Medicine and Health Sciences Commons^™

Does Swab Type Matter? Comparing Methods For Mannheimia Haemolytica Recovery And Upper Respiratory Microbiome Characterization In Feedlot Cattle, William B. Crosby, Lee Pinnell, John T. Richeson, Cory Wolfe, Jake Castle, John Dustin Loy, Sheryl P. Gow, Keun Seok Seo, Sarah F. Capik, Amelia R. Woolums, Paul S. Morley

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Bovine respiratory disease (BRD) is caused by interactions among host, environment, and pathogens. One standard method for antemortem pathogen identification in cattle with BRD is deep-guarded nasopharyngeal swabbing, which is challenging, costly, and waste generating. The objective was to compare the ability to recover Mannheimia haemolytica and compare microbial community structure using 29.5 inch (74.9 cm) deep-guarded nasopharyngeal swabs, 16 inch (40.6 cm) unguarded proctology swabs, or 6 inch (15.2 cm) unguarded nasal swabs when characterized using culture, real time-qPCR, and 16S rRNA gene sequencing. Samples for aerobic culture, qPCR, and 16S rRNA gene sequencing were collected from the …

Detection Of Mannheimia Haemolytica-Specific Igg, Igm And Iga In Sera And Their Relationship To Respiratory Disease In Cattle, Korakrit Poonsuk, Carita Kordik, Matthew M. Hille, Ting-Yu Cheng, William B. Crosby, Amelia R. Woolums, Michael L. Clawson, Carol G. Chitko-Mckown, Bruce Brodersen, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test …

First Gene-Edited Calf With Reduced Susceptibility To A Major Viral Pathogen, Aspen M. Workman, Michael P. Heaton, Brian L. Vander Ley, Dennis A. Webster, Luke Sherry, Jonathan R. Bostrom, Sabrina Larson, Theodore S. Kalbfleisch, Gregory P. Harhay, Erin E. Jobman, Daniel F. Carlson, Tad S. Sonstegard

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine viral diarrhea virus (BVDV) is one of the most important viruses affecting the health and well-being of bovine species throughout the world. Here, we used CRISPR-mediated homology-directed repair and somatic cell nuclear transfer to produce a live calf with a six amino acid substitution in the BVDV binding domain of bovine CD46. The result was a gene-edited calf with dramatically reduced susceptibility to infection as measured by reduced clinical signs and the lack of viral infection in white blood cells. The edited calf has no off-target edits and appears normal and healthy at 20 months of age without obvious …

Aerobic Exercise And Scaffolds With Hierarchical Porosity Synergistically Promote Functional Recovery Post Volumetric Muscle Loss, Christina Zhu, Karina Skylar, Yori Endo, Mohamadmahdi Samandari,, Mehran Karvar, Azadeh Mostafavi, Jacob Quint, Chiara Rioldi, Wojciech Swieszkowski, Joshua Mauney, Shailesh Agarwal, Ali Tamayol, Indranil Sinha

School of Veterinary and Biomedical Sciences: Faculty Publications

Volumetric muscle loss (VML) is a composite loss of skeletal muscle tissue (greater than 20%) that heals with minimal muscle regeneration, substantial fibrosis, and subsequent functional deficits. Standard treatment, involving free functional muscle transfer and physical therapy, cannot restore full muscle function following VML. Tissue engineered scaffolds, 3D structural templates that mimic native extracellular matrix, are promising to enhance functional muscle formation and recovery. Bioprinted 3D scaffolds are engineered using bioinks, created from scaffolding material, cells, and growth factors, to replicate skeletal muscle architecture with precise control over their spatial deposition. METHODS: The present study evaluates a 3D-printed foam-like scaffold …

Host-Genetic-Based Outcome Of Co-Infection By Pcv2b And Prrsv In Pigs, Christian W. Eaton, Hiep Vu, Arabella L. Hodges, Seth P. Harris, Stephen D. Kachman, Daniel C. Ciobanu

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication of porcine circovirus type 2 (PCV2), an important worldwide swine pathogen, has been demonstrated to be influenced by host genotype. Specifically, a missense DNA polymorphism (SYNGR2 p.Arg63Cys) within the SYNGR2 gene was demonstrated to contribute to variation in PCV2b viral load and subsequent immune response following infection. PCV2 is known to induce immunosuppression leading to an increase in susceptibility to subsequent infections with other viral pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV). In order to assess the role of SYNGR2 p.Arg63Cys in co-infections, pigs homozygous for the favorable SYNGR2 p.63Cys (N = 30) …

