Medicine and Health Sciences Commons^™

Case Of Severe Depression With Psychotic Symptoms In Childhood, Vijaykumar Hiremath, Dr. Anusha Pogula, Dr. Kishor M

Digital Journal of Clinical Medicine

No abstract provided.

A Case Of Moderate Clinical Depression In Early Onset Parkinson’S Disease, Dr. Rakshith N R, Dr. Pranahith V. Reddy, Dr. Kishor M

Digital Journal of Clinical Medicine

No abstract provided.

A Case Of P2l2 With Scar Endometriosis With Previous Two Lscs, Dr. Punitha Nagaraj, Dr. Poornima M

Digital Journal of Clinical Medicine

No abstract provided.

A Case Of Atypical Presentation Of Radial Nerve Injury, Nikita Tripathi, Dr Adarsh Thammaiah

Digital Journal of Clinical Medicine

No abstract provided.

Urti Associated With Reuse Of Mask During Covid 19, Nikita Nallathambi, Dr. Rakesh Bs

Digital Journal of Clinical Medicine

No abstract provided.

A Case Of Ovarian Torsion, Dr.Durkha Devi K P, Dr.Poornima M

Digital Journal of Clinical Medicine

No abstract provided.

Preclinical Pharmacokinetic Studies Of Pterostilbene, And Its Human Pharmacokinetic Prediction Using Physiologically Based Pharmacokinetic Modeling, Palak S. Phansalkar

Theses and Dissertations

This dissertation research aimed to determine the preclinical pharmacokinetics of pterostilbene in vitro and develop a physiologically based pharmacokinetic (PBPK) model to simulate the animal and human PK profiles for pterostilbene. The in vitro PK characteristics of pterostilbene, including Caco-2 cell permeability, plasma protein binding, and metabolic stability were determined using rat and human intestinal & hepatic S9 subcellular fractions. The in vitro metabolic parameters determined in rat subcellular fractions were extrapolated to in vivo values to predict the oral bioavailability (F_oral) in rats. The predicted F_oral was found to be 43.0 %, which is similar to …

Carborane-Appended Levodopa For Prostate-Specific Membrane Antigen And Boron Neutron Capture Therapy, Alejandra Macias

Graduate Research Theses & Dissertations

A multi-step organic synthetic method to attach carborane cages to biocompatible organic compounds for application in Boron Neutron Capture Therapy (BNCT) was conducted. Boron Neutron Capture Therapy is a form of radiation therapy that promises a new and effective form of cancer treatment that involves the destruction of tumor cells using an isotope of boron (10B). The effectiveness of this therapy depends on the boron content accumulated in cancerous tissue. Carboranes are boron-rich clusters that possess unique physical and chemical properties making them suitable for a wide range of applications including medicinal chemistry. Because of their high boron content, carborane-appended …

Comparison Of Standard Dose Vs Higher Dose Low Molecular Weight Heparin And Unfractionated Heparin For Dvt Prophylaxis In Covid-19 Patients, Zachary Brown Pharmd, Munyaradzi Chakabva Md, Tabinda Akhtar Md, Sarah Polina Pharmd, Bcps, Michael Todt Pharmd, Bcccp, Mckenna Brauner Np, Meghan Mcguire Np, Aws Polina Md

Parkview Pharmacy Department

2021 ASHP Midyear Clinical Meeting / Virtual / Poster # 1121810

Objective: To evaluate whether higher dose VTE prophylaxis results in a significant reduction in VTE events without significant increase in bleeding risk when compared to standard dose VTE prophylaxis in patients with COVID-19 infection

Indomethacin Suppository Medication Use Evaluation For Prevention Of Post-Endoscopic Retrograde Cholangiopancreatography Pancreatitis (Pep) In A Community Health System, Caitlyn Patton, Jamie Gaul Pharmd, Bcps, Jennifer Sposito Pharmd

Parkview Pharmacy Department

2021 ASHP Midyear Clinical Meeting / Virtual / Poster

Objective: To evaluate the use and characterize the patient population receiving indomethacin suppositories for PEP with the goal of ensuring appropriate use.

Assessing Consistency With Standards Of Care Recommendations For Patients With Type 2 Diabetes At Hospital Discharge, Katherine Brown Pharmd, Timothy L. Johnston Bpharm, Pharmd, Bcps, Bcccp, Andrea Sloat Pharmd

Parkview Pharmacy Department

2021 ASHP Midyear Clinical Meeting / Poster ID#1129514

Objective: To assess the number of patients prescribed diabetic regimens consistent with American Diabetes Association (ADA) Standards of Care recommendations upon hospital discharge.

