Medicine and Health Sciences Commons^™

In Silico Analysis Of C-Met Expression And Its Correlation With Metabolic Network In Head And Neck Cancer, Sibi Raj, Dhruv Kumar

Research Symposium

Background: Head and Neck Squamous Cell Carcinoma (HNSCC) is strongly associated with metabolic dysregulations. c-Met activation is important for high glucose induced acquisition of mesenchymal phenotype, survival under high glucose stress in HNSCC cells. Here, we utilise the In silico approach to analyse the c-Met expression in the head and neck cancer data extracted from The Cancer Genome Atlas (TCGA) database and its strong correlation with genes associated with cancer cell metabolism.

Methods: In the current study, our investigations were performed using different bioinformatics tools and databases, including GEPIA (a webserver which extracts data from the Cancer Genome Atlas (TCGA) …

Improving Internal Medicine Residents’ Self Perceived Confidence, Knowledge And Procedural Skill Performing Pap Smears, Azucena Del Real, Adriana C. Betancourth, Leonardo Pozo, Gabriel J. Lora Ferreira, Andres Suarez, Stephanie Luu, Jose Rivera, Christine E. Loftis, Cesar Gutierrez

Research Symposium

Background: Cervical cancer is the second most common cancer in women worldwide; early detection plays a key role in reducing morbidity and mortality. In Texas’ counties lining the border, cervical cancer death rate is 30% higher than the rest of the state.

Methods: A total of 20 Internal medicine residents from the UTRGV - DHR were randomized to an intervention and control groups. Before the intervention, residents had not received any formal training skills on Pap smear technique during their residency. The educational intervention consisted of an eleven-minute video on Thin prep specimen collection and a single-day hands-on training skills …

Implementation Of A Quality Improvement Project For Medical Reconciliation: Outcomes In A Primary Care Residency Clinic, Maria Pesantez Borja, Denisse Ramirez, Rodolfo Garza, Jorge Montes, Dayana Carreras, Kaitlyn Hall, Joseph Caporusso, Dina Hammad, Sergio Saenz, Chelsea H. Chang, Steven Konstantin, Shaza Shaamdem

Research Symposium

Background: Medication reconciliation is the process of creating the most accurate list of medications a patient is taking and comparing it against the physician’s admission, and discharge orders. It is an effective intervention to prevent drug-related events which are the leading cause of medical errors. Medical reconciliation training promotes patient safety and is imperative for a better transition of care.

Methods: We implemented a quality improvement project to promote awareness of medication reconciliation. We aimed to increase compliance of medication to 80% in 3 months and maintain it through March 2021.

Our interventions consisted in reminders to prompt residents to …

Identification And Characterization Of Anticancer Potential Of A Novel Small Molecule, Mortaparibmild, Hazna Noor Meidinna, Anissa Nofita Sari, Sunil C. Kaul, Renu Wadhwa

Research Symposium

Background: The development of new anticancer drugs and treatment modalities form a priority research field. The tumor suppressor protein p53 is frequently mutated or functionally inactivated in a large variety of cancers. Its inactivation by mortalin, a member of the heat shock 70 protein family, has been shown to contribute to carcinogenesis. The small molecule inhibitors of mortalin-p53 interactions have been shown to reactivate p53 yielding apoptosis/growth arrest in cancer cells. Therefore, abrogators of mortalin-p53 interaction have emerged as possible new therapeutic anticancer reagents.

