Medicine and Health Sciences Commons^™

A Study Of Euplotes Crassus Telomere Proteins And Related Genes, John Scott Perez

Department of Chemistry: Dissertations, Theses, and Student Research

A novel PCR technique used to amplify Euplotes crassus macronuclear chromosomes was developed to provide scientific proof that an Euplotes crassus 1.0 kb gene is not genetically related to an Oxytricha nova 1.8 kb β-telomere protein gene. Theories and experimental procedures associated with the development of this PCR technique were the product of research that will have a profound impact on future studies of telomere protein genes and telomeric DNA in a multitude of eukaryotic cells. The hypothesis that a β-telomere protein does not exist in Euplotes crassus was supported by the result of this study.

Expression of the recombinant …

Factors Affecting Rats' Location During Conditioned Suppression Training, Rick A. Bevins, John J. B. Ayres

Department of Psychology: Faculty Publications

If freezing underlies barpress conditioned suppression, then it seems odd that auditory cues paired with shock evoke more freezing than do visual cues, yet evoke similar suppression. Bevins and Ayres (1992) found that auditory and visual cues also evoked similar withdrawal from the bar and dipper areas and suggested that such withdrawal could explain the similar suppression. Seeking to understand that withdrawal, we found evidence in the present study that it was due either to adventitious punishment or to place-aversion learning. The cue for shock seemed to set the occasion for such learning. For example, we found that, as training …

An Abattoir Study Of Tuberculosis In A Herd Of Farmed Elk, Terry L. Whiting, Stacy V. Tessaro

Michigan Bovine Tuberculosis Bibliography and Database

The purpose of this study was to examine the prevalence and distribution of grossly visible lesions of tuberculosis in a herd of 344 North American elk (Cervus elaphus) depopulated during a three-month period in 1991. Abattoir inspection detected mycobacterial lesions in 134 (39.8%) of the 337 animals received for slaughter. The prevalence of lesions increased with the age of the animals. Lesions were predominantly suppurative rather than caseous, and mineralization was less evident than in tuberculous lesions in cattle. Lesions occurred predominantly -in lymph nodes, and lungs were the only organs in which mycobacterial lesions were found. The …

Trends And Differentials In Mortality From Cancers Of The Oral Cavity And Pharynx In The United States, 1973-1 987, Howard I. Goldberg, Stuart A. Lockwood, Stephen W. Wyatt, Linda S. Crossett

United States Public Health Resources

Background. This analysis consisted of an examination of trends and differentials in mortality from cancers of the oral cavity and pharynx in the United States for a recent 15-year period.

Methods. The authors have used national cause of death data for the United States and intercensal population estimates to examine mortality from oral and pharyngeal cancers between 1973 and 1987 and to study differentials according to gender, race, and region of residence.

Results. The overall mortality rate from these cancers decreased by 19% during the 15-year period, with most of the decline occurring after 1979. Mortality was …

Effecting Change By The Use Of Consultative Coaching With The Primary Caregivers Of Language Delayed Preschoolers, Sari Jeanne Giles

Open Access Master's Theses (through 2010)

This study examined the effect of a speech-language pathologist using consultative coaching with the primary caregivers (PCG) of two expressive language delayed preschool children. The coaching was designed to improve the PCG’s use of specific communication behaviors and strategies to facilitate growth in the child’s communicative behaviors. Specifically, the consultative coaching encouraged the PCG to (1) follow the child’s lead (2) use self and parallel talk (3) expand on the child’s verbal productions and (4) use open-ended questions. Two dyads, made up of expressive language delayed preschool children and their primary caregivers, were the subjects in single subject multiple-baseline designs. …

Social Networks And Daily Activities Of Street Youth In Belo Horizonte, Brazil, Regina Campos, Marcela Raffaelli, Walter Ude, Marilia Greco, Andrea Ruff, Jon Rolf, Carlos Mauricio Antunes, Neal Halsey, Dirceu Greco, Mauro Jeronymo, Carl Kendall, Alice Payne Merritt, Jorge Andrade Pinto, Eliana Siqueira, Barbara De Zalduondo

