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Full-Text Articles in Medicine and Health Sciences

Circulating Metabolic And Reproductive Hormone Changes In Laying Hens Kept Under Various Heat-Combating Systems, Muhammad Safdar Anjum, Mansur Abdullah Sandhu, Zia Ur-Rahman, Anum Safdar Jan 2016

Circulating Metabolic And Reproductive Hormone Changes In Laying Hens Kept Under Various Heat-Combating Systems, Muhammad Safdar Anjum, Mansur Abdullah Sandhu, Zia Ur-Rahman, Anum Safdar

Turkish Journal of Veterinary & Animal Sciences

The objective of the present study was to observe the circulating metabolic, reproductive, and stress hormones in laying hen performance under different heat-combating systems (HCSs). A total of 500 White Leghorn pullets at 24 weeks of age were subjected to four HCSs, i.e. desert cooling (DC), water sprinkling (WS), time limit feeding (TLF), and ascorbic acid (AA) supplementation, at environmental temperatures from 32.30 to 40.80 °C with 76.40% relative humidity. Blood was collected before heat stress, at peak stress, and after heat stress to collect plasma. The results of the 24-week stress period showed significantly less circulating T3, T4, estrogen, …


Effect Of Some Essential Oils (Allium Sativum L., Origanum Majorana L.) And Ozonated Olive Oil On The Treatment Of Ear Mites (Otodectes Cynotis) In Cats, Fulya Altinok Yi̇pel, Abuzer Acar, Mustafa Yi̇pel Jan 2016

Effect Of Some Essential Oils (Allium Sativum L., Origanum Majorana L.) And Ozonated Olive Oil On The Treatment Of Ear Mites (Otodectes Cynotis) In Cats, Fulya Altinok Yi̇pel, Abuzer Acar, Mustafa Yi̇pel

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the effect of certain essential oils and ozonated olive oil on Otodectes cynotis infestations in cats. Twenty-eight cats were included in the study. Infestation was diagnosed through the microscopic examination of ear secretions. Clinical findings were evaluated and scored before and after treatment. Cats were randomly assigned to different treatment groups, which included Group 1 treated with ozonated olive oil, Group 2 treated with garlic oil, Group 3 treated with marjoram oil, and Group 4 treated with permethrin. Each treatment was applied to both ears with approximately 5 drops daily per ear …


Age-Related Changes In The Growth Performance, Meat Quality, And Oxidativeprocesses In Breast Muscles Of Three Chicken Genotypes, Monika Michalczuk, Artur Józwik, Krzysztof Damaziak, Zaneta Zdanowska-Sasiadek, Agata Marzec, Dariusz Gozdowski, Nina Strzalkowska Jan 2016

Age-Related Changes In The Growth Performance, Meat Quality, And Oxidativeprocesses In Breast Muscles Of Three Chicken Genotypes, Monika Michalczuk, Artur Józwik, Krzysztof Damaziak, Zaneta Zdanowska-Sasiadek, Agata Marzec, Dariusz Gozdowski, Nina Strzalkowska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine growth performance, meat quality, and oxidative changes in breast muscles depending on the genotype and age of broiler chickens. The experiment was conducted on 1080 chickens: fast-growing Cobb 500, medium-growing Hubbard JA957, and a slow-growing experimental line. Chickens from all genetic groups were reared on litter for 8 weeks, but from the 5th week of age, 15 chickens from each group were slaughtered every week. After slaughter at weeks 5, 6, 7, and 8 of chickens? lives, their muscles were sampled for chemical composition, fatty acid profile, and cholesterol. Contents of reduced …


Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva Jan 2016

Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva

Turkish Journal of Veterinary & Animal Sciences

The purpose of this work was to study MspI polymorphism in the CAST gene in Soviet Merino and Salsk sheep breeds in the South European part of Russia and to find its relation with growth traits. Soviet Merino sheep have been found to have the M and N alleles with a frequency of 0.88 and 0.12 and the MM, MN, and NN genotypes with a frequency of 0.82, 0.12, and 0.06, respectively. Salsk sheep have been established to have the M and N alleles with a frequency of 0.89 and 0.11 and two genotypes, MM and MN, with a frequency …


