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University of Nebraska - Lincoln

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Full-Text Articles in Medicine and Health Sciences

Networking With Researchers And Farmers To Tackle Herbicide-Resistant Weeds, Melinda K. Yerka Sep 2016

Networking With Researchers And Farmers To Tackle Herbicide-Resistant Weeds, Melinda K. Yerka

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Outline •Crop breeding technology •Regulation of crop technologies •Issues related to crop-to-weed gene flow •Need for landscape-level studies

GMO •Genetically Modified Organism •Most commercial traits facilitate crop production –Insect resistance (Btcorn) –Herbicide resistance (Roundup) •General scientific consensus that they are safe •Public concerns persist

GM…..Oh? •GMOs are regulated substances by USDA-APHIS –$80 to $150 million per genotype to get to market •USDA-APHIS: Which biotech traits do we regulate? •Commodity groups: Will we be regulated???

The special case of sorghum: Massive international development as a biofuel •Better drought tolerance than corn •Needs herbicide resistance to compete
Evolution of herbicide resistance in …


Why We Need To Help Youth Develop A Stem Identity, Saundra Frerichs Sep 2016

Why We Need To Help Youth Develop A Stem Identity, Saundra Frerichs

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Favorite Toys

Developing Identity

Click 2 Science http://click2sciencepd.org

click2sciencepd.org

[email protected]

@Click2Science


Training Undergraduates To Sell It: #Scicomm In An Ecology Course, Teresa Bilinski Sep 2016

Training Undergraduates To Sell It: #Scicomm In An Ecology Course, Teresa Bilinski

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Stop. Being. Boring.

Science: Soft Skill? No.

Essential Transferable Skill: “Students would be better served by an education that is integrated with the job market they will encounter post-graduation, and one that provides not only technical skills but also the soft skills that are most in demand by employers such as communication…”

#SciCommin a senior Ecology course: Course-based undergraduate research experience (CURE). Ecosystem services at the wildland-urban interface: involving undergraduates in ecological investigations

Pre- assignment “Summarize the Results of an ecological research project related to the effects of urbanization on ecosystems. The research summaries will be presented orally to the …


The Engineering Ambassadors Network: Engaging Undergraduate Engineers In P-12 Outreach, Sally Wei Sep 2016

The Engineering Ambassadors Network: Engaging Undergraduate Engineers In P-12 Outreach, Sally Wei

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

In the next few minutes I want to focus your attention on the T and E of STEM. Take a few seconds and think about what in this room is either not engineering and tech itself or does not have any interface with engineering and tech.

So engineering is ubiquitous, how do we teach this across society particularly P12 and how do we create the pipeline?

The Engineering Ambassadors Network is a nationwide network of colleges and universities designed to change the conversation on engineering.

EAN’s mission is two‐fold: to develop engineering leaders as well as impact P‐12 engineering education. …


How To Be A Biologist?, Fernando Mateos-González Sep 2016

How To Be A Biologist?, Fernando Mateos-González

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

http://bioblogia.net

http://www.bioblogia.net/p/consejosjovenesbiologos.html

Tips for young biologists-- •How and why study Biology •How to get experience, contacts, and jobs as a biologist •My own troubles/experiences

What do they ask about?

My own goals for SciComm2016…


Science Communication Strategies In The K-12 Classroom, Andrew Ising Sep 2016

Science Communication Strategies In The K-12 Classroom, Andrew Ising

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

@ItsIsing

andrewising@gmail

KABT.org #SciComm2016

bit.ly/isingnsc

Why is science literacy important to you? Why is science literacy important to me?

