Medicine and Health Sciences Commons^™

Asbestos Induces Nuclear Factor Kappa B (Nf-Kappa B) Dna-Binding Activity And Nf-Kappa B-Dependent Gene Expression In Tracheal Epithelial Cells., Yvonne M. Janssen, Aaron Barchowsky, Melinda Treadwell, Kevin E. Driscoll, B T. Mossman

Dartmouth Scholarship

Nuclear factor kappa B (NF-kappa B) is a transcription factor regulating expression of genes intrinsic to inflammation and cell proliferation--features of asbestos-associated diseases. In studies here, crocidolite asbestos caused protracted and dose-responsive increases in proteins binding to nuclear NF-kappa B-binding DNA elements in hamster tracheal epithelial (HTE) cells. This binding was modulated by cellular glutathione levels. Antibodies recognizing p65 and p50 protein members of the NF-kappa B family revealed these proteins in two of the DNA complexes. Transient transfection assays with a construct containing six NF-kappa B-binding DNA consensus sites linked to a luciferase reporter gene indicated that asbestos induced …

Astrocytes Derived From P53-Deficient Mice Provide A Multistep In Vitro Model For Development Of Malignant Gliomas., Alan M. Yahanda Md, Facs, J M Bruner, L A Donehower, R S Morrison

PCI Publications and Projects

Loss or mutation of p53 is thought to be an early event in the malignant transformation of many human astrocytic tumors. To better understand the role of p53 in their growth and transformation, we developed a model employing cultured neonatal astrocytes derived from mice deficient in one (p53 +/-) or both (p53 -/-) p53 alleles, comparing them with wild-type (p53 +/+) cells. Studies of in vitro and in vivo growth and transformation were performed, and flow cytometry and karyotyping were used to correlate changes in growth with genomic instability. Early-passage (EP) p53 -/- astrocytes achieved higher saturation densities and had …

A 55 Year Old Diabetic Presenting With A Rash And Polyneuropathy, S Hyder, H Rasool, H Naqvi, Naila Kayani

Department of Pathology and Laboratory Medicine

No abstract provided.

Method For Inhibiting Angiogenesis With Aurintricarboxylic Acid, Its Analogues Or Salts, Delwood C. Collins, Antonio Gagliardi, Anjan Bhattacharyya

Obstetrics and Genecology Faculty Patents

Aurintricarboxylic acid has potent anti-angiogenic activity and may be used for new therapeutic approaches for diseases of neovascularization including the treatment of solid tumors, diabetic retinopathy and arthritis, among others.

Collection And Utilization Of Child Abuse Statistics In American Indian Communities, Michelle Chino

School of Public Health Faculty Research

Public health research in American Indian communities involves many complex issues that may both help and hinder the development of an effective research methodology and the collection, analysis, and utilization of data. These issues include: 1) the unique strengths and diversity of Indian cultures; 2) the complicated relationships that exist between federal, state, and tribal agencies; 3) the vast distances between communities and services that exist in rural areas; 4) extremely limited human and financial resources; 5) overlapping and often conflicting legal and jurisdictional authorities; and 6) an array of social issues including poverty, substance abuse, modernization, and assimilation. Defining …

Nsu Summer Instruction Master's Program In Life Span Care And Administration: "The Profession And Public Policy" July 14–22,1995, Nova Southeastern University

Abraham S. Fischler College of Education and School of Criminal Justice College Archive

No abstract provided.

Farm-Tractor-Related Fatalities -- Kentucky, 1994, T. W. Struttmann, Carl Spurlock, Susan H. Pollack, E. Moon-Hampton, Steven R. Browning, Robert Mcknight, Reginald Finger

Kentucky Injury Prevention and Research Center Faculty Publications

Fatalities associated with farm tractors are the most common cause of work-related death in the U.S. agricultural industry (1). To characterize farm-tractor-related fatalities in Kentucky, the Kentucky Fatality Assessment and Control Evaluation (KY FACE) Project studied all fatal farm injuries occurring among persons in that state during 1994, the initial year of operation for FACE in Kentucky. This report summarizes the results of that study.

Mid-Atlantic Ethics Committee Newsletter, Summer 1995

Mid-Atlantic Ethics Committee Newsletter

No abstract provided.

Prospects For The Control Of Bolivian Hemorrhagic Fever, Paul E. Kilgore, Clarence J. Peters, James N. Mills, Pierre E. Rollin, Lori Armstrong, Ali S. Khan, Thomas G. Ksiazek

Department of Pharmacy Practice

No abstract provided.

