Medicine and Health Sciences Commons^™

Early Life Exposure To Broccoli Sprouts Confers Stronger Protection Against Enterocolitis Development In An Immunological Mouse Model Of Inflammatory Bowel Disease, Lola Holcomb, Johanna M. Holman, Molly Hurd, Brigitte Lavoie, Louisa Colucci, Benjamin Hunt, Timothy Hunt, Marissa Kinney, Jahnavi Pathak, Gary M. Mawe, Peter L. Moses, Emma Perry, Allesandra Stratigakis, Tao Zhang, Grace Chen, Suzanne L. Ishaq, Yanyan Li

Pharmacy Faculty Scholarship

Crohn’s disease (CD) is a presentation of inflammatory bowel disease (IBD) that manifests in childhood and adolescence and involves chronic and severe enterocolitis, immune and gut microbial dysregulation, and other complications. Diet and gut-microbiota-produced metabolites are sources of anti-inflammatories that could ameliorate symptoms. However, questions remain on how IBD influences biogeographic patterns of microbial location and function in the gut, how early life transitional gut communities are affected by IBD and diet interventions, and how disruption to biogeography alters disease mediation by diet components or microbial metabolites. Many studies on diet and IBD use a chemically induced ulcerative colitis model, …

Brain Microvascular Endothelial Cells Possess A Second Cilium That Arises From The Daughter Centriole, Karthikeyan Thirugnanam, Ankan Gupta, Francisco Nunez, Shubhangi Prabhudesai, Amy Y. Pan, Surya M. Nauli, Ramani Ramchandran

Pharmacy Faculty Articles and Research

Primary cilia from the brain microvascular endothelial cells (ECs) are specialized cell-surface organelles involved in mediating sensory perception, cell signaling, and vascular stability. Immunofluorescence (IF) analysis of human primary brain microvascular ECs reveals two cilia per cell. To confirm the in vitro observation of the two-cilia phenotype in human primary brain ECs, ECs isolated from mouse brain were cultured and stained for cilium. Indeed, brain ECs from a ciliopathic mouse (polycystic kidney disease or Pkd2−/−) also possess more than one cilium. Primary cilium emerges from the mother centriole. Centriole analysis by IF suggests that in brain ECs, markers …

Repurposing Egfr Inhibitors For Oral Cancer Pain And Opioid Tolerance, Maria Daniela Santi, Morgan Zhang, Naijiang Liu, Chi T Viet, Tongxin Xie, Dane D Jensen, Moran Amit, Huilin Pan, Yi Ye

Faculty, Staff and Student Publications

Oral cancer pain remains a significant public health concern. Despite the development of improved treatments, pain continues to be a debilitating clinical feature of the disease, leading to reduced oral mobility and diminished quality of life. Opioids are the gold standard treatment for moderate-to-severe oral cancer pain; however, chronic opioid administration leads to hyperalgesia, tolerance, and dependence. The aim of this review is to present accumulating evidence that epidermal growth factor receptor (EGFR) signaling, often dysregulated in cancer, is also an emerging signaling pathway critically involved in pain and opioid tolerance. We presented preclinical and clinical data to demonstrate how …

Interprofessional Health Fairs: An Effective Tool For Interprofessional Education?, Caleb Holloway, Ashley Barbo, Bryan Donald, Min Jee Kim

Journal of Interprofessional Practice and Collaboration

Purpose: The purpose of this study was to determine whether interprofessional health fairs have a significant impact on the interprofessional education of students in health care disciplines.

Methods: An optional survey was administered to students participating in the health fairs in 2021 and 2022. The survey utilized four retrospective pre and post ICCAS assessment questions to address IPEC core competencies and two open ended questions to provide feedback about the health fair and report anything new learned from or about another health care discipline. The self-assessment questions were reported on a 5-point Likert scale and recorded in aggregate for both …

Cgm Use With Interprofessional Therapy Management Improves Hba1c Levels In T2dm Patients, Stephen R. Hill, Courtney Robertson, Teri B. O'Neal, Sonia Garner

Journal of Interprofessional Practice and Collaboration

Background

Type 2 Diabetes Mellitus (T2DM) is a chronic disease state with increased complications over time from uncontrolled glucose. Significant data has shown benefits of continuous glucose monitoring (CGM) in reducing glycated hemoglobin (HbA1c) levels of Type 1 Diabetes Mellitus (T1DM) patients, but a lack of robust evidence is seen in T2DM. This study assessed the impact of short-term CGM placement on HbA1c levels in T2DM.