Methods: We performed chemical library screening based on the imaging of mortalin-p53 interaction, leading to the …

Immunepotent Crp Enhances Cyclophosphamide-Induced Cytotoxicity Through A Caspase Independent But Ros Dependent Mechanism In Triple Negative-Breast Cancer Cells, Ana L. Rivera, A. C. Martínez-Torres, C. Rodríguez-Padilla

Research Symposium

Background: Breast cancer (BC) is one of the leading causes of cancer death worldwide. Cyclophosphamide (CYP) remains a mainstay in cancer therapy mainly in the triple negative breast cancer subtype (TNBC) in spite of harmful adverse effects and cell death-resistances. To face this, combination of chemotherapies and immunotherapies has been proposed. IMMUNEPOTENT CRP (ICRP) is an immunotherapy that has cytotoxic effects in several cancer cells without affecting peripheral blood mononuclear cells (PBMC) and CD3+ cells, beside improving clinical parameters of chemotherapy-treated patients. The aim of this study was to evaluate the mechanism of cytotoxicity induced by ICRP in combination with …

Human Ipsc Derived Cardiomyocyte Model Reveals The Transcriptomic Bases Of Covid-19 Associated Myocardial Injury, Kashish Kumar, Satish Kumar, Erica De Leon, Joanne E. Curran, Sarah Williams-Blangero, John Blangero

Research Symposium

Background: Multi-organ complications have been the hallmark of severe COVID-19; cardiac injuries were reported in 20% to 30% of hospitalized COVID-19 patients, although the disease etiology remains poorly understood. This study leveraged genome-wide RNA-sequence data generated using induced pluripotent stem cell (iPSC) differentiated cardiomyocytes (CMs) and in vitro modeling of SARS-CoV-2 infection in CMs, to understand the molecular mechanisms of COVID-19 myocardial injuries for novel diagnostic and therapeutic development.

Methods: Raw RNA-sequence data sets, GSE165242 and GSE150392 were aligned to human genome assembly GRCh38 and gene expressions were quantified. Differentially expressed (DE) genes between experimental groups were identified using moderated …

Hpv Imprints In Western India: The Overlooked Criteria For Cancer Profiling, Ashi R. Thobias, Jayendra B. Patel, Prabhudas P. Patel

Research Symposium

Background: In India, HPV infection detection for cancer-typing has been largely evaded. Especially, data on prevalence of HPV types other than the highly prevalent HPV 16 and 18 are lacking, particularly from the western region. Thus, present study aimed to evaluate prevalence of HPV strains in three most prevailing cancers in India i.e. cervical, oral and oropharyngeal cancer.

Materials & methods: DNA was isolated from tissue samples of 400 cervical cancer cases, 127 oral cancer cases and 75 oropharyngeal cancer cases and endpoint PCR was performed using degenerative primers MY 09/11, GP 5+/6+ and CP I/II. TS-PCR was conducted to …

How Risk Perceptions And Level Of Trust Of Information Influence Individuals’ Health Services Usage, Wan-Lin Chang

Research Symposium

Background: When facing health-related issues, decision-making is not an easy thing. Some people look for advice from health care professionals, and some people trust their family members' and friends’ experiences more. Previous studies in health communication have suggested differences across various demographic groups in information seeking access and skills, including variables related to the knowledge gap assumption and individuals' risk perceptions.

Methods: Multiple linear regression and logistic regression are used to examine how individuals’ health risk perceptions influence their health service usage by analyzing the Health Information National Trends Survey (HINTS) 5 Cycle 4. HINTS conducts national surveys to monitor …

Gene-By-Environment Expression And Calculation Of The Frailty Index, Eron G. Manuosv, Vincent P. Diego, John Blangero, Michael C. Mahaney, Sarah Williams-Blangero

Research Symposium

Background: Frailty can be described as a phenotype (e.g., sarcopenia, reduced grip strength, decreased VO2 max) or as a ratio of deficits, i.e., a Frailty Index (FI). FI predicts survival, death, cognitive impairment, falls, and hospitalizations. Frailty is influenced by both genes and environment. We calculated the FI as the sum of measured deficits divided by the total number of items assessed in a pedigree-based sample of 1,029 Mexican Americans participants in the San Antonio Family Heart Study. We performed a novel search for genotype-by-environment interactions (GXE) influencing FI. Such interactions lead to heritable differences between individuals in their responses …

Folate Receptor Mediated Targeting Enhances Selective Cytotoxicity Of Ashwagandha Derived Drugs To Cancer Cells, Jia Wang, Yue Yu, Sunil C. Kaul, Renu Wadhwa

