Medicine and Health Sciences Commons^™

Evidence For The Involvement Of A Tsetse Midgut Lectin-Trypsin Complex In Differentiation Of Bloodstream-Form Trypanosomes, L. U. Abubakar, G. Zimba, C. Wells, F. Mulaa, E. O. Osir

All Peer-Reviewed Publications

We have previously described a bloodmeal-induced molecule (lectin-trypsin complex) from the midgut of the tsetse fly, Glossina longipennis, with both lectin and trypsin activities (Osir et al., 1995). In this paper, we report on the isolation of a similar molecule from the midguts of Glossina fuscipes fuscipes and provide direct evidence for its involvement in the development of African trypanosomes. The molecule (native Mr ∼65,700) has two non-covalently linked subunits, Mr ∼28,800 and Mr ∼35,700. The native molecule was found to be capable of inducing differentiation of bloodstream-form trypanosomes into procyclic (midgut forms) in vitro. In the assays, specific antibodies …

High Seasonal Variation In Entomologic Inoculation Rates In Eritrea, A Semi-Arid Region Of Unstable Malaria In Africa, Josephat Shililu, Tewolde Ghebremeskel, Solomon Mengistu, Helen Fekadu, Mehari Zerom, Charles Mbogo, John Githure, Robert Novak, Eugene Brantly, John C. Beier

All Peer-Reviewed Publications

Entomologic studies were conducted in eight villages to investigate the patterns of malaria transmission in different ecologic zones in Eritrea. Mosquito collections were conducted for 24 months between September 1999 and January 2002. The biting rates of Anopheles arabiensis were highly seasonal, with activity concentrated in the wet season between June and October in the highlands and western lowlands, and between December and March in the coastal region. The biting rates in the western lowlands were twice as high as in the western escarpment and 20 times higher than in the coastal region. Sporozoite rates were not significantly different among …

Febrile Seizures: Factors Affecting Risk Of Recurrence In Pakistani Children Presenting At The Aga Khan University Hospital, Z Habib, S Akram, S Ibrahim, B Hasan

Department of Paediatrics and Child Health

Objectives: To (a) describe the effect of temperature rise on seizure recurrence in the ER (b) investigate the effect of age, gender, family and developmental history, type, duration and multiple seizures, past history and number of seizures and treatment given (either late or early) on seizure recurrence in the ER and (c) explore prognostic indicators for seizure recurrence.
Methods: Data from 352 children [ages 3-84 months; 220 males (62.5%) 132 females (37.5%)] was taken using chart reviews for the years January 1998-August 2000 inclusive, from the Pediatric department of the Aga Khan University Hospital. Descriptive statistics, Chi-square, and Discriminant …

Development And Validation Of A Professional Behavior Assessment., Kristie Koenig, Caryn Johnson, Cathleen K Morano, Joseph P Ducette

Department of Occupational Therapy Faculty Papers

Allied health students must quickly socialize into a professional role as they transition from classroom to clinic. In addition to skill development, students must exhibit a host of professional behaviors that facilitate successful interaction with patients, families, and colleagues. There is a need for a valid, reliable assessment of professional behaviors that contribute to clinical competence. This study reports on the development and validation of a professional behavior assessment for occupational therapy students on a part-time clinical rotation (Level I). The Philadelphia Region Fieldwork Consortium (PRFC) Level I Student Evaluation was developed from an initial survey (n = 75) to …

Spectral Measures And Perceptual Ratings Of Hypokinetic Dysarthria, Christopher Dromey

Faculty Publications

Ten individuals with Parkinson disease (PD) and hypokinetic dysarthria were compared with age-matched neurologically normal (NN) speakers on acoustic measures from speaking and vowel phonation tasks as well as on perceptual ratings of connected speech. Listeners identified differences between the groups when asked to judge the severity of vocal and articulatory impairment during reading. Several conventional acoustic measures failed to differentiate speakers with PD from NN speakers. However, measures of the shape (statistical moments) of the long term average spectrum (LTAS) revealed statistically significant differences between the groups across vowel phonation, reading and monologue tasks. The ease of computation of …

