Medicine and Health Sciences Commons^™

Dose-Dependent Effects Of Salmon Calcitonin On Bone Turnover In Ovariectomized Rats., Beatrice H. Owens

Electronic Theses and Dissertations

In the United States, osteoporosis results in about 1.5 million annual fractures, costing approximately $15 billion. Calcitonin is safe and effective in slowing osteoporotic bone loss, but its effect is transient. The current studies were designed to explore the dose-dependent effects of salmon calcitonin on bone turnover in ovariectomized rats and to determine if the decrease in therapeutic effectiveness of calcitonin demonstrated over time with higher doses is due to oversuppression of bone turnover. Doses of 5, 15, & 50 IU/kg BW/day of calcitonin were compared to placebo in 12-week-old ovariectomized and sham-ovariectomized Sprague-Dawley rats for 24 weeks. The spinal …

Childhood Experiences Of Appalachian Women Who Have Experienced Intimate Partner Violence During Adulthood., Amy L. Reeves

Electronic Theses and Dissertations

Recent national surveys in the United States estimate one in five females will experience abuse by an intimate partner during her lifetime. Previous quantitative research linked childhood victimization to repeated victimization in adult relationships. This qualitative study explored the lived experiences of childhood in eight female victims of intimate partner violence who were born and reared in southern Appalachia. Interviews were analyzed using a descriptive-interpretative phenomenological method, as described by Van Manen. The three essential themes from childhood were identified as: living 'as if' an orphan; surviving in chaos; and, manifesting a devalued self. These themes were congruent with findings …

Papain: A Novel Urine Adulterant., David Burrows

Electronic Theses and Dissertations

The estimated number of employees in the United Stated screened annually for illicit drugs is approximately 20 million, with marijuana being the most frequently abused drug. Urine adulterants provide an opportunity for illicit drug users to obtain a false negative result on commonly used primary drug screening methods such as the Fluorescence Polarized Immunoassay (FPIA) technique. Typical chemical adulterants such as nitrites are easily detected or render the urine specimen invalid as defined in the proposed federal guidelines for specimen validity testing based on creatinine, specific gravity and pH. Papain is a cysteine protease with intrinsic ester hydrolysis capability. The …

Early Speech And Language Development: A Comparison Of Typically Developing Children To Children With Cleft Palate., Holly Jannice Mcgahey

Electronic Theses and Dissertations

Previous research has shown that parent implemented intervention is effective in increasing the speech and language development of children with cleft lip and palate. To further determine the efficacy of this intervention, this study compared the speech and language development of children with CLP, who received parent implemented intervention, with a group of younger, typically developing children, matched for vocabulary size. This study also evaluated the language differences between the mothers of both groups of children. Speech and language assessments were administered to the typically developing children and their mothers at two times to mirror the time of assessment for …

Nongenomic Effects Of Estrogens On Epithelial Chloride Secretion., Sabyasachi Moulik

Electronic Theses and Dissertations

The human colonic cell line T84, a model for studying epithelial chloride secretion and cystic fibrosis chloride channel (CFTR) function, was used to investigate the regulatory role of estrogens in transepithelial ion transport. Estrogens and other steroid hormones do not stimulate chloride secretion by themselves. However, 17 β-estradiol (17β-E2) rapidly (within seconds to minutes) potentiates carbachol- and thapsigargin-stimulated chloride secretion measured as short circuit current in voltage-clamped T84 monolayer cultures. The cholinergic agonist carbachol and the SR Ca²⁺ ATPase inhibitor thapsigargin stimulate chloride secretion by elevating intracellular calcium. 17α-estradiol, a stereoisomer that does not activate nuclear estrogen receptors, is …

The Effects Of A Multimodality Approach On Sentence Production Using Response Elaboration Training With A Reading Component On Aphasic Patients., Sara E. Mccarthy

Electronic Theses and Dissertations

The effects of a multimodality treatment were investigated using a single subject experimental design across behaviors in two patients with different severity levels of Broca's aphasia. We hypothesized that Response Elaboration Training and an oral reading task would improve accuracy of sentence production, information content, and mean length of utterance. Results indicated that this treatment approach elicited significant improvement in the accuracy of sentence production and information content in the participant with very mild Broca's aphasia. Furthermore, the participant with severe Broca's aphasia demonstrated a very significant improvement in information content and mean length of utterance. The improvements support the …

Religion And Crime: A Study Of Inmates In State And Federal Prisons In The United States., Dhanalakshmi Thirumalai