Research Symposium

Background: Folate receptors (FRs) have been shown to be overexpressed on the surface of a variety of cancer cells and their expression are limited in normal cells and tissues. Since FR strongly binds to folic acid (FA), FA-functionalized nanocarriers have been proposed as a reliable strategy for delivery of anticancer drugs. We have earlier reported that the alcoholic extract of Ashwagandha leaves (i-Extract) and its major cytotoxic component, Withaferin A (Wi-A), have cancer cell killing activity. In the present study, we synthesized a FR-targeting i-Extract nanocomplex (FRi-ExNC) and a FR-targeting Wi-A nanocomposite (FRWi-ANC), by conjugating FA to polyethylene glycol and …

Fat Distribution And Differential Effects On Metabolic Liver Fat Infiltration In Young Mexicans In Reynosa, Mexico: A Collaborative Study Across The U.S.-Mexico Border, Esperanza Milagros Garcia-Oropesa, Adriana Leticia Perales-Torres, Estrella Martinez-Lopez, Claudia Munguia-Cisneros, Edna Nava-Gonzalez, Monserrat Perez-Navarro, Marisol Rosas-Diaz, Neyla Baltazar, Alvaro Diaz-Badillo, Octelina Castillo-Ruiz, Joselin Hernandez-Ruiz, Srinivas Mummidi, Laura Yanneth Ramirez-Quintanilla, Carlos Ramirez-Pfeiffer, Juan Carlos Lopez-Alvarenga

Research Symposium

Introduction: Metabolic-associated fatty liver disease (MAFLD) is a descriptive term for NAFLD (Non-alcoholic) physiopathology associated with obesity. The age of onset linked to body fat distribution is poorly studied. Therefore, we aimed to assess the body fat effect on liver fat infiltration and stiffness (LSt) mediated by insulin resistance (IR).

Methods: After obtaining informed consent, five hundred freshmen from two universities in Reynosa, Mexico (UMAN & UAT) were enrolled in the study. They completed a questionnaire focused on familial cardiometabolic risk and provided anthropometric measurements. In a subset of N=200, we obtained blood samples for biochemical measurements, body fat percentage …

Fornix Volumetric Increase During Aging Associates To Microglia Activation Leading To Defective Cognitive Performance, Marcela Cárdenas Tueme, Luis Ángel Trujillo-Villarreal, Victor Ramírez-Amaya, Eduardo Garza-Villarreal, Alberto Camacho-Morales, Diana Reséndez-Pérez

Research Symposium

Background: Ageing displays a low-grade pro-inflammatory profile in blood and brain. It has been documented proinflammatory cytokines accumulation leading to neuroinflammation during aging. Aged brains integrate pro inflammatory cytokines accumulation, active microglia and volumetric changes which correlates with defective cognitive performance and neurodegeneration.

Methods: Mice from 2-,12- and 20-months-old of age were submitted to different memory tests: Y-maze, Barnes maze, object location test and object location test. Afterwards, we performed structural MRI to evaluate macrostructural changes related to memory and learning regions. Following this, we also evaluated in peripheral blood and in brain tissue the presence of pro-inflammatory cytokines …

Fecal Microbiota Transplant In A Patient With Multi-Drug Resistant Clostridium Difficile (Mdr C.Diff), Baron S. Ekeledo, Semenawit Burka, Maria Najam, Fatimah Bello, Nicole Grigg-Gutierrez

Research Symposium

Introduction: Studies have shown MDR C. diff is the most common bacterial cause of diarrhea in resource rich settings. One of the most feared outcome of MDR C. diff infection is pseudomembranous colitis. Multiple studies indicate FMT is the most effective yet underutilized in treating recurrent C. diff.

