Medicine and Health Sciences Commons^™

Effects Of L-Fucose Supplementation On The Viability Of Cancer Cell Lines, Muhammad Alif Mazlan, Afzan Mat Yusof, Muhammad Lokman Md Isa

Makara Journal of Health Research

Background: Fucose is a deoxyhexose sugar. While the biological roles of L-fucose remain unclear, the sugar is known to accelerate the malignant potential of cancer cells. Therefore, this study aimed to evaluate the viability pattern of human cancer and normal cell lines treated with fucose.

Methods: The human gingival fibroblast (HGF-1), colorectal adenocarcinoma (HT-29) and skin malignant melanoma (A375) cell lines were cultured and treated with fucose at three concentrations of 1, 5, and 10 mg/ml. Cell viability was then measured using (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using Statistical Package for the Social …

Cd34+ Ucb Stem Cells Attenuate Tgf-Β Signaling And Inhibit Liver Fibrosis: A New Avenue For Liver Cirrhosis-Carcinogenesis Prevention, Wahyunia Likhayati Septiana, Radiana Dhewayani Antarianto, Melva Louisa, Ahmad Aulia Jusuf, Atikah Chalida Barasila, Jeanne Adiwinata Pawitan, Iqbal Fasha

Makara Journal of Health Research

Background: The liver microenvironment plays a key role in liver fibrosis and carcinogenesis. This study aimed to fill the gap in knowledge on the interaction between hepatic stellate cells and endothelial progenitor cells with biomarkers of liver fibrosis and/or carcinogenesis, including Col1A1, TGF-β, and tenascin-C.

Methods: CD34⁺ stem cells were isolated from umbilical-cord-blood mononuclear cells. 2D and 3D co-culture of CD34⁺ UCB SCs and LX2 was performed. The cells were incubated in a CO₂ incubator for three days. Morphological observation, qRT-PCR of TGF-β1 and COL1A1, and immunocytochemistry of tenascin-C were performed.

Results: CD34^{+ …}

Serum Immune Profiling Of Patients With Polycystic Ovary Syndrome, Hifsa Mobeen, Muhammad Kashif, Ahmed Nadeem, Faheem Shahzad, Romeeza Tahir, Afia Abbas, Nadeem Afzal

Makara Journal of Health Research

Background: Polycystic ovary syndrome (PCOS), the most prevalent hormonal disorder in females, is characterized by low levels of progesterone, which causes increased estrogen levels leading to production of various anti and auto-antibodies. This study aimed to estimate and compare levels of anti-nuclear antibodies (ANA), anti-thyroid peroxidase (anti-TPO), anti-thyroglobulin (anti-TG), and anti-islet cell antibodies (anti-ICAb) in patients with PCOS and healthy controls.

Methods: The present comparative study included 82 subjects divided into two groups with 41 individuals in each group. Group I included healthy subjects, while Group II included patients diagnosed with PCOS. Blood samples were collected to determine …

Health-Related Quality Of Life And Work Ability Of Smallholder Rice Farm Workers In San Jose, Occidental Mindoro, Philippines, Artemio Morado Gonzales Jr, Ryan Mark Aloro Ambong, Leoniel Salibio Bais, Lendon Perez Macaspac

Makara Journal of Health Research

Background: Health is an integral form of human capital that can positively influence agricultural worker productivity in the physical, mental, and social domains. Poor health usually represents a burden to farm workers because a failure to meet scheduled tasks on the farm can later affect the dependents who rely on it for food nourishment and sustained livelihood. This study aims to determine the association between health and the work capabilities of smallholder rice farm workers in San Jose, Occidental Mindoro, Philippines.

Methods: A cross sectional study was conducted among 100 farm workers. The SF-36 (HRQoL) and Work Ability …

Perceived Need And Utilization Of Dental Health Care Services In Indonesia: A Secondary Analysis Using The National Socioeconomic Data, Normaliza Ab Malik, Robbykha Rosalien, Nadine Khalissya, Iwany Amalliah Badruddin, Diah Ayu Maharani

Makara Journal of Health Research

Background: Perceived needs and utilization of oral health services are important to ensure improvements in oral health outcomes and to allocate resources for the continuous provision of the services. This study aimed to determine the factors influencing the perceived needs and utilization of oral health services in Indonesia.

