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Articles 4831 - 4860 of 4999
Full-Text Articles in Medicine and Health Sciences
Mrnas Encoding Aquaporins Are Present During Murine Preimplantation Development., H Offenberg, L C Barcroft, A Caveney, D Viuff, P D Thomsen, A J Watson
Mrnas Encoding Aquaporins Are Present During Murine Preimplantation Development., H Offenberg, L C Barcroft, A Caveney, D Viuff, P D Thomsen, A J Watson
Obstetrics & Gynaecology Publications
The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular water channels that allow water to flow rapidly across plasma membranes in the direction of osmotic gradients. Ten different AQPs have been identified to date. Murine preimplantation stage embryos were flushed from the oviducts and uteri of superovulated CD1 mice. Reverse transcription-polymerase chain reaction (RT-PCR) methods employing primer sets designed to amplify conserved sequences of AQPs (1-9) …
Slouching Toward Barbarism--The Quest To Limit Partial Birth Abortion After Stenberg V. Carhart, Todd Goudy
Slouching Toward Barbarism--The Quest To Limit Partial Birth Abortion After Stenberg V. Carhart, Todd Goudy
West Virginia Law Review
No abstract provided.
Recognition Of Left Atrial Aneurysm By Fetal Echocardiography., R R. Fountain-Dommer, H B. Wiles, C O. Shuler, S M. Bradley, Girish S. Shirali
Recognition Of Left Atrial Aneurysm By Fetal Echocardiography., R R. Fountain-Dommer, H B. Wiles, C O. Shuler, S M. Bradley, Girish S. Shirali
Manuscripts, Articles, Book Chapters and Other Papers
No abstract provided.
Dna Ploidy Analysis Of Borderline Epithelial Ovarian Tumours, Saba Lodhi, Sabeen Najam, Shahid Pervez
Dna Ploidy Analysis Of Borderline Epithelial Ovarian Tumours, Saba Lodhi, Sabeen Najam, Shahid Pervez
Department of Pathology and Laboratory Medicine
Objective: Borderline epithelial ovarian tumors not uncommonly pose a great difficulty to surgical pathologists as morphologically they may show very similar features as those of malignant epithelial tumors except invasion. However it is important to separate these from their invasive counterparts because of their superior prognosis. Recently, attention has been focussed on the prognostic value of flow cytometric analysis of DNA ploidy in borderline epithelial ovarian tumors. The purpose of this study is to investigate whether flow cytometric analysis of cellular DNA content acts as a useful adjunct to the histopathological diagnosis of borderline malignancy.
Materials and Methods: Fifteen histologically …
Phosphonated Agents And Their Antiangiogenic And Antitumorigenic Use, Delwood C. Collins, Antonio Gagliardi, Peter Nickel
Phosphonated Agents And Their Antiangiogenic And Antitumorigenic Use, Delwood C. Collins, Antonio Gagliardi, Peter Nickel
Obstetrics and Genecology Faculty Patents
Phosphonic acid agents are synthesized and characterized which are potent inhibitors of angiogenesis, tumorigenesis and metalloproteinase activity. Their method of use for the inhibition of angiogenesis and metalloproteinase and the treatment of tumors is also shown.
Differential Involvement Of Na(+),K(+)-Atpase Isozymes In Preimplantation Development Of The Mouse., D J Macphee, D H Jones, K J Barr, D H Betts, A J Watson, G M Kidder
Differential Involvement Of Na(+),K(+)-Atpase Isozymes In Preimplantation Development Of The Mouse., D J Macphee, D H Jones, K J Barr, D H Betts, A J Watson, G M Kidder
Obstetrics & Gynaecology Publications
Na(+),K(+)-ATPase plays an essential role in mammalian blastocoel formation (cavitation) by driving trans-epithelial sodium transport. Previously, the alpha1 and beta1 subunit isoforms of this enzyme were identified in preimplantation mouse embryos and were assumed to be responsible for this function. Here we show that mRNAs encoding an additional alpha subunit isoform (alpha3) and the remaining two beta subunit isoforms are also present in preimplantation embryos. Whereas alpha3 mRNA accumulates between the four-cell and the blastocyst stages and thus results from embryonic transcription, the same could not be demonstrated for beta2 and beta3 mRNAs. Immunoblot analyses confirmed that these subunits are …
Uterine Atony At A Tertiary Care Hospital In Pakistan: A Risk Factor Analysis, S H. Feerasta, A Motiei, S Motiwala, N F. Zuberi
Uterine Atony At A Tertiary Care Hospital In Pakistan: A Risk Factor Analysis, S H. Feerasta, A Motiei, S Motiwala, N F. Zuberi
Department of Obstetrics & Gynaecology
Objectives: To identify risk factors for uterine atony following assisted or unassisted vaginal delivery.
