Medicine and Health Sciences Commons^™

My Client Knows That He’S About To Stutter: How Can We Address Stuttering Anticipation During Therapy With Young People Who Stutter?, Eric S. Jackson, Hope Gerlach, Naomi H. Rodgers, Patricia M. Zebrowski

Department of Special Education and Communication Disorders: Faculty Publications

Stuttering anticipation is endorsed by many people who stutter as a core aspect of the stuttering experience. Anticipation is primarily a covert phenomenon and people who stutter respond to anticipation in a variety of ways. At the same time as anticipation occurs and develops internally, for many individuals the “knowing” or “feeling” that they are about to stutter is a primary contributor to the chronicity of the disorder. In this article, we offer a roadmap for both understanding the phenomenon of anticipation and its relevance to stuttering development. We introduce the Stuttering Anticipation Scale (SAS)—a 25-item clinical tool that can …

Resilience Concepts In Psychiatry Demonstrated With Bipolar Disorder, David G. Angeler, Craig R. Allen, Maj-Liz Persson

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Background: The term resilience describes stress–response patterns of subjects across scientific disciplines. In ecology, advances have been made to clearly distinguish resilience definitions based on underlying mechanistic assumptions. Engineering resilience (rebound) is used for describing the ability of subjects to recover from adverse conditions (disturbances), and is the rate of recovery. In contrast, the ecological resilience definition considers a systemic change: when complex systems (including humans) respond to disturbances by reorganizing into a new regime (stable state) where structural and functional aspects have fundamentally changed relative to the prior regime. In this context, resilience is an emergent property of complex …

Communicating Nutrition Information At The Point Of Purchase: An Eye‐Tracking Study Of Shoppers At Two Grocery Stores In The United States, Mike Bartels, Katherin Tillack, Chung‐Tung Jordan Lin

United States Food and Drug Administration: Publications

Providing nutrition information at the point of purchase is one approach that can be used to help consumers adopt and maintain a healthy diet. Previous research has examined consumer self‐reported notice and use of the information as well as how the design of the information affects its attention and use in a laboratory environ‐ ment using eye‐tracking methodology. This study advances the literature by applying eye‐tracking methodology to explore consumer visual and choice behavior in a real shopping environment, and by recognizing that nutrition information competes with other visual stimuli in the store and consumers are vulnerable to a “limited …

Complete Genome Sequence Of Moraxella Bovis Strain Epp-63 (300), An Etiologic Agent Of Infectious Bovine Keratoconjunctivitis, John Dustin Loy, Aaron M. Dickey, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

We report here the complete closed genome sequence of Moraxella bovis strain Epp-63 (300) (Epp63). This strain was isolated from an infectious bovine keratoconjunctivitis (IBK) case in 1963. Since then, Epp63 has been used extensively for IBK research. Consequently, the genome sequence of Epp63 should help elucidate IBK host-pathogen interactions.

Non-Naturally Occurring Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) And Methods Of Using, Hiep Lai Xuan Vu, Fernando Osorio, William W. Laegreid, Asit K. Pattnaik, Fangrui Ma

Nebraska Center for Virology: Faculty Publications

A non-naturally occurring porcine reproductive and respiratory syndrome virus (PRRSV) is provided herein, and methods of making and using the non-naturally occurring PRRSV also are provided.

2018 Nebraska Beef Cattle Report (Complete)

Nebraska Beef Cattle Reports

Contents

Cow/Calf: Analyses of Birthdate and Growth in Beef Heifers Categorized by Puberty and Pregnancy Status • Letrozole: A Steroid-Free Estrous Synchronization Method • Comparison of Two Alternate Prostaglandin Products in Yearling Beef Heifers • Impact of Pre-and Postpartum Nutrition on March-calving Cow and Progeny Productivity • Effects of Late Gestation Supplementation, Synchronization, and Creep Feeding in a Spring Calving Beef Herd in the Nebraska Sandhills • Effect of Supplementation during the Breeding Season on a May-calving Herd in the Nebraska Sandhills • Impact of Winter Supplementation of May Calving Cows and Heifer Development System in Two Different Breeding Seasons …

