Medicine and Health Sciences Commons^™

One-Step Hot Formamide Extraction Of Rna From Saccharomyces Cerevisiae, Daniel Shedlovskiy, Natalia Shcherbik, Dimitri G Pestov

Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship

Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole yeast cells through a rapid and easily scalable procedure that does not require mechanical cell lysis, phenol, or enzymes. RNA extracted with formamide-EDTA can be directly loaded on gels for electrophoretic analysis without alcohol precipitation. A simplified protocol for downstream DNase treatment and reverse transcription reaction is also included. The formamide-EDTA extraction of …

Effect Of Hyperchloremia On Acute Kidney Injury In Critically Ill Septic Patients: A Retrospective Cohort Study, Lenar Yessayan, Javier A. Neyra, Fabrizio Canepa-Escaro, George Vasquez-Rios, Michael Heung, Jerry Yee, Acute Kidney Injury In Critical Illness Study Group

Internal Medicine Faculty Publications

Background: Hyperchloremia is common in critically ill septic patients. The impact of hyperchloremia on the incidence of acute kidney injury (AKI) is not well studied. We investigated the association between hyperchloremia and AKI within the first 72 h of intensive care unit (ICU) admission.

Methods: 6490 ICU adult patients admitted with severe sepsis or septic shock were screened for eligibility. Exclusion criteria included: AKI on admission, baseline estimated glomerular filtration rate (eGFR) < 15 ml/min/1.73 m², chronic renal replacement therapy, absent baseline serum creatinine data, and absent serum chloride data on ICU admission.

Results: A total of 1045 patients were available for …

Relationship Between Self-Compassion, Self-Perception, And Stuttering Severity, Colleen Hogan

Graduate Independent Studies - Communication Sciences and Disorders

The present study investigated the possibility of a relationship between self-compassion, self-perception, and stuttering severity in adults who stutter. Four adults who identify as people who stutter were administered the Self-Compassion Scale, the Self-Perception Profile for Adults, the Overall Assessment of the Speaker’s Experience of Stuttering, and the Stuttering Severity Instrument. Results indicated a negative correlation between stuttering severity and self-compassion. A relationship was found between self-perception and stuttering severity and self-compassion in only one participant. It was concluded that practices of self-compassion may be beneficial in speech therapy for adults who stutter, and that both self-perception and self-compassion scales …

Zika Virus Encoding Nonglycosylated Envelope Protein Is Attenuated And Defective In Neuroinvasion, Arun Saravanakumar Annamalai, Aryamav Pattnaik, Bikash R. Sahoo, Ezhumalai Muthukrishnan, Sathish Kumar Natarajan, David Steffen, Hiep Vu, Gustavo A. Delhon, Fernando Osorio, Thomas M. Petro, Shi-Hua Xiang, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

ABSTRACT Zika virus (ZIKV), a mosquito-transmitted flavivirus responsible for sporadic outbreaks of mild and febrile illness in Africa and Asia, reemerged in the last decade causing serious human diseases, including microcephaly, congenital malformations, and Guillain-Barré syndrome. Although genomic and phylogenetic analyses suggest that genetic evolution may have led to the enhanced virulence of ZIKV, experimental evidence supporting the role of specific genetic changes in virulence is currently lacking. One sequence motif, VNDT, containing an N-linked glycosylation site in the envelope (E) protein, is polymorphic; it is absent in many of the African isolates but present in all isolates from the …

Evaluating Spatial Variability In Sediment And Phosphorus Concentration-Discharge Relationships Using Bayesian Inference And Self-Organizing Maps, Kristen L. Underwood, Donna M. Rizzo, Andrew W. Schroth, Mandar M. Dewoolkar

College of Engineering and Mathematical Sciences Faculty Publications

Given the variable biogeochemical, physical, and hydrological processes driving fluvial sediment and nutrient export, the water science and management communities need data-driven methods to identify regions prone to production and transport under variable hydrometeorological conditions. We use Bayesian analysis to segment concentration-discharge linear regression models for total suspended solids (TSS) and particulate and dissolved phosphorus (PP, DP) using 22 years of monitoring data from 18 Lake Champlain watersheds. Bayesian inference was leveraged to estimate segmented regression model parameters and identify threshold position. The identified threshold positions demonstrated a considerable range below and above the median discharge—which has been used previously …

