Medicine and Health Sciences Commons^™

Plant Expression Of Cocaine Hydrolase-Fc Fusion Protein For Treatment Of Cocaine Abuse, Guojun Wang, Ting Zhang, Haifeng Huang, Shurong Hou, Xiabin Chen, Fang Zheng, Chang-Guo Zhan

Molecular Modeling and Biopharmaceutical Center Faculty Publications

BACKGROUND: A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of cocaine overdose and addiction. A challenge for practical therapeutic use of this enzyme exists in the large-scale protein production and, therefore, it is interesting to identify a low-cost and feasible, sustainable source of CocH3-Fc production.

RESULTS: CocH3-Fc was transiently expressed in plant Nicotiana benthamiana leaves. The plant-expressed protein, denoted as pCocH3-Fc, was as active as that expressed in mammalian cells both in vitro and in vivo. However, compared to …

Local Intra-Uterine Ang-(1-7) Infusion Attenuates Pge2 And 6-Keto Pgf1Α In Decidualized Uterus Of Pseudopregnant Rats, K. B. Brosnihan, V. M. Pulgar, M. S. Bharadwaj, L. A. Neves, L. M. Yamaleyeva

Pharmaceutical Sciences

No abstract provided.

Developing Students As Advocates Through A Pilot Advocacy Curricular Thread Within A Pharmd Curriculum, Cortney M. Mospan, Mckenzie L. Calhoun

ETSU Faculty Works

There is a need for education about and development of advocacy skills within student pharmacists. Pharmacy literature describes experiences incorporating advocacy education within curricula; however, these are often singular, limited experiences. To increase student pharmacists’ awareness, knowledge, and skills, effective advocacy curricular threads are necessary within curricula. This paper provides rationale for the need for a greater emphasis on development of these skills within student pharmacists, evidence of curricular experiences surrounding advocacy from the literature, initial observations from a piloted curricular thread at one school, and implications for the academy.

Profile Interview With Faculty Mentor Jane Krause, Elizabeth J. Dowell, Ashley M. Schinker

Purdue Journal of Service-Learning and International Engagement

No abstract provided.

Improving Mental Health Of Elementary School Children, Alexa Proctor

Purdue Journal of Service-Learning and International Engagement

Alexa Proctor is an advanced practice student on clinical rotations with the Purdue University College of Pharmacy. In this article, she describes her experience working with Mental Health America (MHA) of Tippecanoe County and third grade children, in addition to her passion to help improve mental health in students of all ages.

Learning To Sew: A Student Pharmacist’S Service-Learning Experience, Karolina M. Grzesiak, Craig A. Vargo, Ellen M. Schellhase, Monica L. Miller, Rakhi Karwa, Sonak D. Pastakia, Beatrice Jakait

Purdue Journal of Service-Learning and International Engagement

Karolina Grzesiak is a fourth-year professional student in the College of Pharmacy at Purdue University and will earn her Doctor of Pharmacy degree in May 2017. She was raised in Poland but has called La Porte, Indiana home for the past eight years. Craig Vargo is a 2012 pharmacy graduate working as a clinical specialist pharmacist at the James Cancer Hospital at The Ohio State University Wexner Medical Center in Columbus, Ohio.

A Road Less Traveled: My Experience With Peer Advisors For Veteran Education, Mckenna Jennings

Purdue Journal of Service-Learning and International Engagement

In this article, Mckenna Jennings, a third-year PharmD student at Purdue University, describes her experience with the Peer Advisors for Veteran Education (PAVE) program, developed under the University of Michigan Military Support Programs and Networks. She also explains her motivation for involvement and the opportunity for others to become involved.

The Johns Hopkins Hospital: A Summer Internship, Adam Smith

Purdue Journal of Service-Learning and International Engagement

Adam Smith, a native of Richmond, Indiana, is an advanced pharmacy practice student in the College of Pharmacy at Purdue University. In this article, he describes how career exploration through a summer internship with The Johns Hopkins Hospital in Baltimore, Maryland solidified his desire to pursue a career in pharmacy administration.