Electronic Theses and Dissertations

Persistent high levels of crime in this country have provided support for the need for public programs that help in reducing crime rates through effective interventions. Spirituality and religion are considered to play a major part in determining an individual's attitude towards committing a crime. This study analyzed if inmates in State and Federal prisons in the US are involved in prison religious activities and personal prayers in order to determine if criminality (measured by offense type) of inmates affects their participation in religious activities. Based on logistic regression analysis, gender, race, and marital status of inmates were found to …

The Effect Of Closed Versus More Liberal Visitation Policies On Work Satisfaction Beliefs And Nurse Retention., Suzanne M. Boswell

Electronic Theses and Dissertations

Nurses believe that more liberal visitation policies will cause greater work overload and decrease the quality of patient care. The purpose of this study was to investigate the effect of a liberal visitation policy on work satisfaction, beliefs, and retention of nurses.

In this study, nurses were asked to complete a survey before and after implementation of a more liberal visitation policy. The nurses were surveyed using Stamps (1997) Index of Work Satisfaction and the Boswell Beliefs Inventory. Statistical analyses were conducted to discover nurses level of satisfaction and beliefs related to the new policy.

Results indicate that nurses were …

An Enhanced Dialogic Reading Approach To Facilitate Typically Developing Pre-School Children's Emergent Literacy Skills., Sheri E. Davis

Electronic Theses and Dissertations

This study investigated an enhanced dialogic reading (DR) approach in facilitating emergent literacy skills in typically developing preschool children. Eight children from a Title One preschool and their parents participated in five weekly 90-minute training sessions that focused on phonological awareness, print awareness, and alphabet knowledge. First order effects were examined in parent questioning and interaction behaviors on pre- and post-training videotapes. Second order effects were examined in the children's outcomes from pre- to posttesting of preliteracy, speech, and language skills. Results indicated that parents made significant increases in their initiations and responses and a significant decrease in their Mean …

Contexts For Facilitating Emergent Literacy In Typically Developing Preschoolers., Karen Nicole Wilhjelm

Electronic Theses and Dissertations

The purpose of this study was to investigate if there is an additive benefit in training Emergent Literacy (EL) skills with typically developing preschoolers using a combined intervention approach, Dialogic Reading (DR) plus classroom Phonological Awareness (CL), than a single intervention approach providing only DR training.

The study consisted of 8 preschoolers (5 DR+CL, 3 DR). The classroom PA training was conducted 3 times a week for 5 weeks for 20 minutes, using play-based activities. The DR training programs were held once a week for 5 weeks for 90 minutes teaching strategies that could be used in the home environment. …

The Type And Frequency Of Morphosyntax Errors In Children's Narratives., Jennifer Lynn Lockhart

Electronic Theses and Dissertations

A subsample of 478 children who originally participated in a larger study (Tomblin et al., 1997) was examined for type and frequency of morphological noun and verb errors in oral and written narratives in 2^nd and 4^th grade. Each child represented one of four groups: Typical Language, Specific Language Impairment, Nonspecific Language Impairment, and Low Nonverbal IQ. Three MANOVA's and post-hoc comparisons were used to test three predictions: (1) children will have more difficulty with verb than noun morphology; (2) children will make more errors in the written than the spoken narratives; and (3) children whose language impairments …

Rate Of Graduation Among Students With Histories Of Specific Language Impairment, Amy Jean Hadley

Electronic Theses and Dissertations

The rate of graduation among students with histories of specific language impairment (SLI) was investigating by comparing the target population to matched, nondisabled peers. Data regarding the graduation outcomes for 176 matched pairs of students, over a five-year period, were analyzed to determine if a significant difference in achievement of a high school diploma was observable between the two groups of students. In addition, the study addressed the variables of socioeconomic status and attendance rate as related to graduation rate. For students with histories of specific language impairment, model of service delivery was also examined with regard to graduation rate. …

Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect, Vijay Koya

Electronic Theses and Dissertations

Anthrax, a fatal bacterial infection is caused by Bacillus anthracis, a gram-positive, spore forming, capsulated, rod shaped organism. Centers for Disease Control (CDC) lists anthrax as Category A biological agent due to its severity of impact on human health, high mortality rate, acuteness of the disease and potential for delivery as a biological weapon. The currently available human vaccine in the United States (AVA anthrax vaccine adsorbed) is prepared from Alum adsorbed formalin treated supernatant culture of toxigenic, non-encapsulated strain of Bacillus anthracis with the principle component being protective antigen (PA83). Evaluation of anthrax vaccine given to nearly 400,000 US …

The Speech Situation Checklist: A Normative And Comparative Investigat, Susha Verghese