Measuring Heightened Attention To Alcohol In A Naturalistic Setting: A Validation Study, Ramey G. Monem, Mark T. Fillmore

Psychology Faculty Publications

Attentional bias to alcohol-related stimuli is believed to be an important contributor to the development and maintenance of drug abuse. There is a considerable body of research examining attentional bias, much of which has typically utilized image-display tasks as a means to assess the phenomenon. Little, however, is known about the nature of this bias in an individual’s natural environment. The current study sought to implement a novel approach to assessing attentional bias in vivo. Participants wore portable eye-tracking glasses that recorded video from their point of view and measured fixation time to objects they observed. They entered a room …

Physical Aggressiveness And Gray Matter Deficits In Ventromedial Prefrontal Cortex, David S. Chester, Donald R. Lynam, Richard Milich, C. Nathan Dewall

Psychology Faculty Publications

What causes individuals to hurt others? Since the famous case of Phineas Gage, lesions of the ventromedial prefrontal cortex (VMPFC) have been reliably linked to physically aggressive behavior. However, it is unclear whether naturally-occurring deficits in VMPFC, among normal individuals, might have widespread consequences for aggression. Using voxel based morphometry, we regressed gray matter density from the brains of 138 normal female and male adults onto their dispositional levels of physical aggression, verbal aggression, and sex, simultaneously. Physical, but not verbal, aggression was associated with reduced gray matter volume in the VMPFC and to a lesser extent, frontopolar cortex. Participants …

Effects Of Environmental Enrichment On Self-Administration Of The Short-Acting Opioid Remifentanil In Male Rats, Rebecca S. Hofford, Jonathan J. Chow, Joshua S. Beckmann, Michael T. Bardo

Psychology Faculty Publications

Background

Opioid abuse is a major problem around the world. Identifying environmental factors that contribute to opioid abuse and addiction is necessary for decreasing this epidemic. In rodents, environmental enrichment protects against the development of low dose stimulant self-administration, but studies examining the effect of enrichment and isolation (compared to standard housing) on the development of intravenous opioid self-administration have not been conducted. The present study investigated the role of environmental enrichment on self-administration of the short-acting μ-opioid remifentanil.

Methods

Rats were raised in an enriched condition (Enr), standard condition (Std), or isolated condition (Iso) beginning at 21 days of …

A Deafness Mechanism Of Digenic Cx26 (Gjb2) And Cx30 (Gjb6) Mutations: Reduction Of Endocochlear Potential By Impairment Of Heterogeneous Gap Junctional Function In The Cochlear Lateral Wall, Ling Mei, Jin Chen, Liang Zong, Yan Zhu, Chun Liang, Raleigh O. Jones, Hong-Bo Zhao

Otolaryngology--Head & Neck Surgery Faculty Publications

Digenic Connexin26 (Cx26, GJB2) and Cx30 (GJB6) heterozygous mutations are the second most frequent cause of recessive deafness in humans. However, the underlying deafness mechanism remains unclear. In this study, we created different double Cx26 and Cx30 heterozygous (Cx26^+/−/Cx30^+/−) mouse models to investigate the underlying pathological changes and deafness mechanism. We found that double Cx26^+/−/Cx30^+/− heterozygous mice had hearing loss. Endocochlear potential (EP), which is a driving force for hair cells producing auditory receptor current, was reduced. However, unlike Cx26 homozygous knockout (Cx26^−/−) mice, the cochlea in Cx26 …

Negative Outcomes Of Teen Sexual Activity: Is There A Regional Effect?, Hannah Latta, Said Shahtahmasebi, Hatim A. Omar