Connecting Volunteerism To A Career: My Journey With The American Cancer Society And Pharmacy, Christine Kane

Purdue Journal of Service-Learning and International Engagement

Christine Kane is a 2016 graduate from the Purdue College of Pharmacy. In this article, she describes how two passions, the profession of pharmacy and volunteerism with the American Cancer Society, became intertwined to change the course of her career. She implores fellow students to never underestimate the impact an extracurricular activity can have on the rest of your life.

Structures Of Mithramycin Analogues Bound To Dna And Implications For Targeting Transcription Factor Fli1, Caixia Hou, Stevi Weidenbach, Kristin E. Cano, Zhonghua Wang, Prithiba Mitra, Dmitri N. Ivanov, Jürgen Rohr, Oleg V. Tsodikov

Pharmaceutical Sciences Faculty Publications

Transcription factors have been considered undruggable, but this paradigm has been recently challenged. DNA binding natural product mithramycin (MTM) is a potent antagonist of oncogenic transcription factor EWS–FLI1. Structural details of MTM recognition of DNA, including the FLI1 binding sequence GGA(A/T), are needed to understand how MTM interferes with EWS–FLI1. We report a crystal structure of an MTM analogue MTM SA–Trp bound to a DNA oligomer containing a site GGCC, and two structures of a novel analogue MTM SA–Phe in complex with DNA. MTM SA–Phe is bound to sites AGGG and GGGT on one DNA, and to AGGG and GGGA(T) …

Olanzapine For Chemotherapy-Induced Nausea And Vomiting, John B. Bossaer

ETSU Faculty Works

Excerpt: Navari and colleagues (July 14 issue)1 report on the use of olanzapine for the prevention of chemotherapy-induced nausea and vomiting.

Pharmacy Leadership And Political Advocacy Php 464, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Near Infrared Fluorescent Nanoparticles Derived From Hyaluronic Acid Improve Tumor Contrast For Image-Guided Surgery., Tanner K. Hill, Sneha S. Kelkar, Nicholas E. Wojtynek, Joshua J. Souchek, William M. Payne, Kristina Stumpf, Frank C. Marini, Aaron M. Mohs

Journal Articles: Pharmaceutical Sciences

Tumor tissue that remains undetected at the primary surgical site can cause tumor recurrence, repeat surgery, and treatment strategy alterations that impose a significant patient and healthcare burden. Intraoperative near infrared fluorescence (NIRF) imaging is one potential method to identify remaining tumor by visualization of NIR fluorophores that are preferentially localized to the tumor. This requires development of fluorophores that consistently identify tumor tissue in different patients and tumor types. In this study we examined a panel of NIRF contrast agents consisting of polymeric nanoparticle (NP) formulations derived from hyaluronic acid (HA), with either physically entrapped indocyanine green (ICG) or …

Physicochemical Properties Of Engineered Nanomaterials That Influence Their Nervous System Distribution And Effects, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

This critical review examines in vitro and in vivo evidence for the influence of engineered nanomaterial (ENM) physicochemical properties on their distribution into, and effects on, the nervous system. Nervous system applications of ENMs; exposure routes and potential for uptake; the nervous system and its barriers to ENM uptake; and the mechanisms of uptake into the nervous system and overcoming those barriers are summarized. The findings of English-language publications of studies that included at least two variations of an ENM physicochemical property and reported results of their pharmacokinetic and/or pharmacodynamic interaction with the nervous system that differed as a function …

An Arginine Finger Regulates The Sequential Action Of Asymmetrical Hexameric Atpase In The Double-Stranded Dna Translocation Motor, Zhengyi Zhao, Gian Marco De-Donatis, Chad T. Schwartz, Huaming Fang, Jingyuan Li, Peixuan Guo

Pharmaceutical Sciences Faculty Publications

Biological motors are ubiquitous in living systems. Currently, how the motor components coordinate the unidirectional motion is elusive in most cases. Here, we report that the sequential action of the ATPase ring in the DNA packaging motor of bacteriophage ϕ29 is regulated by an arginine finger that extends from one ATPase subunit to the adjacent unit to promote noncovalent dimer formation. Mutation of the arginine finger resulted in the interruption of ATPase oligomerization, ATP binding/hydrolysis, and DNA translocation. Dimer formation reappeared when arginine mutants were mixed with other ATPase subunits that can offer the arginine to promote their interaction. Ultracentrifugation …