Impact Of Intake On Methane Production In Growing Steers, Tom M. Winders, Bradley M. Boyd, F. Henry Hilscher Hilscher, Samodha C. Fernando, Richard Stowell, Galen E. Erickson

Nebraska Beef Cattle Reports

A study was conducted to evaluate the impact that level of intake has on methane and carbon dioxide production by growing steers. Two treatments were evaluated that included ad-libitum intake compared to limit-fed steers. The ad-libitum fed cattle had greater gains, similar feed efficiency and produced more methane and carbon dioxide per day, while the limit fed cattle produced more methane and carbon dioxide per pound of intake than the ad-libitum fed cattle.

Supplementing Rumen Undegradable Protein To Grazing Cattle, Braden C. Troyer, Bradley M. Boyd, Andrea K. Watson Watson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A pooled-analysis of previous Nebraska Beef Report Articles examined the impact of rumen undegradable protein (RUP) supplementation for cattle grazing different types of forage. Each lb of RUP supplement increased ADG by 0.63 lb/d when cattle were grazing smooth brome and 0.43 lb/d when grazing warm season grasses. Cattle did not respond to RUP when grazing summer annuals which were high (18.2%) in CP.

Experience Improves The Reliability Of Subjective Measurements Of Temperament In Beef Cattle, Jaime T. Praham, Amy E. Tanner, Mark L. Wahlberg Wahlberg, W. S. Swecker Jr., Ronald M. Lewis

Nebraska Beef Cattle Reports

Reliability of experienced and inexperienced observers when assessing the behavior of cattle when restrained in a squeeze chute (chute score), and when exiting the chute (exit score), was compared. Overall, experienced observers had higher reliability than inexperienced observers. Increasing the number of individuals scoring an animal decreased the degree of agreement. However, within an acceptable tolerance for difference in scores, such disagreement may be beneficial; it allows for subtlety in interpretations of temperament, which when averaged, may better reflect docility. Reliabilities were higher for exit score than chute score. This may reflect the complexity of the trait being evaluated, with …

Antimicrobial Interventions And Application Time Effects On Ground Beef Quality, Ashley R. Mccoy, Dennis E. Burson, Gary A. Sullivan Sullivan

Nebraska Beef Cattle Reports

Small business meat processors can use organic acid antimicrobial interventions to control Shiga toxin-producing E. coli (STEC) when producing ground beef; however, many small producers are concerned about the impact on ground beef quality. The effects of two commonly used organic acids, lactic acid and peroxyacetic acid, were evaluated at short (15 seconds) or extended (3 minutes) raw material dip times on ground beef quality parameters. Beef trim dipped in lactic acid for 3 minutes had a reduction in total aerobic bacteria plate count, but also increased ground beef discoloration and lipid oxidation during retail display. Use of a shorter …

Steer Performance Grazing Corn Residue And Supplemented With Modified Distillers Grains Plus Solubles With Or Without Urea, Robert M. Jones, Robby G. Bondurant, F. Henry Hilscher Hilscher, Jim C. Macdonald

Nebraska Beef Cattle Reports

A growing study was conducted to evaluate the effects of supplementing growing calves grazing corn residue with modified distillers grains plus solubles (MDGS; 3 or 5 lb/d) and with or without urea on growth performance. There were no significant MDGS × urea inclusion interactions observed. Urea inclusion level (0 and 0.12 lb/d) did not affect supplemental intake, ADG, or ending BW. Steers fed 5 lb of MDGS had an increased ADG and a heavier ending BW compared to steers fed 3 lb MDGS daily. Supplemental urea is not necessary when supplementing at least 3 lb MDGS to steers grazing corn …

