Medicine and Health Sciences Commons^™

Synthesis Of Gold Nanoparticles Via Pulsed Liquid Ablation For Use In The Photodynamic Therapy Of Bacteria, Justice Ben Yosef

Masters Theses & Specialist Projects

With the ever-increasing threat of antibiotic resistant bacteria, alternative treatment methods have been developed including photodynamic therapy (PDT). Within the PDT process, photosensitizers are used to generate reactive oxygen species (ROS) and facilitate the cell termination process. This work lays the conceptual foundation for the functionalization of the photosensitizer methylene blue (MB) with gold nanoparticles (AuNPs) and INF-55 as a potential inhibitor of the AcrAB-TolC efflux pump to enhance the effectivity of the PDT process.

AuNPs were synthesized using pulsed laser ablation in an aqueous citrate solution. Both nanosecond and picosecond pulse durations, as well as both 532 nm and …

Grey Relational Analysis And Canonical Correlation Analysis Of Air Pollution In Three Kentucky Counties, Sarah Hartman

Masters Theses & Specialist Projects

Air pollution is a crucial factor that affects both the environment and public health. Various methods are available for assessing air quality and pollution levels, such as regression models, principal component analysis, and factor analysis tools. However, some of these methods present issues in multicollinearity and the nature of collected data. It is important to recognize that air pollution data is often uncertain, incomplete, and contains limited valid data points. Weather conditions and economic activities are also factors that can affect air pollution. With growing communities in Kentucky (KY), it is essential to address these factors as the state has …

Bioactivity And Structural Properties Of Hydroxyapatite On Ti6a|4v And Si(100) Surfaces By Pulsed Laser Deposition, Salizhan Kylchbekov

Masters Theses & Specialist Projects

Although biomedical implant technology is very advanced, there are still caveats in terms of biocompatibility properties because metals are inert to biological processes such as osseointegration, cell growth, and cell adhesion. This results in statistically significant complications and opens rooms for improvements. Among many techniques to improve this problem, coating the metal surface with biologically functional materials has resulted in the best performances. Hydroxyapatite (HAP), Ca_₁₀(PO_₄)_₆(OH)_₂, is the most stable form of calcium phosphates in human body environment and is functional in key processes such as ion exchange, osteoblast (bone cells) formation, …

Liquid Chromatography With Tandem Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Symone T. Whalin

Masters Theses & Specialist Projects

Bovine mastitis, caused by a wide array of pathogens, results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the bovine’s innate immune system and act as the first line of defense against mastitis. Only foundational research has been done on β-defensins’ ability to treat and prevent mastitis. There have been no analytical methods reported in the literature for analyzing β-defensins in bovine milk. This research aims to create an analytical approach to determine β-defensins in bovine milk. It is challenging to determine an analyte in a complex sample matrix, and milk is …

Inflammatory Response To Sleep Fragmentation In Skeletal, Cardiac, And Smooth Muscle Tissues In Female Mice, Patton Allen

Mahurin Honors College Capstone Experience/Thesis Projects

Sleep is a critical process that the body undergoes. When sleep is interrupted, so that an individual is awakened for some period before going back into sleep, the sleep can be described as fragmented. Studies in the past have shown that sleep fragmentation (SF) promotes an inflammatory environment, especially in the brain and peripheral tissue. However, studies have not been conducted to observe inflammatory responses in muscle. To examine this, C57BL/6J female mice were subjected to either a control group (no SF) or a SF group which involved using an automated SF chamber to disrupt sleep every 2 min over …

H-Discrete Fractional Model Of Tumor Growth And Anticancer Effects Of Mono And Combination Therapies, Kamala Dadashova

