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Full-Text Articles in Medicine and Health Sciences

The Effect Of Nitrate On The Release Of Glucose Into The Hemolymph Of Crayfish, Procambarus Clarkii, Alec R. Flores, Lee Kats, David Green, Gary Bucciarelli Apr 2016

The Effect Of Nitrate On The Release Of Glucose Into The Hemolymph Of Crayfish, Procambarus Clarkii, Alec R. Flores, Lee Kats, David Green, Gary Bucciarelli

Seaver College Research And Scholarly Achievement Symposium

Pollutants can change the behaviors of invertebrate stream animals. The level of one common pollutant, nitrate (NO3), has been rising in many local rivers and streams throughout the United States due to increases in anthropogenic sources. Nitrate, heavily regulated for its toxic effects to human health, namely the aiding in the development of cancer, also dangerously affects aquatic species by converting oxygen-carrying pigments to forms that are incapable of carrying oxygen. To understand the stress effects of ambient nitrate exposure on P. clarkii, we collected hemolymph samples from red swamp crayfish (Procambarus clarkii) over a 48-hour period after exposure to …


The Effect Of Tommy John (Ucl) Reconstructive Surgery On A Pitcher’S Arm And Career Progression, Jack Grant Apr 2016

The Effect Of Tommy John (Ucl) Reconstructive Surgery On A Pitcher’S Arm And Career Progression, Jack Grant

Honors Projects in Mathematics

Injuries have plagued professional athletes since their sports have been in existence. The examination of how teams can diminish the side effects of the injuries en route to a speedy recovery remains an evolving process and a topic of concern for all. Injury preventative tactics have been implemented by coaching staffs and various training personnel. Major League Baseball (MLB) pitchers are noticing an increase in the number of surgeries performed each year. The tearing of the ulnar collateral ligament (UCL) in the elbow has become a predominant injury among pitchers in the MLB. Reconstructive surgery, also known as Tommy John …


The Dawn Of De-Extinction. Are You Ready? Ted Talk Annotated Resource List, Benjamin Paul Watson Apr 2016

The Dawn Of De-Extinction. Are You Ready? Ted Talk Annotated Resource List, Benjamin Paul Watson

Undergraduate Research Award

No abstract provided.


Ua42 Regional Review: Reaching New Heights In The Region Spring 2016, Heather Garcia , Editor Apr 2016

Ua42 Regional Review: Reaching New Heights In The Region Spring 2016, Heather Garcia , Editor

Regional Campuses Publications

No abstract provided.


2016 Research Week, Linda Gardiner, David Owerbach Apr 2016

2016 Research Week, Linda Gardiner, David Owerbach

Office of Research Institutional Research and Scholarship

RESEARCH WEEK 2016 TABLE OF CONTENTS

ADMINISTRATION RESEARCH COMMITTEES ACTIVITY SCHEDULE

LETTERS OF ENDORSEMENT

GENERAL SESSION

FACULTY, STAFF, AND STUDENT POSTER PRESENTATIONS

FACULTY AND STUDENT ORAL PRESENTATIONS


Santa Clara Magazine, Volume 57 Number 2, Spring 2016, Santa Clara University Apr 2016

Santa Clara Magazine, Volume 57 Number 2, Spring 2016, Santa Clara University

Santa Clara Magazine

20 - LET THERE BE LIGHT Frank Cepollina ’59—the NASA maverick who saved Hubble. By Robert Zimmerman.

28 - LIKE NO PLACE ON EARTH Talking with John A. Sobrato ’60 about building Silicon Valley—literally. By Michael S. Malone ’75, MBA ’77.

32 - DISRUPTION IN THE HOUSE Allison Kopf ’11 just won one of the premier startup competitions on the planet. She’s making the Google Analytics of greenhouses. By Ed Cohen.

34 - AN AMERICAN STORY A few words from the remarkable life of Francisco Jiménez ’66. By Steven Boyd Saum.