A Vision System Performing Lip Shape Analysis For Speech Pathology Research, Christopher Dromey, D J. Lee, Daniel Bates, Xiaoqian Xu

Faculty Publications

We have built a machine vision system to perform lip shape analysis and recognition and to generate 3.D lip models for speech pathology research. The system employs two color cameras that are synchronized to capture image video pairs simultaneously. A novel color segmentation technique that converts the RGB color components to linear color space has been developed to extract lip shape contours. Lip shape of the viseme, the smallest visibly distinguishable unit of speech, is used to identify its corresponding image frame in the recorded image sequences. It is also used to reconstruct the 3-D lip shape model as a …

Embryo And Gamete Development Upon Exposure To Cca Components: Cro3, Cuo, And As2o5, Nerbana Talaat-Elsebaei Mahmoud

Theses and Dissertations in Biomedical Sciences

Production of functional gametes and healthy embryos is essential for proliferation of all vertebrates, especially humans. Many compounds have toxic effects on developing gametes and embryos among which are chromium trioxide (CrO₃), cupric oxide (CuO) and arsenic pentaoxide (As₂O₅) as a mixture (CCA) or individually. Controversy surrounding the safety of CCA-treated wood centers primarily on the toxicity of its components and the potential for these metals to be released from the wood.

The aim of this research is to test the hypothesis that CCA components have deleterious effects on embryo development, oocyte maturation and …

Use Of Intravenous Anti-D In Patients With Refractory And Relapsed Immune Thrombocytopenic Purpura, R. Sajid, Salman Adil, Z. Fadoo, S. Sabir, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Objective: To determine the response to IV anti-D and its comparison with splenectomy as second line therapy in refractory and relapsed cases of ITP, in the Aga Khan University Hospital, Karachi.
Methods: A total of 23 patients with chronic ITP were treated with either anti-D or splenectomy as second line treatment. The patients were assessed for time to achieve a response to second line treatment, duration ofresponse and adverse events.
Results: There were 12 patients in the anti-D group and 11 in the splenectomy group. The mean platelet count at presentation was 9,000/cumm. The mean age was 8.9 …

Fludarabine Induced Immune Thrombocytopenia In A Patient With Cd5 Positive B Cell Chronic Lymphocytic Leukemia, M. Usman, Salman Adil, R. Sajid, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Fludarabine is a purine nucleoside analogue, which inhibits DNA synthesis by inhibiting DNA polymerase and ribonucleoside reductase.1 It affects both dividing and non- dividing cells.2 Fludarabine possesses proven efficacy in the treatment of a variety of indolent B cell lymphoproliferative disorders including chronic lymphocytic leukemia3, low-grade non-Hodgkin's lymphoma4 and Waldenstrom macroglobulinemia.5 It is also a part of conditioning regimes in non-myeloablative bone marrow transplantation.6 The common side effects include myelosuppression, immunosuppression, and neurologic toxicity.7 The rare side effects are immune mediated hemolytic anemia8 and thrombocytopenia.9 Here we describe a case of a middle-aged lady who was diagnosed as B cell …

Cyclosporin Induced Fatal Rhabdomyolysis In A Young Patient With Acquired Aplastic Anemia: A Case Report, M. Usman, S. N. Adil, G. N. Kakepoto, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Cyclosporin is used as an immunosuppressive agent in the management of a variety of autoimmune disorders1 and in patients of organ transplant to suppress host immunity and prevent rejection.2 It is now well established that acquired aplastic anemia is secondary to immune mediated destruction of stem cells in the bone marrow3 and hence Cyclosporin is used as a first line immunosuppressive drug alone or in combination with anti lymphocyte globulin in the management of aplastic anemia.1 Rhabdomyolysis is a rare side effect of Cyclosporin.4 Here we describe a case of fatal rhabdomyolysis in a young patient who received cyclosporin for …