Could The Pharmaceutical Industry Benefit From Full-Scale Adoption Of Radio-Frequency Identification (Rfid) Technology With New Regulations?, Alberto Coustasse Drph, Md, Mba, Mph, Craig Kimble Pharmd, Mba, Ms, Bcacp, Robert B. Stanton Mba, Pharmd, Mariah Naylor

Management Faculty Research

Healthcare regulators are directing attention to the pharmaceutical supply chain with the passage of the Drug Quality and Security Act (DQSA) and the Drug Supply Chain Security Act (DSCSA). Adoption of Radio-Frequency Identification (RFID) technology has the ability to improve compliance, reduce costs, and improve safety in the supply chain but its implementation has been limited; primarily because of hardware and tag costs. The purpose of this research study was to analyze the benefits to the pharmaceutical industry and healthcare system of the adoption of RFID technology as a result of newly implemented supply chain regulations. The methodology was a …

Retrospective Evaluation Of Dosing Body Weight For Unfractionated Heparin In Obese Patients, Jingyang Fan, Billee John, Emily Tesdal

SIUE Faculty Research, Scholarship, and Creative Activity

Purpose: Using adjusted body weight (AdjBW) for heparin dosing in obese patients is a common but not validated clinical practice. The purpose of this study was to evaluate whether using AdjBW in obese patients would lead to quick achievement of therapeutic activated partial thromboplastin time (aPTT) and low bleeding risk with heparin therapy.

Methods: A retrospective cohort study was conducted in patients that received heparin before and after implementation of a revised heparin protocol that utilized AdjBW for obese patients. The primary outcome was percentage of first aPTT values within the therapeutic range. Secondary outcomes included time to first therapeutic …

Medical Cannabis: Miracle Or Myth?, Blair Henry, Arnav Agarwal, Edward Chow, Hatim A. Omar, Joav Merrick

Pediatrics Faculty Publications

No abstract provided.

Lgbtq Pharmacy Guide, Jennifer Mathews

Pharmacy Faculty/Staff Publications

This guide has been developed to help pharmacists and pharmacy staff provide an inclusive and welcoming environment for their LGBTQ patients. When LGBTQ patients do not feel welcomed and included or when they experience a negative encounter at a pharmacy they are less likely to return, which may negatively impact their health if this leads to delays or non-adherence with prescribed medications. This guide will cover key concepts and LGBTQ terms as well as the health disparities faced by LGBTQ people. It includes key tips on how to best care for LGBTQ patients as well as how to make your …

Nsu Sharks Rx - Fall 2016, Nova Southeastern University

NSU Sharks Rx

No abstract provided.

Selective Anticancer Activity Of Hydroxyapatite/Chitosan-Poly(D,L)-Lactide-Co-Glycolide Particles Loaded With An Androstane-Based Cancer Inhibitor, Nenad Ignjatović, Katarina M. Penov-Gaši, Victoria M. Wu, Jovana J. Ajduković, Vesna V. Kojić, Dana Vasiljević-Radović, Maja Kuzmanović, Vuk Uskoković, Dragab Uskoković

Pharmacy Faculty Articles and Research

In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. ¹H NMR and ¹³C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the …

Advances And Perspectives In Genetics Of Congenital Thyroid Disorders, Héctor M. Targovnik, Cintia E. Citterio, Sofi Siff, Carina M. Rivolta

Pharmacy Faculty Articles and Research

"Congenital hypothyroidism (CH) is the most frequent endocrine disease in infants, affects about 1 in 3,000 newborns and is characterized by elevated levels of thyroidstimulating hormone (TSH) as a consequence of reduced thyroid function. It is also one of the most common preventable causes of cognitive and motor deficits. Prevention of CH is based on carrier identification, genetic counseling and prenatal diagnosis. In neonates a complete diagnosis of CH should include clinical examination, biochemical thyroid tests, thyroid ultrasound, radioiodine or technetium scintigraphy and perchlorate discharge test (PDT). In the last two decades, considerable progress has been made in identifying the …