Methods: Secondary data from the 2013 Indonesian National Socioeconomic Survey (n = 260,925) was used. Descriptive analysis and logistic regression were employed to describe the relationship between perceived needs and utilization of oral health services.

Results: The proportions of Indonesians who had a perceived need for oral health services and …

Indonesian Health Professions Students’ Perceptions Toward An Interprofessional Education Program: Findings After Five Years Of Implementation, Santi Purna Sari, Diantha Soemantri, Dian Ayubi, Evi Martha, Hanny Handiyani, Ardi Findyartini

Makara Journal of Health Research

Background: The Interprofessional Education (IPE) program is important for preparing health professions students to provide future interprofessional collaborative practice. The Universitas Indonesia Health Sciences Cluster has been implementing the IPE program since 2013. A comprehensive evaluation is required following the implementation of the IPE program. The aim of the study was to evaluate the IPE course based on perceptions of undergraduate students at the Universitas Indonesia from 2013 to 2017.

Methods: A mixed-methods study utilizing a semi-structured questionnaire was conducted with first year students following the completion of the first stage of the IPE course. A total of …

Organizational Empowerment Among Nursing Faculty In The Northwestern Region Of Saudi Arabia, Salman Hamdan Alsaqri, Eddieson Pasay-An, Liza Villacorte, Fredezwinda Madjid, Carmencita Pacis, Ahmad Khalil Ahmad Al-Sadi

Makara Journal of Health Research

Background: This study aimed to determine the differences in the workplace empowerment of nursing faculty as it relates to their demographic information.

Methods: A comparative cross-sectional study was conducted among 65 nurse educators at the College of Nursing, University of Hail, Saudi Arabia. The data were collected through a survey questionnaire between January and March 2019.

Results: Significant differences were found regarding gender and opportunity (p < 0.017), support (p < 0.020), resources (p < 0.022), job activities scale (JAS; p < 0.005), organizational relationship scale (ORS; p < 0.011), and marital status on resources (p < 0.031). In comparison, gender to information and all of the other demographic variables such as marital status (except resources), years of experience, age, nationality, educational qualifications, and specialization were found insignificant to opportunity, support, resources, information, JAS, and ORS, where all of their p-values were more than 0.05.

Conclusion: Male nursing faculty were found to be more empowered in terms of opportunity, support, resources, JAS, and …

Effect Of The Sims Program On Oral Hygiene Levels Of 5–6-Year-Old Children In The Kampar District, Malaysia: A Cluster-Randomized Controlled Trial, Nurul Hayati Anwar, Nor Azlida Mohd Nor, Zamros Yuzadi Mohd Yusof

Makara Journal of Health Research

Background: This study evaluated the effect of the “Senyuman Indah Milik Semua” program (SIMSP) versus the existing preschool oral healthcare program (POHP) on children’s oral health and parents’ oral health literacy (OHL) in Kampar district, Malaysia.

Methods: This was a cluster-randomized, matched pair, examiner-blind, controlled trial. Using computer-generated random tables, 14 preschools were allocated to the SIMSP intervention over 6 months and another 14 were allocated to the POHP, which was concealed at the cluster level. Healthy 5–6-year-old children and parents who understood the Malay language were recruited. The SIMSP was comprised of preschool visits by dental …

Cytokine Storm In Covid-19: An Overview, Mechanism, Treatment Strategies, And Stem Cell Therapy Perspective, Nuriza Ulul Azmi, Meidi Utami Puteri, Donny Lukmanto

Pharmaceutical Sciences and Research

Coronavirus disease 2019 (COVID-19) is the disease caused by the highly transmittable novel coronavirus (SARS-CoV-2) infections. The disease was found at the end of December 2019, in Wuhan, China, and quickly spread worldwide with a higher mortality rate compared to the previous coronavirus disease. Cytokine plays an important role in the inflammatory response against coronavirus infection. However, in severely ill patients with COVID-19, an excessive amount of cytokine serum level (cytokine storm) has been associated with the aggravation of coronavirus disease leading to acute respiratory distress syndrome (ARDS). Thus, the management of the cytokine storm could be the key to …