Comparative Evaluation Of Sequence Analysis Of 16s Rrna And Rpobgenes For Identification Of Aquatic Mycobacteria, Fazel Pourahmad, Randolph Harvey Richards Jan 2016

Comparative Evaluation Of Sequence Analysis Of 16s Rrna And Rpobgenes For Identification Of Aquatic Mycobacteria, Fazel Pourahmad, Randolph Harvey Richards

Turkish Journal of Veterinary & Animal Sciences

The nucleotide sequences of partial 16S rRNA and bacterial RNA polymerase ß-subunit (rpoB) genes for 57 mycobacterial strains were determined. Compared to the 16S rRNA gene sequences, variable regions were scattered along the whole fragment sequence, indicating that the rpoB gene is more polymorphic. Unlike 16S rRNA sequences, species variation was observed within M. fortuitum strains. The topology of the rpoB-based phylogenetic tree was almost the same as that of the 16S rRNA sequence analysis. These results suggest that the rpoB gene is a highly conserved gene, and taxonomical studies based on this gene may be comparable with similar studies …


Isolation Of Virulent Rhodococcus Equi From Arabian Horses In Şanlıurfa, Turkey, Osman Yaşar Tel, Oktay Keski̇n, Sevi̇l Erdenli̇ğ Gürbi̇lek Jan 2016

Isolation Of Virulent Rhodococcus Equi From Arabian Horses In Şanlıurfa, Turkey, Osman Yaşar Tel, Oktay Keski̇n, Sevi̇l Erdenli̇ğ Gürbi̇lek

Turkish Journal of Veterinary & Animal Sciences

The purposes of this study were to investigate plasmid profiles of the isolated virulent (VapA positive) agents by RFLP and to determine the quantitative aspects of Rhodococcus equi in the feces of adult horses and foals, as well as in soil. The frequency of detection of R. equi and virulent R. equi in fecal samples and soil was studied from 20 farms in the Şanlıurfa region. A total of 315 samples were examined. Nasal swabs from 25 foals with pneumonia were also analyzed. R. equi was isolated and identified from 263 (83.4%) samples originating from 15 out of 20 farms. …


Molecular Characterization Of The Bovine Noroviruses From Diarrheic Calves In Turkey, İrem Gülaçti, İbrahi̇m Sözdutmaz, Hakan Işidan Jan 2016

Molecular Characterization Of The Bovine Noroviruses From Diarrheic Calves In Turkey, İrem Gülaçti, İbrahi̇m Sözdutmaz, Hakan Işidan

Turkish Journal of Veterinary & Animal Sciences

In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase (RdRp) gene. The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR. The nucleotide sequences of partial RdRp fragments from the BNoV isolates including the newly identified Turkish isolates showed more than 86.3% nt and 96.8% aa identity with each other. Interestingly, in a comparison of the nt and …


Growth, Slaughter, And Carcass Characteristics Of Honamlı, Hair, And Honamlı X Hair (F1) Male Goat Kids Bred Under Extensive Conditions, Aykut Asim Akbaş, Mustafa Saatci Jan 2016

Growth, Slaughter, And Carcass Characteristics Of Honamlı, Hair, And Honamlı X Hair (F1) Male Goat Kids Bred Under Extensive Conditions, Aykut Asim Akbaş, Mustafa Saatci

Turkish Journal of Veterinary & Animal Sciences

Growth and carcass characteristics of Honamlı, Hair, and Honamlı x Hair crossbred goat kids were investigated in this study. Seven kids, having live weights between 25 and 30 kg, were slaughtered. It was found that the live weights of Honamlı, Hair, and Honamlı x Hair kids were 3.90 kg and 27.50 kg, 3.04 kg and 16.91 kg, and 3.58 kg and 22.07 kg at birth and on the 120th day, respectively. Honamlı kids had higher body measurements, as well as birth and live weights, when compared to the other genotypes. While flocks had an important effect (P < 0.001) on all growth periods, the effect of dam age on body measurements was also significant (P > 0.05), except on …


