Medicine and Health Sciences Commons^™

A Twin Study Of Early-Childhood Asthma In Puerto Ricans, Supinda Bunyavanich, Judy L. Silberg, Jessica Lasky-Su, Nathan A. Gillespie, Nancy E. Lange, Glorisa Canino, Juan C. Celedon

Human and Molecular Genetics Publications

Background

The relative contributions of genetics and environment to asthma in Hispanics or to asthma in children younger than 3 years are not well understood.

Objective

To examine the relative contributions of genetics and environment to early-childhood asthma by performing a longitudinal twin study of asthma in Puerto Rican children ≤3 years old.

Methods

678 twin infants from the Puerto Rico Neo-Natal Twin Registry were assessed for asthma at age 1 year, with follow-up data obtained for 624 twins at age 3 years. Zygosity was determined by DNA microsatellite profiling. Structural equation modeling was performed for three phenotypes at ages …

Targeted Apoptotic Effects Of Thymoquinone And Tamoxifen On Xiap Mediated Akt Regulation In Breast Cancer, Shashi Rajput, B. N. P. Kumar, Siddik Sarkar, Subhasis Das, Belal Azab, Prasanna K. Santhekadur, Swadesh K. Das, Luni Emdad, Devanand Sarkar, Paul B. Fisher, Mahitosh Mandal

Human and Molecular Genetics Publications

X-linked inhibitor of apoptosis protein (XIAP) is constitutively expressed endogenous inhibitor of apoptosis, exhibit its antiapoptotic effect by inactivating key caspases such as caspase-3, caspase-7 and caspase-9 and also play pivotal role in rendering cancer chemoresistance. Our studies showed the coadministration of TQ and TAM resulting in a substantial increase in breast cancer cell apoptosis and marked inhibition of cell growth both in vitro and in vivo. Anti-angiogenic and anti-invasive potential of TQ and TAM was assessed through in vitro studies. This novel combinatorial regimen leads to regulation of multiple cell signaling targets including inactivation of Akt and XIAP degradation. …

Physical Activity And Sedentary Behavior Of Cancer Survivors And Non-Cancer Individuals: Results From A National Survey, Roy B. Kim, Allison Phillips, Kirsten Herrick, Marieka Helou, Carlin Rafie, Mitchell S. Anscher, Ross B. Mikkelsen, Yi Ning

Family Medicine and Population Health Publications

Increasing physical activity and decreasing sedentary behavior are associated with a higher quality of life and lower mortality rates for cancer survivors, a growing population group. Studies detailing the behavior of cancer survivors are limited. Therefore, we investigated physical activity and sedentary behavior of cancer survivors using data from the National Health and Nutrition Examination Survey (NHANES) 2007–2010. Participants were those who provided physical activity and sedentary behavior data. Those who were pregnant,old, or10,472 non-cancer participants. After adjustment for age, race, gender, education status, body mass index, and smoking status, cancer survivors (n = 10,472) reported significantly longer duration of …

Cam Use Among Overweight And Obese Persons With Radiographic Knee Osteoarthritis, Kate L. Lapane, Shibing Yang, Rachel Jawahar, Timothy Mcalindon, Charles B. Eaton

Family Medicine and Population Health Publications

Background

Obesity is associated with knee pain and is an independent predictor of incident knee osteoarthritis (OA); increased pain with movement often leads patients to adopt sedentary lifestyles to avoid pain. Detailed descriptions of pain management strategies by body mass index (BMI) level among OA patients are lacking. The objectives were to describe complementary and alternative medicine (CAM) and conventional medication use by BMI level and identify correlates of CAM use by BMI level.

Methods

Using Osteoarthritis Initiative baseline data, 2,675 patients with radiographic tibiofemoral OA in at least one knee were identified. Use of CAM therapies and conventional medications …

Association Between Adverse Childhood Experiences And Diagnosis Of Cancer, Monique J. Brown, Leroy R. Thacker, Steven A. Cohen

Family Medicine and Population Health Publications

Objective

Adverse childhood experiences (ACEs) are linked to multiple adverse health outcomes. This study examined the association between ACEs and cancer diagnosis.

Methods

Data from the 2010 Behavioral Risk Factor Surveillance System (BRFSS) survey were used. The BRFSS is the largest ongoing telephone health survey, conducted in all US states, the District of Columbia, Puerto Rico, Guam and the U.S. Virgin Islands, and provides data on a variety of health issues among the non-institutionalized adult population. Principal component analysis (PCA) was used to derive components for ACEs. Multivariable logistic regression models were used to provide adjusted odds ratios (OR) and …

Pre-Pregnancy Bmi And Weight Gain: Where Is The Tipping Point For Preterm Birth?, Saba W. Masho, Diane Bishop, Meaghan Munn

Family Medicine and Population Health Publications

Background

Obesity in pregnant women is a major problem affecting both the mother and her offspring. Literature on the effect of obesity on preterm birth is inconsistent and few studies have investigated the influence of weight gain during pregnancy. This study examined the effect of maternal pre-pregnancy BMI and weight gain during pregnancy on preterm birth.

