Medicine and Health Sciences Commons^™

Redox Regulation Of Type-I Inositol Trisphosphate Receptors In Intact Mammalian Cells., Suresh K. Joseph, Michael P. Young, Kamil Alzayady, David I. Yule, Mehboob Ali, David M. Booth, György Hajnóczky

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

A sensitization of inositol 1,4,5-trisphosphate receptor (IP3R)-mediated Ca2+ release is associated with oxidative stress in multiple cell types. These effects are thought to be mediated by alterations in the redox state of critical thiols in the IP3R, but this has not been directly demonstrated in intact cells. Here, we utilized a combination of gel-shift assays with MPEG-maleimides and LC-MS/MS to monitor the redox state of recombinant IP3R1 expressed in HEK293 cells. We found that under basal conditions, ∼5 of the 60 cysteines are oxidized in IP3R1. Cell treatment with 50 μm thimerosal altered gel shifts, indicating oxidation of ∼20 cysteines. …

Modeling In The Physiology Classroom, Sowmya Anjur

Faculty Publications & Research

Physiology and Disease is a Biology elective at IMSA that has been developed to be mostly student-centered. Some examples of student projects include modeling heart structure to reflect function and creating LED arduino monitors to measure heart rate. Students also measure their lung capacity and blood pressure to demonstrate correlation of these values with heart rate, and trace the correlation back to neuronal controls. Projects such as these integrate other disciplines such as engineering and conform to NGSS Science and Engineering standards and NGSS Cross cutting Concepts standards. Students take responsibility for their own learning and articulate better on tests.

The Santa Clara, 2018-11-08, Santa Clara University

The Santa Clara

No abstract provided.

In Vivo Extracellular Ph Mapping Of Tumors Using Electron Paramagnetic Resonance, Denis A. Komarov, Yuki Ichikawa, Kumiko Yamamoto, Neil J. Stewart, Shingo Matsumoto, Hironobu Yasui, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata

Clinical and Translational Science Institute

An electron paramagnetic resonance (EPR)- based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial +1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information on the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse …

Nutrition Risk Among An Ethnically Diverse Sample Of Community-Dwelling Older Adults, Patricia M. Sheean, Isabel C. Farrar, Suela Sulo, Jamie Partridge, Linda Schiffer, Marian Fitzgibbon

Nursing: School of Nursing Faculty Publications and Other Works

Objective

To assess the prevalence of nutritional risk among an ethnically diverse group of urban community-dwelling older adults and to explore if risk varied by race/ethnicity.

Design

Demographic characteristics, Katz’s activities of daily living and health-care resource utilization were ascertained cross-sectionally via telephone surveys with trained interviewers. Nutrition risk and nutrition symptomology were assessed via the abridged Patient Generated Subjective Global Assessment (abPG-SGA); scores of ≥6 points delineated ‘high’ nutrition risk. Descriptive statistics and logistic regression analyses were conducted.

Setting

Urban.

Participants

White, Black or Hispanic community-dwelling adults, ≥55 years of age, fluent in English or Spanish, residing in the …

Effects Of Inter-Stimulus Interval On Speech-Evoked Frequency-Following Response In Elderly Adults, Dongxin Liu, Jiong Hu, Ruijuan Dong, Jing Chen, Gabriella Musacchia, Shuo Wang

All Faculty Scholarship

Background: The speech-evoked frequency following response (FFR) has shown to be useful in assessing complex auditory processing abilities and in different age groups. While many aspects of FFR have been studied extensively, the effect of timing, as measured by inter-stimulus-interval (ISI), especially in the older adult population, has yet to be thoroughly investigated. Objective: The purpose of this study was to examine the effects of different ISIs on speech evoked FFR in older and younger adults who speak a tonal language, and to investigate whether the older adults’ FFR were more susceptible to the change in ISI. Materials and Methods: …

Winds Of Change – Predicting Water-Based Recreationists' Support And Opposition For Offshore Wind Energy Development In The Great Lakes, Michael D. Ferguson, Samantha L. Powers, Nate E. Trauntvein, Jeffrey B. Jacquet, Alan R. Graefe, Andrew J. Mowen

Kinesiology and Health Science Faculty Publications

This study examined the factors influencing water-based recreationists' perceptions of support and opposition towards off-shore wind energy development (OWD) on Lake Erie. Much of the proposed or future Lake Erie OWD infrastructure may either be within or adjacent to public lands, waters, and protected areas, raising concerns about the potential environmental and social impacts upon recreation stakeholders. The limited body of OWD research within the United States has suggested there are numerous factors that may influence overall perceptions of support and opposition such as political orientation and beliefs in climate change. Moreover, recent research has proposed that the perceived recreation …