Objectives

The primary outcome was the change in baseline HbA1c levels in patients with T2DM up to six months after CGM placement. Secondary outcomes assessed intensification of diabetes medications made by an interprofessional team …

Diabetes-Associated Hyperglycemia Causes Rapid-Onset Ocular Surface Damage, Judy Weng, Christopher Ross, Jacob Baker, Saleh Alfuraih, Kiumars Shamloo, Ajay Sharma

Pharmacy Faculty Articles and Research

Purpose: The metabolic alterations due to chronic hyperglycemia are well-known to cause diabetes-associated complications. Short-term hyperglycemia has also been shown to cause many acute changes, including hemodynamic alterations and osmotic, oxidative, and inflammatory stress. The present study was designed to investigate whether diabetes-associated hyperglycemia can cause rapid-onset detrimental effects on the tear film, goblet cells, and glycocalyx and can lead to activation of an inflammatory cascade or cellular stress response in the cornea.

Methods: Mouse models of type 1 and type 2 diabetes were used. Tear film volume, goblet cell number, and corneal glycocalyx area were measured on …

Quantification Of Observable Behaviors Following Oral Administration Of Oxycodone And Nalfurafine In Male Rhesus Monkeys, Sally L. Huskinson, Donna M. Platt, Zachary R. Smith, William S. Doyle, C. Austin Zamarripa, Kristen Dunaway, Thomas E. Prisinzano, Kevin B. Freeman

Pharmaceutical Sciences Faculty Publications

BACKGROUND: Recent preclinical studies have investigated the atypical kappa-opioid receptor (KOR) agonist, nalfurafine, as a co-formulary with mu-opioid receptor (MOR) agonists as a potential deterrent for misuse. However, no study has investigated effects of nalfurafine combined with a MOR agonist using an oral route of administration. The objective of the current study was to measure behavioral effects of orally administered oxycodone and nalfurafine, alone and combined, in rhesus monkeys using a quantitative behavioral observation procedure.

METHODS: Adult male rhesus monkeys (N=5) were orally administered vehicle, oxycodone (0.56-1.8mg/kg), nalfurafine (0.001-0.0056mg/kg), or mixtures (1.0mg/kg oxycodone/0.001-0.0056mg/kg nalfurafine) in a Jell-O vehicle at multiple …

Demonstrating The Benefits Of Antihypertensive Nighttime Dosing And Indapamide Usage In Hypertension Management, Jonathon S. Newsome

Pharmacy Faculty Publications and Presentations

Background: Uncontrolled hypertension, specifically nocturnal hypertension, increases the risk for significant clinical outcomes. Evidence on the use of nighttime antihypertensives is scant and conflicting. In addition, hydrochlorothiazide continues to be the primary thiazide used despite being the least potent. Objective: The primary purpose of this study was to evaluate instituting nighttime dosing to control hypertension and compare the short-term effectiveness of blood pressure control with indapamide versus hydrochlorothiazide. Methods: This was a retrospective, observational study. Participant inclusion criteria consisted of patients 18 years of age or older, a current diagnosis of hypertension, and hypertension that required medical therapy. The investigator …

Tigit Contributes To The Regulation Of 4-1bb And Does Not Define Nk Cell Dysfunction In Glioblastoma, Kyle B. Lupo, Sandra Torregrosa-Allen, Bennett D. Elzey, Sagar Utturkar, Nadia A. Lanman, Aaron A. Cohen-Gadol, Veronika Silvova, Mackenzie Mcintosh, Karen E. Pollok, Sandro Matosevic

Purdue University Libraries Open Access Publishing Fund

TIGIT is a receptor on human natural killer (NK) cells. Here, we report that TIGIT does not spontaneously induce inhibition of NK cells in glioblastoma (GBM), but rather acts as a decoy-like receptor, by usurping binding partners and regulating expression of NK activating ligands and receptors. Our data show that in GBM patients, one of the underpinnings of unresponsiveness to TIGIT blockade is that by targeting TIGIT, NK cells do not lose an inhibitory signal, but gains the potential for new interactions with other, shared, TIGIT ligands. Therefore, TIGIT does not define NK cell dysfunction in GBM. Further, in GBM, …

The Effect Of An Electric Current On Human Body (A Review), Yasir Khaleel Almusawi, Maha Raad Hashim Al-Sammarraie, Mukaram D. Shikara