Electronic Theses and Dissertations

Studies conducted over the past decades have identified the presence of a greater amount of negative emotional reaction and speech disruption in particular speech situations among children who stutter, compared to those who do not (Brutten & Vanryckeghem, 2003b; Knudson, 1939; Meyers, 1986; Trotter, 1983). Laboratory investigations have been utilized to describe the particular situations that elicit the greatest or least amount of speech concern and fluency failures. More recently, in order to deal with the limitation of laboratory research, the use of self-report tests have gained popularity as a means of exploring the extent of negative emotional reaction and …

Effects Of Simultaneous Exercise And Speech Tasks On The Perception Of, Heather Koblick

Electronic Theses and Dissertations

The purpose of this study was to investigate the effects of voice production and perception of dyspnea in aerobic instructors during simultaneous tasks of exercise and speech production. The study aimed to document changes that occur during four conditions: 1) voice production without exercise and no use of amplification; 2) voice production without exercise and the use of amplification; 3) voice production during exercise without the use of amplification; 4) voice production during exercise with the use of amplification. Participants included ten aerobic instructors (two male and eight female). The dependent variables included vocal intensity, average fundamental frequency (F0), noise-to-harmonic …

The Role Of Erp57 In Hras Intracellular Trafficking And Function., Jaime Lyn Parman

Electronic Theses and Dissertations

Ras is a central player in signal transduction that mediates cellular proliferation and differentiation. Recent evidence has shown that lipid and non-lipid modified domains participate in Ras traffic and that plasma membrane association is mediated by vectorial vesicular transport from the endomembrane system. ERp57, an ER chaperone, has been shown to specifically bind farnesylated Hras but not non-farnesylated Hras. The objective of this study was to determine if ERp57 participates in Ras trafficking and function. First, the effect of ERp57 knock down by siRNA technology on Hras function was studied; there was a reduction in ERp57 cellular levels that led …

Flavone: The Molecular And Mechanistic Study Of How A Simple Flavonoid Protects Dna From Oxidative Damage., Jennifer Dawn Dean

Electronic Theses and Dissertations

Dietary flavonoids are ubiquitous and are marketed as supplements. Characterized as antioxidants, they offer protection against a number of degenerative diseases. Flavonoid mechanics involve free radical scavenging, metal chelation, and substrate association. The skeletal structure of flavonoids is a fused ring system modified by hydroxyl, sugar, and carbohydrate additions. Flavone is a structurally simple flavonoid. Quercetin and its glycosidic analog rutin are complex structures. Using a DNA oxidation/cleavage assay, flavone reduces DNA nicking by 91%. Depending on the solvent system used, quercetin can either increase or decrease DNA oxidation. Rutin exhibits neither pro- nor antioxidant activity. The molecular interactions responsible …

Healthcare Strategic Management: The Impact Of State And Federal Funding Levels On The Implementation Of Strategic Plans At Tennessee Hospitals., Randy Lee Byington

Electronic Theses and Dissertations

The purpose of this study was to determine hospital executive management’s perceptions of how turbulence in the politico-legal sector of the macroenvironment impacted the strategic management systems of Tennessee hospitals. In particular, how did Federal and State funding restrictions (Medicare and TennCare) impact the strategic planning and implementation process of their hospitals? The study was also designed to gain insight regarding specific changes to strategic management systems that may have resulted from these funding restrictions.

The research was conducted during April and May of 2003. Data were gathered by surveying the Chief Executive Officers (CEOs) of acute care hospitals in …

Characterization Of Sympathetic Ganglion Sensitivity To Substance P In A Genetic And A Non-Genetic Rat Model Of Hypertension., John Daniel Tompkins

Electronic Theses and Dissertations

Intravenous injection of substance P (SP) stimulates sympathetic ganglia to evoke a greater increase in renal sympathetic nerve activity, heart rate (HR) and blood pressure (BP) in hypertensive than normotensive rats due to upregulation of the NK₁ receptor. These experiments were designed to determine the cellular basis for the enhanced ganglionic responsiveness to NK₁ agonists in spontaneously hypertensive rats (SHR) in comparison to their normotensive counterparts, Wistar-Kyoto rats (WKY). Studies were also conducted to determine whether the increased ganglion responsiveness to SP in SHR is causally related to the increased BP or is a unique characteristic of this …

Phonological Awareness Training In A Preschool Classroom Of Typically Developing Children., Sara Phelps

Electronic Theses and Dissertations

The purpose of the present study was to assess the effectiveness of phonological awareness (PA) training with typically developing preschool children in a classroom setting. The PA training incorporated a range of PA skills and the training outcomes were assessed along a broad spectrum of PA abilities, pre-literacy skills, and general language abilities.