Evaluation Of Corn Distillers Solubles On Growing Steer Performance, Benjamin H. Hansen, Robert G. Bondurant, F. Henry Hilscher Hilscher, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

A growing study evaluated increasing inclusions of corn distillers solubles (CDS) at 10, 20, 30, and 40%, or increasing wet distillers grains plus solubles (WDGS) at 10, 20, 30, and 40% compared to a corn control. Corn was replaced by CDS or WDGS in each forage-based diet. Increasing CDS resulted in a quadratic increase for both DMI and F:G. Increasing WDGS linearly increased both DMI and ADG with no effect on F:G. The energy value of CDS is less than that of corn, whereas WDGS had an energy value similar to corn in growing diets with 50% brome hay. A …

Analyses Of Birthdate And Growth In Beef Heifers Categorized By Puberty And Pregnancy Status, Aline Gomes Da Silva, Andy Roberts, T. L. Meyers, Richard N. Funston

Nebraska Beef Cattle Reports

Heifer records were retrospectively evaluated to see if Julian birthdate, cycling status prior to breeding, and body weight collected from weaning through final pregnancy diagnosis differed when heifers were categorized by 5 different approaches: 1) pubertal status prior to estrous synchronization, 2) whether or not detected in estrus at AI, 3) heifers impregnated by AI vs all other heifers, 4) final pregnancy status, and 5) a 5-way classification accounting for AI and pregnancy status (AI pregnant, heifers subjected to AI that subsequently conceived to bull, heifers not AI that were impregnated by bull, heifers subjected to AI that were not …

The Effect Of Harvest Method And Ammoniation Of Corn Residue On Growing Calf Performance, Ashley C. Conway, Robert G. Bondurant, F. Henry Hilscher Hilscher, James C. Macdonald, Terry J. Klopfenstein, Mary Drewnoski

Nebraska Beef Cattle Reports

A growing trial was conducted to determine the effect of feeding baled corn residue harvested using three different methods, with and without ammoniation of the residue. Residue harvested with the New Holland Cornrower™ with two rows of chopped stem added to the windrow resulted in a 9% increase in ADG compared to conventional rake and bale harvest or turning off the combine spreader and baling tailing. Ammoniation of residue increased ADG 67% (increase of 1.1 lb/d) over non-ammoniated residue. Feed efficiency was not affected by harvest method, but ammoniation decreased F:G by 13% compared to cattle fed non-ammoniated residue. Although …

Impact Of Corn Oil Removal From Modified Distillers Grains Plus Solubles And Supplemental Corn Oil On Finishing Cattle Performance, Jordan E. Burhoop, Curtis J. Bittner, F. Henry Hilscher Hilscher, Matt K. Luebbe, Jim C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study was conducted to evaluate removal of corn oil from modified distillers grains plus solubles (MDGS) and replacement of supplemental corn oil on finishing cattle performance. Four treatments were evaluated: a corn control diet, 40% de-oiled MDGS, 38% de-oiled MDGS plus 2% corn oil to equal the fat content of full fat MDGS, or 40% full fat MDGS. There was a significant improvement in ADG and F:G for cattle fed de-oiled MDGS plus oil compared to other treatments. Cattle fed full fat MDGS had numerically lower ADG and numerically poorer F:G (3.7%) compared with cattle fed MDGS plus …

Intensive Referral To Mutual-Help Groups: A Field Trial Of Adaptations For Rural Veterans, Kathleen M. Grant, Lance Brendan Young, Kimberly A. Tyler, Jamie L. Simpson, R. Dario Pulido, Christine Timko

Department of Sociology: Faculty Publications

Objective: A multisite field trial testing whether improved outcomes associated with intensive referral to mutual help groups (MHGs) could be maintained after the intervention was adapted for the circumstances and needs of rural veterans in treatment for substance use disorder (SUD). Methods: In three Veterans Affairs treatment programs in the Midwest, patients (N = 195) received standard referral (SR) or rural-adapted intensive referral (RAIR) and were measured at baseline and 6-month follow-up. Results: Both groups reported significant improvement at 6 months but no significant differences between SR and RAIR groups in MHG participation, substance use, addiction severity, and posttraumatic stress …

Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford

United States Department of Agriculture Wildlife Services: Staff Publications

Anticoagulant rodenticides (ARs) are used to control rodents around homes, buildings, and in agriculture. They have been found widely in predatory and scavenging wildlife as a result of secondary exposure and less commonly in herbivores and omnivores from primary exposure. While predators and scavengers have been monitored for AR exposure, very little information is available about AR residues in edible muscle tissue of game animals. Game animals may be exposed to ARs through direct consumption of bait, ingestion of contaminated food or vegetation, or consumption of contaminated prey items. Carcasses of three species of game animals (black bear, wild pigs, …

Understanding Parental Ethnotheories And Practices About Healthy Eating: Exploring The Developmental Niche Of Preschoolers, Deepa Srivastava, Julia C. Torquati, Maria Rosario De Guzman, Dipti A. Dev

Department of Child, Youth, and Family Studies: Faculty Publications

Purpose: To understand parental ethnotheories (i.e., belief systems) and practices about preschoolers’ healthy eating guided by the developmental niche framework.

Design: Qualitative hermeneutic phenomenology.

Setting: Home.

Participants: Participants were 20 parents of preschool-age children ages 3 to 5 years, recruited from a quantitative investigation. A majority of the participants were white, female, married, well educated, and working full time.

Methods: Participants who completed the quantitative survey were asked to provide their contact information if they were willing to be interviewed. From the pool of participants who expressed their willingness to participate in the interviews, 20 participants were selected …

The Rhetoric Of Science Education And Technology, Iwasan D. Kejawa

School of Computing: Faculty Publications

Nearly thousands of science experiments are performed both on humans and animals every year in the United States (Gregory, 1999). Does Science enormously play a role in the well-beings of individual in the society? Research has found that science education is through motivation and satisfying the needs of humans. The scientific world is part of an elongated human development. This can be substantiated with the use and evolution of TECHNOLOGY and SCIENCE (Minton, 2004). Education of the entities that comprise the need to achieve the goal of TECHNOLOGY and SCIENCE which are important issues of today. Research has shown that …

Community Health Worker Employer Survey: Perspectives On Chw Workforce Development In The Midwest, Virginia Chaidez, Angela L. Palmer-Wackerly, Kate Trout

Department of Communication Studies: Faculty Publications

A statewide Community Health Worker Employer Survey was administered to various clinical, community, and faith-based organizations (n = 240) across a range of rural and urban settings in the Midwest. At least 80% of participants agreed or strongly agreed that items characterized as supervisory support were present in their work environment. Thirty-six percent of respondents currently employed CHWs, over half (51%) of survey respondents reported seeing the need to hire/work with more CHWs, and 44% saw the need for CHWs increasing in the future. Regarding CHW support, a majority of respondents indicated networking opportunities (63%), paid time for networking (80%), …

Metatranscriptome Of Human Faecal Microbial Communities In A Cohort Of Adult Men, Galeb S. Abu-Ali, Raaj S. Mehta, Jason Lloyd-Price, Himel Mallick, Tobyn Branck, Kerry L. Ivey, David A. Drew, Casey Dulong, Eric Rimm, Jacques Izard, Andrew T. Chan, Curtis Huttenhower

Department of Food Science and Technology: Faculty Publications

The gut microbiome is intimately related to human health, but it is not yet known which functional activities are driven by specific microorganisms' ecological configurations or transcription. We report a large-scale investigation of 372 human fecal metatranscriptomes and 929 metagenomes from a subset of 308 men in the Health Professionals Follow-Up Study. We identified a metatranscriptomic 'core' universally transcribed over time and across participants, often by different microorganisms. In contrast to the housekeeping functions enriched in this core, a 'variable' metatranscriptome included specialized pathways that were differentially expressed both across participants and among microorganisms. Finally, longitudinal metagenomic profiles allowed ecological …

