Medicine and Health Sciences Commons^™

Characterization Of Mouse Prostate Tumor-Infiltrating Leukocytes, Daniel Alan Holterman

Theses and Dissertations in Biomedical Sciences

Prostate cancer is the most common form of non-cutaneous cancer among men in the United States and the second most common cause of cancer-related death among men with approximately 189,000 new cases diagnosed and 30,200 deaths of the disease in 2002. Prostate cancer can be a treatable disease but once it becomes metastatic there are no acceptable therapies. For this reason, immunotherapy has been attempted but the results have been disappointing. The TRAMP model was used to evaluate specific interactions between an intact murine immune system and a prostate tumor that expressed a naturally processed weak tumor antigen. Vaccination trials …

Automatic Speaker Identification Using Reusable And Retrainable Binary-Pair Partitioned Neural Networks, Ashutosh Mishra

Electrical & Computer Engineering Theses & Dissertations

This thesis presents an extension of the work previously done on speaker identification using Binary Pair Partitioned (BPP) neural networks. In the previous work, a separate network was used for each pair of speakers in the speaker population. Although the basic BPP approach did perform well and had a simple underlying algorithm, it had the obvious disadvantage of requiring an extremely large number of networks for speaker identification with large speaker populations. It also requires training of networks proportional to the square of the number of speakers under consideration, leading to a very large number of networks to be trained …

The Ecological Ramifications Of Disease And Density In The Caribbean Spiny Lobster, Panulirus Argus, Donald C. Behringer Jr.

Biological Sciences Theses & Dissertations

In 1999, I discovered the first virus known to be pathogenic to any species of lobster. HLV-PA is a pathogenic herpes-like virus that infects juvenile Caribbean spiny lobster, Panulirus argus, in the waters off south Florida (USA), and it alters the behavior and ecology of this species in fundamental ways. Gross signs of HLV-PA infection are lethargy, morbidity, cessation of molting, and discolored, “milky” hemolymph that does not clot. HLV-PA infects the hemocytes of host lobsters, specifically the hyalinocytes and semi-granulocytes, but not the granulocytes. When hemolymph from infected donors was injected into healthy juvenile lobsters, 90% of the …

A Qualitative Study To Analyze The Effectiveness Of A Researcher Designed Tool In Documenting Outcomes Of Caring Encounters, Both Individually And Over Time, Karen S. Ackerman

Theses and Graduate Projects

Background: Research into the documentation of quality-of-care outcomes is pivotal to developing nursing theory, increasing the knowledge base of nursing, and highlighting nursing accomplishments. This thesis presents a researcher-designed, agency-specific tool as one way of describing and documenting the outcomes of caring encounters. Purpose: The purpose of this study was to analyze the effectiveness of a researcher-designed tool in documenting the outcomes of caring encounters both individually and over time. Method: A descriptive research design was used to study the implementation of the tool. Watson's Theory of Human Caring served as the synthesizing framework. Results: Outcome tools applied to 34 …

Transmission Of Mycobacterium Tuberculosis Associated With Failed Completion Of Treatment For Latent Tuberculosis Infection - Chickasaw County, Mississippi, June 1999-March 2002, T. Chamblee, D. Hartley, M. Holcombe, K. Parham, P. Upchurch, R. M. Webb, Alyssa G. Robillard, L. Diem, B. Metchock, N. Deluca, P. D. Mcelroy, T. Navin, W. Walton, P. K. Dewan

Faculty Publications

During June 1999-March 2002, a total of 16 tuberculosis (TB) cases were reported from Chickasaw County, Mississippi (2000 population: 19,440), corresponding to annual TB incidences of 20.5-27.6 cases per 100,000 population. In comparison, annual TB incidences for Mississippi during the same period decreased from 7.8 to 5.4 cases per 100,000 population. This report summarizes the results of an investigation of the patients and their contacts and demonstrates the need for strategies to increase the proportion of infection contacts that successfully complete treatment for latent TB infection (LTBI).