Chromosomal Abnormalities As A Cause Of Recurrent Abortions: A Hospital Experience, M. Azim, A.H. Khan, Z.L. Khilji, J.A. Pal, Mohammad Khurshid

Department of Pathology and Laboratory Medicine

Objective: To analyze the chromosomal abnormalities in couples with history of recurrent abortions.
Design: Retrospective study.
Setting: Cytogenetic section, Department of Pathology at the Aga Khan University Hospital. Karachi, Pakistan. Patients: Three hundred couples with history of recurrent abortions. Main outcome measure: Structural chromosomal abnormalities in carrier couples.
Results: G-banded chromosomes were analyzed from both partners of 300 couples who had had two or more spontaneous abortions. Sixteen (5.3%) couples showed a chromosomal aberration in one of the partners. Seven translocations, 5 inversions, 2 deletions and 2 sex chromosomal abnormalities were identified. There was an increase in …

Good Life In The Balance: A Cross-National Study Of Dutch And Australian Disability Perspectives On Euthanasia And Physician-Assisted Suicide, Erik A. Leipoldt

Theses: Doctorates and Masters

This is a cross-national qualitative study with the purpose of obtaining perspectives held by people with quadriplegia and leading figures in disability movements in the Netherlands and Australia on the issues of euthanasia and physician-assisted suicide (EPAS). A disability voice is not prominent in public debate on EPAS in Australia or the Netherlands, even though people with disabilities are often thought to be vulnerable in relation to EPAS policies. Disability perspectives are potentially valuable in illuminating issues in relation to euthanasia and physician-assisted suicide, because issues of dependence, independence, and individual autonomy play important roles in relation to both EPAS …

An Ethnographic Investigation Of The Relevance Of Shop Floor Culture To Effective Safety Communication In An Australian Minerals Refinery, David R. Leith

Theses: Doctorates and Masters

Many organisations which aim to achieve excellent workplace safety choose 'culture change' as the means to achieve this. They make use of employee communication media to help re-form the values, beliefs, norms and behaviours which are generally thought to comprise culture. However, culture is a complex and profound phenomenon. Successful communication between two culturally separate groups requires each to achieve an understanding of the other, no less so in workplaces than in societies composed of different cultures.Yet even employers who believe in communicating fully with their workforces find it difficult to convey viewpoints other than their own. Their communication tends …

Power, Knowledge And Childbirth Practices : An Ethnographic Exploration In Bangladesh, Kaosar Afsana

Theses: Doctorates and Masters

The intent of this study is to explore why and in what ways rural, poor women in Bangladesh adhere to indigenous birth practices and resist cosmopolitan obstetric care. To understand the complexities of childbirth, a multidimensional framework encompassing culture, gender, socio-economic, political economy and historical perspectives is used. I used ethnographic methods to have deeper understanding of childbirth practices predominantly from women's voices, but strengthened by multiple other voices and my observational experiences. I gathered information in Apurbabari village, the adjacent Thana Health Complex and the Medical College Hospital using in-depth interviews and participant observation, in particular.

Emergency Vehicle Siren Noise: A Potential For Hearing Loss, Douglas A. Riach

Theses: Doctorates and Masters

The purpose of this thesis was to critically investigate the noise produced by the sirens fitted to South Australian Police general patrol vehicles and calculate if this noise was of an intensity to cause noise induced hearing loss. Previous studies in Western Australia and in South Australia examined noise emissions from emergency vehicle sirens for the purpose of selecting the most suitable siren for emergency vehicles, this being the siren emitting the greatest intensity of sound. This study has researched emergency vehicle sirens from the perspective of employee exposure to siren noise. Noise levels were recorded from inside the police …

