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Full-Text Articles in Medicine and Health Sciences

The Effect Of Esthetic Crown Lengthening On Perceptions Of A Patient’S Attractiveness, Friendliness, Trustworthiness, Intelligence, And Self-Confidence, Samuel Lyon Malkinson Apr 2012

The Effect Of Esthetic Crown Lengthening On Perceptions Of A Patient’S Attractiveness, Friendliness, Trustworthiness, Intelligence, And Self-Confidence, Samuel Lyon Malkinson

Theses and Dissertations

BACKGROUND: Smile esthetics play a major role in the perception of a person’s attractiveness, as well as other social parameters. The study aim was to see if altering the gingival display of patients would affect perceptions of the aforementioned social parameters. METHODS: Smiling photographs were taken and then digitally altered so as to lengthen the teeth and reduce the amount of gingiva. These photographs were shown to a group of senior dental students, and a group of evaluators with no formal dental training. Groups were asked to rate each picture’s attractiveness, friendliness, trustworthiness, intelligence, and self-confidence. RESULTS: The digitally altered …


Examining Racial Differences In Sympathetic Activity Assessed During Recovery From Exercise In Obese Adolescent Females, Stacey Hall Apr 2012

Examining Racial Differences In Sympathetic Activity Assessed During Recovery From Exercise In Obese Adolescent Females, Stacey Hall

Theses and Dissertations

Sympathetic overactivity (SO) is associated with obesity and cardiovascular disease (CVD) mortality. Black adolescents have an increased risk of obesity and CVD later in life, particularly females. PURPOSE: To evaluate differences in SO between black (BOA) and white obese female adolescents (WOA). METHODS: Sixty-one BOA (n=49, 13.7±1.6 yrs, 38.1±6.1 kg/m2) and WOA (n=12, 13.3±2.2 yrs, 34.3±4.9 kg/m2) completed a maximal graded treadmill test after which an exercise recovery index (ERI; heart rate/VO2 plateau) was calculated. RESULTS: The ERI was significantly greater in BOA compared to WOA (29.8 ± 6.4 vs. 24.1 ± 3.1, P = 0.004). Multiple linear regression modeling …


Accuracy Of Cone-Beam Computerized Tomography In Determining The Thickness Of The Palatal Masticatory Mucosa, Justin Hardison Apr 2012

Accuracy Of Cone-Beam Computerized Tomography In Determining The Thickness Of The Palatal Masticatory Mucosa, Justin Hardison

Theses and Dissertations

BACKGROUND: The aim was to compare the thickness of the palatal masticatory mucosa as determined on a cone-beam computerized (CBCT) scan versus thickness determined via bone-sounding. METHODS: A total of twenty patients requiring palatal surgery participated. Thickness of the palatal tissue was measured at various points radiographically and clinically. The two techniques were compared to determine the agreement of the two measurement modalities. RESULTS: Analysis of variance determined that there was no significant difference between the two methods. A small bias of the radiographic measurement being larger was found to be statistically significant (0.09 ± 0.69mm; p <0.0001). Moreover, the tissue thickness was shown to increase as the distance from the gingival margin increased and the tissue over the molars was thinner than the tissue over the premolars. CONCLUSIONS: CBCT can be used to accurately determine the soft tissue thickness of the palatal masticatory mucosa with minimal bias.


Tgf-Beta Receptors And Alcohol Sensitivity In Drosophila, Kristyn Sennett Apr 2012

Tgf-Beta Receptors And Alcohol Sensitivity In Drosophila, Kristyn Sennett

Theses and Dissertations

Clic proteins influence ethanol-related behavior in flies and other species and also mediate TGF-β signaling. These findings suggest that Clics and the TGF-β signaling pathway might work together to modulate behavioral responses to ethanol. I used the Drosophila model to address the hypothesis that TGF-β signaling is important for ethanol sensitivity. Ethanol sensitivity was blunted by multiple transposon insertions in the TGF-β receptor gene thickveins. Collectively, however, I found no consistent correlation between expression of thickveins and altered ethanol sensitivity in flies harboring transposons. I therefore also assessed ethanol sensitivity in flies with loss of function point mutations in thickveins. …


The Role Of Sulfatide In The Development And Maintenance Of The Nodal And Paranodal Domains In The Peripheral Nervous System, Heather Herman Apr 2012

The Role Of Sulfatide In The Development And Maintenance Of The Nodal And Paranodal Domains In The Peripheral Nervous System, Heather Herman