Challenging Hazards Amidst Observational Simulation In The Emergency Department: Advancing Gamification In Simulation Education Through A Novel Resident-Led Skills Competition., Nicholas Salerno, Dimitrios Papanagnou, Md, Priyha Mahesh, Kaitlin M Bowers, Scott H Pasichow, Sara Paradise, Xiao Chi Zhang

Department of Emergency Medicine Faculty Papers

Medical simulation competitions have become an increasingly popular method to provide a hands-on "gamified" approach to education and training in the health professions. The most well-known competition, SimWars, consists of well-coordinated teams that are tasked with completing a series of mind-bending clinical scenarios in front of a live audience through 'bracket-style' elimination rounds. Similarly, challenging hazards amidst observational simulation (CHAOS) in the emergency department (ED) is another novel approach to gamification in both its structure and feel. Conducted at the Council of Emergency Medicine Resident Directors (CORD) 2018 National Assembly in San Antonio, Texas, instead of assigning premeditated teams, it …

I Was Clear With My Goals, Where I’M Heading, And What I Wanted With My Life: Life History Of An Omani Woman And Nurse Leader, Christie Emerson

Doctorate of Nursing Science Dissertations

This study uses life history methodology to examine the personal experience of being an Omani woman during the Omani Renaissance, choosing a career in nursing, and evolving into a nurse leader. There is a lack of research on why Omani women choose to enter the workforce, choose nursing career paths, and what sustains them in the nursing workforce. There is also a lack of research on the path to Omani nurse leadership. The research question is: What is the personal experience of being an Omani woman during the Omani Renaissance, choosing a career in nursing, and becoming a nurse leader? …

Faith Community Nursing In Community/Public Health Education: A Positive Student Nursing Experience, Pamela Preston

International Journal of Faith Community Nursing

Faith Community Nursing sites were recently integrated into a Community/Public Health Nursing course at a traditional Catholic baccalaureate college in New England. Junior and senior nursing students were randomly assigned to spend 10 clinical days at a site for their clinical experience. The students planned, participated in, and conducted diverse nursing and educational activities. At the end of the rotation, students completed evaluations rating the experience. The evaluations indicated that the clinical experience was positive. The diverse activities including support, health promotion, screening, and education in addition to the spiritual dimension allowed the students to participate in true holistic nursing …

Practice Matters: Screening For Upper Body Musculoskeletal Disorders In Faith Based Communities, Karen E. Furgal, Dawn M. Garrett Wright, Beth Norris

International Journal of Faith Community Nursing

Rural and faith farming communities engage in occupations that may make members susceptible to Musculoskeletal Disorders (MSD). Faith community nurses are well positioned to serve as first-line screeners when these injuries are identified. Thus, comprehensive screening methods and initial conservative management strategies in early stages of MSDs may be enough to prevent the need for more invasive care. Identifying those cases in which referral to another healthcare provider will also help management of MSDs in these communities.

A New Standard Dna Damage (Sdd) Data Format, J. Schuemann, A. L. Mcnamara, J. W. Warmenhoven, N. T. Henthorn, K. J. Kirkby, M. J. Merchant, S. Ingram, H. Paganetti, K. D. Held, J. Ramos-Mendez, B. Faddegon, J. Perl, D. T. Goodhead, I. Plante, H. Rabus, H. Nettelbeck, W. Friedland, P. Kundrát, A. Ottolenghi, G. Baiocco, S. Barbieri, M. Dingfelder, S. Incerti, C. Villagrasa, M. Bueno, M. A. Bernal, S. Guatelli, D. Sakata, J. M. C. Brown, Z. Francis, I. Kyriakou

Health Physics and Diagnostic Sciences Faculty Research

Our understanding of radiation-induced cellular damage has greatly improved over the past few decades. Despite this progress, there are still many obstacles to fully understand how radiation interacts with biologically relevant cellular components, such as DNA, to cause observable end points such as cell killing. Damage in DNA is identified as a major route of cell killing. One hurdle when modeling biological effects is the difficulty in directly comparing results generated by members of different research groups. Multiple Monte Carlo codes have been developed to simulate damage induction at the DNA scale, while at the same time various groups have …

Challenging Hazards Amidst Observational Simulation In The Emergency Department: Advancing Gamification In Simulation Education Through A Novel Resident-Led Skills Competition., Nicholas Salerno, Dimitrios Papanagnou, Priyha Mahesh, Kaitlin M Bowers, Scott H Pasichow, Sara Paradise, Xiao Chi Zhang