Masters Theses & Specialist Projects

In this thesis, we focus on h–discrete and h–discrete fractional representation of a pharmacokinetics-pharmacodynamics (PK-PD) model which describes tumor growth considering time on hNa, where h>0. First, we introduce some definitions, lemmas and theorems on both h–discrete and h–discrete fractional calculus in the preliminary section. In Chapter 3, we work on the PD model with delay by exam ining nabla h–discrete equations and nabla h–discrete fractional equations as well as variation of constants formulas, accordingly. We introduce our model and solve it using theorems we proved in the last section of the indicated chapter. When we do simulation for …

Ua12/2/1 College Heights Herald, Vol. 95, No. 18, Wku Student Affairs

WKU Archives Records

WKU campus newspaper reporting campus, athletic and Bowling Green, Kentucky news. This issue contains articles:

• Lamb, Cassady. Posted Up: Local Apartment Complexes Are Suing the United States Post Office
• Williams, Matthew. WKU to be Evaluated by Higher Education Consulting Firm
• Collins, Michael. Strategic Sustainability Plan to be Developed
• Stack, Madalyn. Editorial Cartoon re: Racism
• Black History Month: Past & Present
• Warner, Casey. Kentucky Canabis Laws Are Behind the Times – Marijuana
• Dobbs, Jack. Bitter Sweet – Chocolate Festival, Hospice of Southern Kentucky
• Metcalf, Taylor. Fast Fashion
• Lowe, Julianna. Sustainable On-campus Bathroom Practices, In Public or Private
• Gaylord, Kaden. WKU Softball …

Leaving Ligand Effects On Reactivity And Solubility Of Monofunctional Platinum(Ii) Anticancer Complexes, Heidi Linn Hruska Millay

Masters Theses & Specialist Projects

Monofunctional platinum(II) complexes, such as phenanthriplatin and pyriplatin, have notably different characteristics from the bifunctional anticancer complexes, such as cisplatin and oxaliplatin, which have detrimental toxicities and resistance associated with them. The unique properties of the monofunctional complexes may be exploited to target cancer cells without producing the toxic side effects associated with the current FDA-approved platinum-based anticancer drugs. To advance the understanding of these monofunctional platinum(II) complexes, this study replaced the chloride leaving ligand with an acetate group, which should increase solubility and alter the rate of reactivity with key amino acid and nucleotide targets. Phenanthriplatin and pyriplatin compounds …

Interaction Of A Platinum Triamine Complex Having A Seven-Membered Chelate Ring With N-Acetyl-Lmethionine And Guanosine 5'-Monophosphate, Jae Ko

Masters Theses & Specialist Projects

In the 1960s, Rosenberg and his colleagues confirmed the anti-cancer activity of cisplatin. Although cisplatin was capable of killing testicular cancer cells there were also serious side effects. It was necessary to find alternate ways of overcoming side effects, and soon many researchers have discovered novel platinum compounds that show similar reactivity. Recently, replacing one chloride group to a heterocyclic amine group showed significant cytotoxicity with a different binding activity than cisplatin. Previously in our lab, [Pt(Me5dien)(NO3)]+ and [Pt(Et2dien)Cl]+ have been synthesized and reacted with NAcetyl- L-methionine (N-AcMet) and Guanosine 5’-monophosphate (5’-GMP) showed unusual reactivity. Unlike most previously studied platinum …

Synthesis Of Bis(Imino)Pyridine Iron(Ii) Complexes And Development Of Bis(Imino)Pyridine Iron(Ii) Catalyzed Carbene Transfer Reactions, Ban Wang

Masters Theses & Specialist Projects

Metal catalysis of symmetric and asymmetric carbene transfer reactions has been widely applied in natural product synthesis and material science over years. Metal carbene can be easily generated from the extrusion of nitrogen under the catalysis of metal complexes to further undergo various organic reactions, O/N/C-H insertions, cycloadditions, and ylide formations. Currently, the dominant effective catalysts for carbene reactions are built with expensive precious metal, for example, rhodium, ruthenium, palladium, gold. Notably, the effective reactivity and enantioselectivity of the dirhodium(II) catalysts are researched and established over the decades. However, the use of precious metal catalysts is the major source of …