Influence Of Different Proteinaceous Diets On Consumption, Brood Rearing,And Honey Bee Quality Parameters Under Isolation Conditions, Abdulraouf Amro, Mohamed Omar, Ahmed Al-Ghamdi Jan 2016

Influence Of Different Proteinaceous Diets On Consumption, Brood Rearing,And Honey Bee Quality Parameters Under Isolation Conditions, Abdulraouf Amro, Mohamed Omar, Ahmed Al-Ghamdi

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to evaluate the efficacy of 5 proteinaceous diets on honey bees under isolation conditions. The diets were soybean meal, mesquite pod powder, date paste, Feedbee®, and corn gluten, along with control colonies (bees fed naturally on pollens). These 5 diets were supplied to honey bee colonies under isolation conditions, except for the control group. Food consumption amount, brood rearing activity, and some physiological parameters of the honey bees were measured. The best consumption amount was for date paste, followed by Feedbee, mesquite, and corn gluten, respectively. Concerning brood rearing activity, the highest mean of sealed …


Effect Of Different Extenders On Motility And Some Sperm Kinematics Parameters In Norduz Goat Semen, Koray Teki̇n, Ali̇ Daşkin Jan 2016

Effect Of Different Extenders On Motility And Some Sperm Kinematics Parameters In Norduz Goat Semen, Koray Teki̇n, Ali̇ Daşkin

Turkish Journal of Veterinary & Animal Sciences

Use of computer-assisted sperm analyzers (CASAs) to evaluate sperm fertilizing ability has great importance for the artificial insemination industry. For motility evaluation, different CASA systems and diluents are being used. These assays are poor in predicting sperm fertility, because only the samples with markedly poor quality can be detected. However, there is not a unitary extender worldwide. Therefore, we aimed to evaluate the postthaw kinetic parameters of curvilinear velocity (VCL), straight-line velocity (VSL), and average-path velocity (VAP) with two different extenders (skim milk, SM; Tris, TS). Semen was collected twice a week with an artificial vagina from four adult Norduz …


The Role Of Porcine Reproductive And Respiratory Syndrome Virus As A Risk Factor In The Outbreak Of Porcine Epidemic Diarrhea In Immunized Swine Herds, Mei-Zhou Huang, Hui Wang, Sheng-Yi Wang, Dong-An Cui, Xin Tuo, Yong-Ming Liu Jan 2016

The Role Of Porcine Reproductive And Respiratory Syndrome Virus As A Risk Factor In The Outbreak Of Porcine Epidemic Diarrhea In Immunized Swine Herds, Mei-Zhou Huang, Hui Wang, Sheng-Yi Wang, Dong-An Cui, Xin Tuo, Yong-Ming Liu

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to investigate the relation between porcine epidemic diarrhea (PED) and porcine reproductive and respiratory syndrome virus (PRRSV). Here, we constructed a dynamic clinical experimental model in which sows were infected with PRRSV. The stability of the experimental model was surveyed by detecting the levels of antibodies against classical swine fever virus (CSFV) or PRRSV, respectively. In both group A (infected group) and group B (healthy group), the CSFV antibody level was not significantly different (P > 0.05). In group B, the PRRSV antibody level was also not significantly different (P > 0.05) at the three times …


The Effect Of Packaging Method And Dietary Vitamin D3 Supplementation On The Quality Of Beef In Rectus Femoris, Gluteus Medius, And Adductor Femoris Beef Muscles, Aleksandra Lipinska, Andrzej Póltorak, Agnieszka Wierzbicka, Jaroslaw Wyrwisz Jan 2016

The Effect Of Packaging Method And Dietary Vitamin D3 Supplementation On The Quality Of Beef In Rectus Femoris, Gluteus Medius, And Adductor Femoris Beef Muscles, Aleksandra Lipinska, Andrzej Póltorak, Agnieszka Wierzbicka, Jaroslaw Wyrwisz