Medical Education Articles

Medical simulation competitions have become an increasingly popular method to provide a hands-on "gamified" approach to education and training in the health professions. The most well-known competition, SimWars, consists of well-coordinated teams that are tasked with completing a series of mind-bending clinical scenarios in front of a live audience through 'bracket-style' elimination rounds. Similarly, challenging hazards amidst observational simulation (CHAOS) in the emergency department (ED) is another novel approach to gamification in both its structure and feel. Conducted at the Council of Emergency Medicine Resident Directors (CORD) 2018 National Assembly in San Antonio, Texas, instead of assigning premeditated teams, it …

Mutant Ubqln2p497h In Motor Neurons Leads To Als-Like Phenotypes And Defective Autophagy In Rats, Tianhong Chen, Bo Huang, Xinglong Shi, Limo Gao, Cao Huang

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Mutations in ubiquilin2 (UBQLN2) have been linked to abnormal protein aggregation in amyotrophic lateral sclerosis (ALS). The mechanisms underlying UBQLN2-related neurodegenerative diseases remain unclear. Using a tetracycline-regulated gene expression system, the ALS-linked UBQLN2P497H mutant was selectively expressed in either the spinal motor neurons or astrocytes in rats. We found that selectively expressing mutant UBQLN2P497H in the spinal motor neurons caused several core features of ALS, including the progressive degeneration of motor neurons, the denervation atrophy of skeletal muscles, and the abnormal protein accumulation. Furthermore, mutant UBQLN2P497H accumulation was associated with an age-dependent decrease in several core autophagy-related proteins. ALS-like phenotypes …

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater

Posters-at-the-Capitol

Immunotherapy strategies are very promising treatments for cancer patients. Specifically, Immune checkpoint inhibitor therapy focusing on the PD-1/PD-L1 pathway shows long-lasting positive results in many cancer patients. Unfortunately, not all the patients can benefit from this highly effective treatment. Hence, there is a great need for predictive biomarkers. Immunohistochemical (IHC) staining has been used as a way of predicting patient response, yet shows many problems. For example, IHC utilizes an invasive biopsy and sample fixing, which creates an incomplete and delayed picture of the patient’s biochemistry and the tumor microenvironment, consequently ignoring metastases.

The purpose of this study is to …

Improving The Palatability Of Colonoscopy Preparations, Phuong Anh Hoang Nguyen, Darnell Leon Cuylear, Heather Elizabeth Canavan

Shared Knowledge Conference

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the United States. The most reliable screening method of CRC is a colonoscopy which requires a 4-Liter polymer with electrolytes preparation. Two in five patients are non-compliant to their colonoscopy schedules, with many patients who abstain reporting refusal due to significant discomfort associated with this preparation. Furthermore, there are distinct gender differences in the tolerance of colonoscopy preparations in male and female populations. We hypothesize the differences in clinic are a result of toxic effects of the drug associated with poor mixing by individual patients. PEG, the drug, …

An Engineer’S Take On The Bone-Ligament Interface: Utilizing Novel Technology To Improve Clinical Outcomes, Emma Garcia

Shared Knowledge Conference

Ligament repair is a common surgical practice with a significant lack of viable replacements. The current gold standard for repair is the use of tendon grafts from cadavers or from another place in the patient’s body; however, these often cause more problems than they solve including immune responses or a lack of mobility in another place in the body. Synthetic replacements are of growing interest, though the ability to mimic the complex structure of the ligament and how it connects to the bone remains an obstacle. Our lab built a 3D bioprinter combined with an electrospinner to address this complicated …

Computational Investigation Of The Interactions Between Bioactive Compounds And Biological Assemblies, Tye D. Martin

Shared Knowledge Conference

Design of small molecules is an ongoing focus for developing agents against pathogenic viruses and bacteria that are threats to worldwide health. Viruses such as Zika feature assemblies of repeat peptide subunits or capsid proteins which are potential targets for antiviral compounds. Other protein assemblies are implicated in pathology of Alzheimer’s Disease (AD) and additional neurodegenerative diseases characterized by large assemblies of misfolded proteins such as amyloid-beta (Aβ) and tau. Recent studies on a class of conjugated polyelectrolytes (CPEs) with phenylene ethynylene moieties and charged functional groups have shown potential both as bioactive antimicrobials and theragnostic sensing agents for tracking …

