Medicine and Health Sciences Commons^™

Electrotransfer Of Single-Stranded Or Double-Stranded Dna Induces Complete Regression Of Palpable B16.F10 Mouse Melanomas, Loree Heller, Vesba Todorovic, Maja Cemazar

Bioelectrics Publications

Enhanced tumor delivery of plasmid DNA with electric pulses in vivo has been confirmed in many preclinical models. Intratumor electrotransfer of plasmids encoding therapeutic molecules has reached Phase II clinical trials. In multiple preclinical studies, a reduction in tumor growth, increased survival or complete tumor regression have been observed in control groups in which vector or backbone plasmid DNA electrotransfer was performed. This study explores factors that could produce this antitumor effect. The specific electrotransfer pulse protocol employed significantly potentiated the regression. Tumor regression was observed after delivery of single-stranded or double-stranded DNA with or without CpG motifs in both …

Visiting Lecturer Will Link Public Health Risks To Climate Change, Public Affairs & News Bureau, Old Dominion University

News Items

No abstract provided.

Regulation Of Hyaluronate Lyase (Hyla) Expression By Regr A Transcriptional Repressor In Streptococcus Pyogenes, Alexis A. Kordis

Biological Sciences Theses & Dissertations

Streptococcus pyogenes, group A streptococci (GAS), causes diseases ranging from asymptomatic to life threatening. Some strains of S. pyogenes produce extracellular hyaluronate lyase (HylA), a potential virulence factor. HylA is an enzyme that degrades hyaluronic acid, which is found in the extracellular matrix of human tissues. The breakdown of the host tissue contributes to the spread of infection. The hylA gene is not constitutively expressed in vitro, which implies regulation.

A proposed regulator of hyaluronate lyase expression is RegR, a LacI/GalR like protein. A vector containing disrupted regR was electro-transformed into S. pyogenes M-type 22 strain 10403. Growth characteristics …

Use Of Immersive Visualization For The Control Of Dental Anxiety During Dental Hygiene Treatment, Carmelo Padrino-Barrios

Dental Hygiene Theses & Dissertations

Purpose: The purpose of this study was to determine the effectiveness of immersive visualization (IV) therapy, a technique that provides visual and stereoscopic display through the use of eyewear, in anxious patients during an oral prophylaxis. Methods: A convenience sample of thirty adults was enrolled. A split mouth design was utilized. Subjects were randomly divided into two groups: group A (use of IV for the right side of the mouth), and group B (use of IV for the left side of the mouth). Subjects received a full mouth oral prophylaxis (removal of supra and subgingival calculus and selective polishing), always …

Student Retention In Bsn Programs, Katherine Pittman Hensley

Educational Foundations & Leadership Theses & Dissertations

This study examined, by use of a researcher-developed survey instrument, perceptions between three groups on reasons why students drop out of nursing programs. Also examined are recommendations from the three groups on how to try to avoid nursing student attrition. Specific groups surveyed included native BSN students, RNB students, and a mixed group of nursing faculty. Survey items were divided into two general groups: student-related issues, and institutional issues. Since RNB students (which include a larger number of non-traditional students) and BSN students (which include more of the native university population of traditional students) have many differences, the author attempted …

In Vitro Selection Of Chloroquine Tolerant Plasmodium Falciparum Parasites, Daniel A. Daley

Biological Sciences Theses & Dissertations

Malaria is among the most devastating human diseases, and a majority of lethal cases are caused by the protozoan parasite, Plasmodiumfalciparum. The emergence of multi-drug resistant P.falciparum is a major obstacle to malaria control and is highlighted by the abandonment of chloroquine (CQ) as a first-line treatment of P.falciparum infections worldwide. Chloroquine resistance (CQR) is associated primarily with mutations in the transmembrane digestive vacuole protein, PfCRT. However, CQR P.falciparum parasites harboring the same mutant pfcrt allele vary in their CQ response, suggesting the CQ response is multigenic in nature. No gene outside of pfcrt is completely associated with CQR. Thus, …

Experiences Of Resident Assistants With Potentially Suicidal Students: Identification, Referral, And Expectations, Katherine M. Bender