A Mini Review : Clinically Significant Potential Drug-Drug Interactions In Covid-19 And Comorbid Therapy, Ana Khusnul Faizah, Nani Wijayanti Dyah Nurrahman, Oki Nugraha Putra

Pharmaceutical Sciences and Research

Coronavirus disease 2019 (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and can be aggravated by comorbid diseases. In administering COVID-19 therapy, we need to consider potential drug-drug interactions (pDDIs) with comorbid drugs. Most patients with comorbid diseases get polypharmacy, therefore the risk of pDDIs increases. Potential drug-drug interactions can cause unwanted effects such as toxicity to death. There is no on-label therapy for COVID-19 but FDA has Emergency Use Authorization (EUA) for hydroxychloroquine, chloroquine, azithromycin, remdesivir, ritonavir, and lopinavir. Some COVID-19 treatment potential drug-drug interactions have a level of severity C and D, so …

Chloroquine: An Old To Be Repurposed Drug For Covid-19 Infection (Risk And Benefit), Oki Nugraha Putra, Ana Khusnul Faizah, Nani Wijayanti Dyah Nurrahman, Hardiyono Hardiyono

Pharmaceutical Sciences and Research

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus Disease 2019 (COVID-19) with a high rate of mortality. Chloroquine and hydroxychloroquine have been used for antimalarial and autoimmune diseases for many years. Due to low toxicity and well tolerability as well as immunomodulatory properties, these drugs are proposed to treat viral infection. Some studies, both in vitro and in a clinical setting, have been evaluated for their ability to treat SARS- CoV-2 as promising therapies. Although The National Agency of Drugs and Food Control of The Republic of Indonesia issued emergency authorization for chloroquine and hydroxychloroquine to be used against …

Dermaseptin-Based Antiviral Peptides To Prevent Covid-19 Through In Silico Molecular Docking Studies Against Sars-Cov-2 Spike Protein, Taufik Muhammad Fakih

Pharmaceutical Sciences and Research

A pandemic coronavirus disease of 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has now been declared a global pandemic by the World Health Organization. The search for new drugs, especially by utilizing antiviral peptides is a very potential area. Through this study, protein-peptide docking and protein-protein docking simulations were conducted using in silico methods to identify, evaluate, and explore the molecular affinity and interaction of dermaseptin peptide molecules produced by frogs of the genus Phyllomedusa against the SARS-CoV-2 spike protein macromolecule, and its effect on attachment to the surface of the ACE-2 (Angiotensin Converting Enzyme-2) receptor. …

Remdesivir: Mechanism And Effectiveness For Coronavirus Disease 2019 (Covid-19), Vina Neldi, Suharjono Suharjono

Pharmaceutical Sciences and Research

Currently, coronavirus disease 2019 (COVID-19) is a very serious health problem. The World Health Organization (WHO) has characterized this disease as a pandemic. Recommendations related to vaccines and drugs are not available yet because they are still in the clinical trial phase, and one of the superior drugs is remdesivir which has an antiviral activity. Several clinical trials of this drug are being carried out with the aim of evaluating its safety and efficacy in COVID-19 patients. There are two clinical trials with completed recruitment status, which are NCT04257656 and NCT04280705. NCT04257656 showed that remdesivir had faster time for clinical …

The Potential Roles Of Jamu For Covid-19: A Learn From The Traditional Chinese Medicine, Dwi Hartanti, Binar Asrining Dhiani, Shintia Lintang Charisma, Retno Wahyuningrum

Pharmaceutical Sciences and Research

As the pandemic of the coronavirus disease 2019 (COVID-19) continues while there is no drug and vaccine available, every effort to discover one should be considered. This review aimed to discuss the potential use of jamu, the Indonesian traditional herbal medicine, to deal with COVID-19 by following those of more-established traditional Chinese medicine (TCM). The online literature search using the PubMed database, as well as the circulars from the Indonesian Ministry of Health, were carried out to collect data up to June 07, 2020. The use of TCM for the treatment and prevention of COVID-19 has been officiated in the …