Pediatrics Faculty Publications

A recent victory with respect to teen sexual behavior is the reduction of the national teen birth rate. In 1991, there were 61.8 births per 1000 females aged 15-19. By 2014, this rate plummeted to 24.2 births per 1000 adolescent females. Averages, however, do not reflect state-level variation. For example, in 2014, Kentucky reported 35.3 births per 1000 adolescent females, while New York reported a teen birth rates of 16.1 births per 1000 adolescent females. This report asks: are these discrepancies due to regional factors, and are these differences statistically significant? To examine rural and urban differences in outcomes of …

Hydroxyurea Differentially Modulates Activator And Repressors Of Γ-Globin Gene In Erythroblasts Of Responsive And Non-Responsive Patients With Sickle Cell Disease In Correlation With Index Of Hydroxyurea Responsiveness, Xingguo Zhu, Tianxiang Hu, Meng Hsuan Ho, Yongchao Wang, Miao Yu, Niren Patel, Wenhu Pi, Jeong-Hyeon Choi, Hongyan Xu, Vadivel Ganapathy, Ferdane Kutlar, Abdullah Kutlar, Dorothy Tuan

Pharmacology and Nutritional Sciences Faculty Publications

Hydroxyurea (HU), the first of two drugs approved by the US Food and Drug Administration for treating patients with sickle cell disease (SCD), produces anti-sickling effect by re-activating fetal γ-globin gene to enhance production of fetal hemoglobin. However, approximately 30% of the patients do not respond to HU therapy. The molecular basis of non-responsiveness to HU is not clearly understood. To address this question, we examined HU-induced changes in the RNA and protein levels of transcription factors NF-Y, GATA-1, -2, BCL11A, TR4, MYB and NF-E4 that assemble the γ-globin promoter complex and regulate transcription of γ-globin gene. In erythroblasts cultured …

Simulation As A Multidisciplinary Team Approach In Health Care Programs In An Urban University Setting, Geraldine Fike, Dawn Blue, Guillermo Escalante, Phoebe (Yeon) S. Kim, Jose A. Munoz

Health Science and Human Ecology Faculty Publications

The poster provided here showcases results from a simulation study that began in the Spring Quarter of 2017 at CSU San Bernardino. The results presented here are based on four simulations conducted on campus in our nursing lab space. We incorporated the participation of 44 students in our study.

Patients are cared for by a nurse and multidisciplinary teams which may include physical therapists, social workers, and public health workers; however, students in health care programs usually will not experience necessary scenarios developing needed skills. Although needed skills are learned within the walls of the university they remain in a …

Higher And Lower Order Factor Analyses Of The Temperament In Middle Childhood Questionnaire., Yuliya Kotelnikova, Thomas M Olino, Daniel N Klein, Sarah V M Mackrell, Elizabeth P Hayden

Brain and Mind Institute Researchers' Publications

The Temperament in Middle Childhood Questionnaire (TMCQ) is a widely used parent-report measure of temperament. However, neither its lower nor higher order structures has been tested via a bottom-up, empirically based approach. We conducted higher and lower order exploratory factor analyses (EFAs) of the TMCQ in a large ( N = 654) sample of 9-year-olds. Item-level EFAs identified 92 items as suitable (i.e., with loadings ≥.40) for constructing lower order factors, only half of which resembled a TMCQ scale posited by the measure's authors. Higher order EFAs of the lower order factors showed that a three-factor structure (Impulsivity/Negative Affectivity, Negative …

Non-Pathological Bilious Vomiting Complicating Therapeutic Hypothermia For Hypoxic Ischaemic Encephalopathy In Neonates: A Retrospective Cohort Study, Julie Hukui, Sarah Jones, Kevin Coughlin, Simon Levin, Jennifer Ruth Foster