Situation #1: Peer Review -- Scientists talking to other Scientists; Making Science Research More Accessible: Poster Sessions [KABT Miniposters]

Situation #2: Informal Science -- Scientists telling science stories Common sources: Blogs, science magazines, newspapers, etc. Documentaries Social Media Why students like this: Narrative… Drama… Fewer long latin words

Situation #3: Community Outreach -- Scientists talking as experts to an audience “A scientist seen is a scientist trusted” Be/Become someone’s “BROADER IMPACTS”! Citizen Science Projects (Scistarter, YourWildLife, IMP, Great KS Tardigrade Hunt, …


Eight-Legged Encounters: Using Organismal Biology To Bring Science Education To Families & Communities, Eileen A. Hebets Sep 2016

Eight-Legged Encounters: Using Organismal Biology To Bring Science Education To Families & Communities, Eileen A. Hebets

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Path of the Predators: A Journey Through the Living Arachnids. Illustrations by Pawl Tisdale

WHAT IS AN ARTHROPOD?

CREATE A CHELICERATE

ASSEMBLE AN ARACHNID

TISSUE PAPER FLOWER

MICROSCOPE MADNESS

Eight-Legged Encounters Impact

Change in interest in learning about scientific discoveries among adult respondents (n = 63).

Youth’s interest in science activities (n = 42). Respondents were 50% female, 50% male and ranged in age from 4 – 14 with a mean of 7.7.


Building Educator Capacity To Address Animal Agriculture In A Changing Climate, Crystal Powers Sep 2016

Building Educator Capacity To Address Animal Agriculture In A Changing Climate, Crystal Powers

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Multi-Regional Approach.

Project Support.

Project Objectives • Equip Educators –ability & motivation • Provide science-based information • Coordinate state, regional and national levels.

Online Course. Outcomes -ability. Outcomes -motivation.

Understand worldviews. Meet people where they are. Start with local history.

Framing: Focus on audience’s values & concerns. Present new information so it can be heard

Science in Action: • Public wants to know how the science applies to their life. • We can point back to the basic science

SciComm Lessons Learned


Engineering Empowerment: Science Literacy Through Engineering Design, Jennifer R. Keshwani Sep 2016

Engineering Empowerment: Science Literacy Through Engineering Design, Jennifer R. Keshwani

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Outcome A scientifically literate society capable of making effective decisions grounded in STEMinformed analyses of complex, real‐world challenges associated with food, fuel, water, landscape, and people issues.

Finding Solutions for Life on a Small Planet

Produce future STEM professionals

Develop a science and technology literate society

K-12 Engineering: Exposure to Engineering Design; K‐12 Engineering Design Curricula; Undergrad Teaching Experience; K‐12 Educator Professional Development


The Role Of Design Thinking In Scientific Research & Communication, Colleen Syron Sep 2016

The Role Of Design Thinking In Scientific Research & Communication, Colleen Syron

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Design thnking: Research: • Interviews with designers • Observations and case studies • Experimental studies • Simulation • Reflection and theorizing

Designing is not a search for the optimum solution to the given problem, but that it is an exploratory process. The creative designer interprets the design brief not as a specification for a solution, but as a starting point for a journey of exploration,…

“Deduction proves that something must be; Induction shows that something actually is operative; Abduction suggests that something may be.” Charles Pierce

The main point of difference is that of timing. Both artists and scientists operate …


Public Perceptions Of Drones Used For Weather-Related Purposes, Lisa M. Pytlikzillig, Janell C. Walther, Carrick Detweiler, Adam L. Houston Sep 2016

Public Perceptions Of Drones Used For Weather-Related Purposes, Lisa M. Pytlikzillig, Janell C. Walther, Carrick Detweiler, Adam L. Houston

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

The CLOUDMAP Team -- Collaboration Leading Operational UAS Development for Meteorology and Atmospheric Physics. An EPSCOR grant funded by NSF

Responsible Innovation & Trust

Public Perception Studies to Date

Some Major Findings • You can call a drone whatever you want without changing people’s support • Purpose matters • Trust matters…

And More Findings • Sensemaking through pop culture, lack of knowledge, questioning, and purpose. • Hopes for societal benefits such as research technology and improved public safety (reduced risk, better forecasting). • Concerns for privacy, public & airspace safety, and pollution. • Recommendations for collaborative regulation creation for safety …


You Kan: A Graduate Student Perspective On K-12 Outreach In Ks, Nicole Green Sep 2016

You Kan: A Graduate Student Perspective On K-12 Outreach In Ks, Nicole Green

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Science gets interesting at interfaces.