Prediction Of Asiatic Acid Derivatives Affinity Against Sars-Cov-2 Main Protease Using Molecular Docking, Ida Musfiroh, Alia Resti Azura, Driyanti Rahayu

Pharmaceutical Sciences and Research

COVID-19 is a pandemic that currently occurs in almost all parts of the world, caused by a new coronavirus species that can infect humans, namely SARS-CoV-2. To date, there is no effective drug to treat COVID-19. There are studies proving that the secondary metabolites of pentacyclic triterpenes have antiviral activity, one of which is asiatic acid. The aims of this study are to obtain the affinity and interactions of asiatic acid derivative structures in inhibiting the main protease of SARS-CoV-2. The research method was molecular docking of asiatic acid and its derivatives against the main protease of SARS-CoV-2 (6LU7) consisting …

Sars-Cov-2: Virology And Drug Repurposing Approaches, Ratika Rahmasari, Heri Setiawan, Rezi Riadhi Syahdi, Ayun Arifianti, Marina Ika Irianti, Rani Sauriasari, Juliann Nzembi Makau, Muhareva Raekiansyah

Pharmaceutical Sciences and Research

An emerging coronavirus, SARS-CoV-2, is the causative agent for the ongoing pandemic of coronavirus disease 2019 (COVID-19), which has caused a worldwide social and economic disruption. Currently, no antiviral drugs with proven clinical efficacy, or vaccines for its prevention. Therefore, to combat the pandemic of this novel coronavirus, new effective treatments are urgently needed. In the process of traditional drug development, developing new drugs from scratch is a time- consuming process, requires high-investment, and is a high-risk process, which is impractical to face the immediate global challenge of the SARS-CoV-2 pandemic. Drug repurposing strategy is one of the effective ways …

Effective Ultrasound And Neural Mobilization Combinations In Reducing Hand Disabilities In Carpal Tunnel Syndrome Patients, Ni Luh Nopi Andayani, Ari Wibawa, Made Hendra Satria Nugraha

Jurnal Keperawatan Indonesia

Excessive activity in the hands and wrists over a prolonged period of time can cause repetitive strain injury, which leads to the occurrence of carpal tunnel syndrome. The purpose of this study is to determine the differences in the effectiveness of ultrasound therapy and neural mobilization interventions with ultrasound therapy and passive stretching in reducing hand disabilities in patients with carpal tunnel syndrome. It is an experimental study, using the pre-and post-test control group design. The sampling technique employed was simple random sampling, with a study sample comprising 30 people.The difference test with an independent t-test showed a significant difference …

Pre-Testing Of The Who’S Educational Video: “How To Protect Yourself Against Covid-19”, Hilyatul Fadliyah, Manendra Muhtar, Munih Munih, Sylviasari Risgiantini, Weny Wulandary, Hadi Pratomo

Kesmas

The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was declared a public health emergency in many countries, including Indonesia. The World Health Organization (WHO) released an educational video, entitled "How to Protect Yourself Against COVID-19", which explained how to prevent the spread of COVID-19. This study is a qualitative study that aims to pre-test this video, based on the Information, Education, Communication (IEC) theory, by analyzing teenagers’ perceptions of the video. The design used was a Rapid Assessment Procedure (RAP), and the data were collected by conducting in-depth interviews with participants, through …

Impact Of Lockdown In India: A Case Study Comparing Kanataka With International Model, Stelvin Sebastian, Aby Paul, Jeeva Joseph, Joel Joby, Sanjo Saijan, Jobin Kunjumon Vilapurathu

Kesmas

The COVID-19 was declared a pandemic and a global health emergency by WHO, prompting various countries to implement early and stringent social distancing protocols through lockdown, to flatten the epidemic curve. The objective of the present study was to assess the impacts and effectiveness of the lockdown protocol in Karnataka and Punjab, compared with the implementation of this method in Australia and the UK. This study involved the collection of data from different authorized databases, in two phases. The first phase included the time starting with the first-reported index case through the 14th day after the declaration of lockdown, for …

Review Of Trial Therapies And Treatment For Covid-19: Lessons For Indonesia, Halma Zahro Mukhlida, Hilma Hasro Maulida, Gunanti Khairunnisa, Margaretha Josephine Mantrono, Risky Kusuma Hartono, Rindu Rindu, Eka R W Purnamasari, Rizky Fajar Meirawan

