Medicine and Health Sciences Commons^™

Sub-Saharan Africa’S “Other Diseases”: A Comparison Of The Global Response To Neglected Tropical Diseases And Hiv/Aids In Ghana, Rebecca Brosch

Political Science: Student Scholarship & Creative Works

Neglected tropical diseases (NTDs) refer to a group of diseases that affect 1.4 billion people worldwide, most commonly in low and low-middle income nations. These conditions can cause disabling and disfiguring effects that keep those inflicted from escaping poverty, leading to increased economic losses in already impoverished areas in Ghana and the rest of Sub-Saharan Africa. Although NTDs affect significantly more of the Ghanaian population, more government resources are given to HIV/AIDS (which only affects an estimated 1.5% of citizens). This research identifies the current response to NTDs (including government policy and NGO support) as well as how more global …

The Effects Of Allergies And Anaphylaxis On The Body And Mind: A Survey Of Opinions And Knowledge On These Disorders, Kathryn E. O'Brien

Honors Program: Student Scholarship & Creative Works

Despite common knowledge, allergies and anaphylaxis are considered disabilities. As such, these disorders affect the daily lives of those who have them, including one's mental health. In this paper, all the nuances of allergies and anaphylaxis will be discussed as the etiology and scientific aspects of allergies, anaphylaxis, and hypersensitivity are distinguished from one another. Furthermore, the findings are reported from two surveys; an assessment of how well educated and prepared teachers are for students with allergies and whether other students with food allergies have had similar experiences to the author's and those described in research by other studies concerning …

Driving In Early-Stage Alzheimer’S Disease: An Integrative Review Of The Literature, Rebecca L. Davis, Jennifer M. Ohman

Peer Reviewed Articles

One of the most difficult decisions for individuals with Alzheimer’s disease (AD) is when to stop driving. Because driving is a fundamental activity linked to socialization, independent functioning, and wellbeing, making the decision to stop driving is not easy. Cognitive decline in older adults can lead to getting lost while driving, difficulty detecting and avoiding hazards, as well as increased errors while driving due to compromised judgment and difficulty in making decisions. The purpose of the current literature review was to synthesize evidence regarding how individuals with early-stage AD, their families, and providers make determinations about driving safety, interventions to …

Exome Screening To Identify Loss-Of-Function Mutations In The Rhesus Macaque For Development Of Preclinical Models Of Human Disease, Adam Cornish, Robert M. Gibbs, Robert B. Norgren

Journal Articles: Genetics, Cell Biology & Anatomy

BACKGROUND: Exome sequencing has been utilized to identify genetic variants associated with disease in humans. Identification of loss-of-function mutations with exome sequencing in rhesus macaques (Macaca mulatta) could lead to valuable animal models of genetic disease. Attempts have been made to identify variants in rhesus macaques by aligning exome data against the rheMac2 draft genome. However, such efforts have been impaired due to the incompleteness and annotation errors associated with rheMac2. We wished to determine whether aligning exome reads against our new, improved rhesus genome, MacaM, could be used to identify high impact, loss-of-function mutations in rhesus macaques that would …

Analysis Of Point Of Sale Tobacco Retail Practices In Spokane, Ghazal Meratnia, Harry Daniels-Schatz, Kurtis Godfrey, Lauren Luther, Alexandra Norris, Mohamed Ahmed, Cerstan Bratlien, Hayley Hodgins

2016 Symposium

Tobacco is a leading cause of morbidity and mortality responsible for millions of deaths every year. According to the World Health Organization tobacco kills an estimated 6 million people every year. In 2011 in Washington State, 17.5% adults smoked (7th in the U.S.). Based on our survey results, the average price that Washington smokers reported paying for their last pack of cigarettes was $6.44 in 2009–2010; the range among states was $7.98 to $4.04. 2013 productivity loss in the U.S. exceeded $150 billion Tobacco is an important public health problem worldwide. Using the Standardized Tobacco Assessment for Retail Settings (STARS) …