Methods

Data from the Collaborative Perinatal Project (CPP) on 45,824 pregnant women with singleton, live-born infants with no severe congenital anomalies was analyzed. Primary outcome variables included preterm (< 37 weeks of gestation), categorized into spontaneous preterm with and without premature rupture of membrane (PROM) and indicated preterm. Maternal BMI was categorized into underweight (BMI < 18.50), normal weight (BMI =1 8.50 – 24.99), overweight (BMI = 25.00 – 29.99), and obese (BMI ≥ 30.00). Multinomial regression analysis was conducted and OR and 95% CI were calculated.

Results

The rate of spontaneous preterm birth with PROM among overweight women decreased with increasing …

Fyn Kinase Is Required For Optimal Humoral Responses, Natalia S. Chaimowitz, Yves T. Falanga, John J. Ryan, Daniel H. Conrad

Microbiology and Immunology Publications

Abstract

The generation of antigen-specific antibodies and the development of immunological memory require collaboration between B and T cells. T cell-secreted IL-4 is important for B cell survival, isotype switch to IgG1 and IgE, affinity maturation, and the development of germinal centers (GC). Fyn, a member of the Src family tyrosine kinase, is widely expressed in many cell types, including lymphocytes. This kinase is known to interact with both the B cell and T cell receptor (BCR and TCR, respectively). While Fyn deletion does not impair the development of immature T cells and B cells, TCR signaling is altered in …

Clinical And Experimental Immunomodulation, Lenin Pavón, Hugo Besedosky, Oscar Bottasso, Rogelio Hernández, Marco Velasco, Roger Loria

Microbiology and Immunology Publications

The inflammatory response is modulated by the concentration of soluble mediators and the coordinated action of different types of immune cells. Furthermore, basic and clinical research has demonstrated that the immune response is regulated by several factors such as: the chemical nature and the concentration of antigen; the route of administration; the cell type involved in the antigen presentation to their specific lymphocytes; and the presence of antibodies and/or immune complexes among other mechanisms.

More recently, it has been described that other signaling molecules like neurotransmitters and hormones can also modulate the immune response. Over time, this information has enabled …

Herpesvirus-Associated Acute Urticaria: An Age Matched Case-Control Study, Arianna Mareri, Stuart P. Adler, Giovanni Nigro

Microbiology and Immunology Publications

Background

Acute and recurrent acute urticaria are often associated with multiple factors including infections and recent data suggest a role for herpesviruses.

Objective

To test the null hypothesis, that is, there is no association of herpesvirus infections with urticaria.

Methods

Thirty-seven patients between one month and 15 years of age were age matched to 37 controls who were healthy or had mild acute respiratory infections but without urticaria. Patients and controls were followed for 1 to 6 years. Diagnostic studies included DNA detection by real-time PCR for herpes simplex virus (HSV) types 1 and 2, Epstein-Barr virus (EBV), cytomegalovirus (CMV) …

Ifn-Γ Rα Is A Key Determinant Of Cd8+ T Cell-Mediated Tumor Elimination Or Tumor Escape And Relapse In Fvb Mouse, Maciej Kmieciak, Kyle K. Payne, Xiang-Yang Wang, Masoud H. Manjili

Microbiology and Immunology Publications

During the past decade, the dual function of the immune system in tumor inhibition and tumor progression has become appreciated. We have previously reported that neu-specific T cells can induce rejection of neu positive mouse mammary carcinoma (MMC) and also facilitate tumor relapse by inducing neu antigen loss and epithelial to mesenchymal transition (EMT). Here, we sought to determine the mechanism by which CD8+ T cells either eliminate the tumor, or maintain tumor cells in a dormant state and eventually facilitate tumor relapse. We show that tumor cells that express high levels of IFN-γ Rα are eliminated by CD8+ T …

Hiv Protease Inhibitors Disrupt Lipid Metabolism By Activating Endoplasmic Reticulum Stress And Inhibiting Autophagy Activity In Adipocytes, Beth S. Zha, Xiaoshan Wan, Xiaoxuan Zhang, Weibin Zha, Jun Zhou, Martin Wabitsch, Guangji Wang, Vijay Lyall, Phillip B. Hylemon, Huiping Zhou

Microbiology and Immunology Publications

Background

HIV protease inhibitors (PI) are core components of Highly Active Antiretroviral Therapy (HAART), the most effective treatment for HIV infection currently available. However, HIV PIs have now been linked to lipodystrophy and dyslipidemia, which are major risk factors for cardiovascular disease and metabolic syndrome. Our previous studies have shown that HIV PIs activate endoplasmic reticulum (ER) stress and disrupt lipid metabolism in hepatocytes and macrophages. Yet, little is known on how HIV PIs disrupt lipid metabolism in adipocytes, a major cell type involved in the pathogenesis of metabolic syndrome.