38 - DR. JEROME HE was a man of …


Better Together Apr 2016

Better Together

DePaul Magazine

Faculty have taken full advantage of the university's innovative intercollegiate grant program, and the resulting research is as interesting and diverse as the collaborators themselves. What is resulting is research on "Patient and Primary Care Provider Perspectives on Recreational and Therapeutic Cannabis Use Within a Changing Socioculltural and Political Context;" a new minor in climate change science and policy; a new class, Communication, Coding and Entrepreneurship; brain inflammation research; and the project "Cosmology Meets Continental Philosophy: Natural Laws and Why There Is Something Rather Than Nothing"


Analyzing Shared Value And Social Business Principles: A Case Study Of Honeydrop Beverages And Seven Hills Foundation, Sarah Dys, Maya Grevatt, Brianna Mirabile Apr 2016

Analyzing Shared Value And Social Business Principles: A Case Study Of Honeydrop Beverages And Seven Hills Foundation, Sarah Dys, Maya Grevatt, Brianna Mirabile

School of Professional Studies

Can the business world come together with the nonprofit world to create systems to lift low income women, children, and families out of poverty? The following report aims to show how the changing principles of business have the potential to serve international populations living on less than $2USD per day. For-profit organizations working with citizen sector organizations instead of giving charity provides a sustainable model to connect profit maximization with social good.

Honeydrop Beverages is a company based out of New York that produces lemonade sweetened with honey. Their products do not contain any refined products, only using fresh and …


Nursing [Career Paper], Hannah Garrett Apr 2016

Nursing [Career Paper], Hannah Garrett

Undergraduate Research Award

No abstract provided.


Doc Wayne Youth Services, Inc. Capstone Project Youth Employment And Mentoring, Bongani T. Jeranyama, Zhengjun Liu, Sarah Parsons Apr 2016

Doc Wayne Youth Services, Inc. Capstone Project Youth Employment And Mentoring, Bongani T. Jeranyama, Zhengjun Liu, Sarah Parsons

School of Professional Studies

In Boston, Massachusetts, young adults age 16-19 who have dropped out of high school have a very high unemployment rate of 43.8%. Additionally, in the United States of America the difference between a young adult with a high school diploma as opposed to a young adult without a high school diploma in terms of weekly income is $180 USD; between a young adult with a high school diploma versus a young professional with a bachelor’s degree is $433 USD. These numbers demonstrate the need for services that improve academic achievement, job readiness and preparedness, and youth mentorship for struggling young …


A Nonprofit Model In A For-Profit World: A Closer Look At Sheltered Workshops And Sustainability As An Employee Run Business, Bing Jang, Mitchell Perry, Nikolin Vangjeli, Laura Ducharme Apr 2016

A Nonprofit Model In A For-Profit World: A Closer Look At Sheltered Workshops And Sustainability As An Employee Run Business, Bing Jang, Mitchell Perry, Nikolin Vangjeli, Laura Ducharme

School of Professional Studies

Historically, society has tended to isolate and segregate individuals with intellectual and developmental disabilities. Despite improvements such forms of discrimination continue to be a serious social problem. On October 11, 2011, the Department of Justice began an investigation into several state’s systems of providing vocational services to individuals with intellectual and developmental disabilities. This action came about due to several states being out of compliance around Title II of the Americans with Disabilities Act (ADA). Title II of the ADA prohibits discrimination on the basis of disability for all services, programs and activities provided to the public by state and …


Mechanistic Binding Insights For 1-Deoxy-D-Xylulose-5-Phosphatesynthase, The Enzyme Catalyzing The First Reaction Of Isoprenoid Biosynthesis In The Malaria-Causing Protists, Plasmodium Falciparum And Plasmodium Vivax, Matthew R. Battistini, Christopher Shoji, Sumit Handa, Leonid Breydo, David J. Merkler Apr 2016

Mechanistic Binding Insights For 1-Deoxy-D-Xylulose-5-Phosphatesynthase, The Enzyme Catalyzing The First Reaction Of Isoprenoid Biosynthesis In The Malaria-Causing Protists, Plasmodium Falciparum And Plasmodium Vivax, Matthew R. Battistini, Christopher Shoji, Sumit Handa, Leonid Breydo, David J. Merkler

Chemistry Faculty Publications

We have successfully truncated and recombinantly-expressed 1-deoxy-D-xylulose-5-phosphate synthase (DXS) from both Plasmodium vivax and Plasmodium falciparum. We elucidated the order of substrate binding for both of these ThDP-dependent enzymes using steady-state kinetic analyses, dead-end inhibition, and intrinsic tryptophan fluorescence titrations. Both enzymes adhere to a random sequential mechanism with respect to binding of both substrates: pyruvate and D-glyceraldehyde-3-phosphate. These findings are in contrast to other ThDP-dependent enzymes, which exhibit classical ordered and/or ping-pong kinetic mechanisms. A better understanding of the kinetic mechanism for these two Plasmodial enzymes could aid in the development of novel DXS-specific inhibitors that might prove useful …