Bone Health Promotion For Youth: A Primary Care Intervention, Kay G. Wilson

Doctor of Nursing Practice Projects

Obese youth have high rates of vitamin D and calcium deficiencies and are at risk for developing osteoporosis. Clinical guidelines are needed in primary pediatric care services to promote bone-healthy behaviors. The aim of this project was to: a) implement the infrastructure and processes for physiological monitoring, consistent parameter-based vitamin D & calcium supplementation, family education, and coaching, and b) evaluate the impact of bone promotion changes on patients and parents and the primary care delivery system. The project outcomes included modest gains in bone health awareness among staff, patients, and families. In addition, modifications to clinical pathways and related …

Establishing A Nurse Mentor Program To Improve Nurse Satisfaction And Intent To Stay, Sara Jane Jones

Doctor of Nursing Practice Projects

Retention of new graduate registered nurses (RNs) is a problem within the healthcare system negatively impacting patient safety and health care outcomes. The problem of retention of qualified RNs is compounded by the potential shortage of RNs, the aging RN workforce and the aging US population. During a period of transition, a novice RN requires the guidance of others to learn to apply theoretical knowledge to real life clinical experiences. In the linear progression of Benner’s levels of clinical competency, the beginning two levels of nurses need a resource person to guide their progression in clinical practice. A mentoring relationship …

Community Acquired Pneumonia Project, Patricia Heaney

Doctor of Nursing Practice Projects

There is an increasing number of microorganisms that are becoming resistant to antimicrobial medications. This growing number of resistant organism poses a serious health threat, not only in this nation, but throughout the world. The Center for Disease Control and Prevention (CDC) estimates that there are greater than 2 million people who acquire serious infections that are resistant to one or more antimicrobial agents that were made to treat those organisms (CDC, 2013). Streptococcus pneumonia (S. pneumoniae or pneumococcus) is one such organism that has been identified by the CDC (2013) as becoming increasingly resistant to drugs that were once …

Follow-Up Telephone Contact Following Discharge From Long-Term Acute Care Hospitals, Tonja Williams

Doctor of Nursing Practice Projects

Readmissions to acute care hospitals within 30 days post discharge are a burden to healthcare economy. Medicare Payment Advisory Commission (2007) estimated a cost of $12 billion dollars is spent each year on Medicare patients who are readmitted to an acute care hospital within 30 days post discharge. MedPAC (2007) estimated that 75% of those readmissions are avoidable. A review of the literature was conducted. Thirteen studies were reviewed and analyzed and the results of the literature review indicated there is evidence supportive of implementing telephone follow-up contact with patients discharged from the LTACH to assess for additional needs and …

Implementing An Intervention To Reduce Seclusion And Restraints In An Inpatient Psychiatric Hospital, Amanda B. Lykins

Doctor of Nursing Practice Projects

The need to enhance nursing staff’s knowledge, perceptions and attitudes around alternatives to seclusion and restraint has been well validated in the literature. Seclusion and restraint use in the inpatient psychiatric setting are highly dangerous physical interventions, used to control the behavior of a mentally ill patient who is a danger to self or others. Seclusion and restraint events have the potential to cause emotional trauma, physical injury to patients or staff and the possibility of patient death. The purpose of the capstone project was to implement an evidence-based education program to, improve nursing staff’s (RN, LPN, MHA and SRNA) …

Microvascular Endothelial Cells-Derived Microvesicles Imply In Ischemic Stroke By Modulating Astrocyte And Blood Brain Barrier Function And Cerebral Blood Flow, Qunwen Pan, Caixia He, Hua Liu, Xiaorong Liao, Bingyan Dai, Yanfang Chen, Yi Yang, Bin Zhao, Ji C. Bihl, Xiaotang Ma

Pharmacology and Toxicology Faculty Publications

Background

Endothelial cell (EC) released microvesicles (EMVs) can affect various target cells by transferring carried genetic information. Astrocytes are the main components of the blood brain barrier (BBB) structure in the brain and participate in regulating BBB integrity and blood flow. The interactions between ECs and astrocytes are essential for BBB integrity in homeostasis and pathological conditions. Here, we studied the effects of human brain microvascular ECs released EMVs on astrocyte functions. Additionally, we investigated the effects of EMVs treated astrocytes on regulating BBB function and cerebral ischemic damage.

Results

EMVs prepared from ECs cultured in normal condition (n-EMVs) or …

Forging Fresh Food Chains In The Americas, Lisa B. Markowitz

The Chautauqua Journal

No abstract provided.