Methodology and Principal Findings

Cultured and primary mouse adipocytes and …

Inhibition Of P-Glycoprotein By Hiv Protease Inhibitors Increases Intracellular Accumulation Of Berberine In Murine And Human Macrophages, Weibin Zha, Guangji Wang, Weiren Xu, Xuyuan Liu, Yun Wang, Beth S. Zha, Jian Shi, Qijin Zhao, Phillip M. Gerk, Elaine Studer, Phillip B. Hylemon, William M. Pandak Jr., Huiping Zhou

Microbiology and Immunology Publications

Background

HIV protease inhibitor (PI)-induced inflammatory response in macrophages is a major risk factor for cardiovascular diseases. We have previously reported that berberine (BBR), a traditional herbal medicine, prevents HIV PI-induced inflammatory response through inhibiting endoplasmic reticulum (ER) stress in macrophages. We also found that HIV PIs significantly increased the intracellular concentrations of BBR in macrophages. However, the underlying mechanisms of HIV PI-induced BBR accumulation are unknown. This study examined the role of P-glycoprotein (P-gp) in HIV PI-mediated accumulation of BBR in macrophages.

Methodology and Principal Findings

Cultured mouse RAW264.7 macrophages, human THP-1-derived macrophages, Wild type MDCK (MDCK/WT) and human …

Comparative Genomics Of Cryptosporidium, Aurélien J. Mazurie, João M. Alves, Luiz S. Ozaki, Shiguo Zhou, David C. Schwartz, Gregory A. Buck

Microbiology and Immunology Publications

Until recently, the apicomplexan parasites, Cryptosporidium hominis andC. parvum, were considered the same species. However, the two parasites, now considered distinct species, exhibit significant differences in host range, infectivity, and pathogenicity, and their sequenced genomes exhibit only 95–97% identity. The availability of the complete genome sequences of these organisms provides the potential to identify the genetic variations that are responsible for the phenotypic differences between the two parasites. We compared the genome organization and structure, gene composition, the metabolic and other pathways, and the local sequence identity between the genes of these two Cryptosporidium species. Our observations show that the …

Cpg And Interleukin-15 Synergize To Enhance Ifn-Γ Production By Activated Cd8+ T Cells, Dustin Cobb, Siqi Guo, Ronald B. Smeltz

Microbiology and Immunology Publications

Interleukin-15 (IL-15) regulates the development and maintenance of memory CD8⁺ T cells. Paradoxically, we previously reported that IL-15 could enhance CD8⁺ T-cell responses to IL-12, a proinflammatory cytokine required for optimal priming of effector CD8⁺ T cells. To expand the physiological relevance of these findings, we tested IL-15 for its ability to enhance T-cell responses to bacterial CpG. Expectedly, CpG enhanced the production of IFN-γ by CD8⁺ T cells polyclonally activated with anti-CD3. However, addition of IL-15 to CpG-stimulated cultures led to a striking increase in IFN-γ production. The effect of CpG and IL-15 was also …

A Measurement System Experiment To Evaluate The Nosing-To-Nosing Method For Measuring Dimensions Of Steps, Lea S. Jensen, Roger C. Jensen, Craig E. Ross

Safety Health & Industrial Hygiene

Non-uniformity of steps within a flight is a major risk factor for falls. Guidelines and requirements for uniformity of step risers and tread depths assume the measurement system provides precise dimensional values. The state-of-the-art measurement system is a relatively new method, known as the nosing-to-nosing method. It involves measuring the distance between the noses of adjacent steps and the angle formed with the horizontal. From these measurements, the effective riser height and tread depth are calculated. This study was undertaken for the purpose of evaluating the measurement system to determine how much of total measurement variability comes from the step …

Stairway Step Dimensions: Replication Of A Measurement System Study, Christopher Hicks, Roger C. Jensen, Joselynn M. Adams