Al-Esraa University College Journal for Medical Sciences

According to the World Health Organization (WHO), four people are killed every week by electrocution in the last decade, while 3% of people (suffering from electrical injuries) were admitted to emergency wards. The severity of electric injury depends on the electrical resistance of the human body, which depends on several factors such as the long duration of electric contact, type of the current (AC or DC), intensity of the current (amperes/voltages), sites of entry and exit points, wet or dry body, individual metabolism of the person, sweat gland activity, human body mass, type of tissue it travels through and its …

Biological Activity Of Rats Treated With Riboflavin In Polymeric Plga As Injectable Form, Sarah A. Hamood, Jabar A. Faraj, Ziad T. Al-Dahan

Al-Esraa University College Journal for Medical Sciences

The current study aims to optimize a fast, reproducible, reliable, and effective technique for estimating the biological activity of Vitamin B2 in injectable dose form using reversed-phase high-performance liquid chromatography (RP-HPLC). A biodegradable polymer, PLGA (Poly (lactic-co-glycolic acid) 50:50 type, approved as an active ingredient for parenteral administration, was used to load the vitamin in the injection volume of 1mg. Rats, both male and female, were employed as the animal models. Each animal was hosted for a duration of 40 days, and the activity of vitamin B2 was monitored at rapid intervals over six weeks using an HPLC device on …

Analysis Of Chloramphenicol, Sulphamethoxazole, And Sulfanilamide Drugs By Reversed-Phase High-Performance Liquid Chromatography With Fluorescent Detector, Tariq Y. Mahmoud, Aziz L. Jarallah, Isam S. Hamza

Al-Esraa University College Journal for Medical Sciences

This study proposed to analyze chloramphenicol (CPA), sulfamethoxazole (SMX), and sulfanilamide (SNA) drugs by reverse-phase liquid chromatography method in the form of pharmaceutical preparations and synthetic mixture. For this method, column C18-ODS (25 cm x 4.6 mm) was used with an isocratic elution mobile phase composed of methanol and 2% orthophosphoric acid, fluorescent detector (Ex= 310 nm, Em= 440 nm). It was found that the time of retention for CPA, SMX, and SNA was 8.99 ± 0.02, 10.76 ± 0.2, and 8.21 ± 0.2 min, respectively. The suggested method has been validated for limit of detection, limit of quantitation, linearity, …

Hyaluronic Acid Gel Effectiveness As An Adjunctive Treatment In Patients With Perio Dontal Disease, Ban Zuhair Ahmed

Al-Esraa University College Journal for Medical Sciences

Background: Both hard and soft periodontal components require hyaluronic acid (HA) for extracellular matrices to function properly. HA is crucial to understanding how inflammation and wound healing work. Different amounts of HA are found in periodontal tissues, including non-mineralized tissues like gingiva and periodontal ligament, and lesser levels in mineralized tissues like cementum and alveolar bone. Preliminary research indicates that HA can control periodontal tissue regeneration and treat periodontal disease. In both deeper periodontal tissues and marginal gingiva, HA helps to relieve symptoms. Aims: This research aimed to detect the influence of hyaluronic acid 1% gel as an adjunctive therapy …

Influence Of Il-1ss, Anti-Cdtb And Histamine In Irritable Bowel Syndrome, Khalid Mahdi Salih, Ola Amer Jasim

Al-Esraa University College Journal for Medical Sciences

Irritable Bowel Syndrome (IBS) is a common gut disorder that affects approximately 11% of the global population and negatively affects the quality of life of patients and imposes a significant socioeconomic burden. A control-based study was conducted on 42 Iraqi patients with IBS who presented at private clinics for gastrointestinal diseases (GIDs), along with 20 healthy individuals matched in age and gender to act as a control group. The study aimed to determine the frequency of IBS among Iraqi patients, as well as the influence of biomarkers on the disease's initiation, type, and severity such as IL-1ß, anti-Cytolethal distending toxin …

The Relationship Between The Nervous System And Dna Expression(A Review), Maha Raad Hashim Al-Sammarraie, Yasir Khaleel Almusawi, Mukaram D. Shikara

Al-Esraa University College Journal for Medical Sciences

Neurotransmitters travel through synapses mediated by neurons. The DNA inside the neurons does not replicate since it arrests in the G1 phase of the cell cycle, which explains the absence of replication as DNA replication occurs during the S phase, which is preceded by the G1 phase. Experiments have shown that environmental factors can influence the DNA code and have been tried in both plant and animal kingdoms over the last two centuries with great success, leading to the belief that environmental factors can prompt the brain to send signals (neurotransmitters) to somatic cells. The next question will be: "Is …