Covert Violence In Nursing, Susette Bakker

Theses: Doctorates and Masters

There is no official avenue in nursing for the reporting of incidences of covert violence to staff and so they remain unrecorded and often stressful. This study sought to collect data from currently employed nurses concerning covert violence in their workplace, and to collate the information to obtain a valid assessment of this hidden problem. A qualitative methodology was used to report on the experiences of nurses in relation to covert violence directed at them by their peers, other health professionals, patients and patients' families. The participants were all registered nurses employed by a suburban health service. Each was given …

In Pursuit Of Best Practice : Benchmarking Tools And Processes For The Management Of Hazardous Substances In The Workplace, Barry Chesson

Theses: Doctorates and Masters

Many organisations now strive to achieve excellence in various aspects of occupational health and safety. Benchmarking of the techniques and approaches of other organisations is becoming a popular way of bridging gaps and seeking to achieve high levels of performance. There exist many sources of guidance in the form of external and internal standards, regulations, codes of practice, publications by professional institutions and similar. However, there are clear shortfalls in terms of tools and processes needed to identify areas of opportunity and to overcome barriers to the efficient transfer of ideas and techniques from one enterprise to another. This is …

Occupational Hazards And Radiation Safety In Veterinary Practice Including Zoo Veterinary Practice In Australia, Joseph S. Jeyaretnam

Theses: Doctorates and Masters

This thesis contains reviews and research on the occupational hazards of zoo veterinary practitioners in Australia. Although occupational hazards have long been recognised in the veterinary profession, little information is available on the number and magnitude of injuries to veterinarians in Australia, the United Kingdom or the United States. Apart from anecdotal accounts and some limited data, most of the available information is on occupational zoonoses, generally well recognized by veterinarians. Other occupational hazards to which veterinarians are exposed have received scant attention. The veterinary practitioner in a zoo environment has to treat a range of captive wild species which …

Big Boys Don't Cry : Understanding Barriers To Seeking Support For Mental Health Problems Among Adolescent Males, Amanda Miraudo

Theses: Doctorates and Masters

This study explored the possible barriers encountered by adolescents in developing and achieving positive mental health. Male adolescents in Perth, Western Australia were consulted in an attempt to provide insight into the motivating and inhibiting factors influencing help-seeking as a coping strategy. Barriers to seeking help for mental health problems were investigated through the inductive process of grounded theory (Glaser & Strauss, 1967) to obtain a more detailed understanding of help-seeking than previous studies have provided; The study found that the coping responses most frequently enacted by the adolescent males sampled included avoidance, diversion, and private resolution. Help-seeking was far …

A Phenomenological Study Of Partners Of People With Parkinson's Disease Who Demonstrate Communication Changes, Elsie Janet Doherty

Theses: Doctorates and Masters

Parkinson's disease is a common progressive neurological illness for which there is no known cure. While the cardinal signs are tremor, bradykinesia or slowness of movement, muscle rigidity and postural instability (Jahanshahi & Marsden, 1998, p.3) the patient may face an extended number of years experiencing all, or any combination of, the many other manifestations of the disease process. These manifestations include changes to various aspects of communication including loss of voice volume, diminished body language and loss of facial expression. All of these affect both verbal and non verbal communication. Given the progressive nature of Parkinson's disease the patient …

Is It 'Too Bloody Late'? : Older People's Attitudes To Physical Activity And To The Recommendations In The National Physical Activity Guidelines, Joan Jackson

Theses: Doctorates and Masters

Participation in physical activity confers many health benefits by reducing the risk factor for a number of lifestyle related diseases such as diabetes, colon cancer, and heart disease. Other benefits include improved mental and physical well-being. For older people the benefits are even more important; engagement in physical activity extends to such health benefits as reduction in the risk of falls and related potential injuries. However the most significant benefit to older people is that physical activity enables older people to live independently for longer and with a greater sense of well-being. This study explores, through qualitative research, older people's …

Aims, Separation And Attitudinal Factors In Mediation: An Exploratory Investigation, Darryl Frank Menaglio