Theses and Dissertations

Sulfatide is a galactolipid and a major lipid component of the myelin sheath. Its production is catalyzed by the enzyme cerebroside sulfotransferase (CST). To determine the functions of sulfatide, the gene encoding CST was genetically disrupted resulting in mice incapable of sulfatide synthesis. Using these mice, it has been shown in the central nervous system (CNS) that sulfatide is essential for normal myelin synthesis and stability even though the onset of myelination is not impaired. Additionally, proper initial clustering of paranodal proteins and cluster maintenance of nodal proteins is impaired suggesting that paranodal domains are important for long-term node stability. …


Fullerene C70 Derivatives Dampen Anaphylaxis And Allergic Asthma Pathogenesis In Mice, Sarah Norton Apr 2012

Fullerene C70 Derivatives Dampen Anaphylaxis And Allergic Asthma Pathogenesis In Mice, Sarah Norton

Theses and Dissertations

Fullerenes are carbon nanospheres that can be solublized by the addition of polar chemical groups to the carbon cage, forming fullerene derivatives. One specifically derivatized fullerene compound, termed C70-Tetragylocolate (C70-TGA), has been shown to stabilize mast cell responses in vitro thus we hypothesized it may have an effect on mast cell-driven diseases such as asthma and systemic anaphylaxis. To observe the effects of C70-TGA on systemic anaphylaxis, mice were subjected to a model of passive systemic anaphylaxis. In this model, mice were injected with DNP-specific IgE 16 hours prior to challenge, then treated with C70-TGA. Immediately prior to DNP challenge, …


The Effect Of Oral Care On Intracranial Pressure In Critically Ill Adults, Christina Szabo Apr 2012

The Effect Of Oral Care On Intracranial Pressure In Critically Ill Adults, Christina Szabo

Theses and Dissertations

A major goal in the care of patients with neurological problems is to prevent or minimize episodes of increased intracranial pressure (ICP). Elevations in ICP in response to nursing interventions have been acknowledged since the 1960’s when ICP monitoring was first introduced in the clinical setting. Until recently few studies have specifically examined the effect of oral care on ICP and oral care and other hygiene measures were combined or not specified, prohibiting a direct interpretation of the influence of oral care alone on ICP. The purpose of this study was to describe the relationship between routine oral care interventions …


Hydrogen Sulfide As An Allosteric Modulator Of Atp Sensitive Potassium Channels In Colonic Inflammation., Aravind Gade Apr 2012

Hydrogen Sulfide As An Allosteric Modulator Of Atp Sensitive Potassium Channels In Colonic Inflammation., Aravind Gade

Theses and Dissertations

The ATP sensitive potassium channel (KATP) in mouse colonic smooth muscle cell is a complex containing a pore forming subunit (Kir6.1) and a sulfonyl urea receptor subunit (SUR2B). These channels are responsible for maintaining the cellular excitability of the smooth muscle cell which in turn regulates the motility patterns in the colon. We used whole-cell voltage-clamp techniques to study the alterations in these channels in smooth muscle cells in experimental model of colitis (colonic inflammation). Colitis was induced in BALB/C mice following an intracolonic administration of trinitrobenzene sulfonic acid (TNBS). KATP currents were measured at Vh -60 mV in high …


Retentive Cement Strengths With Passive Fit Primary Anterior Esthetic Crowns, Charles Davis Jr. Apr 2012

Retentive Cement Strengths With Passive Fit Primary Anterior Esthetic Crowns, Charles Davis Jr.

Theses and Dissertations

Purpose: to assess the retentive strengths of passive fit esthetic anterior restorations using three commercially available cements. Methods: Three resin dies were fabricated from the intaglio surface of each restoration type. Each die was prepared following the current accepted guidelines on primary anterior tooth crown preparation. The three prepared teeth were replicated to produce 30 dies for each of the three restoration types. The prepared teeth were further separated into nine groups of 10 teeth each. Thirty EZ Pedo Crowns, 30 NuSmile Primary Crowns and 30 Unitek crowns were cemented using hand pressure employing the luting cement assigned to the …


Effect Of Local Anesthesia On Postoperative Pain With General Anesthesia, Belinda Campbell Apr 2012

Effect Of Local Anesthesia On Postoperative Pain With General Anesthesia, Belinda Campbell