Design: This hospital based case control study was done at The Aga Khan University Karachi, Pakistan. Cases were defined as all women with uterine atony within 24 hours of an assisted or unassisted vaginal delivery. Controls were based on women with normal assisted or unassisted vaginal delivery without uterine atony. Data abstracted form the medical records; adjusted odds ratios were estimated by multiple logistic regression.
Results: Factors having a significant association with uterine atony were gestational diabetes mellitus (odds ratio 7.6, 95% CI 6.9-9.0, p = …
Impact Of Bovine Oocyte Maturation Media On Oocyte Transcript Levels, Blastocyst Development, Cell Number, And Apoptosis., A J Watson, P De Sousa, A Caveney, L C Barcroft, D Natale, J Urquhart, M E Westhusin
Impact Of Bovine Oocyte Maturation Media On Oocyte Transcript Levels, Blastocyst Development, Cell Number, And Apoptosis., A J Watson, P De Sousa, A Caveney, L C Barcroft, D Natale, J Urquhart, M E Westhusin
Obstetrics & Gynaecology Publications
The objectives were 1) to investigate the effects of oocyte maturation in serum-free and amino acid-supplemented defined media on oocyte transcript levels, blastocyst cell number, and apoptosis; 2) to investigate the influence of oocyte maturation culture atmosphere on blastocyst development, total cell number, and apoptosis; and 3) to examine the influence of epidermal growth factor (EGF) during oocyte maturation on blastocyst cell number and apoptosis. The results demonstrate that blastocysts derived from in vitro maturation, fertilization, and embryo culture protocols undergo apoptosis but that apoptotic levels are not greatly influenced by the oocyte maturation environment. Amino acid supplementation of oocyte …
The Effect Of Grandmultiparity On Pregnancy Related Complications: The Aga Khan University Experience, S Munim, M H. Rahbar, M Rizvi, N Mushtaq
The Effect Of Grandmultiparity On Pregnancy Related Complications: The Aga Khan University Experience, S Munim, M H. Rahbar, M Rizvi, N Mushtaq
Department of Obstetrics & Gynaecology
Background: Grandmultiparity has been associated with complications for both mother and the fetus.Objective: To evaluate if grandmultiparity is a risk factor in the presence of adequate antenatal care.
Setting: A tertiary care teaching hospital.
Methods: It was a retrospective study conducted in the department of Obstetrics & Gynecology at The Aga Khan University Hospital in Karachi. During this period 9253 women were delivered, out of these 143 booked grandmultiparas (GMP) served as cases. The outcome of these women was compared with 430 non-grandmultiparas (NGMP). Logistic regression model was used to adjust for potential confounders.
Results: Grandmultiparas had almost three times …
Critical Analysis Of Interventions Against Fgc In Egypt, Nahla G. Abdel-Tawab, Sahar Hegazi
Critical Analysis Of Interventions Against Fgc In Egypt, Nahla G. Abdel-Tawab, Sahar Hegazi
Reproductive Health
Community-based programs designed to discourage the practice of female genital cutting (FGC) in Egypt started as early as the 1920s, however, NGOs seldom document the implementation, strengths, and weaknesses of each approach; or difficulties faced in implementation or ways of overcoming those difficulties. The present meta-assessment was designed by the Population Council to address these issues. Recommendations proposed to increase the effectiveness of anti-FGC interventions in Egypt include: conducting formative research to assess the needs of individuals/communities; using more participatory learning techniques in awareness-raising seminars and training workshops; focusing messages not only on the health hazards of FGC, but also …
Effects Of Superovulated Heifer Diet Type And Quantity On Relative Mrna Abundances And Pyruvate Metabolism In Recovered Embryos., C Wrenzycki, P De Sousa, E W Overström, R T Duby, D Herrmann, A J Watson, H Niemann, D O'Callaghan, M P Boland
Effects Of Superovulated Heifer Diet Type And Quantity On Relative Mrna Abundances And Pyruvate Metabolism In Recovered Embryos., C Wrenzycki, P De Sousa, E W Overström, R T Duby, D Herrmann, A J Watson, H Niemann, D O'Callaghan, M P Boland
Obstetrics & Gynaecology Publications