Estimating The Accuracy Of Polymerase Chain Reaction-Based Tests Using Endpoint Dilution, Jim Hughes, Patricia Totten

UW Biostatistics Working Paper Series

PCR-based tests for various microorganisms or target DNA sequences are generally acknowledged to be highly "sensitive" yet the concept of sensitivity is ill-defined in the literature on these tests. We propose that sensitivity should be expressed as a function of the number of target DNA molecules in the sample (or specificity when the target number is 0). However, estimating this "sensitivity curve" is problematic since it is difficult to construct samples with a fixed number of targets. Nonetheless, using serially diluted replicate aliquots of a known concentration of the target DNA sequence, we show that it is possible to disentangle …

Gene Therapy For Pediatric Cancer: State Of The Art And Future Perspectives, Ettore Biagi, Catherine Bollard, Raphael Rousseau, Malcolm Brenner

Faculty, Staff and Students Publications

While modern treatments have led to a dramatic improvement in survival for pediatric malignancy, toxicities are high and a significant proportion of patients remain resistant. Gene transfer offers the prospect of highly specific therapies for childhood cancer. "Corrective" genes may be transferred to overcome the genetic abnormalities present in the precancerous cell. Alternatively, genes can be introduced to render the malignant cell sensitive to therapeutic drugs. The tumor can also be attacked by decreasing its blood supply with genes that inhibit vascular growth. Another possible approach is to modify normal tissues with genes that make them more resistant to conventional …

Ada News - 03/17/2003, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Incidental Diagnosis Of Diseases On Un-Enhanced Helical Computed Tomography Performed For Ureteric Colic, Nazim A. Ahmad, M Hammad Ather, Jeffrey Rees

Section of Urology

Background: Patients presenting in the emergency room with flank pain suggestive of acute ureteric colic may have alternative underlying conditions mimicking ureteric stones. An early diagnosis and appropriate treatment for other causes of flank pain is important. The majority of centers around the world are increasingly using un-enhanced helical CT (UHCT) for evaluation of ureteric colic. This study was conducted to determine the incidence and spectrum of significant incidental diagnoses established or suggested on UHCT performed for suspected renal/ureteric colic.Methods: Urologist and radiologist reviewed 233 consecutive UHCT, performed for suspected renal/ureteral colic along with assessment of the medical records. Radiological …

Medical Education Digest, Vol. 5 No. 2 (March 15, 2003), Nova Southeastern University

Medical Education Digest

No abstract provided.

Ohio Academy Of Medical History Annual Meeting Program, March 15, 2003, Ohio Academy Of Medical History

Annual Conference Programs

This tri-fold program is from the annual meeting of the Ohio Academy of Medical History held on March 15, 2003 in Dayton, Ohio. The program details the events and presentations of the meeting.

Trends. A Casualty Of War: Suicide As A Response To An American-Led Invasion Of Iraq, Ibpp Editor

International Bulletin of Political Psychology

This Trends article discusses the political psychology of suicide as protest.

Political Authority And Social Cognitions On The War On Terrorism With Global Reach: Airport Security, Terrorism Contingent On A United States-Led Attack On Iraq, Smallpox Vaccinations, Ibpp Editor

International Bulletin of Political Psychology

This article presents analyses of three common social cognitions embraced by many representatives of political authority concerning aspects of the war on terrorism with global reach.