Safety Health & Industrial Hygiene

This paper reports a replication of a prior measurement system study. The earlier study examined the nosing-to-nosing measurement system for measuring steps in a stairway to determine uniformity. In each study, two individuals measured six flights of stairs on two separate occasions. The difference in the first and second study was the different measurers. Step attributes used to define uniformity are riser height and tread depth. The measurers in each study obtained 744 values of riser height and 672 values of tread depth. The ANOVA for each study indicated that less than 4% of the variance in these attributes was …

University Of South Alabama College Of Medicine Annual Report For 2013-2014, College Of Medicine

COM Annual Reports

The Annual Report of the College of Medicine provides an update on recent accomplishments of our faculty, students, residents, fellows, and staff. Areas highlighted in the report include accomplishments in teaching, research, patient care, community service, and scholarly contributions during the 2013-2014 academic year.

An Empirical Evaluation Of The Us Beer Institute’S Self-Regulation Code Governing The Content Of Beer Advertising, Thomas F. Babor, Ziming Xuan, Donna Damon, Jonathan K. Noel

Health & Wellness Department Faculty Publications and Research

No abstract provided.

Role Of Iron In Ethanol Derived Hepatic Stress, Jesse A. Thornton

Theses, Dissertations and Capstones

Chronic alcohol abuse is the third leading cause of preventable death in the United States. Ethanol metabolism causes liver injury through alterations in hepatic metabolic state, redox status, and acetaldehyde adduct formations. Increased iron absorption is associated with chronic ethanol consumption and may play a role in ethanol induced oxidative stress. We tested the hypothesis that normal labile iron in the liver plays a role in ethanol related pathological stress, using C57/Bl6 mice pair-fed Lieber-DeCarli liquid ethanol diets for 11 and 22 weeks. Normal iron group mice received 55mg/kg iron as ferric citrate, whereas the low iron groups received 5mg/kg. …

Toxicological Effects Of Cerium Oxide Nanoparticle Aggregates On Caenorhabditis Elegans, Steven N. Rogers

Theses, Dissertations and Capstones

Assessing the toxicity and unique reactivity of nanoparticles in biological systems has become an relevant and quickly growing area of environmental toxicology research. The broad use of nanoparticles in industrial and commercial commodities results in exposure of these nano-compounds to the environment, the ecosystems, and humans. While previous data has suggested that cerium oxide (CeO₂) nanoparticles are relatively safe to cultured cells much less is known about the potential toxicity of these materials at the organismal level. In this study we employed transgenic Caenorhabditis elegans (C. elegans) strains to assess the toxicity of CeO₂ nanoparticles under “real-world” …

Perceptions Of Nursing Education Administrators Regarding The Relationship Of Admission Criteria To Student Graduation, Joy F. Cline

Theses, Dissertations and Capstones

This research investigated admission criteria of baccalaureate nursing students related to their success in a multi-state sample of peer universities in the United States. The researcher used mixed methods to collect data that were analyzed using descriptive and phenomenological approaches. The sample of the study was chairpersons from peer institutions of nursing. Data were collected based on the lived experiences of the chairpersons. The purpose of the study was to identify admission criteria that the schools in the study were using and to identify the criteria that predicted success as defined by graduation. The premise of the study was that …

Intraoperative Hyperglycemia In Adult Patients Undergoing An Emergency Craniotomy Following A Traumatic Brain Injury As A Predictor Of Postoperative Outcomes And Mortality, Sara Lynn Hoover

Theses, Dissertations and Capstones

Abstract: The purpose of this study was to determine if there was an association between intraoperative blood glucose ≥150 mg/dl in patients undergoing an emergency craniotomy and Intensive Care Unit (ICU) Length of Stay (LOS), hospital LOS and mortality.

Introduction: Traumatic Brain Injuries (TBI)’s are associated with increased morbidity and mortality. The stress response by the body may cause an increase in blood glucose levels which can cause secondary brain injury. When a patient undergoes an emergency craniotomy following a TBI the increased stress on the body can cause an even higher blood glucose. There is no consensus in …

Traumatic Brain Injury: The Relationship Of Psychosocial Variables And Location Of Injury To Post-Injury Depression, Alicia L. Smith

Theses, Dissertations and Capstones

Traumatic Brain Injury (TBI) affects nearly 1.4 million people in the United States annually, and of these, 10% to 77% will experience post-injury depression. Psychosocial variables such as previous substance and alcohol abuse, prior mental illness, low educational attainment, and poverty have been identified as possible risk factors. Additionally, the location of injury appears to play a key role particularly if the injury occurs in the left hemisphere. This study examined archival data from brain-injured patients in an effort to better understand the factors related to post-TBI depression. Past medical records of brain-injured adults (N = 52) were reviewed …