Case report: A 73 year old man presented with complaints of diarrhea and fever of 3 days duration. The diarrhea was watery, voluminous, and up to 10 times per day with associated abdominal discomfort. Patient was hospitalized twice in the last 2 months for similar complaints which were treated with oral …

Exploring The Role Of 4-Thiazolidinone Derivatives As Potential Cox-2 Inhibitors And Free Radical Scavenging Agents, Vikram Jeet Singh, Pooja Chawla

Research Symposium

Background: Cyclooxygenase-2 (COX-2) and free radicals has become an important target in the management of various pathological conditions including cancer and inflammation. The pyridine or thiazolidinone has become an emerging scaffold in the drug design and development of COX-2 inhibitors and free radical scavenging agents. 4-Thiazolidinone clubbed pyridine may potentiate each other activity and can emerge as a promising scaffold in the treatment of inflammation/cancer.

Methods: The present work reports synthesis and screening of 4-thiazolidinone-pyridine hybrids with substituted arylidene containing electron-donating group at 5^th position. The compounds were screened for their in-vivo anti-inflammatory activity using carrageenan-induced rat …

Expanding Health Professional Education In The Rio Grande Valley During The Covid-19 Pandemic, Sabrina R. Orta, Samantha G. Alvarado, Shuchita Jhaveri

Research Symposium

Purpose: The COVID-19 Pandemic has prompted innovation in health professional education, such that learners are able to recognize and mitigate healthcare disparities in the outcomes of vulnerable populations. The objective of our project was to increase education on preventing, preparing for, and responding to COVID-19 and other locally prevalent infectious diseases that disproportionately affect RGV communities.

Description: This project had 3 goals: (1) provide learners with virtual patient-interaction simulations (2) provide interactive training modules on the identification, prevention, and treatment of infectious diseases affecting South TX and strategies to increase child vaccinations, and (3) provide learners an opportunity to coordinate …

Engineering Of Functionalized Carbon Nano-Onions Embedded Bsa Nanocomposite Fibers For Stimuli-Responsive Drug Release, Ramiro Manuel Velasco Delgadillo, Narsimha Mamidi

Research Symposium

Background: Advanced drug delivery systems (DDSs) have received enormous attention in biomedical applications due to their pharmacodynamic and pharmacokinetic drug properties. For the present study, poly 4-hydroxyphenyl methacrylate (PHPMA)/CNOs (f-CNOs) inserted bovine serum albumin (BSA) nanofibers were prepared for stimuli-responsive release of Doxorubicin (DOX). Temperature and pH would be altered to study the release of DOX in acidic microenvironments.

Methods: PHPMA were coupled with COOH-CNOs via ester coupling via the sonochemical method to produce PHPMA-CNOs (f-CNOs). Then, f-CNOs/DOX embedded BSA nanofibers were prepared at room temperature using Forcespinning. UV spectra of DOX-loaded nanofibers were studied to investigate the …

Dosimetric Study For Mastectomy Carcinoma Left Breast Cases With Volumetric Modulated Arc Therapy (Vmat), Jyoti Bisht, Meenu Gupta, Ravi Kant, Vipul Nautiyal, Viney Kumar, Rishabh Dobhal, Mushtaq Ahmad, Sunil Saini

Research Symposium

Background: Radiation therapy for breast cancer has evolved from conventional to 3-dimentional radiation therapy (3-DCRT) and through more precise IMRT and VMAT. 3-DCRT is preferred for ca breast treatment as it reduces low dose area in contra lateral lung but with the advances in radiotherapy image guided techniques can deliver precise and lower doses to OARs with better coverage. This study is aimed to evaluate the doses of PTV, heart and ipsi-lateral lung with contra lateral lung doses delivered by VMAT technique.

Method and material: Total 10 patients of carcinoma left breast with mastectomy were selected for VMAT planning with …

Diverse Tregs Population And Effects Of Their Inhibition On Growth Of Oral Cancer Cells, Sadhna Aggarwal, Satya N. Das, Suresh C. Sharma

Research Symposium

Oral squamous cell carcinoma (OSCC) is one of the major cancers affecting in Asian countries. The main causative factor has been tobacco habit. It has been reported that immune dysfunction in these patients is one of the major factors for tumor growth and dissemination that affects disease free survival of the patients.

