Medicine and Health Sciences Commons^™

Multisite Phosphorylation Of The Sum1 Transcriptional Repressor By S-Phase Kinases Controls Exit From Meiotic Prophase In Yeast., Daniel Corbi, Sham Sunder, Michael Weinreich, Aikaterini Skokotas, Erica S Johnson, Edward Winter

Department of Biochemistry and Molecular Biology Faculty Papers

Activation of the meiotic transcription factor Ndt80 is a key regulatory transition in the life cycle of Saccharomyces cerevisiae because it triggers exit from pachytene and entry into meiosis. The NDT80 promoter is held inactive by a complex containing the DNA-binding protein Sum1 and the histone deacetylase Hst1. Meiosis-specific phosphorylation of Sum1 by the protein kinases Cdk1, Ime2, and Cdc7 is required for NDT80 expression. Here, we show that the S-phase-promoting cyclin Clb5 activates Cdk1 to phosphorylate most, and perhaps all, of the 11 minimal cyclin-dependent kinase (CDK) phospho-consensus sites (S/T-P) in Sum1. Nine of these sites can individually promote …

Improving Consistency Of Goal Attainment To Increase Physical Activity, Elizabeth Anne Solley

USF Tampa Graduate Theses and Dissertations

Researchers have successfully increased physical activity with self-monitoring, goal setting, and feedback. Goal attainment is a crucial part of what makes goal setting successful; however, it is often unreported in the literature or implied that goals were not reached consistently. A potential way to achieve this consistency is to create an action plan, or a detailed account of exactly how and when the individual will engage in the desired physical activity to reach his or her goal. This study evaluated whether making a detailed action plan would allow individuals to reach their physical activity goals more consistently than when using …

Hospital Staff Registered Nurses’ Perception Of Horizontal Violence, Peer Relationships, And The Quality And Safety Of Patient Care, Christina Purpora, M. A. Blegen, N. A. Stotts

Nursing and Health Professions Faculty Research and Publications

Objective: To test hypotheses from a horizontal violence and quality and safety of patient care model: horizontal violence (negative behavior among peers) is inversely related to peer relations, quality of care and it is positively related to errors and adverse events. Additionally, the association between horizontal violence, peer relations, quality of care, errors and adverse events, and nurse and work characteristics were determined.

Participants: A random sample (n=175) of hospital staff Registered Nurses working in California.

Methods: Nurses participated via survey. Bivariate and multivariate analyses tested the study hypotheses.

Results: Hypotheses were supported. Horizontal violence was inversely related to peer …

Designing Your Research Poster: Tips And Best Practices, Medical Media Services

Electronic Handouts

One page handout on designing your research poster including tips and best practices from Medical Media Services at Thomas Jefferson University.

Vaccination Status And Attitudes Of Urban School Employees In Utah, Kim Estella Thompson

Theses and Dissertations

Individuals who work with children are at risk for exposure to vaccine-preventable diseases. School settings can quickly become outbreak centers for communicable infection since school employees are in direct contact with children in confined areas for many hours each day. Therefore, it is important for school employees to be fully vaccinated. There are many reasons school employees may be inadequately vaccinated. One common myth is that adults believe vaccines are only for children. Another reason for inadequate vaccination rates among school employees is that many adults believe vaccinations received during childhood are still effective. Healthcare providers (HCPs) constitute the first …

A Brief History Of Public Health In Alexandria And Alexandria's Health Department, Krystyn R. Moon

History and American Studies Articles

One of the central tenets of public health is the belief that the practice of medicine serves the broader community; however, the specific meaning of the phrase “public health” is a historically contingent one. Beginning in the late eighteenth century, the public included only white, bourgeois men who met in coffee houses and other such establishments to discuss politics, business ventures, and popular culture. Over the course of the nineteenth and twentieth centuries, the “public” included white working class men, the poor, women, and people of color. Eventually, politicians and medical doctors recognized that children should be included as part …

Using Video Feedback To Increase Eye Contact During Mock Job Interviews For Transition Age Adults With Autism Spectrum Disorders, Alexia Barnes