Counseling & Human Services Theses & Dissertations

Resident Assistants (RAs), living on campus and tasked with advising students while keeping them safe, are in a position to identify and refer students who may be at risk for suicide or other mental health issues. This study examined RA ability to identify students at risk for suicide, RA comfort in working with students at risk for suicide, RA actions taken when working with students who may be at risk for suicide, and RA expectations for shared information about students the RAs have referred for counseling because they may be at risk for suicide. The study found that RAs report …

91st Annual Meeting Of The Virginia Academy Of Science: Proceedings

Virginia Journal of Science

Proceedings of the 91st Annual Meeting of the Virginia Academy of Science, held at Virginia Polytechnic Institute and State University, May 22-24, 2013.

Distribution, Prevalence, And Genetic Analysis Of Panulirus Argus Virus 1 (Pav1) From The Caribbean Sea, Jessica Moss, Donald C. Behringer Jr., Jeffrey D. Shields, Antonio Baeza, Alfonso Aguilar-Perera, Phillippe G. Bush, Clement Dromer, Alejandro Herrera-Moreno, Lester Gittens, Thomas R. Matthews, Michael R. Mccord, Michelle T. Schärer, Lionel Reynal, Nathanial Truelove, Mark J. Butler Iv

Biological Sciences Faculty Publications

The pathogenic virus Panulirus argus virus 1 (PaV1) was first discovered in Caribbean spiny lobsters Panulirus argus from the Florida Keys (USA) in 1999 and has since been reported in Belize, Mexico, and Cuba; its distribution in the wider Caribbean is unknown. We collected tissue samples from adult spiny lobsters from 30 locations in 14 countries bordering the Caribbean Sea and used molecular diagnostics to assay for the presence of PaV1. PaV1 occurred primarily in the northern areas of the Caribbean, where its prevalence was highest. The virus was not found in lobsters from the southeastern Caribbean, and its prevalence …

Section Abstracts: Medical Science

Virginia Journal of Science

Abstracts of the Medical Science Section for the 91st Annual Virginia Journal of Science Meeting, May 2013

Investigating Similarities And Differences As Measured By The Durel And Gsq Between Three Subgroups Attending A Local Aa Meeting To Develop A Profile Of Long Term Attendees, Keesha Masean Kerns

Counseling & Human Services Theses & Dissertations

Alcoholics Anonymous groups are growing in popularity due to their cost effectiveness and history of dependability. Although the program clearly has the numbers to support its popularity, skeptics continually analyze its claims of effectiveness through research. While research pertaining to AA is diverse, very little is presented concerning factors that contribute to retention in the program. The primary purpose for this quantitative study was to investigate the similarities and differences in the various stages of participants of a sample attending a local AA program, identify variables that contribute to retention in the AA sample, and to use Grounded Theory to …

Patterns Of Gene Expression From Human Costal Cartilage In Relation To The Chest Wall Deformity Pectus Carinatum, Janna E. Grubbs

Biological Sciences Theses & Dissertations

Cartilage deformities within the human chest wall, specifically pectus excavatum (PE) and pectus carinatum (PC) are common (1/400-1/1000) and yet, despite their clinical significance, are some of the least studied disorders pertaining to cartilage [1]. The costal cartilage connecting "false ribs" 8-10 to the sternum is often abnormally grown and can lead to formation of a severely sunken "funnel" chest (PE) or push outwards to form a "pigeon" chest (PC). Both conditions can have impact on the diaphragm, heart, lungs, and psychological function. An established ratio of PE and PC in males to females is 4:1, indicating a sex-linked male …

Comparing Velscope Vx And Traditional Oral Exams In Shisha (Hookah) Smokers: A Pilot Study, Amanda E. Kimball

Dental Hygiene Theses & Dissertations

The purpose of this study was to compare oral findings using two exam types, the VELscope Vx® screening device versus a modified oral examination in detecting oral potentially malignant lesions in shisha smokers.