Implementation And Outcomes Assessment Of A 4-Factor Prothrombin Complex Concentrate (4f-Pcc) Tiered Fixed Dosing Protocol In A Health System., Mark Botros Bs, Jamie Gaul Pharmd, Bcps, Dana Lorber Pharmd, Bcps, Sarah Koontz Pharmd, Bcps

Parkview Pharmacy Department

2021 ASHP Midyear Clinical Meeting / Poster

The aim of this study was to evaluate compliance to the newly implemented fixed dosing protocol while assessing for the need for repeat doses of 4F-PCC and the need for further invasive intervention to reverse the bleeding.

Evaluation Of Intravenous Immunoglobulin (Ivig) Utilization After The Implementation Of Indication And Dosing Protocol At A Community Health System., Nicholas Buttermore, Jamie Gaul Pharmd, Bcps, Kate Oetting

Parkview Pharmacy Department

2021 ASHP Midyear Clinical Meeting / Poster

Objective: This study was conducted to evaluate the patient landscape of IVIG use within our community health system, both before and after protocol implementation. Our goals were to identify potential changes with the indication for use along with any prescribed dosing decreases while under protocol.

Evaluation Of Calcitonin Use For Hypercalcemia At Parkview Health, Alison Rydell, Jesse Christman Pharmd, Bcop, Jamie Gaul Pharmd, Bcps

Parkview Pharmacy Department

2021 ASHP Midyear Clinical Meeting / Poster

Objective: Evaluate the appropriateness of calcitonin use for hypercalcemia at Parkview Health, a not-for-profit community hospital health system.

Evaluation Of Emergency Department Treatment Of Pediatric Asthma Exacerbations, Paige Walker Pharmd, Amber Ooley Pharmd, Bcps, Bcccp, Kassandra Foellinger Pharmd, Bcps

Parkview Pharmacy Department

Objective:

Primary: to assess the appropriateness of medications ordered in the emergency department (ED).

Secondary: to assess the appropriateness of discharge medications and new encounters within 30 days for another asthma-related problem.

Analysis Of Hba1c, Medication Compliance, Income Subsidies, And Comorbidity In Medicare Type 2 Diabetics, Robert E. Lazarchik

Walden Dissertations and Doctoral Studies

Diabetes is one of America’s leading chronic diseases with comorbidities contributing to lower health statuses and increased health care costs. While it is known that lowering HbA1c reduces the deleterious effects of diabetes, the capability to identify people with diabetes at risk for uncontrolled HbA1c levels or developing comorbidities based on the compliance rates for different oral antihyperglycemic medication classes (OAMCs) and financial assistance programs does not yet exist. These quantitative longitudinal retrospective studies examined the association between medication compliance, using Proportion of Days Covered (PDC), by OAMC and Medicare financial aid programs, on predicting HbA1c levels and comorbidities in …

Translationally-Transformed Coupled-Cluster Theory For Periodic Systems, Boris Daniel Gutierrez-Cortes

University of the Pacific Theses and Dissertations

There are a lot of interesting problems in surface chemistry where quantum chemistry could give great insight, like reaction mechanisms in heterogeneous catalysis, the effect of surface functionalization on semiconductors, or the influence of defects on the reactivity of crystal surfaces.

Plane wave based methods applied to crystals cannot handle problems that are localized in nature like surface defects and adsorbates. On the other hand, molecular electronic structure techniques, which describe these effects and the locality of the electronic correlation well, are too computationally expensive to use on these systems.

In this work, we introduce translationally-transformed coupled-cluster (TT-CC) theory, a …

Investigating The Specificity Of Coiled-Coil Recognition, Melina Huey

University of the Pacific Theses and Dissertations

The bZIP transcription factors make up a family of long α-helical proteins that dimerize based on a pattern of hydrophobic residues and bind to DNA through a region of basic residues. Because binding specificity is a particular topic of interest, the dimerization interaction is attractive as a possible candidate to better understand protein quaternary structure. Use of the Knob-Socket (KS) model for determination of packing structure provides a novel approach to analyze protein-protein interactions. A KS analysis of the protein-protein interface provides unique insight into the specificity of the classical leucine zipper pseudo-7mer repeat. From an analysis of the KS …

In Vitro Comparative Study Of The Binding Affinity And Targeted-Drug Delivery Efficiency Of Egfr-Targeting Peptides, Jingda Wang

University of the Pacific Theses and Dissertations

Peptides have been used as targeting ligands in targeted drug delivery. Conjugating peptides to cytotoxicity agents via a linker to build peptide-drug conjugate (PDC) is a promising targeting strategy. The binding affinity of the peptide ligand and the receptor plays a crucial role in the PDC targeted drug delivery. Although the ligand binding which can be used in targeted drug delivery has been established conceptually, the quantitative or semi-quantitative contribution of binding affinity in targeting efficiency has not been fully explored. The optimal range of binding affinity of the peptide for targeted delivery remains unknown. Therefore, there is a lack …