USF Tampa Graduate Theses and Dissertations

Abstract

Autism Spectrum Disorders (ASD) are characterized by impairments in social and communicative behaviors. These impairments can impact an individual's ability to obtain employment. The rates of unemployment for individuals with ASD are much higher than those of their peers without ASD. This study used a multiple baseline design across three participants diagnosed with ASD to determine the effectiveness of video feedback in improving eye contact duration during mock job interviews. After video feedback sessions, eye contact duration increased across all participants. On average, participant's eye contact duration was at 30.2% during baseline. These percentages increased to an average of …

Practicing Patienthood Online: Social Media, Chronic Illness, And Lay Expertise, Collette Sosnowy

Publications and Research

The use of digital technologies and social media by people with serious illness to find, share, and create health information is much celebrated but rarely critiqued. Proponents laud “Health 2.0” as transforming health care practice and empowering patients. Critics, however, argue that a discourse of developing lay expertise online masks the disciplinary practices of the neoliberal state’s emphasis on individual responsibility. Notably, the perspectives of people who are engaging with social media related to their health and illness are under-represented in this debate. This research examines the experiences and perspectives of women who blog about their lives with Multiple Sclerosis …

Water And Health In The Nandamojo Watershed Of Costa Rica: Community Perceptions Towards Water, Sanitation, And The Environment, James Mcknight

USF Tampa Graduate Theses and Dissertations

Understanding the relationships between human health, water, sanitation, and environmental health is a requirement to understanding the challenges that face researchers when it comes to addressing global health relating to water and sanitation. Access to improved water and sanitation is not only a precondition to health, but to all aspects of daily living. Target 7.C of the Millennium Development Goals (MDGs) addresses worldwide disparities in access to improved water and sanitation by calling for the reduction in "half of the proportion of people without sustainable access to safe drinking water and sanitation by 2015". Over 90% of the population of …

Kids Alive! Support For Children Of Parents With Cancer: An Exemplar Program For Children Of Parents With Cancer, Shane Spears

Graduate School of Professional Psychology: Doctoral Papers and Masters Projects

Cancer in a parent or caregiver is an event that affects the whole family. The roles and responsibilities of the diagnosed parent, as well as those of each family member, are affected at the time of diagnosis and throughout the progression of the illness. According to the American Cancer Society, there will be an estimated 1,665,540 new cancer cases diagnosed and 585,720 cancer deaths in 2014. This staggering statistic means there are a number of cancer diagnoses that will directly affect thousands of parents and their children. Past research suggests this upheaval in the system is particularly stressful on children …

Development Of A Partnership For International Rural Advanced Pharmacy Practice Experiences, Emily K. Flores, Linsey A. Courtney

International Journal of Health Sciences Education

Objective. To design a faculty-guided international elective Advanced Pharmacy Practice Expereince (APPE) in partnership with a medical relief organization. To expose students to pharmacy-related opportunities in non-traditional settings focused on an indigent population, while obtaining a global perspective on healthcare.

Methods. The College of Pharmacy partnered with an international medical relief organization utilizing their resources for trip planning and in-country logistics to provide a framework for a pharmacy student rotation. The international trip is a portion of the calendar month rotation, while the balance is faculty-guided discussion groups, involvement in formulary planning, developing educational materials, and local medical relief work. …

Report Of A Curriculum Used In A Peer-Delivered Intervention To Reduce Obesity Of Adolescents In Southern Appalachia And Its Relationship To The National Health Education Standards, Diana Mozen, William Dalton, Taylor Mckeehan, Deborah Slawson

International Journal of Health Sciences Education

Adolescent obesity in Southern Appalachian is among the highest in the nation. Even though adolescent obesity is a major public health concern, effective interventions are limited. Team Up for Healthy Living is a cluster-randomized control trial developed to test the effectiveness of a cross-peer intervention with high school students focusing on healthy eating and physical activity. This instructional article describes the 8-week curriculum developed and utilized by Team Up for Healthy Living with an emphasis on the relationship to National Health Education Standards. This is important given the standards were created to promote and support health-enhancing behaviors for students all …

The Use Of High-Fidelity Simulation In Psychiatric And Mental Health Nursing Clinical Education, Bethany A. Murray

International Journal of Health Sciences Education

Background: High-fidelity simulation recreates real-life situations in a safe learning environment and encourages critical thinking in students. Published research in simulation in psychiatric/mental health nursing is sparse.

Methods: Four scenarios exemplifying drug or alcohol abuse utilizing the computerized, mannequin SimMan® were implemented. Students evaluated their learning experience following completion of the simulation via a 20-item, Likert-scale survey which included open-ended questions.

