Medicine and Health Sciences Commons^™

Adherence Of Nurse Practitioners To Jnc 8 Guidelines, Stephanie E. Averette

Theses and Dissertations

The purposes of the evidence based project were to implement a best practice algorithm for treatment of hypertension (HTN) based on JNC 8 guidelines; compare nurse practitioners HTN pharmacotherapy prescribing practices before and after implementation of a HTN algorithm; and compare hypertensive adult patients’ systolic blood pressure (SBP) readings before and after the implementation of a HTN algorithm. A quasi-experimental pre-test and post-test design was used to collect and analyze data from 61 patient charts to compare nurse practitioner adherence to JNC 8 guidelines for NPs’ prescribing practices and subjects’ blood pressure (BP) outcome before and after the implementation of …

Genetic Diversity And Population Structure Of The African Catfish, Clarias Gariepinus (Burchell, 1822) In Kenya: Implication For Conservation And Aquaculture, James E. Barasa, Sinebongo Mdyogolo, Romulus Abila, Johannes Paul Grobler, Robert A. Skilton, Hesmari Bindeman, Moses Ndotono Njahira, Emily J. Chemoiwa, Otto G. Dangasuk, Boaz Kaunda-Arara, Erik Verheyen

All Peer-Reviewed Publications

African catfish, Clarias gariepinus, is an important species in aquaculture and fisheries in Kenya. Mitochondrial D-loop control region was used to determine genetic variation and population structure in samples of C. gariepinus from 10 sites including five natural populations (Lakes Victoria (LVG), Kanyaboli (LKG), Turkana (LTA), Baringo (LBA) and Jipe (LJP), and five farms (Sangoro Aquaculture Center (SAN), Sagana Aquaculture Centre (SAG), University of Eldoret Fish Farm (UoE), Kibos Fish Farm (KIB), and Wakhungu Fish Farm (WKU)) in Kenya. Similarly, samples from eight localities (four natural populations: LVG/LKG, LTA, LBA, and four farmed: SAN, SAG, KIB, UoE) were genotyped using …

Impact Of Cross-Level Measurement Noninvariance On Hospital Rankings Based On Patient Experiences With Care In 7 European Countries, Benedict O. Orindi, Emmanuel Lesaffre, Walter Sermeus, Luk Bruyneel

All Peer-Reviewed Publications

Background: Hospital-level findings on patient experiences with care are increasingly reported publicly. A critical aspect left unexamined is the commonality of composite measures of patient experiences across different groups of patients, nursing units, hospitals, and countries. Absence of commonality is termed measurement noninvariance and is hypothesized to have a strong impact on performance assessment. Aim: The aim of this study is to examine measurement invariance across groups and levels under study (patients, nursing units, hospitals, and countries) and illustrate the degree to which this method of analysis impacts hospital rankings. Research Design: Data were collected from 11,289 patients in 7 …

Predicting Rift Valley Fever Inter-Epidemic Activities And Outbreak Patterns: Insights From A Stochastic Host-Vector Model, Sansao A. Pedro, Shirley Abelman, Henri E.Z. Tonnang

All Peer-Reviewed Publications

Rift Valley fever (RVF) outbreaks are recurrent, occurring at irregular intervals of up to 15 years at least in East Africa. Between outbreaks disease inter-epidemic activities exist and occur at low levels and are maintained by female Aedes mcintoshi mosquitoes which transmit the virus to their eggs leading to disease persistence during unfavourable seasons. Here we formulate and analyse a full stochastic host-vector model with two routes of transmission: vertical and horizontal. By applying branching process theory we establish novel relationships between the basic reproduction number, R0, vertical transmission and the invasion and extinction probabilities. Optimum climatic conditions and presence …

Complete Genome Sequences Of Nine Enterovirus D68 Strains From Patients Of The Lower Hudson Valley, New York, 2016, Weihua Huang, Changhong Yin, Jian Zhuge, Taliya Farooq, Esther C. Yoon, Sheila M. Nolan, Donald Chen, John T. Fallon, Guiqing Wang

NYMC Faculty Publications

Complete genome sequences of nine enterovirus D68 (EV-D68) strains from patients in New York were obtained in 2016 by metagenomic next-generation sequencing. Comparative genomic analysis suggests that a new subclade B3, with ~4.5% nucleotide divergence from subclade B1 strains causing the 2014 outbreak, is circulating in the United States in 2016.