Role Of Proapoptotic Bax In Propagation Of Chlamydia Muridarum (The Mouse Pneumonitis Strain Of Chlamydia Trachomatis) And The Host Inflammatory Response, Jean-Luc Perfettini, David M. Ojcius, Charles W. Andrews Jr., Stanley J. Korsmeyer, Roger G. Rank, Toni Darville

All Dugoni School of Dentistry Faculty Articles

The BCL-2 family member BAX plays a critical role in regulating apoptosis. Surprisingly, bax-deficient mice display limited phenotypic abnormalities. Here we investigate the effect of BAX on infection by the sexually transmitted pathogen,Chlamydia muridarum (the mouse pneumonitis strain ofChlamydia trachomatis). Bax −/−cells are relatively resistant to Chlamydia-induced apoptosis, and fewer bacteria are recovered after two infection cycles from Bax −/− cells than from wild-type cells. These results suggest that BAX-dependent apoptosis may be used to initiate a new round of infection, most likely by releasingChlamydia-containing apoptotic bodies from infected cells that could be internalized by neighboring uninfected cells. Nonetheless, infected …

High Dietary Level Of Synthetic Vitamin E On Lipid Peroxidation, Membrane Fatty Acid Composition And Cytotoxicity In Breast Cancer Xenograft And In Mouse Host Tissue, Ivan L. Cameron, Jesus Munoz, Christopher J. Barnes, W. Elaine Hardman

Biochemistry and Microbiology

Background

d-α-tocopherol is a naturally occurring form of vitamin E not previously known to have antitumor activity. Synthetic vitamin E (sE) is a commonly used dietary supplement consisting of a mixture of d-α-tocopherol and 7 equimolar stereoisomers. To test for antilipid peroxidation and for antitumor activity of sE supplementation, two groups of nude mice bearing a MDA-MB 231 human breast cancer tumor were fed an AIN-76 diet, one with and one without an additional 2000 IU/kg dry food (equivalent to 900 mg of all-rac-α-tocopherol or sE). This provided an intake of about 200 mg/kg body weight per day. The mice …

Is Community Care A Civil Right? The Unfolding Saga Of The Olmstead Decision, Randy Desonia

National Health Policy Forum

This paper lays the groundwork for understanding the implications of the historic U.S. Supreme Court decision in the case of Olmstead v. L.C., which has far-reaching consequences for the long-term care of people with disabilities. The paper reviews the critical components influencing the case: the Medicaid program’s role in funding community-based long-term care; the Americans with Disabilities Act, which serves as the statutory basis for the decision; and the Court’s legal reasoning. The paper also describes the federal and state responses to the ruling and concludes with a brief discussion of some legal issues that will be debated in the …

Who Will Pay For The Adverse Events Resulting From Smallpox Vaccination? Liability And Compensation Issues, Robin J. Strongin, Eileen Salinsky

National Health Policy Forum

This paper summarizes liability and compensation concerns surrounding the smallpox vaccination program announced by President Bush on December 13, 2002. The paper examines the nature of adverse health events that are likely to occur in connection with the smallpox vaccine, assesses the liability protections that have been established for organizations and individuals participating in the vaccination program, and discusses the compensation mechanisms being considered to address the damages incurred by volunteers who may suffer from adverse vaccine reactions. Specifically, the implications of the Federal Tort Claims Act, workers’ compensation programs, and the creation of a new no-fault compensation fund are …

Characterization Of A Gene Encoding Two Isoforms Of A Mitochondrial Protein Upregulated By Cyclosporin A In Activated T Cells, Laurent Mascarell, Rodolphe Auger, Andres Alcover, David M. Ojcius, Thomas Jungas, Veronique Cadet-Daniel, Jean M. Kanellopoulos, Paolo Truffa-Bacchi

All Dugoni School of Dentistry Faculty Articles

Cyclosporin A (CSA) is an immunosuppressor used in organ transplantation. A recent proteomic analysis has revealed that activation of T cells in the presence of CSA induces the synthesis of hundreds of new proteins. Here we used representational difference analysis to characterize some of the corresponding induced genes. After cDNA bank screening we focused on one of these genes, which we named CSA-conditional, T cell activation-dependent (CSTAD) gene. This gene produces two mRNAs resulting from alternative splicing events. They encode two proteins of 104 and 141 amino acids, CSTADp-S and CSTADp-L, for the short and long forms, respectively. FK506 had …

Two Laborers Die From A Scaffolding Fall Inside A Water Tank, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Falls