Methods: Thirty-one participants who reported currently smoking shisha and tobacco were recruited. Participants were identified for smoking habits to include two groups, to include those who reported smoking shisha exclusively and those who reported smoking shisha and tobacco. Each group received both exam types; examiners used a standardized protocol. A health history questionnaire was also collected to assess participant's oral cancer risk factors such as age, race, …

Fluorescent Technology Versus Visual And Tactile Examination In The Detection Of Oral Lesion: A Pilot Study, Hadeel Mohammed Ayoub

Dental Hygiene Theses & Dissertations

Purpose: The purpose of the study was to compare the effectiveness of the VELscope® Vx, versus a visual and tactile intraoral examination in detecting oral lesions in an adult, high risk population. Methods: A convenience sample of 30 participants (17 cigarette smokers and 13 dual addiction smokers) was enrolled. For the purpose of this study, dual addition was defined as cigarettes plus hookah usage. Two trained and calibrated dental hygienists conducted all examinations. Visual and tactile intraoral examinations were conducted, followed by VELscope® Vx florescence examinations. All subjects received an inspection of the lips, labial mucosa, buccal mucosa, floor of …

Novel Architecture Of Costal Cartilage And Implications In Chest Wall Deformities, Anthony J. Asmar

Biological Sciences Theses & Dissertations

Costal cartilage is a type of hyaline cartilage that forms rod-like structures that connect the ribs to the sternum. Deformation of costal cartilage is observed in the chest wall deformities, pectus excavatum and pectus carinatum. Pectus excavatum involves a sternal displacement causing a depression of the chest while pectus carinatum causes a protrusion of the chest. As costal cartilage is not a widely studied tissue, this leaves little knowledge into possible factors involved in the pathogenesis of pectus deformities. Costal cartilage in these deformities has been described as being weakened and may implicate proteoglycans which play an important role in …

Changes In The Dynamics Of Postural And Locomotor Control As A Result Of Varying Task Demands, Kathleen Sherry Thomas

Human Movement Sciences & Special Education Theses & Dissertations

The aim of this study was to examine changes in postural and locomotor control under varying task demands. Three experiments were designed to address the impact that fast walking had on standing posture over time, slow walking had on gait dynamics over time, and the extent to which gait speed interacts with the ability to walk randomly.

For experiment I, the aim was to identify the time course in which postural adaptation occurred while walking at faster than preferred speeds. Postural motion was assessed at specific intervals over a 35-min walking trial. Findings revealed that walking at a faster speed …

Effects Of An Interprofessional Simulation Activity To Improve Students' Perceptions Of Other Healthcare Professions, Chase Raymond Poulsen

STEMPS Theses & Dissertations

Healthcare professionals have historically been educated and trained by members of their own profession within a curriculum that reinforces their individual discipline-specific strengths. This differentiation has contributed to students having little interaction with other professionals until after they have entered the workforce and consequently little formal education in collaboration or integration. The purpose of this mixed-method study was to evaluate the impact of an interprofessional (IP) collaborative activity on student's perceptions of the others discipline for the improvement in care of medical patients.

The sample population consisted of students from two programs, nursing (n=40) and respiratory therapy (n=33). Students were …

Protective Behavioral Strategy Subtypes As Moderators Of The Relationship Between Alcohol Consumption And Alcohol-Related Problems, Benjamin A. Kite

Psychology Theses & Dissertations

Protective behavioral strategy (or drinking control strategy) use is widely regarded as an effective tool for reducing negative consequences from consuming alcohol (Martens et al., 2005; Martens et al., 2008). Research has shown that frequent protective behavioral strategy use buffers the relationship between alcohol consumption and alcohol-related problems (Borden et al., 2011), and that gender moderates this effect (Benton et al., 2004); however. The present research was used to expand on previous research showing that protective behavioral strategy use can buffer the relationship between alcohol consumption and alcohol-related problems. Further, the assessment of protective behavioral strategy use across gender was …

Teledentistry: A Systematic Review Of Clinical Outcomes, Utilization And Costs, Susan J. Daniel, Lin Wu, Sajeesh Kumar

Dental Hygiene Faculty Publications

Purpose: The purpose of this systematic review is to identify clinical outcomes, health care utilization and costs associated with teledentistry. Relevant databases were searched for articles on teledentistry published until March 2012, reference lists examined and key journals hand searched. Of a possible 58 articles, 19 studies met the inclusion criteria.

Clinical outcomes were generally improved following a teledentistry intervention and satisfaction with teledentistry was consistently high. The few studies examining health care utilization reported mixed findings, but preliminary evidence suggests cost savings for health care facilities.