We assessed the phenotypic and functional characteristics of Regulatory T (Treg) CD4+CD25+FoxP3+subsets in patients with OSCC by multicoloured flow cytometry. Subsequently we investigated the effects their inhibition via TDG on growth of OSCC cell lines in vitro.

An increased (p

Hence, it seems reasonable to assume that modulation …

Development Of A Novel Nir Fluorescent Probe For Breast Cancer Imaging, Neeraj Chauhan, Pallabita Chowdhury, Prashanth Kumar Bhusetty Nagesh, Elham Hatami, Nycol M. Cotto, Benilde Adriano, Meena Jaggi, Subhash C. Chauhan, Murali M. Yallapu

Research Symposium

Background: Early-stage detection is crucial for successful breast cancer treatment and can significantly reduce breast cancer associated death rates. There are several diagnostic approaches available for early breast cancer diagnosis but lack tumor specificity and expose patients with radiation. Therefore, there is a crucial need to develop newer and safer imaging modalities. Indocyanine green (ICG), an FDA approved Near InfraRed (NIR) fluorescent probe-based imaging for early cancer detection and image guided surgery, has gained noticeable attention for the clinical applications as it has high sensitivity, low cost, and real-time visualization/imaging capabilities without ionizing radiation. However, ICG has several limitations associated …

Development And Validation Of A Simple Clinical Construct For Prediction Of New Type 2 Diabetes Mellitus, Xavier Rios, Blanca I. Restrepo, Juan Carlos Lopez-Alvarenga

Research Symposium

Background: Early diagnosis of type 2 diabetes (T2D) can delay its vascular complications, but the active search for T2D results in inefficient use of resources. Simple methods like anthropometry or clinical history could help prioritize laboratory testing in individuals with risk of T2D. We evaluated non-invasive clinical measurements to pre-screen for new T2D patients.

Methods: We used the 2017-2018 NHANES database as the discovery cohort, and identified age, sex, BMI, waist circumference, and family history of T2D (FHD) as predictor variables. Risk of new T2D was analyzed using odds ratios with logistic regression. The diagnostic accuracy was calculated using ROC …

Epoxyazadiradione Exhibit Anti‑Cancer Activities By Modulating Lncrnas Expression In Pancreatic Cancer, Vipin Rai, Nikee Awasthee, Sumit Singh Verma, Subash Chandra Gupta

Research Symposium

Background: Azadirachta indica (neem), a medicinal plant under Meliaceae family, is found in the Indian subcontinent. One of the limonoids, epoxyazadiradione (EPA), is a phytochemical isolated from the seeds of this tree. This is widely used in traditional medicine to treat a variety of human ailments. Although EPA has shown promise against some cancer types, its efficacy against pancreatic cancer and the underlying mechanism remains elusive.

Aim: We examined the anti‑cancer activity of EPA against pancreatic cancer cells. We also examined the underlying mechanism.

Methods: Pancreatic cancer cell lines (PANC-1 and MiaPaCa-2) were used during the study. We performed MTT …

Epidemiological Algorithm And Early Molecular Testing To Prevent Covid-19 Outbreaks In A Mexican Oncologic Center, Moises González-Escamilla, Diana C. Peréz-Ibave, Carlos H. Burciaga-Flores, Vanessa N. Ortiz-Murillo, Genaro A. Ramírez-Correa, Patricia Rodríguez-Niño, Rafael Piñeiro-Retif, Hazyadee F. Rodríguez-Gutiérrez, Orlando D. Solis-Coronado, Juan F. González-Guerrero, Oscar Vidal-Gutiérrez, María Lourdes Garza-Rodríguez

Research Symposium

Introduction: Prevention strategies and detection of latent COVID-19 infections in oncology staff and oncologic patients are essential to prevent outbreaks in a cancer center. In this study, we used two statistical predictive models in oncology staff and patients from the radiotherapy area to prevent outbreaks and detect COVID-19 cases.