Department of Psychology: Faculty Publications

Social networks and daily activities of children and adolescents living and/or working on the streets of a large Brazilian city were examined. Drawing on data collected through structured surveys, in-depth interviews, focus group discussions, and field observations, we described street youths’ family situation, social resources, institutional experiences, survival activities, and problem behaviors and investigated differences attributable to age, gender, and living situation (at home or on the street). Youngsters who lived at home and worked on the street appeared to be experiencing orderly development despite their impoverished circumstances. Youngsters who lived on the street showed hallmarks of psychological and physical …

The Identification And Reporting Of Physical Abuse By Physicians: A Review And Implications For Research, Jody E. Warner, David J. Hansen

Department of Psychology: Faculty Publications

Identification and reporting of possible cases of child physical abuse are critical precursors to intervention with maltreating families. Professionals from a variety of disciplines are mandated to report suspected cases of child maltreatment. Unfortunately, not all physically abused children are identified or reported. This paper reviews the literature that has examined factors that may influence the identification and reporting of physical abuse by physicians. The literature review is preceded by an overview of the multistep, multibehavior process of identification and reporting. The factors that may influence identification and reporting are discussed according to their association with the case, physician, or …

Extra-Legal Factors And Product Liability: The Influence Of Mock Jurors’ Demographic Characteristics And Intuitions About The Cause Of An Injury, Brian H. Bornstein, Michelle Rajki

Department of Psychology: Faculty Publications

Two experiments were performed to investigate the role of extra-legal factors in a simulated product liability trial. In cases where the factual evidence was identical, subjects’ liability judgments varied as a function of the case-specific factor of the alleged source of the plaintiff’s injury. In deciding cases differently depending on the alleged cause, subjects relied on intuitions about what injury sources are more or less likely to cause a certain kind of injury. Juror- specific factors also influenced subjects’ verdicts. There was no difference between students and non-students, but race and SES—factors that are often correlated with student status—did affect …

Identification And Characterization Of A Human Herpesvirus 6 Gene Segment Capable Of Transactivating The Human Immunodeficiency Virus Type 1 Long Terminal Repeat In An Spl Binding Site-Dependent Manner, Jinhai Wang, Clinton J. Jones, Michael Norcross, Ernst Bohnlein, Abdur Razzaque

School of Veterinary and Biomedical Sciences: Faculty Publications

The human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) is transactivated by various extracellular signals and viral cofactors that include human herpesviruses. These transactivators are capable of transactivating the HIV-1 LTR through the transactivation response element, NF-KB, or other regulatory binding elements. Human herpesvirus 6 (HHV-6) is a potential cofactor of HIV-1. Here, we report that an HHV-6 gene segment, ZVH14, which can neoplastically transform NIH 3T3 and human keratinocytes, is capable of transactivating HIV-1 LTR chloramphenicol acetyltransferase constructs in an Spl binding site-dependent manner. Transactivation increased synergistically in the presence of multiple Spl sites and was dramatically …

Wild Medicine: Review Of Medicinal Wild Plants Of The Prairie By Kelly Kindscher, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

I am highly enthusiastic about this book as a reference work. It summarizes the literature on the medicinal uses for more than 103 prairie plants. For 43 species, Kindscher gives common, Indian, and scientific names; description; habitat; parts used; Indian uses; medical history; scientific research and cultivation; and information on about 100 of their relatives. Another 60 entries are condensed to 24 paragraphs. Full-page line drawings by William S. Whitney of the major species are generally excellent, and all 103 entries have distribution maps. The introduction discusses the region covered, with helpful maps. There's an index, a glossary and a …

Socialization Of Children’S Vicarious Emotional Responding And Prosocial Behavior: Relations With Mothers' Perceptions Of Children's Emotional Reactivity , Richard A. Fabes, Nancy Eisenberg, Mariss Karbon, Jane Bernzweig, Anna Lee Speer, Gustavo Carlo