There is a consistent trend in the literature supporting the efficacy and effectiveness …

Atomic Force Microscopy Characterization Of Collagen ‘Nanostraws’ In Human Costal Cartilage, Michael W. Stacey, Diganta Dutta, Anthony Asmar, H. Elsayed-Ali, R. Kelly Jr., A. Beskok

Bioelectrics Publications

Costal cartilage, a type of hyaline cartilage that bridges the bony ribs and sternum, is relatively understudied compared to the load bearing cartilages. Deformities of costal cartilage can result in deformation of the chest wall, where the sternum is largely pushed toward or away from the spine, pectus excavatum and pectus carinatum, respectively, with each condition having significant clinical impact. In the absence of extensive literature describing morphological features of costal cartilage, we characterized a sample from the costal margin immunohistologically and through atomic force microscopy. We had previously observed the presence of collagen ‘nanostraws’ running the length of costal …

Prevalence And Correlates Of Being Bullied Among In-School Adolescents In Malawi: Results From The 2009 Global School-Based Health Survey, H.W. Kubwalo, A.S. Muula, S. Siziya, S. Pasupulati, E. Rudatsikira

Community & Environmental Health Faculty Publications

Background

Physical and emotional violence against adolescents is a neglected, but growing problem globally. Violence against adolescents negatively affects the victim in terms of physical health, school attendance and performance and social adjustment. The literature on the prevalence and associated factors of bullying against adolescents is sparse in southern Africa outside South Africa. Such data are even sparser for Malawi. The current study was conducted to estimate the prevalence of self-reported bullying and its personal and social correlates.

Methods

A secondary analysis of the Malawi School-Based Student Health Survey (2009) was done. Descriptive analyses were done to describe the sample …

Influence Of Exposure To Benzo[A]Pyrene On Mice Testicular Germ Cells During Spermatogenesis, Hueiwang Anna Jeng, Silvina M. Bocca

Community & Environmental Health Faculty Publications

The objective of this study was to assess the toxicological effect of exposure to benzo(a)pyrene, B[a]P, on germ cells during spermatogenesis. Mice were exposed to B[a]P at 1, 10, 50, and 100 mg/kg/day for 30 days via oral ingestion. Germ cells, including spermatogonia, spermatocytes, pachytene spermatocytes, and round spermatids, were recovered from testes of mice exposed to B[a]P, while mature spermatozoa were isolated from vas deferens. Reproductive organs were collected and weighed. Apoptotic response of germ cells and mature spermatozoa were qualified using the terminal deoxynucleotidyl transferase mediated deoxy-UTP nick end labeling (TUNEL) assay. B[a]P exposure at

Predictive Effects Of Good Self-Control And Poor Regulation On Alcohol-Related Outcomes: Do Protective Behavioral Strategies Mediate?, Matthew R. Pearson, Benjamin A. Kite, James M. Henson

Psychology Faculty Publications

In the present study, we examined whether use of protective behavioral strategies mediated the relationship between self-control constructs and alcohol-related outcomes. According to the two-mode model of self-control, good self-control (planfulness; measured with Future Time Perspective, Problem Solving, and Self-Reinforcement) and poor regulation (impulsivity; measured with Present Time Perspective, Poor Delay of Gratification, Distractibility) are theorized to be relatively independent constructs rather than opposite ends of a single continuum. The analytic sample consisted of 278 college student drinkers (68% women) who responded to a battery of surveys at a single time point. Using a structural equation model based on the …

Impulsivity Like Traits And Risky Driving Behaviors Among College Students, Matthew R. Pearson, Elaine M. Murphy, Ashley N. Doane

Psychology Faculty Publications

The present study examined the predictive effects of five impulsivity-like traits (Premeditation, Perseverance, Sensation Seeking, Negative Urgency, and Positive Urgency) on driving outcomes (driving errors, driving lapses, driving violations, cell phone driving, traffic citations, and traffic collisions). With a convenience sample of 266 college student drivers, we found that each of the impulsivity-like traits was related to multiple risky driving outcomes. Positive Urgency (tendency to act impulsively when experiencing negative affect) was the most robust predictor of risky driving outcomes. Positive Urgency is a relatively newly conceptualized impulsivity-like trait that was not examined in the driving literature previously, suggesting a …

I'M Still Standing: Five Strategies To Survive Going Back To School, Janice Hawkins

Nursing Faculty Publications

[First paragraph] Making the decision to return to school for a PhD was difficult for me. I feared the unknown but inescapable lifestyle adjustments. I’m resistant to change. When facing life’s cold waters, I wade, rather than plunge. There’s no wading into a PhD program. In spite of my cautious nature, I took the plunge.