Development And Use Of A Tablet-Based Resuscitation Sheet For Improving Outcomes During Intensive Patient Care, Wasif Bokahri, Vimla L. Patel, Ashish Amresh, Ayan Sen Apr 2016

Development And Use Of A Tablet-Based Resuscitation Sheet For Improving Outcomes During Intensive Patient Care, Wasif Bokahri, Vimla L. Patel, Ashish Amresh, Ayan Sen

Publications

Data documentation from resuscitation events in hospitals, termed "code blue" events, utilizes a paper form, which is institution-specific. Problems with data capture and transcription exists, due to the challenges of dynamic documentation of patient, event and outcome variables as the code blue event unfolds. We hypothesize that an electronic version of code blue real-time data capture would lead to improved resuscitation data transcription, and enable clinicians to address deficiencies in quality of care. To this effect, we present the design of a tablet-based application and its use by 20 nurses at the Mayo Clinic hospital. The results showed that the …


Research: South Dakota State University, Spring 2016, Christie Delfanian, Emily Weber Apr 2016

Research: South Dakota State University, Spring 2016, Christie Delfanian, Emily Weber

State of Discovery South Dakota State University's Research Magazine

CONTENTS:

Genome Researcher investigate impact of L1 jumping genes [Page] 2
CRP funds important resource for hunters [Page] 3
Nurse-researchers help health-care facilities develop, improve tobacco-free policies [Page] 3
MS: Exercise scientist improves movement, quality of life for MS patients [Page] 5
Plant scientists defend South Dakota crops against diseases [Plant] 7
Investigating influenza D virus earns doctoral student scholarship [Page] 9
Steel shavings trap phosphorous, protecting water quality [Page] 10


Real-Time Netnography: Rejecting The Passive Shift, Leesa Costello, Marie-Louise Mcdermott Apr 2016

Real-Time Netnography: Rejecting The Passive Shift, Leesa Costello, Marie-Louise Mcdermott

Research outputs 2014 to 2021

Although netnography emerged in the 1990s, it is a term unfamiliar to many ethnographers and is still touted as a new methodology. Once explained, ethnographers often understand it in terms of online ethnography. While this is helpful, netnography, however, offers a set of steps and analytic approaches that can be applied across a spectrum of involvement online. Its focus is on gaining entree to an online community, distinguishing between participant observation and nonparticipant observation.


Uncovering Local Trends In Genetic Effects Of Multiple Phenotypes Via Functional Linear Models, Olga A. Vsevolozhskaya, Dmitri V. Zaykin, David A. Barondess, Xiaoren Tong, Sneha Jadhav, Qing Lu Apr 2016

Uncovering Local Trends In Genetic Effects Of Multiple Phenotypes Via Functional Linear Models, Olga A. Vsevolozhskaya, Dmitri V. Zaykin, David A. Barondess, Xiaoren Tong, Sneha Jadhav, Qing Lu

Biostatistics Faculty Publications

Recent technological advances equipped researchers with capabilities that go beyond traditional genotyping of loci known to be polymorphic in a general population. Genetic sequences of study participants can now be assessed directly. This capability removed technology-driven bias toward scoring predominantly common polymorphisms and let researchers reveal a wealth of rare and sample-specific variants. Although the relative contributions of rare and common polymorphisms to trait variation are being debated, researchers are faced with the need for new statistical tools for simultaneous evaluation of all variants within a region. Several research groups demonstrated flexibility and good statistical power of the functional linear …


Mcat: Development Of A Generic Mhealth Tool For Continuous Assessment, Automatic Intervention, And Analysis, A B M Kowser Patwary Apr 2016

Mcat: Development Of A Generic Mhealth Tool For Continuous Assessment, Automatic Intervention, And Analysis, A B M Kowser Patwary

Master's Theses (2009 -)

Use of mobile health (mHealth) technology for behavioral and psychological studies through continuous assessment and intervention is found to be effective. Also, the use of smartphone has increased rapidly last few years, as well as its uses for health support. mHealth research is applied for smoking cessation, motivating cancer survivors and mentoring peers for social engagement. While in most settings researchers are developing their own intervention and assessment tool for each different research. In this study mHealth research is applied and generalized across a range of applications, including smoking cessation, motivating cancer survivors and mentoring peers to improve social engagement. …