Methods: Staff and patients answered a questionnaire (electronic and paper surveys, respectively) with clinical and epidemiological information. The data was collected through two online survey tools: Real-Time Tracking (R-Track) and Summary of Factors (S-Facts). According to the algorithm's models, cut-off values were established. SARS-CoV-2 qRT-PCR tests confirmed the algorithm's positive individuals. …

Effect Of Igf-1 On Post-Translational Modifications (Ptms) On A Model Of Diabetes-Induced Cardiac Hypertrophy In H9c2 Cardiomyoblast Cell Line, Andres Medina, Lizbeth Trevino, Alejandra Salinas, Yadira Chaires, Erick Rodriguez

Research Symposium

Background: Diabetic cardiomyocytes alter their post-translational modification levels, especially in OGlcNAcylation and Phosphorylation. Insulin-like growth factor 1 (IGF-1) is a peptide known to induce favorable cardiovascular effects in patients with heart failure. Here, we focus on the downstream effects of IGF-1 as a potential DCM treatment.

Methods: H9c2 cells were cultured in DMEM-10% FBS at 80% of confluence. As a cellular model of cardiac hypertrophy, we used a high-glucose medium (30 mM glucose) in the presence or absence of 10 µmol/L of IGF-1 (HG and HG+IGF-1). As control groups, we used cells cultured in low-glucose DMEM (glucose 5mM) in the …

Effect Of Chitosan And Hydroxypropyl Methylcellulose On Size And Antibacterial Activity Of Copper Nanoparticles, Tippabattini Jayaramudu, John Amalraj

Research Symposium

Background: Nanomaterials exhibit better antibacterial activity because of their distinct structural/ morphological characteristics. The particle size and shape of the nanomaterials are the two significant parameters which affect the resultant antibacterial property. Different biopolymers have been explored to prepare capped metal nanoparticles with controlled/desired particle size and morphology.

Methods: The present research work explains the effect of chitosan (CH) and hydroxypropyl methylcellulose (HPMC) on the shape and size of the copper nanoparticles (Cu NPs) and their antibacterial activity. The CH-Cu and HPMC-Cu NPs were achieved by facile precipitation technique using ascorbic acid as a nucleating agent.

Results …

Dosimetric And Volumetric Analysis In Endobronchial Brachytherapy Treatment Of Carcinoma Lung Patients: A Pilot Study, Ravi Kant, Meenu Gupta, Jyoti Bisht, Vipul Nautiyal, Viney Kumar, Rishabh Dobhal, Mushtaq Ahmed, Sunil Saini

Research Symposium

Background: To analyze the radiation doses received by Organ at Risk (OAR) nearby the target volume and volumetric changes in the target volume in carcinoma lung patients irradiated in three treatment session of endobronchial brachytherapy (EBBT).

Methods: Dosimetric analysis was conducted on patients of carcinoma Lung received three session of Endobronchial brachytherapy treatment in High Dose Rate brachytherapy unit with Ir-192 source. A flexible Lumencare catheter was inserted into the bronchus and positioned catheter tip at the tumor. Length of the implanted catheter was measured with the source position simulator device. Acquired three dimensional CT image data set with …

Differential Gene Expression Between African American And Caucasian American Prostate Cancer, Daniel L. Shen, Gregory T. Maclennan, Sanjay Gupta

Research Symposium

Background: African-American (AA) men have higher incidence and mortality from prostate cancer compared to Caucasian-American (CA) men. Increasing evidence suggests that genetic and molecular alterations play important roles. We identified a 5 gene panel viz. p-Akt (Ser473), chemokine (C-X-C motif) receptor 4 (CXCR4), fatty acid synthase (FASN), interleukin-6 (IL-6) and matrix metallopeptidase 9 (MMP-9) highly expressed in prostate cancer and analyzed their expression in AA and CA cohorts.

Methods: IHC of p-Akt, CXCR4, FASN, IL-6 and MMP-9 were evaluated in RRP specimens (n=20) from each ethnic group exhibiting Gleason scores ranging from 6 through 9.