Cell Responses Without Receptors And Ligands, Using Nanosecond Pulsed Electric Fields (Nspefs), Stephen J. Beebe

Bioelectrics Publications

No abstract provided.

Bioelectrics In Basic Science And Medicine: Impact Of Electric Fields On Cellular Structures And Functions, Stephen J. Beebe

Bioelectrics Publications

Bioelectrics is a new interdisciplinary field that investigates electric field effects on cell membranes and other cellular components. It incorporates four main technologies, including electroporation, nanosecond pulsed electric fields, picosecond pulsed electric fields and cold plasmas. The parent technology in Bioelectrics is electroporation, which uses milli- and/or micro-second electric pulses to permeabilize cells and tissues, for delivery of membrane impermeable molecules. It is now being used for electro-gene delivery, with vascular endothelial growth factor, for revascularization in wound healing and cardiovascular and peripheral vascular disease. Plasmids expressing IL-12 are being delivered for immune system activation in melanoma treatment, now in …

Facilitation Of Electroporative Drug Uptake And Cell Killing By Electrosensitization, Olga N. Pakhomova, Betsy W. Gregory, Andrei G. Pakhomov

Bioelectrics Publications

Cell permeabilization by electric pulses (EP), or electroporation, is widely used for intracellular delivery of drugs and plasmids, as well as for tumour and tissue ablation. We found that cells pre-treated with 100-mus EP develop delayed hypersensitivity to subsequent EP applications. Sensitizing B16 and CHO cells by splitting a single train of eight 100-mus EP into two trains of four EP each (with 5-min. interval) decreased the LD(50) 1.5-2 times. Sensitization profoundly enhanced the electroporation-assisted uptake of bleomycin, a cell-impermeable cytotoxic agent accepted for killing tumours by electrochemotherapy. EP exposures that were not lethal per se caused cell death in …

Late Developing Mammary Tumors And Hyperplasia Induced By A Low-Oncogenic Variant Of Mouse Mammary Tumor Virus (Mmtv) Express Genes Identical To Those Induced By Canonical Mmtv, Robert D. Bruno

Medical Diagnostics & Translational Sciences Faculty Publications

Background: The canonical milk-transmitted mouse mammary tumor virus (MMTV) of C3H mice (C3H-MMTV) rapidly induces tumors in 90% of infected animals by 8 months of age. Pro-viral insertions of C3H-MMTV into genomic DNA results in the overexpression of common core insertion site (CIS) genes, including Wnt1/10b, Rspo2, and Fgf3. Conversely, infection by either the endogenous Mtv-1 virus (in C3Hf) or the exogenous nodule-inducing virus (NIV) (in Balb/c NIV) induces premalignant mammary lesions and tumors with reduced incidence and longer latency than C3H-MMTV. Here, we asked whether Mtv-1/NIV affected the expression of core CIS genes.

Findings: We confirmed the presence of …

Fine Tuning A Well-Oiled Machine: Influence Of Nk1.1 And Nkg2d On Nkt Cell Development And Function, Sunil K. Joshi, Mark L. Lang

Bioelectrics Publications

Natural killer T cells (NKT) represent a group of CD1d-restricted T-lineage cells that provide a functional interface between innate and adaptive immune responses in infectious disease, cancer, allergy and autoimmunity. There have been remarkable advances in understanding the molecular events that underpin NKT development in the thymus and in the complex array of functions in the periphery. Most functional studies have focused on activation of T cell antigen receptors expressed by NKT cells and their responses to CD1d presentation of glycolipid and related antigens. Receiving less attention has been several molecules that are hallmarks of Natural Killer (NK) cells, but …