Turkish Journal of Veterinary & Animal Sciences

The goal of the study was to assess the impact of packaging methods and doses of vitamin D3 fed to animals (with feed) in the last days of finishing on selected indicators of beef quality. The parameters studied include the measurement of CIE L*, a*, and b* colour spaces and the Warner?Bratzler shear force. The muscles used in the study were the m. rectus femoris, m. gluteus medius, and m. adductor femoris, which were packed in two variants of modified atmosphere (MAP1, 80% O2:20% CO2 and MAP2, 60% O2:40% CO2) and in skin-pack technology; they were later aged for 10 …


The Effect Of Live Saccharomyces Cerevisiae Yeast In The Diet Of Rams On The Digestibility Of Nutrients, Nitrogen And Mineral Retention, And Blood Serum Biochemical Parameters, Barbara Kowalik, Jacek Skomial, Renata Miltko, Malgorzata Majewska Jan 2016

The Effect Of Live Saccharomyces Cerevisiae Yeast In The Diet Of Rams On The Digestibility Of Nutrients, Nitrogen And Mineral Retention, And Blood Serum Biochemical Parameters, Barbara Kowalik, Jacek Skomial, Renata Miltko, Malgorzata Majewska

Turkish Journal of Veterinary & Animal Sciences

The effect of live Saccharomyces cerevisiae yeast added to the diet of rams on the digestibility of carbohydrates and protein in the total tract, retention of nitrogen and mineral elements, and concentration of biochemical parameters in blood serum was examined. The experiment was carried out on 12 Corriedale rams allocated to 2 groups of 6 animals each. The control group was fed meadow hay (45.4%), concentrate (53.2%), and a vitamin?mineral mixture (1.4%), while the second group was fed the same diet supplemented with 2 g day-1 live S. cerevisiae. Adaptation to feeding lasted 21 days; the sampling period lasted 7 …


Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams Jan 2016

Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams

Turkish Journal of Veterinary & Animal Sciences

Tilmicosin (TLM) is a semisynthetic antimicrobial agent used mainly in poultry and cattle, but it has relatively poor oral bioavailability. This study was conducted to compare the bioavailability (BA) and main pharmacokinetic (PK) parameters of TLM after oral administration of tilmicosin phosphate (TLM-PH) and three newly prepared lipid nanoparticles (LNPs) of TLM including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Sixty broiler chickens were divided into eight groups. In four treatment groups (n = 10), each bird was given a single oral dose (20 mg/kg) of a TLM formulation after overnight fasting, and in four …


Hemopoiesis In The Pronephros Of Tench, Tinca Tinca, Linnaeus 1758(Teleostei, Cyprinidae): Cytochemical Identification And Cell Morphology, Andi Alijagic, Damir Suljevic Jan 2016

Hemopoiesis In The Pronephros Of Tench, Tinca Tinca, Linnaeus 1758(Teleostei, Cyprinidae): Cytochemical Identification And Cell Morphology, Andi Alijagic, Damir Suljevic

Turkish Journal of Veterinary & Animal Sciences

Hemopoiesis in the tench (Tinca tinca: Teleostei, Cyprinidae), a cyprinid fish that can be found within the entire territory of Eurasia, from France in the west to the Yenisei River in the east, has not yet been described. The aim of this research was analysis of hemopoiesis lineages in the pronephros of Tinca tinca (sampled from the Jablanicko Reservoir). Identification and cells measurements were done by using a light microscope (Olympus BX41) and an Olympus DP12 camera. Hemopoietic cells of the tench are divided into five different series that include erythropoietic, granulopoietic, lymphopoietic, thrombopoietic, and monopoietic cell series. Erythropoietic series …


The Protective Effects Of Cysteamine, Putrescine, And The Combination Of Cysteamineand Putrescine On Fibrosarcoma Induced In Mice With 3-Methylcholanthrene, Abdullah Doğan, Pinar Aksu, Di̇nçer Erdağ, Ali̇ Nazmi̇ Can Doğan, Kadi̇r Özcan, Ertan Doğan Jan 2016