Kesmas

The infectious disease from Coronavirus Disease 2019, or COVID-19, has quickly spread world-wide since 2019. Therapies for managing COVID-19 have yet to be confirmed as medication for the severe sickness that the disease may cause. This study aimed to review the previous research of the efficacy of trial therapy and treatment to the patients in the hospital with COVID-19. Using PRISMA guidelines as a method for conducting a systematic literature review, a total of 67 articles were collected from several online journal databases. Various therapies were found that are effective in the treatment and management of COVID-19. In accordance with …

Working Beyond 80: The Meaning Of Work To An Octogenarian In The Workforce, Ma. Mayla Imelda M. Lapa, Laurence L. Gracia, Endrex P. Nemenzo

Jurnal Keperawatan Indonesia

There is an increasing share of people aged 50 years and over in the labor market structure and the rapid aging of the global workforce that supports the latter claim but with a little tank of information on qualitative research describing the experiences of a working octogenarian. An octogenarian is a person who is between 80 and 89 years old. This study aimed to investigate the experiences of a working octogenarian in her fieldwork through a qualitative case study analysis. From the interview, the following three themes were revealed: (a) Work as a legacy, (b) Work as an advocacy, and …

Empowering The Lowland Indigenous Community Through Child Health And Nutrition Program In Occidental Mindoro Province, Philippines, Artemio Morado Gonzales Jr

ASEAN Journal of Community Engagement

Nutrition and child health programs were integrated into the extension programs from 2016-2018 of the Occidental Mindoro State College, in partnership with the local government unit at the municipal and barangay levels. This paper presents the health extension tools and techniques used in preventative care and the promotion of child health services in the indigenous community of Sitio Bato Singit, in the Occidental Mindoro Province, Philippines. Health education, small group techniques, demonstrations, storytelling, informal talks, and open forums were the methods and techniques employed to implement the program. The common tools used were seating arrangements, flip-charting, props, social hours, and …

Analysis Of Knowledge Regarding Biliary Atresia Among Healthcare Providers And Laypersons In East Jakarta After Educational Intervention, Hanna Lianti, Novi Kurnia, Kshetra Rinaldhy, Amanda Safira Aji, M Febriadi Ismet, Rizky Amaliah

ASEAN Journal of Community Engagement

Biliary atresia (BA) is the most common cause of death due to liver disease in children and is the most common indication for pediatric liver transplantation. The success rate of the management of BA is very dependent on the age of the patient at the time of surgery. However, patients with BA were often diagnosed late because of the lack of parents’ and primary healthcare providers’ knowledge regarding BA. This study aims to evaluate the effectiveness of health education in improving the knowledge of healthcare providers and laypersons. A total of 371 respondents participated in this pre-post study with no …

Adaptation To Sexual Dysfunction In Patients With Chronic Renal Failure, Akhyarul Anam, Lestari Sukmarini, Agung Waluyo

Jurnal Keperawatan Indonesia

The prevalence of chronic renal failure in Indonesia tends to increase in the lower age group (45–54 years).Chronic renal failure may lead to impaired sexual function. A descriptive phenomenology study with in-depth interviews was carried out with 12 participants, and thematic content analysis was applied. Six themes were revealed, as follows: 1) adaptation process to sexual dysfunction experienced, 2) sexual dysfunction experience,3)importance of fulfilling sexuality needs,4) behavior in dealing with sexual dysfunction, 5) perception of the cause of sexual dysfunction, and 6) participants’ expectation of health service related to sexual function. The experience of adapting to sexual dysfunction became a …

Job Demands–Resources Model Affects The Performance Of Associate Nurses In Hospital, Khairunnisa Batubara, Bustami Syam, Sri Eka Wahyuni

Jurnal Keperawatan Indonesia

Low nurses’ performance is related with increased job demands and unprofessional job resources. This cross-sectional study aimed to analyze the effects of the job demands–resources model on the performance of associate nurses. The study population was composed of 126 nurses randomly selected. Data were analyzed using the multiple linear regression test. The results showed that job demands and job resources significantly affected the performance of associate nurses. A moderate or heavy level of job demands supported with good job resources will have a positive effect on nurse motivation; thus, nurse’s performance remains good. Job demands must be balanced with job …

