Medicine and Health Sciences Commons^™

Promiscuous Mating In Feral Pigs (Sus Scrofa) From Texas, Usa, Johanna Delgado-Acevedo, Angeline Zamorano, Randy W. Deyoung, Tyler A. Campbell, David G. Hewitt, David B. Long

United States Department of Agriculture Wildlife Services: Staff Publications

Context. Feral pigs represent a significant threat to agriculture and ecosystems and are disease reservoirs for pathogens affecting humans, livestock and other wildlife. Information on the behavioural ecology of feral pigs might increase the efficiency and effectiveness of management strategies.

Aims. We assessed the frequency of promiscuous mating in relation to oestrous synchrony in feral pigs from southern Texas, USA, an agroecosystem with a widespread and well established population of feral pigs. An association between multiple paternity of single litters and synchrony of oestrous may indicate alternative mating strategies, such as mateguarding.

Methods. We collected gravid sows at …

Polishing Esthetic Restorative Materiai, Caren M. Barnes

College of Dentistry: Faculty Publications

When esthetic dental restorations are encountered during charting, they should be included in the treatment plan for the specific polishing procedures that are required. If the brand and type of esthetic restoration are known through the patient chart, the polishing procedures and products used should be those recommended by the manufacturer. If not possible, alternative polishing procedures should be used. A cleaning agent containing feldspar and rubber polishing cup can be used on all esthetic restorative materials with no chance of damage to the surface characterization.

The principles for polishing esthetic restorations are the same as for natural teeth. If …

Building The Genomic Base-Layer Of The Oral “Omic” World, Forsyth Metagenomic Support Consortium, Jacques Izard

Department of Food Science and Technology: Faculty Publications

With the shift of molecular technologies directed toward the understanding of greater biological complexity of the oral cavity, a knowledge gap was created by the lack of genomic data from the diverse oral microorganisms. To facilitate and enable the interpretation of metagenomic, transcriptomic, and proteomic data generated or soon to be generated from oral biofilms, we are providing reference genomic information from phylogenetically diverse oral bacterial isolates. This work, initiated by the National Institute of Dental and Craniofacial Research as an isolated effort, is now part of the Human Microbiome Project. The goal of this effort is the public release …

Neuroaids In Africa, Kevin Robertson, Jeff Liner, James Hakim, Jean-Louis Sankalé, Igor Grant, Scott Letendre, David Clifford, Amadou Gallo Diop, Assan Jaye, Georgette Kanmogne, Alfred Njamnshi, T. Dianne Langford, Tufa Gemechu Weyessa, Charles Wood, Mwanza Banda, Mina Hosseinipour, Ned Sacktor, Noeline Nakasuja, Paul Bangirana, Robert Paul, John Joska, Joseph Wong, Michael Boivin, Penny Holding, Betsy Kammerer, Annelies Van Rie, Prudence Ive, Avindra Nath, Kathy Lawler, Clement Adebamowo, Walter Royal Iii, Jeymohan Joseph

Nebraska Center for Virology: Faculty Publications

In July 2009, the Center for Mental Health Research on AIDS at the National Institute of Mental Health organized and supported the meeting “NeuroAIDS in Africa.” This meeting was held in Cape Town, South Africa, and was affiliated with the 5th IAS Conference on HIV Pathogenesis, Treatment and Prevention. Presentations began with an overview of the epidemiology of HIV in sub-Saharan Africa, the molecular epidemiology of HIV, HIV-associated neurocognitive disorders (HANDs), and HAND treatment. These introductory talks were followed by presentations on HAND research and clinical care in Botswana, Cameroon, Ethiopia, The Gambia, Kenya, Malawi, Nigeria, Senegal, South Africa, Uganda, …

Functional Properties Of The Hiv-1 Subtype C Envelope Glycoprotein Associated With Mother-To-Child Transmission, Hong Zhang, Marzena Rola, John T. West, Damien C. Tully, Piotr Kubis, Jun He, Chipepo Kankasa, Charles Wood