Least-Squares Mapping From Kinematic Data To Acoustic Synthesis Parameters For Rehabilitative Acoustic Learning, Xiangyu Zhou Apr 2016

Least-Squares Mapping From Kinematic Data To Acoustic Synthesis Parameters For Rehabilitative Acoustic Learning, Xiangyu Zhou

Master's Theses (2009 -)

Thousands of people suffer from dysarthria resulting from neurological injury of the motor component of the motor-speech system, and need to rely on alternative methods to communicate in daily life, such as body language or text-to-speech [1] . However, there are currently very few effective rehabilitative therapies for helping these patients improve their speech. Because of this, research is needed to develop better rehabilitative therapies. One such area of research is the use of involuntary acoustic learning. The Speech and Swallowing lab at Marquette University has an Electromagnetic Articulography (EMA) system to collect kinematic data and a software system called …


Quality And Context-Aware Smart Health Care: Evaluating The Cost-Quality Dynamics, Nirmalya Roy, Christine Julien, Archan Misra, Sajal Das Apr 2016

Quality And Context-Aware Smart Health Care: Evaluating The Cost-Quality Dynamics, Nirmalya Roy, Christine Julien, Archan Misra, Sajal Das

Research Collection School Of Computing and Information Systems

Many emerging pervasive health-care applications require the determination of a variety of context attributes of an individual's activities and medical parameters and her surrounding environment. Context is a high-level representation of an entity's state, which captures activities, relationships, capabilities, etc. In practice, high-level context measures are often difficult to sense from a single data source and must instead be inferred using multiple sensors embedded in the environment. A key challenge in deploying context-driven health-care applications involves energy-efficient determination or inference of high-level context information from low-level sensor data streams. Because this abstraction has the potential to reduce the quality of …


Multicollinearity In Regression Analyses Conducted In Epidemiologic Studies, Kristina Vatcheva, Minjae Lee, Joseph B. Mccormick, Mohammad H. Rahbar Apr 2016

Multicollinearity In Regression Analyses Conducted In Epidemiologic Studies, Kristina Vatcheva, Minjae Lee, Joseph B. Mccormick, Mohammad H. Rahbar

School of Mathematical & Statistical Sciences Faculty Publications

The adverse impact of ignoring multicollinearity on findings and data interpretation in regression analysis is very well documented in the statistical literature. The failure to identify and report multicollinearity could result in misleading interpretations of the results. A review of epidemiological literature in PubMed from January 2004 to December 2013, illustrated the need for a greater attention to identifying and minimizing the effect of multicollinearity in analysis of data from epidemiologic studies. We used simulated datasets and real life data from the Cameron County Hispanic Cohort to demonstrate the adverse effects of multicollinearity in the regression analysis and encourage researchers …


Volume 08, Meghan Enzinna, Casey Dawn Gailey, Raven Collins, Chiara Enriquez, Amelia Mcconnell, Alexander Morton, Emma Beckett, Leah G. Parr, Briana Adhikusuma, Taylor Embrey, Rowan Davis, Danielle Sisson, Bianca Cherry, Melissa Cacho, Chloe Woodward, Catherine Rollins, Carson Reeher, Landon Cooper, Haley Vasquez, Marlisha Stewart, Eric Whitehead, Sabrina Walker, James Bates Apr 2016

Volume 08, Meghan Enzinna, Casey Dawn Gailey, Raven Collins, Chiara Enriquez, Amelia Mcconnell, Alexander Morton, Emma Beckett, Leah G. Parr, Briana Adhikusuma, Taylor Embrey, Rowan Davis, Danielle Sisson, Bianca Cherry, Melissa Cacho, Chloe Woodward, Catherine Rollins, Carson Reeher, Landon Cooper, Haley Vasquez, Marlisha Stewart, Eric Whitehead, Sabrina Walker, James Bates

Incite: The Journal of Undergraduate Scholarship

Introduction from Interim Dean Dr. Jennifer Apperson

Indigenous Peoples and the Modern Era by Meghan Enzinna

"Who Says": How Selena Gomez and the Scene Attempt to Subvert the Popular Standards of Beauty by Casey Dawn Gailey

Art by Raven Collins

Meltdown on Social Media: Amy's Baking Company Meets Kitchen Nightmares by Nathena Haddrill