The Beat Newsletter, College Of Medicine

The Beat Newsletter

This is the July, 1995 edition of the Frederick P. Whiddon College of Medicine's newsletter - The Beat.

Contents include:

• Biomedical library renovation at USA medical center
• New director of the office of technology transfer appointed
• USA researchers study how preconditioning helps in heart disease
• Research holds promise for preventing blindness
• 20th class graduates
• Class of 1995 residency assignments
• Awards
• Summer Programs
• University of south Alabama college of medicine summer program participants
• A forty five year debate
• Congratulations...
• Department highlight: Orthopaedic surgery
• College completes self-study

The Relationship Between Risk For Drug Abuse And Meaning In Life, Paul Turner

Masters Theses & Specialist Projects

Drug abuse continues to be one of the most significant problems in the United States today. Attempts to understand drug abuse have produced numerous multidimensional prevention and treatment models. These models have resulted in the identification of psychological, social and biological risk factors related to drug abuse. In this study, the risk for drug abuse was assessed in 311 college students. A questionnaire was developed to measure risk. The questionnaire assessed risk for abuse predicted by the following factors: academic performance, coping and psychological health, religiosity, family and peer drug use, and the individual's past and current drug use. Based …

Basics Of Research (Part 3): Research Study Design, Edward A. Panacek, Cheryl Thompson

Journal Articles: College of Nursing

No abstract provided.

Characterizing The Population In Clinical Trials: Barriers, Comparability, And Implications For Review, Charles Weijer

Philosophy Publications

The definition of the study population for a clinical trial via the criteria for trial eligibility has implications for the validity of the study and its applicability to clinical practice. Though issues of equity regarding the selection of subjects for research have long been a concern of ethicists, issues regarding the impact of subject selection on a trial's generalizability have only recently attracted ethical scrutiny. After a review of the history of the ethics of subject selection, I focus on three empirical questions regarding the generalizability of clinical trials. (1) What proportion of diseased populations are studied in clinical trials? …

Constitutive Alzheimer's-Type Tau Epitopes In A Neuritogenic Rat Cns Cell Line, Mary P. Lambert, Shasta Sabo, Chi Zhang, S. Ather Enam, William L. Klein

Section of Neurosurgery

Paired helical filaments (PHFs) of Alzheimer's disease (AD) largely comprise hyperphosphorylated forms of the cytoskeletal protein tau. AD-type tau phosphoepitopes, detected by various monoclonal antibodies, are absent from normal adult neurons, but recent studies have shown that their expression may contribute to neuritogenesis and axon differentiation in the developing nervous system. Therefore, we have examined a brain nerve cell line that is spontaneously neuritogenic for possible expression of AD-type tau epitopes. The neuritogenic rat brain cell line B103 was found to constitutively produce two AD-related epitopes of tau, detected by cellular immunofluorescence studies with the PHF-1 and Alz-50 monoclonal antibodies. …

A Call To Action: The Institute Of Medicine Report On Emergency Medical Services For Children., J F. Knapp

Manuscripts, Articles, Book Chapters and Other Papers

No abstract provided.

Are Non-Diabetic Women With Abnormal Glucose Screening Test At Increased Risk Of Pre-Eclampsia, Macrosomia And Caesarian Birth?, Khalid S. Khan, Firasat A. Hashmi, J H. Rizvi

Department of Obstetrics & Gynaecology

To determine, in non-diabetic women, the relationship of abnormal glucose screening test, with the incidence of pre-eclampsia, macrosomia and caesarian delivery, from 1988-92, 5646 consecutive women attending antenatal clinic were screened with a glucose challenge test (GCT) on their first visit (usually at 16-20 weeks); those with risk factors i.e., history of unexplained perinatal loss, macrosomia or family member with diabetes and an initial abnormal screening test were rescreened at 28-32 weeks, In 482 cases the GCT was abnormal (plasma glucose value was >140 mg% 2 hours after 75g glucose challenge). Of these, 292 had one or more abnormal critical …

Immune Response To The Carcinoembryonic Antigen In Patients Treated With An Anti-Idiotype Antibody Vaccine, Kenneth A. Foon, Mala Chakraborty, William J. John, Amanda Sherratt, Heinz Köhler, Malaya Bhattacharya-Chatterjee