Department of Psychology: Faculty Publications

We examined mother-child emotion-related interactions and how these interactions related to mothers’ perceptions of children’s emotional reactivity. Mothers of 49 kindergartners and 54 2nd graders told their children 2 stories about distressed others. Children’s emotional, physiological, and prosocial responses were also obtained. Mothers rated children’s tendencies to become emotional when exposed to distressed others. For kindergartners, mothers’ perceptions of children’s emotional reactivity were positively related to her use of positive facial expressions. Mothers’ perceptions of 2nd graders’ emotional reactivity were inversely related to maternal responsiveness. For both age groups, children’s skin conductance was inversely related to helpfulness. These findings suggest …

A Multiplicative Model Of The Dispositional Antecedents Of A Prosocial Behavior: Predicting More Of The People More Of The Time, George Knight, Lora G. Johnson, Gustavo Carlo, Nancy Eisenberg

Department of Psychology: Faculty Publications

Researchers have demonstrated that individual differences in prosocial behavior may be a function of dispositional or person variables. However, the observed empirical relations have been relatively modest, perhaps because researchers have most often examined simple additive or single predictor models. The present investigation examined a multiplicative model of the relation of dispositional variables to a prosocial behavior. Eighty-six children between 6 and 9 years old completed a monetary donation task and measures of the general tendency to understand and reason about the affective state of others, to be sympathetic, and to understand the units and value of money. As expected, …

Serologic And Genetic Identification Of Peromyscus Maniculatus As The Primary Rodent Reservoir For A New Hantavirus In The Southwestern United States, James E. Childs, Thomas G. Ksiazek, Christina F. Spiropoulou, John W. Krebs, Sergey Morzunov, Gary O. Maupin, Kenneth L. Gage, Pierre E. Rollin, John Sarisky, Russell E. Enscore, Jennifer K. Frey, C. J. Peters, Stuart T. Nichol

Zoonotics and Wildlife Disease: Publications

An outbreak of hantavirus pulmonary syndrome(H PS) in the southwestern United States was etiologically linked to a newly recognized hantavirus. Knowledge that hantaviruses are maintained in rodent reservoirs stimulated a field and laboratory investigation of 1696 small mammals of 31 species. The most commonly captured rodent, the deer mouse( Peromyscus maniculatus), had the highest antibody prevalence(3 %)to four hantavirus antigens. Antibody also was detected in 10 other species of rodent and in 1 species of rabbit. Reverse transcriptase-polymerase chain reaction( RT-PCR)products of hantavirus from rodent tissues were indistinguishable from those from human H PS patients. More than 96% of …

White-Tailed Deer As A Potential Reseroir Of Ehrlichia Spp., Jacqueline E. Dawson, James E. Childs, Kristine L. Biggie, Charla Moore, David Stalknecht, John Shaddock, John Bouseman, Erik Hofmeister, James G. Olson

Zoonotics and Wildlife Disease: Publications

We determined the antibody prevalence to Ehrlichia spp., in white-tailed deer (Odocoileus virginianus) and the geographic distribution of seropositive animals in 84 counties in Alabama, Arkansas, Florida, Georgia, Illinois, Kentucky, Louisiana, Maryland, Massachusetts, Mississippi, Missouri, North Carolina, South Carolina, Tennessee, Texas, Virginia, and West Virginia (USA). Using an indirect fluorescent antibody test we detected antibodies (≥ 1:128) to this bacterium in 544 (43%) of 1269 deer. Presence of antibodies to Ehrlichia spp. was related to a southerly latitude, low elevation, and resulting milder climatic conditions. It appears that whitetailed deer were naturally infected with Ehrlichia spp.; the infection …

Expression Of Functional Protease And Subviral Particles By Vaccinia Virus Containing Equine Infectious Anaemia Virus Gag And 5' Pol Genes, Travis C. Mcguire, Katherine I. O'Rourke, Timothy V. Baszler, Steven R. Leib, Alberta L. Brassfield, William C. Davis

Zoonotics and Wildlife Disease: Publications

Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.