Management Of Charcot–Marie–Tooth Disease: Improving Long-Term Care With A Multidisciplinary Approach, Donald Mccorquodale, Evan M. Pucillo, Nicholas E. Johnson

Physical Therapy Collection

Charcot–Marie–Tooth (CMT) disease is the most common inherited neuropathy and one of the most common inherited diseases in humans. The diagnosis of CMT is traditionally made by the neurologic specialist, yet the optimal management of CMT patients includes genetic counselors, physical and occupational therapists, physiatrists, orthotists, mental health providers, and community resources. Rapidly developing genetic discoveries and novel gene discovery techniques continue to add a growing number of genetic subtypes of CMT. The first large clinical natural history and therapeutic trials have added to our knowledge of each CMT subtype and revealed how CMT impacts patient quality of life. In …

Perspectives On Teaching The International Classification Of Functioning, Disability, And Health Model To Physical Therapy Students, Cherie Peters-Brinkerhof

Physical Therapy Collection

BACKGROUND: During a re-accreditation visit, deficiencies were discovered in the clinical education curriculum regarding patient-centered care in a Doctorate of Physical Therapy program. To understand the problem and address those deficiencies, the clinical internship experience was examined using the International Classification of Functioning, Disability, and Health (ICF) model as a conceptual framework for clinical reasoning.

OBJECTIVE: This qualitative case study aimed to study (1) perceptions of physical therapy (PT) students regarding their knowledge and learning experiences during clinical affiliations and what knowledge they acquired of the ICF as applied to patient-centered care during their internship, and (2) the perceptions of …

Quantitative Comparisons On Hand Motor Functional Areas Determined By Resting State And Task Bold Fmri And Anatomical Mri For Pre-Surgical Planning Of Patients With Brain Tumors, Bob L. Hou, Sanjay Bhatia, Jeffrey S. Carpenter

Faculty & Staff Scholarship

For pre-surgical planning we present quantitative comparison of the location of the hand motor functional area determined by right hand finger tapping BOLD fMRI, resting state BOLD fMRI, and anatomically using high resolution T1 weighted images. Data were obtained on 10 healthy subjects and 25 patients with left sided brain tumors. Our results show that there are important differences in the locations (i.e.,N20 mm) of the determined hand motor voxels by these three MR imaging methods. This can have significant effect on the pre-surgical planning of these patients depending on the modality used. In 13 of the 25 cases (i.e., …

Use Of Selective-Serotonin Reuptake Inhibitors And Platelet Aggregation Inhibitors Among Individuals With Co-Occurring Atherosclerotic Cardiovascular Disease And Depression Or Anxiety, J. Douglas Thornton, Parul Agarwal, Usha Sambamoorthi

Faculty & Staff Scholarship

Objective: Medications commonly used to treat heart disease, anxiety, and depression can interact resulting in an increased risk of bleeding, warranting a cautious approach in medical decision making. This retrospective, descriptive study examined the prevalence and the factors associated with the use of both selective-serotonin reuptake inhibitor and platelet aggregation inhibitor among individuals with co-occurring atherosclerotic cardiovascular disease and anxiety or depression.

Methods: Respondents aged 22 years and older, alive throughout the study period, and diagnosed with co-occurring atherosclerotic cardiovascular disease and anxiety or depression (n=1507) in years 2007 through 2013 of the Medical Expenditures Panel Survey were included. The …

Non-Medical Use Of Cognitive Enhancing Prescription Medications Among Occupational Therapy And Speech Language Pathology Health Care Students: A Pilot Study, Randy P. Mccombie, Hannah Slanina

Faculty & Staff Scholarship

Purpose: This research was designed to serve as a pilot study to generate baseline data on non-medical use of prescription drugs (NMUPD) for cognitive/academic enhancement purposes among students representing two healthcare professions, Occupational Therapy (OT) and Speech Language Pathology (SLP) and to assess students’ opinions regarding whether use of these medications constitutes academic “cheating.” Introduction: NMUPD, such as Adderall ® or Ritalin ®, to reduce fatigue, improve memory, and increase concentration to ultimately improve grades has increased among college students in recent years, with estimated use put as high as 35% on some campuses. These drugs appear to be readily …