Liposome Synthesis And Evaluation In The Hek-293 Cell Line, Christine Skibinski

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Liposomes were synthesized using the thin film method. A lyophilized power of egg-derived phosphatidylcholine, stearylamine, and cholesterol were added to ethanol and dried under argon to form a lipid cake. The lipid cake was rehydrated with dPBS and sonicated at 60°C forming a heterogenous batch of liposomes. Our results revealed the average size of the liposomes, determined by Dynamic Light Scattering, was approximately 223.1nm, while demonstrating a weakly positive zeta-potential of 1.9± 8.07mv. Next, we tested the antitumor action of the liposomes in the HEK-293 cell line via an MTT assay. We observed that the liposomes were able to inhibit …

Tolfenamic Acid Derivatives: A New Class Of Transcriptional Modulators With Potential Therapeutic Applications For Alzheimer’S Disease And Related Disorders, Juanetta Hill, Karim E. Shalaby, Syed W. Bihaqi, Bothaina H. Alansi, Benjamin Barlock, Keykavous Parang, Richard Thompson, Khalid Ourarhni, Nasser H. Zawia

Pharmacy Faculty Articles and Research

The field of Alzheimer’s disease (AD) has witnessed recent breakthroughs in the development of disease-modifying biologics and diagnostic markers. While immunotherapeutic interventions have provided much-awaited solutions, nucleic acid-based tools represent other avenues of intervention; however, these approaches are costly and invasive, and they have serious side effects. Previously, we have shown in AD animal models that tolfenamic acid (TA) can lower the expression of AD-related genes and their products and subsequently reduce pathological burden and improve cognition. Using TA as a scaffold and the zinc finger domain of SP1 as a pharmacophore, we developed safer and more potent brain-penetrating analogs …

Untargeted Metabolomics Analysis On Kidney Tissues From Mice Reveals Potential Hypoxia Biomarkers, Muhammad Imran Sajid, Francisco J. Nunez, Farideh Amirrad, Moom Rahman Roosan, Tom Vojtko, Scott Mcculloch, Amal Alachkar, Surya M. Nauli

Pharmacy Faculty Articles and Research

Chronic hypoxia may have a huge impact on the cardiovascular and renal systems. Advancements in microscopy, metabolomics, and bioinformatics provide opportunities to identify new biomarkers. In this study, we aimed at elucidating the metabolic alterations in kidney tissues induced by chronic hypoxia using untargeted metabolomic analyses. Reverse phase ultrahigh performance liquid chromatography-mass spectroscopy/mass spectroscopy (RP–UPLC–MS/MS) and hydrophilic interaction liquid chromatography (HILIC)–UPLC–MS/MS methods with positive and negative ion mode electrospray ionization were used for metabolic profiling. The metabolomic profiling revealed an increase in metabolites related to carnitine synthesis and purine metabolism. Additionally, there was a notable increase in bilirubin. Heme, N-acetyl-L-aspartic …

Addressing The Crisis: Leveraging The United Nations Sustainable Development Goals To Prepare Student Leaders To Tackle The Opioid Epidemic, Sharon Xavioer, Laressa Bethishou, Madeline Dintzner, Reza Taheri, Jelena Lewis

Pharmacy Faculty Articles and Research

The United States faces several ongoing public health issues including the opioid epidemic. This article describes a new model aimed at providing a framework that incorporates the United Nations (UN) Sustainable Development Goals (SDGs) to develop pharmacy student leaders through education, experiences, and development of critical skills. This holistic approach can serve as an example methodology to equip future leaders across public health domains to tackle many of the critical problems we face today.

Resisting The Resistance: How The Environment Is Impacting Antibiotic Resistance, Hannah Hinrichs

BUHealth

Upon their discovery, antibiotics were known as “wonder drugs” due to their ability to cure serious bacterial infections. While antibiotics are still revolutionary in healthcare today, the emergence of antibiotic resistance is a growing threat to public health. Antibiotic resistance is simply a normal adaptive response where bacteria evolve over time. However, overuse of antibiotics, sub-optimal treatment regimens, and environmental exposures create optimal conditions for antibiotic resistance to develop and spread. Common ways antibiotics enter the environment are via wastewater and agricultural use. Even with properly functioning wastewater treatment systems, full removal of resistant bacteria may not be possible as …

You Are What You Eat, Allyson Ditmars

BUHealth

The gut microbiome is the wide array of good and bad bacteria found in the digestive tract of all humans. Although the composition of gut microbiota is developed before someone is even born, there are nutritional decisions made in adulthood that can impact the health of the gut. Certain nutritional foods can increase the diversity of the gut and create a beneficial environment. On the other hand, many foods found in the common American diet can negatively impact the microbiome. The gut can suffer from a poor diet when the permeability of the intestinal barrier and the diversity richness is …