Nebraska Center for Virology: Faculty Publications

Understanding the properties of viruses capable of establishing infection during perinatal transmission of HIV-1 is critical for designing effective means of limiting transmission. We previously demonstrated that the newly transmitted viruses (in infant) were more fit in growth, as imparted by their envelope glycoproteins, than those in their corresponding mothers. Here, we further characterized the viral envelope glycoproteins from six mother-infant transmission pairs and determined whether any specific envelope functions correlate with HIV-1 subtype C perinatal transmission. We found that most newly transmitted viruses were less susceptible to neutralization by their maternal plasma compared to contemporaneous maternal viruses. However, the …

Chronology And Evolution Of The Hiv-1 Subtype C Epidemic In Ethiopia, Damien C. Tully, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objective—To reconstruct the onset date and evolutionary history of the HIV-1 subtype C epidemic in Ethiopia - one of the earliest recorded subtype C epidemics in the world.

Design—HIV-1 C env sequences with a known sampling year isolated from HIV-1 positive patients from Ethiopia between 1984 and 2003.

Methods—Evolutionary parameters including origin and demographic growth patterns were estimated using a Bayesian coalescent-based approach under either strict or relaxed molecular clock models.

Results—Bayesian evolutionary analysis indicated a most recent common ancestor date of 1965 with three distinct epidemic growth phases. Regression analysis of root-to-tip distances revealed a highly similar estimate for …

Enhancement Of Autophagy During Lytic Replication By The Kaposi’S Sarcoma-Associated Herpesvirus Replication And Transcription Activator, Hui-Ju Wen, Zhilong Yang, You Zhou, Charles Wood

Nebraska Center for Virology: Faculty Publications

Autophagy is one of two major degradation systems in eukaryotic cells. The degradation mechanism of autophagy is required to maintain the balance between the biosynthetic and catabolic processes and also contributes to defense against invading pathogens. Recent studies suggest that a number of viruses can evade or subvert the host cell autophagic pathway to enhance their own replication. Here, we investigated the effect of autophagy on the KSHV (Kaposi’s sarcoma-associated herpesvirus) life cycle. We found that the inhibition of autophagy reduces KSHV lytic reactivation from latency, and an enhancement of autophagy can be detected during KSHV lytic replication. In addition, …

Induction Of Interferon And Interferon Signaling Pathways By Replication Of Defective Interfering Particle Rna In Cells Constitutively Expressing Vesicular Stomatitis Virus Replication Proteins, Debasis Panda, Phat X. Dinh, Lalit Beura, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

We show here that replication of defective interfering (DI) particle RNA in HEK293 cells stably expressing vesicular stomatitis virus (VSV) replication proteins potently activates interferon (IFN) and IFN signaling pathways through upregulation of IFN- promoter, IFN-stimulated response element (ISRE) promoter, and NF-κB promoter activities. Replication of DI particle RNA, not mere expression of the viral replication proteins, was found to be critical for induction of IFN and IFN signaling. The stable cells supporting replication of DI RNA described in this report will be useful in further examining the innate immune signaling pathways and the host cell functions in viral genome …

Towards An Understanding Of The Herpes Simplex Virus Type 1 Latency-Reactivation Cycle, Guey-Chuen Perng, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Infection by herpes simplex virus type 1 (HSV-1) can cause clinical symptoms in the peripheral and central nervous system. Recurrent ocular shedding can lead to corneal scarring and vision loss making HSV-1 a leading cause of corneal blindness due to an infectious agent. The primary site of HSV-1 latency is sensory neurons within trigeminal ganglia. Periodically, reactivation from latency occurs resulting in virus transmission and recurrent disease. During latency, the latency-associated transcript (LAT) is abundantly expressed. LAT expression is important for the latency-reactivation cycle in animal models, in part, because it inhibits apoptosis, viral gene expression, and productive infection. A …

Method For Identification Of Virulence Determinants, Raul G. Barletta, Beth Harris

School of Veterinary and Biomedical Sciences: Faculty Publications

Disclosed are methods for the determination of virulence determinants in bacteria and in particular bacteria of the genus Mycobacterium. Also disclosed are compositions and methods for stimulating an immune response in an animal using bacteria and virulence determinants identified by the methods of the present invention.