This study investigated the effects of quantity and type of diet fed to superovulated donor heifers on molecular and metabolic indices of embryonic development. These effects included the relative abundances of mRNAs for the alpha 1 subunit of Na/K-ATPase and the antioxidant enzyme Cu/Zn-SOD, as well as pyruvate utilization in bovine morulae and blastocysts developed in vivo. Heifers were fed a daily ration of either grass silage and a citrus-beet pulp-based concentrate or grass silage and a barley-based concentrate for 116 days, both at 3 kg per day or ad libitum. In embryos derived from heifers fed the pulp-based diets, …
Intimate Partner Violence And Cervical Neoplasia, Ann L. Coker, Maureen Sanderson, Mary Kay Fadden, Lucia Pirisi
Intimate Partner Violence And Cervical Neoplasia, Ann L. Coker, Maureen Sanderson, Mary Kay Fadden, Lucia Pirisi
CRVAW Faculty Journal Articles
Intimate partner violence (IPV) is associated with a range of adverse physical health outcomes, including chronic and infectious diseases. An emerging literature suggests that partner violence and specifically sexual violence may be associated with an increased risk of cervical neoplasia. To assess the risk of preinvasive and invasive cervical cancer in a cross-sectional study of women screened for IPV by type, frequency and duration, 1152 women ages 18–65 were recruited from family practice clinics in 1997–1998. They were screened for IPV during a brief in-clinic interview, and health history and current status were assessed in a follow-up interview. Of 1152 …
The Tostan Story: Breakthrough In Senegal Ending Female Genital Cutting, Population Council
The Tostan Story: Breakthrough In Senegal Ending Female Genital Cutting, Population Council
Reproductive Health
In Senegal, elements of the Tostan education program were fundamental to the ending of the practice of female genital cutting (FGC) which has persisted for centuries, despite outside efforts to abolish such a dangerous and harmful operation. This paper is an attempt by those who lived through these events to share the experience with others. At a time when there has been little reduction in the numbers of women ending FGC in 28 African countries, when different programs and strategies have rarely succeeded in making an impact on the number of women still practicing FGC despite laws abolishing the tradition, …
Attitudes, Practices And Knowledge Regarding Cervical Cancer Screening Among Greek Women In The Area Of Perth, Panagiota Karnaki
Attitudes, Practices And Knowledge Regarding Cervical Cancer Screening Among Greek Women In The Area Of Perth, Panagiota Karnaki
Theses: Doctorates and Masters
The purpose of this thesis is to study the attitudes, practices and knowledge of Greek women in Perth, Western Australia, regarding cervical cancer screening. This is important because no study has yet examined the cervical screening pattern of this group, despite their low participation rate in screening programs. Qualitative semi-structured interviews among 15 Greek women in Perth were used for data collection. Interviews were conducted both in English and Greek. Eight out of the 15 women interviewed did not participate in frequent screening and many had had only one Pap test in their life. Culture and religion influenced negative attitudes …
Telomerase Activity In Human Preimplantation Embryos, Diane Lynne Wright
Telomerase Activity In Human Preimplantation Embryos, Diane Lynne Wright
Theses and Dissertations in Biomedical Sciences
Telomerase, a ribonucleoprotein, has been described as an essential component of highly proliferative cells, which stabilizes the telomeres and avoids cellular senescence. Telomerase has been identified in various embryonic cell stages, hematopoietic cells, and in >85% of tumor tissue biopsies analyzed. The ability to measure the potential to proliferate successfully could provide an objective measure of an embryo's quality. The objectives of this study were to modify the telomeric repeat amplification protocol (TRAP) assay system for increased sensitivity to allow detection of telomerase activity in the single cell of an oocyte and embryo, obtain telomerase activity levels for the oocyte …
Sterilization In The United States: Prevalence And Controversies, Carey Brown
Sterilization In The United States: Prevalence And Controversies, Carey Brown
Masters Theses & Specialist Projects