September 2016, Randy Curry, Cindy Brooks

RURAL ROCKS

Rural Rocks, the Rural Health Network newsletter by the SWOSU College of Pharmacy

Use Of Parthenolide Derivatives As Antileukemic And Cytotoxic Agents, Peter A. Crooks, Craig T. Jordan, Xiaochen Wei

Pharmaceutical Sciences Faculty Patents

To read this abstract, please download this patent.

Semi-Synthetic Mithramycin Derivatives With Anti-Cancer Activity, Jürgen Rohr, Daniel Scott, Markos Leggas, Jhong-Min Chen, Oleg V. Tsodikov

Pharmaceutical Sciences Faculty Patents

Mithramycin derivatives and their pharmaceutically acceptable salts are disclosed. The mithramycin derivatives can be used in the treatment of Ewing sarcoma or other cancer or neuro-disease associated with an aberrant erythroblast transformation- specific transcription factor.

Cross-Sectional Survey Of Perceived Barriers Among Community Pharmacists Who Do Not Immunize, In Wayne County, Michigan, Sarah E. Kelling, Anthony Pattin, Abdulbaset Salim, Paul Kilgore, Steven R. Erickson

Department of Pharmacy Practice

Introduction: The goal of the study was to identify perceived barriers to implementation of vaccination services encountered by independent and small-chain community pharmacies in an urban setting.

Methods: Pharmacists in independent and small-chain pharmacies located in 29 Michigan ZIP codes were visited and asked to complete a 5- to 10-min semi-structured interview.

Results: A total of 93 independent and 12 small-chain pharmacies participated (n = 105; 61%). The pharmacies filled an average of 700 prescriptions each week with 1.1 pharmacist full-time equivalents and 57 h of technician time. The most common services that participating pharmacies provided were dispensing outpatient …

Introduction To Pharmacotherapy In Older Adults Php 444, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Secondary Data Analysis Of Informal Caregiving In A Medicare Population, Jean T. Carter

University Grant Program Reports

The purpose of the study is to describe the status of informal caregiving within a subset of the Medicare population that is receiving some of its health care in the home and patient outcomes associated with informal caregiving. Rural settings and medication issues will be a particular focus.

Postgraduate Year 1 Pharmacy Residency Accreditation Requirements And Challenges, Emmanuelle Schwartzmann, Andrea Walsh Haron, Mary Kate Mcginty, Katrin S. Fulginiti

Pharmacy Faculty Articles and Research

"The residency accreditation process can be a stress-inducing experience to many program directors. In 2014, the postgraduate year 1 (PGY1) pharmacy residency accreditation standards were updated from the previous 2005 version. The new standards were formulated to streamline program requirements with the intention of creating a more transparent accreditation process.1 The American Society of Health- System Pharmacists (ASHP) is the accrediting body for PGY1 residencies, PGY1 community pharmacy residencies, PGY1 residencies in managed care pharmacy, and PGY2 pharmacy residency programs in advanced practice areas. ASHP provides many resources to help programs to prepare for and navigate through the accreditation process …

Structural And Dynamic Analysis Of Wild And Splice Variants Human Μ-Opiod Receptors In Complex With Morphine And Ibntxa And Human Topoisomerase Ii Alpha Mutational Basis Of Amsacrine Resistance, Safaa Sader

Theses and Dissertations

Morphine prescribing is limited by its high addiction tendency and other serious effects. Recent animal’s biological studies on Mu 6TM splice variants, which mainly include G1, G2 and Mu3, supported a high safety and potency profiles of IBNtxA as potential alternative of Morphine. Nevertheless, there is no high-resolution structures of these 6TM variants, and the detailed structural features and dynamic characteristics of these splice variants remain elusive. We applied homology modeling and MD simulation to probe the structural, dynamic and ligand binding differences between the wild type (7TM) and two major truncated 6TM variants (G1 and G2). MD results underscored …