A Deletion Mutation In Bovine Slc4a2 Is Associated With Osteopetrosis In Red Angus Cattle, Stacey N. Meyers, Tara G. Mcdaneld, Shannon L. Swist, Brandy M. Marron, David J. Steffen, Donal O'Toole, Jeffrey R. O'Connell, Jonathan E. Beever, Tad S. Sonstegard, Timothy P.L. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Osteopetrosis is a skeletal disorder of humans and animals characterized by the formation of overly dense bones, resulting from a deficiency in the number and/or function of bone-resorbing osteoclast cells. In cattle, osteopetrosis can either be induced during gestation by viral infection of the dam, or inherited as a recessive defect. Genetically affected calves are typically aborted late in gestation, display skull deformities and exhibit a marked reduction of osteoclasts. Although mutations in several genes are associated with osteopetrosis in humans and mice, the genetic basis of the cattle disorder was previously unknown.

Results: We have conducted a whole-genome …

Increased Dendrite Branching In Aβpp/Ps1 Mice And Elongation Of Dendrite Arbors By Fasudil Administration, Brian A. Couch, George J. Demarco, Shannon L. Gourley, Anthony J. Koleske

School of Biological Sciences: Faculty Publications

Amyloid-β (Aβ) overproduction and dendrite arbor atrophy are hallmarks of Alzheimer’s disease. The RhoA GTPase (Rho) signals through Rho kinase (ROCK) to control cytoskeletal dynamics and regulate neuron structure. Hyperactive Rho signaling destabilizes neurons leading to dendritic regression that can be rescued by genetic or pharmacological reduction of ROCK signaling. To understand what effect reduced ROCK signaling has on the dendrite arbors of mice that overproduce Aβ, we administered the ROCK inhibitor fasudil to AβPP/PS1 transgenic mice. We report that increased dendrite branching occurs in AβPP/PS1 mice and that fasudil promotes …

Synaptic Clustering Of Psd-95 Is Regulated By C-Abl Through Tyrosine Phosphorylation, Karen Perez De Arce, Lorena Varela-Nallar, Olivia Farias, Alejandra Cifuentes, Paulina Bull, Brian A. Couch, Anthony J. Koleske, Nibaldo C. Inestrosa, Alejandra R. Alvarez

School of Biological Sciences: Faculty Publications

The c-Abl tyrosine kinase is present in mouse brain synapses, but its precise synaptic function is unknown. We found that c-Abl levels in the rat hippocampus increase postnatally, with expression peaking at the first postnatal week. In 14 d in vitro hippocampal neuron cultures, c-Abl localizes primarily to the postsynaptic compartment, in which it colocalizes with the postsynaptic scaffold protein postsynaptic density protein-95 (PSD-95) in apposition to presynaptic markers. c-Abl associates with PSD-95, and chemical or genetic inhibition of c-Abl kinase activity reduces PSD-95 tyrosine phosphorylation, leading to reduced PSD-95 clustering and reduced synapses in treated neurons. c-Abl can phosphorylate …

Purification Of Parvalbumin From Carp: A Protocol That Avoids Heat Treatment, Stef J. Koppelman, Roland A. Romijn, Harmen H. J. De Jongh, Julie A. Nordlee, Sander Piersma, Martin Hessing, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Parvalbumin from carp, a major allergen,was purified to homogeneity using ion exchange chromatography and size exclusion chromatography (estimated purity > 95% to 98% based on SDS-PAGE and native PAGE) with a yield of 318 mg, and a number of basic biochemical characteristics were determined. The identity was confirmed by peptide-mass fingerprinting, and IgE-binding was demonstrated. The UV/Vis absorbance spectra were explained using the previously published amino acid sequences. Far UV-CD spectroscopy was used to confirm the folding character of parvalbumin. We conclude that parvalbumin from carp can be purified on a comparatively large (hundreds of milligrams) scale using a purification protocol …