There have been many breakthroughs in birth control technologies, many of which have been beneficial for women. However, many feminists who advocate reproductive freedom also warn that freedom for some might lead to further oppression for others. The case in point is the practice of tubal ligation in the United States. Conflict theory indicates that the field of medicine is a social structure that is based upon capitalistic ideology and serves to perpetuate inequality. Feminist theory argues that medicine systematically disempowers women and that notions of family are very narrowly defined. This study examined the prevalence of tubal ligation among …
Targeted Endovascular Therapy Combined With Angiographic Wound Blush As A Novel Predictor For Limb Salvage In Patients With Critical Limb Ischemia, Abdullah Al-Mallah, Mohamed Abdelhamid, Mohamed Hamza
Targeted Endovascular Therapy Combined With Angiographic Wound Blush As A Novel Predictor For Limb Salvage In Patients With Critical Limb Ischemia, Abdullah Al-Mallah, Mohamed Abdelhamid, Mohamed Hamza
Al-Azhar International Medical Journal
Abstract: Background: Chronic threatening limb ischemia (CTLI) is a major medical problem affecting limbs, quality of life and survival. Angioplasty is considered as the first choice for treatment. Neither definite strategy was defined as the best option nor intraoperative endpoint for successful angioplasty for infragenicular vessles. Aim: to investigate angiosomal concept and wound blush in wound healing after infra-genicular angioplasty. Methods: 40 patients with CTLI affecting the infra-genicular arteries. Patients were divided into two groups; Direct revascularization (DR) and Indirect Revascularization groups (This was done if the direct revascularization was not technically possible). According to post intervention wound blush, patients …
The Immunology Of Parturition, Ari Mayer Mackler
The Immunology Of Parturition, Ari Mayer Mackler
Loma Linda University Electronic Theses, Dissertations & Projects
Macrophage-derived factors have been implicated in the etiology of preterm labor but little is known about trafficking of this immune cell into the uterus or its regulation of uterine contractility in mice. Enhanced number and activation of macrophages was hypothesized to precede parturition. Studies characterized contractile activity and the distribution of macrophages in the uterus of C3/HeN mice before and after term (=day 19). Contractile activity by uterine strips on days 15 and 18 of pregnancy, the day of delivery, and 1 day postpartum indicate that an endogenous oscillator sustains high frequency contractions. Modest acceleration of the pacemaker occurred before …
Physical Violence During Pregnancy: Maternal Complications And Birth Outcomes, Vilma E. Cokkinides, Ann L. Coker, Maureen Sanderson, Cheryl Addy, Lesa Bethea
Physical Violence During Pregnancy: Maternal Complications And Birth Outcomes, Vilma E. Cokkinides, Ann L. Coker, Maureen Sanderson, Cheryl Addy, Lesa Bethea
CRVAW Faculty Journal Articles
Objective: To assess the association between physical violence during the 12 months before delivery and maternal complications and birth outcomes.
Methods: We used population-based data from 6143 women who delivered live-born infants between 1993 and 1995 in South Carolina. Data on women's physical violence during pregnancy were based on self-reports of partner-inflicted physical hurt and being involved in a physical fight. Outcome data included maternal antenatal hospitalizations, labor and delivery complications, low birth weights, and preterm births. Odds ratios and 95% confidence intervals were calculated to measure the associations between physical violence, maternal morbidity, and birth outcomes.
Results: The prevalence …
Ovulatory And Metabolic Effects Of D-Chiro-Inositol In The Polycystic Ovary Syndrome, John E. Nestler, Daniela J. Jakubowicz , M.D., Paula Reamer , M.A., Ronald D. Gunn , M.S., Geoffrey Allan , Ph. D.
Ovulatory And Metabolic Effects Of D-Chiro-Inositol In The Polycystic Ovary Syndrome, John E. Nestler, Daniela J. Jakubowicz , M.D., Paula Reamer , M.A., Ronald D. Gunn , M.S., Geoffrey Allan , Ph. D.
Obstetrics and Gynecology Publications
BACKGROUND
Women with the polycystic ovary syndrome have insulin resistance and hyperinsulinemia, possibly because of a deficiency of a d-chiro-inositol–containing phosphoglycan that mediates the action of insulin. We hypothesized that the administration of d-chiro-inositol would replenish stores of the mediator and improve insulin sensitivity.
