu Han, Bing Guo, Mary E. Wines-Samuelson, Christine K. Christie, Qiangzong Yin, Orazio J. Slivano, Paul Herring, Xiaochun Long, Sachin A. Gupte, Joseph M. Miano

NYMC Faculty Publications

OBJECTIVE: Unreliable antibodies often hinder the accurate detection of an endogenous protein, and this is particularly true for the cardiac and smooth muscle cofactor, MYOCD (myocardin). Accordingly, the mouse Myocd locus was targeted with 2 independent epitope tags for the unambiguous expression, localization, and activity of MYOCD protein. APPROACH AND RESULTS: 3cCRISPR (3-component clustered regularly interspaced short palindromic repeat) was used to engineer a carboxyl-terminal 3xFLAG or 3xHA epitope tag in mouse embryos. Western blotting with antibodies to each tag revealed a protein product of approximately 150 kDa, a size considerably larger than that reported in virtually all publications. MYOCD …

Evaluation Of A Competency-Based Health Policy Training Program, Jim P. Stimpson, Kathleen Brandert, Brandon Grimm, Fernando A. Wilson

Reports: Center for Health Policy

An academic and practice partnership was formed to create and implement a competency-based training program for local health departments in health policy. We evaluated if the training program improved the policy knowledge and competency of participants.

Participants exhibited significant increases for self-assessed policy competency, including substantial improvements in “Critique the feasibility and expected outcomes of potential policy options”, “Identify and assess the strengths and motivations of key stakeholders and potential resistors”, and “Recommend a specific policy change”.

The policy competency instrument developed in this report could be used to measure policy knowledge and competency in future training implementations.

Pathogenicity And Protection Mediated By A Single Tcrβ In Experimental Autoimmune Encephalomyelitis, Tianhua Wu

Theses and Dissertations (ETD)

How the TCR repertoire, together with risk-associated major histocompatibility complex (MHC), imposes susceptibility for autoimmune disease is not fully understood. A small fraction of TCR α or β chains are “public”, and are shared by most individuals.High-throughput sequencing of the mouse TCRβ repertoire during myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmuneencephalomyelitis (EAE) identified a public TCRβ chain, TCRβ1, which was highly shared among individual mice and preferentially deployed during EAE. Retrogenic expression of TCRβ1 resulted in spontaneous early-onset EAE in mice with high penetrance and severity, despite being paired with a diverse endogenous TCRα repertoire. To further study autoimmunity conferred by …

Aging Suppresses Skin-Derived Circulating Sdf1 To Promote Full-Thickness Tissue Regeneration, Mailyn A. Nishiguchi, Casey A. Spencer, Denis H. Y. Leung, Thomas H. Leung

Research Collection School Of Economics

Physicians have observed that surgical wounds in the elderly heal with thinner scars than wounds in young patients. Understanding this phenomenon may reveal strategies for promoting scarless wound repair. We show that full-thickness skin wounds in aged but not young mice fully regenerate. Exposure of aged animals to blood from young mice by parabiosis counteracts this regenerative capacity. The secreted factor, stromal-derived factor 1 (SDF1), is expressed at higher levels in wounded skin of young mice. Genetic deletion of SDF1 in young skin enhanced tissue regeneration. In aged mice, enhancer of zeste homolog 2 (EZH2) and histone H3 lysine 27 …

Propofol Inhibits Prokaryotic Voltage-Gated Na+ Channels By Promoting Activation-Coupled Inactivation, Elaine Yang, Daniele Granata, Roderic G. Eckenhoff, Vincenzo Carnevale, Manuel Covarrubias

Department of Neuroscience Faculty Papers

Propofol is widely used in the clinic for the induction and maintenance of general anesthesia. As with most general anesthetics, however, our understanding of its mechanism of action remains incomplete. Local and general anesthetics largely inhibit voltage-gated Na⁺ channels (Navs) by inducing an apparent stabilization of the inactivated state, associated in some instances with pore block. To determine the biophysical and molecular basis of propofol action in Navs, we investigated NaChBac and NavMs, two prokaryotic Navs with distinct voltage dependencies and gating kinetics, by whole-cell patch clamp electrophysiology in the absence and presence of propofol at clinically relevant concentrations …