Infection Of Feral Phenotype Swine With Japanese Encephalitis Virus, So Lee Park, Yan-Jang S. Huang, Amy C. Lyons, Victoria B. Ayers, Susan M. Hettenbach, D. Scott Mcvey, Leela E. Noronha, Kenneth R. Burton, Stephen Higgs, Dana L. Vanlandingham

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic flavivirus and the leading cause of pediatric encephalitis in the Asian Pacific region. The transmission cycle primarily involves Culex spp. mosquitoes and Ardeid birds, with domestic pigs (Sus scrofa domestica) being the source of infectious viruses for the spillover of JEV from the natural endemic transmission cycle into the human population. Although many studies have concluded that domestic pigs play an important role in the transmission cycle of JEV, and infection of humans, the role of feral pigs in the transmission of JEV remains unclear. Since domestic and …

Diagnosing Neurocysticercosis In Skeletonized Human Remains Of Forensic Importance, John O. Obafunwa, Karl Reinhard

Karl Reinhard Publications

Neurocysticercosis is endemic in many parts of the underdeveloped and developing countries, with continuous presence in developed countries due to the influx of migrants from regions where the diseases are endemic. Neuroimaging, anatomic pathological techniques, immunodiagnostic tests, clinical examination and epidemiologic considerations will easily provide the diagnosis. However, physicians in developed countries are perhaps progressively missing the diagnosis, and need to re-acquaint themselves with the condition and acquire a high suspicion index. The authors present a medicolegal case where the forensic team made a conclusion of neurocysticercosis (among other diagnoses), following post mortem examination of a largely skeletonized and mummified …

Exploring The Evolution Of Trypanosoma Cruzi And The Emergence Of Chagas Disease In The Context Of Environmental Change: Applying The Stockholm Paradigm To Archaeoparasitology, Part I, Aida Romera Barbera, Daniel R. Brooks, T. Michael Fink, Karl J. Reinhard

Karl Reinhard Publications

The Stockholm Paradigm is an evolutionary synthesis that explains the emergence of novel pathogens in the context of environmental disturbances. Considering the urgent climate change situation, anticipation stands as key to prevent and mitigate the effects that climate change can have in the emergence of pathogens. However, the success of preventive measures is hindered by a limited knowledge of the interplaying dynamics between biology, environment, and culture leading to emerging infectious diseases. For many decades, bioarchaeologists have been gathering data on past human-environment interactions, therefore contributing to the conversation. Archaeoparasitology, at the intersection between pathology, bioarchaeology, and biology, is uniquely …

Documenting The Presence Of Chagas Disease In Prehistoric North America: Applying The Stockholm Paradigm To Archaeoparasitology, Part Ii, Karl J. Reinhard, Daniel R. Brooks, Aida Romera Barbera, T. Michael Fink

Karl Reinhard Publications

The DAMA protocol (Document, Assess, Monitor, Act) is the applied aspect of the Stockholm Paradigm. Evidence from archaeology and history of medicine has a demonstrated value in documenting infection risk for specific study areas. Bioarchaeological remains and historical documents show vivid examples of pathology related to the Chagas disease life cycle. The science of archaeology defines the lifestyles that created risk of triatomine kissing bug infestations and trypanosome protozoa infections. Paleogenetics, in the form of molecular analyses of Trypanosoma cruzi, elucidate the temporal origins of genetic variants and adaptation to human environments. We illustrate how these fields reveal the risk …

Revisiting The Rainbow: Culturally Responsive Updates To A Standard Clinical Resource, Angela M. Dietsch, Richard Mocarski, Debra A. Hope, Nathan Woodruff, Miechelle Mckelvey

Department of Special Education and Communication Disorders: Faculty Publications

Purpose

Cultural responsivity is essential for efficacious and affirming clinical relationships. This may be especially important with historically marginalized clients, such as transgender and gender-diverse (TGD) people seeking behaviorally based affirming communication services. We recommend modifications to standard tools for diagnostics and training that otherwise might undermine our efforts to create an inclusive and affirming environment.