Results: Results were positive. Students rated all items on the survey as “agree” or “strongly agree” (Mean 4.77, SD=0.55).

Conclusions: High fidelity clinical education simulations are an effective means of facilitating student learning of psychiatric and mental health clinical …

A Coordinated School Health Approach To Obesity Prevention Among Appalachian Youth: Middle School Student Outcomes From The Winning With Wellness Project, William T. Dalton Iii, Karen Schetzina, Elizabeth Conway-Williams

International Journal of Health Sciences Education

The Winning With Wellness (WWW) project was a school-based obesity prevention program that was developed to promote healthy eating and physical activity in youth residing in rural Appalachia. The project was based on the Coordinated School Health model (Centers for Disease Control and Prevention (CDC), 2013a) and used a community-based participatory research approach with an emphasis on feasibility and sustainability. The purpose of this study was to examine self-reported health outcomes for middle school students across the course of the intervention. Sixth grade middle school students (N = 149; 52% girls) from four schools in Northeast Tennessee completed a …

Global Summation Of Radial Frequency Patterns And The Effect Of Sudden Onset Glare On Shape Discrimination, Edgar Ekure

Student Theses, Dissertations and Capstones

ABSTRACT Global summation of radial frequency patterns and the effect of sudden onset glare on shape discrimination The purpose of this study was to provide evidence of global pooling around the circumference of the Radial frequency (RF) pattern, and to study the effect of sudden onset glare on shape discrimination. The RF stimuli were generated by the amplitude modulation of the radius of a circle which deforms them from circularity, while the cross sectional luminance profile was the fourth derivative of Gaussians (D4). The amplitude of the stimuli determines how distinct the pattern is and thus measures the degree of …

Improving Access To Healthcare In New Orleans: An Up-To-Date Guide Of Free Or Low-Cost Clinics And Medication Assistance, Richard Brucker, Peggy Chehardy

Pediatrics Research and Scholarship

After Hurricane Katrina, New Orleans safety net, Charity Hospital, was closed. Numerous free or low-cost clinics opened around town. Yet homeless individuals still expressed a need for more free clinics, as they were unaware of the resources that already existed. In August 2007, the resources available were out-dated, inaccurate, and lacked information regarding cost of medical care. Upon verifying information, print guides were assembled and thousands of copies were dispersed to homeless individuals. A website, with both online and print guides, was created to address the difficult task of redistribution following guide updates. The master list is always available online, …

6th Annual Faculty Days Presentations Now In The Jdc, Daniel G. Kipnis, Msi

Jefferson Digital Commons News

Check out the innovative teaching methods and ideas your colleagues are using in their courses. Access all 14 presentations from the recent 6th Annual Faculty Days in the Jefferson Digital Commons.

Transcription Factor Cooperativity In Early Adipogenic Hotspots And Super-Enhancers, Rasmus Siersbæk, Atefeh Rabiee, Ronni Nielsen, Simone Sidoli, Sofie Traynor, Anne Loft, Lars La Cour Poulsen, Adelina Rogowska-Wrzesinska, Ole N. Jensen, Susanne Mandrup

School of Pharmacy Faculty Articles

It is becoming increasingly clear that transcription factors operate in complex networks through thousands of genomic binding sites, many of which bind several transcription factors. However, the extent and mechanisms of crosstalk between transcription factors at these hotspots remain unclear. Using a combination of advanced proteomics and genomics approaches, we identify ~12,000 transcription factor hotspots (~400bp) in the early phase of adipogenesis, and we find evidence of both simultaneous and sequential binding of transcription factors at these regions. We demonstrate that hotspots are highly enriched in large super-enhancer regions (several kilobases), which drive the early adipogenic reprogramming of gene expression. …

Molecular Architecture Of Transcription Factor Hotspots In Early Adipogenesis, Rasmus Siersbæk, Songjoon Baek, Atefeh Rabiee, Ronni Nielsen, Sofie Traynor, Nicholas Clark, Albin Sandelin, Ole N. Jensen, Myong Hee Sung, Gordon L. Hager, Susanne Mandrup