Assessing The Association Of Mitochondrial Genetic Variation With Primary Open-Angle Glaucoma Using Gene-Set Analyses, Anthony P. Khawaja, Jessica N. Cooke Bailey, Jae Hee Kang, R. Rand Allingham, Michael A. Hauser, Murray Brilliant, Donald L. Budenz, William G. Christen, John Fingert, Douglas Gaasterland, Terry Gaasterland, Peter Kraft, Richard K. Lee, Paul R. Lichter, Yutao Liu, Felipe Medeiros, Syoko E. Moroi, Julia E. Richards, Tony Realini, Robert Ritch, Joel S. Schuman, William K. Scott, Kuldev Singh, Arthur J. Sit, Douglas Vollrath, Gadi Wollstein, Donald J. Zack, Kang Zhang, Margaret Pericak-Vance, Robert N. Weinreb, Jonathan L. Haines, Louis R. Pasquale, Janey L. Wiggs

Faculty & Staff Scholarship

PURPOSE. Recent studies indicate that mitochondrial proteins may contribute to the pathogenesis of primary open-angle glaucoma (POAG). In this study, we examined the association between POAG and common variations in gene-encoding mitochondrial proteins. METHODS. We examined genetic data from 3430 POAG cases and 3108 controls derived from the combination of the GLAUGEN and NEIGHBOR studies. We constructed biological-system coherent mitochondrial nuclear-encoded protein gene-sets by intersecting the MitoCarta database with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. We examined the mitochondrial gene-sets for association with POAG and with normal-tension glaucoma (NTG) and high-tension glaucoma (HTG) subsets using Pathway Analysis …

Dual-Seq Transcriptomics Reveals The Battle For Iron During Pseudomonas Aeruginosa Acute Murine Pneumonia, F Heath Damron, Amanda G. Oglesby-Sherrouse, Angela Wilks, Mariette Barbier

Faculty & Staff Scholarship

Determining bacterial gene expression during infection is fundamental to understand pathogenesis. In this study, we used dual RNA-seq to simultaneously measure P. aeruginosa and the murine host’s gene expression and response to respiratory infection. Bacterial genes encoding products involved in metabolism and virulence were differentially expressed during infection and the type III and VI secretion systems were highly expressed in vivo. Strikingly, heme acquisition, ferric-enterobactin transport, and pyoverdine biosynthesis genes were found to be significantly up-regulated during infection. In the mouse, we profiled the acute immune response to P. aeruginosa and identified the pro-inflammatory cytokines involved in acute response …

Oral Perfluorooctane Sulfonate (Pfos) Lessens Tumor Development In The Apcmin Mouse Model Of Spontaneous Familial Adenomatous Polyposis, Jeffrey Wimsatt, Meghan Villers, Laurel Thomas, Stacey Kamarec, Caitlin Montgomery, Leo W. Y. Yeung, Yanqing Hu, Kim Innes

Faculty & Staff Scholarship

Colorectal cancer is the second most common cause of cancer deaths for both men and women, and the third most common cause of cancer in the U.S. Toxicity of current chemotherapeutic agents for colorectal cancer, and emergence of drug resistance underscore the need to develop new, potentially less toxic alternatives. Our recent cross-sectional study in a large Appalachian population, showed a strong, inverse, dose–response association of serum perfluorooctane sulfonate (PFOS) levels to prevalent colorectal cancer, suggesting PFOS may have therapeutic potential in the prevention and/or treatment of colorectal cancer. In these preliminary studies using a mouse model of familial colorectal …

Spatial And Temporal Variations Of Screening For Breast And Colorectal Cancer In The United States, 2008 To 2012, Xue Feng, Xi Tan, Ebtihag Alenzi, Pragya Rai, Jongwha Chang

Faculty & Staff Scholarship

Cancer screening tests are important tools to combat cancer-related morbidity and mortality. There is limited up-to-date research on spatial and temporal variations of colorectal and breast cancer screening in the United States.

County-level data of cancer screening adherence rates were generated from 2008 to 2012 Behavioral Risk Factor Surveillance System. We performed the univariate local indicators for spatial analyses (LISA) for the geographic differences of screening adherence rate and the differential LISA for the change of screening adherence rate from 2008 to 2012.