Parameter Estimation And Optimal Design Techniques To Analyze A Mathematical Model In Wound Healing, Nigar Karimli

Masters Theses & Specialist Projects

For this project, we use a modified version of a previously developed mathematical model, which describes the relationships among matrix metalloproteinases (MMPs), their tissue inhibitors (TIMPs), and extracellular matrix (ECM). Our ultimate goal is to quantify and understand differences in parameter estimates between patients in order to predict future responses and individualize treatment for each patient. By analyzing parameter confidence intervals and confidence and prediction intervals for the state variables, we develop a parameter space reduction algorithm that results in better future response predictions for each individual patient. Moreover, use of another subset selection method, namely Structured Covariance Analysis, that …

Ua12/2/2 Talisman: Movement, Wku Student Affairs

WKU Archives Records

2018 Talisman yearbook.

• Good, Hannah. Movement
• Kinser, Nicholas. Tunnel Trap
• Cozer, Claire. A Day in the Life of a Food Truck – Mike Wilson, Pop’s Street Eats
• Fletcher, Griffin. Beauty in Power – WKU Women’s Rugby Club
• Gordon, Zora. The Mixed Experience
• Hornsby, Morgan. Bonfire
• Waters, Adrianna. Mispoken – Communication Disorders
• Chu, Phi. Home Base – Jessica Barks
• Cooksey, Catrina. Rerouted – Sydney Clark, Austin Clark, Blake Perkins, Sheila Flener, Handicapped Persons
• Good, Hannah. Not Safe for Work – Prostitution
• Chu, Phi. Transfigured Night
• Carter, De’inara. Passing the Plate – International Students, Recipes
• Robb, Hayley. From Sole to Soul – …

Photochemical Investigation Of High-Valent Metal-Oxo Intermediates Containing Corrole And Light-Harvesting Porphyrin Ligands, Jonathan Malone

Masters Theses & Specialist Projects

In enzymatic and synthetic catalytic oxidations, high-valent iron-oxo intermediates play a vital role as the active oxidant. In this regard, many synthetic metal catalysts are designed as biomimetic models to resemble the active site of Cytochrome P450 enzymes (P450) which are the predominant oxidation catalysts in nature. Vitamin B12 cofactors, with a corrole-like structure corrin, are also utilized in some of the more difficult reactions in nature such as rearrangement and reductase reactions.

In this work, application of the promising photochemical method to corrolecontaining ligands systems showed much success in the generation of manganese(V)-oxo corrole intermediates using two electron-deficient corrole …

Analyzing The Efficiency Of Reforestation Efforts In Regaining Carbon Storage In A Costa Rican Cloud Forest, Elvin Irihamye

Student Research Conference Select Presentations

Forests hold an important place in offsetting carbon emissions, absorbing nearly 40 percent of man-made fossil fuel emissions every year. Over the last 100 years, the effects of deforestation have crippled our forest’s ability to store excess carbon, leading to drastic atmospheric change. Efforts to regrow deforested forests have increased rapidly to address issues like climate change. There is thus a need to analyze current efforts at regaining carbon storage, a critical component to maintaining atmospheric homeostasis. Accordingly, we traveled to the Cloudbridge Nature Reserve in San Gerardo, Costa Rica to aggregate data on the carbon storage capabilities across three …

Regional Review: Reaching New Heights In The Region Fall 2017 (Vol.4, No.1), Heather Garcia , Editor

Regional Campuses Publications

Sections include:

Save the date Chat with the Chancellor, Campus News, and Awesome Achievements for each of the three regional campuses.

How I Learned To Communicate My Inner Life With Asperger’S: Ted Talk Annotated Resource List, Meghan Rice

Undergraduate Research Award

No abstract provided.