Reducing Annual Emergency Department Visits Through Improved Asthma Discharge Teaching, Alvin R. Walters

Master's Projects and Capstones

Asthma is the most common pediatric condition in the United States, and a child's home environment plays an impactful role in the triggering of asthma exacerbations (Harper et al., 2015). Current emergency department (ED) ideology focuses on symptoms management, with little time spent on education, and virtually no attention paid to the home environment. Evidence shows that programs which incorporate home environmental assessments into asthma patient education produce a reduction in annual ED visits, fewer missed school days, decreased use of rescue medications, and improvements in quality of life (Gruber K.J., et al, 2016, Hollenbach J.P. & Cloutier M.M., 2015, …

In Reply To Balon And Morreale, To Flaherty, And To Olds, Edward C. Halperin, Robert B. Goldberg

NYMC Faculty Publications

No abstract provided.

Effect Of Time Duration Of Digestion/Decontamination Technique On Yield Of Mycobacteria And Contamination Rates From Sterile Body Fluids (Pleural And Ascitic Fluid) And Pus Specimens, Samreen Shafiq, Faryal Saleem, Kauser Jabeen, Joveria Farooqi, Warda Alam, Sadia Hanif, Shazia Ali, Sadia Shakoor, Rumina Hasan

Department of Pathology and Laboratory Medicine

Background:
Duration of digestion/decontamination has a considerable impact on yield of mycobacteria especially from sterile body fluids and pus specimens. Additionally, duration of digestion/decontamination affects the contamination rates. This study evaluates the effect of digestion/decontamination protocol for 15 and 20min versus inoculation of media directly from the sample on contamination rates as well as the yield of mycobacteria from pus and sterile fluids other than cerebrospinal fluids.
Methods:
Pleural fluid (n=60), pus (n=48) and ascitic fluid (n=12) specimens were cultured for mycobacteria and evaluated for contamination and mycobacterial yield using three different processing methodologies: without digestion/decontamination with 5% NaOH-NALC (D/D), …

Muscle Specific Nucleus Ambiguus Neurons Isolation And Culturing, Ignacio Hernandez-Morato, Michael J. Pitman, Sansar Sharma

NYMC Faculty Publications

BACKGROUND: Peripheral nerve injury leads to a regenerative state. However, the reinnervation process is highly non-selective. Growing axons are often misrouted and establish aberrant synapsis to abductor or adductor muscles. Determining the complex properties of abductor and adductor motoneurons in a neuron culture, may lay the groundwork for future studies on axon guidance, leading to a clinical treatment for a selective reinnervation. NEW METHOD: In the present study we develop a neuron culture protocol to isolate recurrent laryngeal nerve abductor and adductor motoneurons in order to study their unique properties. Comparison with existing methods the best period to perform the …

Resuscitation Of Potentially Stillborn Periviable Neonates: Who Lives, Who Dies And Who Gets Missed?, Edmund F. La Gamma, J Lena Kim, Shetal Shah

NYMC Faculty Publications

No abstract provided.

Selective Inhibition Of Metabotropic Glutamate Type 1 Alpha Receptor (Mglur1Α) Reduces Cell Proliferation And Migration Following Status Epilepticus In Early Development, L K. Friedman, A Sharma, M Corcia, T Webster, L Qazi, D Simsovits, Y Khalil, S Hu, M Kantrowitz, H Hong

NYMC Faculty Publications

The present study examined whether a single or multiple episode(s) of status epilepticus induced with kainic acid (KA) during the first 3 weeks of postnatal (P) development would aberrantly stimulate proliferation zones that alters migration to potentially injured areas and whether they would be blocked by selective Group I mGluR antagonists. mGluR1α (LY367385) and mGluR5 (MPEP) antagonists were administered 2h following KA-induced status epilepticus and animals were examined after 7days. Proliferating cells of the subventricular zone (SVZ), third ventricle, hippocampus, amygdala cortical complex were analyzed with the proliferative marker, Ki67; and two complementary retrograde dye tracers. Proliferation increased in extrahippocampal …

Delivery Room Hospice, Lyndsey R. Garbi, Shetal Shah, Edmund F. La Gamma

NYMC Faculty Publications

UNLABELLED: Advances in perinatal science over the past five decades have reduced the practical 'threshold of viability' by approximately one week every 10 years such that survivors are expected as early as 22 weeks. Ethical standards regarding treatment of this periviable patient population remain enigmatic. CONCLUSION: We review limitations in the current ethical rationale for caring for these infants in the delivery room and introduce an alternative utilising a delivery room hospice care approach involving the administration of opioids.