Vowel Recognition From Continuous Articulatory Movements For Speaker-Dependent Applications, Jun Wang, Jordan R. Green, Ashok Samal, Tom D. Carrell

Department of Special Education and Communication Disorders: Faculty Publications

A novel approach was developed to recognize vowels from continuous tongue and lip movements. Vowels were classified based on movement patterns (rather than on derived articulatory features, e.g., lip opening) using a machine learning approach. Recognition accuracy on a single-speaker dataset was 94.02% with a very short latency. Recognition accuracy was better for high vowels than for low vowels. This finding parallels previous empirical findings on tongue movements during vowels. The recognition algorithm was then used to drive an articulation-to-acoustics synthesizer. The synthesizer recognizes vowels from continuous input stream of tongue and lip movements and plays the corresponding sound samples …

Iptakalim: A Potential Antipsychotic Drug With Novel Mechanisms?, Tao Sun, Changjiu Zhao, Gang Hu, Ming Li

Department of Psychology: Faculty Publications

Iptakalim is a novel putative adenosine triphosphate (ATP)-sensitive potassium (K_ATP) channel opener. In the brain, iptakalim is thought to act on the neuronal and astrocytic plasma membrane and/or mitochondrial K_ATP channels. Because iptakalim demonstrates an action on the regulation of dopamine and glutamate release in the forebrain regions, we examined its potential antipsychotic efficacy in several preclinical tests. First, we show that iptakalim is effective in reducing amphetamine- and phencyclidine-induced hyperlocomotion as well as selectively disrupting conditioned avoidance responding. Next, we show that combined iptakalim and amphetamine treatment produces a reduction on prepulse inhibition of acoustic startle …

Punished, Dead Or Alive: Empirical Perspectives On Awarding Punitive Damages Against Deceased Defendants, Timothy R. Robicheaux, Brian H. Bornstein

Department of Psychology: Faculty Publications

Punitive damages are a tool for punishing defendants who engage in reckless and wanton behaviors that cause injury to others. As with criminal punishment, goals of punitive damages include retribution, specific deterrence, and general deterrence. Unlike criminal punishment, however, some courts allow punitive damages to follow the death of defendants. To explore this issue, we first conducted a legal analysis of appellate court decisions concerning punitive damages against deceased defendants. While the majority of courts suggest that punitive damages against deceased individuals are not appropriate, some argue that favorable policy implications of allowing punitive damages to follow the defendant’s death …

Aging And The Vulnerability Of Speech To Dual Task Demands, Susan Kemper, Ralynn Schmalzried, Lesa Hoffman, Ruth Herman

Department of Psychology: Faculty Publications

Tracking a digital pursuit rotor task was used to measure dual task costs of language production by young and older adults. Tracking performance by both groups was affected by dual task demands: time on target declined and tracking error increased as dual task demands increased from the baseline condition to a moderately demanding dual task condition to a more demanding dual task condition. When dual task demands were moderate, older adults’ speech rate declined but their fluency, grammatical complexity, and content were unaffected. When the dual task was more demanding, older adults’ speech, like young adults’ speech, became highly fragmented, …

Donor Payoffs And Other-Regarding Preferences In Cotton-Top Tamarins (Saguinus Oedipus), Jeffrey R. Stevens

Department of Psychology: Faculty Publications

Helping others at no cost to oneself is a simple way to demonstrate other-regarding preferences. Yet, primates exhibit mixed results for other-regarding preferences: chimpanzees and tamarins do not show these effects, whereas capuchin monkeys and marmosets preferentially give food to others. One factor of relevance to this no-cost food donation is the payoff to the donor. Though donors always receive the same payoffs regardless of their choice, previous work varies in whether they receive either a food reward or no food reward. Here, I tested cotton-top tamarins in a preferential giving task. Subjects could choose from two tools, one of …