Markey Cancer Center Faculty Publications

We have generated an IgG1 murine monoclonal anti-idiotype antibody (Ab2) designated 3H1, which mimics a specific epitope on the carcinoembryonic antigen (CEA). Patients with CEA positive tumors are immunologically "tolerant" to CEA. We used 3H1 as a surrogate for CEA for vaccine therapy of 12 patients with advanced colorectal cancer. Each of the patients received a minimum of four intracutaneous injections of aluminum hydroxide precipitated 3H1 at either 1, 2, or 4 mg dosage per injection. 9 of 12 patients demonstrated anti-anti-idiotypic (Ab3) response to 3H1. All nine patients generated specific anti-CEA antibody demonstrated by reactivity with radiolabeled purified CEA; …

The Rat Tram Flap: A Human Analogue?, Geoffrey G. Hallock Md

Department of Surgery

No abstract provided.

Effect Of A No-Smoking Policy Aboard A U. S. Navy Aircraft Carrier, Suzanne L. Hurtado, Scott A. Shappell

Publications

The purpose of this study was to assess the impact if a no-smoking policy aboard the Atlantic Fleet carrier USS THEODORE ROOSEVELT (CVN-71) on the crew's smoking behavior and exposure to ETS, as well as crew attitudes regarding smoking policy. All crew members aboard ship were asked to participate in a baseline and post-intervention survey.

Department Of Radiology-Annual Report-July 1, 1994 To June 30, 1995, David C. Levin

Department of Radiology Administrative Documents and Reports

Department of Radiology Annual Report, July 1, 1994 to June 30, 1995. Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, United States. 120 pages.

Retiree Dies In Tractor Rollover, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

A 63-year-old retired bus driver was pinned underneath a rear tractor tire after it overturned while he was bush hogging. The 37-year-old tricycle tractor, not equipped with a ROPS or a seatbelt, had a bush hog attached. On the morning of the incident, the operator and his brother were mowing a portion of a pasture. At about 9:30 am, the victim started mowing at the bottom of a steep slope, and then progressed toward the top of the ridge. In order to make turns he had to circle around three dogwood trees at the top of the ridge. His brother …

Target Cell Membrane Sialic Acid Modulates Both Binding And Fusion Activity Of Influenza Virus, Maria C. Pedroso De Lima, João Ramalho-Santos, Diana L. Flasher, Vladimir A. Slepushkin, Shlomo Nir, Nejat Düzgüneş

All Dugoni School of Dentistry Faculty Articles

Influenza virus binds to cell surface sialic acid receptors, and following endocytosis fuses with the endosome membrane at low pH. Whether sialic acid plays a role in the virus-cell membrane fusion step is not known. We investigated the effect of the removal of cell membrane sialic acid on the fusion activity of influenza virus (A/PR/8/34 strain) toward human T lymphocytic leukemia (CEM) cells at low pH. Fusion was monitored by fluorescence dequenching of octadecylrhodamine incorporated in the virus membrane. Removal of sialic acid by neuraminidase resulted in a drastic reduction in both viral binding and fusion. The association of the …

Non-Destructive Method For Radiolabelling Biomolecules By Halogenation, Anwar A. Hussain, Lewis W. Dittert

Pharmaceutical Sciences Faculty Patents

A kit and method of non-destructively radiolabelling a biomolecule substrate by halogenation, including the steps of: a) reacting a secondary amine with a chlorinating agent to produce an N-chloro secondary amine, (b) reacting the N-chloro secondary amine with a salt of a radiolabelled halogen to produce a radiolabelled halogenating agent, (c) reacting a solution of the biomolecule substrate with the radiolabelled halogenating agent, and (d) recovering a radiohalogenated biomolecule from the reaction of step (c). The kit and method allow for more complete iodination with a lessening of side reactions such as decomposition of the biomolecule substrate to be labelled.

Ms211: Marnie, Armin Weinberg

Works on Radiation Effects: 1990-2020

A letter of recommendation for Marnie Rose's application to medical school. Marnie matriculated to UTHouston started a Pediatric Residency but passed away due to a glioblastoma. Her family started http://www.drmarnierose.org/ a foundation that continues today. This letter provides a nice summary of her past interest and work related to Chernobyl. See more at Armin Weinberg, PhD Papers and its finding aid.