Brain Is A Potential Sanctuary For Subtype C Hiv-1 Irrespective Of Art Treatment Outcome, For Yue Tso, Guobin Kang, Eun Hee Kwon, Peter Julius, Qingsheng Li, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Subtype C HIV-1 is responsible for the largest proportion of people living with HIV-1 infection. However, there is limited information about the roles of the brain and its cell types as a potential sanctuary for this subtype and how the sanctuary may be affected by the administration of anti-retroviral therapy (ART). To address this issue, we collected postmortem brain tissues from ART treated HIV-1 infected Zambian individuals who experienced complete viral suppression and those who did not. Tissues from various brain compartments were collected from each individual as frozen and formalin-fixed paraffin embedded brain specimens, for detection and quantification of …

Brain Is A Potential Sanctuary For Subtype C Hiv-1 Irrespective Of Art Treatment Outcome, For Yue Tso, Guobin Kang, Peter Julius, Qingsheng Li, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Subtype C HIV-1 is responsible for the largest proportion of people living with HIV-1 infection. However, there is limited information about the roles of the brain and its cell types as a potential sanctuary for this subtype and how the sanctuary may be affected by the administration of anti-retroviral therapy (ART). To address this issue, we collected postmortem brain tissues from ART treated HIV-1 infected Zambian individuals who experienced complete viral suppression and those who did not. Tissues from various brain compartments were collected from each individual as frozen and formalin-fixed paraffin embedded brain specimens, for detection and quantification of …

Contribution Of The Gp120 V3 Loop To Envelope Glycoprotein Trimer Stability In Primate Immunodeficiency Viruses, Dane Bowder, Haley Hollingsead, Kate Durst, Duoyi Hu, Wenzhong Wei, Joshua Wiggins, Halima Medjahed, Andrés Finzi, Joseph Sodroski, Shi-Hua Xiang

Nebraska Center for Virology: Faculty Publications

The V3 loop of the human immunodeficiency virus type 1 (HIV-1) gp120 exterior envelope glycoprotein (Env) becomes exposed after CD4 binding and contacts the coreceptor to mediate viral entry. Prior to CD4 engagement, a hydrophobic patch located at the tip of the V3 loop stabilizes the non-covalent association of gp120 with the Env trimer of HIV-1 subtype B strains. Here, we show that this conserved hydrophobic patch (amino acid residues 307, 309 and 317) contributes to gp120-trimer association in HIV-1 subtype C, HIV-2 and SIV. Changes that reduced the hydrophobicity of these V3 residues resulted in increased gp120 shedding and …

Ultrastructure Of Meelsvirus: A Nuclear Virus Of Arrow Worms (Phylum Chaetognatha) Producing Giant "Tailed" Virions, George L. Shinn, Brianna L. Bullard

Nebraska Center for Virology: Faculty Publications

Most known giant viruses, i.e., viruses producing giant virions, parasitize amoebae and other unicellular eukaryotes. Although they vary in the level of dependence on host nuclear functions, their virions self-assemble in the host cell's cytoplasm. Here we report the discovery of a new prototype of giant virus infecting epidermal cells of the marine arrow worm Adhesisagitta hispida. Its 1.25 μm-long virions self-assemble and accumulate in the host cell's nucleus. Conventional transmission electron microscopy reveals that the virions have a unique bipartite structure. An ovoid nucleocapsid, situated in a broad "head" end of the virion is surrounded by a thin …

Development Of Septic Polysynovitis And Uveitis In Foals Experimentally Infected With Rhodococcus Equi, Laura Huber, Steeve Giguère, Londa J. Berghaus, Amanda Hanafi, Sarah Vitosh-Sillman, Sarah L. Czerwinski