Method

Modifications to the Rainbow Passage, a standardized paragraph utilized for eliciting speech samples in clinical settings, focused on nongendered terminology and the elimination of content with religious connotations.

Results

The recommended edits to the Rainbow Passage maintain similar length, cadence, and phonetic balance …

Brain Activity Associated With Taste Stimulation: A Mechanism For Neuroplastic Change?, Angela M. Dietsch, Ross M. Westemeyer, Douglas H. Schultz

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: Neuroplasticity may be enhanced by increasing brain activation and bloodflow in neural regions relevant to the target behavior.We administered precisely formulated and dosed taste stimuli to determine whether the associated brain activity patterns included areas that underlie swallowing control.

Methods: Five taste stimuli (unflavored, sour, sweet-sour, lemon, and orange suspensions) were administered in timing-regulated and temperature-controlled 3 mL doses via a customized pump/tubing system to 21 healthy adults during functional magnetic resonance imaging (fMRI). Whole-brain analyses of fMRI data assessed main effects of taste stimulation as well as differential effects of taste profile.

Results: Differences in …

Range-Wide Sources Of Variation In Reproductive Rates Of Northern Spotted Owls, Jeremy T. Rockweit, Julianna M. Jenkins, James E. Hines, James D. Nichols, Katie M. Dugger, Alan B. Franklin, Peter C. Carlson, William L. Kendall, Damon B. Lesmeister, Christopher Mccafferty, Steven H. Ackers, L. Steven Andrews, Larissa L. Bailey, Jesse Burgher, Kenneth P. Burnham, Tara Chestnut, Mary M. Conner, Raymond J. Davis, Krista E. Dilione, Eric D. Forsman, Elizabeth M. Glenn, Scott A. Gremel, Keith A. Hamm, Dale R. Herter, J. Mark Higley, Rob B. Horn, David W. Lamphear, Trent L. Mcdonald, Janice A. Reid, Carl J. Schwarz, David C. Simon, Stan G. Sovern, James K. Swingle, J. David Wiens, Heather Wise, Charles B. Yackulic

United States Department of Agriculture Wildlife Services: Staff Publications

We conducted a range-wide investigation of the dynamics of site-level reproductive rate of northern spotted owls using survey data from 11 study areas across the subspecies geographic range collected during 1993–2018. Our analytical approach accounted for imperfect detection of owl pairs and misclassification of successful reproduction (i.e., at least one young fledged) and contributed further insights into northern spotted owl population ecology and dynamics. Both nondetection and state misclassification were important, especially because factors affecting these sources of error also affected focal ecological parameters. Annual probabilities of site occupancy were greatest at sites with successful reproduction in the previous year …

Demographic And Functional Responses Of Kit Foxes To Changes In Prey Abundance, Ashley E. Hodge, Eric M. Gese, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Many carnivores exhibit demographic and functional responses to changes in prey abundance. Demographic responses often include changes in population size, litter size, and recruitment of young into the adult population. Functional feeding responses are commonly reported for many carnivore species. We investigated demographic and functional responses of kit foxes (Vulpes macrotis Merriam, 1888) to changes in prey abundance during 2010–2013 in western Utah, USA. Between 2010 and 2013, litter size averaged 3.9 (±1.4) pups/litter. Survival rates of kit fox pups were 0.07, 0.01, 0.46, and 0.16, respectively, and there was a correlation between pup survival rates and rodent abundance; …

Comparison Of An Antioxidant Source And Antioxidant Plus Bcaa On Athletic Performance And Post Exercise Recovery Of Horses, Emily Kent, Stephen J. Coloman, Jason E. Bruemmer, Renan Regatieri Casagrande, Christine Levihn, Grace Romo, Kevin Herkelman, Tanja Hess

United States Department of Agriculture Wildlife Services: Staff Publications

Antioxidant supplementation decreases postexercise oxidative stress but could also decrease muscle pro- tein synthesis. This study compared the effects of three diets: low antioxidant (control, CON), high an- tioxidant (AO), and branched-chain amino acid high antioxidant (BCAO) supplementation on postexercise protein synthesis and oxidative stress. We hypothesized that supplementing antioxidants with branched- chain amino acids(BCAA) would reduce oxidative stress without hindering muscle protein synthesis. Eigh- teen mixed-breed polo horses (11 mares and 7 geldings, with age range between 5 and 18 years, were on CON diet for 30 days (from day -45 until day 0) and then were assigned to …