Locked Up: A Look Into Healthcare Behind Bars, Ella Davis

BUHealth

The United States incarceration rate is the highest in the world. Incarcerated individuals are guaranteed a right to healthcare but, it falls short in many correctional facilities, as systemic barriers and inadequate funding often result in substandard care and poor health outcomes. There are multiple challenges that come with providing healthcare in prisons such as limited resources, lack of funding, and staff shortages. Prisoners at every level of the correctional system are known to be less healthy than the general population, and despite multiple efforts to improve healthcare, many prisoners still receive inadequate healthcare. Improving prison healthcare and increasing funding …

Rosuvastatin Calcium And Tissue Engineering; An Introductory Overview, Islam M. Adel, Mohamed F. Elmeligy, Nermeen A. Elkasabgy

Bulletin of Faculty of Pharmacy Cairo University

All tissues are subjected to the risk of tissue damage. Tissue damage refers to any alteration to the structure and/or the function of that tissue. To counter tissue damage, our bodies are equipped with a self-healing mechanism aiming at restoring tissue structure and function. The underlying mechanism, however, is rather poor and slow in mammalians. Moreover, in cases of severe damage, the mechanism is insufficient to repair the damage and usually, surgical intervention is mandated. For that and more, tissue engineering was conceptualized as a multidisciplinary science field in which an implantable scaffold, loaded with cells, biological cues and/or chemical …

Improving Pharmacist-Led Pediatric Patient Education On Oral Chemotherapy At Home, Anika Patel, Christopher M. Nguyen, Kristin Willins, Elsabella Y. Wang, Grace Magedman, Sun Yang

Pharmacy Faculty Articles and Research

Oral chemotherapy (OC) has been increasingly used in pediatric patients diagnosed with cancer, which is primarily managed in the outpatient setting. Different from adults, pediatric patients face unique challenges in administering these hazardous medications at home. Because of the complexity of pediatric pharmaceutical care and the hazardous nature of chemotherapy agents, comprehensive patient education is imperative to mitigate the potential safety risks associated with OC administration at home. Pharmacists play a vital role in patient education and medication consultations. However, the lack of practice guidelines and limited resources supporting OC counseling are noted. Additional barriers include insufficient knowledge and training …

Tertiary Drug Information Sources For Treatment And Prevention Of Covid-19., Robert D. Beckett, Yashawna Brattain, Judy Truong, Genevieve Engle

Parkview Pharmacy Department

OBJECTIVE: To evaluate tertiary drug information databases in terms of scope, consistency of content, and completeness of COVID-19 drug information.

METHODS: Five electronic drug information databases: Clinical Pharmacology, Lexi-Drugs, AHFS DI (American Hospital Formulary Service Drug Information), eFacts and Comparisons, and Micromedex In-Depth Answers, were evaluated in this cross-sectional evaluation study, with data gathered from October 2021 through February 2022. Two study investigators independently extracted data (parallel extraction) from each resource. Descriptive statistics were primarily used to evaluate scope (i.e., whether the resource addresses use of the medication for treatment or prevention of COVID-19) and completeness of content (i.e., whether …

Fall 2023 Edition, Southwestern Oklahoma State University

The Sig

The Sig Newsletter from the Southwestern Oklahoma State University College of Pharmacy - Fall 2023 Edition

Phad 780: General Linear Models, John P. Bentley

GMAS Course Syllabi

No abstract provided.

Sharksrx Newsletter Fall 2023, Nova Southeastern University

NSU Sharks Rx

No abstract provided.

September / October 2023, Randy Curry, Cindy Brooks

RURAL ROCKS

This is the September & October 2023 issue of Rural Rocks from the SWOSU College of Pharmacy.

Therapeutic Effects Of Combining Curcumin And Swimming In Osteoarthritis Using A Rat Model, Mona M Saber, Manal Moustafa Mahmoud, Hesham M Amin, Reham M Essam

Faculty, Staff and Student Publications

Osteoarthritis (OA) is a common debilitating degenerative disease of the elderly. We aimed to study the therapeutic effects of combining curcumin and swimming in monosodium iodoacetate (MIA)-induced OA in a rat model. The rats were divided into 5 groups (n = 9). Group 1 received saline and served as a control group. Groups 2-5 were injected intra-articularly in the right knee with 100 μL MIA. One week later, groups 3 and 5 were started on daily swimming sessions that gradually increased to 20-mins per session, and for groups 4 and 5, oral curcumin was administered at a dose of 200 …