METHODS
We measured steroids in serum and performed oral glucose-tolerance tests before and after the oral administration of 1200 mg of d-chiro-inositol or placebo once daily for six to eight weeks in 44 obese women with the polycystic ovary syndrome. The serum progesterone concentration was measured weekly to monitor for …
Igf Paracrine And Autocrine Interactions Between Conceptus And Oviduct., A J Watson, M E Westhusin, Q A Winger
Igf Paracrine And Autocrine Interactions Between Conceptus And Oviduct., A J Watson, M E Westhusin, Q A Winger
Obstetrics & Gynaecology Publications
Development in vitro is influenced by embryo density, serum, somatic cell co-culture and the production of 'embryotrophic' paracrine and autocrine factors. Research in our laboratory has focussed principally on the insulin-like growth factor (IGF) family. We have demonstrated that pre-attachment bovine and ovine embryos express mRNAs encoding a number of growth factor ligand and receptor genes including all members of the IGF ligand and receptor family throughout this developmental interval. In addition, early embryos express mRNAs encoding IGF-binding proteins (IGFBPs) 2-5 from the one-cell to the blastocyst stage and IGFBP5 mRNA at the blastocyst stage. Cultured bovine blastocysts release up …
The Tostan Story: Breakthrough In Senegal Ending Female Genital Cutting [Arabic], Population Council
The Tostan Story: Breakthrough In Senegal Ending Female Genital Cutting [Arabic], Population Council
Reproductive Health
In Senegal, elements of the Tostan education program were fundamental to the ending of the practice of female genital cutting (FGC) which has persisted for centuries, despite outside efforts to abolish such a dangerous and harmful operation. This paper is an attempt by those who lived through these events to share the experience with others. At a time when there has been little reduction in the numbers of women ending FGC in 28 African countries, when different programs and strategies have rarely succeeded in making an impact on the number of women still practicing FGC despite laws abolishing the tradition, …
Effect Of Prepregnancy Body Mass Index And Gestational Weight Gain On Birth Weight, S S. Merchant, I A. Momin, A A. Sewani, N F. Zuberi
Effect Of Prepregnancy Body Mass Index And Gestational Weight Gain On Birth Weight, S S. Merchant, I A. Momin, A A. Sewani, N F. Zuberi
Department of Obstetrics & Gynaecology
Objective: Nutritional status of women has been considered an important prognostic indicator of birth outcome. The study aims to show the effect of various prepregnancy Body Mass Index (BMI) categories and corresponding gestational weight gain on newborn birth weight.
Methods: Two hundred women were included in the study. These women had regular antenatal visits and later delivered at The Aga Khan University Hospital (AKUH) between the period January 1, 1996 to December 31, 1997.
Results: For women with prepregnancy BMI < 19., mean birth weight of newborns was lower for those gaining < 12.5 kg than those gaining > 12.5 kg (P < 0.001). Women who started their pregnancy with BMI 19.8-26 and gained weight above expected range gave birth to high birth weight babies (P = 0.009). Gestational weight gain did not have a significant association with birth weight for women having prepregnancy BMI > 26.
Conclusion: Efforts should be made to attain adequate prepregnancy weight to reduce the likelihood of low …
Trophectoderm Differentiation In The Bovine Embryo: Characterization Of A Polarized Epithelium., L C Barcroft, A Hay-Schmidt, A Caveney, E Gilfoyle, E W Overstrom, P Hyttel, A J Watson
Trophectoderm Differentiation In The Bovine Embryo: Characterization Of A Polarized Epithelium., L C Barcroft, A Hay-Schmidt, A Caveney, E Gilfoyle, E W Overstrom, P Hyttel, A J Watson
Obstetrics & Gynaecology Publications
Blastocytst formation is dependent on the differentiation of a transporting epithelium, the trophectoderm, which is coordinated by the embryonic expression and cell adhesive properties of E-cadherin. The trophectoderm shares differentiative characteristics with all epithelial tissues, including E-cadherin-mediated cell adhesion, tight junction formation, and polarized distribution of intramembrane proteins, including the Na-K ATPase. The present study was conducted to characterize the mRNA expression and distribution of polypeptides encoding E-cadherin, beta-catenin, and the tight junction associated protein, zonula occludens protein 1, in pre-attachment bovine embryos, in vitro. Immunocytochemistry and gene specific reverse transcription--polymerase chain reaction methods were used. Transcripts for E-cadherin and …
Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz
Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz
Obstetrics & Gynaecology Publications