Paediatrics Publications

Objective Therapeutic hypothermia (TH) for moderate-to-severe neonatal hypoxic ischaemic encephalopathy (HIE) is generally described as safe. We performed this study to determine the incidence of bilious vomiting or bilious drainage (BVD) attributable to TH in this population. Design A single-centre, retrospective cohort study. Setting Neonatal and paediatric intensive care units (NICU and PICU) of a single tertiary care centre. Patients All newborns with HIE who met criteria for TH between 2009 and 2014. Interventions Cases were matched 1:1 for unit of care (NICU vs PICU), gestational age, gender, and Sarnat score with historic controls who did not receive TH. Groups …

Hockey Fans In Training: A Pilot Pragmatic 1 Randomized Controlled Trial, Robert Petrella, Dawn P. Gill, Guangyong Zou, Ashleigh De Cruz, Brendan Riggin, Cassandra Bartol, Karen Danylchuk, Kate Hunt, Sally Wyke, Cindy M. Gray, Christopher Bunn, Merrick Zwarenstein

Lifestyle Research Team

Introduction Hockey Fans in Training (Hockey FIT) is a gender-sensitized weight loss and healthy lifestyle program. We investigated 1) feasibility of recruiting and retaining overweight and obese men into a pilot pragmatic randomized controlled trial and 2) potential for Hockey FIT to lead to weight loss and improvements in other outcomes at 12 wk and 12 months.

Methods Male fans of two ice hockey teams (35–65 yr; body mass index ≥28 kg·m−2) located in Ontario (Canada) were randomized to intervention (Hockey FIT) or comparator (wait-list control). Hockey FIT includes a 12-wk active phase (weekly, coach-led group meetings including provision of …

Achieving Totally Local Anticoagulation On Blood Contacting Devices, Rana Gbyli, Anna Mercaldi, Harihara Sundaram, Kagya Amoako

Mechanical and Industrial Engineering Faculty Publications

The recent years have witnessed an increased activity in biocompatibility research aimed at limiting biomaterial-induced blood coagulation. From 2008 to 2016, a total of $36,946,764.00 USD was awarded in grants to 213 research proposals and as large as 50.4% ($18,627,854.00) of that award monies were distributed to 101 proposals over the fiscal years of FY14 to FY16 alone. However, the complexity in blood responses to biomaterials, variability in blood function between individuals and animal species, and differences in medical device application and test setting all continue to pose difficulties in making a breakthrough in this field. This review focuses on …

Critical Appraisal Tools And Reporting Guidelines For Evidence-Based Practice, Robin Buccheri, Claire Olivia Sharifi

Gleeson Library Faculty and Staff Research and Scholarship

Background: Nurses engaged in evidence-based practice (EBP) have two important sets of tools: Critical appraisal tools and reporting guidelines. Critical appraisal tools facilitate the appraisal process and guide a consumer of evidence through an objective, analytical, evaluation process. Reporting guidelines, checklists of items that should be included in a publication or report, ensure that the project or guidelines are reported on with clarity, completeness, and transparency. Purpose The primary purpose of this paper is to help nurses understand the difference between critical appraisal tools and reporting guidelines. A secondary purpose is to help nurses locate the appropriate tool for the …

As Technologies For Nucleotide Therapeutics Mature, Products Emerge, Jennifer M. Beierlein, Laura M. Mcnamee, Fred D. Ledley

Natural & Applied Sciences Faculty Publications

The long path from initial research on oligonucleotide therapies to approval of antisense products is not unfamiliar. This lag resembles those encountered with monoclonal antibodies, gene therapies, and many biological targets and is consistent with studies of innovation showing that technology maturation is a critical determinant of product success. We previously described an analytical model for the maturation of biomedical research, demonstrating that the efficiency of targeted and biological development is connected to metrics of technology growth. The present work applies this model to characterize the advance of oligonucleotide therapeutics. We show that recent oligonucleotide product approvals incorporate technologies and …

Comparison Of Apical Centring Ability Between Incisal-Shifted Access And Traditional Lingual Access For Maxillary Anterior Teeth, Yoshio Yahata, Yoshiko Masuda, Takashi Komabayashi