In the univariate LISA, low-to-low clusters were consistently identified in counties of New Mexico, Wyoming, and Mississippi …

Oxidative Stress Induces Transient O‐Glcnac Elevation And Tau Dephosphorylation In Sh‐Sy5y Cells, Emese Katai, Jozsef Pal, Viktor Soma Poor, Rupeena Purewal, Attila Miseta, Tamas Nagy

Faculty & Staff Scholarship

O‐linked β‐N‐acetlyglucosamine or O‐GlcNAc modification is a dynamic post‐translational modification occurring on the Ser/Thr residues of many intracellular proteins. The chronic imbalance between phosphorylation and O‐GlcNAc on tau protein is considered as one of the main hallmarks of Alzheimer's disease. In recent years, many studies also showed that O‐GlcNAc levels can elevate upon acute stress and suggested that this might facilitate cell survival. However, many consider chronic stress, including oxidative damage as a major risk factor in the development of the disease. In this study, using the neuronal cell line SH‐SY5Y we investigated the dynamic nature of O‐GlcNAc …

Return To Play After Cervical Spine Injuries: A Consensus Of Opinion, John C. France, Michael Karsy, James S. Harrop, Andrew T. Dailey

Faculty & Staff Scholarship

Study Design: Survey. Objective: Sports-related spinal cord injury (SCI) represents a growing proportion of total SCIs but lacks evidence or guidelines to guide clinical decision-making on return to play (RTP). Our objective is to offer the treating physician a consensus analysis of expert opinion regarding RTP that can be incorporated with the unique factors of a case for clinical decision-making. Methods: Ten common clinical scenarios involving neurapraxia and stenosis, atlantoaxial injury, subaxial injury, and general cervical spine injury were presented to 25 spine surgeons from level 1 trauma centers for whom spine trauma is a significant component of their practice. …

Capacity Fade Analysis Of Sulfur Cathodes In Lithium–Sulfur Batteries, Jianhua Yan, Xingbo Liu, Bingyun Li

Faculty & Staff Scholarship

Rechargeable lithium–sulfur (Li–S) batteries are receiving ever-increasing attention due to their high theoretical energy density and inexpensive raw sulfur materials. However, their rapid capacity fade has been one of the key barriers for their further improvement. It is well accepted that the major degradation mechanisms of S-cathodes include low electrical conductivity of S and sulfides, precipitation of nonconductive Li2S2 and Li2S, and poly-shuttle effects. To determine these degradation factors, a comprehensive study of sulfur cathodes with different amounts of electrolytes is presented here. A survey of the fundamentals of Li–S chemistry with respect to capacity fade is first conducted; then, …

Machine-Learning Approach Identifies A Pattern Of Gene Expression In Peripheral Blood That Can Accurately Detect Ischaemic Stroke, Grant C. O'Connell, Ashley B. Petrone, Madison B. Treadway, Connie S. Tennant, Noelle Lucke-Wold, Paul D. Chantler, Taura L. Barr

Faculty & Staff Scholarship

Early and accurate diagnosis of stroke improves the probability of positive outcome. The objective of this study was to identify a pattern of gene expression in peripheral blood that could potentially be optimised to expedite the diagnosis of acute ischaemic stroke (AIS). A discovery cohort was recruited consisting of 39 AIS patients and 24 neurologically asymptomatic controls. Peripheral blood was sampled at emergency department admission, and genome-wide expression profiling was performed via microarray. A machine-learning technique known as genetic algorithm k-nearest neighbours (GA/kNN) was then used to identify a pattern of gene expression that could optimally discriminate between groups. This …

Concept Of An Upright Wearable Positron Emission Tomography Imager In Humans, Christopher E. Bauer, Julie Brefczynski-Lewis, Gary Marano, Mary-Beth Mandich, Alexander Stolin, Peter Martone, James W. Lewis, Gangadhar Jaliparthi, Raymond R. Raylman, Stan Majewski

Faculty & Staff Scholarship

Background: Positron Emission Tomography (PET) is traditionally used to image patients in restrictive positions, with few devices allowing for upright, brain-dedicated imaging. Our team has explored the concept of wearable PET imagers which could provide functional brain imaging of freely moving subjects. To test feasibility and determine future considerations for development, we built a rudimentary proof-of-concept prototype (Helmet_PET) and conducted tests in phantoms and four human volunteers. Methods: Twelve Silicon Photomultiplier-based detectors were assembled in a ring with exterior weight support and an interior mechanism that could be adjustably fitted to the head. We conducted brain phantom tests as well …