The Ep3 Receptor Regulates Water Excretion In Response To High Salt Intake, Shoujin Hao, Annmarie Dellipizzi, Mariana Quiroz-Munoz, Houli Jiang, Nicholas R. Ferreri

NYMC Faculty Publications

The mechanisms by which prostanoids contribute to the maintenance of whole body water homeostasis are complex and not fully understood. The present study demonstrates that an EP3-dependent feedback mechanism contributes to the regulation of water homeostasis under high-salt conditions. Rats on a normal diet and tap water were placed in metabolic cages and given either sulprostone (20 μg·kg·day) or vehicle for 3 days to activate EP3 receptors in the thick ascending limb (TAL). Treatment was continued for another 3 days in rats given either 1% NaCl in the drinking water or tap water. Sulprostone decreased expression of cyclooxygenase 2 (COX-2) …

Changes In Neurotrophic Factors Of Adult Rat Laryngeal Muscles During Nerve Regeneration, Ignacio Hernandez-Morato, Sansar Sharma, Michael J. Pitman

NYMC Faculty Publications

Injury to the recurrent laryngeal nerve (RLN) leads to the loss of ipsilateral laryngeal fold movement, with dysphonia, and occasionally dysphagia. Functional movement of the vocal folds is never restored due to misrouting of regenerating axons to agonist and antagonist laryngeal muscles. Changes of neurotrophic factor expression within denervated muscles occur after nerve injury and may influence nerve regeneration, axon guidance and muscle reinnervation. This study investigates the expression of certain neurotrophic factors in the laryngeal muscles during the course of axonal regeneration using RT-PCR. The timing of neurotrophic factor expression was correlated to the reinnervation of the laryngeal muscles …

Phld 9130 - Public Health Research Methods, Yelena N. Tarasenko

College of Public Health: Faculty Syllabi (2014-2021)

The course focuses on the concepts and methods of health services and public health research. It is structured around the primary stages of research proposal development. It is designed for DrPH students in the College of Public Health and other graduate or professional students who have successfully completed the appropriate prerequisites. By the completion of this course, Students will acquire the necessary knowledge and skills to write a research proposal, plan for the dissertation, and develop and carry-out research projects.

Hypocretin/Orexin Peptides Alter Spike Encoding By Serotonergic Dorsal Raphe Neurons Through Two Distinct Mechanisms That Increase The Late Afterhyperpolarization, Masaru Ishibashi, Iryna Gumenchuk, Kenichi Miyazaki, Takafumi Inoue, William N. Ross, Christopher S. Leonard

NYMC Faculty Publications

UNLABELLED: Orexins (hypocretins) are neuropeptides that regulate multiple homeostatic processes, including reward and arousal, in part by exciting serotonergic dorsal raphe neurons, the major source of forebrain serotonin. Here, using mouse brain slices, we found that, instead of simply depolarizing these neurons, orexin-A altered the spike encoding process by increasing the postspike afterhyperpolarization (AHP) via two distinct mechanisms. This orexin-enhanced AHP (oeAHP) was mediated by both OX1 and OX2 receptors, required Ca(2+) influx, reversed near EK, and decayed with two components, the faster of which resulted from enhanced SK channel activation, whereas the slower component decayed like a slow AHP …

Microrna-140 Is Elevated And Mitofusin-1 Is Downregulated In The Right Ventricle Of The Sugen5416/Hypoxia/Normoxia Model Of Pulmonary Arterial Hypertension, Sachindra Raj Joshi, Vidhi Dhagia, Salina Gairhe, John G. Edwards, Ivan F. Mcmurtry, Sachin A. Gupte