86 Year Old Farmer Is Killed After Being Hit By Tree Branch, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Tractors/Logging

An 86-year-old male farmer was killed after being struck by a tree limb. The victim was driving a tractor through a pasture checking on his cattle. As he approached a large tree, the extended front end loader, in full upright position, which was attached to the tractor struck a tree branch. The branch broke off and struck the victim causing massive head injury. He was thrown from the tractor and discovered a few hours later by his grandson. The tractor was equipped with a Roll Over Protective Structure (ROPS) and a non-function seat belt. The tractor did not have a …

Increased Abundance Of The Receptor-Type Protein-Tyrosine Phosphatase Lar Accounts For The Elevated Insulin Receptor Dephosphorylating Activity In Adipose Tissue Of Obese Human Subjects, Falyaz Ahmad, Robert V. Considine, Barry J. Goldstein

Department of Medicine Faculty Papers

Protein-tyrosine phosphatases (PTPases) have an essential role in the regulation of the steady-state phosphorylation of the insulin receptor and other proteins in the insulin signalling pathway. To examine whether increased PTPase activity is associated with adipose tissue insulin resistance in human obesity we measured PTPase enzyme activity towards the insulin receptor in homogenates of subcutaneous adipose tissue from a series of six lean and six nondiabetic, obese (body mass index > 30) subjects. The obese subjects had a mean 1.74-fold increase in PTPase activity (P < 0.0001) with a striking positive correlation by linear regression analysis between PTPase activity and body mass index among all of the samples (R = 0.918; P < 0.0001). The abundance of three candidate insulin receptor PTPases in adipose tissue was also estimated by immunoblot analysis. The most prominent increase was a 2.03-fold rise in the transmembrane PTPase LAR (P < 0.001). Of the three PTPase examined, only immunodepletion of LAR protein from the homogenates with neutralizing antibodies resulted in normalization of the PTPase activity towards the insulin receptor, demonstrating that the increase in LAR was responsible for the enhanced PTPase activity in the adipose tissue from obese subjects. These studies suggest that increased PTPase activity towards the insulin receptor is a pathogenetic factor in the insulin resistance of adipose tissue in human obesity and provide evidence for a potential role of the LAR PTPase in the regulation of insulin signalling in disease states.

A Tef-1-Independent Mechanism For Activation Of The Simian Virus 40 (Sv40) Late Promoter By Mutant Sv40 Large T Antigens., Paul Casaz, Phillip W. Rice, Charles N. Cole, Ulla Hansen

Dartmouth Scholarship

Simian virus 40 (SV40) large tumor antigen (T antigen) stimulates the activity of the SV40 late promoter and a number of cellular and other viral promoters. We have characterized the ability of T antigens with mutations in the DNA-binding domain and within the N-terminal 85 residues to activate the SV40 late promoter. T antigens lacking both nonspecific and sequence-specific DNA-binding activities were able to induce the late promoter. Mutations within the N-terminal 85 residues of T antigen diminished activation by less than twofold. Activation by wild-type and most of the mutant T antigens required intact binding sites for the cellular …

Pathways Through Adolescence: An Overview, Lisa J. Crockett, Ann C. Crouter

Department of Psychology: Faculty Publications

Given the connection between adolescent behavior and future well-being, it is important to understand the choices that adolescents make and how patterns of behavior evolve and are maintained during this period. The premise of this volume is that we can describe the behavior patterns young people develop in adolescence, and piece together the decisions that shape their paths through adolescence and into adulthood. Moreover, we can begin to identify the dynamic processes that affect adolescents7 life choices, the emergence of behavior patterns, and the development of lifestyles. This volume seeks to address three questions. How can developmental pathways in adolescence …

"Preface" To Pathways Through Adolescence: Individual Development In Relation To Social Contexts, Lisa J. Crockett, Ann C. Crouter

Department of Psychology: Faculty Publications

The choices that adolescents make about peer relations, risky behavior, investment in school, and income-producing activities set the stage for subsequent development in later adolescence and into adulthood. Moreover, at the aggregate level, these choices have tremendous implications for the quality of life in such key social contexts as families, schools, neighborhoods, and communities. Thus, the pathways that young people follow through adolescence and beyond affect us all. Adolescents' choices, however, are not made in a vacuum; these social contexts provide opportunities--or obstacles--that influence the probability that adolescents will choose certain pathways and not others. Therefore, understanding these critical choices, …

La Résistance Des Gants Aux Mélanges De Produits Chimiques, Jaime Lara, Daniel Drolet, Howard Ketelson

Rapports de recherche scientifique

No abstract provided.