Dental Medicine Faculty Publications

The aim of this study was to compare the apical centring ability of incisal-shifted access (ISA) with that of traditional lingual access (TLA). Fifteen three-dimensional printed resin models were prepared from the computed tomography data for a human maxillary central incisor and divided into ISA (n=7), TLA (n=7), and control (n=1) groups. After access preparation, these models were shaped to the working length using K-files up to #40, followed by step-back procedures. An apical portion of the model was removed at 0.5 mm coronal to the working length. Microscopic images of each cutting surface were taken to measure the preparation …

Clinical Cases In Endodontics; Chapter 1: Introduction, Takashi Komabayashi

Dental Medicine Faculty Publications

Chapter 1 of Clinical Cases in Endodontics, a problem-based text that presents a wide range of real cases in endodontics. Clinical Cases in Endodontics presents actual clinical cases, accompanied by academic commentary, that question and educate the reader about essential topics in endodontic therapy. It begins with sets of cases illustrating the most common diagnoses and the steps involved in preparing a treatment plan. Subsequent chapters continue in this style, presenting exemplary cases as the basis of discussing various treatment options, including nonsurgical root canal treatment, re-treatment, periapical surgery, internal and external resorption, emergencies and trauma, and treating incompletely developed …

High Intensity Intervals And Gait Training For A Patient With Heart Failure And Parkinson Disease In A Skilled Nursing Facility: A Case Report, Kelly Fritz

Case Report Papers

Background and Purpose: Congestive Heart Failure is one of the most expensive conditions to manage medically and close to 20% of patients hospitalized for congestive heart failure are readmitted within 30 days. Fifty to seventy percent of people with Parkinson disease experience one or more falls in a 12- month period. The purpose of this case report is to describe the physical therapy management for a patient with acute congestive heart failure and Parkinson disease. Case description: The patient was an 85 year-old male with acute congestive heart failure and Parkinson disease. He received physical therapy at a skilled nursing …

The Role Of Physical Therapy Interventions For An Elderly Patient Following Surgical Fixation Of A Fracture Of The Femoral Shaft: A Case Report, Ashley Push

Case Report Papers

Background and Purpose: This case report contributes to and builds upon established knowledge about the importance of early weight bearing for patients who sustained a fracture and repair. The purpose of this case report is to document this patient’s response to skilled physical therapy in the inpatient setting, including functional mobility, gait training and therapeutic exercise to maximize the patient's functional ability prior to discharge home. Case Description: This report follows an 88-year old female who underwent open reduction and internal fixation of her right femoral shaft after sustaining a non-traumatic fracture. The patient was seen in the acute care …

Strength And Balance Training For A 29-Year-Old Female Who Sustained A Multifocal Stroke: A Case Report, Britney Simonton

Case Report Papers

Background and Purpose: While balance and strengthening are standard interventions for older patients who have sustained a stroke, there is very limited research on the most effective physical therapy rehabilitation for a young female who recently suffered a stroke. Therefore, the purpose of this case report was to investigate a comprehensive physical therapy program that included balance and strength training for a young female who recently sustained a multifocal stroke. Case description: The patient was a 29-year-old female who sustained a multifocal stroke in her left parietal, left frontal, and bilateral occipital lobes. She presented with right lower extremity weakness …

Outpatient Vestibular Rehabilitation For A Patient Three Months Post Acoustic Neuroma Resection: A Case Report, Joel Harrison

Case Report Papers

An acoustic neuroma, also known as a vestibular schwannoma, is a benign and slow growing primary intracranial tumor that originates from Schwann cells of the vestibular nerve within the inner ear. It is estimated that acoustic neuromas occur in 10-20 individuals per 1,000,000 annually in the United States. The tumor itself is often treated through surgical removal; however, the tumor and subsequent surgery frequently lead to vestibular nerve impairment. The vestibular nerve transmits sensory information to the brain from the peripheral vestibular system of the inner ear. Damage to one of the vestibular nerves may lead to vestibular dysfunction on …

Physical Therapy On The Function Of A Patient With Stage Iii Parkinson’S Disease: A Case Report, Kelly Trancygier