Results: Low to medium staining …

Definitive Chemoradiation In Non Metastatic Squamous Cell Carcinoma Anal Canal: A Single Institution Experience, Viney Kumar, Saurab Bansal, Amit Badola, Vipul Nautiyal, Meenu Gupta, Mushtaq Ahmad, Sunil Saini

Research Symposium

Background: To analyze the oncological outcomes in anal canal squamous cell carcinoma treated with concurrent chemoradiotherapy.

Materials and Methods: A single centre retrospective hospital based study with sample size of 51 patients of anal canal Squamous cell carcinoma treated with concurrent chemoradiotherapy with mitomycin @10mg/m² and 5FU based. Disease free survival (DFS), Colostomy free survival (CFS) and Overall survival (OS) rates were calculated by Kaplan-Meier method.

Results: Among 51 eligible patients, after a median follow up of 46 months (range 10-68 months). The 3year Disease free survival (DFS) was 73.9%. 3patients developed locoregional recurrence while 1patient developed distant …

Development And Evaluation Of A Nanofiber Membrane In Vitro As A Therapeutic Alternative For The Post Treatment In Breast Cancer Cell In A Murine Model, Bruno A. Valades-Aguilar, Diana Ginette Zarate-Triviño, José Raúl Rángel-López, Moises Armides Franco-Molina, Jorge Luis Menchaca-Arredondo, María Cristina Rodríguez-Padilla

Research Symposium

Worldwide female breast cancer is the most commonly diagnosed cancer, with an estimated 2.3 million new cases in 2020, reason for the need of targeted therapies that can maximize treatment success and minimize toxicity. Nanoparticles of gold (AuNps) exhibit cytotoxic properties against certain types of cancer cell lines. Nanofibers have been use in the drug delivery systems due to its degradability and high surface area. We proposed a membrane with nanometric fibers using polivinilic alcohol (PVA) and chitosan (Qts) loaded with AuNps and Doxorubicin (Doxo) with the purpose of diminish tumor regression.

PVA-Qts membrane was develop with electrospinning, the injection, …

Developing An Assay For Easy And Rapid Detection Of Als Biomarker(S): A Hypothesis, Swati Dhasmana, Anupam Dhasmana, Meena Jaggi, Murali M. Yallapu, Subhash C. Chauhan

Research Symposium

Background: Death of motor neurons is the key pathology underlying neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). Biomarkers are chemical changes in the biological fluids. Biomarkers serving as a diagnostic tool should be specific to the concerned disease. Biomarkers indicating disease progression should be very sensitive to demonstrate changes during the disease process as well as therapeutics development. Biomarkers proposed for ALS include poly (GP) repeats in C9orf72, neurofilaments, miRNAs, glutathione and 4HNE in CSF, SOD1/TDP43 protein, poly (GP) repeats in C9orf72, neurofilaments, T regulatory cells, CRP, chitotriosidase, creatinine, creatinine kinase, miRNAs, glutamate, albumin, uric acid, glutathione, ferritin, …

Cyclophosphamide And Epirubicin Induce Apoptotic Cell Death In Microglia Cells, Rafael De La Hoz-Camacho

Research Symposium

Background. Chemotherapy Related Cognitive Impairment’s (CRCI), diminish patient’s quality life, being breast cancer (BC) patients the most affected. Microglia is described to play a major role in CRCI; hence, the aim of this research was to describe the cytotoxicity of cyclophosphamide (CTX) and Epirubicin (EPI), on microglia (SIM-A9), compared to BC cells (4T1).

Methods. We assessed cell viability (Resazurin) and cell death (AnnV), as well as nuclear damage with γ-H2AX, p53, p16 and cell cycle analysis (PI staining) by flow cytometry (FC). Furthermore, we evaluated ΔΨm (DIOC6), ROS (DCFDA) and NO (DAF-FM) production. Finally, caspase activation (TF2-VAD-FMK) and autophagy (CYTO-ID). …