This study consisted of 21 children (11 Experimental, 10 Control). The classroom PA training program was conducted with the Experimental class in one large group for 5 weeks with 20 minute sessions conducted three times a week. A variety of fun, play-based PA activities were used with …

Protection Of Pifithrin-Α And Melatonin Against Doxorubicin-Induced Cardiotoxicity., Xuwan Liu

Electronic Theses and Dissertations

The current studies were designed to explore the protective effects of pifithrin-α and melatonin against doxorubicin-induced cardiotoxicity. Doxorubicin was injected at a dose of 22.5 mg/kg (i.p.) in mice to induce cardiotoxic effects. Meanwhile, doxorubicin caused a significant increase of cardiac cell apoptosis following injection (14.2 ± 1.1% for doxorubicin-5 d vs. 1.8 ± 0.12% for control, P < 0.01). Ribonuclease protection assays and Western blot analyses revealed that doxorubicin upregulated the p53-dependent genes Bax, BclxL, and MDM2 at least 2-fold. p53 was phosphorylated at Ser 15 in mouse hearts 1 h following doxorubicin injection, and p38 and ERK1/2 MAPKs mediated the phosphorylation of p53. In addition, caspases-3 and -9 were activated 24 h after doxorubicin injection. A p53 inhibitor, pifithrin-α, inhibited doxorubicin-induced apoptosis when administered at a dose of 2.2 mg/kg. Pifithrin-α abolished p53 transactivation activity, but did not influence doxorubicin-induced phosphorylation at Ser 15. By effectively inhibiting the expression of p53-dependent genes, pifithrin-α blocked doxorubicin-induced activation of caspases-3 and -9, thereby preventing cardiac apoptosis. In addition, pifithrin-α attenuated doxorubicin-induced structural and functional damages, without diminishing its anti-tumor efficacy on p53-null PC-3 cancer cells. The protective effects of melatonin and its metabolite 6-hydroxymelatonin on doxorubicin-induced cardiac dysfunction were evaluated in an isolated perfused mouse hearts and in vivo doxorubicin-treated mice. While perfusion of mouse hearts with 5 μM doxorubicin for 60 min resulted in a 50% suppression of HRxLVDP and a 50% reduction of coronary flow, pre-exposure of hearts to 1 μM melatonin or 6-hydroxymelatonin eased the cardiac …

Dose-Response Effects Of Lithium On Spatial Memory In The Black Molly Fish., Thomas Kyle Creson

Electronic Theses and Dissertations

Lithium continues to be widely prescribed for the management of bipolar disease, yet cognitive impairment-related side effects promote noncompliance of the treatment regimen. We have introduced a novel animal model, the black molly fish, to study dose-response effects of lithium on short-term (STM) and long-term (LTM) memories. We developed a method utilizing capillary ion analysis (CIA), to measure plasma and brain lithium levels employed in our behavioral studies. We then developed an appropriate testing environment to ascertain learning capacities of these fish. We established that black mollies could adequately perform a forced-choice spontaneous alternation (SA) task used extensively in rodents …

Activation Of Ca2+-Activated K+ Channels And Cell Migration By Hepatocyte Growth Factor/Scatter Factor In Madin-Darby Canine Kidney Cells., Min Jin

Electronic Theses and Dissertations

Hepatocyte Growth Factor/Scatter Factor (HGF/SF) stimulates migration of various cells and has been linked via Met tyrosine kinase signaling to transformation and the metastatic phenotype. HGF/SF-Met signaling also plays a role in malignancy. Migration of transformed MDCK-F cells depends on activation of a charybdotoxin (ChTX)-sensitive, volume-activated membrane K⁺ current. Patch-clamp electrophysiology and transwell migration assays were used to study the effects of HGF/SF on membrane K⁺ currents and cell migration in MDCK II cells. HGF/SF activated membrane K⁺ currents that increased over 24 hr, and these could be modulated by altering intracellular free calcium concentration [Ca^{2+ …}

Special Education Teachers' And Speech Therapists' Knowledge Of Autism Spectrum Disorder., Carol Hendrix Whaley

Electronic Theses and Dissertations

The purpose of this study was to survey special education teachers and speech therapists in eleven school districts in Northeast Tennessee regarding their knowledge level (etiology and educational programming) of autism spectrum disorder (ASD). The primary focus of the study was to identify effective programs and methods used by special educators in this region, comparing them to the latest techniques and teaching methods prescribed by recent research. In addition, identified weaknesses were used to recommend future training and staff development to enable educators to provide the best possible programs for children with autism.