Scid Mouse Spleen Does Not Support Scrapie Agent Replication, Katherine I. O'Rourke, T. P. Huff, C. W. Leathers, M. M. Robinson, J. R. Gorham

Zoonotics and Wildlife Disease: Publications

BALB/c and severe combined immunodeficiency (SCID) mice were inoculated intracerebrally or intraperitoneally with scrapie agent strain ME7 to examine the role of functional lymphocytes and follicular dendritic cells in splenic infectivity and PrP^sc accumulation. Intracerebrally inoculated BALB/c and SCID mice developed the clinical signs and microscopic lesions characteristic of scrapie. Spleens from terminally affected BALB/c mice contained PrP^sc which was detectable by immunoblot analysis; SCID mouse spleens did not contain detectable PrP so. SCID mouse spleens collected during the first 90 days after intraperitoneal infection contained neither infectivity nor PrP^sc.

Major Histocompatibility Complex-Restricted Cd8+ Cytotoxic T Lymphocytes From Horses With Equine Infectious Anemia Virus Recognize Env And Gag/Pr Proteins, Travis C. Mcguire, Daniel B. Tumas, Katherine M. Byrne, Melissa T. Hines, Steven R. Leib, Alberta L. Brassfield, Katherine I. O'Rourke, Lance E. Perryman

Zoonotics and Wildlife Disease: Publications

Cytotoxic T lymphocytes (CTL) can control some viral infections and may be important in the control of lentiviruses, including human immunodeficiency virus type 1. Since there is limited evidence for an in vivo role of CTL in control of lentiviruses, dissection of immune mechanisms in animal lentiviral infections may provide needed information. Horses infected with equine infectious anemia virus (EIAV), a lentivirus, have acute plasma viremia which is terminated in immunocompetent horses. Viremic episodes may recur, but most horses ultimately control infection and become asymptomatic carriers. To begin dissection of the immune mechanisms involved in EIAV control, peripheral blood mononuclear …

A Novel Protein, Csg2p, Is Required For Ca2+ Regulation In Saccharomyces Cerevisiae, Troy Beeler, Kenneth Gable, Chun Zhao, Teresa Dunn

Uniformed Services University of the Health Sciences

Nineteen mutants that lost the ability to grow in 100 mM Ca²⁺ (but remained insensitive to 50 mM Sr²⁺) were identified in a screen of approximately 60,000 mutagenized yeast colonies. Cells carrying mutations in the CSG2 gene grow normally in low Ca²⁺ medium but have decreased growth rates when the Ca²⁺ concentration is above 10 mM. The csg2 mutant cells accumulate much higher levels of Ca²⁺ in a compartment that is exchangeable with extracellular Ca²⁺ but the nonexchangeable Ca²⁺ pool which predominates in wild-type cells is not influenced. Sr²⁺ influx is …

Regulation Of Cellular Ca2+ By Yeast Vacuoles, Teresa Dunn, Kenneth Gable, Troy Beeler

Uniformed Services University of the Health Sciences

The role of vacuolar Ca²⁺ transport systems in regulating cellular Ca²⁺ was investigated by measuring the vacuolar Ca²⁺ transport rate, the free energy available to drive vacuolar Ca²⁺ transport, the ability of the vacuole to buffer lumenal Ca²⁺, and the vacuolar Ca²⁺ efflux rate. The magnitude of the Ca²⁺ gradient generated by the vacuolar H⁺gradient best supports a 1 Ca²⁺:2 H⁺ coupling ratio for the vacuolar Ca²⁺/H⁺ exchanger. This coupling ratio along with a cytosolic Ca²⁺ concentration of 125 nM would give a …

Molecular Subtyping Of Salmonella Enteritidis Phage Type 8 Strains From The United States, Miguel A. Usera, Tanja Popovic, Cheryl A. Bopp, Nancy A. Strockbine