Energy Balance And Its Relationship To Disordered Eating, Injury, And Menstrual Dysfunction In Soccer Athletes, Emily Lynn Miller

Theses, Dissertations and Capstones

Purpose of the Research: The purpose of this study is to examine the causes of negative energy balance in male and female collegiate soccer players who do not exhibit disordered eating. The study also looked at the prevalence of complications associated with disordered eating, injury and menstrual dysfunction to determine if these would be present in the participants in a negative or positive energy balance regardless of disordered eating status. A questionnaire will examine participants’ disordered eating risk, and a food and activity diary will examine energy balance. A third questionnaire regarding injury and menstrual history will determine their prevalence …

Mike & Molly -- An Other World, Maureen Elizabeth Johnson

Theses, Dissertations and Capstones

This thesis explores the impact of the television show Mike & Molly on the modern debate related to fat in America. The thesis uses the work of Michel Foucault as well as disability scholars such as Lennard Davis and feminist scholars such as bell hooks to examine how a comedy show like Mike & Molly can further disenfranchise fat people in society. The thesis shows that fat makes people an Other in society, and television shows and other forms of comedy that mock those who are fat just reinforce that Other status.

Adequate Ventilation: How Is It Defined And Does It Adequately Warn Consumers?, Christopher Blankenship

Theses, Dissertations and Capstones

The purpose of this research was to clarify and define the term “adequate ventilation.” Upon looking at regulations, court cases, and other elements, it is clear that the term is inadequate to inform consumers and does not instruct consumers on how to achieve proper ventilation. To find out if consumers understand this, random persons were asked to define it. The answers were given a numerical value to show the knowledge trend. The outcome showed that most people do not understand how to achieve proper ventilation. Conclusion: Diagrams showing how to set up a room with ventilation and new instructions are …

Antibiotic-Like Actions Of Vitamin D, Franklin D. Shuler Md, Phd, Josh Hendrix, Adam Short, Sammy Hodroge

Orthopaedics

Vitamin D is a secosteroid hormone that has expanding importance for a healthy lifestyle and disease prevention. A multitude of studies have highlighted that vitamin D acts not only in bone and calcium homeostasis but is critically important for human immunity. The discovery that the storage form of vitamin D (25-hydroxyvitamin D3) can be locally converted to the active form (1,25-hydroxyvitamin D3) in immune cells, epithelial cells and numerous other non-renal tissues highlights the importance of maintaining sufficient stores. When responding to a specific external stimulus, like bacterial invasion, intracrine synthesis of active vitamin D has the ability to regulate …

Improving Patient Flow Through The Implementation Of A Results Pending Treatment Area, Heather L. Bryant

Nursing Theses and Capstone Projects

Abstract

The purpose of this research study is to retrospectively evaluate whether the implementation of a Results Pending Treatment Area (RPTA) altered patient flow patterns and therefore reduced patient length of stay (LOS) for ambulatory patients in a Level One Trauma Center Emergency Department. The research is justified because a reduction in the length of stay for patients is shown to decrease overcrowding, ED wait times, loss of revenue, and diversion while improving patient safety and patient satisfaction. The Roy Adaptation Model was the overarching theoretical conceptual framework utilized to support the research, with a more detailed emphasis on Swanson's …

Improving Faculty Perceptions Of And Intent To Use Simulation: An Intervention Project, Charles Tucker

Nursing Theses and Capstone Projects

Human patient simulation is an innovative teaching strategy that can facilitate practice development and preparation for entry into today's healthcare environment for nursing students. Unfortunately, the use of human patient simulation has been limited due to the perceptions of nursing faculty members. This project sought to explore those perceptions using the Theory of Planned Behavior attributes of attitude, subjective norm, and perceived behavioral controls. A two phase project explored the use of an educational workshop intervention to change faculty perceptions and potentially improve intent to use human patient simulation by the nursing faculty. While the educational workshop intervention demonstrated statistically …

Cultural Bias Education Of Nursing Faculty, Frank Castelblanco

Nursing Theses and Capstone Projects

A cultural bias education seminar was presented to nursing faculty at a private university in Western North Carolina in an attempt to increase the level of cultural competence. Leininger's "Culture Care and Universality"; a nursing theory was used as the theoretical framework for the project and Campinha-Bacote's Inventory for Assessing the Process of Cultural Competency Among Healthcare Professionals, Revised (IAPCC-R) was employed to measure the pre- and post-cultural competence levels of the nursing faculty. The faculty voluntarily completed the pre-assessment, the seminar was completed and 17 participants completed the post assessment. The statistical findings illustrated an increase in total cultural …