Development Of Linked-Domain Protein Inhibitors Of The E2-Conjugating Enzyme Ube2d, Anneroos E. Nederstigt

University of the Pacific Theses and Dissertations

In most eukaryotic organisms, the ubiquitination pathway is one of the most important and versatile signaling systems in use. It is integral to processes such as protein degradation and homeostasis, DNA repair cell cycle regulation, signaling and regulation, epigenetics, and many more. Ubiquitin (Ub) is a short polypeptide of 8.6 kDa, 76 residues that functions as a reversible post-translation modification (PTM). It furthermore contains 7 different lysine residues (K6, K11, K27, K29, K33, K48, K63), all of which can form isopeptide linkages with one another to link individual Ub moieties to form unique polyUb chains onto substrates. The type of …

Bayesian-Derived Vancomycin Auc24h Threshold For Nephrotoxicity In Special Populations, Dan Ho

University of the Pacific Theses and Dissertations

A Bayesian-derived 24-hour area under the concentration-time curve over minimum inhibitory concentration from broth microdilution (AUC24h/MICBMD) ratio of 400 to 600 is recommended as the new monitoring parameter for vancomycin to optimize efficacy and minimize nephrotoxicity. The AUC24h threshold of 600 mg*h/L for nephrotoxicity was extrapolated from studies that assessed the general population. It is unclear if this upper threshold is consistent or varies when used in special populations such as critically ill patients, obese patients, patients with preexisting renal disease, and patients on concomitant nephrotoxins.The purpose of this study is to investigate the generalizability of the proposed vancomycin AUC24h …

Harnessing Transthyretin To Enhance The In Vivo Half-Life Of Human Interleukin-2 (Il-2), Fang Liu

University of the Pacific Theses and Dissertations

Protein therapeutics are available as cytokines, clotting factors, enzymes, hormones, growth factors, antibodies et al. They have been shown to be effective in treating a variety of important human diseases. Since human insulin was approved as the first recombinant protein therapeutic, this field has experienced rapid growth. One of the biggest challenges for protein therapeutics in clinical application is their short half-life. Except for monoclonal antibodies, which have serum half-life for weeks, most of the protein therapeutics have half-lives ranging from minutes to hours. Kidney filtration, proteasome degradation and liver metabolism are the main factors that attribute to their short …

Development Of Miriplatin-Loaded Nanoparticles Against Non-Small Cell Lung Cancer, Zhongyue Yuan

University of the Pacific Theses and Dissertations

Lung cancer claims the highest mortality and the second-most estimated new cases among all oncological diseases [1]. Non-small cell lung cancer (NSCLC) accounts for approximately 85% of all newly diagnosed lung cancers [2]. Approximately 40% of newly diagnosed lung cancer patients are stage IV. For stage IIIB/IV NSCLC, cytotoxic combination chemotherapy is standard first-line chemotherapy. A regimen of platinum (cisplatin or carboplatin) plus paclitaxel, gemcitabine, docetaxel, vinorelbine, irinotecan, or pemetrexed is the recommended clinical treatment [3].

Cisplatin is the first-generation platinum-based anti-cancer drug. Although cisplatin is much more effective than other platinum drugs at the same dosage [4], accumulating reports …

Impact Of 17-Beta Estradiol And Moderate-Intensity Exercise On Mesenteric Arterial Function Of Uc Davis Type-2 Diabetes Mellitus Rats, Md Rahatullah Razan

University of the Pacific Theses and Dissertations

The studies in this dissertation were designed to investigate the impacts of estrogen (17-β estradiol/E2) and moderate-intensity exercise (MIE) on the mesenteric arterial (MA) function of the University of California Davis type-2 diabetes mellitus (UCD-T2DM) Rat model. Our recent report suggests that diabetes impairs MA vasorelaxation in both sexes of the UCD-T2DM model. Particularly, we reported that MA from prediabetic male rats showed a greater impairment compared to that in prediabetic females. However, when females become diabetic, they exhibit a greater vascular dysfunction than males. Therefore, the aim of the first study was to investigate whether female sex hormone, specifically …

Development Of Asiatic Acid-Loaded Solid Lipid Nanoparticles For Nose To Brain Delivery: Ex Vivo And In Vivo Evaluation, Tissana Rojanaratha

Chulalongkorn University Theses and Dissertations (Chula ETD)