Targeted Exercise Therapy To Enhance Neural Activation, Terence Moriarty, Kelsey Bourbeau, Bryanne Bellovary, Micah Zuhl

Shared Knowledge Conference

Various types of exercise therapies, have been implemented into treatment for those suffering from psychological disorders and traumatic brain injury. The prefrontal cortex (PFC), which houses key cognitive constructs is responsive to exercise, and is commonly measured using functional near infrared spectroscopy (fNIRS). Evidence suggests that exercise mediates neural adaptation through increased blood flow and neurogenesis, which enhances neural activation leading to improved cognitive performance. However, the intensity of exercise that has the most robust impact on brain blood flow is currently unknown. Purpose. Therefore, the primary aim of the study is to compare PFC activation during cognitive tasks performed …

Gender Differences In First Authors, Peer Reviewers, And Grand Rounds Presenters In Medicine, Parisa Mortaji

Shared Knowledge Conference

Women are underrepresented in senior academic positions that depend on accomplishments like presentations and publications, and gender bias has shown to be a factor. We studied whether gender differences existed in multiple internal medicine venues: first authorship in a national trainee poster competition; first authorship in a national specialty-specific poster competition; peer reviewer-ship in three prestigious medical journals; and in presentations at UNM grand rounds. We found some progress in gender parity overall, but not yet enough. We first studied gender differences in authorship among residents in a 2015 national internal medicine resident poster competition; we found more authors were …

Effectiveness Of Therapy Interventions For Heterotopic Ossification About The Elbow: A Systematic Review, Cole Burns

Shared Knowledge Conference

Heterotopic ossification (HO) is the presence of bone where bone normally does not exist. HO forms frequently at the elbow from various conditions including: Fracture, orthopedic surgery, certain genetic disorders, neurological injuries, amputation, and brain injury. A diagnosis of HO at the elbow can lead to decreases in range of motion which effects the person’s functional independence. Surgical excision to remove the bone can be done to improve functional outcomes, but is typically not performed until a year has passed from the time of injury. Even then, there have been instances where people have experienced recurrent HO post-surgery. Because of …

Trying To Cover The Sun With Your Thumb: A Critical Ethnography Of Maternity Care Provision In Rural Northern New Mexico, Abigail Reese

Shared Knowledge Conference

Access to maternity care is disappearing for women across rural America. In New Mexico, women often travel long distances in order to access hospitals and providers that offer childbirth services, as these resources are primarily concentrated in metropolitan areas. Although data on provider distribution is available, very few studies have explored the maternity care access crisis from the perspectives of the midwives and physicians who work in rural areas. The purpose of this study was to explore barriers and facilitators to the provision of childbirth services from providers’ perspectives with the intent of informing policy debates around the maintenance of …

3d Bioprinting And Near-Field Electrospinning Composite Scaffolds For The Bone-Ligament Interface, Emma Garcia, Christina Salas, Matthew N. Rush, Christopher Buksa, Marissa Perez, Ava Mauser, Steven Nery, Fermin Prieto, Darielys Morales

Shared Knowledge Conference

3D bioprinting is an additive manufacturing technique that can utilize a range of bioactive materials to construct specific architectures that mimic native tissue. Near-field electrospinning (NFE) offers precise alignment control to create non-woven mats with high tensile strengths. We built a custom E-spin printer that enables layer-by-layer alternating deposition between 3D bioprinting and NFE to create composite scaffolds for the bone-ligament interface. This complex region is difficult to simulate due to its functionally graded mechanical and biochemical properties. We created NFE poly(caprolactone) highly aligned micro-fibers which formed collagen fibril-like bundles. Poly(ethylene glycol) diacrylate with decellularized bone was encased in the …

Early Interventions Providers’ Knowledge Of Evidence-Based Practices For Working With Children With Autism, Mariah Groll

Shared Knowledge Conference

Autism spectrum disorder (ASD) is developmental disability that is associated with social communication deficits and repetitive or restrictive behaviors. Research has systematically identified evidence-based strategies for working with individuals with ASD. It has demonstrated the importance of early intervention. There is, however, limited research on early intervention providers’ (e.g., speech-language pathologists (SLPs) and developmental specialists (DS)) knowledge of evidence-based strategies for working with individuals with ASD. To further explore this issue, we conducted an online survey that examined the ASD knowledge of early intervention DSs and SLPs in the state of NM. The survey included a demographics section, a section …

The Association Between Negative Socioeconomic Determinants Of Health And Poor Nutritional Status In Children Of Low Income Families Living In New Mexico, Molly A. Morrison