Numerically Solving A System Of Pdes Modeling Chronic Wounds Treated With Oxygen Therapy, Stefan Stryker

Mahurin Honors College Capstone Experience/Thesis Projects

Chronic wounds such as diabetic foot ulcers are the leading cause of non-traumatic amputations in developed countries. For researchers to better understand the physiology of these wounds, a mathematical model describing oxygen levels at the wound site can be used to help predict healing responses. The model utilizes equations that are modified from work by Guffey (2015) that consists of four variables – oxygen, bacteria, neutrophils, and chemoattractant within a system of partial differential equations. Our research focuses on numerically solving these partial differential equations using a finite volume approach. This numerical solver will be important for future research in …

Cytotoxicity Of Platinum Anticancer Drugs In Mammalian Cell Lines Of Metastatic Cancer, Hosannah Evie

Mahurin Honors College Capstone Experience/Thesis Projects

With the invention of advanced technology, focus has been put on understanding and looking for potential cures for many diseases, one of which is cancer. The difference in the leaving and non-leaving ligands of the FDA approved cancer drugs contributes to the differential cell specific cytotoxic effects. These drugs such as oxaliplatin approved for colorectal cancer, cisplatin approved for testicular cancer, and their analogs were used to treat different cancer cell lines in an MTT assay. This project aims to determine how changing the molecular shape of these compounds affects their uptake and toxicity into different cell lines. The assay …

The Incredible Inventions Of Intuitive Ai: Ted Talk Annotated Resource List, Dylan Duckworth

Undergraduate Research Award

No abstract provided.

Regional Review: Reaching New Heights In The Region Spring 2017, Heather Garcia , Editor

Regional Campuses Publications

No abstract provided.

Ua12/2/2 2017 Talisman: Well Being, Wku Student Affairs

WKU Archives Records

2017 Talisman yearbook.

• Kaetzel, Kylee. Editor’s Note
• Upton, Jordan. His Hands
• Watkins, Abby. The Meal Plan – Recipes
• Eastham, Lillie. New Hieghts – Reed Mattison, Rock Climbing
• Robb, Hayley. A Life Transformed – Annabeth Welborn
• Robb, Hayley. 6:00 AM Bright & Early – Exercise
• Doyel, Rachael. Redifining Ability – Jacob Holt
• Upton, Jordan. Yom Kippur at Happy Hills Buddhist Temple
• Wegert, Sally. Inner Peace – Allison Adams, Yoga
• Mattingly, Evan. The Future’s in Your Hands – Bianca Williams, Psychics
• Frint, Hunter. Too Often Silenced – Unity Walk
• Doyel, Rachael. Changing Tides
• Wells, Spencer. Struggle & Rebirth – Trey Farlee, Musicians …

Teaching Assistant [Career Paper], Madelynn Williams

Undergraduate Research Award

No abstract provided.

47th Annual Wku Student Research Conference, Student Research Council,

Student Research Conference Select Presentations

See document below for project abstracts

Co-Localization Of Phk Γ-181 And Na-14 In Sh-Sy5y Cells, Brent Harney

Student Research Conference Select Presentations

No abstract provided.

2017 Abstracts Student Research Conference, Student Research Conference

Student Research Conference Select Presentations

No abstract provided.

How We’Ll Fight The Next Deadly Virus Ted Talk Annotated Resource List, Susie Roy

Undergraduate Research Award

No abstract provided.

Your Body Language Shapes Who You Are Ted Talk Annotated Resource List, Elizabeth Hurm

Undergraduate Research Award

No abstract provided.

Manufacturing And Engineering [Career Paper], Mahdi Alawami

Undergraduate Research Award

No abstract provided.

46th Annual Wku Student Research Conference, Student Research Council

Student Research Conference Select Presentations

See document below for project abstracts

The Dawn Of De-Extinction. Are You Ready? Ted Talk Annotated Resource List, Benjamin Paul Watson

Undergraduate Research Award

No abstract provided.