United States Public Health Resources

Salmonella enteridis is now the most common serotype of the genus Salmonella reported in the United States. Bacteriophage typing has been helpful for subdividing S. enteritidis strains from different sources in the United States. Most S. enteritidis outbreaks reported were egg related, and the majority of them were caused by strains of phage type 8. To determine whether restriction fragment length polymorphism of the rRNA genes (ribotyping) and of the genomic DNAs from two lysogenic phages from S. enteritidis could be used to discriminate between S. enteritidis phage type 8 strains, we conducted Southern hybridization studies on 24 isolates from …

Status Of Bovine Tuberculosis In North America, M. A. Essey, M. A. Koller

Michigan Bovine Tuberculosis Bibliography and Database

The eradication of bovine tuberculosis from North America is in the advanced stages as Canada and the United States struggle to remove the last vestiges of the disease from the domestic livestock population. Canada realistically anticipates total eradication from the national cattle herd within the next few years. The United States must yet effectively deal with the increased tuberculosis exposure potential from imported steers and from bovine tuberculosis newly discovered in its captive cervid industry. This paper reviews the history and development of tuberculosis eradication programs in North America. The basic evolution is described from area testing to slaughter surveillance …

The Role Of A Wildlife Reservoir In The Epidemiology Of Bovine Tuberculosis, D. U. Pfeiffer

Michigan Bovine Tuberculosis Bibliography and Database

The objective of this project was to study the epidemiology of bovine tuberculosis in the presence of a wildlife reservoir species. Cross-sectional and longitudinal studies of possum populations with endemic bovine tuberculosis infection were analyzed. The results were used to develop a computer simulation model of the dynamics of bovine tuberculosis infection in possum populations. A case-control study of breakdowns to tuberculosis infection in cattle herds in the Central North Island of New Zealand was conducted to identify risk factors other than exposure to tuberculosis in local possum populations.

The Specific Activities Of Shiga-Like Toxin Type Ii (Slt-Ii) And Slt-Ii-Related Toxins Of Enterohemorrhagic Escherichia Coli Differ When Measured By Vero Cell Cytotoxicity But Not By Mouse Lethality, Susanne W. Lindgren, James E. Samuel, Clare K. Schmitt, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Characteristically, enterohemorrhagic Escherichia coli (EHEC) strains produce Shiga-like toxin type I (SLT-I), SLT-II, or both of these immunologically distinct cytotoxins. No antigenic or receptor-binding variants of SLT-I have been identified, but a number of SLT-II-related toxins have been described. Because EHEC 091:H21 strain B2F1, which produces two SLT-II-related toxins, is exquisitely virulent in an orally infected, streptomycin-treated mouse model (oral 50% lethal dose [LD₅₀], <10 >organisms), we asked whether the pathogenicity of strain B2F1 was a consequence of SLT-II-related toxin production. For this purpose, we compared the lethality of orally administered E. coli DH5α(Str^r) strains that produced …

Cytotoxic Necrotizing Factor Type 2 Produced By Virulent Escherichia Coli Modifies The Small Gtp-Binding Proteins Rho Involved In Assembly Of Actin Stress Fibers, Eric Oswald, Motoyuki Sugai, Agnès Labigne, Henry C. Wu, Carla Fiorentini, Patrice Boquet, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Cytotoxic necrotizing factor type 2 (CNF2)produced by Escherichia coli strains isolated from intestinal and extraintestinal infections is a dermonecrotic toxin of 110 kDa. We cloned the CNF2 gene from a large plasmid carried by an Escherichia coli strain isolated from a lamb with septicemica. Hydropathy analysis of the deduced amino acid sequence revealed a largely hydrophilic protein with two peotential hydrophobic transmembrane domains. The N-terminal half of CNF2 showed striking homology (27% identity and 80% conserved residues) to the N-terminal portion of Pasteurella multocida toxin. Methylamine protection experiments and immunofluorescence studies suggested that CNF2 enters the cytosol of the target …

1994 Beef Cattle Report, Darrell W. Nelson

Nebraska Beef Cattle Reports

Two grazing trials were conducted to determine i/protein or energy was first limiting in the spring-and fall-born nursing calf grazing native sandhills range. Spring-born calves supplemented with escape protein gained more rapidly than calves supplemented with energy or nonsupplemented controls. Fall-born calves supplemented with escape protein gained more rapidly than those supplemented with rumen degradable protein or energy and nonsupplemented controls early in the trial, but gained at a similar rate to the energy controls by the end of the trial. Milk intake was similar across treatments for both trials. Escape protein is more limiting in the young nursing calf …