K-12 Outreach in Kansas:
NSF GK-12 EIDROP Fellow (2014-2015) #0841414 Partnership with USD 475 ‘Resident Scientist’, AP Biology
EXCITE! Summer Workshop Series (2015, 2016) KAWSE Office ‘Drosophi-what? Using Fruit Flies as a Biological Model Organism’, 9th-12th grades
4-H SPecial INterest (SPIN) Fall 2015-Spring 2016 After school programming with USD 383, 2nd-5thgrades
Camp R.O.A.R. (Real Observation ARoundUs), Lincoln Elementary School, K-5th grades, funded by ASCB COMPASS Outreach grant

Engaging in outreach is a unique graduate training experience. Successful outreach programs meet students where they are.

If you walk into the classroom with good intentions and a …


Structural Diversity In The Type Iv Pili Of Multidrug-Resistant Acinetobacter, Kurt H. Piepenbrink, Erik Lillehoj, Christian M. Harding, Jason W. Labonte, Xiaotong Zuo, Chelsea A. Rapp, Robert S. Munson Jr, Simeon E. Goldblum, Mario F. Feldman, Jeffrey J. Gray, Eric J. Sundberg Sep 2016

Structural Diversity In The Type Iv Pili Of Multidrug-Resistant Acinetobacter, Kurt H. Piepenbrink, Erik Lillehoj, Christian M. Harding, Jason W. Labonte, Xiaotong Zuo, Chelsea A. Rapp, Robert S. Munson Jr, Simeon E. Goldblum, Mario F. Feldman, Jeffrey J. Gray, Eric J. Sundberg

Food for Health: Publications

Acinetobacter baumannii is a Gram-negative coccobacillus found primarily in hospital settings that has recently emerged as a source of hospital-acquired infections. A. baumannii expresses a variety of virulence factors, including type IV pili, bacterial extracellular appendages often essential for attachment to host cells. Here, we report the high resolution structures of the major pilin subunit, PilA, from three Acinetobacter strains, demonstrating thatA. baumannii subsets produce morphologically distinct type IV pilin glycoproteins. We examine the consequences of this heterogeneity for protein folding and assembly as well as host-cell adhesion by Acinetobacter. Comparisons of genomic and structural data with pilin proteins from …


Temporal And Spatial Distribution Of Enterobius Vermicularis (Nematoda: Oxyuridae) In The Prehistoric Americas, Karl J. Reinhard, Adauto Araújo, Johnica J. Morrow Sep 2016

Temporal And Spatial Distribution Of Enterobius Vermicularis (Nematoda: Oxyuridae) In The Prehistoric Americas, Karl J. Reinhard, Adauto Araújo, Johnica J. Morrow

Karl Reinhard Publications

Investigations of Enterobius sp. infection in prehistory have produced a body of data that can be used to evaluate the geographic distribution of infection through time in the Americas. Regional variations in prevalence are evident. In North America, 119 pinworm positive samples were found in 1,112 samples from 28 sites with a prevalence of 10.7%. Almost all of the positive samples came from agricultural sites. From Brazil, 0 pinworm positive samples were found in 325 samples from 7 sites. For the Andes region, 22 pinworm positive samples were found in 411 samples from 26 sites for a prevalence of 5.3%. …


Nonmetropolitan Nebraskans’ Perceptions Of Local Schools: 2016 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L.J. Mcelravy, Jeanne Lorraine Surface Sep 2016

Nonmetropolitan Nebraskans’ Perceptions Of Local Schools: 2016 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L.J. Mcelravy, Jeanne Lorraine Surface

Rural Futures Institute: Publications

Conclusion

Most rural Nebraskans have elementary, middle and high schools in their community or nearest community. Most of the schools available are public but many rural Nebraskans report having both public and private schools of each grade level. Persons living in or near larger communities are more likely than persons living in or near smaller communities to have each level of school in their community. And, persons living in or near the larger communities are more likely than persons living in or near smaller communities to have both public and private schools available.