Theses and Dissertations

Purpose: The aim of this study was to determine if the use of local anesthesia with general anesthesia results in less postoperative pain. The alternative hypothesis is that children will experience less postoperative discomfort when utilizing intraligamental local anesthetic during the intra-operative time period. Methods: Patients were recruited for this single blind, randomized, prospective cohort study with the following inclusion criteria: English speaking children age 3-6 years, ASA I/II requiring general anesthesia for dental treatment. Randomization was done to place patients in groups of no local anesthetic vs. local anesthetic administration. A Wong-Baker Faces Pain Scale was utilized to evaluate …


Exploring The Social Determinants Of Sexually Transmitted Disease And High-Risk Sexual Behaviors, River Pugsley Apr 2012

Exploring The Social Determinants Of Sexually Transmitted Disease And High-Risk Sexual Behaviors, River Pugsley

Theses and Dissertations

Abstract 1: A multi-level assessment of disproportionate population sex ratios and high-risk sexual behaviors among STD clinic patients Low male-to-female sex ratios in a population may influence high-risk sexual behaviors, such as multiple sex partners and inconsistent condom use, which facilitate the transmission of STDs. This study used multi-level modeling to assess whether population sex ratios were associated with these two individual-level behaviors. All analyses were stratified by gender. Interview data were collected from patients (N = 9,203, 48% male) attending participating STD clinics. Interviews included information on socio-demographics and sexual behavior, including number of sex partners in the previous …


Workplace Discrimination And The Perception Of Disability, William Draper Apr 2012

Workplace Discrimination And The Perception Of Disability, William Draper

Theses and Dissertations

Decisions by the EEOC in favor of claimants perceived to have disabilities disproportionately exceeded those in favor of claimants with documented disabilities. This finding lends support to the assertion that unconscious/implicit bias is persistent in the workplace.


Assessing The Effects Of Stress Resilience Training On Visual Discrimination Skills: Implications For Perceptual Resilience In U.S. Warfighters, Andrea Taylor Apr 2012

Assessing The Effects Of Stress Resilience Training On Visual Discrimination Skills: Implications For Perceptual Resilience In U.S. Warfighters, Andrea Taylor

Theses and Dissertations

Current military operational environments are highly improvised and constantly evolving, threatening the lives of U.S. warfighters. For instance, since 2001, 60% of all hostile casualties and 65% of hostile injuries in the Middle East theater have been attributed to improvised explosive devices (IEDs). IEDs are powerful physical weapons, and the stressful atmosphere they, and other operational challenges create, can also result in a range of psychological dysfunctions, including anxiety, depression, alcohol abuse, and Post-Traumatic Stress Disorder (PTSD). Not only are these issues concerning for mental health reasons, they are also problematic in terms of combat performance. Extreme arousal (i.e., stress) …


Opioid Withdrawal Signs And Symptoms In The Pediatric Patient During Opioid Tapering, Deborah Fisher Apr 2012

Opioid Withdrawal Signs And Symptoms In The Pediatric Patient During Opioid Tapering, Deborah Fisher

Theses and Dissertations

Opioids are used routinely in the pediatric intensive care population for analgesia, sedation, blunting of physiologic responses to stress, and safety. In children, physical dependence may occur in as little as two to three days of continuous opioid therapy. Once the child no longer needs the opioid, the medications are reduced over time. A review of the literature revealed that the majority of the published studies used either a neonatal opioid assessment tool or no assessment tool. A subsequent international survey of pediatric providers found a wide range of opioid tapering practices and sporadic use of opioid withdrawal instruments to …


Overexpression Of Serum Response Factor In Astrocytes Improves Neuronal Plasticity In A Model Of Fetal Alcohol Spectrum Disorders, Arco P. Paul Apr 2012

Overexpression Of Serum Response Factor In Astrocytes Improves Neuronal Plasticity In A Model Of Fetal Alcohol Spectrum Disorders, Arco P. Paul

Theses and Dissertations

Neuronal plasticity deficits underlie many of the neurobehavioral problems seen in Fetal Alcohol Spectrum Disorders (FASD). Recently, we showed that third trimester alcohol exposure lead to a persistent disruption in ocular dominance (OD) plasticity. For instance, few days of monocular deprivation results in a robust reduction of cortical regions responsive to the deprived eye in normal animals, but not in ferrets exposed early to alcohol. This plasticity deficit can be reversed if alcohol-exposed animals are treated with a phosphodiesterase type 1 (PDE1) inhibitor during the period of monocular deprivation. PDE1 inhibition can increase cAMP and cGMP levels, activating transcription factors …


Myelin Gene Expression: Implications For Alcohol Abuse And Dependence, Sean Farris Mar 2012

Myelin Gene Expression: Implications For Alcohol Abuse And Dependence, Sean Farris