Tuberculosis Cases Finding Practice: The Intention Of Cadres, Agnes Pude Lepuen, Cicilia Nony Ayuningsih Bratajaya, Sada Rasmada

Jurnal Keperawatan Indonesia

Tuberculosis (TB) is a difficult health problem to overcome. Active case finding is an important step in managing this infectious disease. However, the prevalence of TB case finding among cadres at the community level is low because of the stigma attached to TB, difficulty in geographical coverage, low public awareness, and social economic barriers. In addition, the empowerment and intention of cadres to perform community-based TB case finding are not optimal yet. This cross-sectional study aimed to determine the intention of TB case finding among 162 public health cadres at one district. Convenient sampling technique was employed in this study. …

Improvement In Patients’ Ability To Care For Anxiety And Impaired Body Image: A Case Report Of Acceptance And Commitment Therapy And Family Psychoeducation, Rasmawati Rasmawati, Budi Anna Keliat, Herni Susanti

Jurnal Keperawatan Indonesia

Heart failure and hypertension are non-communicable diseases that are responsible for 70% of deaths world wide and cause anxiety and impaired body image. Nursing interventions(therapy in general) and acceptance and commitment therapy increase patients’ acceptance of the disease and commitment to alleviate anxiety and improve impaired body image. Meanwhile, family psychoeducation improves the family’s ability to care for the patient. This case report presents two patients with heart failure and hypertension. The two patients experienced a decrease in symptoms on the cognitive aspects(difficulty concentrating, focusing on self, and decline body changes), affective aspects (worry, shame, and despair), physiological aspects(sleep disorders …

Nurses’ Awareness On Patient Safety Culture In A Newly Established University Hospital, Mohd Said Nurumal, Najwa Madinah Sabran, Siti Hazariah Abdul Hamid, Muhammad Kamil Che Hasan

Jurnal Keperawatan Indonesia

As a vital part of patient care delivery, patient safety culture contributes to the quality of care provided by nurses. Safe patient care is positively linked to the attitudes of nurses. This study aimed to assess the perception of nurses working in a newly established teaching hospital. A cross-sectional study involving 194 nurses from three different units was conducted by using a 24-item Hospital Survey of Patient Safety Culture. Data on gender, working unit, age, years of working, and attendance in workshops on patient safety were also collected. The majority of the nurses had a positive total score of patient …

Valsalva Maneuver To Decrease Pain Intensity During Arteriovenous Fistula Insertion In Hemodialysis Patients, Mahruri Saputra, Ikhsanuddin Ahmad Harahap, Sutomo Kasiman

Jurnal Keperawatan Indonesia

AV fistula allows external vascular access for hemodialysis patients. Because hemodialysis patients experience puncture wounds and stabbing pain approximately 300 times a year, development of methods to decrease pain intensity are of great importance. Some techniques, such as the Valsalva maneuver, are known to reduce pain. This study aims to assess the effect of the Valsalva maneuver on decreasing the intensity of AV fistula pain in patients receiving hemodialysis. The quasi-experimental research of pre and post without control applying consecutive sampling to get as many as 63 respondents. Pain intensity was measured by using the Numerical Pain Rating Scale (NPRS). …

Living Room Ventilation And Urban Environmental Health Case In Dki Jakarta, Evi Frimawaty, Muhammad Mundzir Kamiluddin

Journal of Environmental Science and Sustainable Development

In developing countries, on average, one out of five children die from pneumonia. Death from pneumonia is most prevalent in Southeast Asia, particularly in Indonesia. Many factors can cause pneumonia. In Jakarta, the prevalence period of pneumonia in toddlers reached 19.6‰, which is higher than the national prevalence period of only 18.5‰. This study used a cross-sectional research design to analyze the relationship between disease prevalence and risk factors simultaneously. Results showed that, out of 721 children under 5 years old investigated in this study, 31 toddlers suffered from pneumonia. Furthermore, bivariate analysis showed that two variables were related to …