NYMC Faculty Publications

Heart failure, a major cause of morbidity and mortality in patients with pulmonary arterial hypertension (PAH), is an outcome of complex biochemical processes. In this study, we determined changes in microRNAs (miRs) in the right and left ventricles of normal and PAH rats. Using an unbiased quantitative miR microarray analysis, we found 1) miR-21-5p, miR-31-5 and 3p, miR-140-5 and 3p, miR-208b-3p, miR-221-3p, miR-222-3p, miR-702-3p, and miR-1298 were upregulated (>2-fold; P < 0.05) in the right ventricle (RV) of PAH compared with normal rats; 2) miR-31-5 and 3p, and miR-208b-3p were upregulated (>2-fold; P < 0.05) in the left ventricle plus septum (LV+S) of PAH compared with normal rats; 3) miR-187-5p, miR-208a-3p, and miR-877 were downregulated (>2-fold; P < 0.05) in the RV of PAH compared with normal rats; and 4) no miRs were up- or downregulated with >2-fold in LV+S compared with RV of PAH and normal. Upregulation of miR-140 and miR-31 in the hypertrophic RV was further confirmed by quantitative PCR. …

Colorectal Cancer On The Decline, Albert B. Lowenfels, Patrick Maisonneuve, Ravi Mamtani

NYMC Faculty Publications

No abstract provided.

Deep Phylogenomics Of A Tandem-Repeat Galectin Regulating Appendicular Skeletal Pattern Formation, Ramray Bhat, Mahul Chakraborty, Tilmann Glimm, Thomas A. Stewart, Stuart A. Newman

NYMC Faculty Publications

BACKGROUND: A multiscale network of two galectins Galectin-1 (Gal-1) and Galectin-8 (Gal-8) patterns the avian limb skeleton. Among vertebrates with paired appendages, chondrichthyan fins typically have one or more cartilage plates and many repeating parallel endoskeletal elements, actinopterygian fins have more varied patterns of nodules, bars and plates, while tetrapod limbs exhibit tandem arrays of few, proximodistally increasing numbers of elements. We applied a comparative genomic and protein evolution approach to understand the origin of the galectin patterning network. Having previously observed a phylogenetic constraint on Gal-1 structure across vertebrates, we asked whether evolutionary changes of Gal-8 could have critically …

20-Hete Contributes To Ischemia-Induced Angiogenesis, Li Chen, Gregory Joseph, Frank F. Zhang, Huyen Nguyen, Houli Jiang, Katherine H. Gotlinger, John R. Falck, Jing Yang, Michal L. Schwartzman, Austin M. Guo

NYMC Faculty Publications

Angiogenesis is an important adaptation for recovery from peripheral ischemia. Here, we determined whether 20-hydroxyeicosatetraenoic acid (20-HETE) contributes to ischemia-induced angiogenesis and assessed its underlying molecular and cellular mechanisms using a mouse hindlimb-ischemia angiogenesis model. Hindlimb blood flow was measured by Laser Doppler Perfusion Imaging and microvessel density was determined by CD31 and tomato lectin staining. We found that systemic and local administration of a 20-HETE synthesis inhibitor, DDMS, or a 20-HETE antagonist, 6,15-20-HEDGE significantly reduced blood flow recovery and microvessel formation in response to ischemia. 20-HETE production, measured by LC/MS/MS, was markedly increased in ischemic muscles (91±11 vs. 8±2pg/mg …

Design Of Trials For Interrupting The Transmission Of Endemic Pathogens, Mariabeth Silkey, Tobias Homan, Nicolas Maire, Alexandra Hiscox, Richard Mukabana, Willem Takken, Thomas A. Smith

All Peer-Reviewed Publications

Background: Many interventions against infectious diseases have geographically diffuse effects. This leads to contamination between arms in cluster-randomized trials (CRTs). Pathogen elimination is the goal of many intervention programs against infectious agents, but contamination means that standard CRT designs and analyses do not provide inferences about the potential of interventions to interrupt pathogen transmission at maximum scale-up. Methods: A generic model of disease transmission was used to simulate infections in stepped wedge cluster-randomized trials (SWCRTs) of a transmission-reducing intervention, where the intervention has spatially diffuse effects. Simulations of such trials were then used to examine the potential of such designs …