Theses and Dissertations

Acute behavioral responses to ethanol have predictive value for determining an individual's risk of long-term drinking behavior. Although the neurobiology of alcohol abuse is complex, prior studies from our laboratory demonstrated differential myelin-associated gene expression (MAGE) in medial prefrontal cortex (PFC) as one potential mechanism influencing acute ethanol behaviors between C57BL/6J (B6) and DBA/2J (D2) mice. Our laboratory and others have also shown MAGE is reduced in PFC of alcoholics. Herein, I have extended these findings through expression profiling of PFC into chronic models of ethanol self-administration from non-human primates and mice. Together, these results suggest that regulation of MAGE …


Antinociceptive Effects Of Monoamine Reuptake Inhibitors In Assays Of Pain-Stimulated And Pain-Depressed Behaviors, Marisa Rosenberg Mar 2012

Antinociceptive Effects Of Monoamine Reuptake Inhibitors In Assays Of Pain-Stimulated And Pain-Depressed Behaviors, Marisa Rosenberg

Theses and Dissertations

ANTINOCICEPTIVE EFFECTS OF MONOAMINE REUPTAKE INHIBITORS IN ASSAYS OF PAIN-STIMULATED AND PAIN-DEPRESSED BEHAVIOR By Marisa B. Rosenberg A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Virginia Commonwealth University. Virginia Commonwealth University, 2012 Advisor: Sidney Stevens Negus, Ph.D. Professor, Department of Pharmacology/Toxicology Noxious stimuli can produce pain-related stimulation of some behaviors (e.g. withdrawal responses) and depression of other behaviors (e.g. feeding, locomotion, responding maintained by many types of positive reinforcement). Monoamine reuptake inhibitors are used clinically to treat depression and to manage some types of pain. This study examined the antinociceptive properties …


Factors Associated With Access To Palliative Care In A Large Urban Public Teaching Hospital With A Formal Hospital-Based Palliative Care Program, Leland Waters Mar 2012

Factors Associated With Access To Palliative Care In A Large Urban Public Teaching Hospital With A Formal Hospital-Based Palliative Care Program, Leland Waters

Theses and Dissertations

Hospital-based specialist palliative care services are designed to address the needs of critically ill patients by psychosocial and spiritual support, improving symptoms management, and offering discussions on goals of care. Integrating palliative care upstream in the care continuum for patients who eventually die in the hospital will help to address the many individualistic needs of the critically ill patient. The diffusion of specialist hospital-based palliative care services requires an understanding of patterns of utilization by patients. The purpose of this study was to examine the population characteristics of decedents who may or may not have utilized specialist palliative care services …


A Cellular Automata Model Of Enantiomer Interactions With Beta-Cyclodextrin, Desoi Darren Mar 2012

A Cellular Automata Model Of Enantiomer Interactions With Beta-Cyclodextrin, Desoi Darren

Theses and Dissertations

The binding mechanisms of molecules to cyclodextrins continues to be studied to better explain the interactions occurring. The majority of published models focus on one-to-one molecular binding thermodynamics to explain experimental results. They rely on physical concepts of energies and forces to guide the actions of molecules expressed mathematically in terms of differential and non-linear equations. These models are limited in scope due to their complexity and are not easily expanded to study many diverse analytes. Conversely, cellular automata uses simple mathematical idealizations of systems governed by deterministic and probabilistic rules that are easily adaptable to many types of molecular …


Test-Retest Reliability In The Determination Of The Speech Recognition Threshold, Alyssa Montierth Jacobs Mar 2012

Test-Retest Reliability In The Determination Of The Speech Recognition Threshold, Alyssa Montierth Jacobs

Theses and Dissertations

For many years, speech recognition threshold (SRT) testing has been used as an indicator of audiologic health. However, with changing methods and technology, test-retest reliability has not been reviewed extensively with newer digitally recorded spondaic words which meet a published criterion of listener familiarity. This study examined the test-retest reliability of 33 high frequency usage and psychometrically equated spondaic words. The American Speech-Language-Hearing Association recommended method (2-dB decrements) was used to measure the left and right SRT of 40 participants using both male and female talker recordings. For each participant, four SRTs were found during the test condition and four …


Use Of Procalcitonin As A Biomarker Of Bacterial Infection In Acute Liver Failure And Acute Liver Injury, Jody Balko Mar 2012

Use Of Procalcitonin As A Biomarker Of Bacterial Infection In Acute Liver Failure And Acute Liver Injury, Jody Balko