High Winds And Melting Sea Ice Trigger Landward Movement In A Polar Bear Population Of Concern, Annie Kellner, Todd C. Atwood, David C. Douglas, Stewart W. Breck, Colorado State University - Fort Collins

United States Department of Agriculture Wildlife Services: Staff Publications

Some animal species are responding to climate change by altering the timing of events like mating and migration. Such behavioral plasticity can be adaptive, but it is not always. Polar bears (Ursus maritimus) from the southern Beaufort Sea subpopulation have mostly remained on ice year-round, but as the climate warms and summer sea ice declines, a growing proportion of the subpopulation is summering ashore. The triggers of this novel behavior are not well understood. Our study uses a parametric time-to-event model to test whether biological and/or time-varying environmental variables thought to influence polar bear movement and habitat selection …

Space Use And Movements Of Southeastern Breeding Double-Crested Cormorants (Nannopterum Auritum) In The United States, Leah L.K. Moran, Brian S. Dorr, Katie C. Hanson-Dorr, R. J. Moore, Scott A. Rush

United States Department of Agriculture Wildlife Services: Staff Publications

Abstract: Seasonal movements of Double-crested Cormorants (Nannopterum auritum) have been studied at breeding and wintering sites in the southeastern United States, but little information exists on the movements of these birds within and from their southern breeding sites in lacustrine systems. Since 2001, cormorants have established nesting colonies on islands in Guntersville Reservoir in Alabama, USA. Following the movements of tagged cormorants using satellite telemetry, we found that the mean home range during the 2017 breeding season (May–August) was 41.76 km², with a core use area of 6.36 km². The mean home range used …

Behavior Of Wild Pigs Toward Conspecific Carcasses: Implications For Disease Transmission In A Hot, Semiarid Climate, Samantha Leivers, Tyler Campbell, Michael J. Bodenchuk, John Tomeĉek

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) are a prolific, invasive species in the United States of America and act as vectors for many pathogens. An emerging pathogen of concern to the USA is African swine fever (ASF), a deadly viral disease affecting swine that is endemic to Africa and has spread to parts of Europe, Asia, and the Caribbean. ASF affects both wild and domesticated pigs and can be transmitted via several avenues, including interactions between and consumption of dead pigs by their live conspecifics. As wild pigs are considered a serious threat in the transmission of ASF, understanding the …

H7n9 Influenza A Virus Transmission In A Multispecies Barnyard Model, Angela M. Bosco-Lauth, Anna Rodriguez, Rachel M. Maison, Stephanie M. Porter, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

Influenza A viruses are a diverse group of pathogens that have been responsible for millions of human and avian deaths throughout history. Here, we illustrate the transmission potential of H7N9 influenza A virus between Coturnix quail (Coturnix sp.), domestic ducks (Anas platyrhynchos domesticus), chickens (Gallus gallus domesticus), and house sparrows (Passer domesticus) co-housed in an artificial barnyard setting. In each of four replicates, individuals from a single species were infected with the virus. Quail shed virus orally and were a source of infection for both chickens and ducks. Infected chickens transmitted the virus to quail but not to ducks or …

Genomic Analyses Of Gray Fox Lineages Suggest Ancient Divergence And Secondary Contact In The Southern Great Plains, Elizabeth M. Kierepka, Sophie Preckler-Quisquater, Dawn M. Reding, Antoinette Piaggio, Seth P.D. Riley, Benjamin N. Sacks

United States Department of Agriculture Wildlife Services: Staff Publications

The gray fox (Urocyon cinereoargenteus) lineage diverged from all other extant canids at their most basal node and is restricted to the Americas. Previous mitochondrial analysis from coastal populations identified deeply divergent (up to 1 Mya) eastern and western lineages that predate most intraspecific splits in carnivores. We conducted genotyping by sequencing and mitochondrial analysis on gray foxes sampled across North America to determine geographic concordance between nuclear and mitochondrial contact zones and divergence times. We also estimated the admixture within the contact zone between eastern and western gray foxes based on nuclear DNA. Both datasets confirmed that …