Mitigation Of Nitrogen Mustard Mediated Skin Injury By A Novel Indomethacin Bifunctional Prodrug, Gabriella M. Composto, Jeffrey D. Laskin, Debra L. Laskin, Donald R. Gerecke, Robert P. Casillas, Ned D. Heindel, Laurie B. Joseph, Diane E. Heck

NYMC Faculty Publications

Nitrogen mustard (NM) is a bifunctional alkylating agent that is highly reactive in the skin causing extensive tissue damage and blistering. In the present studies, a modified cutaneous murine patch model was developed to characterize NM-induced injury and to evaluate the efficacy of an indomethacin pro-drug in mitigating toxicity. NM (20μmol) or vehicle control was applied onto 6mm glass microfiber filters affixed to the shaved dorsal skin of CD-1 mice for 6min. This resulted in absorption of approximately 4μmol of NM. NM caused localized skin damage within 1 d, progressing to an eschar within 2-3 d, followed by wound healing …

Linking Inflammation And Parkinson Disease: Hypochlorous Acid Generates Parkinsonian Poisons, Thomas M. Jeitner, Mike Kalogiannis, Boris F. Krasnikov, Irving Gomolin, Morgan R. Peltier, Graham R. Moran

NYMC Faculty Publications

Inflammation is a common feature of Parkinson Disease and other neurodegenerative disorders. Hypochlorous acid (HOCl) is a reactive oxygen species formed by neutrophils and other myeloperoxidase-containing cells during inflammation. HOCl chlorinates the amine and catechol moieties of dopamine to produce chlorinated derivatives collectively termed chlorodopamine. Here, we report that chlorodopamine is toxic to dopaminergic neurons both in vivo and in vitro Intrastriatal administration of 90 nmol chlorodopamine to mice resulted in loss of dopaminergic neurons from the substantia nigra and decreased ambulation-results that were comparable to those produced by the same dose of the parkinsonian poison, 1-methyl-4-phenylpyridinium (MPP+). Chlorodopamine was …

Female-Favorable Attenuation Of Coronary Myogenic Constriction Via Reciprocal Activations Of Epoxyeicosatrienoic Acids And Nitric Oxide, Ghezal Froogh, Jun Qin, Sharath Kandhi, Yicong Le, Houli Jiang, Meng Luo, Dong Sun, An Huang

NYMC Faculty Publications

Epoxyeicosatrienoic acids (EETs) are metabolites of arachidonic acid via CYP/epoxygenases, which are catabolized by soluble epoxide hydrolase (sEH) and known to possess cardioprotective properties. To date, the role of sEH in the modulation of pressure-induced myogenic response/constriction in coronary arteries, an important regulatory mechanism in the coronary circulation, and the issue as to whether the disruption of the sEH gene affects the myogenic response sex differentially have never been addressed. To this end, experiments were conducted on male (M) and female (F) wild-type (WT) and sEH-knockout (KO) mice. Pressure-diameter relationships were assessed in isolated and cannulated coronary arteries. All vessels …

Endostatin And Transglutaminase 2 Are Involved In Fibrosis Of The Aging Kidney, Chi Hua Lin, Jun Chen, Zhongtao Zhang, Gail V. Johnson, Arthur J. Cooper, Julianne Feola, Alexander Bank, Jonathan Shein, Heli J. Ruotsalainen, Taina A. Pihlajaniemi, Michael S. Goligorsky

NYMC Faculty Publications

Endostatin (EST), an antiangiogenic factor, is enriched in aging kidneys. EST is also an interactive partner of transglutaminase 2 (TG2), an enzyme that cross-links extracellular matrix proteins. Here we tested whether EST and TG2 play a role in the fibrosis of aging. In wild-type mice, aging kidneys exhibited a 2- to 4-fold increase in TG2 paralleled by increased cross-linked extracellular matrix proteins and fibrosis. Mice transgenic to express EST showed renal fibrosis at a young age. One-month delivery of EST via minipumps to young mice showed increased renal fibrosis that became more robust when superimposed on folic acid-induced nephropathy. Upregulated …

Association Of Plasma Cytokines With Radiological Recovery In Pulmonary Tuberculosis Patients, Najeeha Talat Iqbal, Rabia Hussain, Firdaus Shahid, Ghaffar Dawood