Art by Chiara Enriquez

Design by Amelia Mcconnell

Worth More Than a Thousand Words: A Visual Rhetorical Discussion of Virtual Reality by Examining "Clouds Over Sidra" by Alexander Morton

Design by Emma Beckett

The Sonata: An Analysis of Piano Sonata No. 14 in C Minor, K. …


One Year Later: The Politics And Stories Of Post-Earthquake Nepal, Boyer Andrew Apr 2016

One Year Later: The Politics And Stories Of Post-Earthquake Nepal, Boyer Andrew

Independent Study Project (ISP) Collection

A 7.8 magnitude earthquake struck Nepal on April 25th, 2015. This was followed by a second major earthquake of magnitude 7.3 on May 12th , 2015. These disasters took the lives of thousands of Nepali people, destroyed hundreds of thousands of structures, and displaced an estimated two million people. Immediately following the disaster, there was an outpouring of humanitarian aid from around the world. This lead to a conference where $4.1 billion USD of reconstruction funds were pledged to Nepal by international donors. Five months later, a new constitution passed into law and an informal blockade of …


Metabolomics Reveals New Mechanisms For Pathogenesis In Barth Syndrome And Introduces Novel Roles For Cardiolipin In Cellular Function, Yana Sandlers, Kelly Mercier, Wimal Pathmasiri, Jim Carlson, Susan Mcritchie, Susan Sumner, Hilary J. Vernon Mar 2016

Metabolomics Reveals New Mechanisms For Pathogenesis In Barth Syndrome And Introduces Novel Roles For Cardiolipin In Cellular Function, Yana Sandlers, Kelly Mercier, Wimal Pathmasiri, Jim Carlson, Susan Mcritchie, Susan Sumner, Hilary J. Vernon

Chemistry Faculty Publications

Barth Syndrome is the only known Mendelian disorder of cardiolipin remodeling, with characteristic clinical features of cardiomyopathy, skeletal myopathy, and neutropenia. While the primary biochemical defects of reduced mature cardiolipin and increased monolysocardiolipin are well-described, much of the downstream biochemical dysregulation has not been uncovered, and biomarkers are limited. In order to further expand upon the knowledge of the biochemical abnormalities in Barth Syndrome, we analyzed metabolite profiles in plasma from a cohort of individuals with Barth Syndrome compared to age-matched controls via 1H nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry. A clear distinction between metabolite profiles of …


Conditional Screening For Ultra-High Dimensional Covariates With Survival Outcomes, Hyokyoung Grace Hong, Jian Kang, Yi Li Mar 2016

Conditional Screening For Ultra-High Dimensional Covariates With Survival Outcomes, Hyokyoung Grace Hong, Jian Kang, Yi Li

The University of Michigan Department of Biostatistics Working Paper Series

Identifying important biomarkers that are predictive for cancer patients' prognosis is key in gaining better insights into the biological influences on the disease and has become a critical component of precision medicine. The emergence of large-scale biomedical survival studies, which typically involve excessive number of biomarkers, has brought high demand in designing efficient screening tools for selecting predictive biomarkers. The vast amount of biomarkers defies any existing variable selection methods via regularization. The recently developed variable screening methods, though powerful in many practical setting, fail to incorporate prior information on the importance of each biomarker and are less powerful in …


Investigating The Determinants Of Recycling Behavior In Youth By Using Theory Of Planned Behavior., Tejaswini Gadiraju Mar 2016

Investigating The Determinants Of Recycling Behavior In Youth By Using Theory Of Planned Behavior., Tejaswini Gadiraju

USF Tampa Graduate Theses and Dissertations

The aim of this study is to understand the determinants that influence the youth recycling behavior intention. In other words, this study wants to know the motivations and barriers behind university students’ recycling behavior. The Theory of Planned Behavior (TPB), which provides a theoretical framework for systematically identifying the determinants of recycling behavior, is the basis for this study. Along with the TPB variables, additional predictor variables (moral norm, past experience, knowledge of how and what to recycle, knowledge of consequences, and inconvenience) that can influence behavior intentions were tested in the study. Online survey was used to collect the …


Correlation Of Maximal Inspiratory Pressure To Transdiaphragmatic Twitch Pressure In Intensive Care Unit Patients, Gerald S. Supinski, Philip M. Westgate, Leigh Ann Callahan Mar 2016

Correlation Of Maximal Inspiratory Pressure To Transdiaphragmatic Twitch Pressure In Intensive Care Unit Patients, Gerald S. Supinski, Philip M. Westgate, Leigh Ann Callahan