Shared Knowledge Conference

When evaluating the prevalence of obesity rates throughout New Mexico (NM), it appears these rates have reached a plateau. A recent study conducted by the NM Department of Health (DOH) found that NM was among 19 other states that have had a first time decrease in child obesity rates among low income families. However, when examined deeper, when looking at geographical areas throughout NM, hidden geography of weight disparities become apparent. Although obesity rates may have decreased in some areas, they have increased in others. The purpose of this study is to effectively evaluate variability in weight trends between geographical …

Cost-Effectiveness Analysis Of Enzalutamide, Abiraterone Acetate Plus Prednisone, Cabazitaxel Plus Prednisone For The Treatment Of Visceral Metastatic Castration Resistant Prostate Cancer (Mcrpc) After Docetaxel Therapy, Yazan K. Barqawi

Shared Knowledge Conference

Background: Prostate cancer is the second leading cause of death after lung cancer among men in the US. The America Cancer Society predicts 164,690 new cases and 29,430 prostate cancer deaths in 2018. In 2014, nearly US $13.4 billion was spent on prostate cancer in the US and expected to reach US $15.4 billion by 2020. Objective: To conduct a cost-effective analysis of visceral mCRPC therapies post-docetaxel failure from a US healthcare payer perspective utilizing life-time horizon Markov model. These medications received highest National Comprehensive Cancer Network guideline recommendation to treat visceral mCRPC post-docetaxel failure. Methods: A pharmacoeconomic model was …

The Critical Need For Mental Health Education To Be Mandated In New Mexico's Public Schools, Bonnie L. Murphy

Shared Knowledge Conference

Based on a review of research and best practices in mental health awareness and skills, this inquiry project argues for state legislative policies that would require mental health awareness and skills in the K-12 curriculum. Mental health affects individual accomplishments in every stage of people’s lives beginning in early childhood and throughout the life cycle. Prevention and treatment of mental illness plays a key role in the ability of an individual to cope with loss and develop resiliency and perseverance in challenging times and to make better decisions that improve the individual’s life and the lives of those around them. …

Improving The Palatability Of Colonoscopy Preparations, Phuong Anh Hoang Nguyen, Sarah Mounho, Darnell Cuylear, Heather Canavan

Shared Knowledge Conference

Colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the United States. The most reliable screening method of CRC is a colonoscopy which requires a 4-Liter polymer with electrolytes preparation. Two in five patients are non-compliant to their colonoscopy schedules, with many patients who abstain reporting refusal due to significant discomfort associated with this preparation. Furthermore, there are distinct gender differences in the tolerance of colonoscopy preparations in male and female populations. We hypothesize the differences in clinic are a result of toxic effects of the drug associated with poor mixing by individual patients. PEG, the drug, …

L-Serine Reduces Reactive Oxygen Species Yield In Cisplatin Treated Zebrafish Utricles, Satya A. Moolani, Elvin Irihamye, Jerry D. Monroe, Michael E. Smith

Posters-at-the-Capitol

Cisplatin is a chemotherapy compound effective against a variety of cancers. However, it can act as an ototoxin and cause hearing loss by promoting reactive oxygen species (ROS) production in auditory tissues. The antioxidant amino acid, L-serine has been hypothesized to lower levels of cisplatin-mediated ROS. In this project, we investigated whether L-serine can reduce cisplatin-mediated ROS production in auditory tissue and potentially act as an otoprotectant during cisplatin chemotherapy. We used a zebrafish utricular tissue culture system and fluorescent ROS indicator dye to spectrophotometrically measure if L-serine could decrease reactive oxygen species levels in cisplatin-treated tissues. We found that …

Fatigue In Adults With Cerebral Palsy: A Three-Year Follow-Up Study., Irene L B Oude Lansink, Patrick G Mcphee, Laura Kristine Brunton, Jan Willem Gorter

Physical Therapy Publications

OBJECTIVES: To describe the course of fatigue over a 3-year follow-up period in adults with cerebral palsy and to investigate the association of known determinants of fatigue (i.e. demographic characteristics and/or body composition) with change in fatigue.

METHODS: Forty-one adults with cerebral palsy from a previous study of fatigue were invited to participate in a follow-up study. Twenty-three adults with cerebral palsy (Gross Motor Function Classification System (GMFCS) levels I-V; mean age 38 years 2 months, standard deviation (SD) 14 years 1 month)) agreed to participate (convenience sample). Fatigue was measured with the Fatigue Impact and Severity Self-Assessment (FISSA, range …