Theses: Doctorates and Masters

The general aim of this research was to explore issues regarding mediation in family law about which there appears to be uncertainty. Study I investigated: (a) what stakeholders (practitioners and separated spouses) believe the aims of mediation should be; (b) what they believe are the effects of the separation factors, that is, perceived mutuality (whether the decision to separate was made by one or both spouses), perceived status (whether spouses view themselves as Ieavers, lefts or neither), and the attribution and strength of blaming someone for the decision to separate on attitudes towards engaging in mediation; and (c) stakeholders' views …

Examination Of The Magnitude And Timing Of Hip Flexion Torque In High Degree Of Difficulty Forward Somersault Dives On The 3m Springboard, Brendon Ferrier

Theses: Doctorates and Masters

While there has been much research published on the kinematics and kinetics of forward dives from a springboard, very little has been done on the effect the timing and magnitude of hip flexion torques has on forward dives. The purpose of this study was to investigate the relationship between the timing and magnitude of hip flexion torques prior to the take-off and the vertical velocity at take-off in high demand rotational forward dives. Twenty-six elite divers (14 males and 12 females) competing in the United Kingdom leg of the 2001 FINA Diving Grand Prix performed high demand rotational dives. The …

The Effect Of Eccentric Exercise Velocity On Selected Measures Of Muscle Function And Soreness Of The Human Elbow Flexors In Untrained Males And Females, Dale W. Chapman

Theses: Doctorates and Masters

Eccentric contractions, where a muscle is repeatedly lengthened while generating torque, result in decreased muscle function and muscle soreness. This study was designed to determine whether there was a difference in muscle response of the elbow flexors from untrained subjects (n = 12) between a bout of high intensity eccentric exercise at 30°•s-1 (LVE) compared to the equivalent at 210°•s-1 (HVE). Subjects performed 120 seconds of eccentric exercise of the elbow flexors using a Cybex 6000 Isokinetic Dynamometer. At 30°-s-1, a total of 30 repetitions were required whilst at 210°•s-1, 210 contractions were performed (at a 1:7 work/rest ratio). Both …

Clarifying The Lower Incidence Of Birth Defects In The Midwest Of Western Australia : A Study Using Capture Re-Capture Methodology, Donna Baker

Theses: Doctorates and Masters

In April 2000 the Western Australian Birth Defects Registry held its 20th Anniversary Scientific Symposium to signify the first 20 years of the Birth Defects Registry in Western Australia. At the Symposium, significant trends and occurrences in birth defects within Western Australia over the past 20 years were addressed. One factor highlighted in the Registry's 1980-1999 report was that birth defects were reported less frequently in rural regions compared with metropolitan areas. Data collected from the metropolitan area is considered by the Birth Defects Registry to be the most accurate. Therefore, incidence rates of birth defects in this area arc …

The Effects Of Dietary Creatine Monohydrate Supplementation And Resistance Training In Older Men, Andrew Lavender

Theses: Doctorates and Masters

In order to ascertain the effects of creatine monohydrate (CrH2O) supplementation on muscle mass and strength in older males, seventeen volunteers aged between sixty and eighty years were allocated to a creatine or a placebo group. Both groups took part in a training programme of the knee flexors and extensors of one leg only, consisting of knee flexion in a standing position and knee extension in a seated position. An adjustable, weighted cuff was used to provide resistance and subjects increased their weight reps and sets at their own discretion. The creatine group supplemented their training with CrH20 starting with …

Fall Risk Assessment : A Prospective Investigation Of Nurses' Clinical Judgement And Risk Assessment Tools In Predicting Patient Falls In An Acute Care Setting, Helen Myers

Theses: Doctorates and Masters

Falls are a significant problem in acute care, hospital settings, and can have serious consequences, especially for older patients. Fall prevention has therefore been recognised as an important area for research and intervention. In order to target interventions and use resources effectively, a major strategy of many fall prevention programmes has been the development and/or use of risk assessment tools to identify patients who are at high risk of falling. Although many tools have been developed, few have been rigorously tested, and there is currently no evidence to support the clinical utility of fall risk assessment tools. There is a …