Department of Paediatrics and Child Health

Background: The characterization of tuberculosis (TB) patients as slow or fast responders post anti-TB treatment has always been a matter of tremendous interest as slow responders are most likely to relapse and/or develop complications. Pulmonary tissue healing as assessed with radiology is the only available tool for tissue recovery but is not predictive at intake. The objective of the current study was to assess biomarkers associated with fast and slow recovery in TB patients at recruitment.Methods: Pulmonary TB patients (N=15) were assessed for radiological recovery serially in parallel with clinical signs and symptoms, hematological parameters, and plasma cytokines at 0months, …

Molecular Identification Of Different Trypanosome Species And Subspecies In Tsetse Flies Of Northern Nigeria, Clement Isaac, Marc Ciosi, Alana Hamilton, Kathleen Maria Scullion, Peter Dede, Igho Benjamin Igbinosa, Oyebiguwa Patrick Goddey Nmorsi, Dan Masiga, C. Michael R. Turner

All Peer-Reviewed Publications

Background: Animal African Trypanosomiasis (AAT) is caused by several species of trypanosomes including Trypanosoma congolense, T. vivax, T. godfreyi, T. simiae and T. brucei. Two of the subspecies of T. brucei also cause Human African Trypanosomiasis. Although some of them can be mechanically transmitted by biting flies; these trypanosomes are all transmitted by tsetse flies which are the cyclical vectors of Trypanosoma congolense, T. godfreyi, T. simiae and T. brucei. We present here the first report assessing the prevalence of trypanosomes in tsetse flies in Nigeria using molecular tools. Methods: 488 tsetse flies of three species, Glossina palpalis palpalis, G. …

How Diet Intervention Via Modulation Of Dna Damage Response Through Micrornas May Have An Effect On Cancer Prevention And Aging, An In Silico Study, Felicia Carotenuto, Maria C. Albertini, Dario Coletti, Alessandra Vilmercati, Luigi Campanella, Zbigniew Darzynkiewicz, Laura Teodori

NYMC Faculty Publications

The DNA damage response (DDR) is a molecular mechanism that cells have evolved to sense DNA damage (DD) to promote DNA repair, or to lead to apoptosis, or cellular senescence if the damage is too extensive. Recent evidence indicates that microRNAs (miRs) play a critical role in the regulation of DDR. Dietary bioactive compounds through miRs may affect activity of numerous genes. Among the most studied bioactive compounds modulating expression of miRs are epi-gallocatechin-3-gallate, curcumin, resveratrol and n3-polyunsaturated fatty acids. To compare the impact of these dietary compounds on DD/DDR network modulation, we performed a literature search and an in …

2016-01-A3dsrinp-Csc-Sta-Cmb-522-Bps-542, Raymond Pulver, Neal Buxton, Xiaodong Wang, John Lucci, Jean Yves Hervé, Lenore Martin

Bioinformatics Software Design Projects

Cholesterol is carried and transported through bloodstream by lipoproteins. There are two types of lipoproteins: low density lipoprotein, or LDL, and high density lipoprotein, or HDL. LDL cholesterol is considered “bad” cholesterol because it can form plaque and hard deposit leading to arteries clog and make them less flexible. Heart attack or stroke will happen if the hard deposit blocks a narrowed artery. HDL cholesterol helps to remove LDL from the artery back to the liver.

Traditionally, particle counts of LDL and HDL plays an important role to understanding and prediction of heart disease risk. But recently research suggested that …

Gasifier As A Cleaner Cooking System In Rural Kenya, M. Njenga, M. Iiyama, R. Jamnadass, H. Helander, L. Larsson, J. De Leeuw, H. Neufeldt, K. Röing De Nowina, C. Sundberg

All Peer-Reviewed Publications

Global demand for wood fuel energy is high and rising due to population increases, particularly in sub-Saharan Africa, where firewood and charcoal are the main sources of cooking energy. Inefficient cooking techniques consume large amounts of fuel and create indoor pollution, with negative health impacts particularly among women and small children. Efficient cooking stoves can potentially save fuel and reduce the health risks of smoke in the kitchen. This study compared the ease of use, energy consumption, fuel use efficiency and gas and particle emissions of a small-scale gasifier cooking stove with that of a traditional three-stone stove and an …