An Ovarian Bioreactor For In Vitro Culture Of The Whole Bovine Ovary: A Preliminary Report, Matthew R. Zanotelli, Joseph D. Henningsen, Patrick M. Hopkins, Aaron P. Dederich, Tessa Herman, Tracy J. Puccinelli, Sana M. Salih

Faculty & Staff Scholarship

Background: Improved cancer therapeutics and enhanced cancer survivorship have emphasized the severe long-term side effects of chemotherapy. Specifically, studies have linked many chemotherapy agents with primary ovarian insufficiency, although an exact insult model has not yet been determined. To investigate and ultimately solve this problem, a novel device for extended study of mammalian ovaries in vitro was developed.

Methods: A bioreactor was fabricated for bovine ovarian culture that provides intravascular delivery of media to the ovary through isolation and cannulation of a main ovarian artery branch. Whole ovaries were cultured in vitro using three methods: (1) continuously supplied fresh culture …

Differential Pulmonary Effects Of Coo And La2o3 Metal Oxide Nanoparticle Responses During Aerosolized Inhalation In Mice, Jennifer D. Sisler, Ruibin Li, Walter Mckinney, Robert R. Mercer, Zhaoxia Ji, Tian Xia, Xiang Wang, Justine Shaffer, Marlene Orandle, Amy L. Mihalchik, Lori Battelli, Bean T. Chen, Michael Wolfarth, Michael E. Andrew, Diane Schwegler-Berry, Dale W. Porter, Vincent Castranova, Andre Nel, Yong Qian

Faculty & Staff Scholarship

Background: Although classified as metal oxides, cobalt monoxide (CoO) and lanthanum oxide (La2O3) nanoparticles, as representative transition and rare earth oxides, exhibit distinct material properties that may result in different hazardous potential in the lung. The current study was undertaken to compare the pulmonary effects of aerosolized whole body inhalation of these nanoparticles in mice.

Results: Mice were exposed to filtered air (control) and 10 or 30 mg/m3 of each particle type for 4 days and then examined at 1 h, 1, 7 and 56 days post-exposure. The whole lung burden 1 h after the 4 day inhalation of CoO …

Socioecological Factors Influencing Women’S Hiv Risk In The United States: Qualitative Findings From The Women’S Hiv Seroincidence Study (Hptn 064), Paula M. Frew, Kimberly Parker, Linda Vo, Danielle Haley, Ann O'Leary, Dazon Dixon Diallo, Carol E. Golin, Irene Kuo, Lydia Soto-Torres, Jing Wang, Adaora A. Adimora, Laura A. Randall, Carlos Del Rio, Sally Hodder

Faculty & Staff Scholarship

Background: We sought to understand the multilevel syndemic factors that are concurrently contributing to the HIV epidemic among women living in the US. We specifically examined community, network, dyadic, and individual factors to explain HIV vulnerability within a socioecological framework. Methods: We gathered qualitative data (120 interviews and 31 focus groups) from a subset of women ages 18–44 years (N = 2,099) enrolled in the HPTN 064 HIV seroincidence estimation study across 10 US communities. We analyzed data from 4 diverse locations: Atlanta, New York City (the Bronx), Raleigh, and Washington, DC. Data were thematically coded using grounded theory methodology. …

Effect Of Soluble Epoxide Hydrolase On The Modulation Of Coronary Reactive Hyperemia: Role Of Oxylipins And Pparγ, Ahmad Hanif, Matthew L. Edin, Darryl C. Zeldin, Christophe Morisseau, Mohammed A. Nayeem

Faculty & Staff Scholarship

Coronary reactive hyperemia (CRH) is a physiological response to ischemic insult that prevents the potential harm associated with an interruption of blood supply. The relationship between the pharmacologic inhibition of soluble epoxide hydrolase (sEH) and CRH response to a brief ischemia is not known. sEH is involved in the main catabolic pathway of epoxyeicosatrienoic acids (EETs), which are converted into dihydroxyeicosatrienoic acids (DHETs). EETs protect against ischemia/reperfusion injury and have numerous beneficial physiological effects. We hypothesized that inhibition of sEH by t-AUCB enhances CRH in isolated mouse hearts through changing the oxylipin profiles, including an increase in EETs/DHETs ratio. Compared …