Writing Statistical Methods For Ecologists, Amy J. Davis, Shannon Kay

United States Department of Agriculture Wildlife Services: Staff Publications

The Methods section is a key component of any ecology research publication containing detailed information on how the data were collected and analyzed. However, descriptions of which statistical methods were used and how they were applied can substantially vary and may not provide enough information for the analyses to be reproducible. Computational and statistical programming advances have allowed ecological researchers without a strong statistical or mathematical background to access and use increasingly complex statistical methods. Thus, statistical methods are written by and need to be accessible to researchers across a range of quantitative expertise. Poorly written Methods sections can incorrectly …

Variance In Offspring Sex Ratio And Maternal Allocation In A Highly Invasive Mammal, Sarah M. Chinn, Timothy J. Smyser, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Skewed sex ratios at birth are widely reported in wild populations, however, the extent to which parents are able to modulate the sex ratio of offspring to maximize their own fitness remains unclear. This is particularly true for highly polytocous species as maximizing fitness may include trade-offs between sex ratio and the size and number of offspring in litters. In such cases, it may be adaptive for mothers to adjust both the number of offspring per litter and offspring sex to maximize individual fitness. Investigating maternal sex allocation in wild pigs (Sus scrofa) under stochastic environmental conditions, we predicted that …

The Past, Present, And Future Of Equine Science, Sarah H. White-Springer, Jason E. Bruemmer, Robert J. Coleman

United States Department of Agriculture Wildlife Services: Staff Publications

A core group of 27 equine nutritionists and physiologists joined together in the late 1960s to formally address and enhance the direction of equine research, creating the Equine Nutrition and Physiology Soci- ety. In 2003, that growing society transformed into the Equine Science Society, which now serves as the preeminent, internationally recognized scientific equine organization. In recent years, it has been appre- ciated that equine science encompasses a wide range of focus areas, including exercise science, nutrition, genetics, reproductive physiology, teaching and extension, production and management, and mix of other specialties, qualified as biosciences. Additionally, trainees are highly valued in …

Invasive Frogs Show Persistent Physiological Differences To Elevation And Acclimate To Colder Temperatures, Jack R. Marchetti, Karen H. Beard, Emily E. Virgin, Erin L. Lewis, Steven C. Hess, Kwanho C. Ki, Layne O. Sermersheim, Adriana P. Furtado, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

The coqui frog (Eleutherodactylus coqui) was introduced to the island of Hawai’i in the 1980s and has spread across much of the island. Concern remains that this frog will continue to expand its range and invade higher elevation habitats where much of the island’s endemic species are found. We determined whether coqui thermal tolerance and physiology change along Hawai’i’s elevational gradients. We measured physiological responses using a short-term experiment to determine baseline tolerance and physiology by elevation, and a long-term experiment to determine the coqui’s ability to acclimate to different temperatures. We collected frogs from low, medium, and …

Integrating Robotics Into Wildlife Conservation: Testing Improvements To Predator Deterrents Through Movement, Stewart W. Breck, Jeffrey T. Schultz, David Prause, Cameron Krebs, Anthony J. Giordano, Byron Boots

United States Department of Agriculture Wildlife Services: Staff Publications

Background Agricultural and pastoral landscapes can provide important habitat for wildlife conservation, but sharing these landscapes with wildlife can create conflict that is costly and requires managing. Livestock predation is a good example of the challenges involving coexistence with wildlife across shared landscapes. Integrating new technology into agricultural practices could help minimize human-wildlife conflict. In this study, we used concepts from the fields of robotics (i.e., automated movement and adaptiveness) and agricultural practices (i.e., managing livestock risk to predation) to explore how integration of these concepts could aid the development of more effective predator deterrents.

Methods …

Transmission Of Sars-Cov-2 In Free-Ranging White-Tailed Deer In The United States, Aijing Feng, Sarah Bevins, Jeff Chandler, Thomas J. Deliberto, Ria Ghai, Kristina Lantz, Julianna Lenoch, Adam Retchless, Susan A. Shriner, Cynthia Y. Tang, Suxiang Sue Tong, Mia Torchetti, Anna Uehara, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

SARS-CoV-2 is a zoonotic virus with documented bi-directional transmission between people and animals. Transmission of SARS-CoV-2 from humans to free-ranging white-tailed deer (Odocoileus virginianus) poses a unique public health risk due to the potential for reservoir establishment where variantsmay persist and evolve. We collected 8,830 respiratory samples from free-ranging white-tailed deer across Washington, D.C. and 26 states in the United States between November 2021 and April 2022. We obtained 391 sequences and identified 34 Pango lineages including the Alpha, Gamma, Delta, and Omicron variants. Evolutionary analyses showed these white-tailed deer viruses originated fromat least 109 independent spillovers fromhumans,which resulted in …