School of Veterinary and Biomedical Sciences: Faculty Publications

Rhodococcus equi is one of the most important causes of disease in foals. Infection is typically characterized by pyogranulomatous pneumonia although extrapulmonary infections occur occasionally. Uveitis and polysynovitis have been reported in foals naturally infected with R. equi and are thought to be the result of an immune-mediated process. However, the pathogenesis of these conditions is poorly understood. The objectives of this study were to document the occurrence of uveitis and polysynovitis after experimental infection with R. equi and to determine if these disorders are the direct result of infection at these sites. Foals between 3 and 4 weeks of …

Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt

School of Veterinary and Biomedical Sciences: Faculty Publications

Hydrated lime manure treatment was evaluated to determine porcine epidemic diarrhea virus (PEDV) susceptibility to alkaline stabilization. At pH 10, PEDV decreased (quantitative polymerase chain reaction) and lost infectivity (swine bioassay). Although ammonium decreased above pH 9 (up to 25%), alkaline stabilization managed to control potential infection from manure sources.

Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry Identification Of Moraxella Bovoculi And Moraxella Bovis Isolates From Cattle, Kara Robbins, Aaron M. Dickey, Michael L. Clawson, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Infectious bovine keratoconjunctivitis (IBK) is an economically significant disease caused by Moraxella bovis. Moraxella bovoculi, although not reported to cause IBK, has been isolated from the eyes of cattle diagnosed with IBK. Identification of M. bovis and M. bovoculi can be performed using biochemical or DNA-based approaches, both of which may be time consuming and inconsistent between laboratories. We conducted a comparative evaluation of M. bovoculi and M. bovis identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a database provided by Bruker Daltonics (termed the BDAL database), the BDAL database supplemented with spectra generated in …

Circadian Behavioral Responses To Light And Optic Chiasm-Evoked Glutamatergic Epscs In The Suprachiasmatic Nucleus Of Iprgc Conditional Vglut2 Knock-Out Mice, Michael G. Moldavan, Patricia J. Sollars, Michael R. Lasarev, Charles N. Allen, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

Intrinsically photosensitive retinal ganglion cells (ipRGCs) innervate the hypothalamic suprachiasmatic nucleus (SCN), a circadian oscillator that functions as a biological clock. ipRGCs use vesicular glutamate transporter 2 (vGlut2) to package glutamate into synaptic vesicles and light-evoked resetting of the SCN circadian clock is widely attributed to ipRGC glutamatergic neurotransmission. Pituitary adenylate cyclase-activating polypeptide (PACAP) is also packaged into vesicles in ipRGCs and PACAP may be coreleased with glutamate in the SCN. vGlut2 has been conditionally deleted in ipRGCs in mice [conditional knock-outs (cKOs)] and their aberrant photoentrainment and residual attenuated light responses have been ascribed to ipRGC PACAP release. However, …

Development Of A Multiplex Real-Time Pcr Assay Using Two Thermocycling Platforms For Detection Of Major Bacterial Pathogens Associated With Bovine Respiratory Disease Complex From Clinical Samples, John Dustin Loy, Laura Leger, Aspen M. Workman, Michael L. Clawson, Ece Bulut, Bing Wang

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine respiratory disease complex (BRDC) is one of the most significant diseases of cattle. Bacterial pathogens involved in BRDC include Mannheimia haemolytica, Mycoplasma bovis, Histophilus somni, and Pasteurella multocida. We developed and evaluated a multiplexed real-time hydrolysis probe (rtPCR) assay using block-based Peltier and rotary-based thermocycling on lung tissue, nasal swabs, and deep nasopharyngeal swabs. The rtPCR results were compared to culture or a gel-based M. bovis PCR using statistical analysis to determine optimum quantification cycle (Cq) cutoffs to maximize agreement. The limits of detection were 1.2–12 CFU/reaction for each pathogen. M. haemolytica was the most prevalent organism detected …