The Adaptive Nature Of Impulsivity, Jeffrey R. Stevens, David W. Stephens

Department of Psychology: Faculty Publications

An old joke circulates among animal behavior instructors. One can, the joke goes, divide the topics of animal behavior into four Fs: fighting, fleeing, feeding, and reproduction. This somewhat tired joke carries considerable truth. Animals behaving in nature surely must make decisions about conflicts, predator avoidance, feeding, and mating. Male crickets, for example, are notoriously combative. Studies have shown, however, that they escalate fights in some situations and retreat to fight another day in others (Beaugrand, 1997; Parker, 1974; Parker & Rubenstein, 1981). Squirrels, like many small animals, respond to the presence or absence of protective cover; for example, they …

Intertemporal Choice, Jeffrey R. Stevens

Department of Psychology: Faculty Publications

A Clark’s nutcracker (Nucifraga columbiana) flies to the top of a pine tree and selects one of the many cones. She twists and pecks at the stem until the cone breaks free from the branch and, with one foot, holds the cone in the crotch of a branch. She then repeatedly hammers her long bill in between the scales of the cone. After forcing out one of the seeds, she tips her head back a bit, clicks the seed in her bill a few times, and closes her bill. A few minutes later, the shredded cone drops to …

Rational Decision Making In Primates: The Bounded And The Ecological, Jeffrey R. Stevens

Department of Psychology: Faculty Publications

Primates constantly face decisions that influence their survival and reproduction. Continue foraging in this tree or move on to another? Expose oneself to a hidden predator by straying from the group or enjoy the safety of having other potential victims nearby? Defend one’s territory from invaders or abandon it and seek a new home? In all of these cases, primates must trade off the costs and benefits associated with uncertain and delayed decision outcomes. The outcomes of these choices influence survival and reproduction, and natural selection should favor those individuals whose choices lead to the propagation of their genes.

The …

Physical, Psychological, And Sexual Intimate Partner Aggression Among Newlywed Couples: Longitudinal Prediction Of Marital Satisfaction, Jillian Panuzio, David K. Dilillo

Department of Psychology: Faculty Publications

This study examined associations between physical, psychological, and sexual intimate partner aggression (IPA) perpetration during the first year of marriage (T1) and victim marital satisfaction one (T2) and two (T3) years later among a sample of 202 newlywed couples. Prevalence rates of all forms of IPA were consistent with those documented in prior research. Higher levels of all types of IPA generally were associated with lower victim marital satisfaction at all time points, when controlling for initial levels of satisfaction. Couples who reported severe bidirectional psychological IPA demonstrated lower husband and wife marital satisfaction at T2 and lower husband satisfaction …

Development And Initial Psychometric Properties Of The Computer Assisted Maltreatment Inventory (Cami): A Comprehensive Self-Report Measure Of Child Maltreatment History, David K. Dilillo, Sarah A. Hayes-Skelton, Michelle A. Fortier, Andrea R. Perry, Sarah E. Evans, Terri L. Messman Moore, Kate Walsh, Cindy Nash, Angèle Fauchier

Department of Psychology: Faculty Publications

Objectives: The present study reports on the development and initial psychometric properties of the Computer Assisted Maltreatment Inventory (CAMI), a web-based self-report measure of child maltreatment history, including sexual and physical abuse, exposure to interparental violence, psychological abuse, and neglect.

Methods: The CAMI was administered to a geographically diverse sample of college students (N= 1,398). For validation purposes, participants also completed a widely used measure of maltreatment (Childhood Trauma Questionnaire) as well as measures of social desirability. To examine test–retest reliability, a subset of participants (n = 283) completed the CAMI a second time 2–4 weeks after …

Emotion Dysregulation And Risky Sexual Behavior In Revictimization, Terri L. Messman-Moore, Kate L. Walsh, David K. Dilillo

Department of Psychology: Faculty Publications

Objective: The current study examined emotion dysregulation as a mechanism underlying risky sexual behavior and sexual revictimization among adult victims of child sexual abuse (CSA) and child physical abuse (CPA).