When asked about priorities for their local …


The Neu1-Selective Sialidase Inhibitor, C9- Butyl-Amide-Dana, Blocks Sialidase Activity And Neu1-Mediated Bioactivities In Human Lung In Vitro And Murine Lung In Vivo, Sang W. Hyun, Anguo Liu, Zhenguo Liu, Alan S. Cross, Avelino C. Verceles, Sadagopan Magesh, Yadagiri Kommagalla, Chandrababunaidu Kona, Hiromune Ando, Irina G. Luzina, Sergei P. Atamas, Kurt H. Piepenbrink, Eric J. Sundberg, Wei Guang, Hideharu Ishida, Erik P. Lillehoj, Simeon E. Goldblum Sep 2016

The Neu1-Selective Sialidase Inhibitor, C9- Butyl-Amide-Dana, Blocks Sialidase Activity And Neu1-Mediated Bioactivities In Human Lung In Vitro And Murine Lung In Vivo, Sang W. Hyun, Anguo Liu, Zhenguo Liu, Alan S. Cross, Avelino C. Verceles, Sadagopan Magesh, Yadagiri Kommagalla, Chandrababunaidu Kona, Hiromune Ando, Irina G. Luzina, Sergei P. Atamas, Kurt H. Piepenbrink, Eric J. Sundberg, Wei Guang, Hideharu Ishida, Erik P. Lillehoj, Simeon E. Goldblum

Food for Health: Publications

Neuraminidase-1 (NEU1) is the predominant sialidase expressed in human airway epithelia and lung microvascular endothelia where it mediates multiple biological processes. We tested whether the NEU1-selective sialidase inhibitor, C9-butyl-amide-2-deoxy-2,3-dehydro-Nacetylneuraminic acid (C9-BA-DANA), inhibits one or more established NEU1-mediated bioactivities in human lung cells. We established the IC50 values of C9-BA-DANA for total sialidase activity in human airway epithelia, lung microvascular endothelia and lung fibroblasts to be 3.74 µM, 13.0 µM and 4.82 µM, respectively. In human airway epithelia, C9-BA-DANA dose-dependently inhibited flagellin-induced, NEU1-mediated mucin-1 ectodomain desialylation, adhesiveness for Pseudomonas aeruginosa and shedding. In lung microvascular endothelia, C9-BA-DANA reversed NEU1-driven restraint of …


Experimental Infection Of Conventional Nursing Pigs And Their Dams With Porcine Deltacoronavirus, Sarah Vitosh-Sillman, John Dustin Loy, Bruce W. Brodersen, Clayton L. Kelling, Alan R. Doster, Christina L. Topliff, Eric A. Nelson, Jianfa Bai, Erin Schirtzinger, Elizabeth Poulsen, Barbara Meadors, Joseph Anderson, Benjamin Hause, Gary Anderson, Richard Hesse Sep 2016

Experimental Infection Of Conventional Nursing Pigs And Their Dams With Porcine Deltacoronavirus, Sarah Vitosh-Sillman, John Dustin Loy, Bruce W. Brodersen, Clayton L. Kelling, Alan R. Doster, Christina L. Topliff, Eric A. Nelson, Jianfa Bai, Erin Schirtzinger, Elizabeth Poulsen, Barbara Meadors, Joseph Anderson, Benjamin Hause, Gary Anderson, Richard Hesse

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine deltacoronavirus (PDCoV) is a newly identified virus that has been detected in swine herds of North America associated with enteric disease. The aim of this study was to demonstrate the pathogenicity, course of infection, virus kinetics, and aerosol transmission of PDCoV using 87 conventional piglets and their 9 dams, including aerosol and contact controls to emulate field conditions. Piglets 2–4 days of age and their dams were administered an oronasal PDCoV inoculum with a quantitative real-time reverse transcription (qRT)-PCR quantification cycle (Cq) value of 22 that was generated from a field sample having 100% nucleotide identity to USA/Illinois121/2014 determined …


Neural Control Of Tongue Movements Across Effort Levels, Megan Rovang, Angela M. Dietsch Sep 2016

Neural Control Of Tongue Movements Across Effort Levels, Megan Rovang, Angela M. Dietsch