Case Report Papers

Background and Purpose: Parkinson’s disease (PD) is typically diagnosed in individuals over the age of 60. The patient in this case report was diagnosed at age 49 which is considered young-onset PD. PD affects 1.5% of the US population over the age of 65. Although there is no cure and the disease itself is not fatal, its effects can be very debilitating. PD typically occurs in five stages. Case Description: The patient was a 54-year-old female that presented to physical therapy with a diagnosis of PD. Initially, she presented with impaired balance, limited range of motion (ROM), impaired standing posture, …

The Effect Of A Stability And Strengthening Program On The Oswestry Disability Index In A 14-Year-Old Patient With Spondylolisthesis: A Case Report, Hormoz Maragoul

Case Report Papers

Background and Purpose: Spondylolisthesis is a condition that describes the anterior translation of the cephalad vertebral segment of the skeletal spine relative to the caudal segment. This condition comes about by forces of gravity acting through the lordotic curve of the lumbar spine. Repetitive extension movements and weakened stabilizing muscles of the trunk like the abdominals and the gluteals may also play a role in contributing to spondylolisthesis. There is an increase in incidence rate of spondylolisthesis in adolescents who participate in sports involving repetitive extension motions. The purpose of this study is to examine the effectiveness of physical therapy …

Balance & Fall Prevention Rehabilitation Program For A 77-Year-Old Patient Following A Trimalleolar Fracture: A Case Report, Kathryn Judd

Case Report Papers

Background and Purpose: Ankle fractures are one of the most common injuries amongst the elderly, with an incidence rate of approximately 174 cases per 100,000 persons per year. Balance training has been a typical component of a physical therapy (PT) program following ankle fractures. Fall prevention programs have been shown to decrease fall risk by 13% to 40%. There is limited research investigating a combined balance training and fall prevention program after an ankle fracture. Therefore, the purpose of this case report was to describe a PT program combining balance training and fall prevention strategies for an elderly patient after …

Outpatient Physical Therapy Management Of A Total Knee Arthroplasty With Severe Contralateral Knee Osteoarthritis: A Case Report, Michael Ikemura

Case Report Papers

Background and Purpose: Osteoarthritis (OA) of the knee is a common cause of severe pain, disability within the community, and dependence on others. If conservative treatments fail, orthopedic surgeons often perform a total knee arthroplasty (TKA), in which damaged articular parts of the knee are resurfaced with prosthetics. To maximize physical function, the underlying cause of gait speed limitation must be determined and treated. The purpose of this case report was to examine the impairments following a TKA with concurrent severe OA of the contralateral knee, develop an effective plan of care, describe physical therapy (PT) interventions, and report the …

Physical Therapy For Low Back Pain With A Focus On Mckenzie Method For Diagnosis And Treatment: A Case Report, Macey N. Berube

Case Report Papers

Background and Purpose: Low back pain (LBP) is a common diagnosis seen in physical therapy (PT). It is thought to affect 80% of the population over their lifespan, and it keeps them out of work, affects daily activity, and decreases quality of life. PT is a noninvasive form of treatment that may include manual therapy, physical exercise, deep heat modalities, or a combination. The purpose of this case report is to review the McKenzie method combined with a conventional PT program for a patient with a recurring episode of chronic LBP. Case Description: The patient was a 72-year-old male referred …

Utilization Of Task-Oriented Training To Restore Independence In A Patient With Encephalitis In The Intensive Care Unit: A Case Report, Victoria Perez

Case Report Papers

Background and Purpose: Bacterial meningitis is acute inflammation of the meninges which can result in significant morbidity and mortality. There are 15,000 to 25,000 cases of bacterial meningitis in the United States yearly and it is one of the top ten causes of infection-related deaths worldwide. Bacterial Meningitis is commonly complicated by encephalitis. The purpose of the case report is to describe physical therapy (PT) rehabilitation in the intensive care unit (ICU) with the goal of increasing the independence of the patient, diagnosed with encephalitis secondary to bacterial meningitis, to prior level of function through task-oriented training in order to …