Race Analysis In An African American Majority Sample Of Individuals With Serious Mental Illness And Comorbid Diabetes Participating In A Self-Management Research Treatment Trail, Martha Sajatovic, Molly Howland, Douglas Gunzler, Kristin A. Cassidy, Richard Mccormick, Thomas Scheidemantel, Charles Thomas, Carol Blixen, Neal V. Dawson

Faculty Scholarship

Objectives: Targeted Training in Illness Management (TTIM) focuses on enhancing care engagement for people living with serious mental illness and diabetes. This secondary analysis from a 60-week, randomized controlled trial of TTIM versus treatment as usual evaluated racial subgroup outcomes. Method: Demographics, clinical characteristics, and diabetes status were evaluated for those self-identifying as non-Hispanic White, African American, and Hispanic. Longitudinal response to TTIM was evaluated using a multiple domain risk index. Due to their small sample size; those identifying as Hispanic were excluded from this analysis. Results: Non-Hispanic White participants had greater baseline socioeconomic advantages. Baseline risk scores, glycosylated hemoglobin …

Procarbazine, Ccnu And Vincristine (Pcv) Versus Temozolomide Chemotherapy For Patients With Low-Grade Glioma: A Systematic Review., Karim Hafazalla, Arjun Sahgal, Blessing Jaja, James R Perry, Sunit Das

Student Papers, Posters & Projects

Low-grade gliomas (LGG) encompass a heterogeneous group of tumors that are clinically, histologically and molecularly diverse. Treatment decisions for patients with LGG are directed toward improving upon the natural history while limiting treatment-associated toxiceffects. Recent evidence has documented a utility for adjuvant chemotherapy with procarbazine, CCNU (lomustine), and vincristine (PCV) or temozolomide (TMZ). We sought to determine the comparative utility of PCV and TMZ for patients with LGG, particularly in context of molecular subtype. A literature search of PubMed was conducted to identify studies reporting patient response to PCV, TMZ, or a combination of chemotherapy and radiation therapy (RT). Eligibility …

Occupational Therapy’S Role In Early Language Development Of Babies And Young Children, Aimee E. Ketchum, Otd, Otr/L, Marie-Christine Potvin, Phd, Otr/L

Student Papers, Posters & Projects

Occupational therapy practitioners in their interactions with babies, young children and their families, can be change-agents who promote rich language environments as a foundation to social interactions, social participation and academic successes. OTPs may be the sole provider working with a family or may reinforce with families the plan of care developed by speech-language pathologists (SLP) or early intervention teachers. The first step for OTPs is deeper knowledge of the importance of early language exposure, social interaction and participation development in young children and approaches that promotes rich language environments.

Ultrastructural Changes In Peripheral Blood Leukocytes In Α-Synuclein Knockout Mice, Hammad Tashkandi, Afshin Shameli, Clifford V. Harding, Robert W. Maitta

Faculty Scholarship

Effects of α-synuclein deficiency on cellular blood components have not been extensively investigated. This study evaluated ultrastructural changes of leukocytes in α-synuclein knockout (KO) mice using electron microscopy (EM). The following ultrastructural characteristics were quantified in leukocytes: mitochondria, primary granules, specific granules (SG), Golgi apparatus (GA), inclusions, rough-endoplasmic reticulum (RER), smooth-endoplasmic reticulum (SER), and cellular projections (CP). EM showed increased numbers or amounts of SG, inclusions, and SER in KO group (5.3 ± 4.5 in WT vs. 14.1 ± 10.3 in KO, p = 0.02; 0.4 ± 0.9 in WT vs. 3.2 ± 2.8 in KO, p = 0.007; and …