Two 20 year-old laborers died after falling approximately 25 feet from suspension scaffolding while preparing the inside of a municipal water tank for painting. Neither laborer was wearing fall personal protection equipment at the time of the fatal incident. The laborers were preparing the inside of a 200,000-gallon water tank for painting when one end of the scaffolding dropped after a support cable slipped. The job foreman arrived at the job site and entered the tank to check on the laborers. He found both laborers on the tank floor. Laborer 1 had respiratory vital signs but Laborer 2 did not. …

Hispanic Worker Dies Due To Trench Cave-In, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Other

On December 3, 2002, a 30-year old Hispanic laborer died when an unsupported 8-foot wall of a trench collapsed on him. Three laborers were working in an unsupported trench when one side caved in, burying one laborer (decedent) and partially burying another Hispanic worker. The third laborer managed to escape the trench unharmed. Two of the laborers were Hispanic, the third was a brother to the owner of the company digging the trench. The coroner pronounced the 30-year old laborer dead at the scene due to asphyxiation.

To prevent similar incidents from occurring, Kentucky Fatality Assessment and Control Evaluation personnel …

Persistence Of Virus-Reactive Serum Immunoglobulin M Antibody In Confirmed West Nile Virus Encephalitis Cases, John T. Roehrig, Denis Nash, Beth Maldin, Anne Labowitz, Denise A. Martin, Robert S. Lanciotti, Grant L. Campbell

Publications and Research

Twenty-nine laboratory-confirmed West Nile virus (WNV) encephalitis patients were bled serially so that WNV-reactive immunoglobulin (Ig) M activity could be determined. Of those patients bled, 7 (60%) of 12 had anti-WNV IgM at approximately 500 days after onset. Clinicians should be cautious when interpreting serologic results from early season WNV IgM-positive patients.

Trends. Geropsychology And Global Security, Ibpp Editor

International Bulletin of Political Psychology

This Trends article discusses how to evaluate whether national leaders, as senior citizens, can be considered the best and brightest, and the possible role for cognitive stimulation.

Human Acyl-Coenzyme A:Cholesterol Acyltransferase Expressed In Chinese Hamster Ovary Cells: Membrane Topology And Active Site Location, Song Lin, Xiaohui Lu, Catherine C.Y. Chang, Ta-Yuan Chang

Dartmouth Scholarship

Acyl-CoA:cholesterol acyltransferase (ACAT) is a membrane-bound enzyme that produces cholesteryl esters intracellularly. Two ACAT genes (ACAT1 and ACAT2) have been identified. The expression of ACAT1 is ubiquitous, whereas that of ACAT2 is tissue restricted. Previous research indicates that ACAT1 may contain seven transmembrane domains (TMDs). To study ACAT2 topology, we inserted two different antigenic tags (hemagglutinin, monoclonal antibody Mab1) at various hydrophilic regions flanking each of its predicted TMDs, and expressed the recombinant proteins in mutant Chinese hamster ovary cells lacking endogenous ACAT. Each tagged ACAT2 was expressed in the endoplasmic reticulum as a single undegraded protein band …

Killing For The State: The Darkest Side Of American Nursing, Dave Holmes, Cary H. Federman

Department of Justice Studies Faculty Scholarship and Creative Works

The aim of this article is to bring to the attention of the international nursing community the discrepancy between a pervasive ‘caring’ nursing discourse and the most unethical nursing practice in the United States. In this article, we present a duality: the conflict in American prisons between nursing ethics and the killing machinery. The US penal system is a setting in which trained healthcare personnel practices the extermination of life. We look upon the sanitization of death work as an application of healthcare professionals’ skills and knowledge and their appropriation by the state to serve its ends. A review of …

Androgen-Induced Recruitment Of Rna Polymerase Ii To A Nuclear Receptor–P160 Coactivator Complex, Maggie C. Louie, Hong Qiong Yang, Ai-Hong Ma, Wei Xu, June X. Zou, Hsing-Jien Kung, Hong-Wu Chen