The Protective Effects Of Cysteamine, Putrescine, And The Combination Of Cysteamineand Putrescine On Fibrosarcoma Induced In Mice With 3-Methylcholanthrene, Abdullah Doğan, Pinar Aksu, Di̇nçer Erdağ, Ali̇ Nazmi̇ Can Doğan, Kadi̇r Özcan, Ertan Doğan

Turkish Journal of Veterinary & Animal Sciences

In this study, the protective effects of cysteamine, putrescine, and the combination of cysteamine and putrescine were investigated in mice with fibrosarcoma induced with 3-methylcholanthrene (3-MC). A total of 135 adult male albino mice (Mus musculus), 2-3 months old and weighing 20 ± 2.0 g, were used in this study. All groups (each consisting of 15 mice) were fed ad libitum. The experimental groups were Group 1 (drinking water), Group 2 (0.2 mL sesame oil s.c.), Group 3 (1 mg 3-MC/0.2 mL sesame oil), Group 4 (0.1% cysteamine), Group 5 (0.1% putrescine), Group 6 (0.1% cysteamine + putrescine), Group 7 …


Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam Jan 2016

Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of diet and exogenous enzymes on growth responses and micronutrient digestibility of broilers. Day-old broilers (n = 256) were distributed into a 2 x 2 factorial design and were fed on two basal diets: soybean (T1) or canola (T2) meals as such, or supplemented with enzymes up to 21 days. Feed intake (FI) and live weight (LW) to 21 days on the T2 diet was higher (P < 0.001) than that on T1. Feed conversion ratio (FCR) was better (P < 0.01) on T1 than on T2 at 21 days. Enzymes generally improved (P < 0.001) FI, FCR, and LW. The digestibility of histidine was higher (P < 0.05) on T2, but lysine digestibility was higher (P < 0.01) on T1 at 21 days. Histidine, threonine, lysine, valine, isoleucine, and phenylalanine digestibility was improved (P < 0.05) in the supplemented diets. Mineral digestibility was unaffected (P > 0.05) by diet, but enzymes increased (P < 0.05) digestibility of P, K, Mn, and Cu at 21 days. The digestibility of Cu, Zn, and Mg was higher (P < 0.05) on T2, whereas Ca digestibility was greater (P < 0.05) on the T1 diet. Broilers' growth responded positively to enzyme diets, probably due to improvement in nutrient digestibility.


Differences In Drone Sperm Morphometry And Activity At The Beginning And End Of The Season, Aldona Gontarz, Dorota Banaszewska, Magdalena Gryzinska, Katarzyna Andraszek Jan 2016

Differences In Drone Sperm Morphometry And Activity At The Beginning And End Of The Season, Aldona Gontarz, Dorota Banaszewska, Magdalena Gryzinska, Katarzyna Andraszek

Turkish Journal of Veterinary & Animal Sciences

The sperm cells of the honey bee, like the sperm of most invertebrates, belong to the modified type of spermatozoa. In our own research, differences have been observed in the activity of sperm cells isolated from drones at the beginning and end of the season. On the basis of these observations, research was undertaken to determine whether the changes in drone activity are associated with morphological changes. The sperm cells collected from drones at the beginning of the mating season are longer than the sperm cells from the end of the season. Moreover, sperm cells from the beginning of the …


In Vitro Culture Of In Vivo Saanen Goat Embryos By Vitrification, Ayşe Merve Köse, Tevfi̇k Tekeli̇ Jan 2016

In Vitro Culture Of In Vivo Saanen Goat Embryos By Vitrification, Ayşe Merve Köse, Tevfi̇k Tekeli̇

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to investigate the survival rate of high-quality embryos (n: 101) derived in vivo from Saanen goats (n: 15) in culture following their vitrified freezing and thawing during the breeding season. The in vitro postthaw survival rates of 101 vitrified embryos at 24, 48, and 72 h were 59.4%, 33.6%, and 25.7%, respectively. According to the developmental stage of the embryos, the survival rates were 51.3%, 20.5%, and 15.4% in the morula, and 64.5%, 41.9%, and 32.3% in the blastocyst, respectively. The survival rates of the blastocysts and morulae at 48 and 72 h were …