A Social Network Analysis Of Actors Involved In Wild Pig (Sus Scrofa) Management In Missouri, Hailey E. Ellis, Meredith J. Grady, Lauren Jaebker, Alan D. Bright, Keith M. Carlisle

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) cause significant damage to agriculture and native ecosystems and can transmit diseases to animals and people. Management responses designed to reduce population numbers are needed to mitigate these threats. Identifying networks of key actors, including the ways in which they interact, is valuable for purposes of better understanding opportunities or constraints that generate or impede effective management responses. The goal of our study was to understand the network of organizations, and the personnel working within them, that were active in wild pig management, research, or policy initiatives in Missouri during 2018–2020 by 1) identifying …

Assessment Of Habitat‐Specific Competition For Oral Rabies Vaccine Baits Between Raccoons And Opossums, James L. Helton, Jacob E. Hill, David A. Bernasconi, Wesley C. Dixon, Richard B. Chipman, Amy Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

Throughout the eastern United States, the National Rabies Management Program (NRMP) distributes oral rabies vaccine (ORV) baits to manage rabies virus circulation in raccoon (Procyon lotor) populations. The consumption of vaccine baits by non‐target species including Virginia opossums (Didelphis virginiana) may reduce the effectiveness of ORV programs, but competition for baits remains poorly quantified in many areas of the southeastern United States. We distributed placebo ORV baits injected with a biomarker across 4 land cover types (bottomland hardwood, upland pine, riparian, isolated wetland) on the Savannah River Site in South Carolina, USA, 2017–2019. We then trapped …

Fusion Of Visible And Thermal Images Improves Automated Detection And Classification Of Animals For Drone Surveys, B. Santhana Krishnan, Landon R. Jones, Jared A. Elmore, Sathishkumar Samiappan, Kristine O. Evans, Morgan Pfeiffer, Bradley Blackwell, Raymond Iglay

United States Department of Agriculture Wildlife Services: Staff Publications

Visible and thermal images acquired from drones (unoccupied aircraft systems) have substantially improved animal monitoring. Combining complementary information from both image types provides a powerful approach for automating detection and classification of multiple animal species to augment drone surveys. We compared eight image fusion methods using thermal and visible drone images combined with two supervised deep learning models, to evaluate the detection and classification of white-tailed deer (Odocoileus virginianus), domestic cow (Bos taurus), and domestic horse (Equus caballus). We classified visible and thermal images separately and compared them with the results of image fusion. …

Spatial Ecology Of Translocated Raccoons, Jacob E. Hill, James L. Helton, Richard B. Chipman, Amy Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes

United States Department of Agriculture Wildlife Services: Staff Publications

Raccoons (Procyon lotor) are routinely translocated both legally and illegally to mitigate conflicts with humans, which has contributed to the spread of rabies virus across eastern North America. The movement behavior of translocated raccoons has important ramifications for disease transmission yet remains understudied and poorly quantified. To examine the spatial ecology of raccoons following experimental translocation, we performed reciprocal 16 km-distance translocations of 30 raccoons between habitats of high and low raccoon density (bottomland hardwood and upland pine, respectively) across the Savannah River Site (SRS) in Aiken, South Carolina, USA (2018–2019). Translocation influenced patterns of raccoon space use, …

Demographic, Environmental And Physiological Predictors Of Gastrointestinal Parasites In Urban Raccoons, Liana F. Wait, Shylo R. Johnson, Kathleen M. Nelson, Richard B. Chipman, Frederick E. Pogmore, Andrew P. Dobson, Andrea L. Graham

United States Department of Agriculture Wildlife Services: Staff Publications

Raccoons are host to diverse gastrointestinal parasites, but little is known about the ecology of these parasites in terms of their interactions with each other during coinfections, their interactions with host physiology and environmental factors, and their impact on raccoon health and survival. As a first step, we investigated the patterns of parasite infection and their demographic distribution in an urban-suburban population of raccoons trapped in the summers and autumns of 2018 and 2019. We collected faecal samples, demographic data, morphometric measurements, and blood smears, and used GPS data to classify trapping location by land cover type. Faecal floats were …