In the present study the abundance of mRNAs for eukaryotic translation initiation factors eIF-1A (formerly known as eIF-4C), -2alpha, -4A, -4E, and -5 was examined in in vivo-derived mouse embryos throughout preimplantation development using a semiquantitative reverse transcription-polymerase chain reaction assay. Although the mRNA profile for each gene is unique, only mRNA for eIF-1A transiently increases during embryonic gene activation (EGA) at the 2-cell stage, and this was confirmed by an independent hybridization-based assay. In in vitro-developed bovine embryos, mRNA for eIF-1A was transiently detected at the 8-cell stage, when the major activation of the genome occurs in this species. …
Leukemia Inhibitory Factor (Lif): Murine Preimplantation Embryo Development, Implantation Rates, And Skeletal Development, Michael Hayes Mitchell
Leukemia Inhibitory Factor (Lif): Murine Preimplantation Embryo Development, Implantation Rates, And Skeletal Development, Michael Hayes Mitchell
Theses and Dissertations in Biomedical Sciences
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine which demonstrates perplexing physiological effects. It has been demonstrated that LIF is essential for implantation in mice. Little is known relating to the manner by which LIF effects pre-implantation and post-implantation development. The objectives of this project were to determine the effects LIF on pre-implantation development, to determine the effects that it may have on implantation rates, successful pregnancy rates, and resorption rates, and to determine the effects that LIF has on the skeletal development of mice. For the embryo transfer experiments, embryos were exposed to test compounds in the transfer medium …
Mouse Embryo Development In The Presence Of Capsaicin, Carlos Santiago Villar-Gosalvez
Mouse Embryo Development In The Presence Of Capsaicin, Carlos Santiago Villar-Gosalvez
Theses and Dissertations in Biomedical Sciences
Capsaicin is the pungent agent found in hot peppers of the Capsicum genus. It is a potent neurotoxin that stimulates the degranulation and degeneration of C-afferent neurons. Capsaicin is widely used as a food condiment and medicine. Human exposure of capsaicin can exceed levels shown to be neurotoxic in laboratory animals. Additionally, capsaicin can cross the blood/placenta barrier and affect an embryo in utero. In order to assay the potential for toxicity to human embryos, mouse embryos were exposed to capsaicin and the effect of the capsaicin on embryo development was measured. Embryos were co-cultured in Krebs medium with 1% …
Expression, Isolation And Purification Of Human Zona Pellucida Protein 3, Ting-Fung Chi
Expression, Isolation And Purification Of Human Zona Pellucida Protein 3, Ting-Fung Chi
Theses and Dissertations in Biomedical Sciences
Mammalian fertilization involves interactions of sperm surface receptors with the ligands of the zona pellucida, an extracellular matrix surrounding the ovulated oocytes. In humans, the zona pellucida is composed of three major glycoproteins. One of them, ZP3, participates in the primary sperm binding and in the subsequent triggering of the spermatozoa's acrosome reaction. Studies on the role of this specific protein in the human fertilization process are hampered by the limited amount of available biologically functional proteins.
By use of a pcDNA 3.1(+) expression vector, a transfecting-vector was constructed containing a 1.3 kb histidine tagged hZP3 cDNA. This histidine tagged …
Na/K-Atpase-Mediated 86rb+ Uptake And Asymmetrical Trophectoderm Localization Of Alpha1 And Alpha3 Na/K-Atpase Isoforms During Bovine Preattachment Development., D H Betts, L C Barcroft, A J Watson
Na/K-Atpase-Mediated 86rb+ Uptake And Asymmetrical Trophectoderm Localization Of Alpha1 And Alpha3 Na/K-Atpase Isoforms During Bovine Preattachment Development., D H Betts, L C Barcroft, A J Watson
Obstetrics & Gynaecology Publications
This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-subunit polypeptides were detected by immunofluorescence to encircle the entire cell margins of each blastomere of inseminated zygotes, cleavage stage embryos, and morulae. Immunofluorescent localization of alpha1-subunit polypeptide in bovine blastocysts revealed an alpha1 immunofluorescence signal confined to the basolateral membrane margins of the trophectoderm and encircling the cell periphery of each inner cell mass (ICM) cell. In contrast, alpha …
The Role Of Glycoconjugates In Mediating Human Fertilization And Induction Of Fetomaternal Tolerance, Manish S. Patankar
The Role Of Glycoconjugates In Mediating Human Fertilization And Induction Of Fetomaternal Tolerance, Manish S. Patankar
Theses and Dissertations in Biomedical Sciences
Using the hemizona assay (HZA), a in vitro sperm-egg binding assay, we show that specific glycoconjugates known to inhibit immune cell interactions mediated by the selectins, potently block human sperm-egg binding. The selectin ligand sialyl Lewisx inhibits sperm binding in the HZA by 60% at a concentration of 1 mg/ml. Our data indicates that glycodelin-A, a endometrial glycoprotein known to block sperm-egg binding in the HZA at low concentrations expresses unusual fucosylated lacdiNAc type glycans. The fucosylated lacdiNAc type sugars have been previously shown to be 15-20 fold more potent ligands of E-selectin. Glycodelin-S a seminal plasma glycoform of …