Investigation Of The Promoter Polymorphisms Of The Growth Hormone (Gh1), Growth Hormone Receptor (Ghr), Insulin-Like Growth Factor (Igf-I), And Prolactin (Prl) Genes And The Correlation Between Gene Expression And Milk Yields In Holstein Cattle Raised In Central Anatolia, Korhan Arslan, Serpi̇l Taheri̇, Eli̇f Funda Şener, Bi̇lal Akyüz, Aytaç Akçay, Yusuf Özkul, Kaan Muhsi̇n İşcan Jan 2016

Investigation Of The Promoter Polymorphisms Of The Growth Hormone (Gh1), Growth Hormone Receptor (Ghr), Insulin-Like Growth Factor (Igf-I), And Prolactin (Prl) Genes And The Correlation Between Gene Expression And Milk Yields In Holstein Cattle Raised In Central Anatolia, Korhan Arslan, Serpi̇l Taheri̇, Eli̇f Funda Şener, Bi̇lal Akyüz, Aytaç Akçay, Yusuf Özkul, Kaan Muhsi̇n İşcan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the promoter polymorphisms of the growth hormone (GH1), growth hormone receptor (GHR), insulin-like growth factor (IGF-I), and prolactin (PRL) genes, and the correlation of the expression levels of these genes with lactation and daily milk yields in Holstein cattle. A total of 154 lactating Holstein cows raised in the Central Anatolia Region of Turkey, with a mean age of 5.4 years and a mean body weight of 571.7 kg, constituted the study material. Each blood sample was examined by real-time PCR for mRNA expression levels and the promoter polymorphisms of the GH1, …


Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r Jan 2016

Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates …


Molecular And Seroprevalence Of Canine Visceral Leishmaniasis In West Anatolia, Turkey, Serkan Bakirci, Hüseyi̇n Bi̇lgi̇n Bi̇lgi̇ç, Onur Köse, Ayça Aksulu, Seli̇n Hacilarlioğlu, Hasan Erdoğan, Tüli̇n Karagenç Jan 2016

Molecular And Seroprevalence Of Canine Visceral Leishmaniasis In West Anatolia, Turkey, Serkan Bakirci, Hüseyi̇n Bi̇lgi̇n Bi̇lgi̇ç, Onur Köse, Ayça Aksulu, Seli̇n Hacilarlioğlu, Hasan Erdoğan, Tüli̇n Karagenç

Turkish Journal of Veterinary & Animal Sciences

Canine leishmaniasis (CanL) is a protozoal disease caused by Leishmania species. Dogs play an important role as a reservoir. The aim of this study is to determine the prevalence of CanL by molecular and serological techniques among stray dogs living in kennels in Aydın, Manisa, and İzmir, located in western Turkey. Blood samples were collected from a total of 191 dogs and analyzed using indirect immunofluorescence antibody test (IFAT) and polymerase chain reaction (PCR). The isolates that tested positive using PCR were further analyzed by real-time PCR for species identification and confirmation. A total of 21 (10.99%) dogs were found …


Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum Jan 2016

Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum

Turkish Journal of Veterinary & Animal Sciences

This cross-sectional study was conducted in and around Addis-Zemen from November 2014 to April 2015 to determine the prevalence of ovine coccidiosis and to identify associated risk factors. Faecal samples were collected directly from the rectum of 384 randomly selected animals. A centrifugal faecal flotation technique using saturated salt solution was used to detect Eimeria oocysts. Out of 384 samples, only 88 were found to contain Eimeria oocysts. Eimeria infection was recorded in all age and sex groups of the study animals. A strong significant association was observed (P < 0.05) between Eimeria infection and the faecal consistency, production system, hygienic status, age, and body condition of the sampled animals. However, an insignificant (P > 0.05) association was recorded between Eimeria infection and the origins and …