Morphometric Analysis Of Enteric Lesions In C3h/Hen Mice Inoculated With Serpulina Hyodysenteriae Serotypes 2 And 4 With Or Without Oral Streptomycin Pretreatment, Jagannatha V. Mysore, Gerald Duhamel

School of Veterinary and Biomedical Sciences: Faculty Publications

The segmental distribution and sequential progression and the role of the indigenous bacterial flora in the development of enteric lesions associated with Serpulina hyodysenteriae infection in laboratory mice have not been defined. We examined the distribution and sequential morphometric changes in the large intestine of mice orally inoculated with S. hyodysenteriae serotypes 2 and 4. To determine the role of colonization resistance conferred by the indigenous bacterial flora, 40 female C3H/HeN mice were administered water alone or water containing 5 mg/mL streptomycin sulfate ad libitum for seven days prior to orogastric inoculation either with S. hyodysenteriae or sterile trypticase soy …

Sanitation And Parasitism At Harpers Ferry, West Virginia, Karl J. Reinhard

Karl Reinhard Publications

One focus of historical archaeology at Harpers Ferry National Historical Park, West Virginia, is tracing the development of sanitation at the town through the 1800s and 1900s. Historical documentation indicates that there was a degree of resistance to the modernization of village sanitation. This study attempts to verify this resistance through examination of privy soils for parasites indicative of fecalborne disease, specifically the helminth species Ascaris lumbricoides and Trichuris trichiura. The presence of these parasites in the early 1900s would indicate that fecal-borne disease due to poor sanitation continued to be an aspect of town life. The analysis revealed the …

Otitis Media, Mastoiditis, And Infracranial Lesions In Two Plains Indian Children, Robert W. Mann, Douglas W. Owsley, Karl J. Reinhard

Karl Reinhard Publications

One disease condition that is common to nearly all children is otitis media, also known as middle ear disease. Although most people speak of it as a middle ear "infection," other causes such as neoplasm (tumor) can result in manifestations and symptoms mimicking an infectious process. This paper presents findings related to osseous lesions in two Indian children, approximately three to five years of age, who died with otitis media and infracranial lesions. Through macroscopic and radiographic analysis, it is possible to show that one child probably suffered from tuberculosis and the other from histiocytosis X. From an epidemiological and …

Trade, Contact, And Female Health In Northeast Nebraska, Karl Reinhard, Larry Tieszen, Karin L. Sandness, Lynae M. Beiningen, Elizabeth Miller, A. Mohammad Ghazi, Christiana E. Miewald, Sandra V. Barnum

Karl Reinhard Publications

Most scholars are understandably preoccupied with the impact of Europeans on native peoples who were passive, unwilling, or resistant participants in that contact. We present in this chapter a different case. The Missouri River tribes, including the Omaha and Ponca, willingly engaged in relations with Euramericans, especially in the fur trade that dominated interaction in this region. The time frame for this study is 1780- 1820, a period when interaction between individual traders and Native Americans was replaced by the dominance of the American Fur Company in organized exploitation of the Missouri River lands and peoples. This involvement later contributed …

“Introduction” To Integrative Views Of Motivation, Cognition, And Emotion, William D. Spaulding

Department of Psychology: Faculty Publications

One of my formative experiences as a graduate student came during a break in our afternoon-long first-year proseminar. We had been discussing the scope of psychology, or rather, listening to two professors debate the scope of psychology. Both the protagonists were clinical psychologists by training, but one had remained an empirically focused social learning theorist, while the other had pursued the study of Eastern religion, psychoanalysis, and transpersonal psychology. As the class milled about, the social learning empiricist sought some closure on the discussion. "Dick," he said to his transpersonal colleague, "it seems to me that we disagree because in …