School of Pharmacy Faculty Articles

Transcription factors have recently been shown to colocalize in hotspot regions of the genome, which are further clustered into super-enhancers. However, the detailed molecular organization of transcription factors at hotspot regions is poorly defined. Here, we have used digital genomic footprinting to precisely define factor localization at a genome-wide level during the early phase of 3T3-L1 adipocyte differentiation, which allows us to obtain detailed molecular insight into how transcription factors target hotspots. We demonstrate the formation of ATF-C/EBP heterodimers at a composite motif on chromatin, and we suggest that this may be a general mechanism for integrating external signals on …

Tender Loving Care And The Woman With A History Of Recurrent Pregnancy Loss, Emily L. Gruetzmacher

Yale School of Medicine Physician Associate Program Theses

Recurrent pregnancy loss affects one to five percent of couples. This complication is traumatic for women and has psychological implications, including anxiety and depression. Increased monitoring and support – tender loving care – from clinicians has been shown to positively impact pregnancy outcomes during subsequent pregnancies in women with recurrent pregnancy loss. The affect of tender loving care on pregnancy outcomes and psychological distress is not entirely clear, however. We propose a randomized controlled trial to determine if these associations are statistically significant. Specifically, we will recruit women with any cause for early recurrent pregnancy loss and compare early pregnancy …

Trnas As Regulators Of Biological Processes, Medha Raina, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Transfer RNAs (tRNA) are best known for their role as adaptors during translation of the genetic code. Beyond their canonical role during protein biosynthesis, tRNAs also perform additional functions in both prokaryotes and eukaryotes for example in regulating gene expression. Aminoacylated tRNAs have also been implicated as substrates for non-ribosomal peptide bond formation, post-translational protein labeling, modification of phospholipids in the cell membrane, and antibiotic biosyntheses. Most recently tRNA fragments, or tRFs, have also been recognized to play regulatory roles. Here, we examine in more detail some of the new functions emerging for tRNA in a variety of cellular processes …

Male Clients' Behaviours With And Perspectives About Their Last Male Escort Encounter: Comparing Repeat Versus First-Time Hires, Margaret M. Wolff, Christian Grov, Michael D. Smith, Juline Koken, Jeffrey T. Parsons

Publications and Research

Research on men who have sex with men suggests that condomless anal intercourse occurs more frequently in established sexual relationships. While comparable data regarding male-for-male escorting is unavailable, research implies that many clients seek emotional as well as physical connections with the men they hire. In 2012, 495 male clients, recruited via daddysreviews.com completed an online survey about their last hiring experience. Most participants were from the USA (85.7%), the UK and Canada (3.2% each). In total, 75% of encounters involved an escort hired for the first time; 25% were with a previously hired escort ('repeat encounter'). The client's age, …

Statement Before The House Of Representatives Subcommittee On Health Of The Committee On Energy And Commerce, Fred D. Ledley

Natural & Applied Sciences Faculty Publications

Statement of Fred David Ledley, M.D.
Director, Center for Integration of Science and Industry
Professor, Natural and Applied Sciences and Management
Bentley University, Waltham, MA

Before the House of Representatives
Subcommittee on Health of the
Committee on Energy and Commerce

Hearing: 21st Century Cures: Examining the Role of Incentives in Advancing Treatments and Cures for Patients

June 11, 2014

The Potential Roles Of Hepatocyte Growth Factor (Hgf)-Met Pathway Inhibitors In Cancer Treatment, Rahul A. Parikh, Peng Wang, Jan H. Beumer, Edward Chu, Leonard J. Appleman

Markey Cancer Center Faculty Publications

MET is located on chromosome 7q31 and is a proto-oncogene that encodes for hepatocyte growth factor (HGF) receptor, a member of the receptor tyrosine kinase (RTK) family. HGF, also known as scatter factor (SF), is the only known ligand for MET. MET is a master regulator of cell growth and division (mitogenesis), mobility (motogenesis), and differentiation (morphogenesis); it plays an important role in normal development and tissue regeneration. The HGF-MET axis is frequently dysregulated in cancer by MET gene amplification, translocation, and mutation, or by MET or HGF protein overexpression. MET dysregulation is associated with an increased propensity for metastatic …

Community Level Impacts Associated With The Invasion Of English Ivy (Hedera Spp.) In Forest Park: A Look At The Impacts Of Ivy On Community Composition And Soil Moisture, Sara Rose Copp