UCARE: Research Products

Background

A hallmark of Parkinson’s disease (PD) is a mismatch between the perceived effort and actual forces exerted during functional activities such as speech. Current evidence supports therapy to help reset this perception of effort, but the neurological underpinnings of such treatments are unclear. This study examined brain activity during tongue movements performed at varying levels of effort to determine (1) which brain areas are involved in each task, and (2) which areas, if any, scale in activation according to effort level. These results, considered with the neurological changes associated with PD, can be used to develop and refine treatment …


Cleaning Puparia For Forensic Analysis, Leon G. Higley, Tierney R. Brosius, Karl Reinhard, David Carter Sep 2016

Cleaning Puparia For Forensic Analysis, Leon G. Higley, Tierney R. Brosius, Karl Reinhard, David Carter

Karl Reinhard Publications

We tested procedures for removing adipocere from insect samples to allow identification. An acceptable procedure was determined: (i) Samples were sorted in petri dishes with 75% alcohol to remove any larvae, adult insects, or other soft-bodied material. (ii) Samples of up to 24 puparia were placed in a vial with 15 mL of 95% acetone, capped, and vortexed for a total of 30–90 sec in 10- to 15-sec bursts. This step removed large masses of adipocere or soil from specimen. (iii) Specimens were removed from acetone and placed in a vial of 15 mL of 2% potassium hydroxide (KOH) and …


Raising Nebraska: Engaging The Public In Ways That They Value, Chuck Hibberd Aug 2016

Raising Nebraska: Engaging The Public In Ways That They Value, Chuck Hibberd

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Raising Nebraska A partnership: •Nebraska State Fair •Nebraska Extension •Nebraska Department of Agriculture With a purpose: •Demonstrate the science and art of Nebraska agriculture •Learn from consumers

Prescribing irrigation –conserving water

Walkable Nebraska map -elevation change, waterways, precipitation and crops/livestock

Trusted Voice Videos –Top 5 & Bottom 5

Interactive Dinner Table -- Dinner Table –Entrée, Drink, & Side Selection

Exit Poll -How many things did you learn today?

Conclusions •Participants are telling us what they want to learn more about •Participant responses provide insight on content offerings •Interactive, digitally-based learning venues are effective


Tell Me A Science Story: Transforming Engagement With Science Using Creative Nonfiction, Janell C. Walther Aug 2016

Tell Me A Science Story: Transforming Engagement With Science Using Creative Nonfiction, Janell C. Walther

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

“I definitely felt more engaged with the first one [creative nonfiction]and less tuned out because I had somebody interpreting their response for me. So when he was like “Oh, I was surprised” then I think “Oh, so was I, I was also surprised by the piece of information.” So I was more engaged with it personally even if I didn't retain all the numbers... “

“[The story] seemed more relatable I guess, as a normal, average, everyday person than a whole bunch of scientists talking about facts. Where the facts were still in here, the way that it was written …


Science Not Communicated Is Science Not Done: Present Your Science Powerfully, Melissa Marshall Aug 2016

Science Not Communicated Is Science Not Done: Present Your Science Powerfully, Melissa Marshall

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

Great communication ignites a chain reaction. Discussion: Identify the best presenter of science that you have seen. What made that speaker so effective?

To excel in your presentations, you will need content , passion, and a sharp sense of the audience.

Science not communicated is science not done. Presenting your science powerfully requires mastery of three skills: Be Audience Centered, Filter and focus, Show your science

Becoming an audience focused speaker is one of the most powerful skills you can adopt. Returning to points of common ground satisfies audiences of different technical backgrounds.

Successful technical presentations require you to filter …


How To Talk About Science In The Face Of Religious Mistrust, Clay Farris Naff Aug 2016

How To Talk About Science In The Face Of Religious Mistrust, Clay Farris Naff

SciComm 2016 - Lincoln, Nebraska, September 23-24, 2016

King Ashoka (268to 232 BCE). Ralph Waldo Emerson. Homer Simpson. Us and THEM. Timothy Gay, UNL Prof. of Physics. Ken Miller, Prof. of Biology, Brown. Richard Dawkins. Ken Ham, Answers in Genesis.