Theses and Dissertations

Infections in patients with acute liver failure (ALF) and acute liver injury (ALI) are a frequent occurrence. Because ALF and ALI patients share many of the same clinical features as patients with severe sepsis and septic shock, identifying an infection based upon clinical manifestations is extremely difficult. Bacterial culture and sensitivity reports require 24 to 72 hours to be finalized after the need for a culture is suspected and obtained. During this time period, ALF and ALI patients are either not receiving required antibiotic therapy, receiving antibiotic therapy that is not required or not appropriate for the infecting bacterial pathogen, …


Probabilistic Model For Laser Damage To The Human Retina, David A. Wooddell Jr. Mar 2012

Probabilistic Model For Laser Damage To The Human Retina, David A. Wooddell Jr.

Theses and Dissertations

Understanding how lasers interact with media during propagation is a premiere field of physics. The subject area known as laser bioeffects explores laser interactions with biological cells, tissues, organs, and bodies. This research includes laser applications used in medicine, establishes safe exposure limits for industry and academia, and generally studies the many effects of laser light on living creatures. The bioeffects community relies heavily on deterministic modeling and simulation tools to support experimental research into damage thresholds and laser effects. However, recent laser applications require a probabilistic approach to support risk management and analyses methodologies. Some probabilistic models exist, but …


Comparative Analysis Of Two Biological Warfare Air Samplers Using Live Surrogate Agents, James C. Enderby Mar 2012

Comparative Analysis Of Two Biological Warfare Air Samplers Using Live Surrogate Agents, James C. Enderby

Theses and Dissertations

The United States Air Force has several high-volume biological air samplers, including the XMX/2L-MIL and the Biocapture 650. Limited information is available on either in terms of its collection of viruses and bacteria. However, previous research on the XMX/2L-MIL has determined that modifications to the secondary flow rate and the use of a virus preserving collection media may provide improved virus collection rates. In this thesis, these modifications were investigated to determine their impact on the collection of viral and bacterial aerosols. Additionally, relative collection rates were compared against those for the Biocapture 650. MS2 bacteriophage was the viral surrogate …


A Proposed Mediation Model Of The Effects Of Motivation For A Healthy Lifestyle: Impacts On Emotional Exhaustion, Medication Regimens, And Low-Density Lipoprotein, Nathan M. Leuthold Mar 2012

A Proposed Mediation Model Of The Effects Of Motivation For A Healthy Lifestyle: Impacts On Emotional Exhaustion, Medication Regimens, And Low-Density Lipoprotein, Nathan M. Leuthold

Theses and Dissertations

This research addressed the following question: "How are behavioral indicators related to physiological responses?" Three theories -- Conservation of Resources, Planned Behavior, and Achievement Motivation -- were employed to build a proposed model of the effects that an individual's motivation for a healthy lifestyle would have on his/her emotional exhaustion, daily medication usage, and low-density lipoproteins. Motivation for a healthy lifestyle was hypothesized to have a negative relationship with emotional exhaustion when using body mass index as a partial mediator. Emotional exhaustion was hypothesized to have positive relationships with daily medication usage and low-density lipoproteins. The model was tested using …


The Effects Of Cognitive-Behavioral Motivation For Health Improvement On Anthropometric Measurements In High Risk Individuals, Aimee T. Kirchner Mar 2012

The Effects Of Cognitive-Behavioral Motivation For Health Improvement On Anthropometric Measurements In High Risk Individuals, Aimee T. Kirchner

Theses and Dissertations

Unhealthy lifestyles cost businesses, governmental organizations, and the United States military billions of dollars every year. To fight this rising cost as well as potentially save lives this study sought to understand if a cognitive-behavioral motivation treatment could positively affect the cognitive variables (attitude, self-efficacy, and locus of control) that induce long term behavior change. Anthropometric measurements, specifically body mass index, abdominal circumference, and abdominal height, were used to determine if long term behavior change resulted from the treatment. The Theory of Planned Behavior was the basis of this thesis' model, while the Valence, Instrumentality, and Expectancy (VIE) theory was …


Influence Of Ph On The Transport Of Silver Nanoparticles In Saturated Porous Media: Laboratory Experiments And Modeling, Jason R. Flory Mar 2012

Influence Of Ph On The Transport Of Silver Nanoparticles In Saturated Porous Media: Laboratory Experiments And Modeling, Jason R. Flory