Dissertations and Theses

Invasive species degrade ecosystems by altering natural processes and decreasing the abundance and diversity of native flora. Communities with major fluctuations in resource supply allow invasive species to exploit limiting resources making the community prone to invasion. In the Pacific Northwest, urban forests characterized with limited light and seasonally limited soil moisture are being dominated by nonnative English ivy (Hedera spp). Three observational studies were conducted in the Southern end of Forest Park within the Balch Creek Subwatershed in Portland, Oregon in order to understand 1) how English ivy changes over three growing seasons, 2) how the native …

Pattern Of Mutation Rates In The Germline Of Drosophila Melanogaster Males From A Large-Scale Mutation Screening Experiment, Jian-Jun Gao, Xue-Rong Pan, Jing Hu, Li Ma, Jian-Min Wu, Ye-Lin Shao, Shi-Meng Ai, Shu-Qun Liu, Sara A Barton, Ronny C Woodruff, Ya-Ping Zhang, Yun-Xin Fu

Faculty, Staff and Student Publications

The sperm or eggs of sexual organisms go through a series of cell divisions from the fertilized egg; mutations can occur at each division. Mutations in the lineage of cells leading to the sperm or eggs are of particular importance because many such mutations may be shared by somatic tissues and also may be inherited, thus having a lasting consequence. For decades, little has been known about the pattern of the mutation rates along the germline development. Recently it was shown from a small portion of data that resulted from a large-scale mutation screening experiment that the rates of recessive …

Evaluating The Efficacy Of Shaping With A Percentile Schedule To Increase The Duration Of Sustained Interaction Following A Bid For Joint Attention In Children With Autism, Therese Gutbrod

USF Tampa Graduate Theses and Dissertations

This study examined the use of shaping with a percentile schedule to increase the duration of the interaction following a bid for joint attention in children with autism. Specifically, the therapist initiated a bid for joint attention and reinforced longer successive approximations in seconds of sustained interaction with the therapist and activity. A percentile schedule ranked the most recent 10 observations and reinforcement was provided if the current observation equaled the sixth ranking. Most-to-least prompting was used if the child failed to meet the calculated criterion. Shaping with a percentile schedule of reinforcement was effective at increasing the duration of …

How Do Management Treatments Affect Invasive Cattail (Typha X Glauca) And Pore Water Nutrient Concentrations?, Yarency Rodriguez

DePaul Discoveries

Invasive species are problematic for wetland managers, but little is known about how common management treatments influence nutrient cycling or plant responses. This study tested three experimental treatments (mowing, herbiciding, and harvesting (i.e., removal of aboveground biomass)) on several response variables: wetland soil porewater nutrient content (NO₃^-, NH₄⁺, PO₄^-), native plant and invasive-Typha density, and light attenuation through the plant canopy. Seventeen days post-treatment, herbiciding resulted in higher porewater phosphate concentrations (55.63 µg-P/L) than harvesting (8.95 µg-P/L). After 24 days, herbicide had higher porewater phosphate concentration (72.03 µg-P/L) than all …

Investigating The Viability Of Two Stenotrophomonas Maltophilia Isolates After Air-Drying, Samantha Lane, Joanna Brooke

DePaul Discoveries

Abstract

Stenotrophomonas maltophilia is a water-borne infectious bacterium that is found in both clinical (hospitals) and non-clinical environments. This human pathogen is commonly recovered from respiratory tract infections. A recent study at a hospital in Taiwan suggested that dry patient charts can serve as a vehicle of transmission of this bacterium⁷. As S. maltophilia is not commonly isolated from dry surfaces, this current study tested the hypothesis that this pathogen can remain viable for some time on a dry surface. This study was designed to determine how long S. maltophilia could remain viable after air-drying by observing …

Disparities Of Sexually Transmitted Infections And Vaccinations Among Young Men Who Have Sex With Men, Aimee Fata

DePaul Discoveries

This study looks at the socio-demographic factors that are associated with vaccination and infection prevalence in a sample of young men who have sex with men (YMSM). Data were collected through a cross sectional questionnaire that surveyed 200 men between the ages of 16 and 24. Results indicated that there was a clustering of sexually transmitted infections (STIs) in the sample, with STIs significantly associated with one another. Also, the demographics of homelessness during the past 12 months and African American showed significant positive associations between STIs and negative associations between vaccinations. Results suggest the need for better access to …