Internal Medicine Faculty Publications

Background: Respiratory muscle weakness contributes to respiratory failure in ICU patients. Unfortunately, assessment of weakness is difficult since the most objective test, transdiaphragmatic pressure in response to phrenic nerve stimulation (PdiTw), is difficult to perform. While most clinicians utilize maximum inspiratory pressure (Pimax) to assess strength, the relationship of this index to PdiTw has not been evaluated in a large ICU population. The purpose of the present study was to assess both PdiTw and Pimax in ICU patients to determine how these indices correlate with each other, what factors influence these indices, and how well these indices predict outcomes.

Methods: …


Comparison Of Urinary Pahs Among Firefighters And Asphalt Pavers, Theodore Aquino Mar 2016

Comparison Of Urinary Pahs Among Firefighters And Asphalt Pavers, Theodore Aquino

USF Tampa Graduate Theses and Dissertations

Firefighters and asphalt pavers are exposed to polycyclic aromatic hydrocarbons (PAHs) during various work activities. The purpose of this study was to evaluate urinary PAH levels and compare these bio-monitoring levels among firefighters, asphalt pavers, and non-occupationally exposed individuals. The National Institute of Standards and Technology (NIST) urinary PAH levels were used for non-occupationally exposed controls. When compared to the NIST standard for smokers and non-smokers, firefighters demonstrated statistically significant differences in urinary concentration differences for the following metabolites: 2-OH-fluorene, 3-OH-fluorene and 1-OH-pyrene, which were lower in firefighters than the NIST mean for smokers. 1-OH-phenanthrene, 2-OH-phenanthrene and 3-OH-phenanthrene were higher …


Program And Abstracts Of The Annual Meeting Of The Georgia Academy Of Science Mar 2016

Program And Abstracts Of The Annual Meeting Of The Georgia Academy Of Science

Georgia Journal of Science

The annual meeting of the Georgia Academy of Science took place April 1-2, 2016, at Gordon State College in Barnesville, Georgia. The keynote speaker was Dr. Mike Mahan, Dean of the School of Education at Gordon State College. His presentation was entitled "Faculty Professional Development outside of Publications!" Additional presentations were provided by members of the Academy who represented the following sections: I. Biological Sciences II Chemistry III. Earth & Atmospheric Sciences IV. Physics, Mathematics, Computer Science, Engineering & Technology V. Biomedical Sciences VI. Philosophy & History of Science VII. Science Education VIII. Anthropology


Nanoparticles Composed Of Zn And Zno Inhibit Peronospora Tabacina Spore Germination In Vitro And P. Tabacina Infectivity On Tobacco Leaves, George Wagner, Victor Korenkov, Jonathan D. Judy, Paul M. Bertsch Mar 2016

Nanoparticles Composed Of Zn And Zno Inhibit Peronospora Tabacina Spore Germination In Vitro And P. Tabacina Infectivity On Tobacco Leaves, George Wagner, Victor Korenkov, Jonathan D. Judy, Paul M. Bertsch

Plant and Soil Sciences Faculty Publications

Manufactured nanoparticles (NPs) are increasingly being used for commercial purposes and certain NP types have been shown to have broad spectrum antibacterial activity. In contrast, their activities against fungi and fungi-like oomycetes are less studied. Here, we examined the potential of two types of commercially available Zn NPs (Zn NPs and ZnO NPs) to inhibit spore germination and infectivity on tobacco leaves resulting from exposure to the fungi-like oomycete pathogen Peronospora tabacina (P. tabacina). Both types of NPs, as well as ZnCl2 and bulk ZnO control treatments, inhibited spore germination compared to a blank control. ZnO ENMs …


Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner Mar 2016

Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner

Veterinary Diagnostic Laboratory Faculty Publications

Microcystins are acute hepatotoxins of increasing global concern in drinking and recreational waters and are a major health risk to humans and animals. Produced by cyanobacteria, microcystins inhibit serine/threonine protein phosphatase 1 (PP1). A cost-effective PP1 assay using p-nitrophenyl phosphate was developed to quickly assess water and rumen content samples. Significant inhibition was determined via a linear model, which compared increasing volumes of sample to the log-transformed ratio of the exposed rate over the control rate of PP1 activity. To test the usefulness of this model in diagnostic case investigations, samples from two veterinary cases were tested. In August …