THEM = Truthful Humble Evidential Memorable

Theistic Evolution. Human Babies Barely Fit. The right & left laryngeal nerves take wildly differing paths. Progeria. General Theory of Relativity. Sun’s Gravity Bends Starlight. GPS & Relativity. Sugar vs. Fat. U.S. stock market. Pope. Dalai Lama. Epicurus.


The Impact Of Nutrition And Physical Activity Education On The Knowledge, Attitude, And Behavior Of Muslim Youth From Various Ethnic Backgrounds, Hana L. Jaber, Zainab Rida Aug 2016

The Impact Of Nutrition And Physical Activity Education On The Knowledge, Attitude, And Behavior Of Muslim Youth From Various Ethnic Backgrounds, Hana L. Jaber, Zainab Rida

UCARE: Research Products

The Islamic Foundation of Lincoln is a growing population within Lincoln including many different racial backgrounds, ethnic identities, and languages. Due to these various backgrounds communication may impair the education of families as well as their attitudes and behaviors. Although there are programs developed for many other minority groups, the Muslim (population comprising the Islamic Foundation of Lincoln) population does not have any data obtained from the community in terms of nutrition and physical activity behaviors, attitudes, and knowledge. This study seeks to understand if there are barriers to communication and if the intervention enhanced nutrition knowledge. This poster reflects …


Effect Of Taste Stimuli On Swallowing Function In Persons With Traumatic Injuries, Megan Asselin, Angela M. Dietsch Aug 2016

Effect Of Taste Stimuli On Swallowing Function In Persons With Traumatic Injuries, Megan Asselin, Angela M. Dietsch

UCARE: Research Products

Background

Swallowing disorders are prevalent and costly. As of now, there are limited therapeutic options available to treat them. Extant research in limited populations has suggested that swallowing mechanics can be improved by extremely sour liquids, but this has not been tested in traumatically injured populations. However, sour tastants are unpleasant, and more palatable taste mixtures have not been tested.

Methods

The quantitative data were extracted from an existing pool of de-identified video fluoroscopic swallowing studies (moving radiographs) obtained from traumatically injured young adults under another research protocol. Each participant completed swallows of custom-mixed plain, sour, and sweet-sour boluses. Positions …


Whole Genomic Sequence Analysis Of Bacillus Infantis: Defining The Genetic Blueprint Of Strain Nrrl B-14911, An Emerging Cardiopathogenic Microbe, Chandirasegara Massilamany, Akram Mohammed, John Dustin Loy, Tanya Purvis, Bharathi Krishnan, Rakesh H. Basavalingappa, Christy M. Kelley, Chittibabu Guda, Raúl G. Barletta, Etsuko N. Moriyama, Timothy P.L. Smith, Jay Reddy Aug 2016

Whole Genomic Sequence Analysis Of Bacillus Infantis: Defining The Genetic Blueprint Of Strain Nrrl B-14911, An Emerging Cardiopathogenic Microbe, Chandirasegara Massilamany, Akram Mohammed, John Dustin Loy, Tanya Purvis, Bharathi Krishnan, Rakesh H. Basavalingappa, Christy M. Kelley, Chittibabu Guda, Raúl G. Barletta, Etsuko N. Moriyama, Timothy P.L. Smith, Jay Reddy

Jay Reddy Publications

Background: We recently reported the identification of Bacillus sp. NRRL B-14911 that induces heart autoimmunity by generating cardiac-reactive T cells through molecular mimicry. This marine bacterium was originally isolated from the Gulf of Mexico, but no associations with human diseases were reported. Therefore, to characterize its biological and medical significance, we sought to determine and analyze the complete genome sequence of Bacillus sp. NRRL B-14911.