The Effects Of Carvacrol And/Or Thymol On The Performance, Blood And Rumen Parameters, And Carcass Traits Of Merino Sheep, Hakan Bi̇ri̇ci̇k, Hülya Hanoğlu Oral, Ali̇ Mehmet Taluğ, Şeri̇fe Şule Cengi̇z, Mehmet Koyuncu, Serdal Di̇kmen Jan 2016

The Effects Of Carvacrol And/Or Thymol On The Performance, Blood And Rumen Parameters, And Carcass Traits Of Merino Sheep, Hakan Bi̇ri̇ci̇k, Hülya Hanoğlu Oral, Ali̇ Mehmet Taluğ, Şeri̇fe Şule Cengi̇z, Mehmet Koyuncu, Serdal Di̇kmen

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effects of increasing doses of carvacrol (C) and/or thymol (T) on the performance, rumen fermentation, and blood and carcass parameters of Merino sheep. Eighty-four 12-week-old male Merino lambs were randomly assigned to 7 treatment groups. The sheep were fed with the same concentrate mixtures including a control diet, carvacrol 100 mg/kg (C100), carvacrol 300 mg/kg (C300), thymol 100 mg/kg (T100), thymol 300 mg/kg (T300), carvacrol+thymol 100 mg/kg (C50+T50), and carvacrol+thymol 300 mg/kg (C150+T150). The C and/or T supplementation did not affect the feed conversion. The lambs fed with C and/or T …


Egg Production, Egg Quality, And Cecal Microbial Populations Of Layersfed Diets Supplemented With Fermented Phytogenic Feed Additive, Jae Hong Park, Tae Hwa Song, Inho Kim Jan 2016

Egg Production, Egg Quality, And Cecal Microbial Populations Of Layersfed Diets Supplemented With Fermented Phytogenic Feed Additive, Jae Hong Park, Tae Hwa Song, Inho Kim

Turkish Journal of Veterinary & Animal Sciences

This study was undertaken to examine the effect of fermented phytogenic feed additive (FPFA) containing Gynura procumbens and Rehmannia glutinosa on egg production, egg quality, and cecal microflora population in layers. In total, 288 Hy-Line brown layers were randomly divided into 4 dietary treatment groups with 6 replicate pens per treatment group for 5 weeks. The dietary treatments were the control group (CON; basal diet without FPFA), FPFA1 (CON + 0.5% FPFA), FPFA2 (CON + 1% FPFA), and FPFA3 (CON + 2% FPFA). Egg production and egg weight during the experiment (35-39 weeks) improved linearly with the supplementation of 1% …


Serological Survey For Antibodies Against Mycobacterium Avium Complexin Hunting Dogs In East-Central Portugal, Ana Claudia Coelho, Manuela Matos, Luis Figueira, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Catarina Matos Jan 2016

Serological Survey For Antibodies Against Mycobacterium Avium Complexin Hunting Dogs In East-Central Portugal, Ana Claudia Coelho, Manuela Matos, Luis Figueira, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Catarina Matos

Turkish Journal of Veterinary & Animal Sciences

The Mycobacterium avium complex (MAC) causes infections and diseases in humans, birds, livestock, and wildlife, among others. In dogs, MAC infection is not frequent; however, due to its potential zoonotic characteristics, mainly in immunocompromised patients, the study of MAC epidemiology is necessary. The aim of this work was to perform a study to detect the presence of antibodies against MAC in serum samples of 53 hunting dogs in the Idanha-a-Nova municipality of East-Central Portugal. A total of 23 dogs were seropositive by commercial ELISA test (43.4%, 95% CI: 30.1%-56.7%). Factors such as age, breed, and sex were not significant. The …


Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş Jan 2016

Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …


The Effect Of Feeding Rhizopus Oligosporus On Growth Performance, Nutrient Digestibility, Blood Profile, Fecal Microbiota, And Fecal Score In Weanling Pigs, Jae Hong Park, Hyeok Min Yun, In Ho Kim Jan 2016