Asiatic acid (AA) has neuroprotective properties potential for prevention and treatment of Alzheimer’s disease. Natural waxes with various ratios of tween 80 and span 80 or soybean lecithin were formulated to obtain AA-loaded solid lipid nanoparticles (AA-SLN) in order to improve direct nose to brain transport. Optimal AA-SLN had particle size below 200 nm with uniform size distribution as confirmed by transmission electron microscope (TEM) results and zeta potential of nearly -30 mV indicating low risk on particle aggregation. Formulation with rice bran wax, tween80 and soybean lecithin (AA-RwS100) showed the highest drug content and entrapment efficiency of >98%. Differential …

Melanogenesis-Stimulating Isoflavonoids From Dalbergia Parviflora, Ponsawan Netcharoensirisuk

Chulalongkorn University Theses and Dissertations (Chula ETD)

Melanin, a cellular pigment, found in skin, hair and eyes is produced by melanocytes located at the basal layer of epidermis. Abnormal melanin production causes various pigmentation diseases. Low melanin content or dysfunction of melanogenesis not only decreases self-confidence and the quality of life but also enhances skin damage by external stimuli. Until now, there has been no reported remedy that possesses high efficacy and safety profile for hypopigmentation treatment in human. Development of novel darkening compounds from natural resources has recently become of an interest. In this study, the potent melanin-stimulating agents were screened from 45 compounds isolated from …

Study Of Self-Assembled Hyaluronic Acid Based Nanogel As Solubilization And Stabilization System For Asiatic Acid, Yu Yu Win

Chulalongkorn University Theses and Dissertations (Chula ETD)

Asiatic acid (AA), natural triterpene found in Centalla asiatica, possesses polypharmacological properties that can contribute to the treatment and prophylaxis of various diseases. However, its hydrophobic nature and rapid metabolic rate lead to poor bioavailability. The aim of this research is to study nanogel system from hyaluronic acid (HA) for solubilization and stability enhancement of AA. Poly(N-isopropylacrylamide) (pNIPAM) was conjugated onto HA using carbodiimide reaction followed by 1H NMR characterization. pNIPAM-grafted HA (HA-pNIPAM) nanogels were prepared with three concentrations of polymer, 0.1%, 0.15% and 0.25% w/v in water by sonication method. AA was loaded into the nanogel by incubation method. …

Development Of Tetrahydrocurcumin-Loaded Chitosan-Coated-Nanostructured Lipid Carriers For Topical Treatment Of Psoriasis, Hoang Thien Truong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chitosan (Ch)-coated nanostructured lipid carriers (NLCs) have great potential for topical delivery with high localization of chemotherapeutics. In this study, tetrahydrocurcumin (THC), a primary metabolite of curcumin with enhanced antioxidant and anticancer properties, was used as a model drug to prepare NLCs. Response surface methodology was employed to optimize THC-loaded Ch-coated NLCs (THC-Ch-NLCs) fabricated by high-shear homogenization. The optimized THC-Ch-NLCs had particle size of 244 ± 18 nm, zeta potential of -17.5 ± 0.5 mV, entrapment efficiency of 76.6 ± 0.2% and drug loading of 0.28 ± 0.01 mg/mL. In vitro release study of THC-Ch-NLCs showed sustained release following the …

Development Of Hek-293t Cells Stably Expressing The C-X-C Chemokine Receptor Type 4 (Cxcr4) For Binding Assay Of Potential Anti-Breast Cancer Agents, Thi Thai Ha Dinh

Chulalongkorn University Theses and Dissertations (Chula ETD)

The C-X-C chemokine receptor type 4 (CXCR4) has been recognized as a potential target in cancer drug discovery based on the high expression in cancer cells, particularly invasive breast cancer. This study aims to construct CXCR4-overexpressed cells to screen new anti-breast cancer agents targeting CXCR4 and identify the mediated target for anticancer effect. Firstly, five genetic isotypes of CXCR4, namely A, B, C, D and E, were amplified from Jurkat T cells. Amplicons of each CXCR4 isotype were ligated into cloning vectors and subcloned into expression vectors. They were transformed into competent Escherichia coli strain DH5-alpha cells. The presence of …

Spray Dried Asiatic Acid-Palm Oil Nanoemulsion With Improved Characteristics And Antihypertensive Effect Study In Rat Model Of Hypertension, Aditya Trias Pradana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hypertension is a risk factor for premature death worldwide.It is characterized by an increase in blood pressure, so it needs therapy to regulate it and keep the value normal. Asiatic acid (AA) and palm oil (PO) were alternative antihypertensive agents that were promising to be developed. Both were natural compounds obtained from the Centella asiatica and Elaesis guineensis plants. However, low solubility, low absorption, rapid metabolism, low bioavailability and high oxidation were the limitation for both of them as an active compounds. Thus, a combination of nanoemulsion formulation and drying with a spray dryer was necessary to improve the physicochemical …