Results: Based on the phylogenetic analysis of 16S ribosomal RNA (rRNA) genes, sequence analysis of the 16S-23S rDNA intergenic transcribed spacers, phenotypic microarray, and matrix-assisted laser desorption ionization time-offlight mass spectrometry, we propose that this …


3-Hydroxy Fatty Acid Induce Trophoblast And Hepatocyte Lipoapoptosis, Taylor Bruett, Taylor Heng, Sathish Kumar Natarajan Aug 2016

3-Hydroxy Fatty Acid Induce Trophoblast And Hepatocyte Lipoapoptosis, Taylor Bruett, Taylor Heng, Sathish Kumar Natarajan

UCARE: Research Products

Acute Fatty Liver of Pregnancy (AFLP) is a very rare and fatal condition for both the mother and the unborn offspring accompanied by severe maternal liver dysfunction. AFLP usually develops during the third trimester of pregnancy with symptoms of liver disease. AFLP is associated with a mutation in the enzyme, long-chain 3-hydroxy acyl CoA dehydrogenase (LCHAD) which is involved in the mitochondrial beta-oxidation of fatty acids. Defective beta-oxidation of fatty acids results in the individual unable to metabolize fatty acids which then causes fatty infiltration in their organs, such as the liver. This interferes with normal function of the liver …


Guidelines For Interpretive Interview Fidelity In Mixed Methods Research Within The Context Of A Randomized Controlled Trial, Amanda L. Garrett Aug 2016

Guidelines For Interpretive Interview Fidelity In Mixed Methods Research Within The Context Of A Randomized Controlled Trial, Amanda L. Garrett

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Interviews fascinate and capture individuals’ attention. Researchers value the data they glean from interviews, while participants enjoy being asked to share their voices and opinions. Some of the most complex, stringent research designs are now being revised to include interviews, such as randomized controlled trials. But, how do we know that the interviews that are conducted are valid? We need to know more about how interviews are developed and delivered within the context of intervention research. Therefore, the aim of this methodological dissertation is to create a set of recommendations for interpretive interviews in a mixed methods randomized controlled trial. …


Icook 4-H Intervention: Food-Related Behavior And Intake Of Adult Main Meal Preparers Participating In A 5-State Childhood Obesity Prevention Study, Kyla A. Richardson Aug 2016

Icook 4-H Intervention: Food-Related Behavior And Intake Of Adult Main Meal Preparers Participating In A 5-State Childhood Obesity Prevention Study, Kyla A. Richardson

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Childhood obesity affects 12.7 million children within the United States. The need for childhood obesity prevention programs is high. Research supports family-centered programs and health interventions rooted within the Social Cognitive Theory (SCT); the iCook 4-H program combines these attributes. The objective of this study was to assess differences in adult outcome variables between control and treatment participants and whether the program impacted food security status.

Participants consisted of adult-youth pairs (dyads) that included a 9-10 year-old child and their adult primary meal preparer. The focus of this project was to provide findings on adult participants only. The program was …


Mucosal Vaccination By Adenoviruses Displaying Reovirus Sigma 1, Eric A. Weaver, Zenaido T. Camacho, Matthew L. Hillestad, Catherine M. Crosby, Mallory A. Turner, Adam J. Guenzel, Hind J. Fadel, George T. Mercier, Michael A. Barry Aug 2016

Mucosal Vaccination By Adenoviruses Displaying Reovirus Sigma 1, Eric A. Weaver, Zenaido T. Camacho, Matthew L. Hillestad, Catherine M. Crosby, Mallory A. Turner, Adam J. Guenzel, Hind J. Fadel, George T. Mercier, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

We previously developed adenovirus serotype 5 (Ad5) vectors displaying the sigma 1 protein from reovirus as mucosal vaccines. Ad5-sigma retargets to JAM-1 and sialic acid, but had 40-fold reduced gene delivery when compared to Ad5. While weaker at transduction, Ad5-sigma generated stronger T cell responses than Ad5 when used for mucosal immunization. New Ad5-fiber-sigma vectors were generated here by varying the number of fiber β-spiral shaft repeats (R) fused between fiber tail and the sigma. Ad5 virions encoding R3, R14, and R20 chimeras were rescued. Increasing chimera length led to their decreasing encapsidation of these proteins in the virions. Ad5-R3 …