The Effect Of Feeding Rhizopus Oligosporus On Growth Performance, Nutrient Digestibility, Blood Profile, Fecal Microbiota, And Fecal Score In Weanling Pigs, Jae Hong Park, Hyeok Min Yun, In Ho Kim

Turkish Journal of Veterinary & Animal Sciences

Piglets were allocated to 1 of 3 treatments by body weight and sex, and fed different levels of 0%, 0.15%, and 0.30% of R. oligosporus 1.0 x 106 cfu/g, in a basal diet based on corn-soybean meal for 35 days. Piglets fed diets supplemented with R. oligosporus had higher average daily gain during days 15-35 and 1-35 (quadratic, P < 0.05). Feed conversion ratio during 1-35 days showed a significant improvement, as dietary R. oligosporus supplementation increased from 0.15% to 0.30% (linear, P < 0.05). Apparent total tract digestibility (ATTD) of crude protein (CP) was increased at 2 weeks, as dietary R. oligosporus supplementation increased significantly (linear and quadratic, P < 0.05), and ATTD of dry matter, CP, and energy were linearly increased at 5 weeks (P < 0.05). The serum IgG concentration was significantly increased in the R. oligosporus-fed groups (quadratic, P < 0.05). Fecal Lactobacillus counts were significantly increased quadratically and coliform bacteria counts were decreased linearly, as dietary R. oligosporus supplementation increased (P < 0.05). In conclusion, diets containing R. oligosporus may improve the growth performance, nutrient digestibility, IgG concentration, and intestinal environment in weanling pigs.


The Determination Of In Vitro Gas Production And Metabolizable Energy Value Of Rice Straw Treated With Exogenous Fibrolytic Enzymes, Zehra Selçuk, Nurcan Çeti̇nkaya, Mustafa Salman, Buğra Genç Jan 2016

The Determination Of In Vitro Gas Production And Metabolizable Energy Value Of Rice Straw Treated With Exogenous Fibrolytic Enzymes, Zehra Selçuk, Nurcan Çeti̇nkaya, Mustafa Salman, Buğra Genç

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effects of cellulase and xylanase treatments on in vitro 24-h gas production (GP), organic matter digestibility (OMD), and metabolizable energy (ME) values of rice straw (RS). Rice straws were treated with cellulase and xylanase at the levels of 0% (C1), 0.5% (RS+CEL0.5), 1% (RS+CEL1), 1.5% (RS+CEL1.5), and 2% (RS+CEL2) and 0% (C2), 0.5% (RS+XYL0.5), 1% (RS+XYL1), 1.5% (RS+XYL1.5), and 2% (RS+XYL2), respectively. Rice straws treated with each level of cellulase and xylanase were ensiled in 6 glass jars. For each level of cellulase and xylanase treatment, 3 glass jars were incubated at room …


Effects Of Dietary Chicken Grill And Sunflower Seed Oils On Performance, Egg Yolk Cholesterol Level, Biochemical Parameters, And Oxidant/Antioxidant Status Of Laying Hens, Mustafa Numan Oğuz, Fatma Karakaş Oğuz, Tülay Büyükoğlu Jan 2016

Effects Of Dietary Chicken Grill And Sunflower Seed Oils On Performance, Egg Yolk Cholesterol Level, Biochemical Parameters, And Oxidant/Antioxidant Status Of Laying Hens, Mustafa Numan Oğuz, Fatma Karakaş Oğuz, Tülay Büyükoğlu

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the effects of dietary chicken grill oil (CGO) and sunflower seed oil (SO) on performance, some serum and egg yolk biochemical parameters, and blood and liver oxidant/antioxidant status of laying hens. Fifty-four 19-week-old Lohmann laying hens were randomly assigned to three groups. The groups were fed experimental diets containing 5% SO, 5% CGO1, or 7.5% CGO2, respectively. Diets and water were provided ad libitum over 17 weeks. The feed efficiency ratios and cholesterol content in egg yolks with cholesterol and the triglyceride levels in serum were not affected by the diets. However, …