Five hundred fifty-two surveys were disseminated to …

A New Immunoassay For Quantification Of A Novel Cancer Antigen In Serum And Immunostaining Of Carcinoma Tissues And Cultured Cells Revealing The Antigenic Cellular Location., Emily Christine Mcduffee

Electronic Theses and Dissertations

The purpose of this study is 1) to examine the presence of the antigen in serum by employing a newly developed ELISA immunoassay that quantifies the total antigen and bound antigen (antigen-antibody complex) using polyclonal chicken antibodies directed against an IgM-binding epitope of the new antigen, and 2) to determine the location of the antigen in carcinoma and normal cells. Sera from healthy volunteers (n = 147) and cancer patients (n = 26) were compared for both bound and total antigen concentrations using the new ELISA. Healthy volunteers were subdivided into three groups: those with a personal history of cancer …

The Signaling Pathway Of Oxysterol Induced Apoptosis In Chinese Hamster Ovary (Cho)-K1 Cells., Lin Yang

Electronic Theses and Dissertations

Apoptosis, a form of genetically programmed cell death, plays a key role in regulation of cellularity of the arterial wall. During atherogenesis, improper apoptosis may cause abnormalities of arterial morphogenesis, wall structural stability, and metabolisms. It has been well established that vascular cells undergo apoptosis after uptake of oxidized low-density lipoprotein (oxLDL). Thus, an analysis of the signaling pathway of apoptotic induction by oxLDL is of value in understanding the development of atherosclerotic plaque. In order to elucidate the signaling pathway of apoptosis induced by oxLDL, we have used Chinese hamster ovary (CHO)-K1 cells treated with a potent oxysterol, 25-hydroxycholesterol …

Hiv Tat Protein Activates Endothelial Cells Through Nfκb And Map Kinase Pathways., Jason L. Henry

Electronic Theses and Dissertations

HIV infection has been shown to predispose patients to accelerated development of heart disease. One mechanism for this pathology may involve endothelial activation either by HIV itself or by its secreted proteins, gp120 (a viral envelope protein) and tat (a protein that upregulates transcription of viral genes). We have studied the effects of gp120 and tat on signaling and production of inflammatory cytokines by Human Pulmonary Artery Endothelial Cells (HPAEC). HPAEC were stimulated at varying time points with combinations of gp120, tat, and monokines (IL-1β and TNFα). Cell lysate fractions were analyzed for MAP Kinase activity and NFκB activation, and …

30s Ribosomal Subunit Assembly Is A Target For Inhibition By Aminoglycoside Antibiotics In Escherichia Coli., Roopal Manoj Mehta

Electronic Theses and Dissertations

Antibacterial agents specific for the 50S ribosomal subunit not only inhibit translation but also prevent assembly of that subunit. I examined the 30S ribosomal subunit in growing Escherichia coli cells to see if antibiotics specific for that subunit also had a second inhibitory effect. I used the aminoglycoside antibiotics paromomycin and neomycin, which bind specifically to the 30S ribosomal subunit. Both antibiotics inhibited the growth rate, viable cell number, and protein synthesis. I used a ³H-uridine pulse and chase assay to examine the kinetics of ribosome subunit assembly in the presence and absence of each antibiotic. Analysis revealed a …

Feature Retention And Phonological Knowledge Across Children With Suspected Developmental Apraxia Of Speech, Phonological Impairment, And Typically Developing Speech., Tracy A. Ford

Electronic Theses and Dissertations

The purpose of this research effort was to examine whether the feature retention patterns and phonological knowledge of children with suspected apraxia of speech (AOSc) in comparison to those of children with phonological impairment (PI). A second purpose was to determine if a relationship exists between phonological knowledge and feature retention.

The study consisted of three groups of children: PI, AOSc, and typically developing (TD), ages four to seven. A 245-item speech sample was collected from each group. Feature retention percentages and phonological knowledge, represented by percent correct underlying representations (PCUR) were calculated for each child.

All groups retained place …

Early Academic Performance In Children With Cleft Lip And/Or Palate., Krista Leanna Lowe

Electronic Theses and Dissertations

Studies of preschool children have shown early speech and language deficits in children with cleft lip and/or palate (CLP). For some children, the deficits during kindergarten diminish as they begin school while some children continue to show delays. The purpose of this study was to determine if a relationship exists between speech and language skills and early reading skills of phonological awareness, letter identification, and rapid naming in children with and without CLP. The subjects, four kindergarten children with and four without CLP, were administered a battery of speech, language, early reading skills, and nonverbal cognition measures. Two-way analysis of …