Theses and Dissertations

Given the ubiquity of silver nanoparticles (AgNPs), the largest and fastest growing category of nanomaterials, and their potential for toxic effects to both humans and the environment, it is important to understand their environmental fate and transport. The purpose of this study is to gain information on the transport properties of unmodified AgNP suspensions in a glass bead-packed column under saturated flow conditions at different solution pH levels. Commercial AgNPs were characterized using high resolution transmission spectroscopy (HRTEM), dynamic light scattering (DLS) and ultraviolet (UV) visible spectroscopy. Transport data were collected at different pH levels (4, 6.5 and 9) at …


Accuracy Of Automated Grammatical Tagging Of Narrative Language Samples From Spanish-Speaking Children, Tyson Gordon Harmon Mar 2012

Accuracy Of Automated Grammatical Tagging Of Narrative Language Samples From Spanish-Speaking Children, Tyson Gordon Harmon

Theses and Dissertations

The present study measured the accuracy of automated grammatical tagging software as compared to manual tagging in Spanish-speaking children's personal and fictional event narrative language samples. Studies have identified articles, clitic (contracted with a verb) pronouns, and verbs as clinical markers for language impairment in Spanish-speaking children. Automated grammatical tagging software may aid in the rapid identification of these grammatical markers. Grammatical morphemes of 30 first and fourth grade children's personal and fictional event narrative samples were tagged and compared with their respective manually tagged samples. The accuracy of word-level coding averaged 91%, and similar accuracy was found for clinically …


Elevating Endogenous Cannabinoids Reduces Opioid Withdrawal In Mice, Divya Ramesh Feb 2012

Elevating Endogenous Cannabinoids Reduces Opioid Withdrawal In Mice, Divya Ramesh

Theses and Dissertations

Delta9-tetrahydrocannbinol (THC), the primary active constituent of Cannabis sativa, has long been known to reduce opioid withdrawal symptoms. Although THC produces most of its pharmacological actions through the activation of CB1 and CB2 cannabinoid receptors, the role these receptors play in reducing opioid withdrawal symptoms remains unknown. The endogenous cannabinoids, N-arachidonoylethanolamine (anandamide; AEA) and 2-arachidonylglycerol (2-AG), activate both cannabinoid receptors, but are rapidly metabolized by fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL), respectively. The objective of this dissertation was to test whether increasing AEA or 2-AG, via inhibition of their respective hydrolytic enzymes, reduces morphine withdrawal symptoms in …


Comparison Of Steriplex™ Hc And Sodium Hypochlorite Cytotoxicity On Primary Human Gingival Fibroblasts, Jesse Harris Feb 2012

Comparison Of Steriplex™ Hc And Sodium Hypochlorite Cytotoxicity On Primary Human Gingival Fibroblasts, Jesse Harris

Theses and Dissertations

This study examined the cytotoxic effects of STERIPLEX™ HC (sBioMed, Orem, UT) and sodium hypochlorite (NaOCl) on human fibroblast cells in vitro. Fibroblasts exposed to various concentrations of NaOCl or STERIPLEX™ HC were visualized via light microscopy. Dilutions of either NaOCl or STERIPLEX™ HC that did not appear to disrupt the integrity of the cells were recorded for further analysis. Cells were then cultured and grown to confluence in five separate plates. A void was created down the middle of each plate. If the cells were viable, cellular confluence was seen. If nonviable, confluence of the cells did not occur. …


The Antimicrobial Effect Of Steriplex Hc In Comparison With Sodium Hypochlorite On Enterococcus Faecalis, Jonathan Coudron Feb 2012

The Antimicrobial Effect Of Steriplex Hc In Comparison With Sodium Hypochlorite On Enterococcus Faecalis, Jonathan Coudron

Theses and Dissertations

The study objective was to compare the antimicrobial activity of STERIPLEX™ HC with 5.25% sodium hypochlorite (NaOCl) at different dilutions (50%, 25%, 10%, 1%, 0.1%) and different time intervals (1, 3, 5 minutes) on Enterococcus faecalis. All data was analyzed using an ANOVA. The 50%, 25%, and 10% dilutions of both disinfectants reduced the colony forming unit (CFU) count to below the limit of detection (50 CFU/ml) after one minute. The 1% dilutions at each of the time intervals show NaOCl was significantly more effective than STERIPLEX™ HC (all Ps < .0001) in reducing the CFU/ml count. The 0.1% dilutions of NaOCl and STERIPLEX™ HC at 1 minute, were not different (P = 0.7808), while at 3 minutes and 5 minutes NaOCl was significantly more effective (P = 0.0098 and P < .0001, respectively).