Methods: Participants were 752 college women. Victimization history, emotion dysregulation, and risky sexual behavior were assessed with anonymous, self-report surveys utilizing a cross-sectional design.

Results: Approximately 6.3% of participants reported CSA, 25.5% reported CPA, and 17.8% reported rape during adolescence or adulthood. CSA and CPA were associated with increased risk for adolescent/adult rape; 29.8% of CSA victims and 24.3% of CPA victims were revictimized. Path analytic models tested hypothesized relationships …

Research Methodology For Validation Of Nutrition Diagnostic Standardized Language Using Fehring Models, Paula K. Ritter-Gooder, Nancy M. Lewis

Department of Nutrition and Health Sciences: Faculty Publications

Validation of the nutrition standardized language (SL) confirms the language is accurate for use in practice, research, and policy. Nutrition diagnostic terms are identified by concept analysis using literature review and experts to distinguish and differentiate one nutrition problem from a similar or related problem. The Fehring models, used in nursing diagnosis validation research, are adopted to validate the SL nutrition diagnostic terms using experts and weighted inter-rater reliability ratios. In the first phase of validation research, diagnostic terms are validated by experts using the Diagnostic Content Validity Model (DCV). A Nutrition Diagnosis Validation Instrument (NDVI) is developed that contains …

A Preliminary Study: Body Dysmorphic Disorder In Division I Women’S Collegiate Soccer Players, Tammy D. Jones

Department of Educational Administration: Dissertations, Theses, and Student Research

The prevalence of Body Dysmorphic Disorder (BDD) among collegiate athletes has not been clearly determined. The purpose of this study was to determine if there are symptoms of body dysmorphic disorder found in Division I women’s soccer players. The researcher hypothesized that there would be some symptoms found within the participants of sport and that there was a need to research this area further.

The study consisted of four participants who participated in semi-structured interviews. The subjects were asked a series of questions from the Yale-Brown Obsessive Compulsive Scale Modified for Body Dysmorphic Disorder (BDD-YBOCS) to determine if they indicated …

T Cell Receptor Cross-Reactivity Directed By Antigen-Dependent Tuning Of Peptide-Mhc Molecular Flexibility, Oleg Y. Borbulevych, Kurt H. Piepenbrink, Brian E. Gloor, Daniel R. Scott, Ruth F. Sommese, David K. Cole, Andrew K. Sewell, Brian M. Baker

Food for Health: Publications

Tell mediated immunity requires T cell receptor (TCR) cross-reactivity, the mechanisms behind which remain incompletely elucidated. The αβ TCR A6 recognizes both the Tax (LLFGYPVYV) and Tel1p (MLWGYLQYV) peptides presented by the human class I MHC molecule HLA-A2. Here we found that although the two ligands are ideal structural mimics, they form substantially different interfaces with A6, with conformational differences in the peptide, the TCR, and unexpectedly, the MHC molecule. The differences between the Tax and Tel1p ternary complexes could not be predicted from the free peptide-MHC structures and are inconsistent with a traditional induced-fit mechanism. Instead, the differences were …

Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi Reynolds, Pamela Sheaff

Biomedical Imaging and Biosignal Analysis Laboratory

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation Without interference from unincorporated NPs and Without illuminating the polymerase-DNA complex.

Modified Head Shake Computerized Dynamic Posturography, Julie A. Honaker, Connie M. Converse, Neil T. Shepard

Department of Special Education and Communication Disorders: Faculty Publications

PURPOSE: Recent research on head shake posturography has demonstrated a modest increase in sensitivity to identifying peripheral vestibular system asymmetry when horizontal head movements were added to portions of the standard Sensory Organization Test (SOT) battery. However, limitations with respect to the head shake protocol were outlined, and usable data for assessing performance could not be established. The purpose of this study was to test a change in protocol for use of head shake SOT to address the noted limitations.
METHOD: Forty participants ranging in age from 20 to 79 years with no history of dizziness completed Conditions 2 and …