Collected Faculty Scholarship

The androgen receptor, like other nuclear receptors, activates target genes by binding to hormone-responsive enhancers. Here we demonstrate that androgen induces robust recruitment of androgen receptor, members of the p160 coactivator family, and CREB-binding protein/p300 specifically at the distant enhancer of prostate-specific antigen (PSA) gene. Unexpectedly, we found that RNA polymerase II (Pol II) is directly recruited to the enhancer in a hormone-dependent manner, independent of the proximal promoter, and that the isolated PSA enhancer can mediate efficient androgen induction of transcription. Inhibition of the Pol II carboxyl-terminal domain kinase activity with low concentrations of flavopiridol blocks Pol II transfer …

Ada News - 03/03/2003, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Pregnancy, Drug Testing, And The Fourth Amendment: Legal And Behavioral Implications, Brian H. Bornstein

Department of Psychology: Faculty Publications

In its efforts to protect the health of unborn children, the government is increasingly attempting to regulate pregnant women’s conduct. As with other policies affecting pregnant women’s autonomy, these policies have constitutional overtones. In Ferguson v. City of Charleston (2001), the Supreme Court struck down a South Carolina hospital’s policy of testing pregnant women for cocaine and turning positive results over to law enforcement for prosecution. This article discusses the basis of the decision and its legal and behavioral implications. Although the decision came down on the side of pregnant women’s rights, it left unresolved the issue of whether a …

Impact Of Nicotine Withdrawal On Novelty Reward And Related Behaviors, Joyce Besheer, Rick A. Bevins

Department of Psychology: Faculty Publications

The authors tested the decreased reward function hypothesis of nicotine withdrawal using a novel-object place conditioning task. A conditioned place preference was evident in controls and in rats that had experienced 4 nicotine withdrawal days, but not in rats that had experienced 1–3 withdrawal days. This implies that the rewarding properties of interacting with novel objects were not readily associated with the environment in which they were paired. Follow-up experiments eliminated other explanations based on withdrawal-induced failures to process object or environment information. Also, expression of conditioning was not affected, indicating that withdrawal likely altered acquisition. Further investigation into the …

A Cost-Effectiveness Analysis Of A Community-Based Health Promotion Intervention For Adults With Mobility Impairments: Living Well With A Disability, Craig Ravesloot Ph.D., Tom Seekins Ph.D., Catherine Ipsen M.A., Steve Seninger Ph.D., Colleen Murphy-Southwick Ph.D., Lisa Brennan M.L.S., Rita K. Fjeld M.A., Glen W. White Ph.D., Ann Branstetter Ph.D., Vivian Chapman M.A., Amanda Jay M.A., James A. Bovaird M.A., Janet Marquis Ph.D., University Of Montana Rural Institute

Health and Wellness

This document reports on research conducted by the University of Montana and the University of Kansas for the Office of Disability and Health at the Centers for Disease Control and Prevention. The research project, conducted between August 1,1997 and July 31, 2001, was conducted in four separate but related studies. The research was conducted in eight States. We contracted with nine centers for independent living (CIL) to conduct 34 Living Well with a Disability health promotion workshops and to collect outcome measures. These programs included 246 individuals. In the first study, we evaluated the effectiveness and cost outcomes of the …

An Automated Treatment For Jet Lag Delivered Through The Internet., Daniel Z Lieberman

Psychiatry and Behavioral Sciences Faculty Publications

Seventy percent of persons who suffer from psychiatric illness do not receive treatment. Cost-effective, automated treatment can be delivered through the Internet but can be complicated by the lack of professional supervision. This open study piloted a fully automated, publicly available treatment for jet lag as a means of highlighting some of the issues involved in delivering treatment over the Internet. Twenty study participants rated the severity of their jet lag symptoms and their adherence to a light-exposure schedule calculated to accelerate adaptation to a new time zone. A significant negative correlation was observed between how closely participants followed the …